FG534552

EST Overview


Name	FG534552
Unique Name	FG534552
Type	EST
Organism	Pisum sativum (pea)
Sequence length	620

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG534552 vs. TrEMBL
Match: B9SFJ3_RICCO (WD-repeat protein, putative OS=Ricinus communis GN=RCOM_0646590 PE=4 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 3.802e-75
Identity = 143/205 (69.76%), Postives = 168/205 (81.95%), Query Frame = -1

Query:    3 AAETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 608
            AAE  K+ EVAPALI+VHP ++S AVAVG +LRVF+L G C V LVD++    +P HKD+IRAIR+ A GKLFVSAGDDK + IWST    CVS+V SEKRV+AVAISN+GL+VCFADKFG+VWVVDL++   ++ L     +PLL+HYCSIITSLEFSPD RFI+SADRDFKIRVT FPK  L+GAHEIQSFCLGHTEFVSCLA
Sbjct:   10 AAENNKEIEVAPALISVHPTQNSVAVAVGSDLRVFDLQGGCGVCLVDNN--SSEPLHKDSIRAIRYSANGKLFVSAGDDKLVNIWSTDSWGCVSSVCSEKRVSAVAISNDGLYVCFADKFGVVWVVDLHELEGNQSLVNKKASPLLAHYCSIITSLEFSPDGRFIVSADRDFKIRVTVFPKKTLDGAHEIQSFCLGHTEFVSCLA 212

[Close]

BLAST of FG534552 vs. TrEMBL
Match: B9H8A4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1079098 PE=4 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 5.490e-74
Identity = 145/205 (70.73%), Postives = 169/205 (82.44%), Query Frame = -1

Query:    3 ETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDL-----NQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 602
            E  +D EVAPALI+VHP + S AVAVG +LR F+L GNC V+LVD+  TGE  FHKD+IRAIR+GAKGKLFVSAGDDK +K+WST    C+++V SEKRV+AVAISN+GL+VCFADKFG+VWVVDL     N+T  +KK    PLL+HYCSIITSLEFSPD  FI++ADRDFKIRVT FPK  L+GAHEIQSFCLGHTEFVSCLA
Sbjct:   10 ENNRDVEVAPALISVHPTQDSVAVAVGSDLRFFDLRGNCGVTLVDE--TGEA-FHKDSIRAIRYGAKGKLFVSAGDDKLVKVWSTDSWRCIASVCSEKRVSAVAISNDGLYVCFADKFGVVWVVDLRGLDGNETLVNKK--AAPLLAHYCSIITSLEFSPDGWFIVTADRDFKIRVTVFPKKPLDGAHEIQSFCLGHTEFVSCLA 209

[Close]

BLAST of FG534552 vs. TrEMBL
Match: D7TDY8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_151.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001175001 PE=4 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 2.819e-70
Identity = 138/205 (67.32%), Postives = 161/205 (78.54%), Query Frame = -1

Query:    3 ETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDL-----NQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 602
            +  +D EVAPALI+VHPD     V++G +LRVF+L G C+V+LVDDS     P HKD+IRAIRFGA+GKLFVSAGDDK +KIW+     C+STV SEKRV+AVAIS +GL VCFADKFG+VW ++L     NQ   DKK    P+L+HYCSIITSLEFS D RFIISADRDFKIRV+ FPK  L+GAHEIQSFCLGHTEFVSCLA
Sbjct:   10 DNHRDVEVAPALISVHPDHKFIVVSIGSDLRVFDLQGGCSVTLVDDS---SGPLHKDSIRAIRFGAEGKLFVSAGDDKLVKIWNANSWHCISTVCSEKRVSAVAISKDGLFVCFADKFGVVWALNLDGFDENQVLVDKK--AAPVLAHYCSIITSLEFSLDGRFIISADRDFKIRVSVFPKKPLDGAHEIQSFCLGHTEFVSCLA 209

[Close]

BLAST of FG534552 vs. TrEMBL
Match: D7KCB5_ARALY (Transducin family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_887463 PE=4 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.891e-66
Identity = 134/208 (64.42%), Postives = 162/208 (77.88%), Query Frame = -1

