EX570370

EST Overview


Name	EX570370
Unique Name	EX570370
Type	EST
Organism	Pisum sativum (pea)
Sequence length	479

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570370 vs. TrEMBL
Match: Q9ZRY9_CICAR (20S proteasome beta subunit OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 5.366e-47
Identity = 90/93 (96.77%), Postives = 91/93 (97.85%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAGP 280
            RFSVGSGSPYAYGVLDSGYKYDMS+EEAS LARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEA GP
Sbjct:  146 RFSVGSGSPYAYGVLDSGYKYDMSIEEASELARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEATGP 238

[Close]

BLAST of EX570370 vs. TrEMBL
Match: C6TLJ9_SOYBN (Proteasome subunit beta type OS=Glycine max PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.472e-44
Identity = 85/92 (92.39%), Postives = 88/92 (95.65%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAG 277
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ LARRAIYHATFRDGASGGVASVYYVGP GWKKLSGDDVGELHYHY PVTPSTVEQEMVEA G
Sbjct:  180 RFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYHYYPVTPSTVEQEMVEATG 271

[Close]

BLAST of EX570370 vs. TrEMBL
Match: C3VIB9_CITMA (20S proteasome beta subunit 5 (Fragment) OS=Citrus maxima PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.047e-42
Identity = 83/92 (90.22%), Postives = 86/92 (93.48%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAG 277
            RFSVGSGSPYAYGVLDSGYK+DMS+EEA+ LARRAIYHATFRD ASGGVASVYYVGP GWKKLSGDDVGELHYHY PVTPSTVEQEM E AG
Sbjct:  102 RFSVGSGSPYAYGVLDSGYKFDMSIEEAAELARRAIYHATFRDAASGGVASVYYVGPNGWKKLSGDDVGELHYHYYPVTPSTVEQEMAEVAG 193

[Close]

BLAST of EX570370 vs. TrEMBL
Match: C3VIB8_CITMA (Proteasome subunit beta type OS=Citrus maxima PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.047e-42
Identity = 83/92 (90.22%), Postives = 86/92 (93.48%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAG 277
            RFSVGSGSPYAYGVLDSGYK+DMS+EEA+ LARRAIYHATFRD ASGGVASVYYVGP GWKKLSGDDVGELHYHY PVTPSTVEQEM E AG
Sbjct:  180 RFSVGSGSPYAYGVLDSGYKFDMSIEEAAELARRAIYHATFRDAASGGVASVYYVGPNGWKKLSGDDVGELHYHYYPVTPSTVEQEMAEVAG 271

[Close]

BLAST of EX570370 vs. TrEMBL
Match: D7TAR8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_10.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010375001 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.158e-41
Identity = 82/92 (89.13%), Postives = 86/92 (93.48%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAG 277
            RFSVGSGSPYAYGVLD+GY+YDMSVEEA+ LARR+IYHATFRDGASGGVASVYYVGP GWKKLSGDDVGELHY Y PV PSTVEQEMVE AG
Sbjct:  180 RFSVGSGSPYAYGVLDNGYRYDMSVEEAAELARRSIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYKYYPVMPSTVEQEMVEVAG 271

[Close]

BLAST of EX570370 vs. TrEMBL
Match: B7FKR0_MEDTR (Putative uncharacterized protein (Fragment) OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 9.802e-41
Identity = 82/84 (97.62%), Postives = 82/84 (97.62%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVE 253
            RFSVGSGSPYAYGVLDSGYKYDMSVEEAS LARRAIYHATFRDGASGGVASVYYVGPTG KKLSGDDVGELHYHYNPVTPSTVE
Sbjct:  179 RFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFRDGASGGVASVYYVGPTGRKKLSGDDVGELHYHYNPVTPSTVE 262

[Close]

