FG538768

EST Overview


Name	FG538768
Unique Name	FG538768
Type	EST
Organism	Pisum sativum (pea)
Sequence length	599

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG538768 vs. TrEMBL
Match: C6T4J7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 7.551e-86
Identity = 158/192 (82.29%), Postives = 173/192 (90.10%), Query Frame = 2

Query:    2 QQDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQFLQTKILYS 577
            QQDLIY++LHPLLMVIGFILLSGEA+LVHRWLPGS+GLKKL+HLWLQG+AL  GIFGIWTKFQGKD GI+ANFYSLHSWMG   VSLFGAQWLIGF  FWHRGEVR VR  +LPWHVF+GLYTYALA+ATAETGLLEKLTFLQT+RNVPKH+TESMVVN+LGL LAL  GFVILAAVSP  YQ  Q+K+ YS
Sbjct:   46 QQDLIYAVLHPLLMVIGFILLSGEAVLVHRWLPGSRGLKKLVHLWLQGVALASGIFGIWTKFQGKD-GIVANFYSLHSWMGLACVSLFGAQWLIGFLDFWHRGEVRSVRIRILPWHVFLGLYTYALAIATAETGLLEKLTFLQTKRNVPKHSTESMVVNSLGLGLALVGGFVILAAVSP-KYQTHQSKLWYS 235

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BLAST of FG538768 vs. TrEMBL
Match: B9HWN0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_822158 PE=4 SV=1)

HSP 1 Score: 286.189 bits (731), Expect = 1.208e-75
Identity = 136/179 (75.98%), Postives = 159/179 (88.83%), Query Frame = 2

Query:    2 QQDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSP 538
            Q+DL+Y++LHPLLMVIGFIL+SGEAILVHRWLPGS+G KK +HL LQGLAL  GIFGIWTKF G +DGI+ANF+SLHSWMG   +SLFGAQWL+GF  FWHRGE+R VR  +LPWHVF+GLYTY LAVATAETGLLEK+TFLQT+++  KH++ESMVVN+LGLSLAL SG +ILAAVSP
Sbjct:   45 QEDLVYAVLHPLLMVIGFILISGEAILVHRWLPGSRGFKKSVHLCLQGLALACGIFGIWTKFHG-NDGIVANFFSLHSWMGLICISLFGAQWLMGFRSFWHRGEMRTVRIRVLPWHVFLGLYTYGLAVATAETGLLEKITFLQTKKDASKHSSESMVVNSLGLSLALLSGIIILAAVSP 222

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BLAST of FG538768 vs. TrEMBL
Match: D7T9A8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011969001 PE=4 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.336e-74
Identity = 138/192 (71.88%), Postives = 160/192 (83.33%), Query Frame = 2

Query:    2 QQDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQFLQTKILYS 577
            Q+D++Y++LHPL MVIGFIL+SGEAILVHRWLPGS+ LKK +HL +QGLAL  G+FGIWT F G+D GI+ANF+SLHSWMG   + LFGAQWLIGF  FWHRGE+R  R  +LPWHVF+GLYTY LAVATAETGLLEKLTFLQT RNV KH TESMVVN +G+ LAL SG VILAAV P  YQ L +K++YS
Sbjct:   46 QEDIVYAVLHPLFMVIGFILISGEAILVHRWLPGSRNLKKSVHLSMQGLALASGVFGIWTNFHGQD-GIVANFWSLHSWMGLICMLLFGAQWLIGFLSFWHRGEMRTTRVSVLPWHVFLGLYTYGLAVATAETGLLEKLTFLQTNRNVSKHCTESMVVNGVGVGLALLSGIVILAAVCP-KYQTLPSKLMYS 235

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BLAST of FG538768 vs. TrEMBL
Match: B9HIX8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820600 PE=4 SV=1)

HSP 1 Score: 275.789 bits (704), Expect = 1.633e-72
Identity = 139/213 (65.26%), Postives = 166/213 (77.93%), Query Frame = 2

