GT622580

EST Overview


Name	GT622580
Unique Name	GT622580
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	726

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT622580 vs. TrEMBL
Match: B7FMN3_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 362.459 bits (929), Expect = 2.044e-98
Identity = 180/186 (96.77%), Postives = 184/186 (98.92%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PVAPR+GD+IFANIERVNAELFTLTYGAIVRQLLTDLEEV+EVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASV+NWDAEGT CSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE
Sbjct:    1 MAPVAPRTGDAIFANIERVNAELFTLTYGAIVRQLLTDLEEVDEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVTNWDAEGTTCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: C6SXA4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 353.214 bits (905), Expect = 1.240e-95
Identity = 173/186 (93.01%), Postives = 179/186 (96.24%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            MPPVAPRSGD+IFAN+ERVNAELFTLTYGAIVRQLLTDLEEVEEVN+QLDQMGYNIGIRLIDEFLAKSNVSRC DFRET DVIAKVGFKMFLGV ASV+NWDA+GTCCSI+LEDNPLVDFVELPDN QGLYYCNILSGVIRGALDMVSMK EVTWLRD LRGDDVFELQVKLLK VPEEYPYKDDE
Sbjct:    1 MPPVAPRSGDAIFANVERVNAELFTLTYGAIVRQLLTDLEEVEEVNEQLDQMGYNIGIRLIDEFLAKSNVSRCNDFRETTDVIAKVGFKMFLGVIASVTNWDADGTCCSIVLEDNPLVDFVELPDNCQGLYYCNILSGVIRGALDMVSMKTEVTWLRDVLRGDDVFELQVKLLKHVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: C6SYG5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 352.829 bits (904), Expect = 1.620e-95
Identity = 173/186 (93.01%), Postives = 178/186 (95.70%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            MPPVAPRSGD+IFAN+ERVNAELFTLTYGAIVRQLLTDLEE E VNKQLDQMGYNIGIRLIDEFLAKSNVSRC DFRET DVIAKVGFKMFLGVTASV+NWDA+GTCCSI+LEDNPLVDFVELPDN QGLYYCNILSGVIRGALDMVSMK EVTWLRD LRGDDVFELQVKLLK VPEEYPYKDDE
Sbjct:    1 MPPVAPRSGDAIFANVERVNAELFTLTYGAIVRQLLTDLEEAEVVNKQLDQMGYNIGIRLIDEFLAKSNVSRCNDFRETTDVIAKVGFKMFLGVTASVTNWDADGTCCSIVLEDNPLVDFVELPDNCQGLYYCNILSGVIRGALDMVSMKTEVTWLRDVLRGDDVFELQVKLLKHVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: A9PCZ9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834624 PE=2 SV=1)

HSP 1 Score: 344.739 bits (883), Expect = 4.411e-93
Identity = 167/186 (89.78%), Postives = 178/186 (95.70%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PVAPRSGD+IFA++E VNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIG+RLIDEFLAKSNVSRCVDF+ETADVIAKVGFKMFLGV ASV+NWDA+GTCCSI+LEDNPLVDFVELPD  QGLYYCNILSGV+RGAL+MVSMK EVTW+RD LRGDD FELQVKLLKQVPEEYPYKDDE
Sbjct:    1 MAPVAPRSGDAIFASVEHVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGVRLIDEFLAKSNVSRCVDFKETADVIAKVGFKMFLGVGASVTNWDADGTCCSIVLEDNPLVDFVELPDTCQGLYYCNILSGVVRGALEMVSMKTEVTWVRDMLRGDDAFELQVKLLKQVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: B9SA27_RICCO (Trafficking protein particle complex subunit, putative OS=Ricinus communis GN=RCOM_0183430 PE=4 SV=1)

