AM162010

EST Overview


Name	AM162010
Unique Name	AM162010
Type	EST
Organism	Pisum sativum (pea)
Sequence length	326

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162010 vs. TrEMBL
Match: B9T0E4_RICCO (Ubiquitin conjugating enzyme, putative OS=Ricinus communis GN=RCOM_0507780 PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 7.761e-38
Identity = 71/105 (67.62%), Postives = 86/105 (81.90%), Query Frame = -1

Query:    3 TISDEAREEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 317
            T+     ++IL K+QNFK+FDTV+D SDH++    SS  Q  KNW K+IQEEW++LE DLPD IFVR+YESR+DLLRAVIIGAEGTPYHDG+FFFDV FPSG+PN
Sbjct:  346 TLKHVNEDDILSKYQNFKRFDTVEDHSDHHYASKGSSVKQPPKNWAKKIQEEWRILENDLPDGIFVRIYESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSGYPN 450

[Close]

BLAST of AM162010 vs. TrEMBL
Match: B9HZU1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1094251 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 1.239e-35
Identity = 69/104 (66.35%), Postives = 85/104 (81.73%), Query Frame = -1

Query:    6 TISDEAREEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFP 317
            T+ +  +++IL KFQ FK+FDTV+D SDH++    S   Q  K W KRIQEEW++LE DLPDSIFVRVYE+R+DLLRAVIIGAEGTPYHDG+FFFDV FP+G+P
Sbjct:  180 TLKNVNKDDILRKFQLFKRFDTVEDHSDHHYTSKGSLMKQPPKTWAKRIQEEWRILENDLPDSIFVRVYETRMDLLRAVIIGAEGTPYHDGLFFFDVFFPAGYP 283

[Close]

BLAST of AM162010 vs. TrEMBL
Match: B9N1H3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_263467 PE=3 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 4.407e-33
Identity = 67/98 (68.37%), Postives = 80/98 (81.63%), Query Frame = -1

Query:    6 KFQNFKQFDTVDDTSDHYFVRTNS------STTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFP 281
            KFQ FKQFDTV+D SDH++  ++S      S  Q  K W KRIQ+EW++LE DLPDSIFVRVYE+R+DLLRAVIIGAEGTPYHDG+FFFDV FP+G+P
Sbjct:    1 KFQQFKQFDTVEDHSDHHYTNSSSMKQVTSSFWQSPKTWAKRIQDEWRILENDLPDSIFVRVYETRMDLLRAVIIGAEGTPYHDGLFFFDVFFPAGYP 98

[Close]

BLAST of AM162010 vs. TrEMBL
Match: D7L425_ARALY (Ubiquitin-conjugating enzyme 23 OS=Arabidopsis lyrata subsp. lyrata GN=UBC25 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 9.818e-33
Identity = 63/98 (64.29%), Postives = 79/98 (80.61%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            E+ L  +  FK+FD V+D SDH++    +S+ QHSK W KRIQ+EWK+LEKDLPD IFVR YESR+DLLRAVIIGA+GTPYHDG+FFFD+ FP  +P+
Sbjct:  292 EDFLGLYLFFKRFDIVEDFSDHHYASKGNSSKQHSKEWAKRIQDEWKILEKDLPDMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPS 389

[Close]

BLAST of AM162010 vs. TrEMBL
Match: C5YVG0_SORBI (Putative uncharacterized protein Sb09g028100 OS=Sorghum bicolor GN=Sb09g028100 PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.418e-31
Identity = 64/102 (62.75%), Postives = 79/102 (77.45%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQ----HSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            ++I EK++ FK+FDTVDD SDHY+ +      Q     SK+WVKRIQ EWK+LEKDLPD+IFVR YE R+DLLRAVI+G  GTPYHDG+FFFD+ FP  +PN
Sbjct:  202 DKIEEKYKAFKRFDTVDDHSDHYYSKPEKRRVQVVKKPSKDWVKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVIMGPAGTPYHDGLFFFDIYFPPQYPN 303

[Close]

