FG533682

EST Overview


Name	FG533682
Unique Name	FG533682
Type	EST
Organism	Pisum sativum (pea)
Sequence length	688

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG533682 vs. TrEMBL
Match: B9T410_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0485220 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.756e-36
Identity = 57/128 (44.53%), Postives = 80/128 (62.50%), Query Frame = 1

Query:    1 IAWAKEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQ-------TETEK---------LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            IAWAKEKFQS S + +   K D + +  +   S++  E   +G G+ + P +++        ETE+         LDE+IR+WS GKET+IRLLLSTLHHILW  SGW+++ L +L  +S VK+AYQK
Sbjct:  313 IAWAKEKFQSQSFDRQQ--KKDHSNELEETPNSNEFLEQQMDGHGRTQSPTEEELKNLRSEAETEQSEKDMGMELLDEDIRLWSAGKETNIRLLLSTLHHILWPNSGWFSIPLTSLIESSHVKKAYQK 438

HSP 2 Score: 74.3294 bits (181), Expect = 3.756e-36
Identity = 35/48 (72.92%), Postives = 40/48 (83.33%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +ARLCLH DKLQQ+GAT   KYV E+AFSILQDAWAAFIS+DV
Sbjct:  433 KKAYQKARLCLHPDKLQQRGATHPQKYVAEKAFSILQDAWAAFISQDV 480

[Close]

BLAST of FG533682 vs. TrEMBL
Match: D7TKC2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021545001 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.284e-35
Identity = 55/127 (43.31%), Postives = 75/127 (59.06%), Query Frame = 1

Query:    1 IAWAKEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGI---GKVEPPKKQQTETEK------------LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            IAWAKEKFQ+ S  +E  ++    E  V+ +   ++ E+    +   G ++ P K +  TE             LDE++R+WS GKE +IRLLLSTLHHILW  SGWY + L +L   SQVK+AYQK
Sbjct:  315 IAWAKEKFQTHSSEKEWSIRPQETEHFVEMEDRPNSREFRVPQMDEHGMMQSPVKDKWATEDKQQMEKDMEIELLDEDVRLWSSGKEANIRLLLSTLHHILWPNSGWYAIPLTSLIETSQVKKAYQK 441

HSP 2 Score: 71.633 bits (174), Expect = 5.284e-35
Identity = 34/48 (70.83%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +ARLCLH DKLQQ+G T   KYV E+AFSILQDAWAAFIS+DV
Sbjct:  436 KKAYQKARLCLHPDKLQQRGVTPPQKYVAEKAFSILQDAWAAFISQDV 483

[Close]

BLAST of FG533682 vs. TrEMBL
Match: B9HZP4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_771319 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 9.902e-31
Identity = 40/57 (70.18%), Postives = 48/57 (84.21%), Query Frame = 1

Query:  166 ETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            E E LDE+IR+WS GKET+IRLLLSTLHHILW  SGWY ++L +L  +SQVK+AYQK
Sbjct:    2 EMELLDEDIRLWSAGKETNIRLLLSTLHHILWPNSGWYAIALTSLIESSQVKKAYQK 58

HSP 2 Score: 71.2478 bits (173), Expect = 9.902e-31
Identity = 32/48 (66.67%), Postives = 39/48 (81.25%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +ARLCLH DKLQQ+G T   KYV E+AFSILQDAWA+FIS+D+
Sbjct:   53 KKAYQKARLCLHPDKLQQRGGTLSQKYVAEKAFSILQDAWASFISQDL 100

[Close]

BLAST of FG533682 vs. TrEMBL
Match: A5AZP9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032618 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.210e-29
Identity = 55/127 (43.31%), Postives = 75/127 (59.06%), Query Frame = 1

Query:    1 IAWAKEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGI---GKVEPPKKQQTETEK------------LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            IAWAKEKFQ+ S  +E  ++    E  V+ +   ++ E+    +   G ++ P K +  TE             LDE++R+WS GKE +IRLLLSTLHHILW  SGWY + L +L   SQVK+AYQK
Sbjct:  315 IAWAKEKFQTHSSEKEWSIRPQETEHFVEMEDRPNSREFRVPQMDEHGMMQSPVKDKWATEDKQQMEKDMEIELLDEDVRLWSSGKEANIRLLLSTLHHILWPNSGWYAIPLTSLIETSQVKKAYQK 441

