FG531914

EST Overview


Name	FG531914
Unique Name	FG531914
Type	EST
Organism	Pisum sativum (pea)
Sequence length	334

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG531914 vs. TrEMBL
Match: C6T403_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 2.728e-38
Identity = 72/81 (88.89%), Postives = 75/81 (92.59%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E+KLTQLGVRQW KWGCPPSKFPWTY+SKETCYLLEGKVKVTP+GANESVE  AGD V FPKGMSCTWDVSVAVDKHY FE
Sbjct:   18 EDKLTQLGVRQWPKWGCPPSKFPWTYESKETCYLLEGKVKVTPSGANESVEIAAGDFVEFPKGMSCTWDVSVAVDKHYNFE 98

[Close]

BLAST of FG531914 vs. TrEMBL
Match: C6T4F2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 3.017e-37
Identity = 70/81 (86.42%), Postives = 74/81 (91.36%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVRQW KWGCPPSKFPWTY++KETCYLLEGKVKV P+G+NESVE  AGDLVVFPKGMSCTWDVSV VDKHY FE
Sbjct:   62 ESKLTQLGVRQWPKWGCPPSKFPWTYEAKETCYLLEGKVKVFPSGSNESVEIAAGDLVVFPKGMSCTWDVSVGVDKHYNFE 142

[Close]

BLAST of FG531914 vs. TrEMBL
Match: C6SYR7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 3.017e-37
Identity = 70/81 (86.42%), Postives = 74/81 (91.36%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVRQW KWGCPPSKFPWTY++KETCYLLEGKVKV P+G+NESVE  AGDLVVFPKGMSCTWDVSV VDKHY FE
Sbjct:   62 ESKLTQLGVRQWPKWGCPPSKFPWTYEAKETCYLLEGKVKVFPSGSNESVEIAAGDLVVFPKGMSCTWDVSVGVDKHYNFE 142

[Close]

BLAST of FG531914 vs. TrEMBL
Match: Q9SV91_ARATH (At4g10300 OS=Arabidopsis thaliana GN=F24G24.100 PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.162e-35
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVR W KWGCPPSKFPWTY +KETCYLL+GKVKV PNG++E VE  AGD VVFPKGMSCTWDVSVAVDKHY FE
Sbjct:   54 ESKLTQLGVRSWPKWGCPPSKFPWTYSAKETCYLLQGKVKVYPNGSDEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 134

[Close]

BLAST of FG531914 vs. TrEMBL
Match: Q8LEK1_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.162e-35
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVR W KWGCPPSKFPWTY +KETCYLL+GKVKV PNG++E VE  AGD VVFPKGMSCTWDVSVAVDKHY FE
Sbjct:   56 ESKLTQLGVRSWPKWGCPPSKFPWTYSAKETCYLLQGKVKVYPNGSDEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 136

[Close]

BLAST of FG531914 vs. TrEMBL
Match: D7LY36_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489873 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.162e-35
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVR W +WGCPPSKFPWTY +KETCYLLEGKVKV PNG++E VE  AGD VVFPKGMSCTWDVSVAVDKHY FE
Sbjct:   56 ESKLTQLGVRNWHRWGCPPSKFPWTYSAKETCYLLEGKVKVYPNGSDEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 136

[Close]

BLAST of FG531914 vs. TrEMBL
Match: B9S1T1_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0867910 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 6.290e-35
Identity = 66/81 (81.48%), Postives = 69/81 (85.19%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLT LGVR W KWGCPPSKFPWTY +KETCYLLEGKVKV P+G  E +E GAGDLVVFPKGMSCTWDVSV VDKHY FE
Sbjct:   26 ESKLTDLGVRSWPKWGCPPSKFPWTYSAKETCYLLEGKVKVYPDGIEEPIEIGAGDLVVFPKGMSCTWDVSVGVDKHYNFE 106

[Close]

BLAST of FG531914 vs. TrEMBL
Match: A9TEC3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_144325 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.122e-34
Identity = 63/82 (76.83%), Postives = 72/82 (87.80%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            SE +LT+LG+R W KWGCPPSKFPWTYD+ ETC+LL+GKVKV P G++E VEFGAGDLVVFPKGMSCTWDVS  VDKHY F+
Sbjct:   24 SESRLTELGIRSWPKWGCPPSKFPWTYDATETCFLLQGKVKVYPEGSSEFVEFGAGDLVVFPKGMSCTWDVSETVDKHYQFD 105

[Close]

BLAST of FG531914 vs. TrEMBL
Match: A9PG71_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 5.325e-34
Identity = 65/81 (80.25%), Postives = 70/81 (86.42%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLT+LGVR+W KWGCPPSKFPWTY +KETCYLLEGKVKV P+GA E VE  AGDLV FPKGMSCTWDV+V VDKHY FE
Sbjct:   82 ESKLTELGVRKWPKWGCPPSKFPWTYSAKETCYLLEGKVKVYPDGAEEPVEITAGDLVEFPKGMSCTWDVAVGVDKHYNFE 162