BLAST of EX570370 vs. TrEMBL
Match: Q93WZ4_PRUPE (Putative 20S proteasome beta subunit 5 (Fragment) OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 8.298e-40
Identity = 79/91 (86.81%), Postives = 82/91 (90.11%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAA 274
            RFSVGSGSPYAYGVLD GY+YDMSVEEA+ LARRAIYHATFRDGASGGVASVY+VGP GWKKLSGDDVGELHYHY PV    VEQEMVE A
Sbjct:  109 RFSVGSGSPYAYGVLDDGYRYDMSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGWKKLSGDDVGELHYHYYPVVEGAVEQEMVEVA 199

[Close]

BLAST of EX570370 vs. TrEMBL
Match: D7LR41_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_484397 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 8.298e-40
Identity = 80/91 (87.91%), Postives = 83/91 (91.21%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAA 274
            RFSVGSGSPYAYGVLDSGYK+DMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GWKKLSGDDVGELHYHY PVTP T E  M EAA
Sbjct:  183 RFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQGWKKLSGDDVGELHYHYYPVTPVTAEHVMEEAA 273

[Close]

BLAST of EX570370 vs. TrEMBL
Match: D7KPF3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471459 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 9.175e-39
Identity = 78/90 (86.67%), Postives = 81/90 (90.00%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEA 271
            RFSVGSGSPYAYGVLDSGYKYDMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P+  EQ M EA
Sbjct:  182 RFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPAIAEQVMEEA 271

[Close]

BLAST of EX570370 vs. TrEMBL
Match: A5JVB9_BRACM (Proteasome subunit beta type OS=Brassica campestris PE=3 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 9.175e-39
Identity = 78/91 (85.71%), Postives = 82/91 (90.11%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAA 274
            RFSVGSGSPYAYGVLDSGYK+DMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P+  EQ M EAA
Sbjct:  126 RFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPNGWTKLSGDDVGELHYHYYPVPPAIAEQVMEEAA 216

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5A_ARATH (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana GN=PBE1 PE=1 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.308e-40
Identity = 79/90 (87.78%), Postives = 82/90 (91.11%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEA 271
            RFSVGSGSPYAYGVLDSGYKYDMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P+T EQ M EA
Sbjct:  182 RFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEEA 271

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_SPIOL (Proteasome subunit beta type-5 OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 6.492e-40
Identity = 75/92 (81.52%), Postives = 84/92 (91.30%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAAG 277
            RFSVGSGSPYAYGVLD+GYKYDM+VEEAS LARRAIYHAT+RDGASGGV SVY+VGP GWKK++GDDVG+LH+ Y PV P+TVEQEMVE  G
Sbjct:  180 RFSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGASGGVVSVYHVGPDGWKKVTGDDVGDLHFQYYPVVPATVEQEMVEVVG 271

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5B_ARATH (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana GN=PBE2 PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 1.107e-39
Identity = 78/91 (85.71%), Postives = 81/91 (89.01%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAA 274
            RFSVGSGSPYAYGVLDSGYK+DMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P T E  M EAA
Sbjct:  182 RFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQGWTKLSGDDVGELHYHYYPVAPITAEHVMEEAA 272

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_DICDI (Proteasome subunit beta type-5 OS=Dictyostelium discoideum GN=psmB5 PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 5.168e-21
Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTP 241
            RFS GSGS YAYGVLD+G+K+DM+ EEA  L RRA+YHAT RD  SGG  +VY+V   GWKK+S DD  +L+  Y  + P
Sbjct:  187 RFSCGSGSTYAYGVLDTGFKWDMNDEEAYELGRRAVYHATHRDAYSGGAINVYHVQKDGWKKISSDDCFKLYQKYYNIVP 266

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_PONAB (Proteasome subunit beta type-5 OS=Pongo abelii GN=PSMB5 PE=2 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 6.749e-21
Identity = 46/79 (58.23%), Postives = 58/79 (73.42%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTP 241
            FSVGSGS YAYGV+D GY YD+ VE+A  LARRAIY AT+RD  SGG  ++Y+V   GW ++S D+V +LH  Y+  TP
Sbjct:  185 FSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP 263

