FG532098

EST Overview


Name	FG532098
Unique Name	FG532098
Type	EST
Organism	Pisum sativum (pea)
Sequence length	312

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG532098 vs. TrEMBL
Match: D7U9W2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031153001 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.128e-18
Identity = 46/66 (69.70%), Postives = 53/66 (80.30%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            YKT VLKVSIHC+GCKKKV KILQ+I GV +  ID +QQKV VTGNV+ + L+ KL K GKHAELW
Sbjct:   20 YKTWVLKVSIHCEGCKKKVKKILQNIDGVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELW 85

[Close]

BLAST of FG532098 vs. TrEMBL
Match: A5AP55_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004114 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.128e-18
Identity = 46/66 (69.70%), Postives = 53/66 (80.30%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            YKT VLKVSIHC+GCKKKV KILQ+I GV +  ID +QQKV VTGNV+ + L+ KL K GKHAELW
Sbjct:   20 YKTWVLKVSIHCEGCKKKVKKILQNIDGVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELW 85

[Close]

BLAST of FG532098 vs. TrEMBL
Match: B9HSB6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_768813 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.553e-17
Identity = 44/65 (67.69%), Postives = 53/65 (81.54%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            KT VLKVSIHCQGCK+KV K+LQSI GV + +ID +QQ+V VTGN+ +  LI KL KTGKHAE+W
Sbjct:   17 KTWVLKVSIHCQGCKRKVRKVLQSIDGVFTTSIDSQQQRVTVTGNIEAGTLIKKLMKTGKHAEIW 81

[Close]

BLAST of FG532098 vs. TrEMBL
Match: Q5WN03_ORYSJ (Os05g0150700 protein OS=Oryza sativa subsp. japonica GN=P0001A07.6 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.028e-17
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T+ LKVSIHC+GCKKKV K+L SI GV   +ID++ QKVVV GNV+ D L+ KL KTGKHAE W
Sbjct:   13 YQTLALKVSIHCEGCKKKVKKVLHSIEGVYKTDIDVQHQKVVVIGNVSVDTLVKKLVKTGKHAEPW 78

[Close]

BLAST of FG532098 vs. TrEMBL
Match: B9FHD9_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_17143 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.028e-17
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T+ LKVSIHC+GCKKKV K+L SI GV   +ID++ QKVVV GNV+ D L+ KL KTGKHAE W
Sbjct:   13 YQTLALKVSIHCEGCKKKVKKVLHSIEGVYKTDIDVQHQKVVVIGNVSVDTLVKKLVKTGKHAEPW 78

[Close]

BLAST of FG532098 vs. TrEMBL
Match: A2Y0G6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18480 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.028e-17
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T+ LKVSIHC+GCKKKV K+L SI GV   +ID++ QKVVV GNV+ D L+ KL KTGKHAE W
Sbjct:   13 YQTLALKVSIHCEGCKKKVKKVLHSIEGVYKTDIDVQHQKVVVIGNVSVDTLVKKLVKTGKHAEPW 78

[Close]

BLAST of FG532098 vs. TrEMBL
Match: B9RFA4_RICCO (Chloroplast-targeted copper chaperone, putative OS=Ricinus communis GN=RCOM_1433240 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.648e-17
Identity = 45/66 (68.18%), Postives = 51/66 (77.27%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            YKT VLKVSIHC+GCK+KV KIL +I GV +  IDL+QQKV V GNV+   LI KL K GKHAELW
Sbjct:   35 YKTWVLKVSIHCEGCKRKVKKILTNIDGVYATEIDLRQQKVTVIGNVDGGTLIKKLVKAGKHAELW 100

[Close]

BLAST of FG532098 vs. TrEMBL
Match: D7U255_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027966001 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.459e-17
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T VLKVSIHC+GCKKKV K+L SI GV +  ID +Q KV VTGNV+++ LI KL KTGKHA+LW
Sbjct:   15 YQTWVLKVSIHCEGCKKKVKKVLHSIDGVYTTVIDSQQHKVTVTGNVDAETLIKKLVKTGKHADLW 80

[Close]

BLAST of FG532098 vs. TrEMBL
Match: C0P480_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.459e-17
Identity = 42/66 (63.64%), Postives = 53/66 (80.30%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T+ L+VSIHC+GCKKKV K+L SI GV   +ID +QQKVVV GNV++D L+ KL K+GKHAE W
Sbjct:   13 YQTLALRVSIHCEGCKKKVKKVLHSIEGVYKTDIDTQQQKVVVIGNVSADALVKKLLKSGKHAEPW 78

