FG532313

EST Overview


Name	FG532313
Unique Name	FG532313
Type	EST
Organism	Pisum sativum (pea)
Sequence length	200

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG532313 vs. TrEMBL
Match: A5BMX1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005315 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.777e-24
Identity = 56/65 (86.15%), Postives = 59/65 (90.77%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYI+REYISNSEERGVD  DLISF+LELSFHV  EAYNINTLT+FQRN IKDL DLGLVKLQ
Sbjct:  203 AQLWYIMREYISNSEERGVDPADLISFLLELSFHVTGEAYNINTLTEFQRNTIKDLVDLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: B9RZ76_RICCO (Tfiih, polypeptide, putative OS=Ricinus communis GN=RCOM_0936010 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.124e-23
Identity = 53/65 (81.54%), Postives = 60/65 (92.31%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYISNSEERG+D+ DLISF+LELSFH+  EAYN+  LT+FQRN+IKDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYISNSEERGLDSADLISFLLELSFHITGEAYNMIMLTEFQRNMIKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: B9H2W7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_817868 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.535e-22
Identity = 52/65 (80.00%), Postives = 58/65 (89.23%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI+NSEERG +  DLISF+LELSFHV  EAYN+NTLT+ QRN IKDLA+LGLVKLQ
Sbjct:  201 AQLWYIIREYITNSEERGTEPADLISFLLELSFHVTGEAYNMNTLTEIQRNTIKDLAELGLVKLQ 265

[Close]

BLAST of FG532313 vs. TrEMBL
Match: D7MC99_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493174 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.105e-21
Identity = 51/65 (78.46%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYISN+EER V+  DLISF+LELSFHV  EAYN NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYISNAEERDVEPADLISFLLELSFHVTGEAYNSNTLTEVQNNTLKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: Q7XKD9_ORYSJ (OSJNBb0017I01.15 protein OS=Oryza sativa subsp. japonica GN=OSJNBb0017I01.15 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 49/65 (75.38%), Postives = 59/65 (90.77%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYI+REYIS++EERGVD  +LISF+LELSFH + EAY++NTLTD QRN I+DLA+LGLVKLQ
Sbjct:  203 AQLWYIMREYISSAEERGVDPTELISFLLELSFHTLGEAYSLNTLTDVQRNAIRDLAELGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: Q680U9_ARATH (At4g17020 OS=Arabidopsis thaliana GN=At4g17020 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI N+EER VD  DLISF+LELSFHV  +AYN+NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYILNAEERDVDPADLISFLLELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: Q680F9_ARATH (MRNA, complete cds, clone: RAFL22-56-M10 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI N+EER VD  DLISF+LELSFHV  +AYN+NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYILNAEERDVDPADLISFLLELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TrEMBL
Match: Q259V6_ORYSA (B0213E10.4 protein OS=Oryza sativa GN=B0213E10.4 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 49/65 (75.38%), Postives = 59/65 (90.77%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYI+REYIS++EERGVD  +LISF+LELSFH + EAY++NTLTD QRN I+DLA+LGLVKLQ
Sbjct:  250 AQLWYIMREYISSAEERGVDPTELISFLLELSFHTLGEAYSLNTLTDVQRNAIRDLAELGLVKLQ 314

[Close]

BLAST of FG532313 vs. TrEMBL
Match: Q0J8Y9_ORYSJ (Os04g0680000 protein OS=Oryza sativa subsp. japonica GN=Os04g0680000 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 49/65 (75.38%), Postives = 59/65 (90.77%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYI+REYIS++EERGVD  +LISF+LELSFH + EAY++NTLTD QRN I+DLA+LGLVKLQ
Sbjct:  222 AQLWYIMREYISSAEERGVDPTELISFLLELSFHTLGEAYSLNTLTDVQRNAIRDLAELGLVKLQ 286

[Close]

BLAST of FG532313 vs. TrEMBL
Match: B9FDB6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_16644 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.668e-21
Identity = 49/65 (75.38%), Postives = 59/65 (90.77%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYI+REYIS++EERGVD  +LISF+LELSFH + EAY++NTLTD QRN I+DLA+LGLVKLQ
Sbjct:  179 AQLWYIMREYISSAEERGVDPTELISFLLELSFHTLGEAYSLNTLTDVQRNAIRDLAELGLVKLQ 243

[Close]

BLAST of FG532313 vs. SwissProt
Match: TF2H4_PANTR (General transcription factor IIH subunit 4 OS=Pan troglodytes GN=GTF2H4 PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.296e-7
Identity = 21/62 (33.87%), Postives = 41/62 (66.13%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLV 189
            AQLWY + +Y+  ++ RG+D  +++SF+ +LSF  + + Y++  ++D   N ++ L + GLV
Sbjct:  210 AQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLV 271

[Close]

BLAST of FG532313 vs. SwissProt
Match: TF2H4_MOUSE (General transcription factor IIH subunit 4 OS=Mus musculus GN=Gtf2h4 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.296e-7
Identity = 21/62 (33.87%), Postives = 41/62 (66.13%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLV 189
            AQLWY + +Y+  ++ RG+D  +++SF+ +LSF  + + Y++  ++D   N ++ L + GLV
Sbjct:  211 AQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLV 272

