EX570008

EST Overview


Name	EX570008
Unique Name	EX570008
Type	EST
Organism	Pisum sativum (pea)
Sequence length	254

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570008 vs. TrEMBL
Match: B9HD09_POPTR (F-box family protein OS=Populus trichocarpa GN=POPTRDRAFT_865582 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 5.708e-30
Identity = 40/49 (81.63%), Postives = 46/49 (93.88%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            P+L++LDLSYTFL+NLQPVFDSC QLKVLKLQACK+LTD+SLEP YK G
Sbjct:  706 PHLSVLDLSYTFLMNLQPVFDSCLQLKVLKLQACKYLTDTSLEPLYKDG 754

HSP 2 Score: 67.781 bits (164), Expect = 5.708e-30
Identity = 31/38 (81.58%), Postives = 34/38 (89.47%), Query Frame = 2

Query:  140 RGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            + GALPALQELDLSYGTLCQ AIEELL+CC HLT +SL
Sbjct:  752 KDGALPALQELDLSYGTLCQSAIEELLACCRHLTHLSL 789

[Close]

BLAST of EX570008 vs. TrEMBL
Match: B9IKE4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1105729 PE=4 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 7.426e-30
Identity = 40/49 (81.63%), Postives = 45/49 (91.84%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            P+L LLDLSYTFL+NL+PVFDSC QLKVLKLQACK+LTD+SLEP YK G
Sbjct:  702 PHLTLLDLSYTFLMNLEPVFDSCLQLKVLKLQACKYLTDTSLEPLYKDG 750

HSP 2 Score: 67.781 bits (164), Expect = 7.426e-30
Identity = 31/38 (81.58%), Postives = 34/38 (89.47%), Query Frame = 2

Query:  140 RGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            + GALPALQELDLSYGTLCQ AIEELL+CC HLT +SL
Sbjct:  748 KDGALPALQELDLSYGTLCQSAIEELLACCRHLTHLSL 785

[Close]

BLAST of EX570008 vs. TrEMBL
Match: D7M986_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_656701 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.132e-29
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            PNL +LDLSYTFL+NL+PVF SC QLKVLKLQACK+LTDSSLEP YK G
Sbjct:  735 PNLTVLDLSYTFLMNLEPVFKSCVQLKVLKLQACKYLTDSSLEPLYKEG 783

HSP 2 Score: 65.855 bits (159), Expect = 6.132e-29
Identity = 29/36 (80.56%), Postives = 34/36 (94.44%), Query Frame = 2

Query:  146 GALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            GALPAL+ELDLSYGTLCQ AI++LL+CCTHLT +SL
Sbjct:  783 GALPALEELDLSYGTLCQTAIDDLLACCTHLTHLSL 818

[Close]

BLAST of EX570008 vs. TrEMBL
Match: D7STX3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035469001 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.354e-28
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            P+L LLDLSYTFL+NLQPVF+SC QLKVLKLQACK+LTDSSLE  YK G
Sbjct:  702 PHLTLLDLSYTFLMNLQPVFESCLQLKVLKLQACKYLTDSSLEALYKEG 750

HSP 2 Score: 66.2402 bits (160), Expect = 1.354e-28
Identity = 32/43 (74.42%), Postives = 34/43 (79.07%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L    + GALPAL ELDLSYG LCQ AIEELL+CCTHLT VSL
Sbjct:  743 LEALYKEGALPALCELDLSYGALCQSAIEELLACCTHLTHVSL 785

[Close]

BLAST of EX570008 vs. TrEMBL
Match: B9RQW5_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0707280 PE=4 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 6.590e-28
Identity = 40/49 (81.63%), Postives = 45/49 (91.84%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            PNL +LDLSYTFL+NLQPVF+SC QLKVLKLQACK+LTD+SLEP YK G
Sbjct:  739 PNLTVLDLSYTFLMNLQPVFESCLQLKVLKLQACKYLTDTSLEPLYKEG 787

HSP 2 Score: 61.2326 bits (147), Expect = 6.590e-28
Identity = 29/36 (80.56%), Postives = 31/36 (86.11%), Query Frame = 2

Query:  146 GALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            GALP LQ LDLSYGTLCQ AIEELL+ CTHLT +SL
Sbjct:  787 GALPVLQVLDLSYGTLCQSAIEELLAYCTHLTHLSL 822

[Close]

BLAST of EX570008 vs. TrEMBL
Match: Q8S5N8_ORYSJ (Putative F-box protein family OS=Oryza sativa subsp. japonica GN=OSJNBb0058B20.28 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.211e-22
Identity = 35/47 (74.47%), Postives = 41/47 (87.23%), Query Frame = 1