[Close]

BLAST of FG532098 vs. TrEMBL
Match: B6T3X5_MAIZE (Pro-resilin OS=Zea mays PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.459e-17
Identity = 42/66 (63.64%), Postives = 53/66 (80.30%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y+T+ L+VSIHC+GCKKKV K+L SI GV   +ID +QQKVVV GNV++D L+ KL K+GKHAE W
Sbjct:   13 YQTLALRVSIHCEGCKKKVKKVLHSIEGVYKTDIDTQQQKVVVIGNVSADALVKKLLKSGKHAEPW 78

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT5G27690.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr5:9803796-9805036 FORWARD LENGTH=352)

HSP 1 Score: 90.1225 bits (222), Expect = 2.943e-19
Identity = 42/66 (63.64%), Postives = 51/66 (77.27%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            Y T VL+VSIHC+GCK+K+ KIL  I GV + NID+KQQKV V GNV  +ILI K+ K G+HAELW
Sbjct:   29 YTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAELW 94

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT3G06130.2 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr3:1853193-1854673 REVERSE LENGTH=349)

HSP 1 Score: 82.8037 bits (203), Expect = 4.699e-17
Identity = 39/65 (60.00%), Postives = 50/65 (76.92%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T VLKV+IHC GCK+KV KILQ I GV +  ID +Q KV V+G+V+  +LI KL K+GKHAE+W
Sbjct:   10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGKHAEIW 74

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT3G06130.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr3:1853048-1854673 REVERSE LENGTH=473)

HSP 1 Score: 82.8037 bits (203), Expect = 4.699e-17
Identity = 39/65 (60.00%), Postives = 50/65 (76.92%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T VLKV+IHC GCK+KV KILQ I GV +  ID +Q KV V+G+V+  +LI KL K+GKHAE+W
Sbjct:   10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGKHAEIW 74

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT5G19090.3 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr5:6387910-6389855 FORWARD LENGTH=465)

HSP 1 Score: 82.0333 bits (201), Expect = 8.015e-17
Identity = 39/65 (60.00%), Postives = 49/65 (75.38%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T VLKV+IHC GCK+KV KILQ I GV +  ID +  KV V+GNV+  +LI KL K+GKHAE+W
Sbjct:   10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIW 74

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT5G19090.2 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr5:6387910-6389855 FORWARD LENGTH=465)

HSP 1 Score: 82.0333 bits (201), Expect = 8.015e-17
Identity = 39/65 (60.00%), Postives = 49/65 (75.38%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T VLKV+IHC GCK+KV KILQ I GV +  ID +  KV V+GNV+  +LI KL K+GKHAE+W
Sbjct:   10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIW 74

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT5G19090.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr5:6387910-6389855 FORWARD LENGTH=587)

HSP 1 Score: 82.0333 bits (201), Expect = 8.015e-17
Identity = 39/65 (60.00%), Postives = 49/65 (75.38%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T VLKV+IHC GCK+KV KILQ I GV +  ID +  KV V+GNV+  +LI KL K+GKHAE+W
Sbjct:   10 QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIW 74

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT1G56210.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr1:21035715-21037019 FORWARD LENGTH=364)

HSP 1 Score: 80.1073 bits (196), Expect = 3.046e-16
Identity = 39/64 (60.94%), Postives = 49/64 (76.56%), Query Frame = 2

Query:   14 YKTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAE 205
            YK+  LKVSIHC+GCK+KV KIL SI GV  V+ID+KQ KV V G ++ +IL+ KL K GK+AE
Sbjct:   39 YKSCTLKVSIHCEGCKRKVKKILTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAE 102

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT1G23000.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr1:8143614-8144865 FORWARD LENGTH=358)

HSP 1 Score: 79.337 bits (194), Expect = 5.195e-16
Identity = 38/65 (58.46%), Postives = 48/65 (73.85%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAELW 211
            +T  L+V+IHC+GC KKV K+LQ I GV  V I+ + QKV V+G+V+S  LI KL K GKHAELW
Sbjct:   13 QTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDSATLINKLVKAGKHAELW 77

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT3G05220.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr3:1488808-1491171 FORWARD LENGTH=577)