Query:    3 EEVAAETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTP---LLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 617
            EEV  E +   EVAPALI+VHP + S AVAVG +LRVFNL+ N  VSLVD+S   + P  K++IRAIR+ A GKLFVSAGDDK +KIWST    C++TV SEKRV+AVAIS++  HVC+ADKFG+VWV++L+  ++ K L       LLSHYCSIITSLEFSPD R+I+SADRDFKIRVT FPK  L GAHEIQSFCLGH+EF++C A
Sbjct:    8 EEV--EIQNKLEVAPALISVHPSQKSLAVAVGSDLRVFNLIENRPVSLVDES---DGPSRKESIRAIRYSASGKLFVSAGDDKLVKIWSTDSWRCLNTVCSEKRVSAVAISSDDSHVCYADKFGVVWVIELDGINEGKTLPSKKGALLLSHYCSIITSLEFSPDGRYILSADRDFKIRVTVFPKKPLEGAHEIQSFCLGHSEFITCTA 210

[Close]

BLAST of FG534552 vs. TrEMBL
Match: Q93WD7_ARATH (Putative uncharacterized protein At1g03110 OS=Arabidopsis thaliana GN=At1g03110 PE=2 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.226e-66
Identity = 131/208 (62.98%), Postives = 160/208 (76.92%), Query Frame = -1

Query:    3 EEVAAETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTP---LLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 617
            EEV  E +   EVAPALI+VHP + S AVAVG +LR+F+L+ NC VSLVD+S   + P  K++IRAIR+   GKLFVSAGDDK +KIWS     C++TV SEKRV+AVAIS++  HVC+ADKFG+VWV++L+  +  K L       LLSHYCSIITSLEFSPD R+I+SADRDFKIRVT FPK  L GAHEIQSFCLGH+EF++C A
Sbjct:    8 EEV--EIQNKLEVAPALISVHPSQKSVAVAVGSDLRIFDLIENCPVSLVDES---DGPIRKESIRAIRYSTSGKLFVSAGDDKLVKIWSADSWRCLNTVCSEKRVSAVAISSDDSHVCYADKFGVVWVIELDGINDGKTLPSKKGALLLSHYCSIITSLEFSPDGRYILSADRDFKIRVTVFPKKPLEGAHEIQSFCLGHSEFITCTA 210

[Close]

BLAST of FG534552 vs. TrEMBL
Match: Q94GP6_ORYSJ (Os03g0746800 protein OS=Oryza sativa subsp. japonica GN=OJ1124_H03.17 PE=4 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 6.960e-61
Identity = 118/197 (59.90%), Postives = 147/197 (74.62%), Query Frame = -1

Query:    3 EVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 584
            E APAL+A HP   S AVAVGPELRVF+L  + AVSL D+S  G    H D IRAI F A G LF SAGDDK +K+W T    C+ T++SEKRV+AVAISN+G +V FADKFG++W+V + ++  +++ T   P  +  HYCSIITS++FSPD RFI +ADRDFKIR+T+ PK  L GAHEIQSFCLGHT+FVSC+A
Sbjct:   17 EFAPALVAAHPLGRSVAVAVGPELRVFDLKASSAVSLSDNSGGGS---HSDAIRAISFSANGALFASAGDDKLVKVWKTDSWCCIRTITSEKRVSAVAISNDGTYVTFADKFGVIWLVTMGESGGEQEPTDNKPVSIFGHYCSIITSMKFSPDGRFIATADRDFKIRITSLPKKPLRGAHEIQSFCLGHTDFVSCIA 210

[Close]

BLAST of FG534552 vs. TrEMBL
Match: B8AJV3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_13488 PE=4 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 6.960e-61
Identity = 118/197 (59.90%), Postives = 147/197 (74.62%), Query Frame = -1