HSP 2 Score: 52.7582 bits (125), Expect = 2.210e-29
Identity = 25/38 (65.79%), Postives = 29/38 (76.32%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQD 431
            +K   +ARLCLH DKLQQ+G T   KYV E+AFSILQD
Sbjct:  436 KKAYQKARLCLHPDKLQQRGVTPPQKYVAEKAFSILQD 473

[Close]

BLAST of FG533682 vs. TrEMBL
Match: B9H411_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_648413 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.735e-26
Identity = 35/55 (63.64%), Postives = 45/55 (81.82%), Query Frame = 1

Query:  172 EKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            E LDE+IR+WS G+ET+IRLLLSTLH ILW +SGWY + L +L  +SQVK+ +QK
Sbjct:    2 ELLDEDIRLWSAGRETNIRLLLSTLHPILWPDSGWYAIPLTSLVESSQVKKVHQK 56

HSP 2 Score: 66.6254 bits (161), Expect = 1.735e-26
Identity = 30/42 (71.43%), Postives = 35/42 (83.33%), Query Frame = 3

Query:  333 EARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISED 458
            +ARLCLH DKLQQ+GAT   KYV E+ FSIL DAWAAF+S+D
Sbjct:   56 KARLCLHPDKLQQRGATLPQKYVAEKTFSILLDAWAAFVSQD 97

[Close]

BLAST of FG533682 vs. TrEMBL
Match: D7KES8_ARALY (Heat shock protein binding protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473251 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 6.056e-26
Identity = 47/112 (41.96%), Postives = 64/112 (57.14%), Query Frame = 1

Query:    1 IAWAKEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            IAWAKE+ Q     +       + E  +  + S +            EP  +++ E E  DE IR+W  GKET+IRLLLSTLHH+LW  S W+++ L NL+  SQVK+AYQK
Sbjct:  317 IAWAKERSQRPEAKQ-------TEEDVIDSRRSEE------------EPKSEEEMEMEMKDEEIRIWLTGKETNIRLLLSTLHHVLWSNSNWHSIPLANLRDGSQVKKAYQK 409

HSP 2 Score: 51.9878 bits (123), Expect = 6.056e-26
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATF-LHKYVGEQAFSILQDAWAAFISED 458
            +K   +ARLCLH DKLQQ+G T  + K V  + F+ILQ+AWA +++ +
Sbjct:  404 KKAYQKARLCLHPDKLQQRGGTSPIQKSVASRVFAILQEAWAVYVTNE 451

[Close]

BLAST of FG533682 vs. TrEMBL
Match: Q9C752_ARATH (Putative uncharacterized protein At1g30280 OS=Arabidopsis thaliana GN=At1g30280 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.494e-25
Identity = 44/112 (39.29%), Postives = 67/112 (59.82%), Query Frame = 1

Query:   13 KEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGI----GKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            +E+   G  N +S   +++     +R    +A +  ++ I     + EP  +++ E E  DE IR+W  GKET+IRLLLSTLHH+LW  S W ++ L NL+  SQVK+AYQ+
Sbjct:  298 REEGGGGGGNSDSNDMDEAIAWAKERSQRPEAKQTEEDVIDSRRSEEEPKSEEEMEMEMKDEEIRIWLTGKETNIRLLLSTLHHVLWSNSNWDSIPLANLRDGSQVKKAYQR 409

HSP 2 Score: 51.6026 bits (122), Expect = 6.494e-25
Identity = 23/48 (47.92%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATF-LHKYVGEQAFSILQDAWAAFISED 458
            +K    ARLCLH DKLQQ+G T  + K V  + F+ILQ+AWA +++ +
Sbjct:  404 KKAYQRARLCLHPDKLQQRGGTSPIQKSVASRVFAILQEAWAVYVTNE 451

[Close]

BLAST of FG533682 vs. TrEMBL
Match: C5Y7H1_SORBI (Putative uncharacterized protein Sb05g026150 OS=Sorghum bicolor GN=Sb05g026150 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 7.321e-19
Identity = 35/102 (34.31%), Postives = 56/102 (54.90%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +       ++    + LD  I+ WS GKE ++R LLSTL +ILW E GW  VSL +L   + VK+ Y+K
Sbjct:  766 GESEERRRARLERHQRTRERAAKALA-EKNERDMQVQREQAERDRIGDSLDFEIKRWSAGKEGNLRALLSTLQYILWPECGWQAVSLTDLITGAAVKKQYRK 866