Query:    2 QQDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQ---------------------WLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQFLQTKILYS 577
            Q+DLIY++LHPLLMV GFIL+SGEAILVH+WLPGS+G KK +HL LQGLAL  GIFGIWTKF G +DGI+ANF+SLHSWMG   +SLFGAQ                     WL+GF  FWHRGE+R VR  +LPWH+F+GLYTY LAVATAETGLLEK+TFLQT+++V KH+ ESMVVN+LGLSLAL SG +ILAAVSP  YQ  ++++ YS
Sbjct:   45 QEDLIYAVLHPLLMVNGFILISGEAILVHKWLPGSRGFKKSVHLCLQGLALACGIFGIWTKFHG-NDGIVANFFSLHSWMGLICISLFGAQVLEKPLVIWPSVDRCSSHLAGWLMGFMSFWHRGEMRAVRITVLPWHIFLGLYTYGLAVATAETGLLEKITFLQTKKDVSKHSPESMVVNSLGLSLALLSGIIILAAVSP-GYQSFRSELTYS 255

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BLAST of FG538768 vs. TrEMBL
Match: A5B730_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020007 PE=4 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 2.786e-72
Identity = 135/185 (72.97%), Postives = 154/185 (83.24%), Query Frame = 2

Query:    2 QQDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQFL 556
            Q+D++Y++LHPL MVIGFIL+SGEAILVHRWLPGS+ LKK +HL +QGLAL  G+FGIWT F G+D GI+ANF+SLHSWMG   + LFGAQWLIGF  FWHRGE+R  R  +LPWHVF+GLYTY LAVATAETGLLEKLTFLQT RNV KH TESMVVN +G+ LAL SG VILAAV P  YQ L
Sbjct:   46 QEDIVYAVLHPLFMVIGFILISGEAILVHRWLPGSRNLKKSVHLSMQGLALASGVFGIWTNFHGQD-GIVANFWSLHSWMGLICMLLFGAQWLIGFLSFWHRGEMRTTRVSVLPWHVFLGLYTYGLAVATAETGLLEKLTFLQTNRNVSKHCTESMVVNGVGVGLALLSGIVILAAVCP-KYQTL 228

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BLAST of FG538768 vs. TrEMBL
Match: D7KQT9_ARALY (Cytochrome B561 family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_313371 PE=4 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 4.917e-69
Identity = 133/194 (68.56%), Postives = 149/194 (76.80%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQFL--QTKILYSS 580
            Q L YS LHPLLMVIGFIL+SGEAIL+HRWLPGS+  KK +HLWLQG+AL   +FGIWTKF  +  G+ ANFYSLHSWMG   VSLF AQW+ GF  FWHRGEVR  R   LPWHVF+GLYTY LA+ATAETGLLEKLTFLQT+RNVP+  +ESM VN LGL LAL  G VI AAV P  YQ      K++YSS
Sbjct:   36 QGLSYSTLHPLLMVIGFILVSGEAILIHRWLPGSRKTKKAVHLWLQGVALASAVFGIWTKFHYQR-GVFANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFIGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALLCGIVISAAVLP-KYQSHSGNEKLVYSS 227

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BLAST of FG538768 vs. TrEMBL
Match: Q9C540_ARATH (At1g26100 OS=Arabidopsis thaliana GN=F14G11.7 PE=2 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.211e-67
Identity = 130/192 (67.71%), Postives = 147/192 (76.56%), Query Frame = 2

Query:   11 LIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQ--FLQTKILYSS 580
            L YS LHPLLMVIGFIL+SGEAIL+HRWLPGS+  KK +HLWLQG+AL   +FGIWTKF  +  G+ ANFYSLHSWMG   VSLF AQW+ GF  FWHRGEVR  R   LPWHVF+GLYTY LA+ATAETGLLEKLTFLQT+RNVP+  +ESM VN LGL LAL    VI AA+ P  YQ      K++YSS
Sbjct:   38 LSYSTLHPLLMVIGFILVSGEAILIHRWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQR-GVFANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALLGCIVITAAILP-KYQSHSRDEKLVYSS 227

