FG537856

EST Overview


Name	FG537856
Unique Name	FG537856
Type	EST
Organism	Pisum sativum (pea)
Sequence length	671

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG537856 vs. TrEMBL
Match: D7TJ38_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033805001 PE=4 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.709e-51
Identity = 107/180 (59.44%), Postives = 127/180 (70.56%), Query Frame = 3

Query:    3 AGNQKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGSPNLK 542
            +GNQKPSSL   Q +  Q G  SR  +A    +  E G  +L KRSIHELVNQ+DP EKLDPEV DIL DIAE+F+ESI   GCSLAKHRKS TLEAKD+LLHLE+NWN+TLPGFGGDEIK ++KP  +DIHKER+AAIKKS V TE+A+ +                T  +V+ SPNLK
Sbjct:  192 SGNQKPSSLAIVQPSTPQLGPHSRTASA----EASESGNRILSKRSIHELVNQIDPSEKLDPEVEDILVDIAEDFVESITTFGCSLAKHRKSPTLEAKDILLHLERNWNMTLPGFGGDEIKTFKKPFVSDIHKERLAAIKKSAVGTESANTKSSSGQGAGNTKGHPAKTSANVLSSPNLK 367

[Close]

BLAST of FG537856 vs. TrEMBL
Match: B9SRU6_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0515670 PE=4 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 4.782e-48
Identity = 104/186 (55.91%), Postives = 127/186 (68.28%), Query Frame = 3

Query:    9 NQKPSSLVAAQ--------TNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGSPNLK 542
            NQKPSSL            +N VQPG+ +R   A+P +D  E G  +L KR+IHELV Q+DP EKLDPEV DIL DIA+ F+ESI   GCSLAKHRKS TLEAKD+LLHLE+NWN+TL GF GDEIK YRKPL++DIHKER+A IKKS++A++ A  +                TP + + SPNLK
Sbjct:  269 NQKPSSLAVVHPSPVANTVSNTVQPGTLNR--TAIPESD--ESGNRILSKRNIHELVTQIDPSEKLDPEVEDILADIADEFVESITTFGCSLAKHRKSDTLEAKDILLHLERNWNMTLTGFSGDEIKTYRKPLTSDIHKERLAVIKKSILASDMASAKSSVGQAAGNAKSNLTRTPANAMISPNLK 450

[Close]

BLAST of FG537856 vs. TrEMBL
Match: B9HBK0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_560743 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 1.178e-46
Identity = 98/178 (55.06%), Postives = 124/178 (69.66%), Query Frame = 3

Query:    9 NQKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGSPNLK 542
            N KPSSL  +  N VQP  Q    N++ NA+ +E G  +L KRSIHELV+Q+DP EK +PEV +IL DIA+ FL S+   GCSLAKHRKS TLEAKD+LLHL++NWN+TLPGF GDEIK+YRK ++ DIHKER+AAIKKS++ +E A+ +                T  + I SPNLK
Sbjct:  322 NHKPSSLAMSHPNTVQPLPQ----NSIANAESDESGNRILSKRSIHELVSQIDPSEKFNPEVVEILADIADEFLVSVTTFGCSLAKHRKSDTLEAKDILLHLDRNWNMTLPGFCGDEIKSYRKQVTNDIHKERLAAIKKSILTSEMANAKNSVGQAAGNAKSSTTKTLSNPIVSPNLK 495

[Close]

BLAST of FG537856 vs. TrEMBL
Match: D7L8V2_ARALY (Tata-associated factor II 58 OS=Arabidopsis lyrata subsp. lyrata GN=TAFII58 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 3.207e-44
Identity = 97/175 (55.43%), Postives = 120/175 (68.57%), Query Frame = 3