BLAST of AM162010 vs. TrEMBL
Match: C4JA43_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.418e-31
Identity = 64/102 (62.75%), Postives = 80/102 (78.43%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQH----SKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            ++I EK++ FK+FDTVDD SDHY+ +  S   Q     SK+WVKRIQ EWK+LEKDLP++IFVR YE R+DLLRAVI+G  GTPYHDG+FFFD+ FP  +PN
Sbjct:  202 DKIEEKYKAFKRFDTVDDHSDHYYSKPESRKVQAVKKPSKDWVKRIQHEWKVLEKDLPETIFVRAYEDRMDLLRAVIMGPAGTPYHDGLFFFDIYFPPQYPN 303

[Close]

BLAST of AM162010 vs. TrEMBL
Match: B9RVZ2_RICCO (Ubiquitin conjugating enzyme, putative OS=Ricinus communis GN=RCOM_1174270 PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.418e-31
Identity = 65/104 (62.50%), Postives = 79/104 (75.96%), Query Frame = -1

Query:    3 DEAREEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHS--KNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 308
            D  R+ +L   +NFKQFDTV+D SDH++ R  SS  Q    + W KRIQEEWK+LEKDLPD+I VRVYESR++LLRAVI+G  GTPYHDG+F FD  FPS +PN
Sbjct:  220 DNKRKGVLHILKNFKQFDTVEDFSDHHYCRMESSDKQRPDPRGWAKRIQEEWKILEKDLPDTISVRVYESRMELLRAVIVGPAGTPYHDGLFVFDCLFPSTYPN 323

[Close]

BLAST of AM162010 vs. TrEMBL
Match: D7KKD2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_892327 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.125e-31
Identity = 61/98 (62.24%), Postives = 81/98 (82.65%), Query Frame = -1

Query:    3 EILEKF-QNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 293
            +I E+F ++FK+FDTV+D SDH++     S+ QHSKNWVK++Q +WK+LE DLP++I VR  ESR+DLLRAVIIGAEGTPYHDG+FFFD+ FP  +P+
Sbjct:  237 KIKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPS 334

[Close]

BLAST of AM162010 vs. TrEMBL
Match: C4J984_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 5.387e-31
Identity = 63/102 (61.76%), Postives = 79/102 (77.45%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQ----HSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            ++I EK++ FK+FDTVDD SDHY+ +  S   Q     SK+WVKRIQ EWK+LE+DLPD+IFVR YE R+DLLR VI+G  GTPYHDG+FFFD+ FP  +PN
Sbjct:  195 DKIEEKYKAFKRFDTVDDHSDHYYSKPTSRKVQVVKKPSKDWVKRIQHEWKVLERDLPDTIFVRAYEDRMDLLRGVIMGPAGTPYHDGLFFFDIYFPPRYPN 296

[Close]

BLAST of AM162010 vs. TrEMBL
Match: Q6I602_ORYSJ (Os05g0557600 protein OS=Oryza sativa subsp. japonica GN=OJ1214_E03.17 PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.560e-30
Identity = 63/102 (61.76%), Postives = 76/102 (74.51%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQ----HSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            ++I EK+  FKQFDTVDD SDHY+ + +    Q     SK W KRIQ EWK+LEKDLPD+IFVR YE R+DLLRAVI G  GTPYHDG+FFFD+ FP  +P+
Sbjct:  204 DKIDEKYSAFKQFDTVDDHSDHYYSKPDLRKVQVVKKPSKEWAKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVITGPAGTPYHDGLFFFDIYFPPHYPS 305

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBC26_ARATH (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana GN=UBC26 PE=2 SV=2)

HSP 1 Score: 139.043 bits (349), Expect = 7.122e-33
Identity = 62/103 (60.19%), Postives = 81/103 (78.64%), Query Frame = -1

Query:    3 SDEAREEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 311
            S  AR+   E  ++FK+FDTV+D SDH++     S+ QHSKNWVK++Q +WK+LE DLP++I VR  ESR+DLLRAVIIGAEGTPYHDG+FFFD+ FP  +P+
Sbjct:  230 SSNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPS 332

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBC25_ARATH (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana GN=UBC25 PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 7.122e-33
Identity = 60/98 (61.22%), Postives = 80/98 (81.63%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            E+ L  +  FK+FD V+D SDH++    +++ QHSK+W KRIQ+EW++LEKDLP+ IFVR YESR+DLLRAVIIGA+GTPYHDG+FFFD+ FP  +P+
Sbjct:  296 EDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPS 393

