CD859470

EST Overview


Name	CD859470
Unique Name	CD859470
Type	EST
Organism	Pisum sativum (pea)
Sequence length	476

Libraries


Library Name	Type
LIBEST_014000 F15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859470 vs. TrEMBL
Match: B7FGZ8_MEDTR (Proteasome subunit beta type OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.255e-60
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 1

Query:  127 MDQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            MD+VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW
Sbjct:    1 MDKVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 116

[Close]

BLAST of CD859470 vs. TrEMBL
Match: C6SVE5_SOYBN (Proteasome subunit beta type OS=Glycine max PE=2 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 8.868e-58
Identity = 114/117 (97.44%), Postives = 115/117 (98.29%), Query Frame = 1

Query:  127 MDQ-VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            MDQ VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQ+VSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKN LQTGLIVGGW
Sbjct:    1 MDQKVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNFLQTGLIVGGW 117

[Close]

BLAST of CD859470 vs. TrEMBL
Match: B4UWD9_ARAHY (Threonine endopeptidase (Fragment) OS=Arachis hypogaea PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.184e-56
Identity = 109/113 (96.46%), Postives = 112/113 (99.12%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            +DFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLL+YNNKN L+TGLIVGGW
Sbjct:    1 MDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGW 113

[Close]

BLAST of CD859470 vs. TrEMBL
Match: B9SPS6_RICCO (Proteasome subunit beta type OS=Ricinus communis GN=RCOM_0205360 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.045e-54
Identity = 104/113 (92.04%), Postives = 110/113 (97.35%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            +D  APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQ+VSDYVRYFLHQHTIQLGQPATVKVAANLVRL++YNNKN L+TGLIVGGW
Sbjct:    1 MDLKAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLVRLISYNNKNMLETGLIVGGW 113

[Close]

BLAST of CD859470 vs. TrEMBL
Match: A9PD83_POPTR (Proteasome subunit beta type OS=Populus trichocarpa GN=POPTRDRAFT_673509 PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.015e-53
Identity = 103/112 (91.96%), Postives = 108/112 (96.43%), Query Frame = 1

Query:  139 DFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            + N PHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVY+CRSGSAADSQ VSDYVRYFLHQHTIQLGQPATVKVAANLVR+L+YNNKN LQTG+IVGGW
Sbjct:    8 EINGPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYLCRSGSAADSQTVSDYVRYFLHQHTIQLGQPATVKVAANLVRMLSYNNKNFLQTGMIVGGW 119

[Close]

BLAST of CD859470 vs. TrEMBL
Match: A9NLJ2_PICSI (Proteasome subunit beta type OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.015e-53
Identity = 104/116 (89.66%), Postives = 109/116 (93.97%), Query Frame = 1

Query:  127 MDQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            MD    NAPHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVY+CRSGSAADSQVVSDYVRYFLHQH IQLGQPATVKV ANL+RLL+YNNKN L+TGLIVGGW
Sbjct:    1 MDMDWMNAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYLCRSGSAADSQVVSDYVRYFLHQHIIQLGQPATVKVTANLIRLLSYNNKNTLETGLIVGGW 116

[Close]

BLAST of CD859470 vs. TrEMBL
Match: B9HBA5_POPTR (Proteasome subunit beta type OS=Populus trichocarpa GN=POPTRDRAFT_819127 PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.731e-53
Identity = 101/115 (87.83%), Postives = 110/115 (95.65%), Query Frame = 1

Query:  130 DQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            ++ + N PHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVY+CRSGSAADSQ VSDYVRYFLHQHTIQLGQPATVKVAANLVRLL+Y+NKN LQTG+I+GGW
Sbjct:    5 EESEINGPHSMGTTIIGVTYNGGVVLGADSRTSTGIYVANRASDKITQLTDNVYLCRSGSAADSQTVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYSNKNMLQTGMIIGGW 119

[Close]

BLAST of CD859470 vs. TrEMBL
Match: A9P9E4_POPTR (Proteasome subunit beta type OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.731e-53
Identity = 101/115 (87.83%), Postives = 110/115 (95.65%), Query Frame = 1

Query:  130 DQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            ++ + N PHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVY+CRSGSAADSQ VSDYVRYFLHQHTIQLGQPATVKVAANLVRLL+Y+NKN LQTG+I+GGW
Sbjct:    5 EESEINGPHSMGTTIIGVTYNGGVVLGADSRTSTGIYVANRASDKITQLTDNVYLCRSGSAADSQTVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYSNKNMLQTGMIIGGW 119

[Close]

BLAST of CD859470 vs. TrEMBL
Match: D7T0M7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_65.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032253001 PE=4 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.260e-53
Identity = 102/110 (92.73%), Postives = 108/110 (98.18%), Query Frame = 1

Query:  145 NAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            N PHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVY+CRSGSAADSQ++SDYVRY+LHQHTIQLGQPATVKVAANLVRL++YNNKN LQTGLIVGGW
Sbjct:    5 NQPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYLCRSGSAADSQILSDYVRYYLHQHTIQLGQPATVKVAANLVRLISYNNKNMLQTGLIVGGW 114

[Close]

BLAST of CD859470 vs. TrEMBL
Match: A9NMI9_PICSI (Proteasome subunit beta type OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 2.952e-53
Identity = 103/116 (88.79%), Postives = 108/116 (93.10%), Query Frame = 1

Query:  127 MDQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            MD    NAPHSMGTTIIGVTYNGG VLGADSRTSTG+YVANRASDKITQLTDNVY+CRSGSAADSQVVSDYVRYFLHQH IQLGQPATVKV ANL+RLL+YNNKN L+TGLIVGGW
Sbjct:    1 MDMDWINAPHSMGTTIIGVTYNGGTVLGADSRTSTGMYVANRASDKITQLTDNVYLCRSGSAADSQVVSDYVRYFLHQHIIQLGQPATVKVTANLIRLLSYNNKNTLETGLIVGGW 116

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_TOBAC (Proteasome subunit beta type-6 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 4.206e-55
Identity = 102/116 (87.93%), Postives = 110/116 (94.83%), Query Frame = 1

Query:  127 MDQVDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            M+  D + PHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQ+VSDYVRYFLHQHTIQLGQPATVKVAANL RLL+YNNK+ LQTG+I+GGW
Sbjct:    1 MENTDVDQPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLTRLLSYNNKDRLQTGMIIGGW 116

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_ARATH (Proteasome subunit beta type-6 OS=Arabidopsis thaliana GN=PBA1 PE=1 SV=2)

HSP 1 Score: 211.846 bits (538), Expect = 1.224e-54
Identity = 103/113 (91.15%), Postives = 110/113 (97.35%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            ++ +APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQ GQPATVKV+ANL+R+LAYNNKN LQTGLIVGGW
Sbjct:    3 LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGW 115

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_DICDI (Proteasome subunit beta type-6 OS=Dictyostelium discoideum GN=psmB6 PE=1 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 3.830e-32
Identity = 61/105 (58.10%), Postives = 82/105 (78.10%), Query Frame = 1

Query:  160 MGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            +GT+I+ V Y+GGV++GADSRT+TG Y+ANR ++KIT + + +Y CRSGSAAD+Q +SDYVRY+L  HT +L     VK AA+L +LL Y+NKNNL  G+IV GW
Sbjct:   13 LGTSIMAVEYDGGVIMGADSRTTTGAYIANRVTNKITPIHERIYCCRSGSAADTQAISDYVRYYLEMHTSELCDEPDVKTAASLFQLLCYSNKNNLMAGIIVAGW 117

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_SCHPO (Probable proteasome subunit beta type-6 OS=Schizosaccharomyces pombe GN=SPBC4C3.10c PE=2 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 9.763e-28
Identity = 61/117 (52.14%), Postives = 78/117 (66.67%), Query Frame = 1

Query:  136 VDFNAPHS----MGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            VD NA       MGTTI  + Y  GV+L ADSRT+ G Y+ANR +DK+TQLTDN++ CRSGSAAD+Q V+D ++Y+L  + IQ G   +V  AA L   + Y NKN L  GLIV G+
Sbjct:   11 VDINAIKKGEIRMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGY 127

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_MOUSE (Proteasome subunit beta type-6 OS=Mus musculus GN=Psmb6 PE=1 SV=3)

HSP 1 Score: 122.094 bits (305), Expect = 1.275e-27
Identity = 57/110 (51.82%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  145 NAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            N   S GTTI+ V +NGGVVLGADSRT+TG Y+ANR +DK+T + D+++ CRSGSAAD+Q V+D V Y L  H+I+L +P  V  AA+L + + Y  + +L  G+I+ GW
Sbjct:   27 NREVSTGTTIMAVQFNGGVVLGADSRTTTGSYIANRVTDKLTPIHDHIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGW 136

