FG536377

EST Overview


Name	FG536377
Unique Name	FG536377
Type	EST
Organism	Pisum sativum (pea)
Sequence length	408

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Swissprot	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q672H5_PEA (5-alpha-reductase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 3.302e-60
Identity = 112/125 (89.60%), Postives = 112/125 (89.60%), Query Frame = 2

Query:   32 MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITMXXXXXXXXXXXXXGFP 406
            MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITM             GFP
Sbjct:    1 MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITMKTKKKSTLSKTPSGFP 125

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q9FR36_SOYBN (Putative steroid reductase OS=Glycine max PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.305e-40
Identity = 77/99 (77.78%), Postives = 84/99 (84.85%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFIT 355
            D SLF+ +LLTLFLIAPPTF SL FLQAPYGKHHRPGWGPNL PPLAW LMESPTLW TL LFP G +S NPK+  LITPFLIHYFNRTI+YP+ L +T
Sbjct:    8 DSSLFHSSLLTLFLIAPPTFFSLTFLQAPYGKHHRPGWGPNLPPPLAWLLMESPTLWLTLLLFPLGLHSHNPKAQALITPFLIHYFNRTILYPLHLLLT 106

[Close]

BLAST of FG536377 vs. TrEMBL
Match: B9IG44_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_576916 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 6.475e-40
Identity = 71/99 (71.72%), Postives = 88/99 (88.89%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            L D SLF+Y+LL+++LI PPTFISL FLQAPYGKHHRPGWGP +SPPLAW LMESPTLW TL LFP+G + +NPK+IIL++PFL+HYF+RT IYP+R++
Sbjct:    3 LLDQSLFHYSLLSIYLIGPPTFISLKFLQAPYGKHHRPGWGPTISPPLAWILMESPTLWLTLLLFPYGQHFTNPKAIILMSPFLLHYFHRTCIYPLRIY 101

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q8H1T2_GOSHI (Steroid 5-alpha-reductase OS=Gossypium hirsutum GN=DET2 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.034e-37
Identity = 69/96 (71.88%), Postives = 83/96 (86.46%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+Y LLTL++IA PT+ISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T +LFP G +  NPKS +LI+PFL HYFNRT++YP+RL
Sbjct:    4 DQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q2QDF6_GOSHI (Steroid 5-alpha-reductase OS=Gossypium hirsutum GN=DET2 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.034e-37
Identity = 69/96 (71.88%), Postives = 83/96 (86.46%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+Y LLTL++IA PT+ISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T +LFP G +  NPKS +LI+PFL HYFNRT++YP+RL
Sbjct:    4 DQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: B9SPE4_RICCO (3-oxo-5-alpha-steroid 4-dehydrogenase, putative OS=Ricinus communis GN=RCOM_0085180 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 3.008e-37
Identity = 68/97 (70.10%), Postives = 83/97 (85.57%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            D SLF+ +LL L+LI PPTFISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T  LFPFG ++SNPK+ +L+ P+L HYF+RT +YP+RL+
Sbjct:    5 DGSLFHKSLLALYLIGPPTFISLKFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFLLFPFGQHASNPKAWVLMFPYLFHYFHRTCLYPLRLY 101

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q8SA55_PEA (3-oxo-5-alpha-steroid 4-dehydrogenase (Fragment) OS=Pisum sativum GN=det2 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.143e-36
Identity = 69/72 (95.83%), Postives = 71/72 (98.61%), Query Frame = 2

Query:  125 LAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPV 340
            L FLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYF+RTIIYP+
Sbjct:    1 LKFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFHRTIIYPL 72

[Close]

BLAST of FG536377 vs. TrEMBL
Match: D7LKS6_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_669646 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.264e-35
Identity = 63/99 (63.64%), Postives = 82/99 (82.83%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F+Y LLTL    PPT ++L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL +FPFG ++ NPKS++L +P+L+HYF+RTIIYP+RLF
Sbjct:    4 IADQTFFHYCLLTLIFSGPPTAVALKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLIFPFGRHALNPKSLLLFSPYLLHYFHRTIIYPLRLF 102

