AM162105

EST Overview


Name	AM162105
Unique Name	AM162105
Type	EST
Organism	Pisum sativum (pea)
Sequence length	462

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162105 vs. TrEMBL
Match: O65027_SOYBN (Aspartokinase-homoserine dehydrogenase (Fragment) OS=Glycine max GN=AK-HSDH PE=4 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 3.228e-68
Identity = 124/153 (81.05%), Postives = 140/153 (91.50%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED IKALRAVHSRFY S TTIAMGIIGPGLIGSTL+DQLRDQA+TLKEE NIDLRVMGI+GSKSMLL D GIDL +W E+REE+GEV ++EKFVQHVHGNHFIPNT LVDCTADS +A +Y DWLRKGIHV+TPNKKANSGPL++
Sbjct:  530 NITVVVKREDCIKALRAVHSRFYLSRTTIAMGIIGPGLIGSTLLDQLRDQASTLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWRELREERGEVANVEKFVQHVHGNHFIPNTALVDCTADSAIAGYYYDWLRKGIHVVTPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. TrEMBL
Match: O63067_SOYBN (Aspartokinase-homoserine dehydrogenase OS=Glycine max GN=AK-HSDH PE=2 SV=1)

HSP 1 Score: 258.455 bits (659), Expect = 1.602e-67
Identity = 123/153 (80.39%), Postives = 140/153 (91.50%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED IKALRAVHSRFY S TTIAMGIIGPGLIGSTL++QLRDQA+TLKEE NIDLRVMGI+GSKSMLL D GIDL +W E+REE+GEV ++EKFVQHVHGNHFIPNT LVDCTADS +A +Y DWLRKGIHV+TPNKKANSGPL++
Sbjct:  530 NITVVVKREDCIKALRAVHSRFYLSRTTIAMGIIGPGLIGSTLLEQLRDQASTLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWRELREERGEVANMEKFVQHVHGNHFIPNTALVDCTADSVIAGYYYDWLRKGIHVVTPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. TrEMBL
Match: D7TL78_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_82.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036290001 PE=4 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.958e-65
Identity = 120/153 (78.43%), Postives = 137/153 (89.54%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+AL+AVHSRFY S TTIAMGIIGPGLIG TL+DQLRDQAA LKE+ NIDLRVMGI GS++MLL DSGIDL +W E+ +EKGEV D+ KFV HVHGNHFIPNT LVDCTADS VASHY++WLRKGIHVITPNKKANSGPL++
Sbjct:  485 NITVVVKREDCIRALKAVHSRFYLSRTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLSDSGIDLSRWRELIKEKGEVGDMHKFVNHVHGNHFIPNTALVDCTADSNVASHYHEWLRKGIHVITPNKKANSGPLDQ 637

[Close]

BLAST of AM162105 vs. TrEMBL
Match: A5APD8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034964 PE=4 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.958e-65
Identity = 120/153 (78.43%), Postives = 137/153 (89.54%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+AL+AVHSRFY S TTIAMGIIGPGLIG TL+DQLRDQAA LKE+ NIDLRVMGI GS++MLL DSGIDL +W E+ +EKGEV D+ KFV HVHGNHFIPNT LVDCTADS VASHY++WLRKGIHVITPNKKANSGPL++
Sbjct:  455 NITVVVKREDCIRALKAVHSRFYLSRTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLSDSGIDLSRWRELIKEKGEVGDMHKFVNHVHGNHFIPNTALVDCTADSNVASHYHEWLRKGIHVITPNKKANSGPLDQ 607

[Close]

