EX570119

EST Overview


Name	EX570119
Unique Name	EX570119
Type	EST
Organism	Pisum sativum (pea)
Sequence length	343

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570119 vs. TrEMBL
Match: Q39856_SOYBN (Epoxide hydrolase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.965e-17
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = -1

Query:    2 ELEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 169
            E+E I HRTV  NGI +H+AEKG+GP++LF+ GFP+LWYSWRHQI  LSS GYR V
Sbjct:   25 EMEQIKHRTVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWRHQILSLSSLGYRAV 80

[Close]

BLAST of EX570119 vs. TrEMBL
Match: O49857_SOYBN (Epoxide hydrolase OS=Glycine max PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.965e-17
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = -1

Query:    2 ELEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 169
            E+E I HRTV  NGI +H+AEKG+GP++LF+ GFP+LWYSWRHQI  LSS GYR V
Sbjct:   25 EMEQIKHRTVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWRHQILSLSSLGYRAV 80

[Close]

BLAST of EX570119 vs. TrEMBL
Match: C6TK81_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.700e-16
Identity = 37/55 (67.27%), Postives = 46/55 (83.64%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E ++HRTV  NGI +HIAEKG+GP++LF+ GFP+LWYSWRHQI  LSS GYR V
Sbjct:    1 MEGVIHRTVEVNGIKMHIAEKGEGPVVLFLHGFPELWYSWRHQILSLSSLGYRAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: C6T8Y6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.945e-16
Identity = 36/55 (65.45%), Postives = 46/55 (83.64%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I+HRTV  NGI +H+AEKG+GP++LF+ GFP+LWYSWRHQI  LS+ GYR V
Sbjct:    1 MEGIVHRTVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWRHQILALSNLGYRAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: D7SYB5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_77.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035113001 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.459e-16
Identity = 38/55 (69.09%), Postives = 45/55 (81.82%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E + HRTV ANGINIH+AEKG+GP+ILFI GFP+ WYSWRHQI  L+  GYR V
Sbjct:    1 MEGVEHRTVKANGINIHVAEKGEGPIILFIHGFPESWYSWRHQIHALALLGYRAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: B9RFD7_RICCO (Epoxide hydrolase, putative OS=Ricinus communis GN=RCOM_1433900 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.102e-15
Identity = 36/55 (65.45%), Postives = 46/55 (83.64%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I+H+TV  NGI +HIAEKG+GP+ILF+ GFP+LWY+WRHQI  L+S GYR V
Sbjct:    1 MEGIMHKTVNVNGIKMHIAEKGEGPVILFLHGFPELWYTWRHQILGLTSLGYRAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: D7U9X4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031167001 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.439e-15
Identity = 34/55 (61.82%), Postives = 45/55 (81.82%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I HR V+ NGI +H+AEKGQGP++LF+ GFP+LWY+WRHQI  ++SHGY  V
Sbjct:    1 MEGIEHRMVSVNGIKMHVAEKGQGPVVLFLHGFPELWYTWRHQIIAMASHGYHAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: C6TC98_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.439e-15
Identity = 36/55 (65.45%), Postives = 44/55 (80.00%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I HRTV  NGI +H+AEKG+GP++LF+ GFP+LWYSW HQI  LSS GYR V
Sbjct:    1 MEQIKHRTVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWHHQILSLSSLGYRAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: A5BIA2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_022460 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.439e-15
Identity = 34/55 (61.82%), Postives = 45/55 (81.82%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I HR V+ NGI +H+AEKGQGP++LF+ GFP+LWY+WRHQI  ++SHGY  V
Sbjct:    1 MEGIEHRMVSVNGIKMHVAEKGQGPVVLFLHGFPELWYTWRHQIIAMASHGYHAV 55

[Close]

BLAST of EX570119 vs. TrEMBL
Match: C6TCC6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.879e-15
Identity = 36/55 (65.45%), Postives = 44/55 (80.00%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I HR V  NGI +H+AEKG+GP++LF+ GFP+LWYSWRHQI  LSS GYR V
Sbjct:    1 MEQIKHRIVEVNGIKMHVAEKGEGPVVLFLHGFPELWYSWRHQILSLSSLGYRAV 55

[Close]

