EX571096

EST Overview


Name	EX571096
Unique Name	EX571096
Type	EST
Organism	Pisum sativum (pea)
Sequence length	620

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX571096 vs. TrEMBL
Match: C6TJ53_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.445e-74
Identity = 132/158 (83.54%), Postives = 149/158 (94.30%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHG+Y+P+DLSLLPPLYLIY  NPSDSGKVHSKVRQRWLDGDEFIVS+MLE+ANIA+EG+TALEEKDY K A+LMN+NFDLRRLMFGDDALGD+N+KMVE+ RKVGAASKFTGSGGAVVA+CPEGTSQVKLLEDEC + GFV++P+EP P RLNEID
Sbjct:  193 LGHGVYVPVDLSLLPPLYLIYAENPSDSGKVHSKVRQRWLDGDEFIVSSMLEIANIAQEGKTALEEKDYSKFAALMNRNFDLRRLMFGDDALGDLNLKMVEVARKVGAASKFTGSGGAVVAFCPEGTSQVKLLEDECQKEGFVILPIEPLPSRLNEID 350

[Close]

BLAST of EX571096 vs. TrEMBL
Match: D7TWH1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_66.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032385001 PE=4 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 4.820e-62
Identity = 115/158 (72.78%), Postives = 135/158 (85.44%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIYIPMD+SLLPPLYLIY  NPSDSGKVHS V+QRWL+GDEFI+ +M EVAN+A EGRTAL EKDY K A LMN NFDLRR MFG+D LG +N++MVE+ R+VGAASKFTGSGGAVVA+CP+G SQVKLLED C +AGF++ P+E  P  L++ID
Sbjct:  196 LGHGIYIPMDISLLPPLYLIYAENPSDSGKVHSTVQQRWLNGDEFIIKSMAEVANLASEGRTALLEKDYSKFAYLMNHNFDLRRRMFGEDVLGALNVEMVEVARRVGAASKFTGSGGAVVAFCPDGPSQVKLLEDACQKAGFIIQPVEVKPSCLSDID 353

[Close]

BLAST of EX571096 vs. TrEMBL
Match: B9GIJ6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_549568 PE=3 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.551e-60
Identity = 114/158 (72.15%), Postives = 133/158 (84.18%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHG+Y PMD SLLPPL LIY  NPSDSGKVHS V++RWLDGDEFIVS+M EVA++A +G+TA+ EKDY KLA LMN+NFDLRR MFGDDALG +NI+MVE+ R+VGAASKFTGSGGAVV +CP+G  QVKLLED C EAGFV+ P++  P  LNE D
Sbjct:  198 LGHGVYTPMDTSLLPPLQLIYAENPSDSGKVHSTVQKRWLDGDEFIVSSMAEVADLALQGQTAILEKDYSKLADLMNRNFDLRRSMFGDDALGSLNIEMVEVARRVGAASKFTGSGGAVVVFCPDGPPQVKLLEDACKEAGFVIQPVKVVPSYLNEDD 355

[Close]

BLAST of EX571096 vs. TrEMBL
Match: D7L9M7_ARALY (GHMP kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_477351 PE=4 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 2.025e-60
Identity = 113/158 (71.52%), Postives = 133/158 (84.18%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIY PMD+SLLPPL+LIY  NPSDSGKVHS VRQRWLDGDEFI+S+M EV N+AEEGRTAL  KD+ KL  LMN NFD+RR MFGD+ LG +NI+MVE+ R+VGAASKFTGSGGAVV +CPEG SQVKLLE+EC ++GF+L P++  P  LN+ D
Sbjct:  201 LGHGIYTPMDISLLPPLHLIYAENPSDSGKVHSMVRQRWLDGDEFIISSMKEVGNLAEEGRTALLNKDHSKLVELMNLNFDIRRRMFGDECLGAMNIEMVEVARRVGAASKFTGSGGAVVVFCPEGPSQVKLLEEECRKSGFMLQPVKIAPSCLNDSD 358

[Close]