HSP 1 Score: 343.584 bits (880), Expect = 9.827e-93
Identity = 167/186 (89.78%), Postives = 178/186 (95.70%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PV PRSGD+IFA++ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRL+DEFLAKSNV+RCVDFRETA+VIAKVGFKMFLGV+ASV+NWDA+GTCCSIILEDNPLVDFVELPD  QGLYYCNILSGVIRGAL+MVSMK EVTW RD LRGDD +ELQVKLLKQVPEEYPYKDDE
Sbjct:    1 MAPVGPRSGDAIFASVERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLVDEFLAKSNVNRCVDFRETAEVIAKVGFKMFLGVSASVTNWDADGTCCSIILEDNPLVDFVELPDTCQGLYYCNILSGVIRGALEMVSMKTEVTWARDMLRGDDAYELQVKLLKQVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: A5AU19_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041653 PE=4 SV=1)

HSP 1 Score: 341.658 bits (875), Expect = 3.734e-92
Identity = 165/186 (88.71%), Postives = 177/186 (95.16%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M P APRSGD+IFAN+ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDF+ETA+ IAKVGFKMFLGVTASV+NWDA+GT CS++LEDNPLVDFVELPD  QGLYYCNILSGV+RGAL+MVSMK EVTW+RD LRGDD +ELQVKLLKQVPEEYPYKDDE
Sbjct:    1 MAPAAPRSGDAIFANVERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFKETAEAIAKVGFKMFLGVTASVTNWDADGTSCSLVLEDNPLVDFVELPDTCQGLYYCNILSGVVRGALEMVSMKTEVTWIRDMLRGDDAYELQVKLLKQVPEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: D7MUC9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_357730 PE=4 SV=1)

HSP 1 Score: 335.495 bits (859), Expect = 2.676e-90
Identity = 164/186 (88.17%), Postives = 176/186 (94.62%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PV PRSGD+IF++I+RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKS VSRCVDF+ETA++IAKVGFKMFLGVTASV++WDA+GTCCSIILEDNPLVDFVELPD  QGLYYCNILSGVIRGAL+MVSMK EVTW RD LRGDD +ELQVKLLKQV EEYPYKDDE
Sbjct:    1 MAPVGPRSGDAIFSSIDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSGVSRCVDFKETAEMIAKVGFKMFLGVTASVTSWDADGTCCSIILEDNPLVDFVELPDTCQGLYYCNILSGVIRGALEMVSMKTEVTWTRDVLRGDDAYELQVKLLKQVAEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: Q9FFV2_ARATH (AT5g54750/MBG8_1 OS=Arabidopsis thaliana GN=At5g54750 PE=2 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 7.786e-90
Identity = 162/186 (87.10%), Postives = 176/186 (94.62%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PV PRSGD+IF++I+RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKS VSRCVDF+ETA++IAKVGFKMFLGVTASV++WD++GTCCSIILEDNPLVDFVELPD  QGLYYCN+LSGVIRGAL+MVSMK EVTW RD LRGDD +ELQVKLLKQV EEYPYKDDE
Sbjct:    1 MAPVGPRSGDAIFSSIDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSGVSRCVDFKETAEMIAKVGFKMFLGVTASVTSWDSDGTCCSIILEDNPLVDFVELPDTCQGLYYCNVLSGVIRGALEMVSMKTEVTWTRDVLRGDDAYELQVKLLKQVAEEYPYKDDE 186

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BLAST of GT622580 vs. TrEMBL
Match: B4FFB0_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 328.176 bits (840), Expect = 4.272e-88
Identity = 157/186 (84.41%), Postives = 178/186 (95.70%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            MPP AP+SGD++FA+++RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIG RL+DEFLAKSNVSRCVDF+ETADVIAK+GFKMFLGVTA+V+NWDAEGT CS++LEDNPLVDFVELPD  QGL YCN+LSGVIRGAL+MVSMKAEVTW+RD LRGDD +E++VKL+KQVPEEYPYKDD+
Sbjct:    1 MPP-APKSGDALFASVDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGTRLVDEFLAKSNVSRCVDFKETADVIAKLGFKMFLGVTATVTNWDAEGTSCSLVLEDNPLVDFVELPDTCQGLQYCNLLSGVIRGALEMVSMKAEVTWVRDMLRGDDAYEMRVKLIKQVPEEYPYKDDD 185