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_HUMAN (Proteasome subunit beta type-5 OS=Homo sapiens GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 6.749e-21
Identity = 46/79 (58.23%), Postives = 58/79 (73.42%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTP 241
            FSVGSGS YAYGV+D GY YD+ VE+A  LARRAIY AT+RD  SGG  ++Y+V   GW ++S D+V +LH  Y+  TP
Sbjct:  185 FSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP 263

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_MOUSE (Proteasome subunit beta type-5 OS=Mus musculus GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 98.9821 bits (245), Expect = 1.151e-20
Identity = 46/78 (58.97%), Postives = 58/78 (74.36%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVT 238
            FSVGSGS YAYGV+D GY YD+ VEEA  LARRAIY AT+RD  SGG  ++Y+V   GW ++S D+V +LH  Y+ V+
Sbjct:  185 FSVGSGSVYAYGVMDRGYSYDLKVEEAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHDKYSSVS 262

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_BOVIN (Proteasome subunit beta type-5 OS=Bos taurus GN=PSMB5 PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.964e-20
Identity = 47/78 (60.26%), Postives = 57/78 (73.08%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVT 238
            FSVGSGS YAYGV+D GY YD+ VEEA  LARRAIY AT+RD  SGG  S+Y+V   GW ++S D+V +LH  Y+  T
Sbjct:  185 FSVGSGSVYAYGVMDRGYSYDLEVEEAYDLARRAIYQATYRDAYSGGSVSLYHVREDGWIRVSSDNVADLHDKYSGST 262

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_RAT (Proteasome subunit beta type-5 OS=Rattus norvegicus GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 96.6709 bits (239), Expect = 5.714e-20
Identity = 45/79 (56.96%), Postives = 56/79 (70.89%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTP 241
            FSVGSGS YA+GV+D GY YD+ VEEA  LARRAIY AT+RD  SGG  ++Y+V   GW ++S D+V +LH  Y    P
Sbjct:  185 FSVGSGSVYAFGVMDRGYSYDLQVEEAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHDKYTSSIP 263

[Close]

BLAST of EX570370 vs. SwissProt
Match: PSB5_CHICK (Proteasome subunit beta type-5 (Fragment) OS=Gallus gallus GN=PSMB5 PE=2 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 9.122e-18
Identity = 43/74 (58.11%), Postives = 53/74 (71.62%), Query Frame = 2

Query:    5 FSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHY 226
            F+VGSGS YAYGVLD G + DM+ +EA  LARRAI+ A  RD  SGG  +VY+VGP GW+++S  DV  LH  Y
Sbjct:  181 FAVGSGSSYAYGVLDGGRRPDMATDEALELARRAIFQAARRDAYSGGSVTVYHVGPRGWRRVSSHDVAGLHDGY 254

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT1G13060.2 (| Symbols: PBE1 | 20S proteasome beta subunit E1 | chr1:4452641-4454663 FORWARD LENGTH=298)

HSP 1 Score: 165.236 bits (417), Expect = 1.517e-41
Identity = 79/90 (87.78%), Postives = 82/90 (91.11%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEA 271
            RFSVGSGSPYAYGVLDSGYKYDMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P+T EQ M EA
Sbjct:  206 RFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEEA 295

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT1G13060.1 (| Symbols: PBE1 | 20S proteasome beta subunit E1 | chr1:4452641-4454663 FORWARD LENGTH=274)

HSP 1 Score: 165.236 bits (417), Expect = 1.517e-41
Identity = 79/90 (87.78%), Postives = 82/90 (91.11%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEA 271
            RFSVGSGSPYAYGVLDSGYKYDMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P+T EQ M EA
Sbjct:  182 RFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEEA 271

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT3G26340.1 (| Symbols: | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr3:9650600-9652572 REVERSE LENGTH=273)

HSP 1 Score: 162.155 bits (409), Expect = 1.284e-40
Identity = 78/91 (85.71%), Postives = 81/91 (89.01%), Query Frame = 2