Query:    3 EVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 584
            E APAL+A HP   S AVAVGPELRVF+L  + AVSL D+S  G    H D IRAI F A G LF SAGDDK +K+W T    C+ T++SEKRV+AVAISN+G +V FADKFG++W+V + ++  +++ T   P  +  HYCSIITS++FSPD RFI +ADRDFKIR+T+ PK  L GAHEIQSFCLGHT+FVSC+A
Sbjct:   17 EFAPALVAAHPLGRSVAVAVGPELRVFDLKASSAVSLSDNSGGGS---HSDAIRAISFSANGALFASAGDDKLVKVWKTDSWCCIRTITSEKRVSAVAISNDGTYVTFADKFGVIWLVTMGESGGEQEPTDNKPVSIFGHYCSIITSMKFSPDGRFIATADRDFKIRITSLPKKPLRGAHEIQSFCLGHTDFVSCIA 210

[Close]

BLAST of FG534552 vs. TrEMBL
Match: B6TK73_MAIZE (WD-repeat protein 4 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.512e-60
Identity = 119/196 (60.71%), Postives = 142/196 (72.45%), Query Frame = -1

Query:    6 EVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 584
            E APAL+A HP  +S AVA+GPELRVF+L     VSL DD        H D IRAI F   G LF SAGDDK +K+W T    C+ T++SEKRV+AV ISN+ L+V FADKFG+VW+V L + S ++      P P+L HYCSIITS++FSPD RFI +ADRDFKIRVT FPKN L GAHEIQSFCLGHT+FVSC+
Sbjct:   16 EFAPALVAAHPHGNSVAVAIGPELRVFDLRAGHPVSLSDDCGGHS---HSDAIRAICFSVSGALFASAGDDKLVKVWKTDSWRCIQTITSEKRVSAVVISNDDLYVSFADKFGVVWLVTLREDSAEQVSVDNKPVPILGHYCSIITSMKFSPDGRFIATADRDFKIRVTLFPKNPLKGAHEIQSFCLGHTDFVSCI 208

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BLAST of FG534552 vs. TrEMBL
Match: C5X165_SORBI (Putative uncharacterized protein Sb01g008280 OS=Sorghum bicolor GN=Sb01g008280 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.714e-59
Identity = 118/196 (60.20%), Postives = 141/196 (71.94%), Query Frame = -1

Query:    6 EVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 584
            E APAL+A HP  +S AVA+GPELR F+L     VSL DD        H D IRAI F   G LF SAGDDK +K+W T    C+ T++SEKRV+AVAISN+ L+V FADKFG+VW+V L + S ++      P  +L HYCSIITS++FSPD RFI +ADRDFKIRVT FPKN L GAHEIQSFCLGHT+FVSC+
Sbjct:   16 EFAPALVAAHPQGNSVAVAIGPELRAFDLSAGLPVSLSDDCGGRS---HSDAIRAICFSVSGALFASAGDDKLVKVWKTDSWRCIQTITSEKRVSAVAISNDDLYVTFADKFGVVWLVTLREDSAEQVSVDNKPVSILGHYCSIITSMKFSPDGRFIATADRDFKIRVTLFPKNPLKGAHEIQSFCLGHTDFVSCI 208

[Close]

BLAST of FG534552 vs. TrEMBL
Match: C0P5K9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.819e-59
Identity = 118/196 (60.20%), Postives = 141/196 (71.94%), Query Frame = -1

Query:    6 EVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLT---PTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 584
            E APAL+A HP  +S AVA+GPELRVF+L     VSL DD        H D IRAI F   G LF SAGDDK +K+W T    C+ T++SEKRV+AV ISN+ L+V FADKFG+VW+V L + S ++      P  +L HYCSIITS++FSPD RFI +ADRDFKIRVT FPKN L GAHEIQSFCLGHT+FVSC+
Sbjct:   16 EFAPALVAAHPHGNSVAVAIGPELRVFDLRAGHPVSLSDDCGGHS---HSDAIRAICFSVSGALFASAGDDKLVKVWKTDSWRCIQTITSEKRVSAVVISNDDLYVSFADKFGVVWLVTLREDSAEQVSVDNKPVSILGHYCSIITSMKFSPDGRFIATADRDFKIRVTLFPKNPLKGAHEIQSFCLGHTDFVSCI 208