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BLAST of FG538768 vs. TrEMBL
Match: D5ADY3_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 8.427e-53
Identity = 102/180 (56.67%), Postives = 135/180 (75.00%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNN 544
            ++LI+++ HP LM+IGFILLSGEA+LV++ +PG+K  KK +HL LQGLAL   + GIW  ++  +D  + NFYSLHSW+G   V LFG QW +GF+ FW+ G  R  R  +LPWH F+GLY Y LAVATAETGLLEKLTFLQ  + + +++TE+++VN LGL LAL SG VIL+ +SP N
Sbjct:   43 KNLIFNV-HPTLMLIGFILLSGEAMLVYKTVPGTKNYKKAVHLVLQGLALGLAMIGIWAAYKFHNDKGIDNFYSLHSWLGVACVLLFGIQWAVGFATFWYPGGSRNSRATVLPWHSFLGLYIYGLAVATAETGLLEKLTFLQASKTIARYSTEALLVNCLGLLLALLSGCVILSTISPAN 221

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BLAST of FG538768 vs. TrEMBL
Match: B9N202_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_743692 PE=4 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 8.162e-48
Identity = 90/180 (50.00%), Postives = 129/180 (71.67%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNN 544
            +DLI+++ HP+LMVIG +L++GEA+L ++ +PG+K  KKL+HL LQ LA C  + G W   +  +D  + NFYSLHSW+G   + LF  QW  GF  FW+ G  R  R  +LPWHVF G+Y YAL+VATA TG+LEK+TFLQT   + +++TE+++VN+LG+ + +  GFV+LA+VS  N
Sbjct:   44 KDLIFNV-HPVLMVIGLVLVNGEAMLAYKTVPGTKSFKKLVHLTLQFLAFCLSLIGFWAALKFHNDKGIDNFYSLHSWLGLACLFLFSIQWASGFVTFWYPGGSRNSRATLLPWHVFSGVYIYALSVATATTGILEKVTFLQTNNVISRYSTEALLVNSLGILMIVLGGFVVLASVSSLN 222

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BLAST of FG538768 vs. TrEMBL
Match: A5AR35_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_001339 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.375e-47
Identity = 90/180 (50.00%), Postives = 129/180 (71.67%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNN 544
            +DLI+++ HP+LMVIG ILL+GEA+L ++ + G+K  KKL+HL LQ LA C  + G+W   +  ++  + NFYSLHSW+G   + LFG QW  GF+ FW+ G  R  R  +LPWHVF  +Y YALAVATA TG+LEK TFLQ+ + + +++ E+++VN+LG+ + +  GFVILA V+P N
Sbjct:   43 KDLIFNV-HPVLMVIGLILLNGEAMLAYKTVSGTKNYKKLVHLSLQFLAFCLSLVGVWAAVKFHNERGIDNFYSLHSWLGLACLFLFGIQWATGFATFWYPGGSRNSRASLLPWHVFFAVYIYALAVATATTGILEKATFLQSNKVISRYSMEALLVNSLGILIVVLGGFVILAVVTPAN 221

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BLAST of FG538768 vs. SwissProt
Match: CYBR1_XENLA (Cytochrome b reductase 1 OS=Xenopus laevis GN=cybrd1 PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 2.318e-21
Identity = 63/174 (36.21%), Postives = 92/174 (52.87%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHRWLPG----SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSP 538
            HP+L+  GFI + G AI+V+R LP     SK L K +H  L   AL F I  +   F   +   + N YSLHSW+G T V L+  Q ++G S +        +R  ++P HV+ GL+ +   +ATA  G+ EKL F   +    K   E++ VNT GL + +F G V+    +P
Sbjct:   48 HPVLVTSGFIFIQGIAIIVYR-LPWTWKCSKLLMKFIHAGLHLTALIFTIVALVAVFDFHNAKNIPNMYSLHSWIGLTVVILYALQLVLGVSIYLLPFASNTLRAALMPVHVYSGLFIFGTVIATALMGITEKLIFSLKEPPYSKLPPEAIFVNTFGLLILVFGGLVVWMVTTP 220

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BLAST of FG538768 vs. SwissProt
Match: CYBR1_DANRE (Cytochrome b reductase 1 OS=Danio rerio GN=cybrd1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.318e-21
Identity = 63/171 (36.84%), Postives = 91/171 (53.22%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHRWLPG----SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAA 529
            HPLLMVIGFI L G AI+V+R LP     SK + KL+H  L  LA    +  +   F   +   + N YSLHSW+G   V L+ +Q ++G + +       RVR  ++P H++ GL+ +   +A A  G+ EKL F            E+++VN LGL +A F   V+  A
Sbjct:   48 HPLLMVIGFIFLQGIAIVVYR-LPWTWRCSKQMMKLIHAGLHILAFILAVISVVAVFVFHNAKNIPNMYSLHSWVGLAAVVLYPSQIVLGIAVYLIPVTPVRVRAALMPLHIYSGLFIFISVIAAALMGITEKLIFSLKSPAYKDSPPEAVLVNVLGLLIAAFGALVVWIA 217