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBC24_ARATH (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana GN=UBC24 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 4.185e-25
Identity = 51/101 (50.50%), Postives = 75/101 (74.26%), Query Frame = -1

Query:    3 ILEKFQN---FKQFDTVDDTSDHYFVRTNSSTTQH--SKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 290
            +LE  +N   F+QFD V+D SDH+F+ ++    Q   +K+WVK++Q+EW  LE +LP++I+VRV E R+DLLRA ++GA GTPYHDG+FFFD+  P  +P+
Sbjct:  623 LLENSRNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPH 723

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBC23_ARATH (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana GN=UBC23 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 3.917e-23
Identity = 45/90 (50.00%), Postives = 65/90 (72.22%), Query Frame = -1

Query:    3 NFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 272
            +F++FD   D  DH+F+  +   T+  + W K++ ++WK+L+ +LPD IFVR YE R+DLLRAVI+GA GTPY DG+FFFD   PS +P+
Sbjct:  823 SFRRFDISQDPLDHHFLGVDGQKTKE-RQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPS 911

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBE2O_MOUSE (Ubiquitin-conjugating enzyme E2 O OS=Mus musculus GN=Ube2o PE=1 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 4.204e-9
Identity = 28/78 (35.90%), Postives = 45/78 (57.69%), Query Frame = -1

Query:    6 SDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFP 239
            S+H F +      + +K +   +++E  LL   LPD I V+ +E R+DL  A+I G   TPY DG++ FD+  P+ +P
Sbjct:  934 SNHSFKKIEFQPPE-AKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYP 1010

[Close]

BLAST of AM162010 vs. SwissProt
Match: BIRC6_HUMAN (Baculoviral IAP repeat-containing protein 6 OS=Homo sapiens GN=BIRC6 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.366e-9
Identity = 36/121 (29.75%), Postives = 60/121 (49.59%), Query Frame = -1

Query:    3 TVVTISDEAREEILEKFQNFKQFDTVDDTSD---------HYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLP----DSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 326
            +V+   +E    +++K Q F  F+ V +  D         HY  +  ++   +S    +R+ +E   L   LP     S+FVR  E R+D+++ +I G   TPY +G F FDV FP  +P+
Sbjct: 4491 SVLKSLEEKYVAVMKKLQ-FDTFEMVSEDEDGKLGFKVNYHYMSQVKNANDANSAARARRLAQEAVTLSTSLPLSSSSSVFVRCDEERLDIMKVLITGPADTPYANGCFEFDVYFPQDYPS 4610

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBE2O_HUMAN (Ubiquitin-conjugating enzyme E2 O OS=Homo sapiens GN=UBE2O PE=1 SV=3)

HSP 1 Score: 58.5362 bits (140), Expect = 1.223e-8
Identity = 27/78 (34.62%), Postives = 45/78 (57.69%), Query Frame = -1

Query:    6 SDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFP 239
            S+H F +      + +K +   +++E  LL   LP+ I V+ +E R+DL  A+I G   TPY DG++ FD+  P+ +P
Sbjct:  937 SNHSFKKIEFQPPE-AKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYP 1013

[Close]

BLAST of AM162010 vs. SwissProt
Match: UBC17_CAEEL (Probable ubiquitin-conjugating enzyme protein 17 OS=Caenorhabditis elegans GN=ubc-17 PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 1.766e-7
Identity = 26/68 (38.24%), Postives = 43/68 (63.24%), Query Frame = -1

Query:    6 HSKNWVKRIQEEWKLLEKDLP----DSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFP 197
            H ++  KRI +E   +   LP    +SI+V   E R+D+++ +I G + TPY +G+F FD+ FP+G+P
Sbjct:  406 HLRDRTKRIAKELASIANALPLNASNSIYVCYDEGRVDIIKVLISGPDDTPYANGLFEFDIFFPTGYP 473

[Close]

BLAST of AM162010 vs. TAIR peptide
Match: AT3G15355.1 (| Symbols: UBC25, PFU1 | ubiquitin-conjugating enzyme 25 | chr3:5183858-5186669 FORWARD LENGTH=609)

HSP 1 Score: 139.043 bits (349), Expect = 5.641e-34
Identity = 60/98 (61.22%), Postives = 80/98 (81.63%), Query Frame = -1