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BLAST of GT622580 vs. TrEMBL
Match: B6T6G9_MAIZE (Trafficking protein particle complex subunit 3 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 7.287e-88
Identity = 157/185 (84.86%), Postives = 177/185 (95.68%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDD 590
            MPP AP+SGD++FA+++RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIG RL+DEFLAKSNVSRCVDF+ETADVIAK+GFKMFLGVTA+V+NWDAEGT CS++LEDNPLVDFVELPD  QGL YCN+LSGVIRGAL+MVSMKAEVTW+RD LRGDD +E++VKL+KQVPEEYPYKDD
Sbjct:    1 MPP-APKSGDALFASVDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGTRLVDEFLAKSNVSRCVDFKETADVIAKLGFKMFLGVTATVTNWDAEGTSCSLVLEDNPLVDFVELPDTCQGLQYCNLLSGVIRGALEMVSMKAEVTWVRDMLRGDDAYEMRVKLIKQVPEEYPYKDD 184

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_CHICK (Trafficking protein particle complex subunit 3 OS=Gallus gallus GN=TRAPPC3 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.267e-54
Identity = 98/165 (59.39%), Postives = 133/165 (80.61%), Query Frame = 3

Query:   84 ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYP 578
            +++N+ELFTLTYGA+V QL  D E  E+VNKQLD+MGYNIG+RLI++FLA+SNV RC DFRETADVIAK+ FKM+LG+T S++NW   G   S+ILE+NPLVDFVELPDN+  L Y N+L GV+RGAL+MV M  +V +++D L+GD V E+++K ++++ +  P
Sbjct:   12 KKMNSELFTLTYGALVTQLCKDYENDEDVNKQLDKMGYNIGVRLIEDFLARSNVGRCHDFRETADVIAKIAFKMYLGITPSITNWSPGGDEFSLILENNPLVDFVELPDNHSSLIYSNLLCGVLRGALEMVQMAVDVKFVQDTLKGDSVTEIRMKFIRRIEDNLP 176

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_RAT (Trafficking protein particle complex subunit 3 OS=Rattus norvegicus GN=Trappc3 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.919e-53
Identity = 97/165 (58.79%), Postives = 132/165 (80.00%), Query Frame = 3

Query:   84 ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYP 578
            +++++ELFTLTYGA+V QL  D E  E+VNKQLD+MGYNIG+RLI++FLA+SNV RC DFRETADVIAKV FKM+LG+T S++NW   G   S+ILE+NPLVDFVELPDN+  L Y N+L GV+RGAL+MV M  E  +++D L+GD V E++++ ++++ +  P
Sbjct:   12 KKMSSELFTLTYGALVTQLCKDYENDEDVNKQLDRMGYNIGVRLIEDFLARSNVGRCHDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELPDNHSALIYSNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNLP 176

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_MOUSE (Trafficking protein particle complex subunit 3 OS=Mus musculus GN=Trappc3 PE=1 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.919e-53
Identity = 97/165 (58.79%), Postives = 132/165 (80.00%), Query Frame = 3

Query:   84 ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYP 578
            +++++ELFTLTYGA+V QL  D E  E+VNKQLD+MGYNIG+RLI++FLA+SNV RC DFRETADVIAKV FKM+LG+T S++NW   G   S+ILE+NPLVDFVELPDN+  L Y N+L GV+RGAL+MV M  E  +++D L+GD V E++++ ++++ +  P
Sbjct:   12 KKMSSELFTLTYGALVTQLCKDYENDEDVNKQLDRMGYNIGVRLIEDFLARSNVGRCHDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELPDNHSALIYSNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNLP 176

