AM162084

EST Overview


Name	AM162084
Unique Name	AM162084
Type	EST
Organism	Pisum sativum (pea)
Sequence length	329

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162084 vs. TrEMBL
Match: B9HY29_POPTR (Phosphoglycerate kinase OS=Populus trichocarpa GN=POPTRDRAFT_833719 PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 3.248e-41
Identity = 83/90 (92.22%), Postives = 87/90 (96.67%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI+VVKADD IGPEVEKLVASLP+GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  122 PTIKYLISNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 3.248e-41
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: Q9LD57_ARATH (Phosphoglycerate kinase OS=Arabidopsis thaliana GN=AT3G12780 PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 1.220e-40
Identity = 82/90 (91.11%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEV KADD IGPEVE LVASLP GGVLLLENVRFYKEEEKNDPE AK
Sbjct:  122 PTIKYLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFYKEEEKNDPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 1.220e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: B9RHY4_RICCO (Phosphoglycerate kinase OS=Ricinus communis GN=RCOM_1574580 PE=3 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.590e-40
Identity = 81/90 (90.00%), Postives = 87/90 (96.67%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PT+K+LI+NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI+VVKADD IGPEVEKLVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  120 PTVKHLIQNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 209

HSP 2 Score: 25.7942 bits (55), Expect = 1.590e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  216 DLYVNDAFGTA 226

[Close]

BLAST of AM162084 vs. TrEMBL
Match: O82160_POPNI (Phosphoglycerate kinase OS=Populus nigra GN=PnChlPGK PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.071e-40
Identity = 82/90 (91.11%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI  GAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI+VVKADD IGPEVEKLVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  122 PTIKYLISKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 2.071e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: D7L0K4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478641 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.071e-40
Identity = 82/90 (91.11%), Postives = 84/90 (93.33%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEV KADD IGPEVE LVASLP GGVLLLENVRFYKEEEKNDPE AK
Sbjct:  122 PTIKYLIDNGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFYKEEEKNDPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 2.071e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: B9HI53_POPTR (Phosphoglycerate kinase OS=Populus trichocarpa GN=POPTRDRAFT_832630 PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.071e-40
Identity = 82/90 (91.11%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI  GAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI+VVKADD IGPEVEKLVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  122 PTIKYLISKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 2.071e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: A9PIR4_9ROSI (Phosphoglycerate kinase OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 2.071e-40
Identity = 82/90 (91.11%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI  GAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI+VVKADD IGPEVEKLVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  122 PTIKYLISKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 2.071e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TrEMBL
Match: A5CAF8_VITVI (Phosphoglycerate kinase OS=Vitis vinifera GN=VITISV_000725 PE=3 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 3.517e-40
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI++VKADD IGPEVEKLV +LP+GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  119 PTIKYLIDNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQIVKADDCIGPEVEKLVTALPDGGVLLLENVRFYKEEEKNEPEFAK 208

HSP 2 Score: 25.7942 bits (55), Expect = 3.517e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  215 DLYVNDAFGTA 225

[Close]

BLAST of AM162084 vs. TrEMBL
Match: D7T0W2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_85.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036773001 PE=4 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 3.523e-40
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+GI++VKADD IGPEVEKLV +LP+GGVLLLENVRFYKEEEKN+PE AK
Sbjct:   46 PTIKYLIDNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQIVKADDCIGPEVEKLVTALPDGGVLLLENVRFYKEEEKNEPEFAK 135

HSP 2 Score: 25.7942 bits (55), Expect = 3.523e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  142 DLYVNDAFGTA 152

[Close]

BLAST of AM162084 vs. TrEMBL
Match: D7KNW9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474689 PE=4 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 7.778e-40
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+LI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEVVKADD IGP+VE LVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  119 PTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPQVESLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 208

HSP 2 Score: 25.7942 bits (55), Expect = 7.778e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  215 DLYVNDAFGTA 225

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_SPIOL (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea PE=2 SV=2)

