FG535745

EST Overview


Name	FG535745
Unique Name	FG535745
Type	EST
Organism	Pisum sativum (pea)
Sequence length	640

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG535745 vs. TrEMBL
Match: B9S6F5_RICCO (DNA-3-methyladenine glycosylase, putative OS=Ricinus communis GN=RCOM_0536110 PE=4 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 1.944e-48
Identity = 105/207 (50.72%), Postives = 132/207 (63.77%), Query Frame = 2

Query:   20 SKIPFPVKKVPKLTIKTKQXXXXXXXXXXXXXXXXXXSSHGELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            +KIPF  +K+ KL+I T                    S+ GE+  AL HLR+++PLL+ +INT   P F +N  PF               AA SI  RF++L   +S +LP+ VLS S   LR++GISG KA+Y+HDL+ KY NG LSDSSILEM D+ L   LT+VKGIG WSVHMFMIF+LHRPDVLPVGDL VR+GV+ LYGL
Sbjct:   36 TKIPFRPRKIRKLSIHTSTANNTEASSAGKLL-----SAKGEIDLALEHLRNSEPLLAALINTHKHPIFESNTPPFLSLSRSILYQQLALNAAKSIYTRFLTLCGGESGVLPENVLSLSVQQLREIGISGRKASYLHDLADKYRNGSLSDSSILEMSDDMLLTSLTAVKGIGVWSVHMFMIFSLHRPDVLPVGDLGVRKGVQSLYGL 237

[Close]

BLAST of FG535745 vs. TrEMBL
Match: D7SPD0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_23.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018948001 PE=4 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 9.649e-48
Identity = 102/213 (47.89%), Postives = 135/213 (63.38%), Query Frame = 2

Query:    2 IKTLTSSKIPFPVKKVPKLTIKTKQXXXXXXXXXXXXXXXXXXSSHGELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            ++  +SSK+PF  +K+ K++                       S  GEL  AL HL  +DPLL+ +INT   P F +   PF              KAA+SI  RF++L   ++ ++PDAVL+ SP+ LRQ+G+SG KA Y+HDL+SKY  G LSDSSI+ MDD++L+  LT VKGIG WSVHMFMIF+LHRPDVLPVGD+ VR+GV+ LYGL
Sbjct:   39 LQASSSSKLPFRSRKIRKISSAATPSGSDGKSEPPL-------SCEGELDVALRHLTKSDPLLAALINTHQPPTFDSCHPPFLALAKSILYQQLAYKAATSIYTRFVALCGGEAGVVPDAVLALSPSQLRQIGVSGRKAGYLHDLASKYKTGILSDSSIMGMDDKSLFTMLTMVKGIGSWSVHMFMIFSLHRPDVLPVGDVGVRKGVQFLYGL 244

[Close]

BLAST of FG535745 vs. TrEMBL
Match: A5C2I9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009035 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 8.169e-47
Identity = 95/170 (55.88%), Postives = 120/170 (70.59%), Query Frame = 2

Query:  131 SSHGELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            S  GEL  AL HL  +DPLL+ +INT   P F +   PF              KAA+SI  RF++L   ++ ++PDAVL+ SP+ LRQ+G+SG KA Y+HDL+SKY  G LSDSSI+ MDD++L+  LT VKGIG WSVHMFMIF+LHRPDVLPVGD+ VR+GV+ LYGL
Sbjct:  105 SCEGELDVALRHLTKSDPLLAALINTHQPPTFDSCHPPFLALAKSILYQQLAYKAATSIYTRFVALCGGEAGVVPDAVLALSPSQLRQIGVSGRKAGYLHDLASKYKTGILSDSSIMGMDDKSLFTMLTMVKGIGSWSVHMFMIFSLHRPDVLPVGDVGVRKGVQFLYGL 274

[Close]

BLAST of FG535745 vs. TrEMBL
Match: B9R7V3_RICCO (DNA-3-methyladenine glycosylase, putative OS=Ricinus communis GN=RCOM_1594450 PE=4 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.393e-46
Identity = 92/170 (54.12%), Postives = 120/170 (70.59%), Query Frame = 2

Query:  131 SSHGELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            S  GE+++A+ HLR ADPLLS++I+  P P F T  TPF              KA +SI  RFISL   ++ ++PD VL+ +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+I+ MDD++L+  LT V GIG WSVHMFMIF+LHRPDVLP+ DL VR+GV+ LY L
Sbjct:  121 SCEGEVENAIRHLREADPLLSSLIDLHPPPTFDTFHTPFLALTRSILYQQLAFKAGTSIYTRFISLCGGEAGVVPDTVLALTPQQLRQIGVSGRKASYLHDLARKYHNGILSDSAIVNMDDKSLFTMLTMVNGIGSWSVHMFMIFSLHRPDVLPINDLGVRKGVQLLYNL 290