HSP 2 Score: 55.8398 bits (133), Expect = 7.321e-19
Identity = 24/48 (50.00%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGAT   KY+ E+ F IL++AW  F SE++
Sbjct:  861 KKQYRKATLCIHPDKVQQKGATLQQKYIAEKVFDILKEAWNKFNSEEL 908

[Close]

BLAST of FG533682 vs. TrEMBL
Match: C0PDH9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 9.523e-19
Identity = 35/102 (34.31%), Postives = 56/102 (54.90%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +       ++    + LD  I+ WS GKE ++R LLSTL +ILW E GW  VSL +L   + VK+ Y+K
Sbjct:  755 GESEERRRARLERHQRTRERAAKALA-EKNERDMQVQREQAERDRIGDTLDFEIKRWSAGKEGNLRALLSTLQYILWPECGWQAVSLTDLITGAAVKKQYRK 855

HSP 2 Score: 55.8398 bits (133), Expect = 9.523e-19
Identity = 24/48 (50.00%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGAT   KY+ E+ F IL++AW  F SE++
Sbjct:  850 KKQYRKATLCIHPDKVQQKGATLQQKYIAEKVFDILKEAWNKFNSEEL 897

[Close]

BLAST of FG533682 vs. TrEMBL
Match: B8A0P4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 9.523e-19
Identity = 35/102 (34.31%), Postives = 56/102 (54.90%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +       ++    + LD  I+ WS GKE ++R LLSTL +ILW E GW  VSL +L   + VK+ Y+K
Sbjct:  755 GESEERRRARLERHQRTRERAAKALA-EKNERDMQVQREQAERDRIGDTLDFEIKRWSAGKEGNLRALLSTLQYILWPECGWQAVSLTDLITGAAVKKQYRK 855

HSP 2 Score: 55.8398 bits (133), Expect = 9.523e-19
Identity = 24/48 (50.00%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGAT   KY+ E+ F IL++AW  F SE++
Sbjct:  850 KKQYRKATLCIHPDKVQQKGATLQQKYIAEKVFDILKEAWNKFNSEEL 897

[Close]

BLAST of FG533682 vs. SwissProt
Match: AUXI2_ARATH (Auxilin-related protein 2 OS=Arabidopsis thaliana GN=At4g12770 PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 8.840e-19
Identity = 36/106 (33.96%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK----LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +      +++Q E ++    LD  IR W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  748 GETEERRRARLERHQRTQERAAKALA-EKNERDL----QVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITGASVKKVYRK 848

HSP 2 Score: 52.373 bits (124), Expect = 8.840e-19
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  843 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 890

[Close]

BLAST of FG533682 vs. SwissProt
Match: AUXI1_ARATH (Auxilin-related protein 1 OS=Arabidopsis thaliana GN=At4g12780 PE=1 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 3.283e-18
Identity = 35/106 (33.02%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK----LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +      +++Q E ++    LD  I+ W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  761 GETEERRRARLERHQRTQERAAKALA-EKNERDL----QVQREQVEKDRIGVTLDVEIKRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITAASVKKVYRK 861

HSP 2 Score: 52.373 bits (124), Expect = 3.283e-18
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  856 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 903

[Close]

BLAST of FG533682 vs. SwissProt
Match: UCP7_SCHPO (UBA domain-containing protein 7 OS=Schizosaccharomyces pombe GN=ucp7 PE=2 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 5.295e-8
Identity = 27/78 (34.62%), Postives = 40/78 (51.28%), Query Frame = 1

Query:  136 KVEPPKKQQTETEKLDEN-----------IRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            +++  +  Q + E+LDE            +  W  GKE+++R LL++L  ILW E  W  VSL  L    +VK AY K
Sbjct:  578 RLQHVRMAQQKAEQLDEERSRLREPVQQIVNKWKEGKESNLRALLASLDTILWPECRWQKVSLSELVLPKKVKIAYMK 655

HSP 2 Score: 31.187 bits (69), Expect = 5.295e-8
Identity = 15/33 (45.45%), Postives = 20/33 (60.61%), Query Frame = 3

Query:  348 LHTDKLQQKGATFLHKYVGEQAFSILQDAWAAF 446
            +H DKL Q+  +  H+ + E AFSIL  AW  F
Sbjct:  660 VHPDKLPQQ-TSVEHQLIAESAFSILNHAWELF 691

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT1G30280.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr1:10662851-10664570 REVERSE LENGTH=455)