[Close]

BLAST of FG536377 vs. TrEMBL
Match: D7THB8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034016001 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.183e-33
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+ +L+ L+LIAPPTFISL FLQAPYGKHHR GWGP + P LAWFLMESPT+W TL ++P G+ SSNP+S+ILI+ +L HY +RT+IYP+ L
Sbjct:    4 DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRSGWGPTVPPSLAWFLMESPTVWLTLLIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: A5B1R9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021171 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.183e-33
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+ +L+ L+LIAPPTFISL FLQAPYGKHHR GWGP + P LAWFLMESPT+W TL ++P G+ SSNP+S+ILI+ +L HY +RT+IYP+ L
Sbjct:    4 DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRXGWGPTVPPSLAWFLMESPTVWLTLLIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHL 99

[Close]

BLAST of FG536377 vs. SwissProt
Match: DET2_ARATH (Probable steroid reductase DET2 OS=Arabidopsis thaliana GN=DET2 PE=2 SV=2)

HSP 1 Score: 152.525 bits (384), Expect = 6.218e-37
Identity = 65/99 (65.66%), Postives = 80/99 (80.81%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F Y LLTL    PPT + L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL LFPFG ++ NPKS++L +P+LIHYF+RTIIYP+RLF
Sbjct:    4 IADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLF 102

[Close]

BLAST of FG536377 vs. TAIR peptide
Match: AT2G38050.1 (| Symbols: DET2, DWF6, ATDET2 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | chr2:15921303-15922176 REVERSE LENGTH=262)

HSP 1 Score: 152.525 bits (384), Expect = 7.052e-38
Identity = 65/99 (65.66%), Postives = 80/99 (80.81%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F Y LLTL    PPT + L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL LFPFG ++ NPKS++L +P+LIHYF+RTIIYP+RLF
Sbjct:    4 IADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLF 102

[Close]

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F Y LLTL    PPT + L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL LFPFG ++ NPKS++L +P+LIHYF+RTIIYP+RLF
Sbjct:    4 IADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLF 102

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q672H5_PEA (5-alpha-reductase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 4.129e-60
Identity = 112/125 (89.60%), Postives = 112/125 (89.60%), Query Frame = 2

Query:   32 MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITMXXXXXXXXXXXXXGFP 406
            MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITM             GFP
Sbjct:    1 MLNQKIFLQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFITMKTKKKSTLSKTPSGFP 125

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q9FR36_SOYBN (Putative steroid reductase OS=Glycine max PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.631e-40
Identity = 77/99 (77.78%), Postives = 84/99 (84.85%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFIT 355
            D SLF+ +LLTLFLIAPPTF SL FLQAPYGKHHRPGWGPNL PPLAW LMESPTLW TL LFP G +S NPK+  LITPFLIHYFNRTI+YP+ L +T
Sbjct:    8 DSSLFHSSLLTLFLIAPPTFFSLTFLQAPYGKHHRPGWGPNLPPPLAWLLMESPTLWLTLLLFPLGLHSHNPKAQALITPFLIHYFNRTILYPLHLLLT 106

[Close]

BLAST of FG536377 vs. TrEMBL
Match: B9IG44_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_576916 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 8.096e-40
Identity = 71/99 (71.72%), Postives = 88/99 (88.89%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            L D SLF+Y+LL+++LI PPTFISL FLQAPYGKHHRPGWGP +SPPLAW LMESPTLW TL LFP+G + +NPK+IIL++PFL+HYF+RT IYP+R++
Sbjct:    3 LLDQSLFHYSLLSIYLIGPPTFISLKFLQAPYGKHHRPGWGPTISPPLAWILMESPTLWLTLLLFPYGQHFTNPKAIILMSPFLLHYFHRTCIYPLRIY 101

