FG533309

EST Overview


Name	FG533309
Unique Name	FG533309
Type	EST
Organism	Pisum sativum (pea)
Sequence length	214

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG533309 vs. TrEMBL
Match: B7FLQ0_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.913e-25
Identity = 35/39 (89.74%), Postives = 35/39 (89.74%), Query Frame = 3

Query:   96 LRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            L  PDFEPIAAIGIEIWTMQ GILFDNVLI KDDKIAES
Sbjct:  361 LEKPDFEPIAAIGIEIWTMQDGILFDNVLIAKDDKIAES 399

HSP 2 Score: 66.2402 bits (160), Expect = 4.913e-25
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG WSAP+IDNP YK IWKPQEIPNP YFELE
Sbjct:  330 YKGKWSAPFIDNPAYKGIWKPQEIPNPEYFELE 362

[Close]

BLAST of FG533309 vs. TrEMBL
Match: Q7XAF7_BRANA (Putative papillar cell-specific calnexin OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.735e-22
Identity = 30/36 (83.33%), Postives = 34/36 (94.44%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD+EPIAAIGIEIWTMQ GILFDN+LI KD+K+AES
Sbjct:  360 PDYEPIAAIGIEIWTMQDGILFDNILIAKDEKVAES 395

HSP 2 Score: 60.8474 bits (146), Expect = 2.735e-22
Identity = 25/33 (75.76%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG WSAP IDNP YK IWKP++IPNP YFEL+
Sbjct:  326 YKGKWSAPMIDNPAYKGIWKPRDIPNPDYFELD 358

[Close]

BLAST of FG533309 vs. TrEMBL
Match: B9ICC7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_733819 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.547e-22
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQEIPNP YFEL+
Sbjct:  328 YKGKWHAPLIDNPNYKGIWKPQEIPNPDYFELD 360

HSP 2 Score: 63.929 bits (154), Expect = 3.547e-22
Identity = 27/36 (75.00%), Postives = 33/36 (91.67%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            P+FEPIAA+GIEIWTMQ GILFDN+LI +D+K+A S
Sbjct:  362 PEFEPIAAVGIEIWTMQDGILFDNILIAQDEKVASS 397

[Close]

BLAST of FG533309 vs. TrEMBL
Match: B9RA41_RICCO (Calnexin, putative OS=Ricinus communis GN=RCOM_1503060 PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 4.613e-22
Identity = 28/33 (84.85%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQEIPNP YFELE
Sbjct:  330 YKGKWHAPLIDNPNYKGIWKPQEIPNPNYFELE 362

HSP 2 Score: 62.3882 bits (150), Expect = 4.613e-22
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 3

Query:   96 LRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            L  PDFEPIAA+GIEIWTMQ GILF ++LI  D+K+AES
Sbjct:  361 LEKPDFEPIAAVGIEIWTMQDGILFGHILIADDEKVAES 399

[Close]

BLAST of FG533309 vs. TrEMBL
Match: B6TNF1_MAIZE (Calnexin OS=Zea mays PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.614e-22
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQEIPNP YFEL+
Sbjct:  321 YKGKWHAPMIDNPNYKGIWKPQEIPNPEYFELD 353

HSP 2 Score: 63.5438 bits (153), Expect = 4.614e-22
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PDF+PIAAIGIEIWTMQ GILFDN+LI  D+K+A S
Sbjct:  355 PDFDPIAAIGIEIWTMQDGILFDNILIADDEKVATS 390

[Close]

BLAST of FG533309 vs. TrEMBL
Match: B4FAK8_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.615e-22
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQEIPNP YFEL+
Sbjct:  321 YKGKWHAPMIDNPNYKGIWKPQEIPNPEYFELD 353

HSP 2 Score: 63.5438 bits (153), Expect = 4.615e-22
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PDF+PIAAIGIEIWTMQ GILFDN+LI  D+K+A S
Sbjct:  355 PDFDPIAAIGIEIWTMQDGILFDNILIADDEKVATS 390

[Close]

BLAST of FG533309 vs. TrEMBL
Match: Q41798_MAIZE (Calnexin (Fragment) OS=Zea mays GN=CNX PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.630e-22
Identity = 27/33 (81.82%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQEIPNP YFEL+
Sbjct:  215 YKGKWHAPMIDNPNYKGIWKPQEIPNPEYFELD 247