HSP 1 Score: 87.4261 bits (215), Expect = 4.394e-27
Identity = 44/112 (39.29%), Postives = 67/112 (59.82%), Query Frame = 1

Query:   13 KEKFQSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGI----GKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            +E+   G  N +S   +++     +R    +A +  ++ I     + EP  +++ E E  DE IR+W  GKET+IRLLLSTLHH+LW  S W ++ L NL+  SQVK+AYQ+
Sbjct:  298 REEGGGGGGNSDSNDMDEAIAWAKERSQRPEAKQTEEDVIDSRRSEEEPKSEEEMEMEMKDEEIRIWLTGKETNIRLLLSTLHHVLWSNSNWDSIPLANLRDGSQVKKAYQR 409

HSP 2 Score: 51.6026 bits (122), Expect = 4.394e-27
Identity = 23/48 (47.92%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATF-LHKYVGEQAFSILQDAWAAFISED 458
            +K    ARLCLH DKLQQ+G T  + K V  + F+ILQ+AWA +++ +
Sbjct:  404 KKAYQRARLCLHPDKLQQRGGTSPIQKSVASRVFAILQEAWAVYVTNE 451

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT4G12770.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr4:7506736-7511408 REVERSE LENGTH=891)

HSP 1 Score: 62.003 bits (149), Expect = 8.900e-20
Identity = 36/106 (33.96%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK----LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +      +++Q E ++    LD  IR W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  748 GETEERRRARLERHQRTQERAAKALA-EKNERDL----QVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITGASVKKVYRK 848

HSP 2 Score: 52.373 bits (124), Expect = 8.900e-20
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  843 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 890

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT4G12770.2 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr4:7506736-7511408 REVERSE LENGTH=889)

HSP 1 Score: 62.003 bits (149), Expect = 8.901e-20
Identity = 36/106 (33.96%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK----LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +      +++Q E ++    LD  IR W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  746 GETEERRRARLERHQRTQERAAKALA-EKNERDL----QVQREQAEKDRIGGTLDVEIRRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITGASVKKVYRK 846

HSP 2 Score: 52.373 bits (124), Expect = 8.901e-20
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  841 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 888

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT1G21660.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr1:7605924-7608835 FORWARD LENGTH=523)

HSP 1 Score: 63.1586 bits (152), Expect = 1.520e-19
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  + + D  ++T  R   + A+  + +   ++E  ++ +  +E +D  IR W+ GKE ++R LLS+LH +LW   GW  VS+ +L  +S VK+ Y+K
Sbjct:  380 GESEERRKARWDREQRTKSRVAQAVADMNNRDHQSRIEQEQRTRI-SETVDTEIRRWATGKEGNMRALLSSLHIVLWPGCGWEAVSITDLITSSAVKKVYRK 480

HSP 2 Score: 50.447 bits (119), Expect = 1.520e-19
Identity = 23/49 (46.94%), Postives = 33/49 (67.35%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDVS 464
            +K   +A L +H DK+QQKGAT   KY+ E+ F IL++AW  F  E++S
Sbjct:  475 KKVYRKATLYVHPDKVQQKGATLEQKYIAEKVFDILKEAWNKFNKEELS 523

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT4G12780.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr4:7512200-7516655 FORWARD LENGTH=904)

HSP 1 Score: 60.077 bits (144), Expect = 3.305e-19
Identity = 35/106 (33.02%), Postives = 59/106 (55.66%), Query Frame = 1

Query:   31 GSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK----LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            G   E  R + + +++T +R   + A E ++  +      +++Q E ++    LD  I+ W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  761 GETEERRRARLERHQRTQERAAKALA-EKNERDL----QVQREQVEKDRIGVTLDVEIKRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITAASVKKVYRK 861

HSP 2 Score: 52.373 bits (124), Expect = 3.305e-19
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  856 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 903

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT1G75310.1 (| Symbols: AUL1 | auxin-like 1 protein | chr1:28261004-28266124 FORWARD LENGTH=1448)

HSP 1 Score: 66.2402 bits (160), Expect = 1.216e-18
Identity = 35/110 (31.82%), Postives = 64/110 (58.18%), Query Frame = 1

Query:   25 QSGSPNEESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTETEK------LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            Q+G P +  + +++ +++T  R   + A +       K+   K Q+ +TE+      LD +++ WS GKE ++R L+STL +IL  ESGW  + L +L  ++ V++AY+K
Sbjct: 1304 QTGEPIQRCKARSERHQRTSDRAAEALAEK-------KLRDLKTQKEQTERNRLAEALDADVKRWSSGKENNLRALISTLQYILGAESGWKPIPLTDLVSSASVRKAYRK 1406