BLAST of EX571096 vs. TrEMBL
Match: Q9SS91_ARATH (F4P13.18 protein OS=Arabidopsis thaliana GN=F4P13.18 PE=3 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.512e-60
Identity = 113/158 (71.52%), Postives = 132/158 (83.54%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIY PMD+SLLPPL+LIY  NPSDSGKVHS VRQRWLDGDEFI+S+M EV ++AEEGRTAL  KD+ KL  LMN NFD+RR MFGD+ LG +NI+MVE+ R+VGAASKFTGSGGAVV +CPEG SQVKLLE+EC +AGF L P++  P  LN+ D
Sbjct:  107 LGHGIYTPMDISLLPPLHLIYAENPSDSGKVHSMVRQRWLDGDEFIISSMKEVGSLAEEGRTALLNKDHSKLVELMNLNFDIRRRMFGDECLGAMNIEMVEVARRVGAASKFTGSGGAVVVFCPEGPSQVKLLEEECRKAGFTLQPVKIAPSCLNDSD 264

[Close]

BLAST of EX571096 vs. TrEMBL
Match: Q93ZC9_ARATH (AT3g01640/F4P13_18 OS=Arabidopsis thaliana GN=GlcAK1 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.512e-60
Identity = 113/158 (71.52%), Postives = 132/158 (83.54%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIY PMD+SLLPPL+LIY  NPSDSGKVHS VRQRWLDGDEFI+S+M EV ++AEEGRTAL  KD+ KL  LMN NFD+RR MFGD+ LG +NI+MVE+ R+VGAASKFTGSGGAVV +CPEG SQVKLLE+EC +AGF L P++  P  LN+ D
Sbjct:  201 LGHGIYTPMDISLLPPLHLIYAENPSDSGKVHSMVRQRWLDGDEFIISSMKEVGSLAEEGRTALLNKDHSKLVELMNLNFDIRRRMFGDECLGAMNIEMVEVARRVGAASKFTGSGGAVVVFCPEGPSQVKLLEEECRKAGFTLQPVKIAPSCLNDSD 358

[Close]

BLAST of EX571096 vs. TrEMBL
Match: D7M6J8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_325917 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.111e-58
Identity = 107/158 (67.72%), Postives = 134/158 (84.81%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            +G+GIY  MD++LLPPL+LIY  NPSDSGKVHS VR+RWLDGDEFI+S+M E+A +AEEGRTAL +KDY KL  LMN+NFDLRR MFGD+ LG +NI+MVE+ RK+GAA+KFTGSGGAVV +CP+G SQVKLLE+EC ++GF++ P++  P RLN  D
Sbjct:  202 VGYGIYTIMDINLLPPLHLIYAENPSDSGKVHSTVRRRWLDGDEFIISSMAEIAKLAEEGRTALLKKDYSKLKELMNRNFDLRRSMFGDECLGAMNIEMVEVARKIGAAAKFTGSGGAVVVFCPDGPSQVKLLEEECRKSGFIVEPVKLVPTRLNNSD 359

[Close]

BLAST of EX571096 vs. TrEMBL
Match: B9S667_RICCO (ATP binding protein, putative OS=Ricinus communis GN=RCOM_1065080 PE=3 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 5.515e-58
Identity = 110/158 (69.62%), Postives = 129/158 (81.65%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIY PMD+ LLPPL+LIY  NPSDSGKVHS VRQRWL+GD+FI+S M EVA++A EGRTA+ EK+Y KLA LMN NFDLRR MFGDD +G +NI+MVE+ R+VGAASKFTGSGGAVV YCP G SQ+KLLED C  AGF + P++  P  L+E D
Sbjct:  192 LGHGIYTPMDIELLPPLHLIYAENPSDSGKVHSTVRQRWLNGDKFIISLMAEVADVALEGRTAILEKNYAKLADLMNLNFDLRRSMFGDDVIGALNIEMVEVARRVGAASKFTGSGGAVVVYCPGGPSQIKLLEDACQLAGFTIQPVQVVPSCLHEDD 349

[Close]