HSP 1 Score: 162.54 bits (410), Expect = 2.902e-41
Identity = 80/90 (88.89%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+LI NGAKVILSSHLGRPKGVTPKFSLAPLVPRLSEL+G++VVKADD IGP+VEKLVA LP GGVLLLENVRFYKEEEKNDPE AK
Sbjct:   74 PTIKHLINNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLLENVRFYKEEEKNDPEFAK 163

HSP 2 Score: 25.7942 bits (55), Expect = 2.902e-41
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  170 DLYVNDAFGTA 180

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_ARATH (Phosphoglycerate kinase, chloroplastic OS=Arabidopsis thaliana GN=At1g56190 PE=1 SV=2)

HSP 1 Score: 161.77 bits (408), Expect = 4.921e-41
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+LI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEVVKADD IGPEVE LVASLP GGVLLLENVRFYKEEEKN+P+ AK
Sbjct:  119 PTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFYKEEEKNEPDFAK 208

HSP 2 Score: 25.7942 bits (55), Expect = 4.921e-41
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  215 DLYVNDAFGTA 225

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_TOBAC (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.086e-40
Identity = 78/90 (86.67%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+L+ NGAKVILSSHLGRPKGVTPK+SLAPLVPRLSEL+GI+VVK +D IGPEVEKLVASLP GGVLLLENVRFYKEEEKN+PE AK
Sbjct:  121 PTIKHLMANGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAK 210

HSP 2 Score: 25.7942 bits (55), Expect = 4.086e-40
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  217 DLYVNDAFGTA 227

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_WHEAT (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 1.534e-39
Identity = 79/90 (87.78%), Postives = 83/90 (92.22%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYL+ NGAKVIL+SHLGRPKGVTPKFSLAPLVPRLSEL+GIEV KA+D IGPEVEKLVA L NG VLLLENVRFYKEEEKNDPE AK
Sbjct:  118 PTIKYLLSNGAKVILTSHLGRPKGVTPKFSLAPLVPRLSELLGIEVKKAEDVIGPEVEKLVADLANGAVLLLENVRFYKEEEKNDPEFAK 207

HSP 2 Score: 24.2534 bits (51), Expect = 1.534e-39
Identity = 9/11 (81.82%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DL+VNDAFGT+
Sbjct:  214 DLFVNDAFGTA 224

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKY_WHEAT (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.047e-37
Identity = 74/90 (82.22%), Postives = 81/90 (90.00%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYL++ GAKVIL+SHLGRPKGVTPKFSL PLV RLSEL+G+EVV A D IG EVEKL A+LP+GGVLLLENVRFYKEEEKNDPE AK
Sbjct:   47 PTIKYLLEKGAKVILASHLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEKLAAALPDGGVLLLENVRFYKEEEKNDPEFAK 136

HSP 2 Score: 25.7942 bits (55), Expect = 3.047e-37
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  143 DLYVNDAFGTA 153

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKY_TOBAC (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.047e-37
Identity = 72/90 (80.00%), Postives = 83/90 (92.22%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYL+++G+ VIL+SHLGRPKGVTPK+SL PLVPRLSEL+G+EV  A+DSIGPEVEKLVA +P GGVLLLENVRFYKEEEKN+PE AK
Sbjct:   47 PTIKYLMQHGSHVILASHLGRPKGVTPKYSLKPLVPRLSELLGVEVKIANDSIGPEVEKLVAEIPEGGVLLLENVRFYKEEEKNEPEFAK 136

HSP 2 Score: 25.7942 bits (55), Expect = 3.047e-37
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  143 DLYVNDAFGTA 153

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_VOLCA (Phosphoglycerate kinase, chloroplastic OS=Volvox carteri GN=PGK PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.950e-30
Identity = 64/91 (70.33%), Postives = 74/91 (81.32%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PT+KYL+ NGAKV+L+SHLGRPKG    K+ L P+V RLSEL+G EV K DD IGP VE+ VASL +G +LLLENVRFYKEEEKNDPE AK
Sbjct:  107 PTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKEVKKVDDCIGPSVEQAVASLKSGELLLLENVRFYKEEEKNDPEFAK 197