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_HUMAN (Trafficking protein particle complex subunit 3 OS=Homo sapiens GN=TRAPPC3 PE=1 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 5.583e-53
Identity = 96/165 (58.18%), Postives = 132/165 (80.00%), Query Frame = 3

Query:   84 ERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYP 578
            +++++ELFTLTYGA+V QL  D E  E+VNKQLD+MG+NIG+RLI++FLA+SNV RC DFRETADVIAKV FKM+LG+T S++NW   G   S+ILE+NPLVDFVELPDN+  L Y N+L GV+RGAL+MV M  E  +++D L+GD V E++++ ++++ +  P
Sbjct:   12 KKMSSELFTLTYGALVTQLCKDYENDEDVNKQLDKMGFNIGVRLIEDFLARSNVGRCHDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELPDNHSSLIYSNLLCGVLRGALEMVQMAVEAKFVQDTLKGDGVTEIRMRFIRRIEDNLP 176

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_DICDI (Trafficking protein particle complex subunit 3 OS=Dictyostelium discoideum GN=trappc3 PE=3 SV=2)

HSP 1 Score: 202.601 bits (514), Expect = 1.796e-51
Identity = 100/174 (57.47%), Postives = 132/174 (75.86%), Query Frame = 3

Query:   60 GDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQ-GLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYP 578
            G+ +F  +E++N+ELFTLTYGA+V QL+ D E++E+VN +L+QMGYNIGIRLI+EFLAKS + RC DF ETA+VIAKVGFKMFLGV A V +WDA      + +EDNPL+DFVELPD Y+  LYY NIL GV+RGAL+MV MK + T+++  L  D   E++V L + + +  P
Sbjct:    9 GNDVFNKVEKINSELFTLTYGALVTQLIKDYEDIEQVNIKLEQMGYNIGIRLIEEFLAKSGIGRCSDFIETAEVIAKVGFKMFLGVNAHVGDWDANRKEFHLTIEDNPLIDFVELPDQYKHKLYYSNILCGVMRGALEMVQMKVKCTFVKCTLSDDSTSEIKVVLEEVLSDMIP 182

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BLAST of GT622580 vs. SwissProt
Match: BET3_SCHPO (Transport protein particle subunit bet3 OS=Schizosaccharomyces pombe GN=bet3 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 4.285e-45
Identity = 92/178 (51.69%), Postives = 128/178 (71.91%), Query Frame = 3

Query:   60 GDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELP-DNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDD 590
            G+ ++  +++VNAELF LTYG+IV QL  D+   E+VN++LD+MGYNIGIRLI++FLAK+   RC DFRET + +AKVGFK+FL  +  +S+   +G    + L+DNPL +FVELP D  Q L+Y NIL GVIRGAL+M+ M  +  +LRD LRGD+  E++V L + + EE P  D+
Sbjct:    7 GEDVYKKVDKVNAELFVLTYGSIVAQLCKDMN-YEKVNEELDKMGYNIGIRLIEDFLAKTEWPRCADFRETGETVAKVGFKVFLNFSPIISSVSDDGNTFVLTLDDNPLAEFVELPADARQKLWYSNILCGVIRGALEMLQMDVDAVFLRDILRGDEHTEIRVHLKRILKEEIPPGDE 183

[Close]

BLAST of GT622580 vs. SwissProt
Match: TPC3L_HUMAN (Trafficking protein particle complex subunit 3-like protein OS=Homo sapiens GN=BET3L PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.433e-40
Identity = 84/163 (51.53%), Postives = 118/163 (72.39%), Query Frame = 3

Query:   87 RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFV-ELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEE 572
            ++N +LF LTYGA+V QL  D E+ E+VN+ LD+MGY IG RL+++FLA+S V RC  + E  D+IA+V FKM+LG+T SV+  ++     S+ILE NPLV+FV ELP     L YCN+L G+IRGAL+MV + A+VT+L+D L+GD V E+ +  LK+  E+
Sbjct:   13 KINKDLFVLTYGALVAQLCKDYEKDEDVNQYLDKMGYGIGTRLVEDFLARSCVGRCHSYSEIIDIIAQVAFKMYLGITPSVTCNNSSKNEFSLILEKNPLVEFVEELPAGRSSLCYCNLLCGIIRGALEMVHLAADVTFLQDRLKGDSVTEIGITFLKKRDEK 175