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_RAT (Proteasome subunit beta type-6 OS=Rattus norvegicus GN=Psmb6 PE=1 SV=3)

HSP 1 Score: 120.168 bits (300), Expect = 4.845e-27
Identity = 56/110 (50.91%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  145 NAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            N   S GTTI+ V ++GGVVLGADSRT+TG Y+ANR +DK+T + D+++ CRSGSAAD+Q V+D V Y L  H+I+L +P  V  AA+L + + Y  + +L  G+I+ GW
Sbjct:   27 NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDHIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGW 136

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_YEAST (Proteasome component PRE3 OS=Saccharomyces cerevisiae GN=PRE3 PE=1 SV=2)

HSP 1 Score: 119.783 bits (299), Expect = 6.328e-27
Identity = 59/118 (50.00%), Postives = 85/118 (72.03%), Query Frame = 1

Query:  133 QVDFN----APHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            QVD N       S+GT+I+ VT+  GV+LGADSRT+TG Y+ANR +DK+T++ D ++ CRSGSAAD+Q ++D V+Y L  +T Q G P+T + AA++ + L Y NK+NL  G+IV G+
Sbjct:    5 QVDINRLKKGEVSLGTSIMAVTFKDGVILGADSRTTTGAYIANRVTDKLTRVHDKIWCCRSGSAADTQAIADIVQYHLELYTSQYGTPST-ETAASVFKELCYENKDNLTAGIIVAGY 121

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_BOVIN (Proteasome subunit beta type-6 OS=Bos taurus GN=PSMB6 PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 6.328e-27
Identity = 56/110 (50.91%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  145 NAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            N   S GTTI+ V ++GGVVLGADSRT+TG Y+ANR +DK+T + D ++ CRSGSAAD+Q V+D V Y L  H+I+L +P  V  AA+L + + Y  + +L  G+I+ GW
Sbjct:   28 NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGW 137

[Close]

BLAST of CD859470 vs. SwissProt
Match: PSB6_HUMAN (Proteasome subunit beta type-6 OS=Homo sapiens GN=PSMB6 PE=1 SV=4)

HSP 1 Score: 119.398 bits (298), Expect = 8.265e-27
Identity = 55/106 (51.89%), Postives = 77/106 (72.64%), Query Frame = 1

Query:  157 SMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            S GTTI+ V ++GGVVLGADSRT+TG Y+ANR +DK+T + D ++ CRSGSAAD+Q V+D V Y L  H+I+L +P  V  AA+L + + Y  + +L  G+I+ GW
Sbjct:   32 STGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGW 137

[Close]

BLAST of CD859470 vs. SwissProt
Match: PB6LB_SALSA (Proteasome subunit beta type-6-B like protein OS=Salmo salar GN=psmb6l-b PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 6.129e-22
Identity = 48/107 (44.86%), Postives = 73/107 (68.22%), Query Frame = 1

Query:  157 SMGTTIIGVTYNGGVVLGADSRTSTG-VYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            S GTTI+ VT+NGGV++G+DSR S G  YV+++  +K+ Q+ D ++ C +GS AD+Q V+   ++ +  H+IQ+  P  VK AA++++ L YNNK  LQ G I  GW
Sbjct:   14 STGTTILAVTFNGGVIIGSDSRASIGGSYVSSKTINKLIQVHDRIFCCIAGSLADAQAVTKAAKFQISFHSIQMESPPLVKAAASVLKELCYNNKEELQAGFITAGW 120

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT4G31300.3 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=233)

HSP 1 Score: 211.846 bits (538), Expect = 1.392e-55
Identity = 103/113 (91.15%), Postives = 110/113 (97.35%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            ++ +APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQ GQPATVKV+ANL+R+LAYNNKN LQTGLIVGGW
Sbjct:    3 LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGW 115

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT4G31300.1 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=233)

HSP 1 Score: 211.846 bits (538), Expect = 1.392e-55
Identity = 103/113 (91.15%), Postives = 110/113 (97.35%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGGW 474
            ++ +APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQ GQPATVKV+ANL+R+LAYNNKN LQTGLIVGGW
Sbjct:    3 LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGW 115

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT4G31300.2 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=234)

HSP 1 Score: 207.994 bits (528), Expect = 2.010e-54
Identity = 103/114 (90.35%), Postives = 110/114 (96.49%), Query Frame = 1