[Close]

BLAST of FG531914 vs. TrEMBL
Match: D7SMT5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_89.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00037105001 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 4.508e-33
Identity = 63/78 (80.77%), Postives = 69/78 (88.46%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHY 237
            E +LTQLGV+ W KWGC PSKFPWT+ +KETCYLL+GKVKV P+GANE VE GAGDLV FPKGMSCTWDVSVAVDKHY
Sbjct:   83 ESRLTQLGVKSWPKWGCEPSKFPWTFTTKETCYLLKGKVKVYPDGANECVEIGAGDLVEFPKGMSCTWDVSVAVDKHY 160

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT4G10300.1 (| Symbols: | RmlC-like cupins superfamily protein | chr4:6384564-6385946 FORWARD LENGTH=134)

HSP 1 Score: 151.754 bits (382), Expect = 8.334e-38
Identity = 67/81 (82.72%), Postives = 71/81 (87.65%), Query Frame = 1

Query:    4 EEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            E KLTQLGVR W KWGCPPSKFPWTY +KETCYLL+GKVKV PNG++E VE  AGD VVFPKGMSCTWDVSVAVDKHY FE
Sbjct:   54 ESKLTQLGVRSWPKWGCPPSKFPWTYSAKETCYLLQGKVKVYPNGSDEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 134

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT3G04300.1 (| Symbols: | RmlC-like cupins superfamily protein | chr3:1140318-1140723 FORWARD LENGTH=96)

HSP 1 Score: 116.316 bits (290), Expect = 3.880e-27
Identity = 48/82 (58.54%), Postives = 62/82 (75.61%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S  +L+ LGV  W KW C P K+   ++ +ETCYL++GKVKV P G++E VEFGAGDLV  PKG+SCTWDVS+ +DKHY F+
Sbjct:   10 SSRRLSDLGVMSWPKWSCQPGKYALVFEERETCYLVKGKVKVYPKGSSEFVEFGAGDLVTIPKGLSCTWDVSLFIDKHYKFD 91

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT4G28703.1 (| Symbols: | RmlC-like cupins superfamily protein | chr4:14166365-14166798 FORWARD LENGTH=107)

HSP 1 Score: 106.301 bits (264), Expect = 4.016e-24
Identity = 48/90 (53.33%), Postives = 61/90 (67.78%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANES---------VEFGAGDLVVFPKGMSCTWDVSVAVDKHYIF 243
            S+ +L +L  + W KWGC P K+   Y+++E CY+L GKVKV P     S         VEFGAGD+V FPKG+SCTWDVS++VDKHYIF
Sbjct:   15 SQARLDELKFKSWPKWGCSPGKYHLKYEAEEICYILRGKVKVYPKPPPSSSSDAEVEWCVEFGAGDIVTFPKGLSCTWDVSLSVDKHYIF 104

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT4G10280.1 (| Symbols: | RmlC-like cupins superfamily protein | chr4:6375220-6375996 REVERSE LENGTH=140)

HSP 1 Score: 90.5077 bits (223), Expect = 2.280e-19
Identity = 43/85 (50.59%), Postives = 52/85 (61.18%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNG---ANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S+ KL QLGV  W KW   PSKFPW +   ET Y +EGKVKV  +G     E+ E G GD+VVFPK M   W+++ AV K Y  E
Sbjct:   55 SDTKLAQLGVASWPKWEGAPSKFPWEFKKTETIYFMEGKVKVNVDGYDEEEETFEIGKGDVVVFPKDMKVVWEITEAVKKQYSLE 139

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT4G10290.1 (| Symbols: | RmlC-like cupins superfamily protein | chr4:6379958-6381720 REVERSE LENGTH=120)

HSP 1 Score: 73.559 bits (179), Expect = 2.884e-14
Identity = 39/85 (45.88%), Postives = 49/85 (57.65%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKV---TPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S+ KL +LGV  W  W   P KFPW +   ET Y +EGK+KV     +   E++EF AGDLVVFP+ M+   DV   V K Y  E
Sbjct:   27 SDAKLAELGVTSWQLWESMPRKFPWKFKKTETMYFVEGKLKVKVEDHHKEGEALEFVAGDLVVFPQDMNVFVDVIEDVKKRYYRE 111

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT2G32650.2 (| Symbols: | RmlC-like cupins superfamily protein | chr2:13851547-13851966 FORWARD LENGTH=139)