[Close]

BLAST of FG534552 vs. SwissProt
Match: TRM82_YARLI (tRNA (guanine-N(7)-)-methyltransferase subunit TRM82 OS=Yarrowia lipolytica GN=TRM82 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 7.682e-15
Identity = 63/212 (29.72%), Postives = 107/212 (50.47%), Query Frame = -1

Query:    6 LIAVHPDRHSAAVAVGPEL-RVFNLLGNCAVSLVDDSVT------GEQPFHK----------DNIRAIRFGAKGKLFVSAGDD-KTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVW---VVDLNQTSQDKKLTPTPLLSHYCSIITSLEF---SPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 569
            ++++ PD  +   A   ++ R+    G C  ++V++S T      G++P              ++R+++    GK  ++  D+ K+L +      + +  V   KR +AVA   E L++   DKFG V+   V DL +   + +L   P+L H  S++T+LE    S D  ++ISADRD  IR++ FP      +H I+ FC GH +FVS L
Sbjct:    7 IVSLSPDGKTMYAAAARQVDRICLETGECKSTVVEESATRTTTKTGDKPAAAKRTKYLPTSVSHVRSLQLSKGGKYLLATTDETKSLVLLDPTTLEVIKKVQYPKRPSAVATDKEDLNIVLGDKFGDVYGESVEDLLKV--EGELKDEPVLGH-VSMLTALELGYDSKDRPYVISADRDEHIRISRFP-----ASHVIEQFCFGHQQFVSSL 210

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_XENTR (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Xenopus tropicalis GN=wdr4 PE=2 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.109e-13
Identity = 53/148 (35.81%), Postives = 76/148 (51.35%), Query Frame = -1

Query:    6 PFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQ-CQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 446
            P   D I A  F   G+ F    D+K L ++ T+   + +S     +R TA+  S  G H+  ADK G V+   + ++ +  +L     L H  S++  +  SPD   II+ DRD KIRV+ +     +  H I SFCLGHTEFVS L
Sbjct:   51 PQGSDKILAAAFSPSGEYFALTDDNKRLILFQTKPAWETISVRWVSRRCTALTFSPCGAHILVADKSGDVFSFSVRRSREAGRLE----LGHL-SMLLDVAVSPDGNHIITCDRDEKIRVSRW-----DSPHVIMSFCLGHTEFVSQL 188

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_DICDI (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Dictyostelium discoideum GN=wdr4 PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.109e-13
Identity = 56/209 (26.79%), Postives = 103/209 (49.28%), Query Frame = -1

Query:   12 DTEVAPALIAVHP-DRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQ-DCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQ--------------DKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVS 590
            + E  P  I  H  D +  A  +  +L +F+      ++ + +  +G Q  H   IR+I F    +  +++  DK LKIW T      + ++++ K++    ++ +   +  +DK G V+   L   S+              +K+     +  HY SI+  ++FSP   +++SADRD KIRV+++P        +I+SFCLGHT++V+
Sbjct:   17 NVEKVPISIITHSSDSNFIAYRLNNKLNIFDN----KLNKLGELESGSQ--HTGIIRSIEFTKNNQSLITSSSDKFLKIWDTTNNFKNIKSINTNKKIICSILNKDDSEILVSDKCGDVFKYSLIDDSKNKIEVSGDKSAKHDEKESDKNLVFGHYSSIV-DIKFSPCFNYLLSADRDEKIRVSHYP-----NCFDIESFCLGHTKYVT 213

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_BOVIN (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Bos taurus GN=WDR4 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.449e-13
Identity = 50/141 (35.46%), Postives = 72/141 (51.06%), Query Frame = -1

Query:   12 DNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVS 434
            D + A  F   G  FV   D K L ++ T    C+S  +  +R TA+  +     +  ADK G V+   + +     +L     L H  S++  +  SPD+RF+++ADRD KIRV     +     H I+SFCLGHTEFVS
Sbjct:   63 DTVLASTFSKSGSYFVLTDDSKRLILFRTNPWQCLSVRTVVRRCTALTFTASEEKILVADKSGDVYSFSVLEPHGGGRLE----LGHL-SMLLDVAVSPDDRFVLTADRDEKIRV-----SWAAAPHSIESFCLGHTEFVS 193