Query:    2 RFSVGSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKKLSGDDVGELHYHYNPVTPSTVEQEMVEAA 274
            RFSVGSGSPYAYGVLDSGYK+DMSVEEAS LARR+IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHYHY PV P T E  M EAA
Sbjct:  182 RFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQGWTKLSGDDVGELHYHYYPVAPITAEHVMEEAA 272

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT4G31300.3 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=233)

HSP 1 Score: 52.373 bits (124), Expect = 1.433e-7
Identity = 33/91 (36.26%), Postives = 46/91 (50.55%), Query Frame = 2

Query:   14 GSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKK--LSGDDVGELHYHYNPVTPSTVEQEMVEAAGP 280
            GSGS Y YG  D  +K +M+ EEA  L  +A+  A  RDGASGGV     +   G  +    GD   +L   +  + P     +++ AAGP
Sbjct:  141 GSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGD---KLQLWHEELEPQNSLLDILNAAGP 228

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT4G31300.2 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=234)

HSP 1 Score: 52.373 bits (124), Expect = 1.433e-7
Identity = 33/91 (36.26%), Postives = 46/91 (50.55%), Query Frame = 2

Query:   14 GSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKK--LSGDDVGELHYHYNPVTPSTVEQEMVEAAGP 280
            GSGS Y YG  D  +K +M+ EEA  L  +A+  A  RDGASGGV     +   G  +    GD   +L   +  + P     +++ AAGP
Sbjct:  142 GSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGD---KLQLWHEELEPQNSLLDILNAAGP 229

[Close]

BLAST of EX570370 vs. TAIR peptide
Match: AT4G31300.1 (| Symbols: PBA1 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein | chr4:15188927-15190935 FORWARD LENGTH=233)

HSP 1 Score: 52.373 bits (124), Expect = 1.433e-7
Identity = 33/91 (36.26%), Postives = 46/91 (50.55%), Query Frame = 2

Query:   14 GSGSPYAYGVLDSGYKYDMSVEEASGLARRAIYHATFRDGASGGVASVYYVGPTGWKK--LSGDDVGELHYHYNPVTPSTVEQEMVEAAGP 280
            GSGS Y YG  D  +K +M+ EEA  L  +A+  A  RDGASGGV     +   G  +    GD   +L   +  + P     +++ AAGP
Sbjct:  141 GSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVIINSEGVTRNFYPGD---KLQLWHEELEPQNSLLDILNAAGP 228

The following BLAST results are available for this feature:

BLAST of EX570370 vs. TrEMBL
BLAST of EX570370 vs. SwissProt
BLAST of EX570370 vs. TAIR peptide

BLAST of EX570370 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9ZRY9_CICAR	5.366e-47	96.77	20S proteasome beta subunit OS=Cicer arietinum PE=...	[more]
C6TLJ9_SOYBN	9.472e-44	92.39	Proteasome subunit beta type OS=Glycine max PE=2 S...	[more]
C3VIB9_CITMA	1.047e-42	90.22	20S proteasome beta subunit 5 (Fragment) OS=Citrus...	[more]
C3VIB8_CITMA	1.047e-42	90.22	Proteasome subunit beta type OS=Citrus maxima PE=2...	[more]
D7TAR8_VITVI	1.158e-41	89.13	Whole genome shotgun sequence of line PN40024, sca...	[more]
B7FKR0_MEDTR	9.802e-41	97.62	Putative uncharacterized protein (Fragment) OS=Med...	[more]
Q93WZ4_PRUPE	8.298e-40	86.81	Putative 20S proteasome beta subunit 5 (Fragment) ...	[more]
D7LR41_ARALY	8.298e-40	87.91	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7KPF3_ARALY	9.175e-39	86.67	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A5JVB9_BRACM	9.175e-39	85.71	Proteasome subunit beta type OS=Brassica campestri...	[more]