HSP 2 Score: 63.5438 bits (153), Expect = 4.630e-22
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PDF+PIAAIGIEIWTMQ GILFDN+LI  D+K+A S
Sbjct:  249 PDFDPIAAIGIEIWTMQDGILFDNILIADDEKVATS 284

[Close]

BLAST of FG533309 vs. TrEMBL
Match: A9PHA6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_823453 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.630e-22
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PDFEPIAA+GIEIWTMQ GILFDN+L+ KD+K A S
Sbjct:  361 PDFEPIAAVGIEIWTMQDGILFDNILVAKDEKAAAS 396

HSP 2 Score: 63.5438 bits (153), Expect = 4.630e-22
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG W AP IDNPNYK IWKPQ+IPNP YFEL+
Sbjct:  327 YKGKWHAPLIDNPNYKGIWKPQDIPNPDYFELD 359

[Close]

BLAST of FG533309 vs. TrEMBL
Match: Q3LVM3_TAROF (TO49-2 (Fragment) OS=Taraxacum officinale GN=To49-2 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 6.164e-22
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 3

Query:   96 LRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            L  P+FEPIAAIGIEIWTMQ GILFDN+LI  D+K AES
Sbjct:   90 LESPNFEPIAAIGIEIWTMQDGILFDNILIASDEKTAES 128

HSP 2 Score: 63.1586 bits (152), Expect = 6.164e-22
Identity = 27/34 (79.41%), Postives = 28/34 (82.35%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELEN 103
            YKG W AP IDNP YK IWKPQEIPNP YFELE+
Sbjct:   59 YKGKWHAPLIDNPAYKGIWKPQEIPNPGYFELES 92

[Close]

BLAST of FG533309 vs. TrEMBL
Match: A5BCC8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041895 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.016e-21
Identity = 26/34 (76.47%), Postives = 29/34 (85.29%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELEN 103
            YKG W AP IDNPNYK IWKPQ+IPNP YFEL++
Sbjct:  328 YKGKWHAPLIDNPNYKGIWKPQQIPNPDYFELDH 361

HSP 2 Score: 63.1586 bits (152), Expect = 1.016e-21
Identity = 28/36 (77.78%), Postives = 31/36 (86.11%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD EP+AAIGIEIWTMQ GILFDN+LI  D+K AES
Sbjct:  362 PDIEPVAAIGIEIWTMQDGILFDNILITSDEKTAES 397

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_PEA (Calnexin homolog OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 9.903e-28
Identity = 35/39 (89.74%), Postives = 35/39 (89.74%), Query Frame = 3

Query:   96 LRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            L  PDFEPIAAIGIEIWTMQ GILFDNVLI KDDKIAES
Sbjct:  365 LEKPDFEPIAAIGIEIWTMQDGILFDNVLIAKDDKIAES 403

HSP 2 Score: 70.8626 bits (172), Expect = 9.903e-28
Identity = 30/33 (90.91%), Postives = 30/33 (90.91%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG WSAPYIDNPNYK IWKPQEIPNP YFELE
Sbjct:  334 YKGKWSAPYIDNPNYKGIWKPQEIPNPEYFELE 366

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX1_ARATH (Calnexin homolog 1 OS=Arabidopsis thaliana GN=CNX1 PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.331e-23
Identity = 29/36 (80.56%), Postives = 34/36 (94.44%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD+EPIAAIGIEIWTMQ GILFDN+LI KD+K+AE+
Sbjct:  360 PDYEPIAAIGIEIWTMQDGILFDNILIAKDEKVAET 395

HSP 2 Score: 59.6918 bits (143), Expect = 6.331e-23
Identity = 24/33 (72.73%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG WS+P IDNP YK IWKP++IPNP YFEL+
Sbjct:  326 YKGKWSSPLIDNPAYKGIWKPRDIPNPDYFELD 358

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_ASPFU (Calnexin homolog OS=Aspergillus fumigatus GN=AFUA_4G12850 PE=2 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 4.650e-15
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W+AP IDNP YK IWKP++IPNP YFE
Sbjct:  356 YKGKWTAPLIDNPAYKGIWKPRKIPNPAYFE 386

HSP 2 Score: 43.1282 bits (100), Expect = 4.650e-15
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 3

Query:  108 DFEPIAAIGIEIWTMQHGILFDNVLI 185
            +FEP+ A+G EIWTMQ+ ILFDN+ I
Sbjct:  392 NFEPMGAVGFEIWTMQNDILFDNIYI 417