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q8H1T2_GOSHI (Steroid 5-alpha-reductase OS=Gossypium hirsutum GN=DET2 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.293e-37
Identity = 69/96 (71.88%), Postives = 83/96 (86.46%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+Y LLTL++IA PT+ISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T +LFP G +  NPKS +LI+PFL HYFNRT++YP+RL
Sbjct:    4 DQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q2QDF6_GOSHI (Steroid 5-alpha-reductase OS=Gossypium hirsutum GN=DET2 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.293e-37
Identity = 69/96 (71.88%), Postives = 83/96 (86.46%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+Y LLTL++IA PT+ISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T +LFP G +  NPKS +LI+PFL HYFNRT++YP+RL
Sbjct:    4 DQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: B9SPE4_RICCO (3-oxo-5-alpha-steroid 4-dehydrogenase, putative OS=Ricinus communis GN=RCOM_0085180 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 3.761e-37
Identity = 68/97 (70.10%), Postives = 83/97 (85.57%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            D SLF+ +LL L+LI PPTFISL FLQAPYGKH+RPGWGP +SPPLAWFLMESPTLW T  LFPFG ++SNPK+ +L+ P+L HYF+RT +YP+RL+
Sbjct:    5 DGSLFHKSLLALYLIGPPTFISLKFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFLLFPFGQHASNPKAWVLMFPYLFHYFHRTCLYPLRLY 101

[Close]

BLAST of FG536377 vs. TrEMBL
Match: Q8SA55_PEA (3-oxo-5-alpha-steroid 4-dehydrogenase (Fragment) OS=Pisum sativum GN=det2 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.429e-36
Identity = 69/72 (95.83%), Postives = 71/72 (98.61%), Query Frame = 2

Query:  125 LAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPV 340
            L FLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYF+RTIIYP+
Sbjct:    1 LKFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFHRTIIYPL 72

[Close]

BLAST of FG536377 vs. TrEMBL
Match: D7LKS6_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_669646 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.580e-35
Identity = 63/99 (63.64%), Postives = 82/99 (82.83%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F+Y LLTL    PPT ++L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL +FPFG ++ NPKS++L +P+L+HYF+RTIIYP+RLF
Sbjct:    4 IADQTFFHYCLLTLIFSGPPTAVALKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLIFPFGRHALNPKSLLLFSPYLLHYFHRTIIYPLRLF 102

[Close]

BLAST of FG536377 vs. TrEMBL
Match: D7THB8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034016001 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.479e-33
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+ +L+ L+LIAPPTFISL FLQAPYGKHHR GWGP + P LAWFLMESPT+W TL ++P G+ SSNP+S+ILI+ +L HY +RT+IYP+ L
Sbjct:    4 DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRSGWGPTVPPSLAWFLMESPTVWLTLLIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHL 99

[Close]

BLAST of FG536377 vs. TrEMBL
Match: A5B1R9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021171 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.479e-33
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRL 346
            D +LF+ +L+ L+LIAPPTFISL FLQAPYGKHHR GWGP + P LAWFLMESPT+W TL ++P G+ SSNP+S+ILI+ +L HY +RT+IYP+ L
Sbjct:    4 DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRXGWGPTVPPSLAWFLMESPTVWLTLLIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHL 99

[Close]

BLAST of FG536377 vs. Lotus protein
Match: chr3.CM1961.70.r2.d (+ phase: 0 /pseudo/partial)

HSP 1 Score: 80.8777 bits (198), Expect = 1.597e-16
Identity = 37/50 (74.00%), Postives = 39/50 (78.00%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFL 208
            D  LF+Y LLTL+LIAP TFISL FL A  GKHHR GW P LSPPLAWFL
Sbjct:    8 DKFLFHYVLLTLYLIAPSTFISLRFLHASNGKHHRTGWSPTLSPPLAWFL 57

[Close]

BLAST of FG536377 vs. Lotus protein
Match: CM0457.110.r2.d (+ phase: 0 /partial)

HSP 1 Score: 80.8777 bits (198), Expect = 1.597e-16
Identity = 37/50 (74.00%), Postives = 39/50 (78.00%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFL 208
            D  LF+Y LLTL+LIAP TFISL FL A  GKHHR GW P LSPPLAWFL
Sbjct:    8 DKFLFHYVLLTLYLIAPSTFISLRFLHASNGKHHRTGWSPTLSPPLAWFL 57