[Close]

BLAST of FG535745 vs. TrEMBL
Match: B9GRN1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_643115 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 3.104e-46
Identity = 90/167 (53.89%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL+ A+ HLR+ADPLL+++I+ +P P F T  TPF              KA +SI  RFISL   ++ +LP+ VL+ +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+I+ MDD++L+  LT V GIG WSVHMFMIF+LHRPDVLP+ DL VR+G++ LY L
Sbjct:  133 GELEIAIRHLRNADPLLASLIDIYPPPTFDTFPTPFLALARSILYQQLAFKAGTSIYTRFISLCGGEAGVLPETVLALTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAIVNMDDKSLFTMLTMVNGIGSWSVHMFMIFSLHRPDVLPINDLQVRKGLQVLYNL 299

[Close]

BLAST of FG535745 vs. TrEMBL
Match: B9H8Q8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1079396 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 4.482e-45
Identity = 90/167 (53.89%), Postives = 119/167 (71.26%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL+ A+++LR+ADPLL+++I+ +  P F T  TPF              KA SSI  RFISL   ++ +LP+ VL+ +P  LRQ G+SG KA+Y+HDL+ KY NG LSDS+I+ MDD++L+  LT V GIG WSVHMFMIF+LHRPDVLP+ DL VR+GV+ LY L
Sbjct:  129 GELEYAIHYLRNADPLLASLIDIYQPPSFDTFPTPFLALARSILYQQLAFKAGSSIYTRFISLCGGEAGVLPETVLALTPQQLRQFGVSGRKASYLHDLARKYQNGILSDSAIVNMDDKSLFTMLTMVNGIGSWSVHMFMIFSLHRPDVLPINDLQVRKGVQLLYNL 295

[Close]

BLAST of FG535745 vs. TrEMBL
Match: Q9LN45_ARATH (F18O14.25 OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 9.985e-45
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. TrEMBL
Match: Q8LAV6_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 9.985e-45
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. TrEMBL
Match: Q56YU8_ARATH (Putative uncharacterized protein At1g19480 OS=Arabidopsis thaliana GN=At1g19480 PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 9.985e-45
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. TrEMBL
Match: Q0V7V4_ARATH (At1g19480 OS=Arabidopsis thaliana GN=At1g19480 PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 9.985e-45
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. SwissProt
Match: MAG1_SCHPO (DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe GN=mag1 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.916e-13
Identity = 41/119 (34.45%), Postives = 64/119 (53.78%), Query Frame = 2

Query:  287 NKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILE-MDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            +KAA++I NRF S+  N     P+ +       +R  G S  K   +  ++    +G +      E + +E L E+LT +KGIG W+V M +IF+L+R DV+P  DL +R G   L+ L
Sbjct:   65 SKAANAIFNRFKSISNNGQFPTPEEIRDMDFEIMRACGFSARKIDSLKSIAEATISGLIPTKEEAERLSNEELIERLTQIKGIGRWTVEMLLIFSLNRDDVMPADDLSIRNGYRYLHRL 183

[Close]

BLAST of FG535745 vs. SwissProt
Match: YFJP_BACSU (Putative DNA-3-methyladenine glycosylase yfjP OS=Bacillus subtilis GN=yfjP PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 9.444e-11
Identity = 37/120 (30.83%), Postives = 62/120 (51.67%), Query Frame = 2

Query:  296 ASSIENRFISLFQNQSSIL-----PDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            A ++  RF+  F  Q   +     P+ +       LR +  S  KA Y  D S   A G LS S +  M DE + +KL  ++GIGPW+V   ++F L RP++ P+ D+ ++  +++ + L
Sbjct:  136 AYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQDLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPLADIGLQNAIKRHFQL 255

[Close]

BLAST of FG535745 vs. SwissProt
Match: MAG2_SCHPO (Probable DNA-3-methyladenine glycosylase 2 OS=Schizosaccharomyces pombe GN=SPBC23G7.11 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.044e-9
Identity = 46/178 (25.84%), Postives = 80/178 (44.94%), Query Frame = 2

Query:  131 SSHGELQSALNHLRSADPLLSTIINTF------PSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFIS-LFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFL-SDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            S   + + A  HL S D   S+++         P P+ +    P+             + A +SI N+F +    N     P  ++      L + G S  K+  IH ++    N  + S S I +M +E L E L+ +KG+  W++ M+ IFTL R D++P  D  ++   ++ +GL
Sbjct:    2 SKDSDYKRAEKHLSSIDNKWSSLVKKVGPCTLTPHPEHA----PYEGIIRAITSQKLSDAATNSIINKFCTQCSDNDEFPTPKQIMETDVETLHECGFSKLKSQEIHIVAEAALNKQIPSKSEIEKMSEEELMESLSKIKGVKRWTIEMYSIFTLGRLDIMPADDSTLKNEAKEFFGL 175