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BLAST of FG538768 vs. SwissProt
Match: CY561_SHEEP (Cytochrome b561 OS=Ovis aries GN=CYB561 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.318e-21
Identity = 64/166 (38.55%), Postives = 86/166 (51.81%), Query Frame = 2

Query:   26 LHPLLMVIGFILLSGEAILVHRWLPG-SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVI 520
            +HPL MVIG + L G+A+LV+R     +K   K++H  L   A    + G+   F+       A+ YSLHSW G    +LF AQWL+GFS F   G    +R    P HVF G   + L+VATA  GL E L F +          E ++ N LGL LA F+  V+
Sbjct:   53 VHPLCMVIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLF-ELGTKYSTFEPEGVLANVLGLLLAAFATVVL 217

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BLAST of FG538768 vs. SwissProt
Match: CY561_BOVIN (Cytochrome b561 OS=Bos taurus GN=CYB561 PE=1 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 3.954e-21
Identity = 62/166 (37.35%), Postives = 86/166 (51.81%), Query Frame = 2

Query:   26 LHPLLMVIGFILLSGEAILVHRWLPG-SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVI 520
            +HPL M+IG + L G+A+LV+R     +K   K++H  L   A    + G+   F+       A+ YSLHSW G    +LF AQWL+GFS F   G    +R    P HVF G   + L+VATA  GL E L F +          E ++ N LGL LA F+  ++
Sbjct:   53 VHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLVGFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLF-ELGTKYSMFEPEGVLANVLGLLLATFATVIL 217

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BLAST of FG538768 vs. SwissProt
Match: CYBR1_HUMAN (Cytochrome b reductase 1 OS=Homo sapiens GN=CYBRD1 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.150e-20
Identity = 62/174 (35.63%), Postives = 86/174 (49.43%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHRWLPG----SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSP 538
            HP+LMV GF+ + G AI+V+R LP     SK L K +H  L  +A    I  +   F+  +   +AN YSLHSW+G   V  +  Q L GFS F        +R  ++P HV+ G+  +   +ATA  GL EKL F            E + VNTLGL + +F   +      P
Sbjct:   50 HPVLMVTGFVFIQGIAIIVYR-LPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRP 222

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BLAST of FG538768 vs. SwissProt
Match: CY561_PONAB (Cytochrome b561 OS=Pongo abelii GN=CYB561 PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.962e-20
Identity = 63/165 (38.18%), Postives = 83/165 (50.30%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHRWLPG-SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVI 520
            HPL MVIG I L G+A+LV+R     +K   K++H  L   AL   + G+   F        A+ YSLHSW G     L+  QWL+GFS F   G    +R    P H+F G   + L+V TA  GL E L F + +        E ++ N LGL LA F G V+
Sbjct:   53 HPLCMVIGLIFLQGDALLVYRVFRNEAKRTTKVLHGLLHIFALVIALVGLVAVFDYHRKEGYADLYSLHSWCGILVFVLYFVQWLVGFSFFLFPGASFSLRSRYRPQHIFFGATIFLLSVGTALLGLKEALLF-KLRDKYSAFEPEGVLANVLGLLLACFGGAVL 216

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BLAST of FG538768 vs. SwissProt
Match: CYAC3_HUMAN (Cytochrome b ascorbate-dependent protein 3 OS=Homo sapiens GN=CYBASC3 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.563e-20
Identity = 63/187 (33.69%), Postives = 100/187 (53.48%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHR----WLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHN--TESMVVNTLGLSLALFSGFVILAAVSPNNYQFLQTKIL 571
            HP+LMV G ++  G A LV+R    W+ G K   KL+H  L  +A    + G+   F   + G  AN YSLHSW+G T V LF  QW +GF+ F        +R ++ P HVF G    +L++A+  +G+ EKL F       P H+  +E++  N+ G+ +  F G ++L  +  ++++  +  IL
Sbjct:   47 HPVLMVAGMVVFYGGASLVYRLPQSWV-GPKLPWKLLHAALHLMAFVLTVVGLVAVFTFHNHGRTANLYSLHSWLGITTVFLFACQWFLGFAVFLLPWASMWLRSLLKPIHVFFGAAILSLSIASVISGINEKLFFSLKNTTRPYHSLPSEAVFANSTGMLVVAF-GLLVLYILLASSWKRPEPGIL 231