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_MOUSE (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Mus musculus GN=Wdr4 PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 5.506e-13
Identity = 50/141 (35.46%), Postives = 73/141 (51.77%), Query Frame = -1

Query:   12 DNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVS 434
            D+I A  F   G+ F    D K L ++ T+   C+S     +R TA+  +     V  ADK G V+   + +     +L     L H  S++  +  SPD++F+++ADRD KIRV     +     H I+SFCLGHTEFVS
Sbjct:   64 DSILASTFSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCGRLE----LGH-LSMLLDVAVSPDDQFVLTADRDEKIRV-----SWAAAPHSIESFCLGHTEFVS 194

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_DANRE (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Danio rerio GN=wdr4 PE=2 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 7.190e-13
Identity = 56/159 (35.22%), Postives = 82/159 (51.57%), Query Frame = -1

Query:    6 VDDSVTGEQPFHKDN--IRAIRFGAKGKLFVSAGDDKTLKIWSTQCQ-DCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 473
            VD+   GE    KD+  I A    A GK      D K L ++ T+    C+ST    +R T++A +     +  ADK G V+   + +  +  +L     L H  S++  +  SPD+++II+ADRD KIRV     +     + IQ+FCLGHTEFVS L
Sbjct:   47 VDNKSAGEGSEEKDSDSILAFAISASGKHVALTDDHKRLVLFCTEPSWKCISTRWVVRRCTSLAFTQAEDELYVADKSGDVYSFSILEPHKAGELK----LGHL-SMLLDVALSPDDKYIITADRDEKIRV-----SFRRSPYNIQAFCLGHTEFVSSL 195

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_HUMAN (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Homo sapiens GN=WDR4 PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 2.092e-12
Identity = 51/139 (36.69%), Postives = 70/139 (50.36%), Query Frame = -1

Query:   12 IRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVS 428
            I A  F   G  F    D K L ++ T+   C+S  +  +R TA+        V  ADK G V+   + +     +L     L H  S++  +  SPD+RFI++ADRD KIRV     +     H I+SFCLGHTEFVS
Sbjct:   64 ILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTVARRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLE----LGHL-SMLLDVAVSPDDRFILTADRDEKIRV-----SWAAAPHSIESFCLGHTEFVS 192

[Close]

BLAST of FG534552 vs. SwissProt
Match: WDR4_XENLA (tRNA (guanine-N(7)-)-methyltransferase subunit WDR4 OS=Xenopus laevis GN=wdr4 PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 3.569e-12
Identity = 61/197 (30.96%), Postives = 92/197 (46.70%), Query Frame = -1

Query:    6 VAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQ----PFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQ-CQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 581
            V P  +A+          VG E   F+L  +C++     +  G++        D I A  F   G+ F    D+K L ++ T+   + +S     +R TA+  S  G H+  ADK G V+   + +  +  +L     L H  S++  +  S D + II+ DRD KIRV+ +        H I SFCLGHTEFVS L
Sbjct:    4 VGPGSLAITGGSRLLGHRVGSECCPFHL--DCSLLEKQSAAPGQEGSADSHGSDKILAAAFSPSGEYFALTDDNKRLVLFRTKPAWEKISVRWVSRRCTALTFSPCGNHILVADKSGDVFSFSVPRALEQGRLE----LGHL-SMLLDVTVSLDGKHIITCDRDEKIRVSCW-----GAPHVIMSFCLGHTEFVSQL 188

[Close]

BLAST of FG534552 vs. SwissProt
Match: TRM82_PICGU (tRNA (guanine-N(7)-)-methyltransferase subunit TRM82 OS=Pichia guilliermondii GN=TRM82 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 7.950e-12
Identity = 57/172 (33.14%), Postives = 88/172 (51.16%), Query Frame = -1