[Close]

BLAST of FG535745 vs. SwissProt
Match: MAG_YEAST (DNA-3-methyladenine glycosylase OS=Saccharomyces cerevisiae GN=MAG1 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.667e-7
Identity = 38/123 (30.89%), Postives = 58/123 (47.15%), Query Frame = 2

Query:  290 KAASSIENRFISLFQNQSSILPDAVLSFSP-------NHLRQVGISGPKATYIHDLSSKYANGFLSDSSIL---EMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEK 628
            +AA SI+ R +SL+       PD  + F           + + G+S  K  Y+  L+  +   +     +    + D+E +   +T+VKGIGPWS  MF+I  L R DV    DL + RG  K
Sbjct:   99 QAAESIKARVVSLY---GGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYKDIEKLFGQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSK 218

[Close]

BLAST of FG535745 vs. TAIR peptide
Match: AT1G19480.2 (| Symbols: | DNA glycosylase superfamily protein | chr1:6744520-6745653 FORWARD LENGTH=377)

HSP 1 Score: 183.726 bits (465), Expect = 7.107e-47
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. TAIR peptide
Match: AT1G19480.1 (| Symbols: | DNA glycosylase superfamily protein | chr1:6744520-6746144 FORWARD LENGTH=382)

HSP 1 Score: 183.726 bits (465), Expect = 7.107e-47
Identity = 89/167 (53.29%), Postives = 120/167 (71.86%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++A+++LR+ADPLL+ +I+  P P F +  TPF              KA +SI  RF+SL   ++ ++P+ VLS +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS+IL MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ LYGL
Sbjct:  137 GELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQLAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGILSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

[Close]

BLAST of FG535745 vs. TAIR peptide
Match: AT1G75230.1 (| Symbols: | DNA glycosylase superfamily protein | chr1:28234444-28236452 FORWARD LENGTH=391)

HSP 1 Score: 179.489 bits (454), Expect = 1.340e-45
Identity = 87/167 (52.10%), Postives = 117/167 (70.06%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++AL+HLRS DPLL+++I+  P P F T  TPF              KA +SI  RF++L   ++ ++P+ VL  +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS I+ MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ L G+
Sbjct:  133 GELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKAGNSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSGIVNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGM 299

[Close]

BLAST of FG535745 vs. TAIR peptide
Match: AT1G75230.2 (| Symbols: | DNA glycosylase superfamily protein | chr1:28234444-28235628 FORWARD LENGTH=394)

HSP 1 Score: 179.489 bits (454), Expect = 1.340e-45
Identity = 87/167 (52.10%), Postives = 117/167 (70.06%), Query Frame = 2

Query:  140 GELQSALNHLRSADPLLSTIINTFPSPQFSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQNQSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            GEL++AL+HLRS DPLL+++I+  P P F T  TPF              KA +SI  RF++L   ++ ++P+ VL  +P  LRQ+G+SG KA+Y+HDL+ KY NG LSDS I+ MD+++L+  LT V GIG WSVHMFMI +LHRPDVLPV DL VR+GV+ L G+
Sbjct:  133 GELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKAGNSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSGIVNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGM 299

[Close]

BLAST of FG535745 vs. TAIR peptide
Match: AT3G50880.1 (| Symbols: | DNA glycosylase superfamily protein | chr3:18913509-18914330 FORWARD LENGTH=273)

HSP 1 Score: 169.859 bits (429), Expect = 1.062e-42
Identity = 89/172 (51.74%), Postives = 117/172 (68.02%), Query Frame = 2

Query:  131 SSHGELQSALNHLRSADPLLSTIINTFPSPQ-FSTNITPFFXXXXXXXXXXXXNKAASSIENRFISLFQN-QSSILPDAVLSFSPNHLRQVGISGPKATYIHDLSSKYANGFLSDSSILEMDDETLYEKLTSVKGIGPWSVHMFMIFTLHRPDVLPVGDLVVRRGVEKLYGL 640
            S+   +  AL HL+S+D LL  +I T   P  F ++ TPF              KAA  I +RFISLF   ++ ++P++V+S S   LR++G+SG KA+Y+HDL+ KY NG LSD  IL+M DE L ++LT VKGIG W+VHMFMIF+LHRPDVLPVGDL VR+GV+ LYGL
Sbjct:   65 STKSTVDIALRHLQSSDELLGALITTHNDPPLFDSSNTPFLSLARSILYQQLATKAAKCIYDRFISLFNGGEAGVVPESVISLSAVDLRKIGVSGRKASYLHDLADKYNNGVLSDELILKMSDEELIDRLTLVKGIGVWTVHMFMIFSLHRPDVLPVGDLGVRKGVKDLYGL 236

The following BLAST results are available for this feature:

BLAST of FG535745 vs. TrEMBL
BLAST of FG535745 vs. SwissProt
BLAST of FG535745 vs. TAIR peptide

BLAST of FG535745 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S6F5_RICCO	1.944e-48	50.72	DNA-3-methyladenine glycosylase, putative OS=Ricin...	[more]
D7SPD0_VITVI	9.649e-48	47.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5C2I9_VITVI	8.169e-47	55.88	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9R7V3_RICCO	1.393e-46	54.12	DNA-3-methyladenine glycosylase, putative OS=Ricin...	[more]
B9GRN1_POPTR	3.104e-46	53.89	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H8Q8_POPTR	4.482e-45	53.89	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9LN45_ARATH	9.985e-45	53.29	F18O14.25 OS=Arabidopsis thaliana PE=4 SV=1	[more]
Q8LAV6_ARATH	9.985e-45	53.29	Putative uncharacterized protein OS=Arabidopsis th...	[more]
Q56YU8_ARATH	9.985e-45	53.29	Putative uncharacterized protein At1g19480 OS=Arab...	[more]
Q0V7V4_ARATH	9.985e-45	53.29	At1g19480 OS=Arabidopsis thaliana GN=At1g19480 PE=...	[more]

BLAST of FG535745 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
MAG1_SCHPO	5.916e-13	34.45	DNA-3-methyladenine glycosylase 1 OS=Schizosacchar...	[more]
YFJP_BACSU	9.444e-11	30.83	Putative DNA-3-methyladenine glycosylase yfjP OS=B...	[more]
MAG2_SCHPO	1.044e-9	25.84	Probable DNA-3-methyladenine glycosylase 2 OS=Schi...	[more]
MAG_YEAST	1.667e-7	30.89	DNA-3-methyladenine glycosylase OS=Saccharomyces c...	[more]

BLAST of FG535745 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G19480.2	7.107e-47	53.29	\| Symbols: \| DNA glycosylase superfamily protein ...	[more]
AT1G19480.1	7.107e-47	53.29	\| Symbols: \| DNA glycosylase superfamily protein ...	[more]
AT1G75230.1	1.340e-45	52.10	\| Symbols: \| DNA glycosylase superfamily protein ...	[more]
AT1G75230.2	1.340e-45	52.10	\| Symbols: \| DNA glycosylase superfamily protein ...	[more]
AT3G50880.1	1.062e-42	51.74	\| Symbols: \| DNA glycosylase superfamily protein ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 2..41 score
None	No IPR available	GENE3D	1.10.340.30	no description	coord: 81..192 score: 2
None	No IPR available	SEG	seg	seg	coord: 26..43 score: NAcoord: 84..95 score
None	No IPR available	SEG	seg	seg	coord: 1..13 score
None	No IPR available	SEG	seg	seg	coord: 25..40 score
None	No IPR available	SEG	seg	seg	coord: 69..80 score
IPR003265	HhH-GPD domain	PFAM	PF00730	HhH-GPD	coord: 86..212 score: 5.3
IPR011257	DNA glycosylase	SUPERFAMILY	48150	DNA-glycosylase	coord: 69..210 score: 7.3

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG535745 ID=FG535745; Name=FG535745; organism=Pisum sativum; type=EST; length=640bp
AATCAAAACCCTAACCTCTTCAAAAATCCCATTCCCCGTTAAAAAAGTCC
CCAAACTCACCATCAAAACCAAACAACCACATCTTCATCTCCCCGTAACA
CCACCACCACCAACAATCCCAAAACCATTATCATCACACGGCGAACTCCA
ATCCGCTCTCAACCACCTTCGCTCCGCCGACCCACTCCTATCAACCATCA
TCAACACTTTCCCTTCCCCACAATTCTCAACCAACATCACACCCTTCTTT
TCTCTCATCAAAAGCATAATCTCCCAACAACTCTCCAACAAAGCCGCATC
CTCGATCGAAAACCGTTTCATCTCTCTCTTTCAGAACCAATCCTCAATCC
TCCCCGACGCCGTTTTATCCTTTTCCCCAAACCACCTTCGCCAGGTCGGA
ATCTCGGGACCGAAAGCAACTTACATTCACGACCTTTCCTCCAAATACGC
TAATGGCTTTCTGTCTGATTCATCGATTCTCGAAATGGATGATGAAACAC
TGTACGAGAAATTAACGTCGGTTAAAGGGATTGGACCTTGGTCGGTTCAT
ATGTTTATGATTTTTACACTTCATCGTCCTGATGTTTTGCCGGTTGGGGA
TCTTGTTGTGAGGAGAGGTGTGGAAAAGTTGTATGGTCTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006284	base-excision repair
GO:0006281	DNA repair

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR003265	HhH-GPD_domain
IPR011257	DNA_glycosylase

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