[Close]

BLAST of GT622580 vs. SwissProt
Match: TPC3L_MOUSE (Trafficking protein particle complex subunit 3-like protein OS=Mus musculus GN=Bet3l PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.178e-40
Identity = 83/163 (50.92%), Postives = 117/163 (71.78%), Query Frame = 3

Query:   87 RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFV-ELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEE 572
            ++N +LF LTYGA+V QL  D E+ E+VNK LD+MGYNIG RL+++FLA+S V RC  + E  ++IA+V FKM+LG+T SV+  ++     S+IL  NPL +FV ELP     L YCN+L G+IRGAL+MV + A VT+L+D L+GD V E+ +  LK++ E+
Sbjct:   13 KINKDLFVLTYGALVAQLCKDYEKDEDVNKYLDKMGYNIGTRLVEDFLARSCVRRCHSYSEIINIIAQVAFKMYLGITPSVTCHNSSRNEFSLILHKNPLAEFVEELPAGRSALCYCNLLCGIIRGALEMVHLAANVTFLQDRLKGDSVTEIGITFLKKLDEK 175

[Close]

BLAST of GT622580 vs. SwissProt
Match: BET3_YEAST (Transport protein particle 22 kDa subunit OS=Saccharomyces cerevisiae GN=BET3 PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.690e-39
Identity = 83/180 (46.11%), Postives = 125/180 (69.44%), Query Frame = 3

Query:   60 GDSIFAN-IERVNAELFTLTYGAIVRQLLTDLE-EVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELP-DNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDD 590
            G+ I+ N  E++N ELFTLTYG+IV QL  D E +  +VN  L  MGYNIG RLI++FLA++ + RC +  +T++V++K  FK+FL +T +++NW       S+IL++NPL DFVELP D  + L+Y NIL GV++G+L+MV +  +V ++ D LRGD   E++VKL + + +E P  +D
Sbjct:   14 GEEIWKNKTEKINTELFTLTYGSIVAQLCQDYERDFNKVNDHLYSMGYNIGCRLIEDFLARTALPRCENLVKTSEVLSKCAFKIFLNITPNITNWSHNKDTFSLILDENPLADFVELPMDAMKSLWYSNILCGVLKGSLEMVQLDCDVWFVSDILRGDSQTEIKVKLNRILKDEIPIGED 193

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BLAST of GT622580 vs. SwissProt
Match: TPPC3_CAEBR (Probable trafficking protein particle complex subunit 3 OS=Caenorhabditis briggsae GN=CBG10062 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.644e-28
Identity = 65/140 (46.43%), Postives = 96/140 (68.57%), Query Frame = 3

Query:   75 ANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAK-SNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNY--QGLYYCNILSGVIRGALDMVSMK 485
            A+ ++++AELF LTYGA+V ++L D E+ ++V  QLD+MG+N+G RL D+FLAK +NV RCVD R+ ADV+ +     +LGV+A+ S+W +      I LE NPL + V++P N    GL Y  +++G IRGAL+ V  K
Sbjct:   10 ADSKKMSAELFCLTYGAMVTEMLKDYEDPKDVTVQLDKMGFNMGTRLADDFLAKNANVPRCVDTRQIADVLCRNAIPCYLGVSATASSWSSGDREFIITLESNPLTELVQVPPNLVSAGLSYSQMIAGAIRGALEAVHFK 149

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BLAST of GT622580 vs. TAIR peptide
Match: AT5G54750.1 (| Symbols: | Transport protein particle (TRAPP) component | chr5:22242080-22243477 FORWARD LENGTH=186)