[Close]

BLAST of FG532313 vs. SwissProt
Match: TF2H4_HUMAN (General transcription factor IIH subunit 4 OS=Homo sapiens GN=GTF2H4 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.296e-7
Identity = 21/62 (33.87%), Postives = 41/62 (66.13%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLV 189
            AQLWY + +Y+  ++ RG+D  +++SF+ +LSF  + + Y++  ++D   N ++ L + GLV
Sbjct:  210 AQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLV 271

[Close]

BLAST of FG532313 vs. TAIR peptide
Match: AT4G17020.3 (| Symbols: | transcription factor-related | chr4:9577973-9581082 REVERSE LENGTH=482)

HSP 1 Score: 103.605 bits (257), Expect = 2.549e-23
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI N+EER VD  DLISF+LELSFHV  +AYN+NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYILNAEERDVDPADLISFLLELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TAIR peptide
Match: AT4G17020.1 (| Symbols: | transcription factor-related | chr4:9578152-9581082 REVERSE LENGTH=452)

HSP 1 Score: 103.605 bits (257), Expect = 2.549e-23
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI N+EER VD  DLISF+LELSFHV  +AYN+NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYILNAEERDVDPADLISFLLELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQ 267

[Close]

BLAST of FG532313 vs. TAIR peptide
Match: AT4G17020.2 (| Symbols: | transcription factor-related | chr4:9577939-9581082 REVERSE LENGTH=462)

HSP 1 Score: 103.605 bits (257), Expect = 2.549e-23
Identity = 50/65 (76.92%), Postives = 56/65 (86.15%), Query Frame = 1

Query:    4 AQLWYIIREYISNSEERGVDAGDLISFMLELSFHVIEEAYNINTLTDFQRNIIKDLADLGLVKLQ 198
            AQLWYIIREYI N+EER VD  DLISF+LELSFHV  +AYN+NTLT+ Q N +KDLADLGLVKLQ
Sbjct:  203 AQLWYIIREYILNAEERDVDPADLISFLLELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQ 267

The following BLAST results are available for this feature:

BLAST of FG532313 vs. TrEMBL
BLAST of FG532313 vs. SwissProt
BLAST of FG532313 vs. TAIR peptide

BLAST of FG532313 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5BMX1_VITVI	3.777e-24	86.15	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9RZ76_RICCO	7.124e-23	81.54	Tfiih, polypeptide, putative OS=Ricinus communis G...	[more]
B9H2W7_POPTR	3.535e-22	80.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MC99_ARALY	5.105e-21	78.46	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q7XKD9_ORYSJ	6.668e-21	75.38	OSJNBb0017I01.15 protein OS=Oryza sativa subsp. ja...	[more]
Q680U9_ARATH	6.668e-21	76.92	At4g17020 OS=Arabidopsis thaliana GN=At4g17020 PE=...	[more]
Q680F9_ARATH	6.668e-21	76.92	MRNA, complete cds, clone: RAFL22-56-M10 OS=Arabid...	[more]
Q259V6_ORYSA	6.668e-21	75.38	B0213E10.4 protein OS=Oryza sativa GN=B0213E10.4 P...	[more]
Q0J8Y9_ORYSJ	6.668e-21	75.38	Os04g0680000 protein OS=Oryza sativa subsp. japoni...	[more]
B9FDB6_ORYSJ	6.668e-21	75.38	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG532313 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
TF2H4_PANTR	2.296e-7	33.87	General transcription factor IIH subunit 4 OS=Pan ...	[more]
TF2H4_MOUSE	2.296e-7	33.87	General transcription factor IIH subunit 4 OS=Mus ...	[more]
TF2H4_HUMAN	2.296e-7	33.87	General transcription factor IIH subunit 4 OS=Homo...	[more]

BLAST of FG532313 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G17020.3	2.549e-23	76.92	\| Symbols: \| transcription factor-related \| chr4:...	[more]
AT4G17020.1	2.549e-23	76.92	\| Symbols: \| transcription factor-related \| chr4:...	[more]
AT4G17020.2	2.549e-23	76.92	\| Symbols: \| transcription factor-related \| chr4:...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004598	Transcription factor Tfb2	PANTHER	PTHR13152	TFIIH, POLYPEPTIDE 4	coord: 2..66 score: 1
IPR004598	Transcription factor Tfb2	PFAM	PF03849	Tfb2	coord: 2..65 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG532313 ID=FG532313; Name=FG532313; organism=Pisum sativum; type=EST; length=200bp
AAAGCACAGCTTTGGTACATCATCAGAGAATATATATCTAATTCTGAGGA
AAGAGGTGTTGATGCAGGTGACTTGATCTCATTCATGCTTGAGCTTAGTT
TTCATGTAATTGAGGAGGCATACAATATAAACACATTGACTGATTTCCAG
AGGAACATAATTAAGGACCTAGCAGACCTTGGGCTGGTCAAACTTCAACA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003702	obsolete RNA polymerase II transcription factor activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006281	DNA repair

Vocabulary: **INTERPRO**
Term	Definition
IPR004598	TFIIH_p52/Tfb2

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