Query:    7 LALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            LALLDLSYTFL NL+PVFDSC QLK+LKL ACK+L+DSSL+  Y+ G
Sbjct:  757 LALLDLSYTFLTNLKPVFDSCPQLKILKLSACKYLSDSSLDALYREG 803

HSP 2 Score: 53.9138 bits (128), Expect = 1.211e-22
Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L+   R GALP L ELDLSY ++ Q AIEELLSCCT+L  V+L
Sbjct:  796 LDALYREGALPMLVELDLSYSSIGQTAIEELLSCCTNLVNVNL 838

[Close]

BLAST of EX570008 vs. TrEMBL
Match: B8BGP0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_33456 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.211e-22
Identity = 35/47 (74.47%), Postives = 41/47 (87.23%), Query Frame = 1

Query:    7 LALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            LALLDLSYTFL NL+PVFDSC QLK+LKL ACK+L+DSSL+  Y+ G
Sbjct:  697 LALLDLSYTFLTNLKPVFDSCPQLKILKLSACKYLSDSSLDALYREG 743

HSP 2 Score: 53.9138 bits (128), Expect = 1.211e-22
Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L+   R GALP L ELDLSY ++ Q AIEELLSCCT+L  V+L
Sbjct:  736 LDALYREGALPMLVELDLSYSSIGQTAIEELLSCCTNLVNVNL 778

[Close]

BLAST of EX570008 vs. TrEMBL
Match: B9G5L4_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_31423 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.213e-22
Identity = 35/47 (74.47%), Postives = 41/47 (87.23%), Query Frame = 1

Query:    7 LALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            LALLDLSYTFL NL+PVFDSC QLK+LKL ACK+L+DSSL+  Y+ G
Sbjct:  757 LALLDLSYTFLTNLKPVFDSCPQLKILKLSACKYLSDSSLDALYREG 803

HSP 2 Score: 53.9138 bits (128), Expect = 1.213e-22
Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L+   R GALP L ELDLSY ++ Q AIEELLSCCT+L  V+L
Sbjct:  796 LDALYREGALPMLVELDLSYSSIGQTAIEELLSCCTNLVNVNL 838

[Close]

BLAST of EX570008 vs. TrEMBL
Match: Q338L7_ORYSJ (F-box family protein, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os10g25680 PE=4 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 1.214e-22
Identity = 35/47 (74.47%), Postives = 41/47 (87.23%), Query Frame = 1

Query:    7 LALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            LALLDLSYTFL NL+PVFDSC QLK+LKL ACK+L+DSSL+  Y+ G
Sbjct:  697 LALLDLSYTFLTNLKPVFDSCPQLKILKLSACKYLSDSSLDALYREG 743

HSP 2 Score: 53.9138 bits (128), Expect = 1.214e-22
Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L+   R GALP L ELDLSY ++ Q AIEELLSCCT+L  V+L
Sbjct:  736 LDALYREGALPMLVELDLSYSSIGQTAIEELLSCCTNLVNVNL 778

[Close]

BLAST of EX570008 vs. TrEMBL
Match: Q0IXV2_ORYSJ (Os10g0396400 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os10g0396400 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.221e-22
Identity = 35/47 (74.47%), Postives = 41/47 (87.23%), Query Frame = 1

Query:    7 LALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            LALLDLSYTFL NL+PVFDSC QLK+LKL ACK+L+DSSL+  Y+ G
Sbjct:  399 LALLDLSYTFLTNLKPVFDSCPQLKILKLSACKYLSDSSLDALYREG 445

HSP 2 Score: 53.9138 bits (128), Expect = 1.221e-22
Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  125 LNLFTRGGALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            L+   R GALP L ELDLSY ++ Q AIEELLSCCT+L  V+L
Sbjct:  438 LDALYREGALPMLVELDLSYSSIGQTAIEELLSCCTNLVNVNL 480

[Close]

BLAST of EX570008 vs. SwissProt
Match: FBL15_ARATH (F-box/LRR-repeat protein 15 OS=Arabidopsis thaliana GN=FBL15 PE=1 SV=2)

HSP 1 Score: 85.5001 bits (210), Expect = 2.942e-30
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            PNL +LDLSYTFL+NL+PVF SC QLKVLKLQACK+LTDSSLEP YK G
Sbjct:  735 PNLTVLDLSYTFLMNLEPVFKSCIQLKVLKLQACKYLTDSSLEPLYKEG 783