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_SOYBN (Calnexin homolog OS=Glycine max PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.213e-12
Identity = 36/63 (57.14%), Postives = 39/63 (61.90%), Query Frame = 3

Query:   24 PTLITPIIRLFGSLRRFQTHSTLNLRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            P +  P  +     R         L  PDFEPIAAIGIEIWTMQ GILFDNVLI  DDK+AES
Sbjct:  342 PYIDNPSYKGIWKPREIPNPEYFELAKPDFEPIAAIGIEIWTMQDGILFDNVLIANDDKVAES 404

HSP 2 Score: 65.4698 bits (158), Expect = 9.800e-11
Identity = 27/32 (84.38%), Postives = 29/32 (90.62%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFEL 97
            YKG WSAPYIDNP+YK IWKP+EIPNP YFEL
Sbjct:  335 YKGKWSAPYIDNPSYKGIWKPREIPNPEYFEL 366

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_CANFA (Calnexin OS=Canis familiaris GN=CANX PE=1 SV=3)

HSP 1 Score: 54.6842 bits (130), Expect = 5.394e-12
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P IDNPNY+ IWKP++IPNP +FE
Sbjct:  380 YKGKWKPPMIDNPNYQGIWKPRKIPNPDFFE 410

HSP 2 Score: 35.039 bits (79), Expect = 5.394e-12
Identity = 12/31 (38.71%), Postives = 21/31 (67.74%), Query Frame = 3

Query:  117 PIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            P +AIG+E+W+M   I FDN ++  D ++ +
Sbjct:  419 PFSAIGLELWSMTSDIFFDNFIVCGDRRVVD 449

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_RAT (Calnexin OS=Rattus norvegicus GN=Canx PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.395e-12
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P IDNPNY+ IWKP++IPNP +FE
Sbjct:  380 YKGKWKPPMIDNPNYQGIWKPRKIPNPDFFE 410

HSP 2 Score: 35.039 bits (79), Expect = 5.395e-12
Identity = 13/31 (41.94%), Postives = 21/31 (67.74%), Query Frame = 3

Query:  117 PIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            P +AIG+E+W+M   I FDN +I  D ++ +
Sbjct:  419 PFSAIGLELWSMTSDIFFDNFIISGDRRVVD 449

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_MOUSE (Calnexin OS=Mus musculus GN=Canx PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 5.395e-12
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P IDNPNY+ IWKP++IPNP +FE
Sbjct:  380 YKGKWKPPMIDNPNYQGIWKPRKIPNPDFFE 410

HSP 2 Score: 35.039 bits (79), Expect = 5.395e-12
Identity = 13/31 (41.94%), Postives = 21/31 (67.74%), Query Frame = 3

Query:  117 PIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            P +AIG+E+W+M   I FDN +I  D ++ +
Sbjct:  419 PFSAIGLELWSMTSDIFFDNFIISGDRRVVD 449

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_PONAB (Calnexin OS=Pongo abelii GN=CANX PE=2 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 9.090e-12
Identity = 20/31 (64.52%), Postives = 25/31 (80.65%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P IDNP+Y+ IWKP++IPNP +FE
Sbjct:  379 YKGKWKPPMIDNPSYQGIWKPRKIPNPDFFE 409

HSP 2 Score: 36.1946 bits (82), Expect = 9.090e-12
Identity = 14/31 (45.16%), Postives = 21/31 (67.74%), Query Frame = 3

Query:  117 PIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            P +AIG+E+W+M   I FDN +I  D +I +
Sbjct:  418 PFSAIGLELWSMTSDIFFDNFIICADRRIVD 448

[Close]

BLAST of FG533309 vs. SwissProt
Match: CALX_HUMAN (Calnexin OS=Homo sapiens GN=CANX PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 9.090e-12
Identity = 20/31 (64.52%), Postives = 25/31 (80.65%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P IDNP+Y+ IWKP++IPNP +FE
Sbjct:  379 YKGKWKPPMIDNPSYQGIWKPRKIPNPDFFE 409

HSP 2 Score: 36.1946 bits (82), Expect = 9.090e-12
Identity = 14/31 (45.16%), Postives = 21/31 (67.74%), Query Frame = 3

Query:  117 PIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            P +AIG+E+W+M   I FDN +I  D +I +
Sbjct:  418 PFSAIGLELWSMTSDIFFDNFIICADRRIVD 448