Query:    9 NQKPSS-LVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGS 530
            NQK +S ++ +Q    QPG+ ++  +A    + E     +LGKRSIHEL+ Q+DP EKLDPEV DIL DIAE+F+ESI   GCSLAKHRKS TLEAKD+LLH+E+NWNI  PGF  DE K +RKPL+TDIHKER+AAIKKS+ ATEAA  R                TP + +GS
Sbjct:  366 NQKVTSPVMPSQPPVAQPGNHAKTVSA----ENETSDDRILGKRSIHELLQQIDPSEKLDPEVEDILADIAEDFVESITTFGCSLAKHRKSDTLEAKDILLHVERNWNIRPPGFSSDEFKTFRKPLTTDIHKERLAAIKKSVTATEAASARNQFGHGTANARGGQSKTPSNPLGS 536

[Close]

BLAST of FG537856 vs. TrEMBL
Match: Q9SR71_ARATH (Putative TBP-associated 58 kDa subunit protein OS=Arabidopsis thaliana GN=TAF12 PE=2 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 9.332e-44
Identity = 96/175 (54.86%), Postives = 120/175 (68.57%), Query Frame = 3

Query:    9 NQKPSS-LVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGS 530
            NQK +S ++ +Q    QPG+ ++  +A    + E     +LGKRSIHEL+ Q+DP EKLDPEV DIL DIAE+F+ESI   GCSLAKHRKS  LEAKD+LLH+E+NWNI  PGF  DE K +RKPL+TDIHKER+AAIKKS+ ATEAA+ R                TP + +GS
Sbjct:  364 NQKVTSPVMPSQPPVAQPGNHAKTVSA----ETEPSDDRILGKRSIHELLQQIDPSEKLDPEVEDILSDIAEDFVESITTFGCSLAKHRKSDILEAKDILLHVERNWNIRPPGFSSDEFKTFRKPLTTDIHKERLAAIKKSVTATEAANARNQFGHGTANARGGQAKTPSNPMGS 534

[Close]

BLAST of FG537856 vs. TrEMBL
Match: Q5N902_ORYSJ (Transcription initiation factor IID (TFIID) subunit A-like protein OS=Oryza sativa subsp. japonica GN=P0683F02.25 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.461e-36
Identity = 82/146 (56.16%), Postives = 99/146 (67.81%), Query Frame = 3

Query:   12 QKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAA 449
            QK  S    +   VQ G  + +     + D    G  +L KRSIHELV Q+DP EKLDPEV D+L DIAE+F+ES+    CSLAKHRKS+ LEAKDVLLH E++WNITLPGF GDEIK Y+KP   DIH+ER+  IKKSM +   A
Sbjct:  131 QKLDSPAVLKATNVQSGDMASV-----DVDAGGSGNRLLSKRSIHELVAQIDPSEKLDPEVEDVLIDIAEDFVESVATFACSLAKHRKSSILEAKDVLLHAERSWNITLPGFSGDEIKLYKKPHVNDIHRERLTLIKKSMASESNA 271

[Close]

BLAST of FG537856 vs. TrEMBL
Match: Q0JHK4_ORYSJ (Os01g0858500 protein OS=Oryza sativa subsp. japonica GN=Os01g0858500 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.461e-36
Identity = 82/146 (56.16%), Postives = 99/146 (67.81%), Query Frame = 3

Query:   12 QKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAA 449
            QK  S    +   VQ G  + +     + D    G  +L KRSIHELV Q+DP EKLDPEV D+L DIAE+F+ES+    CSLAKHRKS+ LEAKDVLLH E++WNITLPGF GDEIK Y+KP   DIH+ER+  IKKSM +   A
Sbjct:  137 QKLDSPAVLKATNVQSGDMASV-----DVDAGGSGNRLLSKRSIHELVAQIDPSEKLDPEVEDVLIDIAEDFVESVATFACSLAKHRKSSILEAKDVLLHAERSWNITLPGFSGDEIKLYKKPHVNDIHRERLTLIKKSMASESNA 277

[Close]

BLAST of FG537856 vs. TrEMBL
Match: B8ACH7_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04504 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.461e-36
Identity = 82/146 (56.16%), Postives = 99/146 (67.81%), Query Frame = 3