BLAST of AM162105 vs. TrEMBL
Match: B9MWJ3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_288174 PE=4 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 3.693e-64
Identity = 119/153 (77.78%), Postives = 138/153 (90.20%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KR D I+ALRAVHSRFY S TTIAMGIIGPGLIG+TL+DQLRDQAA LKE+ NIDLRVMGI GS++MLL+D GIDL +W E+ ++KGEV DLEKF QHVHGNHF+PNTVLVDCTADS VAS Y+DWLR+GIHVITPNKKANSGPL++
Sbjct:  457 NITVVIKRGDCIRALRAVHSRFYLSKTTIAMGIIGPGLIGATLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLNDVGIDLSRWRELVKDKGEVADLEKFRQHVHGNHFLPNTVLVDCTADSNVASCYHDWLRRGIHVITPNKKANSGPLDQ 609

[Close]

BLAST of AM162105 vs. TrEMBL
Match: B9IQ92_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_780515 PE=4 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 8.228e-64
Identity = 120/153 (78.43%), Postives = 137/153 (89.54%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+ALRAVHSRFY S TTIAMGIIGPGLIG+TL+DQLRDQAA LKE+ NIDLRVMGI GS++MLL D  IDL KW E+ ++KGEV DLEKF QHVHGN+FIPNTVLVDCTADS VAS Y+DWLR+GIHVITPNKKANSGPL++
Sbjct:  443 NITVVIKREDCIRALRAVHSRFYLSKTTIAMGIIGPGLIGATLLDQLRDQAAFLKEDFNIDLRVMGITGSRTMLLSDVEIDLSKWRELVKDKGEVADLEKFTQHVHGNNFIPNTVLVDCTADSSVASCYHDWLRRGIHVITPNKKANSGPLDQ 595

[Close]

BLAST of AM162105 vs. TrEMBL
Match: B9SHP2_RICCO (Aspartate kinase, putative OS=Ricinus communis GN=RCOM_0742100 PE=4 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.833e-63
Identity = 119/153 (77.78%), Postives = 131/153 (85.62%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED IKALRAVHSRFY S TTIAMGIIGPGLIG TL+DQLRDQAA LKEE NIDLRVMGI GS+ MLL + GIDL +W E+  E GEV D+EKF  HVHGNHFIPNTVLVDCTAD+ VA  Y DWLRKGIHVITPNKKANSGPL++
Sbjct:  534 NITVVVKREDCIKALRAVHSRFYLSKTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEEFNIDLRVMGITGSRRMLLSEVGIDLSRWRELTRENGEVADMEKFTHHVHGNHFIPNTVLVDCTADTSVAKCYYDWLRKGIHVITPNKKANSGPLDQ 686

[Close]

BLAST of AM162105 vs. TrEMBL
Match: D7MG88_ARALY (AK-HSDH/AK-HSDH II OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492891 PE=4 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 4.084e-63
Identity = 116/153 (75.82%), Postives = 135/153 (88.24%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            NV+VV+KREDS+KALRAVHSRF+ S TT+AMGIIGPGLIG+TL+DQLRDQAA LK++ NIDLRVMGI GSK+MLL D GIDL +W E+  EKG   +++KF Q VHGNHFIPN+VLVDCTADS +ASHY DWLRKGIHVITPNKKANSGPL++
Sbjct:  535 NVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIIGPGLIGATLLDQLRDQAAVLKQKFNIDLRVMGITGSKTMLLSDIGIDLSRWRELLNEKGTEANMDKFTQQVHGNHFIPNSVLVDCTADSAIASHYYDWLRKGIHVITPNKKANSGPLDQ 687

[Close]

BLAST of AM162105 vs. TrEMBL
Match: Q0WRP9_ARATH (Aspartate kinase-homoserine dehydrogenase-like protein OS=Arabidopsis thaliana GN=At4g19710 PE=2 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 1.552e-62
Identity = 115/153 (75.16%), Postives = 133/153 (86.93%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            NV+VV+KREDS+KALRAVHSRF+ S TT+AMGI+GPGLIG+TL+DQLRDQAA LK+E NIDLRV+GI GSK MLL D GIDL +W E+  EKG   DL+KF Q VHGNHFIPN+V+VDCTADS +AS Y DWLRKGIHVITPNKKANSGPL++
Sbjct:  530 NVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. TrEMBL
Match: D7KK95_ARALY (AK-HSDH I OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473611 PE=4 SV=1)