HSP 2 Score: 25.7942 bits (55), Expect = 3.950e-30
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  204 DLYVNDAFGTA 214

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGKH_CHLRE (Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.262e-29
Identity = 61/91 (67.03%), Postives = 72/91 (79.12%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKG-VTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PT+KYL+ NGAKV+L+SHLGRPKG    K+ L P+V RLSEL+G  V K DD IGPEVEK V ++ NG +LLLEN RFYKEEEKN+PE AK
Sbjct:  106 PTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKPVTKVDDCIGPEVEKAVGAMKNGELLLLENCRFYKEEEKNEPEFAK 196

HSP 2 Score: 25.7942 bits (55), Expect = 3.262e-29
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  203 DLYVNDAFGTA 213

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGK_ANAVT (Phosphoglycerate kinase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=pgk PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 5.540e-29
Identity = 64/90 (71.11%), Postives = 70/90 (77.78%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTI+ L + GAKVIL+SH GRPKGV  K  L P+  RLSEL+G EVVK DDSIG EV   VA+L NG VLLLENVRFYKEEEKNDPE AK
Sbjct:   46 PTIQDLTQKGAKVILASHFGRPKGVDEKLRLTPVAKRLSELLGQEVVKTDDSIGDEVAAKVAALQNGQVLLLENVRFYKEEEKNDPEFAK 135

HSP 2 Score: 24.2534 bits (51), Expect = 5.540e-29
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            D YVNDAFGT+
Sbjct:  142 DFYVNDAFGTA 152

[Close]

BLAST of AM162084 vs. SwissProt
Match: PGK_ANASP (Phosphoglycerate kinase OS=Anabaena sp. (strain PCC 7120) GN=pgk PE=1 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 7.213e-29
Identity = 63/90 (70.00%), Postives = 70/90 (77.78%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTI+ L + GAKVIL+SH GRPKGV  K  L P+  RLSEL+G EV+K DDSIG EV   VA+L NG VLLLENVRFYKEEEKNDPE AK
Sbjct:   46 PTIQDLTQKGAKVILASHFGRPKGVDEKLRLTPVAKRLSELLGQEVIKTDDSIGDEVAAKVATLQNGQVLLLENVRFYKEEEKNDPEFAK 135

HSP 2 Score: 24.2534 bits (51), Expect = 7.213e-29
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            D YVNDAFGT+
Sbjct:  142 DFYVNDAFGTA 152

[Close]

BLAST of AM162084 vs. TAIR peptide
Match: AT3G12780.1 (| Symbols: PGK1 | phosphoglycerate kinase 1 | chr3:4061127-4063140 REVERSE LENGTH=481)

HSP 1 Score: 164.851 bits (416), Expect = 4.696e-43
Identity = 82/90 (91.11%), Postives = 85/90 (94.44%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYLI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEV KADD IGPEVE LVASLP GGVLLLENVRFYKEEEKNDPE AK
Sbjct:  122 PTIKYLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFYKEEEKNDPEFAK 211

HSP 2 Score: 25.7942 bits (55), Expect = 4.696e-43
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  218 DLYVNDAFGTA 228

[Close]

BLAST of AM162084 vs. TAIR peptide
Match: AT1G56190.1 (| Symbols: | Phosphoglycerate kinase family protein | chr1:21028403-21030454 FORWARD LENGTH=478)

HSP 1 Score: 161.77 bits (408), Expect = 3.901e-42
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+LI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEVVKADD IGPEVE LVASLP GGVLLLENVRFYKEEEKN+P+ AK
Sbjct:  119 PTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFYKEEEKNEPDFAK 208

HSP 2 Score: 25.7942 bits (55), Expect = 3.901e-42
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  215 DLYVNDAFGTA 225

[Close]

