AM162135

EST Overview
NameAM162135
Unique NameAM162135
TypeEST
OrganismPisum sativum (pea)
Sequence length200
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of AM162135 vs. TrEMBL
Match: B9N5K3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_582533 PE=4 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 1.812e-26
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNA+EIY SPSGAVHFTAS
Sbjct:  193 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAVEIYDSPSGAVHFTAS 252
[Close]
BLAST of AM162135 vs. TrEMBL
Match: B9GHK2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_550668 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 7.597e-26
Identity = 57/60 (95.00%), Postives = 58/60 (96.67%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVK NLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNA+EIY SPSGAVHFTAS
Sbjct:  193 TLAVKGNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAVEIYDSPSGAVHFTAS 252

HSP 2 Score: 21.557 bits (44), Expect = 7.597e-26
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  249 FTASNNDCGVR 259
[Close]
BLAST of AM162135 vs. TrEMBL
Match: D7L1D2_ARALY (WD-40 repeat family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_897559 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 6.259e-25
Identity = 55/60 (91.67%), Postives = 57/60 (95.00%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD+ LVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  195 TLAVKDDFLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYNKPSGALHFTAS 254

HSP 2 Score: 21.557 bits (44), Expect = 6.259e-25
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  251 FTASNNDCGVR 261
[Close]
BLAST of AM162135 vs. TrEMBL
Match: Q9LG00_ARATH (F20N2.10 OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.796e-24
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAV+D  LVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  193 TLAVRDKFLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYNKPSGALHFTAS 252

HSP 2 Score: 21.557 bits (44), Expect = 1.796e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  249 FTASNNDCGVR 259
[Close]
BLAST of AM162135 vs. TrEMBL
Match: Q9C5N4_ARATH (Putative uncharacterized protein At1g55680 OS=Arabidopsis thaliana GN=At1g55680 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.796e-24
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAV+D  LVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  193 TLAVRDKFLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYNKPSGALHFTAS 252

HSP 2 Score: 21.557 bits (44), Expect = 1.796e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  249 FTASNNDCGVR 259
[Close]
BLAST of AM162135 vs. TrEMBL
Match: Q94AB4_ARATH (AT3g13340/MDC11_13 OS=Arabidopsis thaliana GN=At3g13340 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.042e-24
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD+ LVAGGFQGELICKHLDRPGVSFCSR TYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  195 TLAVKDDFLVAGGFQGELICKHLDRPGVSFCSRMTYDDNAITNAIEIYNKPSGALHFTAS 254

HSP 2 Score: 21.557 bits (44), Expect = 3.042e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  251 FTASNNDCGVR 261
[Close]
BLAST of AM162135 vs. TrEMBL
Match: Q9LTT4_ARATH (WD domain protein-like OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.045e-24
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD+ LVAGGFQGELICKHLDRPGVSFCSR TYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  166 TLAVKDDFLVAGGFQGELICKHLDRPGVSFCSRMTYDDNAITNAIEIYNKPSGALHFTAS 225

HSP 2 Score: 21.557 bits (44), Expect = 3.045e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  222 FTASNNDCGVR 232
[Close]
BLAST of AM162135 vs. TrEMBL
Match: D7KN06_ARALY (WD-40 repeat family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_892567 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.959e-24
Identity = 53/60 (88.33%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAV+D  LVAGGFQGELICKHLDRPGVS+CSRTTYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  193 TLAVRDKFLVAGGFQGELICKHLDRPGVSYCSRTTYDDNAITNAIEIYNKPSGALHFTAS 252

HSP 2 Score: 21.557 bits (44), Expect = 3.959e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  249 FTASNNDCGVR 259
[Close]
BLAST of AM162135 vs. TrEMBL
Match: B9HBS7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831736 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.443e-24
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD LL+AGGFQGELICK+LDRPGVSFCSRTTYDDNAITNAIEIY  PSGAVHF AS
Sbjct:  196 TLAVKDRLLIAGGFQGELICKYLDRPGVSFCSRTTYDDNAITNAIEIYECPSGAVHFMAS 255
[Close]
BLAST of AM162135 vs. TrEMBL
Match: D7UAT9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_15.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00014841001 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.435e-23
Identity = 55/60 (91.67%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD LLVAGGFQGELICK+LDRPGVSFCSR TYDDNAITNAIEIYAS SGAVHF AS
Sbjct:  190 TLAVKDKLLVAGGFQGELICKYLDRPGVSFCSRITYDDNAITNAIEIYASSSGAVHFIAS 249
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT1G55680.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr1:20804988-20807294 REVERSE LENGTH=445)

HSP 1 Score: 114.775 bits (286), Expect = 6.870e-27
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAV+D  LVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  193 TLAVRDKFLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYNKPSGALHFTAS 252

HSP 2 Score: 21.557 bits (44), Expect = 6.870e-27
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  249 FTASNNDCGVR 259
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT3G13340.2 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr3:4332370-4334603 FORWARD LENGTH=447)

HSP 1 Score: 114.005 bits (284), Expect = 1.164e-26
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD+ LVAGGFQGELICKHLDRPGVSFCSR TYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  195 TLAVKDDFLVAGGFQGELICKHLDRPGVSFCSRMTYDDNAITNAIEIYNKPSGALHFTAS 254

HSP 2 Score: 21.557 bits (44), Expect = 1.164e-26
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  251 FTASNNDCGVR 261
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT3G13340.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr3:4332370-4334603 FORWARD LENGTH=447)

HSP 1 Score: 114.005 bits (284), Expect = 1.164e-26
Identity = 54/60 (90.00%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVKD+ LVAGGFQGELICKHLDRPGVSFCSR TYDDNAITNAIEIY  PSGA+HFTAS
Sbjct:  195 TLAVKDDFLVAGGFQGELICKHLDRPGVSFCSRMTYDDNAITNAIEIYNKPSGALHFTAS 254