Query:    6 GNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGK-LFVSAGDDKTLKIWSTQ-----CQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDL--NQTSQDKKLTPTPLLSHYCSIITSLEFSPDNR-FIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCL 494
            G   VS +     G  P + + IRA+R  +  K LF +   DK + I++       C +        KR  A+++  + L V  ADKFG V+ + +  +Q   +K+LTP  +L H   +      S D + FII+ DRD  IR++N+PK+ +     I++F  GH EFVSCL
Sbjct:   71 GQAKVSKIPTPGPGAPPIY-NYIRALRLSSDEKYLFATTDSDKAVVIFTIDHSKDNCLELKKRQPFSKRPCALSVDEDRLVV--ADKFGDVYSIAIEDDQVVNEKELTP--ILGHVSMLSDVAIVSNDKKKFIITGDRDEHIRISNYPKSFV-----IKNFLFGHHEFVSCL 232

[Close]

BLAST of FG534552 vs. SwissProt
Match: WUHO_DROMO (tRNA (guanine-N(7)-)-methyltransferase subunit wuho OS=Drosophila mojavensis GN=wuho PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.362e-10
Identity = 41/100 (41.00%), Postives = 56/100 (56.00%), Query Frame = -1

Query:    3 TAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 302
            +A+A + +   V   DK G  +  D  +     KL    LL H  SI+  + ++PD + II+ DRD KIRVTN+P       H+I S+CLGH EFVS LA
Sbjct:  111 SALAFAPDSSSVLVTDKTGDCYQYDCVEVEAPPKL----LLGHL-SIVYDIVWTPDLKHIITCDRDDKIRVTNYP-----ATHDIHSYCLGHKEFVSGLA 200

[Close]

BLAST of FG534552 vs. TAIR peptide
Match: AT1G03110.1 (| Symbols: TRM82, AtTRM82 | Transducin/WD40 repeat-like superfamily protein | chr1:749359-751796 FORWARD LENGTH=427)

HSP 1 Score: 254.988 bits (650), Expect = 2.360e-68
Identity = 131/208 (62.98%), Postives = 160/208 (76.92%), Query Frame = -1

Query:    3 EEVAAETRKDTEVAPALIAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVDDSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTP---LLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSFCLGHTEFVSCLA 617
            EEV  E +   EVAPALI+VHP + S AVAVG +LR+F+L+ NC VSLVD+S   + P  K++IRAIR+   GKLFVSAGDDK +KIWS     C++TV SEKRV+AVAIS++  HVC+ADKFG+VWV++L+  +  K L       LLSHYCSIITSLEFSPD R+I+SADRDFKIRVT FPK  L GAHEIQSFCLGH+EF++C A
Sbjct:    8 EEV--EIQNKLEVAPALISVHPSQKSVAVAVGSDLRIFDLIENCPVSLVDES---DGPIRKESIRAIRYSTSGKLFVSAGDDKLVKIWSADSWRCLNTVCSEKRVSAVAISSDDSHVCYADKFGVVWVIELDGINDGKTLPSKKGALLLSHYCSIITSLEFSPDGRYILSADRDFKIRVTVFPKKPLEGAHEIQSFCLGHSEFITCTA 210

[Close]

BLAST of FG534552 vs. TAIR peptide
Match: AT3G18140.2 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr3:6213225-6214567 REVERSE LENGTH=224)

HSP 1 Score: 54.6842 bits (130), Expect = 4.681e-8
Identity = 44/177 (24.86%), Postives = 72/177 (40.68%), Query Frame = -1

Query:   39 IAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVD-DSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSF 566
            + + PD+H  A A  P +R+F++  N    ++  DS       H +N+ A+ F    K   S  +D T+KIW  +   C     S   V  V +      +   D+ G + V DL   S   +L P        + + SL    D   +++A+     R T +   LL G   +  F
Sbjct:   41 LEITPDKHYLAAACNPHIRLFDVNSNSPQPVMTYDS-------HTNNVMAVGFQCDAKWMYSGSEDGTVKIWDLRAPGCQKEYESVAAVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVP-----EVDTAVRSLTVMWDGTMVVAANN----RGTCYVWRLLRGKQTMTEF 201