Query:    3 EEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 296
            E+ L  +  FK+FD V+D SDH++    +++ QHSK+W KRIQ+EW++LEKDLP+ IFVR YESR+DLLRAVIIGA+GTPYHDG+FFFD+ FP  +P+
Sbjct:  296 EDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPS 393

[Close]

BLAST of AM162010 vs. TAIR peptide
Match: AT1G53025.1 (| Symbols: | Ubiquitin-conjugating enzyme family protein | chr1:19757072-19759474 REVERSE LENGTH=543)

HSP 1 Score: 139.043 bits (349), Expect = 5.641e-34
Identity = 62/103 (60.19%), Postives = 81/103 (78.64%), Query Frame = -1

Query:    3 SDEAREEILEKFQNFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 311
            S  AR+   E  ++FK+FDTV+D SDH++     S+ QHSKNWVK++Q +WK+LE DLP++I VR  ESR+DLLRAVIIGAEGTPYHDG+FFFD+ FP  +P+
Sbjct:  230 SSNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPS 332

[Close]

BLAST of AM162010 vs. TAIR peptide
Match: AT1G53023.1 (| Symbols: | Ubiquitin-conjugating enzyme family protein | chr1:19754214-19755843 REVERSE LENGTH=316)

HSP 1 Score: 125.561 bits (314), Expect = 6.454e-30
Identity = 63/118 (53.39%), Postives = 84/118 (71.19%), Query Frame = -1

Query:    3 VVTISDEAR-EEILEKF-QNFKQFDTVDDTSDHYFVRTNSSTTQ---------HSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 323
            +V I D +   ++ E+F ++FK+FDTV+D SDH++    S               KNWVK IQ+EWK+L+K+LP++IFVR  ESRIDLLRAVIIGAEGTPYHDG+FFFD+ FP  +P+
Sbjct:    1 MVEIKDSSNVRKLKEEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPS 118

[Close]

BLAST of AM162010 vs. TAIR peptide
Match: AT2G33770.1 (| Symbols: UBC24, ATUBC24, PHO2 | phosphate 2 | chr2:14277785-14281482 REVERSE LENGTH=907)

HSP 1 Score: 113.235 bits (282), Expect = 3.315e-26
Identity = 51/101 (50.50%), Postives = 75/101 (74.26%), Query Frame = -1

Query:    3 ILEKFQN---FKQFDTVDDTSDHYFVRTNSSTTQH--SKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 290
            +LE  +N   F+QFD V+D SDH+F+ ++    Q   +K+WVK++Q+EW  LE +LP++I+VRV E R+DLLRA ++GA GTPYHDG+FFFD+  P  +P+
Sbjct:  623 LLENSRNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPH 723

[Close]

BLAST of AM162010 vs. TAIR peptide
Match: AT2G16920.1 (| Symbols: UBC23, PFU2 | ubiquitin-conjugating enzyme 23 | chr2:7334559-7338827 REVERSE LENGTH=1102)

HSP 1 Score: 106.686 bits (265), Expect = 3.103e-24
Identity = 45/90 (50.00%), Postives = 65/90 (72.22%), Query Frame = -1

Query:    3 NFKQFDTVDDTSDHYFVRTNSSTTQHSKNWVKRIQEEWKLLEKDLPDSIFVRVYESRIDLLRAVIIGAEGTPYHDGIFFFDVSFPSGFPN 272
            +F++FD   D  DH+F+  +   T+  + W K++ ++WK+L+ +LPD IFVR YE R+DLLRAVI+GA GTPY DG+FFFD   PS +P+
Sbjct:  823 SFRRFDISQDPLDHHFLGVDGQKTKE-RQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPS 911

The following BLAST results are available for this feature:

BLAST of AM162010 vs. TrEMBL
BLAST of AM162010 vs. SwissProt
BLAST of AM162010 vs. TAIR peptide