BLAST of EX570119 vs. SwissProt
Match: YFHM_BACSU (AB hydrolase superfamily protein yfhM OS=Bacillus subtilis GN=yfhM PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.728e-7
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = -1

Query:    2 VTANGINIHIAEKGQ--GPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 142
            V  NGI +H+A  G+  GPLI+ + GFP+ WY W++QI  L   GYR +
Sbjct:    9 VNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVI 57

[Close]

BLAST of EX570119 vs. SwissProt
Match: HYES_RAT (Epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 8.577e-7
Identity = 23/51 (45.10%), Postives = 30/51 (58.82%), Query Frame = -1

Query:    8 ILHRTVTAN-GINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYR 157
            + H  VT   GI +H  E G GP I    GFP+ W+SWR+QI  L+  G+R
Sbjct:  235 VSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFR 285

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT4G02340.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:1035722-1037403 FORWARD LENGTH=324)

HSP 1 Score: 79.7221 bits (195), Expect = 3.988e-16
Identity = 31/55 (56.36%), Postives = 42/55 (76.36%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKGQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I H T++ NGIN+H+A  G GP+ILF+ GFPDLWYSWRHQ+   ++ GYR +
Sbjct:    1 MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAI 55

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT3G05600.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:1623485-1624704 REVERSE LENGTH=331)

HSP 1 Score: 78.9518 bits (193), Expect = 6.803e-16
Identity = 36/57 (63.16%), Postives = 45/57 (78.95%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEKG--QGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            +E I HR V+ NGI +HIAEKG  +GP++L + GFPDLWY+WRHQI+ LSS GYR V
Sbjct:    1 MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAV 57

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT4G15960.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9045763-9047199 REVERSE LENGTH=375)

HSP 1 Score: 75.485 bits (184), Expect = 7.522e-15
Identity = 32/60 (53.33%), Postives = 45/60 (75.00%), Query Frame = -1

Query:    2 LEPILHRTVTANGINIHIAEK-----GQGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 166
            L+ + H+T+  NGIN+H+AEK     G+ P+ILF+ GFP+LWY+WRHQ+  LSS GYR +
Sbjct:   51 LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT3G51000.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:18945258-18946499 REVERSE LENGTH=323)

HSP 1 Score: 71.2478 bits (173), Expect = 1.419e-13
Identity = 30/54 (55.56%), Postives = 40/54 (74.07%), Query Frame = -1

Query:    2 ILHRTVTANGINIHIAEKG--QGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 157
            +  + +  NGI +++AEKG  +GPL+L + GFP+ WYSWRHQI FLSSHGY  V
Sbjct:    5 VREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVV 58

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT2G26740.1 (| Symbols: ATSEH, SEH | soluble epoxide hydrolase | chr2:11393148-11394257 REVERSE LENGTH=321)

HSP 1 Score: 70.0922 bits (170), Expect = 3.160e-13
Identity = 30/52 (57.69%), Postives = 39/52 (75.00%), Query Frame = -1

Query:    2 HRTVTANGINIHIAEKG--QGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 151
            HR V  NGI+IH+A +G   GP++L + GFP+LWYSWRHQI  L++ GYR V
Sbjct:    3 HRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAV 54

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT2G26750.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr2:11395971-11397085 REVERSE LENGTH=320)

HSP 1 Score: 68.1662 bits (165), Expect = 1.201e-12
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = -1

Query:    2 HRTVTANGINIHIAEKG--QGPLILFIRGFPDLWYSWRHQITFLSSHGYRCV 151
            HR V  NGI+IH+A +G   G ++L + GFP+LWYSWRHQI+ L++ GYR V
Sbjct:    3 HRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAV 54

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT4G15955.3 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9043872-9045544 REVERSE LENGTH=304)

HSP 1 Score: 64.6994 bits (156), Expect = 1.328e-11
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = -1

Query:    2 HRTVTANGINIHIAEK-----GQG----PLILFIRGFPDLWYSWRHQITFLSSHGYRCV 151
            H  V  NGI +H+AEK     G G    P+ILF+ GFP+LWY+WRHQ+  LSS GYR +
Sbjct:    7 HSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTI 65

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT4G15955.2 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9044649-9045544 REVERSE LENGTH=183)

