GH720794

EST Overview
NameGH720794
Unique NameGH720794
TypeEST
OrganismPisum sativum (pea)
Sequence length226
Libraries
Library NameType
LIBEST_024273 BACNIcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of GH720794 vs. TrEMBL
Match: C6T0I3_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.922e-24
Identity = 51/60 (85.00%), Postives = 57/60 (95.00%), Query Frame = -3 
Query:   27 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 206
            S+DD+LEDN +DF NRCQDPN GYAGGPGQMPH+ATTYAAVN+LITLGGEKSLASINR+K
Sbjct:   97 SVDDELEDNAIDFFNRCQDPNGGYAGGPGQMPHIATTYAAVNSLITLGGEKSLASINRDK 156
[Close]
BLAST of GH720794 vs. TrEMBL
Match: D7TWQ3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_66.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032478001 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.735e-22
Identity = 47/60 (78.33%), Postives = 55/60 (91.67%), Query Frame = -3 
Query:   27 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 206
            S+DD+LE+NT+DFL+RCQDPN GY GGPGQMPHLATTYAA+N LITLGG +SL+SINR K
Sbjct:   71 SVDDELENNTIDFLSRCQDPNGGYGGGPGQMPHLATTYAAINALITLGGHRSLSSINRGK 130
[Close]
BLAST of GH720794 vs. TrEMBL
Match: Q6J6P6_CATRO (Farnesyltransferase beta subunit OS=Catharanthus roseus GN=FTB PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.343e-20
Identity = 44/59 (74.58%), Postives = 54/59 (91.53%), Query Frame = -3 
Query:   27 IDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 203
            +D++L +NT+DFL RCQDP+ GY GGPGQ+PHLATTYAAVN+LITLGGEKSL+SI+R K
Sbjct:   88 VDEELANNTIDFLRRCQDPDGGYGGGPGQLPHLATTYAAVNSLITLGGEKSLSSIDRGK 146
[Close]
BLAST of GH720794 vs. TrEMBL
Match: B9RUT5_RICCO (Protein farnesyltransferase beta subunit, putative OS=Ricinus communis GN=RCOM_0850620 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.343e-20
Identity = 44/60 (73.33%), Postives = 54/60 (90.00%), Query Frame = -3 
Query:   27 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 206
            SID +LE+N +DFLNRCQDPN G+ GGPGQ+PHLATTYAAVN+L+TLGG ++L+SINR K
Sbjct:   91 SIDYELENNAIDFLNRCQDPNGGFGGGPGQLPHLATTYAAVNSLVTLGGPRALSSINRGK 150
[Close]
BLAST of GH720794 vs. TrEMBL
Match: A4VCL6_ARATH (At5g40280 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.270e-19
Identity = 42/63 (66.67%), Postives = 52/63 (82.54%), Query Frame = -3 
Query:   18 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNKCTC 206
            ++DD+LE N +DFL RCQ    GY GGPGQ+PHLATTYAAVN L+TLGG+K+L+SINR K +C
Sbjct:  125 TVDDELESNAIDFLGRCQGSEGGYGGGPGQLPHLATTYAAVNALVTLGGDKALSSINREKMSC 187
[Close]
BLAST of GH720794 vs. TrEMBL
Match: Q8LPK8_ARATH (Beta subunit of protein farnesyl transferase ERA1 OS=Arabidopsis thaliana GN=At5g40280 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.830e-19
Identity = 42/63 (66.67%), Postives = 51/63 (80.95%), Query Frame = -3 
Query:   18 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNKCTC 206
            ++DD LE N +DFL RCQ    GY GGPGQ+PHLATTYAAVN L+TLGG+K+L+SINR K +C
Sbjct:   86 TVDDKLESNAIDFLGRCQGSEGGYGGGPGQLPHLATTYAAVNALVTLGGDKALSSINREKMSC 148
[Close]
BLAST of GH720794 vs. TrEMBL
Match: A9NYH3_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.304e-19
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = -3 
Query:   27 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 206
            SID D   +TVDFL RCQDPN GY GGPGQMPHLATTYAAVN+L+T+GG+ +LAS+NR K
Sbjct:   85 SIDADHGQHTVDFLCRCQDPNGGYGGGPGQMPHLATTYAAVNSLVTIGGQTALASVNREK 144
[Close]
BLAST of GH720794 vs. TrEMBL
Match: C5XJ66_SORBI (Putative uncharacterized protein Sb03g034000 OS=Sorghum bicolor GN=Sb03g034000 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.129e-18
Identity = 40/58 (68.97%), Postives = 53/58 (91.38%), Query Frame = -3 
Query:   33 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINR 206