HSP 1 Score: 78.1814 bits (191), Expect = 1.157e-15
Identity = 36/64 (56.25%), Postives = 50/64 (78.12%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAEL 208
            +T VLKV++HC+GCK KV K LQ I GV SV  D++Q +V VTGN++  +L+ KL+K+GKHAE+
Sbjct:   10 QTCVLKVNVHCEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGNIDPALLVKKLSKSGKHAEI 73

[Close]

BLAST of FG532098 vs. TAIR peptide
Match: AT5G37860.1 (| Symbols: | Heavy metal transport/detoxification superfamily protein | chr5:15069578-15070555 REVERSE LENGTH=262)

HSP 1 Score: 75.8702 bits (185), Expect = 5.744e-15
Identity = 37/64 (57.81%), Postives = 48/64 (75.00%), Query Frame = 2

Query:   17 KTIVLKVSIHCQGCKKKVIKILQSIYGVTSVNIDLKQQKVVVTGNVNSDILIMKLTKTGKHAEL 208
            KT  LKV+I+CQGCK KV K L+ I GV SV+ID  Q+ V+V GN++ +IL+ KL K GKHA+L
Sbjct:   10 KTYFLKVNINCQGCKMKVKKTLRKIEGVYSVDIDTDQEAVIVRGNLDPEILVKKLNKRGKHAQL 73

The following BLAST results are available for this feature:

BLAST of FG532098 vs. TrEMBL
BLAST of FG532098 vs. TAIR peptide

BLAST of FG532098 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7U9W2_VITVI	3.128e-18	69.70	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AP55_VITVI	3.128e-18	69.70	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9HSB6_POPTR	1.553e-17	67.69	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q5WN03_ORYSJ	2.028e-17	65.15	Os05g0150700 protein OS=Oryza sativa subsp. japoni...	[more]
B9FHD9_ORYSJ	2.028e-17	65.15	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2Y0G6_ORYSI	2.028e-17	65.15	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B9RFA4_RICCO	2.648e-17	68.18	Chloroplast-targeted copper chaperone, putative OS...	[more]
D7U255_VITVI	3.459e-17	66.67	Whole genome shotgun sequence of line PN40024, sca...	[more]
C0P480_MAIZE	3.459e-17	63.64	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6T3X5_MAIZE	3.459e-17	63.64	Pro-resilin OS=Zea mays PE=2 SV=1	[more]

BLAST of FG532098 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G27690.1	2.943e-19	63.64	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT3G06130.2	4.699e-17	60.00	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT3G06130.1	4.699e-17	60.00	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT5G19090.3	8.015e-17	60.00	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT5G19090.2	8.015e-17	60.00	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT5G19090.1	8.015e-17	60.00	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT1G56210.1	3.046e-16	60.94	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT1G23000.1	5.195e-16	58.46	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT3G05220.1	1.157e-15	56.25	\| Symbols: \| Heavy metal transport/detoxification...	[more]
AT5G37860.1	5.744e-15	57.81	\| Symbols: \| Heavy metal transport/detoxification...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal transport/detoxification protein	PFAM	PF00403	HMA	coord: 10..65 score: 1.7
IPR006121	Heavy metal transport/detoxification protein	PROFILE	PS50846	HMA_2	coord: 6..69 score: 10
IPR006121	Heavy metal transport/detoxification protein	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 1..72 score: 4.6
None	No IPR available	GENE3D	3.30.70.100	no description	coord: 5..72 score: 7.4
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 13..91 score: 3.2
None	No IPR available	PANTHER	PTHR22814:SF16	CHLOROPLAST-TARGETED COPPER CHAPERONE	coord: 13..91 score: 3.2
None	No IPR available	SEG	seg	seg	coord: 71..99 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG532098 ID=FG532098; Name=FG532098; organism=Pisum sativum; type=EST; length=312bp
TACACATCCAAACTACAAGACGATTGTCTTGAAGGTCTCCATTCACTGCC
AAGGCTGCAAGAAAAAAGTCATCAAAATTCTTCAAAGCATATATGGTGTT
ACTAGCGTAAACATTGATTTGAAGCAACAAAAAGTTGTAGTAACTGGGAA
CGTAAACAGCGATATTTTGATCATGAAATTGACCAAGACAGGGAAGCATG
CTGAGTTATGGCCTCAACCTGAAACGGAACCTGAACCAAAACCAACCGAT
GCTAAAAAGAAGAAGCAGAGAAAACCTGAGAGCAAAGAGAAACAGAGCGA
TCCAGAAACTGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

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