Query:   12 QKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAA 449
            QK  S    +   VQ G  + +     + D    G  +L KRSIHELV Q+DP EKLDPEV D+L DIAE+F+ES+    CSLAKHRKS+ LEAKDVLLH E++WNITLPGF GDEIK Y+KP   DIH+ER+  IKKSM +   A
Sbjct:  330 QKLDSPAVLKATNVQSGDMASV-----DVDAGGSGNRLLSKRSIHELVAQIDPSEKLDPEVEDVLIDIAEDFVESVATFACSLAKHRKSSILEAKDVLLHAERSWNITLPGFSGDEIKLYKKPHVNDIHRERLTLIKKSMASESNA 470

[Close]

BLAST of FG537856 vs. TrEMBL
Match: C5XQH8_SORBI (Putative uncharacterized protein Sb03g040460 OS=Sorghum bicolor GN=Sb03g040460 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 3.215e-36
Identity = 77/116 (66.38%), Postives = 92/116 (79.31%), Query Frame = 3

Query:   84 ALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSM 431
            A  +A+  E G  ++ KRSIHELV Q+DP EKLDPEV D+L DIAE+F+ES+    CSLAKHRKS+TLEAKDVLLH E++WNITLPGF GDEIK Y+K    DIH+ER+A IKKSM
Sbjct:  355 AATDANGSETGARLITKRSIHELVAQIDPNEKLDPEVEDVLIDIAEDFVESVTTFACSLAKHRKSSTLEAKDVLLHAERSWNITLPGFSGDEIKLYKKQHINDIHRERLALIKKSM 470

[Close]

BLAST of FG537856 vs. TrEMBL
Match: A9TS71_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_27365 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.545e-30
Identity = 60/102 (58.82%), Postives = 84/102 (82.35%), Query Frame = 3

Query:  123 VLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKS 428
            +L KRSI +LV Q+DP E+LDP+V D+L +IA++F+ES+    C LAKHRKS  LEAKDVLLHL+K WNIT+PGFGG+EI++Y++P  ++ HK+R+A ++KS
Sbjct:    1 ILRKRSIQDLVAQIDPKERLDPDVEDVLLEIADDFIESVASFACKLAKHRKSAVLEAKDVLLHLDKQWNITVPGFGGEEIRSYKRPQISESHKQRLAVVRKS 102

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BLAST of FG537856 vs. SwissProt
Match: TAF12_XENLA (Transcription initiation factor TFIID subunit 12 OS=Xenopus laevis GN=taf12 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.427e-28
Identity = 57/111 (51.35%), Postives = 83/111 (74.77%), Query Frame = 3

Query:   96 ADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKS 428
            A PE     VL K+ +H+LV +VDP E+LD +V ++L  IA++F+ES++ + C LA+HRKS TLE KDV LHLE+ WN+ +PGFG +EI+ Y+K  +T+ HK+R+A IKK+
Sbjct:   53 ASPE--ANQVLSKKKLHDLVREVDPNEQLDEDVEEMLLQIADDFIESVVSAACQLARHRKSNTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIKKT 161

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BLAST of FG537856 vs. SwissProt
Match: TAF12_DICDI (Transcription initiation factor TFIID subunit 12 OS=Dictyostelium discoideum GN=TAF12 PE=3 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 7.061e-28
Identity = 63/130 (48.46%), Postives = 88/130 (67.69%), Query Frame = 3

Query:   63 SQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGG-DEIKNYRKPLSTDIHKERIAAIKKSMVATEAA 449
            + S  PN   N +PE     VLGKR + EL+ Q+ P EK+D +  DIL  +A++F+ES +   C+LAKHR STTLE KD+  HLEKNWNI +PGFG  ++ K ++KP   + HK R+AA+KKS+  T+A+
Sbjct:  426 NNSNNPNNPNNIEPE-----VLGKRKLIELLQQISPNEKMDEDAEDILSVLADDFVESTVAFACTLAKHRNSTTLEVKDLQCHLEKNWNIRVPGFGNVEQYKTFKKPHFPENHKLRVAAMKKSIALTQAS 550

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BLAST of FG537856 vs. SwissProt
Match: TAF12_HUMAN (Transcription initiation factor TFIID subunit 12 OS=Homo sapiens GN=TAF12 PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.683e-27
Identity = 62/138 (44.93%), Postives = 93/138 (67.39%), Query Frame = 3