HSP 1 Score: 333.954 bits (855), Expect = 5.216e-92
Identity = 162/186 (87.10%), Postives = 176/186 (94.62%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PV PRSGD+IF++I+RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKS VSRCVDF+ETA++IAKVGFKMFLGVTASV++WD++GTCCSIILEDNPLVDFVELPD  QGLYYCN+LSGVIRGAL+MVSMK EVTW RD LRGDD +ELQVKLLKQV EEYPYKDDE
Sbjct:    1 MAPVGPRSGDAIFSSIDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMGYNIGIRLIDEFLAKSGVSRCVDFKETAEMIAKVGFKMFLGVTASVTSWDSDGTCCSIILEDNPLVDFVELPDTCQGLYYCNVLSGVIRGALEMVSMKTEVTWTRDVLRGDDAYELQVKLLKQVAEEYPYKDDE 186

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BLAST of GT622580 vs. TAIR peptide
Match: AT5G54750.2 (| Symbols: | Transport protein particle (TRAPP) component | chr5:22242080-22243477 FORWARD LENGTH=199)

HSP 1 Score: 324.709 bits (831), Expect = 3.165e-89
Identity = 162/199 (81.41%), Postives = 176/199 (88.44%), Query Frame = 3

Query:   36 MPPVAPRSGDSIFANIERVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQM-------------GYNIGIRLIDEFLAKSNVSRCVDFRETADVIAKVGFKMFLGVTASVSNWDAEGTCCSIILEDNPLVDFVELPDNYQGLYYCNILSGVIRGALDMVSMKAEVTWLRDALRGDDVFELQVKLLKQVPEEYPYKDDE 593
            M PV PRSGD+IF++I+RVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQM             GYNIGIRLIDEFLAKS VSRCVDF+ETA++IAKVGFKMFLGVTASV++WD++GTCCSIILEDNPLVDFVELPD  QGLYYCN+LSGVIRGAL+MVSMK EVTW RD LRGDD +ELQVKLLKQV EEYPYKDDE
Sbjct:    1 MAPVGPRSGDAIFSSIDRVNAELFTLTYGAIVRQLLTDLEEVEEVNKQLDQMYSSSTIYVDSLPWGYNIGIRLIDEFLAKSGVSRCVDFKETAEMIAKVGFKMFLGVTASVTSWDSDGTCCSIILEDNPLVDFVELPDTCQGLYYCNVLSGVIRGALEMVSMKTEVTWTRDVLRGDDAYELQVKLLKQVAEEYPYKDDE 199

The following BLAST results are available for this feature:

BLAST of GT622580 vs. TrEMBL
BLAST of GT622580 vs. SwissProt
BLAST of GT622580 vs. TAIR peptide

BLAST of GT622580 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FMN3_MEDTR	2.044e-98	96.77	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6SXA4_SOYBN	1.240e-95	93.01	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SYG5_SOYBN	1.620e-95	93.01	Putative uncharacterized protein OS=Glycine max PE...	[more]
A9PCZ9_POPTR	4.411e-93	89.78	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SA27_RICCO	9.827e-93	89.78	Trafficking protein particle complex subunit, puta...	[more]
A5AU19_VITVI	3.734e-92	88.71	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7MUC9_ARALY	2.676e-90	88.17	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9FFV2_ARATH	7.786e-90	87.10	AT5g54750/MBG8_1 OS=Arabidopsis thaliana GN=At5g54...	[more]
B4FFB0_MAIZE	4.272e-88	84.41	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6T6G9_MAIZE	7.287e-88	84.86	Trafficking protein particle complex subunit 3 OS=...	[more]