HSP 2 Score: 65.855 bits (159), Expect = 2.942e-30
Identity = 29/36 (80.56%), Postives = 34/36 (94.44%), Query Frame = 2

Query:  146 GALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            GALPAL+ELDLSYGTLCQ AI++LL+CCTHLT +SL
Sbjct:  783 GALPALEELDLSYGTLCQTAIDDLLACCTHLTHLSL 818

[Close]

BLAST of EX570008 vs. TAIR peptide
Match: AT4G33210.1 (| Symbols: SLOMO | F-box family protein | chr4:16015971-16020697 REVERSE LENGTH=990)

HSP 1 Score: 85.5001 bits (210), Expect = 2.384e-31
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 1

Query:    1 PNLALLDLSYTFLVNLQPVFDSCSQLKVLKLQACKFLTDSSLEPFYKGG 147
            PNL +LDLSYTFL+NL+PVF SC QLKVLKLQACK+LTDSSLEP YK G
Sbjct:  735 PNLTVLDLSYTFLMNLEPVFKSCIQLKVLKLQACKYLTDSSLEPLYKEG 783

HSP 2 Score: 65.855 bits (159), Expect = 2.384e-31
Identity = 29/36 (80.56%), Postives = 34/36 (94.44%), Query Frame = 2

Query:  146 GALPALQELDLSYGTLCQKAIEELLSCCTHLTRVSL 253
            GALPAL+ELDLSYGTLCQ AI++LL+CCTHLT +SL
Sbjct:  783 GALPALEELDLSYGTLCQTAIDDLLACCTHLTHLSL 818

The following BLAST results are available for this feature:

BLAST of EX570008 vs. TrEMBL
BLAST of EX570008 vs. SwissProt
BLAST of EX570008 vs. TAIR peptide

BLAST of EX570008 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HD09_POPTR	5.708e-30	81.63	F-box family protein OS=Populus trichocarpa GN=POP...	[more]
B9IKE4_POPTR	7.426e-30	81.63	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7M986_ARALY	6.132e-29	81.63	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
D7STX3_VITVI	1.354e-28	81.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RQW5_RICCO	6.590e-28	81.63	Putative uncharacterized protein OS=Ricinus commun...	[more]
Q8S5N8_ORYSJ	1.211e-22	74.47	Putative F-box protein family OS=Oryza sativa subs...	[more]
B8BGP0_ORYSI	1.211e-22	74.47	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B9G5L4_ORYSJ	1.213e-22	74.47	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q338L7_ORYSJ	1.214e-22	74.47	F-box family protein, putative, expressed OS=Oryza...	[more]
Q0IXV2_ORYSJ	1.221e-22	74.47	Os10g0396400 protein (Fragment) OS=Oryza sativa su...	[more]

BLAST of EX570008 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
FBL15_ARATH	2.942e-30	81.63	F-box/LRR-repeat protein 15 OS=Arabidopsis thalian...	[more]

BLAST of EX570008 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G33210.1	2.384e-31	81.63	\| Symbols: SLOMO \| F-box family protein \| chr4:160...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.80.10.10	no description	coord: 2..43 score: 0.0
None	No IPR available	PANTHER	PTHR23125	F-BOX/LEUCINE RICH REPEAT PROTEIN	coord: 1..48 score: 2.1
None	No IPR available	PANTHER	PTHR23125:SF57	gb def: Hypothetical protein F4I10.140 (Hypothetical protein AT4g33210)	coord: 1..48 score: 2.1
None	No IPR available	PANTHER	PTHR23125	F-BOX/LEUCINE RICH REPEAT PROTEIN	coord: 20..55 score: 1.6
None	No IPR available	PANTHER	PTHR23125:SF57	gb def: Hypothetical protein F4I10.140 (Hypothetical protein AT4g33210)	coord: 20..55 score: 1.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570008 ID=EX570008; Name=EX570008; organism=Pisum sativum; type=EST; length=254bp
CCAAATTTAGCTCTTCTTGACTTATCATACACTTTCTTGGTGAATCTGCA
GCCTGTTTTTGACTCTTGTTCACAGCTAAAGGTATTAAAGTTGCAGGCAT
GCAAATTTCTCACTGACTCATCACTTGAACCTTTTTACAAGGGGGGGTGC
TTTACCAGCTCTTCAGGAGTTGGACTTGTCTTATGGAACTTTGTGTCAGA
AGGCCATAGAAGAGCTTCTTTCTTGCTGCACTCACCTTACACGTGTGAGT
TTGA

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