HSP 1 Score: 223.402 bits (568), Expect = 5.711e-57
Identity = 104/153 (67.97%), Postives = 131/153 (85.62%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+ALRAVHSRFY S TT+A+GIIGPGLIG TL+DQ+RDQAA LKEE  IDLRV+GI GS  ML+ +SGIDL +W ++ +E+GE  ++EKF Q+V GNHFIPN+V+VDCTAD+ +AS Y DWL +GIHV+TPNKKANSGPL++
Sbjct:  525 NITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEYKIDLRVIGITGSSKMLMSESGIDLSRWRKLMKEEGEKANMEKFTQYVKGNHFIPNSVMVDCTADADIASCYYDWLLRGIHVVTPNKKANSGPLDQ 677

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH2_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH2 PE=1 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 9.959e-64
Identity = 115/153 (75.16%), Postives = 133/153 (86.93%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            NV+VV+KREDS+KALRAVHSRF+ S TT+AMGI+GPGLIG+TL+DQLRDQAA LK+E NIDLRV+GI GSK MLL D GIDL +W E+  EKG   DL+KF Q VHGNHFIPN+V+VDCTADS +AS Y DWLRKGIHVITPNKKANSGPL++
Sbjct:  530 NVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH_DAUCA (Bifunctional aspartokinase/homoserine dehydrogenase, chloroplastic (Fragment) OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 6.455e-63
Identity = 112/153 (73.20%), Postives = 136/153 (88.89%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VVL RED ++AL+AVHSRFY S TTIA+GI+GPGLIG+TL+DQLRDQAA LKE S IDLRVMGI GS++MLL ++GIDL +W E+++EKG+   LEKFVQHV GNHFIP+TV+VDCTADS VASHY+DWL +GIHVITPNKKANSGPL++
Sbjct:  535 NITVVLSREDCVRALKAVHSRFYLSRTTIAVGIVGPGLIGATLLDQLRDQAAILKENSKIDLRVMGITGSRTMLLSETGIDLSRWREVQKEKGQTAGLEKFVQHVRGNHFIPSTVIVDCTADSEVASHYHDWLCRGIHVITPNKKANSGPLDQ 687

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH1_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH1 PE=1 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 3.315e-59
Identity = 106/153 (69.28%), Postives = 131/153 (85.62%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+ALRAVHSRFY S TT+A+GIIGPGLIG TL+DQ+RDQAA LKEE  IDLRV+GI GS  ML+ +SGIDL +W E+ +E+GE  D+EKF Q+V GNHFIPN+V+VDCTAD+ +AS Y DWL +GIHV+TPNKKANSGPL++
Sbjct:  525 NITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEFKIDLRVIGITGSSKMLMSESGIDLSRWRELMKEEGEKADMEKFTQYVKGNHFIPNSVMVDCTADADIASCYYDWLLRGIHVVTPNKKANSGPLDQ 677

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH1_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Zea mays GN=AKHSDH1 PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 1.179e-56
Identity = 102/152 (67.11%), Postives = 126/152 (82.89%), Query Frame = -3

Query:    5 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLN 460
            N+++VLK+ED ++ALRA HSRF+ S TT+A+GIIGPGLIG TL++QL+DQAA LKE  NIDLRVMGI GS++MLL D G+DL +W E  + + E  +L+KFV H+  NHF PN VLVDCTAD+ VASHY DWL+KGIHVITPNKKANSGPL+
Sbjct:  534 NITIVLKQEDCVRALRAAHSRFFLSKTTLAVGIIGPGLIGRTLLNQLKDQAAVLKENMNIDLRVMGIAGSRTMLLSDIGVDLTQWKEKLQTEAEPANLDKFVHHLSENHFFPNRVLVDCTADTSVASHYYDWLKKGIHVITPNKKANSGPLD 685