[Close]

BLAST of FG534552 vs. TAIR peptide
Match: AT3G18140.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr3:6212743-6214567 REVERSE LENGTH=305)

HSP 1 Score: 54.6842 bits (130), Expect = 4.681e-8
Identity = 44/177 (24.86%), Postives = 72/177 (40.68%), Query Frame = -1

Query:   39 IAVHPDRHSAAVAVGPELRVFNLLGNCAVSLVD-DSVTGEQPFHKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTVSSEKRVTAVAISNEGLHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHEIQSF 566
            + + PD+H  A A  P +R+F++  N    ++  DS       H +N+ A+ F    K   S  +D T+KIW  +   C     S   V  V +      +   D+ G + V DL   S   +L P        + + SL    D   +++A+     R T +   LL G   +  F
Sbjct:   41 LEITPDKHYLAAACNPHIRLFDVNSNSPQPVMTYDS-------HTNNVMAVGFQCDAKWMYSGSEDGTVKIWDLRAPGCQKEYESVAAVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVP-----EVDTAVRSLTVMWDGTMVVAANN----RGTCYVWRLLRGKQTMTEF 201

[Close]

BLAST of FG534552 vs. TAIR peptide
Match: AT1G24130.1 (| Symbols: | Transducin/WD40 repeat-like superfamily protein | chr1:8534183-8535430 REVERSE LENGTH=415)

HSP 1 Score: 53.5286 bits (127), Expect = 1.043e-7
Identity = 41/154 (26.62%), Postives = 70/154 (45.45%), Query Frame = -1

Query:    3 HKDNIRAIRFGAKGKLFVSAGDDKTLKIWSTQCQDCVSTV--SSEKRVTAVAISNEG-LHVCFADKFGLVWVVDLNQTSQDKKLTPTPLLSHYCSIITSLEFSPDNRFIISADRDFKIRVTNFPKNLLNGAHE-----IQSFCLGHTEFVSCLA 440
            H D + ++     G L  SA  D++ KIW T    C+ ++  + +  + A+ +S +G ++   ADK   VW        +DKK +    L+ + S + +L  S D + + S   D  I V    + L+NG  E     +     GH + + CLA
Sbjct:  191 HVDAVSSLALSQDGSLLYSASWDRSFKIWRTSDFKCLDSIEKAHDDAINAIVVSKDGFVYTGSADKKIKVW------NKKDKKHSLVATLTKHLSAVNALAISEDGKVLYSGACDRSILVW---ERLINGDDEELHMSVVGALRGHRKAIMCLA 335

The following BLAST results are available for this feature:

BLAST of FG534552 vs. TrEMBL
BLAST of FG534552 vs. SwissProt
BLAST of FG534552 vs. TAIR peptide

BLAST of FG534552 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SFJ3_RICCO	3.802e-75	69.76	WD-repeat protein, putative OS=Ricinus communis GN...	[more]
B9H8A4_POPTR	5.490e-74	70.73	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TDY8_VITVI	2.819e-70	67.32	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7KCB5_ARALY	1.891e-66	64.42	Transducin family protein OS=Arabidopsis lyrata su...	[more]
Q93WD7_ARATH	3.226e-66	62.98	Putative uncharacterized protein At1g03110 OS=Arab...	[more]
Q94GP6_ORYSJ	6.960e-61	59.90	Os03g0746800 protein OS=Oryza sativa subsp. japoni...	[more]
B8AJV3_ORYSI	6.960e-61	59.90	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B6TK73_MAIZE	4.512e-60	60.71	WD-repeat protein 4 OS=Zea mays PE=2 SV=1	[more]
C5X165_SORBI	1.714e-59	60.20	Putative uncharacterized protein Sb01g008280 OS=So...	[more]
C0P5K9_MAIZE	3.819e-59	60.20	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of FG534552 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TRM82_YARLI	7.682e-15	29.72	tRNA (guanine-N(7)-)-methyltransferase subunit TRM...	[more]
WDR4_XENTR	1.109e-13	35.81	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_DICDI	1.109e-13	26.79	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_BOVIN	1.449e-13	35.46	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_MOUSE	5.506e-13	35.46	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_DANRE	7.190e-13	35.22	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_HUMAN	2.092e-12	36.69	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
WDR4_XENLA	3.569e-12	30.96	tRNA (guanine-N(7)-)-methyltransferase subunit WDR...	[more]
TRM82_PICGU	7.950e-12	33.14	tRNA (guanine-N(7)-)-methyltransferase subunit TRM...	[more]
WUHO_DROMO	4.362e-10	41.00	tRNA (guanine-N(7)-)-methyltransferase subunit wuh...	[more]