[Close]

BLAST of FG533309 vs. SwissProt
Match: CLGN_HUMAN (Calmegin OS=Homo sapiens GN=CLGN PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 7.311e-11
Identity = 20/31 (64.52%), Postives = 24/31 (77.42%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFE 94
            YKG W  P +DNPNY+ IW P++IPNP YFE
Sbjct:  368 YKGVWRPPLVDNPNYQGIWSPRKIPNPDYFE 398

HSP 2 Score: 32.3426 bits (72), Expect = 7.311e-11
Identity = 11/29 (37.93%), Postives = 21/29 (72.41%), Query Frame = 3

Query:  123 AAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            +A+G+E+W+M   I FDN +I  + ++A+
Sbjct:  409 SALGLELWSMTSDIYFDNFIICSEKEVAD 437

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT5G61790.1 (| Symbols: CNX1, ATCNX1 | calnexin 1 | chr5:24827394-24829642 REVERSE LENGTH=530)

HSP 1 Score: 67.0106 bits (162), Expect = 5.096e-24
Identity = 29/36 (80.56%), Postives = 34/36 (94.44%), Query Frame = 3

Query:  105 PDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD+EPIAAIGIEIWTMQ GILFDN+LI KD+K+AE+
Sbjct:  360 PDYEPIAAIGIEIWTMQDGILFDNILIAKDEKVAET 395

HSP 2 Score: 59.6918 bits (143), Expect = 5.096e-24
Identity = 24/33 (72.73%), Postives = 28/33 (84.85%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELE 100
            YKG WS+P IDNP YK IWKP++IPNP YFEL+
Sbjct:  326 YKGKWSSPLIDNPAYKGIWKPRDIPNPDYFELD 358

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT5G07340.2 (| Symbols: | Calreticulin family protein | chr5:2317300-2319458 FORWARD LENGTH=540)

HSP 1 Score: 63.929 bits (154), Expect = 2.303e-11
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 3

Query:   24 PTLITPIIRLFGSLRRFQTHSTLNLRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            P +  P  +     R         L  P+ EPIAAIGIEIWTMQ GILFDN+LI KD+K+AE+
Sbjct:  343 PLIDNPAYKGIWKPRDIPNPDYFELERPNLEPIAAIGIEIWTMQDGILFDNILISKDEKVAET 405

HSP 2 Score: 62.003 bits (149), Expect = 8.726e-11
Identity = 26/37 (70.27%), Postives = 29/37 (78.38%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELENTRL 112
            YKG WS+P IDNP YK IWKP++IPNP YFELE   L
Sbjct:  336 YKGKWSSPLIDNPAYKGIWKPRDIPNPDYFELERPNL 372

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT5G07340.1 (| Symbols: | Calreticulin family protein | chr5:2317300-2319458 FORWARD LENGTH=532)

HSP 1 Score: 63.929 bits (154), Expect = 2.303e-11
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 3

Query:   24 PTLITPIIRLFGSLRRFQTHSTLNLRIPDFEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAES 212
            P +  P  +     R         L  P+ EPIAAIGIEIWTMQ GILFDN+LI KD+K+AE+
Sbjct:  335 PLIDNPAYKGIWKPRDIPNPDYFELERPNLEPIAAIGIEIWTMQDGILFDNILISKDEKVAET 397

HSP 2 Score: 62.003 bits (149), Expect = 8.726e-11
Identity = 26/37 (70.27%), Postives = 29/37 (78.38%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP*YFELENTRL 112
            YKG WS+P IDNP YK IWKP++IPNP YFELE   L
Sbjct:  328 YKGKWSSPLIDNPAYKGIWKPRDIPNPDYFELERPNL 364

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT1G56340.1 (| Symbols: CRT1, CRT1a, AtCRT1a | calreticulin 1a | chr1:21090059-21092630 REVERSE LENGTH=425)

HSP 1 Score: 38.1206 bits (87), Expect = 5.278e-8
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 2

Query:    8 G*WSAPYIDNPNYKAIWKPQEIPNP*Y 88
            G W+AP I NP Y   WKP++I NP Y
Sbjct:  263 GEWTAPTIPNPEYNGEWKPKKIKNPAY 289

HSP 2 Score: 34.2686 bits (77), Expect = 5.278e-8
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  111 FEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            F  +  +G+E+W ++ G LFDNVL+  D + A+
Sbjct:  312 FPKLKYVGVELWQVKSGSLFDNVLVSDDPEYAK 344