            ++DD+LED+ +DFL RCQD + GY+GGPGQ+PHLATTYAAVNTL+T+G E++L+SINR
Sbjct:  104 ALDDNLEDDIIDFLARCQDKDGGYSGGPGQLPHLATTYAAVNTLVTIGSERALSSINR 161
[Close]
BLAST of GH720794 vs. TrEMBL
Match: B4FPF5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.129e-18
Identity = 40/58 (68.97%), Postives = 53/58 (91.38%), Query Frame = -3 
Query:   33 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINR 206
            ++DDDLE++ +DFL RCQD + GY+GGPGQ+PHLATTYAAVNTL+T+G E++L+SINR
Sbjct:  105 ALDDDLENDIIDFLARCQDKDGGYSGGPGQLPHLATTYAAVNTLVTIGSERALSSINR 162
[Close]
BLAST of GH720794 vs. TrEMBL
Match: Q5JND4_ORYSJ (Putative farnesyltransferase beta subunit OS=Oryza sativa subsp. japonica GN=OSJNBb0021A09.20-1 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.086e-18
Identity = 40/58 (68.97%), Postives = 52/58 (89.66%), Query Frame = -3 
Query:   30 IDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRN 203
            I DD+ED+ VDFL+RCQD + GY GGPGQ+PHLATTYAAVNTL+T+G E++L+S+NR+
Sbjct:  112 IPDDVEDDIVDFLSRCQDKDGGYGGGPGQLPHLATTYAAVNTLVTIGSERALSSVNRD 169
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_PEA (Protein farnesyltransferase subunit beta OS=Pisum sativum GN=FTB PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.401e-28
Identity = 59/60 (98.33%), Postives = 59/60 (98.33%), Query Frame = -3 
Query:   27 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 206
            SIDDDLEDNTVDFLNRCQDPN GYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK
Sbjct:   62 SIDDDLEDNTVDFLNRCQDPNGGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 121
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_ARATH (Protein farnesyltransferase subunit beta OS=Arabidopsis thaliana GN=FTB PE=1 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 6.302e-21
Identity = 42/63 (66.67%), Postives = 52/63 (82.54%), Query Frame = -3 
Query:   18 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNKCTC 206
            ++DD+LE N +DFL RCQ    GY GGPGQ+PHLATTYAAVN L+TLGG+K+L+SINR K +C
Sbjct:   47 TVDDELESNAIDFLGRCQGSEGGYGGGPGQLPHLATTYAAVNALVTLGGDKALSSINREKMSC 109
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_RAT (Protein farnesyltransferase subunit beta OS=Rattus norvegicus GN=Fntb PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 8.537e-10
Identity = 28/48 (58.33%), Postives = 33/48 (68.75%), Query Frame = -3 
Query:   27 FLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 170
            FL  CQ P+ G+ GGPGQ PHLA TYAAVN L  +G E++   INR K
Sbjct:  129 FLELCQSPDGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYNVINREK 176
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_MOUSE (Protein farnesyltransferase subunit beta OS=Mus musculus GN=Fntb PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 8.537e-10
Identity = 28/48 (58.33%), Postives = 33/48 (68.75%), Query Frame = -3 
Query:   27 FLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 170
            FL  CQ P+ G+ GGPGQ PHLA TYAAVN L  +G E++   INR K
Sbjct:  129 FLELCQSPDGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYNVINREK 176
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_HUMAN (Protein farnesyltransferase subunit beta OS=Homo sapiens GN=FNTB PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.115e-9
Identity = 28/48 (58.33%), Postives = 32/48 (66.67%), Query Frame = -3 
Query:   27 FLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 170
            FL  CQ P  G+ GGPGQ PHLA TYAAVN L  +G E++   INR K
Sbjct:  129 FLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREK 176
[Close]
BLAST of GH720794 vs. SwissProt
Match: FNTB_BOVIN (Protein farnesyltransferase subunit beta OS=Bos taurus GN=FNTB PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.115e-9