BLAST of EX570370 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PSB5A_ARATH	1.308e-40	87.78	Proteasome subunit beta type-5-A OS=Arabidopsis th...	[more]
PSB5_SPIOL	6.492e-40	81.52	Proteasome subunit beta type-5 OS=Spinacia olerace...	[more]
PSB5B_ARATH	1.107e-39	85.71	Proteasome subunit beta type-5-B OS=Arabidopsis th...	[more]
PSB5_DICDI	5.168e-21	58.75	Proteasome subunit beta type-5 OS=Dictyostelium di...	[more]
PSB5_PONAB	6.749e-21	58.23	Proteasome subunit beta type-5 OS=Pongo abelii GN=...	[more]
PSB5_HUMAN	6.749e-21	58.23	Proteasome subunit beta type-5 OS=Homo sapiens GN=...	[more]
PSB5_MOUSE	1.151e-20	58.97	Proteasome subunit beta type-5 OS=Mus musculus GN=...	[more]
PSB5_BOVIN	1.964e-20	60.26	Proteasome subunit beta type-5 OS=Bos taurus GN=PS...	[more]
PSB5_RAT	5.714e-20	56.96	Proteasome subunit beta type-5 OS=Rattus norvegicu...	[more]
PSB5_CHICK	9.122e-18	58.11	Proteasome subunit beta type-5 (Fragment) OS=Gallu...	[more]

BLAST of EX570370 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G13060.2	1.517e-41	87.78	\| Symbols: PBE1 \| 20S proteasome beta subunit E1 \|...	[more]
AT1G13060.1	1.517e-41	87.78	\| Symbols: PBE1 \| 20S proteasome beta subunit E1 \|...	[more]
AT3G26340.1	1.284e-40	85.71	\| Symbols: \| N-terminal nucleophile aminohydrolas...	[more]
AT4G31300.3	1.433e-7	36.26	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]
AT4G31300.2	1.433e-7	36.26	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]
AT4G31300.1	1.433e-7	36.26	\| Symbols: PBA1 \| N-terminal nucleophile aminohydr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000243	Peptidase T1A, proteasome beta-subunit	PRINTS	PR00141	PROTEASOME	coord: 5..16 score: 1.9e-12coord: 16..27 score: 1.9e-12coord: 41..52 score: 1.9
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 2..55 score: 8.5
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 2..79 score: 3.9
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 2..90 score: 1.9
None	No IPR available	PANTHER	PTHR11599:SF3	PROTEASOME SUBUNIT BETA TYPE 5,8	coord: 2..90 score: 1.9
None	No IPR available	PROFILE	PS51476	PROTEASOME_B_2	coord: 1..55 score: 19
None	No IPR available	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 2..77 score: 5.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570370 ID=EX570370; Name=EX570370; organism=Pisum sativum; type=EST; length=479bp
TAGATTCTCAGTTGGATCTGGTTCGCCATATGCTTATGGTGTATTGGACA
GCGGGTACAAATATGACATGTCAGTTGAGGAAGCTTCTGGACTGGCTCGA
AGAGCAATTTATCATGCAACATTTCGTGATGGAGCTAGTGGTGGAGTTGC
TAGCGTTTACTACGTGGGACCAACTGGATGGAAAAAATTGTCTGGTGATG
ATGTTGGTGAACTTCACTACCATTACAACCCGGTTACCCCTAGCACTGTG
GAGCAAGAAATGGTCGAGGCAGCTGGACCTTGATGCAGTAACTCATGGAT
ATCTTTGGAGTTTCCCATACTTGTCTGCTTACTTTGAATCAATTGCATGG
ACCTGAATTATATTCAAAACTGTCACCAGTTTTATGTATACACAAGTGCT
GCTCTATGCTCTGCTGTTGTGTCAAGTTTTCAGATTATATCAAAATTTAC
CGTGGGAATGGACAGAGCCATGTGTAATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004175	endopeptidase activity
GO:0004298	threonine-type endopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005839	proteasome core complex

Vocabulary: **Biological Process**
Term	Definition
GO:0051603	proteolysis involved in protein catabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000243	Pept_T1A_subB
IPR001353	Proteasome_sua/b

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