HSP 2 Score: 44.2838 bits (103), Expect = 1.216e-18
Identity = 19/46 (41.30%), Postives = 31/46 (67.39%), Query Frame = 3

Query:  321 KGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISED 458
            K   +A L +H DKLQQ+GA+   KY+ E+ F +L++AW  F +++
Sbjct: 1402 KAYRKATLYVHPDKLQQRGASTQQKYICEKVFDLLKEAWNKFGADE 1447

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT4G12780.2 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr4:7512200-7516655 FORWARD LENGTH=894)

HSP 1 Score: 57.3806 bits (137), Expect = 2.073e-18
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 1

Query:  178 LDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            LD  I+ W  GKE ++R LLSTL ++LW E GW  VSL +L   + VK+ Y+K
Sbjct:  799 LDVEIKRWGAGKEGNLRALLSTLQYVLWPECGWQPVSLTDLITAASVKKVYRK 851

HSP 2 Score: 52.373 bits (124), Expect = 2.073e-18
Identity = 22/48 (45.83%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISEDV 461
            +K   +A LC+H DK+QQKGA    KY+ E+ F +L++AW  F SE++
Sbjct:  846 KKVYRKATLCIHPDKVQQKGANLQQKYIAEKVFDMLKEAWNKFNSEEL 893

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT1G75100.1 (| Symbols: JAC1 | J-domain protein required for chloroplast accumulation response 1 | chr1:28191108-28193769 REVERSE LENGTH=651)

HSP 1 Score: 62.003 bits (149), Expect = 2.713e-18
Identity = 29/67 (43.28%), Postives = 42/67 (62.69%), Query Frame = 1

Query:  136 KVEPPKKQQTETEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            K+E   K   E + +D  IR WS GK  +IR LLSTL +ILW  SGW  V L+++   + V+++YQ+
Sbjct:  542 KMEEANKDAEEIKNIDAKIRKWSSGKSGNIRSLLSTLQYILWSGSGWKPVPLMDMIEGNAVRKSYQR 608

HSP 2 Score: 47.3654 bits (111), Expect = 2.713e-18
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 3

Query:  321 KGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAF 446
            K    A L LH DKLQQKGA+   KY+ E+ F +LQ+AW  F
Sbjct:  604 KSYQRALLILHPDKLQQKGASANQKYMAEKVFELLQEAWDHF 645

[Close]

BLAST of FG533682 vs. TAIR peptide
Match: AT4G36520.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr4:17230589-17235435 REVERSE LENGTH=1422)

HSP 1 Score: 58.5362 bits (140), Expect = 5.863e-18
Identity = 37/116 (31.90%), Postives = 58/116 (50.00%), Query Frame = 1

Query:    1 IAWAKEKFQSGSPNE-ESRMKNDSNEQTVQRQGSSDANEYHDNGIGKVEPPKKQQTE---TEKLDENIRMWSYGKETDIRLLLSTLHHILWQESGWYTVSLVNLKGNSQVKRAYQK 336
            +++   ++Q     E ES  +  S  +  QR     A    +  +  +   ++Q       E LD  ++ WS GKE +IR LLSTL +IL  ESGW  + L  +  ++ VKRAY+K
Sbjct: 1265 VSFGASRYQDSHGTEGESPQRYTSRLERHQRTADRVAKALAEKNMRDLVAQREQAERIRIAETLDTEVKRWSSGKEGNIRALLSTLQYILGPESGWQPLPLTEVITSAAVKRAYRK 1380

HSP 2 Score: 49.6766 bits (117), Expect = 5.863e-18
Identity = 21/46 (45.65%), Postives = 31/46 (67.39%), Query Frame = 3

Query:  318 EKGLSEARLCLHTDKLQQKGATFLHKYVGEQAFSILQDAWAAFISE 455
            ++   +A LC+H DKLQQ+GA    KY+ E+ F +L++AW  F SE
Sbjct: 1375 KRAYRKATLCVHPDKLQQRGANIHQKYICEKVFDLLKEAWNRFNSE 1420

The following BLAST results are available for this feature:

BLAST of FG533682 vs. TrEMBL
BLAST of FG533682 vs. SwissProt
BLAST of FG533682 vs. TAIR peptide

