EX570110

EST Overview
NameEX570110
Unique NameEX570110
TypeEST
OrganismPisum sativum (pea)
Sequence length268
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of EX570110 vs. TrEMBL
Match: Q9LYS8_ARATH (AT3g59080/F17J16_130 OS=Arabidopsis thaliana GN=F17J16_130 PE=2 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.376e-26
Identity = 59/89 (66.29%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LLSHPNLNFTSFV G+EN VDTFYYV IKS++V GE L+IPEETW +S +              YFA
Sbjct:  332 CLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 420
[Close]
BLAST of EX570110 vs. TrEMBL
Match: Q2V3N3_ARATH (Putative uncharacterized protein At3g59080.2 OS=Arabidopsis thaliana GN=At3g59080 PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.376e-26
Identity = 59/89 (66.29%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LLSHPNLNFTSFV G+EN VDTFYYV IKS++V GE L+IPEETW +S +              YFA
Sbjct:  296 CLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 384
[Close]
BLAST of EX570110 vs. TrEMBL
Match: D7LJF0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_903676 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 8.921e-26
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LL+H NLNFTSFV G+ENSV+TFYY+ IKS++V GEAL IPEETW +S +              YFA
Sbjct:  324 CLVDRNSDTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVGGEALDIPEETWNISPDGAGGTIIDSGTTLSYFA 412
[Close]
BLAST of EX570110 vs. TrEMBL
Match: D7LWA4_ARALY (Putative uncharacterized protein (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486375 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.522e-25
Identity = 58/89 (65.17%), Postives = 67/89 (75.28%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LLSHPNLNFTSFV  +EN VDTFYYV IKS++V GE L+IPEETW +S +              YFA
Sbjct:  317 CLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVARKENLVDTFYYVQIKSIIVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 405
[Close]
BLAST of EX570110 vs. TrEMBL
Match: B9S302_RICCO (Basic 7S globulin 2 small subunit, putative OS=Ricinus communis GN=RCOM_1192560 PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.987e-25
Identity = 58/89 (65.17%), Postives = 65/89 (73.03%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LL+HP +NFTS V G+EN VDTFYYV IKS+MV GE L IPEETW LS E              YFA
Sbjct:  354 CLVDRNSDTNVSSKLIFGEDKDLLNHPEVNFTSLVAGKENPVDTFYYVQIKSIMVGGEVLKIPEETWHLSPEGAGGTIVDSGTTLSYFA 442
[Close]
BLAST of EX570110 vs. TrEMBL
Match: Q9SJG1_ARATH (Putative chloroplast nucleoid DNA binding protein OS=Arabidopsis thaliana GN=At2g42980 PE=2 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 7.552e-25
Identity = 55/89 (61.80%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNS+++VSSKLIFGEDK+LL+H NLNFTSFV G+ENSV+TFYY+ IKS++V G+AL IPEETW +S +              YFA
Sbjct:  322 CLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFA 410
[Close]
BLAST of EX570110 vs. TrEMBL
Match: B9H837_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_205286 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.682e-24
Identity = 52/72 (72.22%), Postives = 63/72 (87.50%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEE 217
            CLVDRNSD++VSSKLIFGEDK+LL+HP LNFT+ VGG+EN VDTFYYV IKS+MV GE L+IPE TW ++ +
Sbjct:  252 CLVDRNSDTNVSSKLIFGEDKDLLNHPELNFTTLVGGKENPVDTFYYVQIKSIMVGGEVLNIPESTWNMTSD 323
[Close]
BLAST of EX570110 vs. TrEMBL
Match: Q5MJW8_TOBAC (Avr9/Cf-9 rapidly elicited protein 36 (Fragment) OS=Nicotiana tabacum GN=ACRE36 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.802e-18
Identity = 48/76 (63.16%), Postives = 53/76 (69.74%), Query Frame = 2 
Query:   41 KLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            KLIFGEDKELL H NLNFTS VGG+EN ++TFYYV IKSV+V GE L+IPEETW LS E              YFA
Sbjct:    1 KLIFGEDKELLKHLNLNFTSLVGGKENHLETFYYVQIKSVIVGGEVLNIPEETWNLSTEGVGGTIIDSGTTLSYFA 76
[Close]
BLAST of EX570110 vs. TrEMBL
Match: Q6Z6Q8_ORYSJ (Os02g0314600 protein OS=Oryza sativa subsp. japonica GN=P0705A04.34 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.929e-12
Identity = 38/89 (42.70%), Postives = 50/89 (56.18%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVD  S  SV SK++FG+D  LL HP LN+T+F      + DTFYYV +K V+V GE L+I   TW + ++              YFA
Sbjct:  312 CLVDHGS--SVGSKIVFGDDDALLGHPRLNYTAFAPSAAAAADTFYYVQLKGVLVGGEKLNISPSTWDVGKDGSGGTIIDSGTTLSYFA 398
[Close]
BLAST of EX570110 vs. TrEMBL
Match: A2X403_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_06935 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.929e-12
Identity = 38/89 (42.70%), Postives = 50/89 (56.18%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVD  S  SV SK++FG+D  LL HP LN+T+F      + DTFYYV +K V+V GE L+I   TW + ++              YFA
Sbjct:  312 CLVDHGS--SVGSKIVFGDDDALLGHPRLNYTAFAPSAAAAADTFYYVQLKGVLVGGEKLNISPSTWDVGKDGSGGTIIDSGTTLSYFA 398
[Close]
BLAST of EX570110 vs. TAIR peptide
Match: AT3G59080.2 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:21836812-21838419 FORWARD LENGTH=499)