Query:  136 VDFNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNN-LQTGLIVGGW 474
            ++ +APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQ GQPATVKV+ANL+R+LAYNNK N LQTGLIVGGW
Sbjct:    3 LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKQNMLQTGLIVGGW 116

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT5G40580.2 (| Symbols: PBB2 | 20S proteasome beta subunit PBB2 | chr5:16248511-16250451 REVERSE LENGTH=274)

HSP 1 Score: 81.2629 bits (199), Expect = 2.838e-16
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGG 141

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT5G40580.1 (| Symbols: PBB2 | 20S proteasome beta subunit PBB2 | chr5:16248511-16250451 REVERSE LENGTH=274)

HSP 1 Score: 81.2629 bits (199), Expect = 2.838e-16
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGG 141

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT3G27430.2 (| Symbols: PBB1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr3:10152910-10155052 FORWARD LENGTH=273)

HSP 1 Score: 80.8777 bits (198), Expect = 3.706e-16
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGG 141

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT3G27430.1 (| Symbols: PBB1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr3:10152910-10155036 FORWARD LENGTH=267)

HSP 1 Score: 80.8777 bits (198), Expect = 3.706e-16
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI+G+ +  GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGG 141

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT5G40580.3 (| Symbols: PBB2 | 20S proteasome beta subunit PBB2 | chr5:16248511-16250451 REVERSE LENGTH=268)

HSP 1 Score: 70.0922 bits (170), Expect = 6.542e-13
Identity = 41/128 (32.03%), Postives = 69/128 (53.91%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI       GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTI------DGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGG 135

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT3G27430.3 (| Symbols: PBB1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr3:10152910-10155052 FORWARD LENGTH=267)

HSP 1 Score: 69.707 bits (169), Expect = 8.545e-13
Identity = 41/128 (32.03%), Postives = 69/128 (53.91%), Query Frame = 1

Query:   94 FSIFVANLPSIMDQVDFNAPHSM--GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKNNLQTGLIVGG 471
            FS  +     ++ Q    AP  +  GTTI       GV+LGAD+R + G  VA++  +KI  +  N+Y C +G+AAD++ V+D V   L  H  Q G+ + V  A  L++   ++ + ++   L++GG
Sbjct:   14 FSFDLCKRNDMLTQKGLKAPSFLKTGTTI------DGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGG 135

[Close]

BLAST of CD859470 vs. TAIR peptide
Match: AT1G13060.1 (| Symbols: PBE1 | 20S proteasome beta subunit E1 | chr1:4452641-4454663 FORWARD LENGTH=274)

HSP 1 Score: 53.9138 bits (128), Expect = 4.852e-8
Identity = 30/108 (27.78%), Postives = 55/108 (50.93%), Query Frame = 1

Query:  154 HSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLAYNNKN-NLQTGLIVGGW 474
            H+ GTT +   + GGV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +      H +   +  +V  A+ L+  + Y+ +   L  G ++ GW
Sbjct:   54 HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGW 161

The following BLAST results are available for this feature:

BLAST of CD859470 vs. TrEMBL
BLAST of CD859470 vs. SwissProt
BLAST of CD859470 vs. TAIR peptide

BLAST of CD859470 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FGZ8_MEDTR	7.255e-60	99.14	Proteasome subunit beta type OS=Medicago truncatul...	[more]
C6SVE5_SOYBN	8.868e-58	97.44	Proteasome subunit beta type OS=Glycine max PE=2 S...	[more]
B4UWD9_ARAHY	2.184e-56	96.46	Threonine endopeptidase (Fragment) OS=Arachis hypo...	[more]
B9SPS6_RICCO	2.045e-54	92.04	Proteasome subunit beta type OS=Ricinus communis G...	[more]
A9PD83_POPTR	1.015e-53	91.96	Proteasome subunit beta type OS=Populus trichocarp...	[more]
A9NLJ2_PICSI	1.015e-53	89.66	Proteasome subunit beta type OS=Picea sitchensis P...	[more]
B9HBA5_POPTR	1.731e-53	87.83	Proteasome subunit beta type OS=Populus trichocarp...	[more]
A9P9E4_POPTR	1.731e-53	87.83	Proteasome subunit beta type OS=Populus trichocarp...	[more]
D7T0M7_VITVI	2.260e-53	92.73	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9NMI9_PICSI	2.952e-53	88.79	Proteasome subunit beta type OS=Picea sitchensis P...	[more]