BLAST of FG533682 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T410_RICCO	3.756e-36	44.53	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7TKC2_VITVI	5.284e-35	43.31	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HZP4_POPTR	9.902e-31	70.18	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AZP9_VITVI	2.210e-29	43.31	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9H411_POPTR	1.735e-26	63.64	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7KES8_ARALY	6.056e-26	41.96	Heat shock protein binding protein OS=Arabidopsis ...	[more]
Q9C752_ARATH	6.494e-25	39.29	Putative uncharacterized protein At1g30280 OS=Arab...	[more]
C5Y7H1_SORBI	7.321e-19	34.31	Putative uncharacterized protein Sb05g026150 OS=So...	[more]
C0PDH9_MAIZE	9.523e-19	34.31	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B8A0P4_MAIZE	9.523e-19	34.31	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of FG533682 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
AUXI2_ARATH	8.840e-19	33.96	Auxilin-related protein 2 OS=Arabidopsis thaliana ...	[more]
AUXI1_ARATH	3.283e-18	33.02	Auxilin-related protein 1 OS=Arabidopsis thaliana ...	[more]
UCP7_SCHPO	5.295e-8	34.62	UBA domain-containing protein 7 OS=Schizosaccharom...	[more]

BLAST of FG533682 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT1G30280.1	4.394e-27	39.29	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT4G12770.1	8.900e-20	33.96	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT4G12770.2	8.901e-20	33.96	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT1G21660.1	1.520e-19	32.35	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT4G12780.1	3.305e-19	33.02	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT1G75310.1	1.216e-18	31.82	\| Symbols: AUL1 \| auxin-like 1 protein \| chr1:2826...	[more]
AT4G12780.2	2.073e-18	49.06	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT1G75100.1	2.713e-18	43.28	\| Symbols: JAC1 \| J-domain protein required for ch...	[more]
AT4G36520.1	5.863e-18	31.90	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	Heat shock protein DnaJ, N-terminal	GENE3D	1.10.287.110	no description	coord: 28..113 score: 1.6
IPR001623	Heat shock protein DnaJ, N-terminal	SUPERFAMILY	46565	Chaperone J-domain	coord: 18..112 score: 2
None	No IPR available	PANTHER	PTHR23172	AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED	coord: 15..112 score: 2.5
None	No IPR available	PANTHER	PTHR23172:SF19	AUXILIN	coord: 15..112 score: 2.5
None	No IPR available	PANTHER	PTHR23172	AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED	coord: 39..79 score: 3.5
None	No IPR available	PANTHER	PTHR23172:SF19	AUXILIN	coord: 39..79 score: 3.5
None	No IPR available	SEG	seg	seg	coord: 9..20 score: NAcoord: 36..43 score
None	No IPR available	SEG	seg	seg	coord: 28..39 score: NAcoord: 47..58 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG533682 ID=FG533682; Name=FG533682; organism=Pisum sativum; type=EST; length=688bp
ATAGCATGGGCCAAAGAGAAGTTTCAGTCAGGTAGCCCTAACGAAGAGTC
AAGAATGAAAAACGATAGCAATGAGCAGACTGTTCAAAGACAAGGAAGCT
CTGATGCAAATGAATACCATGATAATGGAATTGGGAAAGTTGAACCTCCT
AAGAAGCAACAGACAGAAACAGAGAAGTTGGATGAAAACATAAGAATGTG
GTCATATGGCAAAGAAACTGACATCAGGCTACTGCTCTCAACACTACACC
ATATTCTATGGCAGGAGAGTGGCTGGTATACGGTTTCTCTTGTGAATCTA
AAAGGAAACTCACAGGTGAAAAGGGCTTATCAGAAGCAAGGTTATGTCTA
CATACAGACAAACTACAACAGAAAGGCGCAACATTCCTGCACAAGTATGT
AGGAGAGCAAGCTTTTTCCATTCTTCAGGATGCATGGGCTGCATTCATTT
CTGAAGATGTTTCAATTTGACATCAGTCATTGATGGCTCAGTTTTGTCTT
GAAAGTTGTGGACAAACACCAGGCTCTACATTTGGAGATTTTAATACATG
AGCTCCTTTGTACAAATAGTTACTGATGGATGGATCCATTATTTTTGTCT
ATGCGTACTATCATAATGTTATTTGATGCTTTAGTAGGTAGTAGTATTAT
TTTTGAAGTAGTTAATATGAAATAATTTGATTGGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0031072	heat shock protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001623	DnaJ_domain

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