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT1G56340.2 (| Symbols: CRT1, CRT1a, AtCRT1a | calreticulin 1a | chr1:21090022-21092630 REVERSE LENGTH=424)

HSP 1 Score: 38.1206 bits (87), Expect = 5.279e-8
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 2

Query:    8 G*WSAPYIDNPNYKAIWKPQEIPNP*Y 88
            G W+AP I NP Y   WKP++I NP Y
Sbjct:  263 GEWTAPTIPNPEYNGEWKPKKIKNPAY 289

HSP 2 Score: 34.2686 bits (77), Expect = 5.279e-8
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  111 FEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            F  +  +G+E+W ++ G LFDNVL+  D + A+
Sbjct:  312 FPKLKYVGVELWQVKSGSLFDNVLVSDDPEYAK 344

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT1G09210.1 (| Symbols: CRT1b, AtCRT1b | calreticulin 1b | chr1:2973217-2976655 REVERSE LENGTH=424)

HSP 1 Score: 38.1206 bits (87), Expect = 6.837e-8
Identity = 15/27 (55.56%), Postives = 18/27 (66.67%), Query Frame = 2

Query:    8 G*WSAPYIDNPNYKAIWKPQEIPNP*Y 88
            G W+AP I NP Y   WKP++I NP Y
Sbjct:  263 GEWTAPTIPNPEYMGEWKPKQIKNPNY 289

HSP 2 Score: 33.8834 bits (76), Expect = 6.837e-8
Identity = 14/33 (42.42%), Postives = 21/33 (63.64%), Query Frame = 3

Query:  111 FEPIAAIGIEIWTMQHGILFDNVLIVKDDKIAE 209
            F  +  +G+E+W ++ G LFDNVLI  D   A+
Sbjct:  312 FPKLKYVGLELWQVKSGSLFDNVLICDDPDYAK 344

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT1G08450.1 (| Symbols: CRT3, PSL1, EBS2, AtCRT3 | calreticulin 3 | chr1:2668008-2671800 REVERSE LENGTH=424)

HSP 1 Score: 36.5798 bits (83), Expect = 4.174e-7
Identity = 16/27 (59.26%), Postives = 16/27 (59.26%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP 82
            YKG W A  I NPNYK  WK   I NP
Sbjct:  281 YKGPWKAKRIKNPNYKGKWKNPWIDNP 307

HSP 2 Score: 32.7278 bits (73), Expect = 4.174e-7
Identity = 16/39 (41.03%), Postives = 23/39 (58.97%), Query Frame = 3

Query:  105 PDFEPIAAI---GIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD   + +I   GIE+W ++ G +FDN+LI  D   A S
Sbjct:  313 PDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARS 351

[Close]

BLAST of FG533309 vs. TAIR peptide
Match: AT1G08450.2 (| Symbols: CRT3, PSL1, EBS2, AtCRT3 | calreticulin 3 | chr1:2668008-2671800 REVERSE LENGTH=370)

HSP 1 Score: 36.5798 bits (83), Expect = 4.195e-7
Identity = 16/27 (59.26%), Postives = 16/27 (59.26%), Query Frame = 2

Query:    2 YKG*WSAPYIDNPNYKAIWKPQEIPNP 82
            YKG W A  I NPNYK  WK   I NP
Sbjct:  227 YKGPWKAKRIKNPNYKGKWKNPWIDNP 253

HSP 2 Score: 32.7278 bits (73), Expect = 4.195e-7
Identity = 16/39 (41.03%), Postives = 23/39 (58.97%), Query Frame = 3

Query:  105 PDFEPIAAI---GIEIWTMQHGILFDNVLIVKDDKIAES 212
            PD   + +I   GIE+W ++ G +FDN+LI  D   A S
Sbjct:  259 PDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARS 297

The following BLAST results are available for this feature:

BLAST of FG533309 vs. TrEMBL
BLAST of FG533309 vs. SwissProt
BLAST of FG533309 vs. TAIR peptide