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH2_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Zea mays GN=AKHSDH2 PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 5.477e-54
Identity = 96/153 (62.75%), Postives = 124/153 (81.05%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VVLK++D ++ALRA HSRF+ S TT+A+GIIGPGLIG  L++QL++Q A LKE  NIDLRV+GI GS +MLL D+GIDL +W ++ +++ E  D+  FV H+  NH  PN VLVDCTAD+ VASHY DWL+KGIHVITPNKKANSGPL++
Sbjct:  531 NITVVLKQQDCVRALRAAHSRFFLSKTTLAVGIIGPGLIGGALLNQLKNQTAVLKENMNIDLRVIGITGSSTMLLSDTGIDLTQWKQLLQKEAEPADIGSFVHHLSDNHVFPNKVLVDCTADTSVASHYYDWLKKGIHVITPNKKANSGPLDQ 683

[Close]

BLAST of AM162105 vs. SwissProt
Match: AK1H_ECOLI (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Escherichia coli (strain K12) GN=thrA PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 4.090e-25
Identity = 57/152 (37.50%), Postives = 95/152 (62.50%), Query Frame = -3

Query:    5 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLN 460
            ++SVV+  +D+   +R  H   +N++  I + +IG G +G  L++QL+ Q + LK + +IDLRV G+  SK++L +  G++L  W E   +  E  +L + ++ V   H + N V+VDCT+   VA  Y D+LR+G HV+TPNKKAN+  ++
Sbjct:  438 SISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNK-HIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLL-NPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMD 587

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH_BUCBP (Bifunctional aspartokinase/homoserine dehydrogenase OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) GN=thrA PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.482e-22
Identity = 50/148 (33.78%), Postives = 89/148 (60.14%), Query Frame = -3

Query:   17 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANS 460
            ++S+V+K +D I  +RA+H   +N N T  + +IG G +G T + Q+ +Q   LK + NIDL++ GI  SK+ +L+  GI+   W        +  + +  +  +  N+++ N ++VDCT+D  +A+ Y  ++  G H++TPNKKAN+
Sbjct:  439 SISIVVKHDDGILGVRALHKGIFNKNCTAEIFLIGIGRVGQTFLKQIIEQKNWLKSK-NIDLKICGIANSKNFILNLDGINPKNWKRDLNLSKKSFNFKHLLNSIQ-NYYLINPIIVDCTSDKNIANQYISFINCGFHIVTPNKKANT 584

[Close]

BLAST of AM162105 vs. SwissProt
Match: AK1H_SERMA (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Serratia marcescens GN=thrA PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 4.233e-22
Identity = 57/152 (37.50%), Postives = 88/152 (57.89%), Query Frame = -3

Query:    5 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLN 460
            ++SVV+  + +   +R  H   +N++  I + +IG G +G  LI+Q+  Q   LK++ +IDLRV GI  S+ ML +  GI L  W +      E  +L + ++ V   H + N V+VDCT+   VA  Y D+L  G HV+TPNKKAN+  +N
Sbjct:  438 SISVVVSNDSATTGVRVSHQMLFNTDQVIEVFVIGVGGVGGALIEQIYRQQPWLKQK-HIDLRVCGIANSRVMLTNVHGIALDSWRDALAGAQEPFNLGRLIRLVKEYHLL-NPVIVDCTSSQAVADQYVDFLADGFHVVTPNKKANTSSMN 587

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH_HAEIN (Bifunctional aspartokinase/homoserine dehydrogenase OS=Haemophilus influenzae GN=thrA PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.232e-21
Identity = 52/151 (34.44%), Postives = 90/151 (59.60%), Query Frame = -3

Query:    8 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPL 460
            ++S V+ +  +I+A++A H   +N+   + M ++G G +G  LI+Q++ Q   L ++ N+++RV  I  S  MLLD++G++L  W    E   + +D +  +  +  +H + N V VDCT+   VA  Y   L++G HV+TPNKKAN+  L
Sbjct:  438 SISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGELIEQVKRQKEYLAKK-NVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVV-NPVFVDCTSAESVAGLYARALKEGFHVVTPNKKANTREL 586