HSP 1 Score: 64.6994 bits (156), Expect = 1.328e-11
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = -1

Query:    2 HRTVTANGINIHIAEK-----GQG----PLILFIRGFPDLWYSWRHQITFLSSHGYRCV 151
            H  V  NGI +H+AEK     G G    P+ILF+ GFP+LWY+WRHQ+  LSS GYR +
Sbjct:    7 HSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTI 65

[Close]

BLAST of EX570119 vs. TAIR peptide
Match: AT4G15955.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9044083-9045544 REVERSE LENGTH=178)

HSP 1 Score: 64.6994 bits (156), Expect = 1.328e-11
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = -1

Query:    2 HRTVTANGINIHIAEK-----GQG----PLILFIRGFPDLWYSWRHQITFLSSHGYRCV 151
            H  V  NGI +H+AEK     G G    P+ILF+ GFP+LWY+WRHQ+  LSS GYR +
Sbjct:    7 HSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTI 65

The following BLAST results are available for this feature:

BLAST of EX570119 vs. TrEMBL
BLAST of EX570119 vs. SwissProt
BLAST of EX570119 vs. TAIR peptide

BLAST of EX570119 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q39856_SOYBN	9.965e-17	67.86	Epoxide hydrolase OS=Glycine max PE=2 SV=1	[more]
O49857_SOYBN	9.965e-17	67.86	Epoxide hydrolase OS=Glycine max PE=4 SV=1	[more]
C6TK81_SOYBN	1.700e-16	67.27	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T8Y6_SOYBN	4.945e-16	65.45	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7SYB5_VITVI	6.459e-16	69.09	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RFD7_RICCO	1.102e-15	65.45	Epoxide hydrolase, putative OS=Ricinus communis GN...	[more]
D7U9X4_VITVI	1.439e-15	61.82	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6TC98_SOYBN	1.439e-15	65.45	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
A5BIA2_VITVI	1.439e-15	61.82	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C6TCC6_SOYBN	1.879e-15	65.45	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of EX570119 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
YFHM_BACSU	1.728e-7	46.94	AB hydrolase superfamily protein yfhM OS=Bacillus ...	[more]
HYES_RAT	8.577e-7	45.10	Epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 ...	[more]

BLAST of EX570119 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT4G02340.1	3.988e-16	56.36	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G05600.1	6.803e-16	63.16	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15960.1	7.522e-15	53.33	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT3G51000.1	1.419e-13	55.56	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT2G26740.1	3.160e-13	57.69	\| Symbols: ATSEH, SEH \| soluble epoxide hydrolase ...	[more]
AT2G26750.1	1.201e-12	55.77	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15955.3	1.328e-11	52.54	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15955.2	1.328e-11	52.54	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]
AT4G15955.1	1.328e-11	52.54	\| Symbols: \| alpha/beta-Hydrolases superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 65..83 score: 6.1e-05coord: 85..89 score: 6.1
None	No IPR available	GENE3D	3.40.50.1820	no description	coord: 30..89 score: 1.9
None	No IPR available	PANTHER	PTHR10992	ALPHA/BETA HYDROLASE RELATED	coord: 47..89 score: 1.1
None	No IPR available	PANTHER	PTHR10992:SF22	EPOXIDE HYDROLASE	coord: 47..89 score: 1.1
None	No IPR available	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 33..89 score: 5.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570119 ID=EX570119; Name=EX570119; organism=Pisum sativum; type=EST; length=343bp
ACACACAGCGGTAGCCGTGGGAAGACAGAAAAGTGATCTGGTGGCGCCAT
GAATACCATAGGTCGGGGAAACCACGGATGAAGAGGATTAATGGACCTTG
ACCCTTTTCAGCTATGTGTATGTTTATGCCATTAGCTGTTACTGTTCGGT
GAAGTATAGGTTCTAGTTCCATGTTACTGTCGGAATGTGGTGAGTGGTGG
AGAATGTTTATGAGCGTTTGTGTGGCTTTGTTCCTGCTCTGATTGCATCT
TTTGTGGTTATGAATTCCTCAAGCTTGGGTCTTATTAAGTGTGATATGAA
TAATGTCATTGTACATGTTTATGAACTCTTATGCTCGAGTACT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000639	Epox_hydrolase-like

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