[Close]

BLAST of FG536377 vs. Soybean peptides
Match: Glyma07g17410.1|PACid:16267586 ()

HSP 1 Score: 169.859 bits (429), Expect = 6.188e-43
Identity = 77/99 (77.78%), Postives = 85/99 (85.86%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFIT 355
            D SLF+ +LLTLFLIAPPTF+SL FLQAPYGKHHRPGWGPNL PPLAW LMESPTLW TL LFP G +S NPK+  LITPFLIHYFNRTI+YP+ L +T
Sbjct:    8 DSSLFHSSLLTLFLIAPPTFLSLTFLQAPYGKHHRPGWGPNLPPPLAWLLMESPTLWLTLLLFPLGLHSHNPKAQALITPFLIHYFNRTILYPLHLLLT 106

[Close]

BLAST of FG536377 vs. Soybean peptides
Match: Glyma11g01210.1|PACid:16282264 ()

HSP 1 Score: 168.318 bits (425), Expect = 1.801e-42
Identity = 76/99 (76.77%), Postives = 84/99 (84.85%), Query Frame = 2

Query:   59 DDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLFIT 355
            D SLF+ +LLTLFLIAPPTF+SL FLQAPYGKHHRPGWGPNL PPLAW  MESPTLW TL LFP G +S NPK+  LITPFLIHYFNRTI+YP+ L +T
Sbjct:    8 DSSLFHRSLLTLFLIAPPTFLSLTFLQAPYGKHHRPGWGPNLPPPLAWLFMESPTLWLTLLLFPLGLHSHNPKAQALITPFLIHYFNRTILYPLHLLLT 106

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BLAST of FG536377 vs. SwissProt
Match: DET2_ARATH (Probable steroid reductase DET2 OS=Arabidopsis thaliana GN=DET2 PE=2 SV=2)

HSP 1 Score: 152.525 bits (384), Expect = 6.330e-37
Identity = 65/99 (65.66%), Postives = 80/99 (80.81%), Query Frame = 2

Query:   53 LQDDSLFNYALLTLFLIAPPTFISLAFLQAPYGKHHRPGWGPNLSPPLAWFLMESPTLWFTLYLFPFGNNSSNPKSIILITPFLIHYFNRTIIYPVRLF 349
            + D + F Y LLTL    PPT + L FLQAPYGKH+R GWGP +SPP+AWF+MESPTLW TL LFPFG ++ NPKS++L +P+LIHYF+RTIIYP+RLF
Sbjct:    4 IADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLF 102

The following BLAST results are available for this feature:

BLAST of FG536377 vs. TrEMBL
BLAST of FG536377 vs. SwissProt
BLAST of FG536377 vs. TAIR peptide
BLAST of FG536377 vs. TAIR peptide
BLAST of FG536377 vs. TrEMBL
BLAST of FG536377 vs. Lotus protein
BLAST of FG536377 vs. Soybean peptides
BLAST of FG536377 vs. SwissProt

BLAST of FG536377 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q672H5_PEA	3.302e-60	89.60	5-alpha-reductase OS=Pisum sativum PE=2 SV=1	[more]
Q9FR36_SOYBN	1.305e-40	77.78	Putative steroid reductase OS=Glycine max PE=4 SV=...	[more]
B9IG44_POPTR	6.475e-40	71.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8H1T2_GOSHI	1.034e-37	71.88	Steroid 5-alpha-reductase OS=Gossypium hirsutum GN...	[more]
Q2QDF6_GOSHI	1.034e-37	71.88	Steroid 5-alpha-reductase OS=Gossypium hirsutum GN...	[more]
B9SPE4_RICCO	3.008e-37	70.10	3-oxo-5-alpha-steroid 4-dehydrogenase, putative OS...	[more]
Q8SA55_PEA	1.143e-36	95.83	3-oxo-5-alpha-steroid 4-dehydrogenase (Fragment) O...	[more]
D7LKS6_ARALY	1.264e-35	63.64	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
D7THB8_VITVI	1.183e-33	65.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B1R9_VITVI	1.183e-33	65.63	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of FG536377 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
DET2_ARATH	6.218e-37	65.66	Probable steroid reductase DET2 OS=Arabidopsis tha...	[more]