[Close]

BLAST of AM162105 vs. SwissProt
Match: AKH_BUCAI (Bifunctional aspartokinase/homoserine dehydrogenase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=thrA PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.043e-20
Identity = 54/152 (35.53%), Postives = 93/152 (61.18%), Query Frame = -3

Query:    5 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLN 460
            ++S+V+K+E+ +KA++ VH+  + +  TI M +IG G IGSTL++Q+  Q   L ++ NI++++  I  SK +LL D+  DL  W    ++  +  +LE     +  NHF  N+V+VDCT+   ++  Y +++    HV+T NKKAN+   N
Sbjct:  439 SISLVIKKENILKAVQHVHNTLFFNKKTIHMFLIGIGGIGSTLLNQILKQKQFL-DKKNIEIKICAIANSKKILLLDNTNDLSNWKNDFKKSTQKFNLELLNNLIKNNHF-SNSVIVDCTSSQLLSEQYVNFIDNNFHVVTSNKKANTSEWN 588

[Close]

BLAST of AM162105 vs. TAIR peptide
Match: AT4G19710.2 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729536 FORWARD LENGTH=916)

HSP 1 Score: 241.891 bits (616), Expect = 1.161e-64
Identity = 115/153 (75.16%), Postives = 133/153 (86.93%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            NV+VV+KREDS+KALRAVHSRF+ S TT+AMGI+GPGLIG+TL+DQLRDQAA LK+E NIDLRV+GI GSK MLL D GIDL +W E+  EKG   DL+KF Q VHGNHFIPN+V+VDCTADS +AS Y DWLRKGIHVITPNKKANSGPL++
Sbjct:  530 NVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. TAIR peptide
Match: AT4G19710.1 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729278 FORWARD LENGTH=859)

HSP 1 Score: 241.891 bits (616), Expect = 1.161e-64
Identity = 115/153 (75.16%), Postives = 133/153 (86.93%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            NV+VV+KREDS+KALRAVHSRF+ S TT+AMGI+GPGLIG+TL+DQLRDQAA LK+E NIDLRV+GI GSK MLL D GIDL +W E+  EKG   DL+KF Q VHGNHFIPN+V+VDCTADS +AS Y DWLRKGIHVITPNKKANSGPL++
Sbjct:  530 NVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWRELLNEKGTEADLDKFTQQVHGNHFIPNSVVVDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQ 682

[Close]

BLAST of AM162105 vs. TAIR peptide
Match: AT1G31230.1 (| Symbols: AK-HSDH I, AK-HSDH | aspartate kinase-homoserine dehydrogenase i | chr1:11158744-11163055 REVERSE LENGTH=911)

HSP 1 Score: 226.868 bits (577), Expect = 3.865e-60
Identity = 106/153 (69.28%), Postives = 131/153 (85.62%), Query Frame = -3

Query:    2 NVSVVLKREDSIKALRAVHSRFYNSNTTIAMGIIGPGLIGSTLIDQLRDQAATLKEESNIDLRVMGIMGSKSMLLDDSGIDLVKWSEIREEKGEVTDLEKFVQHVHGNHFIPNTVLVDCTADSGVASHYNDWLRKGIHVITPNKKANSGPLNE 460
            N++VV+KRED I+ALRAVHSRFY S TT+A+GIIGPGLIG TL+DQ+RDQAA LKEE  IDLRV+GI GS  ML+ +SGIDL +W E+ +E+GE  D+EKF Q+V GNHFIPN+V+VDCTAD+ +AS Y DWL +GIHV+TPNKKANSGPL++
Sbjct:  525 NITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEFKIDLRVIGITGSSKMLMSESGIDLSRWRELMKEEGEKADMEKFTQYVKGNHFIPNSVMVDCTADADIASCYYDWLLRGIHVVTPNKKANSGPLDQ 677