[Close]

BLAST of FG538768 vs. SwissProt
Match: CYBR1_XENTR (Cytochrome b reductase 1 OS=Xenopus tropicalis GN=cybrd1 PE=2 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 3.347e-20
Identity = 62/174 (35.63%), Postives = 90/174 (51.72%), Query Frame = 2

Query:   29 HPLLMVIGFILLSGEAILVHRWLPG----SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSP 538
            HP+L+  GFI + G AI+V+R LP     SK L K +H  L   A  F I  +   F   +   + N YSLHSW+G T V L+  Q ++G S +        +R  ++P HV+ GL  +   +ATA  G+ EKL F   +    K   E++ VNT GL + +F G V+    +P
Sbjct:   48 HPVLVTSGFIFIQGIAIIVYR-LPWTWNCSKLLMKFIHAGLHLTAFVFTIVALVAVFDFHNAKNIPNMYSLHSWIGLTVVILYALQLVLGVSIYLLPFARDTLRAALMPVHVYSGLLIFGTVIATALMGITEKLIFSLKEPPYSKMPPEAIFVNTFGLIILVFGGLVVWMVTTP 220

[Close]

BLAST of FG538768 vs. SwissProt
Match: CY561_PIG (Cytochrome b561 OS=Sus scrofa GN=CYB561 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 5.709e-20
Identity = 61/166 (36.75%), Postives = 82/166 (49.40%), Query Frame = 2

Query:   26 LHPLLMVIGFILLSGEAILVHRWLPG-SKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVI 520
            +HPL M+IG + L G+A+LV+R     +K   K++H  L  LA    + G+   F       +A+ YSLHSW G     LF AQWL+G   F   G    +R    P HVF G   + L+V TA  GL E L F Q         +E ++ N LGL L  F   V+
Sbjct:   53 VHPLCMIIGLVFLQGDALLVYRVFRNEAKRTTKILHGLLHVLAFVIALVGLVAVFDYHRKKGIADLYSLHSWCGILVFVLFLAQWLVGLGFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVGTALLGLKEALLF-QLGTKYSAFESEGVLANVLGLLLVAFGAVVL 217

[Close]

BLAST of FG538768 vs. SwissProt
Match: CY561_XENLA (Cytochrome b561 OS=Xenopus laevis GN=cyb561 PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 9.738e-20
Identity = 57/165 (34.55%), Postives = 85/165 (51.52%), Query Frame = 2

Query:   26 LHPLLMVIGFILLSGEAILVHR-WLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFV 517
            +HPL MV+G + L GEA+LV+R +   +K   K++H  L  +AL   + G+   FQ        + YSLHSW G    +L+  QW+IGFS F+  G     R    P H F G   +  ++AT+  GL EK+           H  E ++VN+LG+ L +F   +
Sbjct:   52 VHPLCMVLGMVFLCGEALLVYRVFRHETKRSTKILHGVLHIMALVISLVGVIAVFQYHQANGYPDMYSLHSWCGIVTFTLYILQWIIGFSLFFIPGVAFTYRSQFKPLHEFFGRALFLSSIATSLLGLTEKMF-----SEYSSHPAEGILVNSLGVLLVVFGAVI 211

[Close]

BLAST of FG538768 vs. TAIR peptide
Match: AT1G26100.1 (| Symbols: | Cytochrome b561/ferric reductase transmembrane protein family | chr1:9022716-9024081 REVERSE LENGTH=236)

HSP 1 Score: 259.61 bits (662), Expect = 9.087e-70
Identity = 130/192 (67.71%), Postives = 147/192 (76.56%), Query Frame = 2

