GH720388

EST Overview
NameGH720388
Unique NameGH720388
TypeEST
OrganismPisum sativum (pea)
Sequence length355
Libraries
Library NameType
LIBEST_024270 552Icdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of GH720388 vs. TrEMBL
Match: A9PDI1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_836929 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.198e-17
Identity = 45/45 (100.00%), Postives = 45/45 (100.00%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: C0L7D9_ANNCH (AP-2 complex subunit OS=Annona cherimola GN=AP2 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.564e-17
Identity = 44/45 (97.78%), Postives = 45/45 (100.00%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: B8LR65_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.564e-17
Identity = 44/45 (97.78%), Postives = 45/45 (100.00%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: A9TB14_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_220538 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.485e-17
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTK+L
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKDL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: A9S7M0_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_209451 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.485e-17
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTK+L
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKDL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: Q3E8F7_ARATH (Putative uncharacterized protein At5g46630.2 OS=Arabidopsis thaliana GN=At5g46630 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.944e-17
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINR YRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: O23140_ARATH (AP47/50p OS=Arabidopsis thaliana GN=At5g46630 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.944e-17
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINR YRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: D7MRC0_ARALY (Clathrin adaptor complexes medium subunit family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_916763 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.944e-17
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINR YRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: Q6ZGX8_ORYSJ (Os02g0690700 protein OS=Oryza sativa subsp. japonica GN=OJ1743_B12.40 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.763e-17
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFR HIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRMHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TrEMBL
Match: D7TU01_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_12.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00013199001 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.763e-17
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINRLYRDDVGGNMVDAFR HIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRLYRDDVGGNMVDAFRMHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TAIR peptide
Match: AT5G46630.2 (| Symbols: | Clathrin adaptor complexes medium subunit family protein | chr5:18920580-18923060 FORWARD LENGTH=441)

HSP 1 Score: 90.5077 bits (223), Expect = 2.232e-19
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINR YRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKEL 45
[Close]
BLAST of GH720388 vs. TAIR peptide
Match: AT5G46630.1 (| Symbols: | Clathrin adaptor complexes medium subunit family protein | chr5:18920580-18923252 FORWARD LENGTH=438)

HSP 1 Score: 90.5077 bits (223), Expect = 2.232e-19
Identity = 43/45 (95.56%), Postives = 44/45 (97.78%), Query Frame = -2 
Query:   28 MPVAASAVYFLNLRGDVLINRLYRDDVGGNMVDAFRTHIMQTKEL 162
            MPVAASA+YFLNLRGDVLINR YRDDVGGNMVDAFRTHIMQTKEL
Sbjct:    1 MPVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKEL 45
The following BLAST results are available for this feature:
BLAST of GH720388 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
A9PDI1_POPTR1.198e-17100.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
C0L7D9_ANNCH1.564e-1797.78AP-2 complex subunit OS=Annona cherimola GN=AP2 PE... [more]
B8LR65_PICSI1.564e-1797.78Putative uncharacterized protein OS=Picea sitchens... [more]
A9TB14_PHYPA3.485e-1795.56Predicted protein OS=Physcomitrella patens subsp. ... [more]
A9S7M0_PHYPA3.485e-1795.56Predicted protein OS=Physcomitrella patens subsp. ... [more]
Q3E8F7_ARATH5.944e-1795.56Putative uncharacterized protein At5g46630.2 OS=Ar... [more]
O23140_ARATH5.944e-1795.56AP47/50p OS=Arabidopsis thaliana GN=At5g46630 PE=2... [more]
D7MRC0_ARALY5.944e-1795.56Clathrin adaptor complexes medium subunit family p... [more]
Q6ZGX8_ORYSJ7.763e-1795.56Os02g0690700 protein OS=Oryza sativa subsp. japoni... [more]
D7TU01_VITVI7.763e-1795.56Whole genome shotgun sequence of line PN40024, sca... [more]
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BLAST of GH720388 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G46630.22.232e-1995.56| Symbols: | Clathrin adaptor complexes medium su... [more]
AT5G46630.12.232e-1995.56| Symbols: | Clathrin adaptor complexes medium su... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015629Clathrin coat associated protein AP-50PANTHERPTHR11998:SF12CLATHRIN COAT ASSOCIATED PROTEIN AP-50coord: 11..54
score: 5.5
NoneNo IPR availableGENE3D3.30.450.60no descriptioncoord: 10..49
score: 3.6
NoneNo IPR availablePANTHERPTHR11998CLATHRIN COAT ASSEMBLY PROTEINcoord: 11..54
score: 5.5

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH720388 ID=GH720388; Name=GH720388; organism=Pisum sativum; type=EST; length=355bp
CGATTAGCGTGGTCGCGGCCGAGGTACAAGTTCTTTTGTTTGCATTATAT
GCGTCCTAAAAGCATCAACCATATTCCCCCCAACATCGTCGCGATAGAGA
CGATTGATGAGAACGTCTCCGCGGAGGTTCAAGAAGTAGACAGCGGAAGC
AGCCACCGGCATCTCCGATCCAGTGTATCAACTTCCAATTTCACCGAAGA
AGAGCAGGATCAATCAGAAAGGTGGTTACGCCGTAGATCTGTGGGTTTTT
GAGGAAGAAAAACACGGAAACGTCGTCGACGGTGAGATGAGAAAAGAAGC
GCTTAACAAAATGAGAAATATTCAGACTCCGTTATGAGACCTGCCCGGGC
GGCCG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: Biological Process
TermDefinition
GO:0006886intracellular protein transport
GO:0016192vesicle-mediated transport
Vocabulary: Cellular Component
TermDefinition
GO:0030131clathrin adaptor complex
GO:0030132clathrin coat of coated pit
Vocabulary: INTERPRO
TermDefinition
IPR015629Clathrin coat associated protein AP-50