HSP 1 Score: 61.2326 bits (147), Expect = 1.481e-10
Identity = 27/82 (32.93%), Postives = 46/82 (56.10%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S+++L +LGV +WS W     K PW +   +  Y+ +G+V+V P G+   ++F AGDLV +PK +      +    + Y F+
Sbjct:   53 SQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVRVVPEGSKRYMQFLAGDLVRYPKWLEADLFFNAPYIERYCFK 134

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT2G32180.1 (| Symbols: PTAC18 | plastid transcriptionally active 18 | chr2:13672362-13672781 REVERSE LENGTH=139)

HSP 1 Score: 61.2326 bits (147), Expect = 1.481e-10
Identity = 27/82 (32.93%), Postives = 46/82 (56.10%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S+++L +LGV +WS W     K PW +   +  Y+ +G+V+V P G+   ++F AGDLV +PK +      +    + Y F+
Sbjct:   53 SQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVRVVPEGSKRYMQFLAGDLVRYPKWLEADLFFNAPYIERYCFK 134

[Close]

BLAST of FG531914 vs. TAIR peptide
Match: AT2G32650.1 (| Symbols: | RmlC-like cupins superfamily protein | chr2:13851547-13851966 FORWARD LENGTH=139)

HSP 1 Score: 61.2326 bits (147), Expect = 1.481e-10
Identity = 27/82 (32.93%), Postives = 46/82 (56.10%), Query Frame = 1

Query:    1 SEEKLTQLGVRQWSKWGCPPSKFPWTYDSKETCYLLEGKVKVTPNGANESVEFGAGDLVVFPKGMSCTWDVSVAVDKHYIFE 246
            S+++L +LGV +WS W     K PW +   +  Y+ +G+V+V P G+   ++F AGDLV +PK +      +    + Y F+
Sbjct:   53 SQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVRVVPEGSKRYMQFLAGDLVRYPKWLEADLFFNAPYIERYCFK 134

The following BLAST results are available for this feature:

BLAST of FG531914 vs. TrEMBL
BLAST of FG531914 vs. TAIR peptide

BLAST of FG531914 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T403_SOYBN	2.728e-38	88.89	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T4F2_SOYBN	3.017e-37	86.42	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SYR7_SOYBN	3.017e-37	86.42	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q9SV91_ARATH	2.162e-35	82.72	At4g10300 OS=Arabidopsis thaliana GN=F24G24.100 PE...	[more]
Q8LEK1_ARATH	2.162e-35	82.72	Putative uncharacterized protein OS=Arabidopsis th...	[more]
D7LY36_ARALY	2.162e-35	82.72	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9S1T1_RICCO	6.290e-35	81.48	Putative uncharacterized protein OS=Ricinus commun...	[more]
A9TEC3_PHYPA	3.122e-34	76.83	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9PG71_POPTR	5.325e-34	80.25	Putative uncharacterized protein OS=Populus tricho...	[more]
D7SMT5_VITVI	4.508e-33	80.77	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of FG531914 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 8


Match Name	E-value	Identity	Description
AT4G10300.1	8.334e-38	82.72	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT3G04300.1	3.880e-27	58.54	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT4G28703.1	4.016e-24	53.33	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT4G10280.1	2.280e-19	50.59	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT4G10290.1	2.884e-14	45.88	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT2G32650.2	1.481e-10	32.93	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]
AT2G32180.1	1.481e-10	32.93	\| Symbols: PTAC18 \| plastid transcriptionally acti...	[more]
AT2G32650.1	1.481e-10	32.93	\| Symbols: \| RmlC-like cupins superfamily protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008579	Domain of unknown function DUF861, cupin-3	PFAM	PF05899	Cupin_3	coord: 6..79 score: 3.1
IPR011051	Cupin, RmlC-type	SUPERFAMILY	51182	RmlC-like cupins	coord: 5..81 score: 4.3
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	no description	coord: 7..81 score: 6.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531914 ID=FG531914; Name=FG531914; organism=Pisum sativum; type=EST; length=334bp
TCAGAGGAAAAACTCACTCAACTCGGTGTTAGGCAATGGTCCAAATGGGG
CTGTCCTCCAAGCAAATTCCCATGGACTTATGATTCAAAAGAGACATGCT
ATCTCTTAGAAGGAAAAGTTAAGGTAACACCAAATGGAGCAAATGAGTCG
GTTGAATTTGGTGCTGGTGATTTGGTTGTGTTTCCTAAAGGCATGAGTTG
TACTTGGGATGTTTCTGTTGCTGTCGACAAGCACTATATCTTTGAATAAC
TCTTAAATGCCGCAACAACCGAAATGGAGGACTTTCATGAAATTATTTGG
CATTAAGATTTTTAAGTGNAATATGAACTTTGAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR008579	UGlyAH_Cupin_dom
IPR011051	RmlC_Cupin_sf
IPR014710	RmlC-like_jellyroll

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