HSP 2 Score: 21.557 bits (44), Expect = 1.164e-26
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  251 FTASNNDCGVR 261
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT5G56190.2 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr5:22742654-22744909 FORWARD LENGTH=447)

HSP 1 Score: 105.531 bits (262), Expect = 3.820e-24
Identity = 49/60 (81.67%), Postives = 55/60 (91.67%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVK+ LLVAGGFQGELICKHLDRPGVSFCSRTTY +NAITNA++IY + SGA+HF AS
Sbjct:  195 TLAVKERLLVAGGFQGELICKHLDRPGVSFCSRTTYTENAITNAVDIYRNSSGALHFMAS 254

HSP 2 Score: 21.557 bits (44), Expect = 3.820e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  251 FMASNNDCGVR 261
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT5G56190.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr5:22742563-22744909 FORWARD LENGTH=441)

HSP 1 Score: 105.531 bits (262), Expect = 3.820e-24
Identity = 49/60 (81.67%), Postives = 55/60 (91.67%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAVHFTAS 180
            TLAVK+ LLVAGGFQGELICKHLDRPGVSFCSRTTY +NAITNA++IY + SGA+HF AS
Sbjct:  189 TLAVKERLLVAGGFQGELICKHLDRPGVSFCSRTTYTENAITNAVDIYRNSSGALHFMAS 248

HSP 2 Score: 21.557 bits (44), Expect = 3.820e-24
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 3 
Query:  168 FHSINNDCGIR 200
            F + NNDCG+R
Sbjct:  245 FMASNNDCGVR 255
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT1G78070.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr1:29355219-29358368 FORWARD LENGTH=449)

HSP 1 Score: 76.6406 bits (187), Expect = 3.337e-15
Identity = 33/55 (60.00%), Postives = 47/55 (85.45%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAV 165
            T+AVKD+L+VAGGFQGELICK ++ P V+FC++ T  DN ITN+++IY +PSG++
Sbjct:  197 TMAVKDDLIVAGGFQGELICKKINEPEVAFCTKLTSADNDITNSVDIYNAPSGSL 251
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT1G36070.2 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr1:13467164-13470386 REVERSE LENGTH=417)

HSP 1 Score: 75.0998 bits (183), Expect = 9.710e-15
Identity = 30/55 (54.55%), Postives = 47/55 (85.45%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAV 165
            ++AVK+NL++ GGF GEL+CK +++PGV+FC+R + +DNAITN ++IY  PSG++
Sbjct:  165 SMAVKENLILLGGFDGELLCKCVNQPGVAFCTRLSTEDNAITNTVDIYRDPSGSL 219
[Close]
BLAST of AM162135 vs. TAIR peptide
Match: AT1G36070.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr1:13467164-13470386 REVERSE LENGTH=418)

HSP 1 Score: 75.0998 bits (183), Expect = 9.710e-15
Identity = 30/55 (54.55%), Postives = 47/55 (85.45%), Query Frame = 1 
Query:    1 TLAVKDNLLVAGGFQGELICKHLDRPGVSFCSRTTYDDNAITNAIEIYASPSGAV 165
            ++AVK+NL++ GGF GEL+CK +++PGV+FC+R + +DNAITN ++IY  PSG++
Sbjct:  166 SMAVKENLILLGGFDGELLCKCVNQPGVAFCTRLSTEDNAITNTVDIYRDPSGSL 220
The following BLAST results are available for this feature:
BLAST of AM162135 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9N5K3_POPTR1.812e-2696.67Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9GHK2_POPTR7.597e-2695.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7L1D2_ARALY6.259e-2591.67WD-40 repeat family protein OS=Arabidopsis lyrata ... [more]
Q9LG00_ARATH1.796e-2490.00F20N2.10 OS=Arabidopsis thaliana PE=4 SV=1[more]
Q9C5N4_ARATH1.796e-2490.00Putative uncharacterized protein At1g55680 OS=Arab... [more]
Q94AB4_ARATH3.042e-2490.00AT3g13340/MDC11_13 OS=Arabidopsis thaliana GN=At3g... [more]
Q9LTT4_ARATH3.045e-2490.00WD domain protein-like OS=Arabidopsis thaliana PE=... [more]
D7KN06_ARALY3.959e-2488.33WD-40 repeat family protein OS=Arabidopsis lyrata ... [more]
B9HBS7_POPTR6.443e-2490.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7UAT9_VITVI1.435e-2391.67Whole genome shotgun sequence of line PN40024, sca... [more]
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BLAST of AM162135 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 8
Match NameE-valueIdentityDescription
AT1G55680.16.870e-2790.00| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT3G13340.21.164e-2690.00| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT3G13340.11.164e-2690.00| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT5G56190.23.820e-2481.67| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT5G56190.13.820e-2481.67| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT1G78070.13.337e-1560.00| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT1G36070.29.710e-1554.55| Symbols: | Transducin/WD40 repeat-like superfam... [more]
AT1G36070.19.710e-1554.55| Symbols: | Transducin/WD40 repeat-like superfam... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162135 ID=AM162135; Name=AM162135; organism=Pisum sativum; type=EST; length=200bp
ACACTTGCAGTAAAAGATAATCTTCTAGTTGCTGGGGGATTTCAGGGTGA
ACTTATTTGCAAGCACCTAGATCGACCTGGTGTCAGCTTTTGCTCAAGGA
CTACTTATGATGATAACGCTATCACCAATGCCATTGAGATTTATGCTTCT
CCTAGTGGGGCAGTTCATTTCACAGCATCAATAATGATTGTGGAATCAGA
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