BLAST of AM162084 vs. TAIR peptide
Match: AT1G56190.2 (| Symbols: | Phosphoglycerate kinase family protein | chr1:21028622-21030454 FORWARD LENGTH=405)

HSP 1 Score: 161.77 bits (408), Expect = 3.908e-42
Identity = 80/90 (88.89%), Postives = 86/90 (95.56%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIK+LI+NGAKVILS+HLGRPKGVTPKFSLAPLVPRLSEL+GIEVVKADD IGPEVE LVASLP GGVLLLENVRFYKEEEKN+P+ AK
Sbjct:   46 PTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFYKEEEKNEPDFAK 135

HSP 2 Score: 25.7942 bits (55), Expect = 3.908e-42
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            DLYVNDAFGT+
Sbjct:  142 DLYVNDAFGTA 152

[Close]

BLAST of AM162084 vs. TAIR peptide
Match: AT1G79550.2 (| Symbols: PGK | phosphoglycerate kinase | chr1:29924347-29926295 REVERSE LENGTH=401)

HSP 1 Score: 148.673 bits (374), Expect = 6.957e-38
Identity = 72/90 (80.00%), Postives = 81/90 (90.00%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYL+ NG++V+L SHLGRPKGVTPK+SL PLVPRLSEL+G+EVV A+DSIG EV+KLVA LP GGVLLLENVRFY EEEKNDPE AK
Sbjct:   47 PTIKYLMGNGSRVVLCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFYAEEEKNDPEFAK 136

HSP 2 Score: 24.6386 bits (52), Expect = 6.957e-38
Identity = 9/11 (81.82%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            D+YVNDAFGT+
Sbjct:  143 DVYVNDAFGTA 153

[Close]

BLAST of AM162084 vs. TAIR peptide
Match: AT1G79550.1 (| Symbols: PGK | phosphoglycerate kinase | chr1:29924347-29926295 REVERSE LENGTH=401)

HSP 1 Score: 148.673 bits (374), Expect = 6.957e-38
Identity = 72/90 (80.00%), Postives = 81/90 (90.00%), Query Frame = 2

Query:    2 PTIKYLIKNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELIGIEVVKADDSIGPEVEKLVASLPNGGVLLLENVRFYKEEEKNDPEHAK 271
            PTIKYL+ NG++V+L SHLGRPKGVTPK+SL PLVPRLSEL+G+EVV A+DSIG EV+KLVA LP GGVLLLENVRFY EEEKNDPE AK
Sbjct:   47 PTIKYLMGNGSRVVLCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFYAEEEKNDPEFAK 136

HSP 2 Score: 24.6386 bits (52), Expect = 6.957e-38
Identity = 9/11 (81.82%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  289 DLYVNDAFGTS 321
            D+YVNDAFGT+
Sbjct:  143 DVYVNDAFGTA 153

The following BLAST results are available for this feature:

BLAST of AM162084 vs. TrEMBL
BLAST of AM162084 vs. SwissProt
BLAST of AM162084 vs. TAIR peptide