HSP 1 Score: 122.479 bits (306), Expect = 5.373e-29
Identity = 59/89 (66.29%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LLSHPNLNFTSFV G+EN VDTFYYV IKS++V GE L+IPEETW +S +              YFA
Sbjct:  296 CLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 384
[Close]
BLAST of EX570110 vs. TAIR peptide
Match: AT3G59080.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr3:21836812-21838419 FORWARD LENGTH=535)

HSP 1 Score: 122.479 bits (306), Expect = 5.373e-29
Identity = 59/89 (66.29%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNSD++VSSKLIFGEDK+LLSHPNLNFTSFV G+EN VDTFYYV IKS++V GE L+IPEETW +S +              YFA
Sbjct:  332 CLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 420
[Close]
BLAST of EX570110 vs. TAIR peptide
Match: AT2G42980.1 (| Symbols: | Eukaryotic aspartyl protease family protein | chr2:17875005-17876588 REVERSE LENGTH=527)

HSP 1 Score: 116.701 bits (291), Expect = 2.948e-27
Identity = 55/89 (61.80%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:    2 CLVDRNSDSSVSSKLIFGEDKELLSHPNLNFTSFVGGEENSVDTFYYVGIKSVMVDGEALSIPEETWRLSEEXXXXXXXXXXXXXXYFA 268
            CLVDRNS+++VSSKLIFGEDK+LL+H NLNFTSFV G+ENSV+TFYY+ IKS++V G+AL IPEETW +S +              YFA
Sbjct:  322 CLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFA 410
The following BLAST results are available for this feature:
BLAST of EX570110 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9LYS8_ARATH1.376e-2666.29AT3g59080/F17J16_130 OS=Arabidopsis thaliana GN=F1... [more]
Q2V3N3_ARATH1.376e-2666.29Putative uncharacterized protein At3g59080.2 OS=Ar... [more]
D7LJF0_ARALY8.921e-2664.04Putative uncharacterized protein OS=Arabidopsis ly... [more]
D7LWA4_ARALY1.522e-2565.17Putative uncharacterized protein (Fragment) OS=Ara... [more]
B9S302_RICCO1.987e-2565.17Basic 7S globulin 2 small subunit, putative OS=Ric... [more]
Q9SJG1_ARATH7.552e-2561.80Putative chloroplast nucleoid DNA binding protein ... [more]
B9H837_POPTR1.682e-2472.22Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q5MJW8_TOBAC1.802e-1863.16Avr9/Cf-9 rapidly elicited protein 36 (Fragment) O... [more]
Q6Z6Q8_ORYSJ1.929e-1242.70Os02g0314600 protein OS=Oryza sativa subsp. japoni... [more]
A2X403_ORYSI1.929e-1242.70Putative uncharacterized protein OS=Oryza sativa s... [more]
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BLAST of EX570110 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT3G59080.25.373e-2966.29| Symbols: | Eukaryotic aspartyl protease family ... [more]
AT3G59080.15.373e-2966.29| Symbols: | Eukaryotic aspartyl protease family ... [more]
AT2G42980.12.948e-2761.80| Symbols: | Eukaryotic aspartyl protease family ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Peptidase A1PANTHERPTHR13683ASPARTYL PROTEASEScoord: 1..89
score: 2.9
IPR009007Peptidase aspartic, catalyticGENE3D2.40.70.10no descriptioncoord: 25..88
score: 1.6
IPR021109Peptidase asparticSUPERFAMILY50630Acid proteasescoord: 1..89
score: 3.3
NoneNo IPR availablePANTHERPTHR13683:SF98CHLOROPLAST NUCLEIOD DNA-BINDING-RELATEDcoord: 1..89
score: 2.9
NoneNo IPR availableSEGsegsegcoord: 73..86
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX570110 ID=EX570110; Name=EX570110; organism=Pisum sativum; type=EST; length=268bp
TTGTCTCGTCGATAGAAACAGCGACTCGAGTGTTAGTAGTAAGTTGATAT
TCGGCGAGGATAAGGAACTTCTTAGCCATCCGAATTTGAACTTCACTTCT
TTCGTTGGTGGCGAAGAGAATTCGGTCGATACGTTTTACTACGTCGGGAT
AAAATCGGTTATGGTTGATGGCGAAGCGTTGAGTATACCAGAAGAAACTT
GGCGTTTATCAGAAGAAGGTTCTGGTGGTACTATAATTGATTCTGGAACA
ACTTTAACTTATTTTGCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR009007Peptidase aspartic, catalytic
IPR021109Peptidase_aspartic_dom_sf