The following BLAST results are available for this feature:

BLAST of AM162105 vs. TrEMBL
BLAST of AM162105 vs. SwissProt
BLAST of AM162105 vs. TAIR peptide

BLAST of AM162105 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O65027_SOYBN	3.228e-68	81.05	Aspartokinase-homoserine dehydrogenase (Fragment) ...	[more]
O63067_SOYBN	1.602e-67	80.39	Aspartokinase-homoserine dehydrogenase OS=Glycine ...	[more]
D7TL78_VITVI	1.958e-65	78.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5APD8_VITVI	1.958e-65	78.43	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9MWJ3_POPTR	3.693e-64	77.78	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9IQ92_POPTR	8.228e-64	78.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SHP2_RICCO	1.833e-63	77.78	Aspartate kinase, putative OS=Ricinus communis GN=...	[more]
D7MG88_ARALY	4.084e-63	75.82	AK-HSDH/AK-HSDH II OS=Arabidopsis lyrata subsp. ly...	[more]
Q0WRP9_ARATH	1.552e-62	75.16	Aspartate kinase-homoserine dehydrogenase-like pro...	[more]
D7KK95_ARALY	5.711e-57	67.97	AK-HSDH I OS=Arabidopsis lyrata subsp. lyrata GN=A...	[more]

BLAST of AM162105 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AKH2_ARATH	9.959e-64	75.16	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_DAUCA	6.455e-63	73.20	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH1_ARATH	3.315e-59	69.28	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH1_MAIZE	1.179e-56	67.11	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH2_MAIZE	5.477e-54	62.75	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AK1H_ECOLI	4.090e-25	37.50	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_BUCBP	2.482e-22	33.78	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AK1H_SERMA	4.233e-22	37.50	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_HAEIN	1.232e-21	34.44	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_BUCAI	1.043e-20	35.53	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]

BLAST of AM162105 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G19710.2	1.161e-64	75.16	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]
AT4G19710.1	1.161e-64	75.16	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]
AT1G31230.1	3.865e-60	69.28	\| Symbols: AK-HSDH I, AK-HSDH \| aspartate kinase-h...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005106	Aspartate/homoserine dehydrogenase, NAD-binding	PFAM	PF03447	NAD_binding_3	coord: 35..148 score: 8.8
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 27..152 score: 8.9
None	No IPR available	PANTHER	PTHR21499	ASPARTATE KINASE	coord: 1..153 score: 2
None	No IPR available	PANTHER	PTHR21499:SF1	ASPARTATE KINASE	coord: 1..153 score: 2
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 25..153 score: 2.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162105 ID=AM162105; Name=AM162105; organism=Pisum sativum; type=EST; length=462bp
ACTCATTAAGTGGTCCCGAATTTGCCTTTTTATTGGGAGTGATCACATGT
ATCCCTTTGCGCAGCCAGTCATTGTAATGACTTGCTACGCCAGAATCAGC
CGTGCAGTCTACTAAAACTGTGTTTGGTATAAAATGATTTCCATGCACAT
GTTGAACAAATTTTTCCAAATCGGTCACTTCGCCTTTTTCCTCTCGAATT
TCGCTCCACTTTACTAAGTCGATCCCCGAATCATCAAGGAGCATCGACTT
TGAACCCATGATGCCCATTACACGTAAATCAATGTTTGATTCTTCTTTCA
AAGTTGCTGCCTGATCCCTTAGCTGATCAATGAGTGTGCTCCCAATTAAC
CCAGGTCCAATTATGCCCATTGCTATGGTGGTGTTTGAGTTATAAAATCT
AGAATGGACAGCCCGAAGAGCCTTTATAGAATCCTCCCGCTTAAGAACAA
CACTAACATTGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0050661	NADP binding
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR005106	Asp/hSer_DH_NAD-bd
IPR016040	NAD(P)-bd_dom

Search form