BLAST of EX571096 vs. TrEMBL
Match: Q9LY82_ARATH (Putative uncharacterized protein F18O22_260 OS=Arabidopsis thaliana GN=F18O22_260 PE=3 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 7.203e-58
Identity = 106/158 (67.09%), Postives = 133/158 (84.18%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            +G+GIY  MD++LLPPL+LIY  NPSDSGKVHS VR+RWLDGDEFI+S+M E+A +AEEGRTAL +KDY  L  LMN+NFDLRR MFGD+ LG +NI+MVE+ RK+GAA+KFTGSGGAVV +CPEG SQVKLLE+EC ++GF++ P++  P RL+  D
Sbjct:  202 VGYGIYTIMDINLLPPLHLIYAENPSDSGKVHSTVRRRWLDGDEFIISSMAEIAKLAEEGRTALLKKDYSNLKELMNRNFDLRRSMFGDECLGAMNIEMVEVARKIGAAAKFTGSGGAVVVFCPEGPSQVKLLEEECRKSGFIVEPVKLVPTRLSSSD 359

[Close]

BLAST of EX571096 vs. TrEMBL
Match: Q5PP60_ARATH (At5g14470 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 7.203e-58
Identity = 106/158 (67.09%), Postives = 133/158 (84.18%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            +G+GIY  MD++LLPPL+LIY  NPSDSGKVHS VR+RWLDGDEFI+S+M E+A +AEEGRTAL +KDY  L  LMN+NFDLRR MFGD+ LG +NI+MVE+ RK+GAA+KFTGSGGAVV +CPEG SQVKLLE+EC ++GF++ P++  P RL+  D
Sbjct:  108 VGYGIYTIMDINLLPPLHLIYAENPSDSGKVHSTVRRRWLDGDEFIISSMAEIAKLAEEGRTALLKKDYSNLKELMNRNFDLRRSMFGDECLGAMNIEMVEVARKIGAAAKFTGSGGAVVVFCPEGPSQVKLLEEECRKSGFIVEPVKLVPTRLSSSD 265

[Close]

BLAST of EX571096 vs. TAIR peptide
Match: AT3G01640.1 (| Symbols: ATGLCAK, GLCAK | glucuronokinase G | chr3:239418-241198 FORWARD LENGTH=362)

HSP 1 Score: 234.572 bits (597), Expect = 3.301e-62
Identity = 113/158 (71.52%), Postives = 132/158 (83.54%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            LGHGIY PMD+SLLPPL+LIY  NPSDSGKVHS VRQRWLDGDEFI+S+M EV ++AEEGRTAL  KD+ KL  LMN NFD+RR MFGD+ LG +NI+MVE+ R+VGAASKFTGSGGAVV +CPEG SQVKLLE+EC +AGF L P++  P  LN+ D
Sbjct:  201 LGHGIYTPMDISLLPPLHLIYAENPSDSGKVHSMVRQRWLDGDEFIISSMKEVGSLAEEGRTALLNKDHSKLVELMNLNFDIRRRMFGDECLGAMNIEMVEVARRVGAASKFTGSGGAVVVFCPEGPSQVKLLEEECRKAGFTLQPVKIAPSCLNDSD 358

[Close]

BLAST of EX571096 vs. TAIR peptide
Match: AT5G14470.1 (| Symbols: | GHMP kinase family protein | chr5:4663029-4664657 REVERSE LENGTH=366)

HSP 1 Score: 227.254 bits (578), Expect = 5.270e-60
Identity = 106/158 (67.09%), Postives = 133/158 (84.18%), Query Frame = 2