Query:   11 LIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSPNNYQ--FLQTKILYSS 580
            L YS LHPLLMVIGFIL+SGEAIL+HRWLPGS+  KK +HLWLQG+AL   +FGIWTKF  +  G+ ANFYSLHSWMG   VSLF AQW+ GF  FWHRGEVR  R   LPWHVF+GLYTY LA+ATAETGLLEKLTFLQT+RNVP+  +ESM VN LGL LAL    VI AA+ P  YQ      K++YSS
Sbjct:   38 LSYSTLHPLLMVIGFILVSGEAILIHRWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQR-GVFANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALLGCIVITAAILP-KYQSHSRDEKLVYSS 227

[Close]

BLAST of FG538768 vs. TAIR peptide
Match: AT5G38630.1 (| Symbols: ACYB-1, CYB-1 | cytochrome B561-1 | chr5:15466141-15467511 FORWARD LENGTH=230)

HSP 1 Score: 176.407 bits (446), Expect = 1.012e-44
Identity = 82/178 (46.07%), Postives = 122/178 (68.54%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAAVSP 538
            +D I+++ HP++MVIG IL +GEA+L ++ + G+K LKKL+HL LQ  A    + G+W   +   D  + NFYSLHSW+G   + LF  QW  GF  +W+ G  R  R  ++PWHVF+G+  YALA+ TA TG+LEK+TFLQ  + + +++TE+M+VNT+G+ + +  GFVIL  V+P
Sbjct:   44 KDHIFNV-HPVMMVIGLILFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTP 220

[Close]

BLAST of FG538768 vs. TAIR peptide
Match: AT4G25570.1 (| Symbols: ACYB-2 | Cytochrome b561/ferric reductase transmembrane protein family | chr4:13053887-13055518 REVERSE LENGTH=239)

HSP 1 Score: 153.68 bits (387), Expect = 7.023e-38
Identity = 77/175 (44.00%), Postives = 113/175 (64.57%), Query Frame = 2

Query:    5 QDLIYSILHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALFSGFVILAA 529
            ++LI++ LHP+LM+IGFI+L GEAI+ ++ LP  K +KKL+HL L  +AL  GIFGI   F+  ++  + N YSLHSW+G   +SL+G QW+  F  F+  G    ++  +LPWH  +GL+ Y LAV  A  G LEKLTFL+    + K+ +E+ ++N   +   LF  FV+L A
Sbjct:   43 KNLIFN-LHPVLMLIGFIILGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE-NGGLDKYGSEAFLINFTAIITILFGAFVVLTA 215

[Close]

BLAST of FG538768 vs. TAIR peptide
Match: AT1G14730.1 (| Symbols: | Cytochrome b561/ferric reductase transmembrane protein family | chr1:5073244-5074568 FORWARD LENGTH=224)

HSP 1 Score: 101.679 bits (252), Expect = 3.167e-22
Identity = 57/160 (35.62%), Postives = 82/160 (51.25%), Query Frame = 2

Query:   26 LHPLLMVIGFILLSGEAILVHRWLPGSKGLKKLMHLWLQGLALCFGIFGIWTKFQGKDDGILANFYSLHSWMGFTRVSLFGAQWLIGFSKFWHRGEVRRVRKMMLPWHVFVGLYTYALAVATAETGLLEKLTFLQTQRNVPKHNTESMVVNTLGLSLALF 505
            +HP LM  GF+ L G+A++ ++    S  ++K++H  L  + L  GI GI   F+  D   L +  SLHSW+G T   L G QWL G   F         R  M+PWHV  G     + +  A TGL+++ T L         N ES ++N LGL++ LF
Sbjct:   56 VHPFLMYCGFLFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQST-----NAESRLINFLGLAILLF 210

The following BLAST results are available for this feature:

BLAST of FG538768 vs. TrEMBL
BLAST of FG538768 vs. SwissProt
BLAST of FG538768 vs. TAIR peptide