BLAST of FG534552 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G03110.1	2.360e-68	62.98	\| Symbols: TRM82, AtTRM82 \| Transducin/WD40 repeat...	[more]
AT3G18140.2	4.681e-8	24.86	\| Symbols: \| Transducin/WD40 repeat-like superfam...	[more]
AT3G18140.1	4.681e-8	24.86	\| Symbols: \| Transducin/WD40 repeat-like superfam...	[more]
AT1G24130.1	1.043e-7	26.62	\| Symbols: \| Transducin/WD40 repeat-like superfam...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	no description	coord: 51..90 score: 6.9e-05coord: 93..131 score: 3.6e+02coord: 140..179 score:
IPR011046	WD40 repeat-like-containing domain	SUPERFAMILY	50978	WD40 repeat-like	coord: 19..206 score: 4.9
IPR015943	WD40/YVTN repeat-like-containing domain	GENE3D	2.130.10.10	no description	coord: 20..205 score: 4
IPR017986	WD40-repeat-containing domain	PROFILE	PS50294	WD_REPEATS_REGION	coord: 58..99 score: 11
IPR019781	WD40 repeat, subgroup	PFAM	PF00400	WD40	coord: 61..90 score: 2.5e-06coord: 152..177 score: 0.0
IPR019782	WD40 repeat 2	PROFILE	PS50082	WD_REPEATS_2	coord: 58..99 score: 12
None	No IPR available	PANTHER	PTHR16288	WD40 REPEAT PROTEIN 4	coord: 87..195 score: 4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG534552 ID=FG534552; Name=FG534552; organism=Pisum sativum; type=EST; length=620bp
AAAGCAAGGCAGGAAACAAACTCTGTATGACCGAGACAAAAACTCTGAAT
CTCGTGAGCTCCATTTAAGAGATTCTTGGGAAAATTAGTAACACGAATTT
TGAAATCCCTATCAGCACTGATAATAAACCGATTATCTGGCGAAAACTCC
AAGCTAGTGATGATACTGCAATAATGAGAAAGAAGCGGTGTCGGTGTTAG
TTTCTTATCCTGCGAAGTTTGATTAAGATCCACAACCCAAACCAGACCGA
ATTTATCAGCGAAACACACGTGCAATCCTTCGTTGCTTATAGCAACAGCT
GTAACTCTCTTCTCCGACGACACAGTGGAAACGCAATCCTGGCATTGCGT
TGACCAAATTTTTAGGGTTTTATCATCACCAGCGGATACGAACAGTTTTC
CTTTAGCTCCGAATCGAATGGCTCTAATATTGTCTTTATGAAAGGGTTGT
TCACCGGTGACGGAGTCGTCAACTAAAGAAACTGCACAATTGCCAAGGAG
GTTGAAGACGCGGAGCTCCGGTCCGACTGCTACGGCGGCGGAGTGTCGGT
CTGGGTGAACGGCGATGAGTGCCGGCGCTACTTCAGTGTCTTTTCTGGTT
TCGGCGGCAACTTCTTCGTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR001680	WD40_rpt
IPR011046	WD40 repeat-like-containing domain
IPR015943	WD40/YVTN_repeat-like_dom_sf
IPR017986	WD40_repeat_dom
IPR019781	WD40 repeat, subgroup
IPR019782	WD40 repeat 2

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