BLAST of GT622580 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TPPC3_CHICK	2.267e-54	59.39	Trafficking protein particle complex subunit 3 OS=...	[more]
TPPC3_RAT	1.919e-53	58.79	Trafficking protein particle complex subunit 3 OS=...	[more]
TPPC3_MOUSE	1.919e-53	58.79	Trafficking protein particle complex subunit 3 OS=...	[more]
TPPC3_HUMAN	5.583e-53	58.18	Trafficking protein particle complex subunit 3 OS=...	[more]
TPPC3_DICDI	1.796e-51	57.47	Trafficking protein particle complex subunit 3 OS=...	[more]
BET3_SCHPO	4.285e-45	51.69	Transport protein particle subunit bet3 OS=Schizos...	[more]
TPC3L_HUMAN	2.433e-40	51.53	Trafficking protein particle complex subunit 3-lik...	[more]
TPC3L_MOUSE	3.178e-40	50.92	Trafficking protein particle complex subunit 3-lik...	[more]
BET3_YEAST	2.690e-39	46.11	Transport protein particle 22 kDa subunit OS=Sacch...	[more]
TPPC3_CAEBR	3.644e-28	46.43	Probable trafficking protein particle complex subu...	[more]

BLAST of GT622580 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G54750.1	5.216e-92	87.10	\| Symbols: \| Transport protein particle (TRAPP) c...	[more]
AT5G54750.2	3.165e-89	81.41	\| Symbols: \| Transport protein particle (TRAPP) c...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 34..45 score
None	No IPR available	GENE3D	3.30.1380.20	no description	coord: 22..178 score: 2.8
None	No IPR available	PANTHER	PTHR13048	TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3	coord: 7..188 score: 2.9e
None	No IPR available	SEG	seg	seg	coord: 11..22 score
None	No IPR available	SEG	seg	seg	coord: 1..18 score
None	No IPR available	SEG	seg	seg	coord: 1..19 score
IPR007194	Transport protein particle (TRAPP) component	PFAM	PF04051	TRAPP	coord: 26..174 score: 1.6
IPR011644	Haem NO binding	SUPERFAMILY	111126	Ligand-binding domain in the NO signalling and Golgi transport	coord: 20..180 score: 3.3
IPR016721	TRAPP I complex, Bet3	PIR	PIRSF018293	TRAPP I complex, Bet3 subunit	coord: 3..188 score: 4.7e

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT622580 ID=GT622580; Name=GT622580; organism=Lens culinaris; type=EST; length=726bp
CACCAAAGCTAAACAAATCTGAGTGAGCTCTAACGATGCCTCCTGTTGCT
CCTCGATCTGGCGATTCGATATTTGCTAACATCGAACGCGTTAATGCGGA
GCTATTTACTCTGACATATGGGGCAATTGTGAGGCAATTGCTTACAGATC
TGGAAGAGGTTGAGGAAGTCAACAAGCAGCTAGATCAAATGGGTTATAAT
ATTGGAATCCGTTTGATTGACGAGTTTTTGGCTAAATCTAATGTCTCAAG
GTGTGTTGATTTCAGAGAGACAGCTGATGTGATTGCAAAGGTTGGTTTTA
AAATGTTCCTCGGTGTTACCGCATCCGTGTCCAATTGGGATGCTGAAGGA
ACATGTTGTAGTATTATTTTGGAAGATAATCCTTTGGTGGACTTTGTTGA
GCTTCCTGACAACTACCAAGGTCTGTACTACTGCAACATATTAAGCGGAG
TTATTAGAGGAGCCTTGGATATGGTATCAATGAAGGCTGAGGTCACTTGG
CTACGTGATGCGCTTAGAGGTGATGATGTGTTTGAGTTGCAAGTAAAACT
TCTCAAGCAAGTTCCAGAAGAGTATCCATATAAGGATGATGAGTAATATT
CTGTTTTGTAATGTTGTACTTCATCATATCAAGTATTAGATTATTATCTT
TCTGCATTGTGTGCATTTTGACAAAACTAAAAATACAAGAGAAAATCTTT
AATTAAATTATAATATTGGGATTTTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004383	guanylate cyclase activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006182	cGMP biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR007194	TRAPP_component
IPR011644	Heme_NO-bd
IPR016721	Bet3

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