BLAST of FG538768 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T4J7_SOYBN	7.551e-86	82.29	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9HWN0_POPTR	1.208e-75	75.98	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T9A8_VITVI	1.336e-74	71.88	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HIX8_POPTR	1.633e-72	65.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5B730_VITVI	2.786e-72	72.97	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7KQT9_ARALY	4.917e-69	68.56	Cytochrome B561 family protein OS=Arabidopsis lyra...	[more]
Q9C540_ARATH	1.211e-67	67.71	At1g26100 OS=Arabidopsis thaliana GN=F14G11.7 PE=2...	[more]
D5ADY3_PICSI	8.427e-53	56.67	Putative uncharacterized protein OS=Picea sitchens...	[more]
B9N202_POPTR	8.162e-48	50.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AR35_VITVI	2.375e-47	50.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of FG538768 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CYBR1_XENLA	2.318e-21	36.21	Cytochrome b reductase 1 OS=Xenopus laevis GN=cybr...	[more]
CYBR1_DANRE	2.318e-21	36.84	Cytochrome b reductase 1 OS=Danio rerio GN=cybrd1 ...	[more]
CY561_SHEEP	2.318e-21	38.55	Cytochrome b561 OS=Ovis aries GN=CYB561 PE=2 SV=1	[more]
CY561_BOVIN	3.954e-21	37.35	Cytochrome b561 OS=Bos taurus GN=CYB561 PE=1 SV=2	[more]
CYBR1_HUMAN	1.150e-20	35.63	Cytochrome b reductase 1 OS=Homo sapiens GN=CYBRD1...	[more]
CY561_PONAB	1.962e-20	38.18	Cytochrome b561 OS=Pongo abelii GN=CYB561 PE=2 SV=...	[more]
CYAC3_HUMAN	2.563e-20	33.69	Cytochrome b ascorbate-dependent protein 3 OS=Homo...	[more]
CYBR1_XENTR	3.347e-20	35.63	Cytochrome b reductase 1 OS=Xenopus tropicalis GN=...	[more]
CY561_PIG	5.709e-20	36.75	Cytochrome b561 OS=Sus scrofa GN=CYB561 PE=2 SV=1	[more]
CY561_XENLA	9.738e-20	34.55	Cytochrome b561 OS=Xenopus laevis GN=cyb561 PE=2 S...	[more]

BLAST of FG538768 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G26100.1	9.087e-70	67.71	\| Symbols: \| Cytochrome b561/ferric reductase tra...	[more]
AT5G38630.1	1.012e-44	46.07	\| Symbols: ACYB-1, CYB-1 \| cytochrome B561-1 \| chr...	[more]
AT4G25570.1	7.023e-38	44.00	\| Symbols: ACYB-2 \| Cytochrome b561/ferric reducta...	[more]
AT1G14730.1	3.167e-22	35.63	\| Symbols: \| Cytochrome b561/ferric reductase tra...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004877	Cytochrome b561, eukaryote	PFAM	PF03188	Cytochrom_B561	coord: 9..141 score: 3.6
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	no description	coord: 9..135 score: 1.5
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROFILE	PS50939	CYTOCHROME_B561	coord: 1..177 score: 36
None	No IPR available	PANTHER	PTHR10106	CYTOCHROME B561	coord: 9..180 score: 9.6
None	No IPR available	SEG	seg	seg	coord: 57..66 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG538768 ID=FG538768; Name=FG538768; organism=Pisum sativum; type=EST; length=599bp
ACAACAAGATCTCATCTATTCAATTCTTCATCCTTTACTCATGGTCATTG
GGTTTATCCTCTTAAGTGGAGAAGCAATTTTGGTGCATAGATGGTTACCT
GGATCCAAAGGGTTAAAGAAATTAATGCATTTATGGCTTCAAGGATTGGC
TTTATGTTTTGGGATATTTGGAATTTGGACTAAGTTTCAGGGAAAGGATG
ATGGGATTTTGGCTAATTTCTATAGTTTGCATTCATGGATGGGTTTTACT
CGTGTATCCCTCTTTGGAGCTCAGTGGCTGATAGGGTTCTCGAAGTTTTG
GCACCGAGGAGAAGTGCGAAGAGTGAGGAAAATGATGTTACCTTGGCATG
TATTTGTTGGTCTATACACTTATGCATTGGCAGTAGCAACGGCTGAAACT
GGATTACTAGAAAAGTTAACATTCTTGCAAACACAGAGAAATGTACCCAA
ACACAACACAGAGTCCATGGTTGTAAATACTTTAGGACTTTCTCTTGCTC
TCTTTAGTGGCTTTGTTATTTTAGCTGCTGTTTCACCTAATAACTATCAA
TTTCTTCAAACCAAAATTTTGTATTCATCTTAATAAAACCTTCATTTTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR004877	Cyt_b561_dom
IPR006593	Cyt_b561/ferric_Rdtase_TM

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