BLAST of FG536377 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G38050.1	7.052e-38	65.66	\| Symbols: DET2, DWF6, ATDET2 \| 3-oxo-5-alpha-ster...	[more]

BLAST of FG536377 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G38050.1	7.052e-38	65.66	\| Symbols: DET2, DWF6, ATDET2 \| 3-oxo-5-alpha-ster...	[more]

BLAST of FG536377 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q672H5_PEA	4.129e-60	89.60	5-alpha-reductase OS=Pisum sativum PE=2 SV=1	[more]
Q9FR36_SOYBN	1.631e-40	77.78	Putative steroid reductase OS=Glycine max PE=4 SV=...	[more]
B9IG44_POPTR	8.096e-40	71.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8H1T2_GOSHI	1.293e-37	71.88	Steroid 5-alpha-reductase OS=Gossypium hirsutum GN...	[more]
Q2QDF6_GOSHI	1.293e-37	71.88	Steroid 5-alpha-reductase OS=Gossypium hirsutum GN...	[more]
B9SPE4_RICCO	3.761e-37	70.10	3-oxo-5-alpha-steroid 4-dehydrogenase, putative OS...	[more]
Q8SA55_PEA	1.429e-36	95.83	3-oxo-5-alpha-steroid 4-dehydrogenase (Fragment) O...	[more]
D7LKS6_ARALY	1.580e-35	63.64	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
D7THB8_VITVI	1.479e-33	65.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5B1R9_VITVI	1.479e-33	65.63	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of FG536377 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 2


Match Name	E-value	Identity	Description
chr3.CM1961.70.r2.d	1.597e-16	74.00	+ phase: 0 /pseudo/partial	[more]
CM0457.110.r2.d	1.597e-16	74.00	+ phase: 0 /partial	[more]

BLAST of FG536377 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2


Match Name	E-value	Identity	Description
Glyma07g17410.1\|PACid:16267586	6.188e-43	77.78		[more]
Glyma11g01210.1\|PACid:16282264	1.801e-42	76.77		[more]

BLAST of FG536377 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
DET2_ARATH	6.330e-37	65.66	Probable steroid reductase DET2 OS=Arabidopsis tha...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 50..122 score: 5.9
None	No IPR available	PANTHER	PTHR10556:SF3	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 50..122 score: 5.9
None	No IPR available	SEG	seg	seg	coord: 116..128 score
None	No IPR available	SEG	seg	seg	coord: 50..66 score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 50..122 score: 5.9
None	No IPR available	PANTHER	PTHR10556:SF3	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 50..122 score: 5.9
None	No IPR available	SEG	seg	seg	coord: 116..128 score
None	No IPR available	SEG	seg	seg	coord: 50..66 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG536377 ID=FG536377; Name=FG536377; organism=Pisum sativum; type=EST; length=408bp
CGCACAAACATAGTACCTAACACGCACAACAATGTTGAATCAAAAGATAT
TCTTACAAGACGATTCGCTCTTCAACTACGCACTCCTCACTCTCTTCCTC
ATAGCACCACCAACCTTCATCTCTCTCGCATTCCTCCAAGCTCCATATGG
CAAACACCACAGACCAGGTTGGGGTCCAAATCTCTCTCCACCTCTCGCAT
GGTTTCTCATGGAAAGTCCCACACTTTGGTTCACCCTTTATCTCTTCCCA
TTCGGTAACAACTCTTCAAACCCTAAATCAATTATCCTCATCACCCCCTT
TCTAATTCATTACTTCAACCGCACAATCATCTACCCAGTTCGTTTGTTCA
TCACAATGAAAACGAAAAAGAAGTCAACTTTATCGAAAACCCCATCTGGG
TTTCCTTT

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