BLAST of AM162084 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HY29_POPTR	3.248e-41	92.22	Phosphoglycerate kinase OS=Populus trichocarpa GN=...	[more]
Q9LD57_ARATH	1.220e-40	91.11	Phosphoglycerate kinase OS=Arabidopsis thaliana GN...	[more]
B9RHY4_RICCO	1.590e-40	90.00	Phosphoglycerate kinase OS=Ricinus communis GN=RCO...	[more]
O82160_POPNI	2.071e-40	91.11	Phosphoglycerate kinase OS=Populus nigra GN=PnChlP...	[more]
D7L0K4_ARALY	2.071e-40	91.11	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9HI53_POPTR	2.071e-40	91.11	Phosphoglycerate kinase OS=Populus trichocarpa GN=...	[more]
A9PIR4_9ROSI	2.071e-40	91.11	Phosphoglycerate kinase OS=Populus trichocarpa x P...	[more]
A5CAF8_VITVI	3.517e-40	88.89	Phosphoglycerate kinase OS=Vitis vinifera GN=VITIS...	[more]
D7T0W2_VITVI	3.523e-40	88.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KNW9_ARALY	7.778e-40	88.89	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of AM162084 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PGKH_SPIOL	2.902e-41	88.89	Phosphoglycerate kinase, chloroplastic (Fragment) ...	[more]
PGKH_ARATH	4.921e-41	88.89	Phosphoglycerate kinase, chloroplastic OS=Arabidop...	[more]
PGKH_TOBAC	4.086e-40	86.67	Phosphoglycerate kinase, chloroplastic OS=Nicotian...	[more]
PGKH_WHEAT	1.534e-39	87.78	Phosphoglycerate kinase, chloroplastic OS=Triticum...	[more]
PGKY_WHEAT	3.047e-37	82.22	Phosphoglycerate kinase, cytosolic OS=Triticum aes...	[more]
PGKY_TOBAC	3.047e-37	80.00	Phosphoglycerate kinase, cytosolic OS=Nicotiana ta...	[more]
PGKH_VOLCA	3.950e-30	70.33	Phosphoglycerate kinase, chloroplastic OS=Volvox c...	[more]
PGKH_CHLRE	3.262e-29	67.03	Phosphoglycerate kinase, chloroplastic OS=Chlamydo...	[more]
PGK_ANAVT	5.540e-29	71.11	Phosphoglycerate kinase OS=Anabaena variabilis (st...	[more]
PGK_ANASP	7.213e-29	70.00	Phosphoglycerate kinase OS=Anabaena sp. (strain PC...	[more]

BLAST of AM162084 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G12780.1	4.696e-43	91.11	\| Symbols: PGK1 \| phosphoglycerate kinase 1 \| chr3...	[more]
AT1G56190.1	3.901e-42	88.89	\| Symbols: \| Phosphoglycerate kinase family prote...	[more]
AT1G56190.2	3.908e-42	88.89	\| Symbols: \| Phosphoglycerate kinase family prote...	[more]
AT1G79550.2	6.957e-38	80.00	\| Symbols: PGK \| phosphoglycerate kinase \| chr1:29...	[more]
AT1G79550.1	6.957e-38	80.00	\| Symbols: PGK \| phosphoglycerate kinase \| chr1:29...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001576	Phosphoglycerate kinase	PANTHER	PTHR11406	PHOSPHOGLYCERATE KINASE	coord: 1..84 score: 1.6
IPR001576	Phosphoglycerate kinase	PFAM	PF00162	PGK	coord: 1..88 score: 9.8
IPR001576	Phosphoglycerate kinase	SUPERFAMILY	53748	Phosphoglycerate kinase	coord: 1..91 score: 1.5
IPR015824	Phosphoglycerate kinase, N-terminal	GENE3D	3.40.50.1260	no description	coord: 1..91 score: 7
None	No IPR available	SEG	seg	seg	coord: 38..46 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162084 ID=AM162084; Name=AM162084; organism=Pisum sativum; type=EST; length=329bp
ACCAACTATCAAATATCTTATTAAGAATGGCGCTAAAGTCATCCTTTCCA
GTCACTTGGGACGGCCAAAGGGTGTCACTCCCAAATTCAGCTTGGCACCT
CTTGTTCCCCGGCTATCCGAACTCATCGGAATCGAGGTTGTTAAGGCCGA
TGACAGTATTGGTCCAGAAGTAGAAAAGTTGGTGGCTTCCCTTCCAAACG
GAGGAGTTCTTCTTCTAGAAAATGTAAGGTTTTACAAGGAGGAAGAAAAG
AACGATCCTGAGCATGCAAAAAGCTCGCTGCACTAGCAGATCTGTATGTG
AATGATGCTTTTGGTACCTCGGCCGCGAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004618	phosphoglycerate kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001576	Phosphoglycerate_kinase
IPR015824	Phosphoglycerate_kinase_N

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