BLAST of FG533309 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FLQ0_MEDTR	4.913e-25	89.74	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q7XAF7_BRANA	2.735e-22	83.33	Putative papillar cell-specific calnexin OS=Brassi...	[more]
B9ICC7_POPTR	3.547e-22	81.82	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RA41_RICCO	4.613e-22	84.85	Calnexin, putative OS=Ricinus communis GN=RCOM_150...	[more]
B6TNF1_MAIZE	4.614e-22	81.82	Calnexin OS=Zea mays PE=2 SV=1	[more]
B4FAK8_MAIZE	4.615e-22	81.82	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q41798_MAIZE	4.630e-22	81.82	Calnexin (Fragment) OS=Zea mays GN=CNX PE=2 SV=1	[more]
A9PHA6_POPTR	4.630e-22	77.78	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q3LVM3_TAROF	6.164e-22	76.92	TO49-2 (Fragment) OS=Taraxacum officinale GN=To49-...	[more]
A5BCC8_VITVI	1.016e-21	76.47	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of FG533309 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CALX_PEA	9.903e-28	89.74	Calnexin homolog OS=Pisum sativum PE=2 SV=1	[more]
CALX1_ARATH	6.331e-23	80.56	Calnexin homolog 1 OS=Arabidopsis thaliana GN=CNX1...	[more]
CALX_ASPFU	4.650e-15	74.19	Calnexin homolog OS=Aspergillus fumigatus GN=AFUA_...	[more]
CALX_SOYBN	5.213e-12	57.14	Calnexin homolog OS=Glycine max PE=2 SV=1	[more]
CALX_CANFA	5.394e-12	67.74	Calnexin OS=Canis familiaris GN=CANX PE=1 SV=3	[more]
CALX_RAT	5.395e-12	67.74	Calnexin OS=Rattus norvegicus GN=Canx PE=1 SV=1	[more]
CALX_MOUSE	5.395e-12	67.74	Calnexin OS=Mus musculus GN=Canx PE=1 SV=1	[more]
CALX_PONAB	9.090e-12	64.52	Calnexin OS=Pongo abelii GN=CANX PE=2 SV=2	[more]
CALX_HUMAN	9.090e-12	64.52	Calnexin OS=Homo sapiens GN=CANX PE=1 SV=2	[more]
CLGN_HUMAN	7.311e-11	64.52	Calmegin OS=Homo sapiens GN=CLGN PE=1 SV=1	[more]

BLAST of FG533309 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 8


Match Name	E-value	Identity	Description
AT5G61790.1	5.096e-24	80.56	\| Symbols: CNX1, ATCNX1 \| calnexin 1 \| chr5:248273...	[more]
AT5G07340.2	2.303e-11	50.79	\| Symbols: \| Calreticulin family protein \| chr5:2...	[more]
AT5G07340.1	2.303e-11	50.79	\| Symbols: \| Calreticulin family protein \| chr5:2...	[more]
AT1G56340.1	5.278e-8	55.56	\| Symbols: CRT1, CRT1a, AtCRT1a \| calreticulin 1a ...	[more]
AT1G56340.2	5.279e-8	55.56	\| Symbols: CRT1, CRT1a, AtCRT1a \| calreticulin 1a ...	[more]
AT1G09210.1	6.837e-8	55.56	\| Symbols: CRT1b, AtCRT1b \| calreticulin 1b \| chr1...	[more]
AT1G08450.1	4.174e-7	59.26	\| Symbols: CRT3, PSL1, EBS2, AtCRT3 \| calreticulin...	[more]
AT1G08450.2	4.195e-7	59.26	\| Symbols: CRT3, PSL1, EBS2, AtCRT3 \| calreticulin...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001580	Calreticulin/calnexin	PANTHER	PTHR11073	CALRETICULIN AND CALNEXIN	coord: 37..70 score: 7.4
IPR001580	Calreticulin/calnexin	PFAM	PF00262	Calreticulin	coord: 36..61 score: 2
None	No IPR available	PANTHER	PTHR11073:SF1	CALNEXIN	coord: 37..70 score: 7.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG533309 ID=FG533309; Name=FG533309; organism=Pisum sativum; type=EST; length=214bp
TTATAAGGGATAATGGAGTGCCCCCTACATTGATAACCCCAATTATAAGG
CTATTTGGAAGCCTCAGGAGATTCCAAACCCATAGTACTTTGAACTTGAG
AATACCAGACTTTGAGCCTATTGCTGCTATTGGCATTGAAATTTGGACAA
TGCAACATGGCATCCTATTTGATAATGTTCTCATAGTTAAAGATGATAAG
ATTGCCGAGTCCTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001580	Calret/calnex

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