BLAST of CD859470 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PSB6_TOBAC	4.206e-55	87.93	Proteasome subunit beta type-6 OS=Nicotiana tabacu...	[more]
PSB6_ARATH	1.224e-54	91.15	Proteasome subunit beta type-6 OS=Arabidopsis thal...	[more]
PSB6_DICDI	3.830e-32	58.10	Proteasome subunit beta type-6 OS=Dictyostelium di...	[more]
PSB6_SCHPO	9.763e-28	52.14	Probable proteasome subunit beta type-6 OS=Schizos...	[more]
PSB6_MOUSE	1.275e-27	51.82	Proteasome subunit beta type-6 OS=Mus musculus GN=...	[more]
PSB6_RAT	4.845e-27	50.91	Proteasome subunit beta type-6 OS=Rattus norvegicu...	[more]
PSB6_YEAST	6.328e-27	50.00	Proteasome component PRE3 OS=Saccharomyces cerevis...	[more]
PSB6_BOVIN	6.328e-27	50.91	Proteasome subunit beta type-6 OS=Bos taurus GN=PS...	[more]
PSB6_HUMAN	8.265e-27	51.89	Proteasome subunit beta type-6 OS=Homo sapiens GN=...	[more]
PB6LB_SALSA	6.129e-22	44.86	Proteasome subunit beta type-6-B like protein OS=S...	[more]

BLAST of CD859470 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G31300.3	1.392e-55	91.15	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]
AT4G31300.1	1.392e-55	91.15	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]
AT4G31300.2	2.010e-54	90.35	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]
AT5G40580.2	2.838e-16	32.81	\| Symbols: PBB2 \| 20S proteasome beta subunit PBB2...	[more]
AT5G40580.1	2.838e-16	32.81	\| Symbols: PBB2 \| 20S proteasome beta subunit PBB2...	[more]
AT3G27430.2	3.706e-16	32.81	\| Symbols: PBB1 \| N-terminal nucleophile aminohydr...	[more]
AT3G27430.1	3.706e-16	32.81	\| Symbols: PBB1 \| N-terminal nucleophile aminohydr...	[more]
AT5G40580.3	6.542e-13	32.03	\| Symbols: PBB2 \| 20S proteasome beta subunit PBB2...	[more]
AT3G27430.3	8.545e-13	32.03	\| Symbols: PBB1 \| N-terminal nucleophile aminohydr...	[more]
AT1G13060.1	4.852e-8	27.78	\| Symbols: PBE1 \| 20S proteasome beta subunit E1 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 54..158 score: 7.5
IPR016050	Proteasome, beta-type subunit, conserved site	PROSITE	PS00854	PROTEASOME_B_1	coord: 59..106 score
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 56..158 score: 3
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 28..158 score: 1
None	No IPR available	PANTHER	PTHR11599:SF4	PROTEASOME SUBUNIT BETA TYPE 6,9	coord: 28..158 score: 1
None	No IPR available	PROFILE	PS51476	PROTEASOME_B_2	coord: 55..158 score: 26
None	No IPR available	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 10..158 score: 1.6
None	No IPR available	SEG	seg	seg	coord: 41..50 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859470 ID=CD859470; Name=CD859470; organism=Pisum sativum; type=EST; length=476bp
AAAAACATAGTCCGTAACCCTTTCCCCTTTGCTTCAACACCTTCATTCGA
TTCAGTTCAACCCCAGGCTAGGGCACCGCGTCACACACCTTTCTTCTCCA
TTTTCGTTGCGAATCTACCATCAATCATGGATCAAGTCGATTTCAACGCT
CCCCATTCCATGGGAACCACCATCATCGGTGTTACCTACAACGGCGGAGT
CGTTCTTGGAGCCGACTCTCGAACTAGCACTGGAGTATATGTTGCTAATC
GTGCATCTGATAAAATCACACAGCTCACTGACAATGTCTACGTCTGTCGT
TCTGGATCGGCTGCAGATTCTCAGGTTGTCTCTGACTATGTGCGCTACTT
CCTCCATCAGCACACGATTCAGCTCGGCCAACCTGCCACAGTCAAGGTTG
CTGCTAACCTTGTTCGGCTTCTCGCATATAACAACAAGAATAATTTGCAA
ACCGGGTTAATTGTCGGTGGCTGGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004298	threonine-type endopeptidase activity
GO:0004175	endopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005839	proteasome core complex

Vocabulary: **Biological Process**
Term	Definition
GO:0051603	proteolysis involved in protein catabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001353	Proteasome_sua/b
IPR016050	Proteasome_bsu_CS

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