Query:    2 LGHGIYIPMDLSLLPPLYLIYVANPSDSGKVHSKVRQRWLDGDEFIVSTMLEVANIAEEGRTALEEKDYPKLASLMNKNFDLRRLMFGDDALGDVNIKMVEIVRKVGAASKFTGSGGAVVAYCPEGTSQVKLLEDECHEAGFVLIPLEPFPFRLNEID 475
            +G+GIY  MD++LLPPL+LIY  NPSDSGKVHS VR+RWLDGDEFI+S+M E+A +AEEGRTAL +KDY  L  LMN+NFDLRR MFGD+ LG +NI+MVE+ RK+GAA+KFTGSGGAVV +CPEG SQVKLLE+EC ++GF++ P++  P RL+  D
Sbjct:  202 VGYGIYTIMDINLLPPLHLIYAENPSDSGKVHSTVRRRWLDGDEFIISSMAEIAKLAEEGRTALLKKDYSNLKELMNRNFDLRRSMFGDECLGAMNIEMVEVARKIGAAAKFTGSGGAVVVFCPEGPSQVKLLEEECRKSGFIVEPVKLVPTRLSSSD 359

The following BLAST results are available for this feature:

BLAST of EX571096 vs. TrEMBL
BLAST of EX571096 vs. TAIR peptide

BLAST of EX571096 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TJ53_SOYBN	1.445e-74	83.54	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TWH1_VITVI	4.820e-62	72.78	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GIJ6_POPTR	1.551e-60	72.15	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7L9M7_ARALY	2.025e-60	71.52	GHMP kinase family protein OS=Arabidopsis lyrata s...	[more]
Q9SS91_ARATH	4.512e-60	71.52	F4P13.18 protein OS=Arabidopsis thaliana GN=F4P13....	[more]
Q93ZC9_ARATH	4.512e-60	71.52	AT3g01640/F4P13_18 OS=Arabidopsis thaliana GN=GlcA...	[more]
D7M6J8_ARALY	1.111e-58	67.72	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9S667_RICCO	5.515e-58	69.62	ATP binding protein, putative OS=Ricinus communis ...	[more]
Q9LY82_ARATH	7.203e-58	67.09	Putative uncharacterized protein F18O22_260 OS=Ara...	[more]
Q5PP60_ARATH	7.203e-58	67.09	At5g14470 OS=Arabidopsis thaliana PE=2 SV=1	[more]

BLAST of EX571096 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G01640.1	3.301e-62	71.52	\| Symbols: ATGLCAK, GLCAK \| glucuronokinase G \| ch...	[more]
AT5G14470.1	5.270e-60	67.09	\| Symbols: \| GHMP kinase family protein \| chr5:46...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013750	GHMP kinase, C-terminal	PFAM	PF08544	GHMP_kinases_C	coord: 62..127 score: 5.3
None	No IPR available	GENE3D	3.30.70.890	no description	coord: 17..150 score: 8
None	No IPR available	SUPERFAMILY	55060	GHMP Kinase, C-terminal domain	coord: 4..150 score: 3.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX571096 ID=EX571096; Name=EX571096; organism=Pisum sativum; type=EST; length=620bp
ATTAGGGCACGGAATTTATATACCAATGGATTTGAGTCTACTCCCACCTC
TATACCTCATCTATGTAGCGAATCCTAGCGACTCTGGGAAGGTTCATAGT
AAAGTACGTCAGAGATGGCTTGATGGTGATGAGTTCATCGTGTCAACTAT
GCTTGAAGTGGCAAATATTGCGGAAGAAGGAAGGACGGCACTAGAAGAGA
AGGACTATCCCAAACTAGCATCTCTCATGAATAAAAATTTTGACCTGCGA
AGGTTGATGTTCGGAGATGATGCACTTGGTGATGTGAACATAAAAATGGT
AGAGATTGTTAGAAAGGTTGGAGCGGCGTCGAAATTTACAGGTAGCGGGG
GAGCTGTTGTTGCATATTGTCCTGAAGGAACTTCTCAAGTAAAGCTTCTT
GAGGATGAATGTCATGAAGCAGGATTTGTTTTAATACCTCTTGAACCTTT
TCCTTTTCGGTTAAATGAAATAGACTTGAAAACCCTGCAGTTGTAATGTA
CTATTCTTACTCTGACGATTTGAAAATTAGTTGTTATGAGTTAGAATTTT
CAATATGAGATGTTCTTTTTATTTATGAATTCAAAATAAAAACAAATGGG
TTGAATATGAATTGATTTTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR013750	GHMP_kinase_C_dom

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