Identity = 28/48 (58.33%), Postives = 32/48 (66.67%), Query Frame = -3 
Query:   27 FLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNK 170
            FL  CQ P  G+ GGPGQ PHLA TYAAVN L  +G E++   INR K
Sbjct:  129 FLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDVINREK 176
[Close]
BLAST of GH720794 vs. TAIR peptide
Match: AT5G40280.1 (| Symbols: ERA1, ATFTB, WIG | Prenyltransferase family protein | chr5:16101391-16104783 FORWARD LENGTH=482)

HSP 1 Score: 99.3673 bits (246), Expect = 4.892e-22
Identity = 42/63 (66.67%), Postives = 52/63 (82.54%), Query Frame = -3 
Query:   18 SIDDDLEDNTVDFLNRCQDPNAGYAGGPGQMPHLATTYAAVNTLITLGGEKSLASINRNKCTC 206
            ++DD+LE N +DFL RCQ    GY GGPGQ+PHLATTYAAVN L+TLGG+K+L+SINR K +C
Sbjct:  125 TVDDELESNAIDFLGRCQGSEGGYGGGPGQLPHLATTYAAVNALVTLGGDKALSSINREKMSC 187
The following BLAST results are available for this feature:
BLAST of GH720794 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6T0I3_SOYBN2.922e-2485.00Putative uncharacterized protein (Fragment) OS=Gly... [more]
D7TWQ3_VITVI2.735e-2278.33Whole genome shotgun sequence of line PN40024, sca... [more]
Q6J6P6_CATRO3.343e-2074.58Farnesyltransferase beta subunit OS=Catharanthus r... [more]
B9RUT5_RICCO3.343e-2073.33Protein farnesyltransferase beta subunit, putative... [more]
A4VCL6_ARATH1.270e-1966.67At5g40280 OS=Arabidopsis thaliana PE=2 SV=1[more]
Q8LPK8_ARATH2.830e-1966.67Beta subunit of protein farnesyl transferase ERA1 ... [more]
A9NYH3_PICSI6.304e-1973.33Putative uncharacterized protein OS=Picea sitchens... [more]
C5XJ66_SORBI3.129e-1868.97Putative uncharacterized protein Sb03g034000 OS=So... [more]
B4FPF5_MAIZE3.129e-1868.97Putative uncharacterized protein OS=Zea mays PE=2 ... [more]
Q5JND4_ORYSJ4.086e-1868.97Putative farnesyltransferase beta subunit OS=Oryza... [more]
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BLAST of GH720794 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 6
Match NameE-valueIdentityDescription
FNTB_PEA1.401e-2898.33Protein farnesyltransferase subunit beta OS=Pisum ... [more]
FNTB_ARATH6.302e-2166.67Protein farnesyltransferase subunit beta OS=Arabid... [more]
FNTB_RAT8.537e-1058.33Protein farnesyltransferase subunit beta OS=Rattus... [more]
FNTB_MOUSE8.537e-1058.33Protein farnesyltransferase subunit beta OS=Mus mu... [more]
FNTB_HUMAN1.115e-958.33Protein farnesyltransferase subunit beta OS=Homo s... [more]
FNTB_BOVIN1.115e-958.33Protein farnesyltransferase subunit beta OS=Bos ta... [more]
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BLAST of GH720794 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G40280.14.892e-2266.67| Symbols: ERA1, ATFTB, WIG | Prenyltransferase fa... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 39..53
score
NoneNo IPR availableGENE3D1.50.10.20no descriptioncoord: 7..67
score: 1.2
NoneNo IPR availablePANTHERPTHR11774GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNITcoord: 10..66
score: 9.2
NoneNo IPR availablePANTHERPTHR11774:SF6PROTEIN FARNESYLTRANSFERASE BETA SUBUNIT (CAAX FARNESYLTRANSFERASE BETA SUBUNIT) (RAS PROTEINS PRENYLTRANSFERASE BETA) (FTASE-BETA)coord: 10..66
score: 9.2
IPR001330Prenyltransferase/squalene oxidasePFAMPF00432Prenyltranscoord: 15..54
score: 5
IPR008930Terpenoid cylases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 8..67
score: 2.9

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH720794 ID=GH720794; Name=GH720794; organism=Pisum sativum; type=EST; length=226bp
TTCGAGCGGCCGCCCGGGCAGGTACACTTATTTCTATTAATAGATGCCAA
AGATTTCTCACCACCCAGAGTAATAAGAGTATTGACTGCAGCATAAGTTG
TGGCAAGATGAGGCATCTGACCAGGTCCCCCAGCATATCCAGCATTTGGA
TCCTGGCAACGGTTAAGAAAATCGACAGTGTTATCTTCGAGATCATCATC
AATAGATACCTCGGCCGCGACCACGC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR001330PFTB_repeat
IPR008930Terpenoid_cyclase/PrenylTrfase