BLAST of AM162010 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T0E4_RICCO	7.761e-38	67.62	Ubiquitin conjugating enzyme, putative OS=Ricinus ...	[more]
B9HZU1_POPTR	1.239e-35	66.35	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9N1H3_POPTR	4.407e-33	68.37	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7L425_ARALY	9.818e-33	64.29	Ubiquitin-conjugating enzyme 23 OS=Arabidopsis lyr...	[more]
C5YVG0_SORBI	2.418e-31	62.75	Putative uncharacterized protein Sb09g028100 OS=So...	[more]
C4JA43_MAIZE	2.418e-31	62.75	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B9RVZ2_RICCO	2.418e-31	62.50	Ubiquitin conjugating enzyme, putative OS=Ricinus ...	[more]
D7KKD2_ARALY	4.125e-31	62.24	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C4J984_MAIZE	5.387e-31	61.76	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q6I602_ORYSJ	4.560e-30	61.76	Os05g0557600 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of AM162010 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
UBC26_ARATH	7.122e-33	60.19	Probable ubiquitin-conjugating enzyme E2 26 OS=Ara...	[more]
UBC25_ARATH	7.122e-33	61.22	Probable ubiquitin-conjugating enzyme E2 25 OS=Ara...	[more]
UBC24_ARATH	4.185e-25	50.50	Probable ubiquitin-conjugating enzyme E2 24 OS=Ara...	[more]
UBC23_ARATH	3.917e-23	50.00	Probable ubiquitin-conjugating enzyme E2 23 OS=Ara...	[more]
UBE2O_MOUSE	4.204e-9	35.90	Ubiquitin-conjugating enzyme E2 O OS=Mus musculus ...	[more]
BIRC6_HUMAN	9.366e-9	29.75	Baculoviral IAP repeat-containing protein 6 OS=Hom...	[more]
UBE2O_HUMAN	1.223e-8	34.62	Ubiquitin-conjugating enzyme E2 O OS=Homo sapiens ...	[more]
UBC17_CAEEL	1.766e-7	38.24	Probable ubiquitin-conjugating enzyme protein 17 O...	[more]

BLAST of AM162010 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G15355.1	5.641e-34	61.22	\| Symbols: UBC25, PFU1 \| ubiquitin-conjugating enz...	[more]
AT1G53025.1	5.641e-34	60.19	\| Symbols: \| Ubiquitin-conjugating enzyme family ...	[more]
AT1G53023.1	6.454e-30	53.39	\| Symbols: \| Ubiquitin-conjugating enzyme family ...	[more]
AT2G33770.1	3.315e-26	50.50	\| Symbols: UBC24, ATUBC24, PHO2 \| phosphate 2 \| ch...	[more]
AT2G16920.1	3.103e-24	50.00	\| Symbols: UBC23, PFU2 \| ubiquitin-conjugating enz...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 16..31 score
None	No IPR available	PANTHER	PTHR11621	UBIQUITIN-CONJUGATING ENZYME E2	coord: 11..109 score: 3.4
None	No IPR available	PANTHER	PTHR11621:SF12	UBIQUITIN-CONJUGATING ENZYME-RELATED	coord: 11..109 score: 3.4
IPR000608	Ubiquitin-conjugating enzyme, E2	PFAM	PF00179	UQ_con	coord: 51..108 score: 2.4
IPR000608	Ubiquitin-conjugating enzyme, E2	PROFILE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 50..109 score: 10
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	no description	coord: 9..108 score: 1.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 42..109 score: 3.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162010 ID=AM162010; Name=AM162010; organism=Pisum sativum; type=EST; length=326bp
ACATTGGGAAAGCCACTTGGAAAGGAAACATCAAAAAAGAAAATGCCATC
ATGATAAGGAGTTCCCTCTGCTCCAATAATTACAGCCCTCAACAGATCTA
TTCTTGATTCATAAACTCTGACAAATATTGAATCTGGTAAATCCTTCTCC
AATAACTTCCATTCTTCCTGAATTCTTTTAACCCAATTCTTTGAATGCTG
TGTCGTGGAAGAATTGGTGCGAACAAAGTAATGGTCTGAAGTGTCGTCAA
CAGTGTCAAATTGCTTAAAGTTTTGAAATTTCTCAAGAATTTCTTCTCTG
GCTTCATCAGAGATGGTCACAACAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016881	acid-amino acid ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0043687	post-translational protein modification

Vocabulary: **INTERPRO**
Term	Definition
IPR000608	UBQ-conjugat_E2
IPR016135	UBQ-conjugating_enzyme/RWD

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