Query:   18 PSSLVAAQTNAVQ-PGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKS 428
            P   +A  T  V+ PG+    P A     PE     VL K+ + +LV +VDP E+LD +V ++L  IA++F+ES++ + C LA+HRKS+TLE KDV LHLE+ WN+ +PGFG +EI+ Y+K  +T+ HK+R+A I+K+
Sbjct:   27 PQGSMANSTAVVKIPGT----PGAGGRLSPEN--NQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKT 158

[Close]

BLAST of FG537856 vs. SwissProt
Match: TAF12_MOUSE (Transcription initiation factor TFIID subunit 12 OS=Mus musculus GN=Taf12 PE=1 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 3.504e-27
Identity = 57/131 (43.51%), Postives = 88/131 (67.18%), Query Frame = 3

Query:   36 AQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKS 428
            +  N+   G  +  P       PE     VL K+ + +LV +VDP E+LD +V ++L  IA++F+ES++ + C LA+HRKS+TLE KDV LHLE+ WN+ +PGFG +EI+ Y+K  +T+ HK+R+A I+K+
Sbjct:   30 SMANSTTVGKIAGTPGTGGRLSPEN--NQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKT 158

[Close]

BLAST of FG537856 vs. SwissProt
Match: TAF12_BOVIN (Transcription initiation factor TFIID subunit 12 OS=Bos taurus GN=TAF12 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 5.977e-27
Identity = 62/141 (43.97%), Postives = 93/141 (65.96%), Query Frame = 3

Query:   18 PSSLVAAQTNAVQ----PGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKS 428
            P   +A  T  V+    PG+  RL        PE     VL K+ + +LV +VDP E+LD +V ++L  IA++F+ES++ + C LA+HRKS+TLE KDV LHLE+ WN+ +PGFG +EI+ Y+K  +T+ HK+R+A I+K+
Sbjct:   27 PQGSMANSTAVVKIPGTPGTGGRL-------SPEN--NQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKT 158

[Close]

BLAST of FG537856 vs. SwissProt
Match: TAF12_SCHPO (Transcription initiation factor TFIID subunit 12 OS=Schizosaccharomyces pombe GN=taf12 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.167e-22
Identity = 59/132 (44.70%), Postives = 81/132 (61.36%), Query Frame = 3

Query:   33 AAQTNAVQPGSQSRLPNALPNADPEEL--GKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEI-KNYRKPLSTDIHKERIAAI 419
            A  T     GS       L  A   EL  G  +L KR +H+L+ Q+D  EK++PEV ++L +IA+ F+ES+    C LAKHRKS TL+ +DV LHLE+NWNI LPGF  D+I K+ RK   T  ++++  AI
Sbjct:  310 ATLTGGYASGSIGLSTPGLSRAPHYELDNGNRLLSKRKLHDLLQQIDSEEKIEPEVEELLLEIADEFVESVTNFACRLAKHRKSDTLDVRDVQLHLERNWNIRLPGFASDDIVKSARKTGPTPSYQQKQNAI 441

[Close]

BLAST of FG537856 vs. SwissProt
Match: TAF12_DROME (Transcription initiation factor TFIID subunit 12 OS=Drosophila melanogaster GN=Taf12 PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.577e-19
Identity = 52/141 (36.88%), Postives = 85/141 (60.28%), Query Frame = 3

Query:    9 NQKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSM 431
            N  P S +   T  +  GS+S   N   +A   E    +L K  + ELV +VD   +LD +V ++L  I ++F+E  ++S  + AKHRKS  +E +DV LH E+ +N+ +PGFG DE++ Y++   T+ HK+R+A I+K++
Sbjct:   56 NGGPVSGLGTGTGPISGGSKSS--NHTSSAAGSE-NTPMLTKPRLTELVREVDTTTQLDEDVEELLLQIIDDFVEDTVKSTSAFAKHRKSNKIEVRDVQLHFERKYNMWIPGFGTDELRPYKRAAVTEAHKQRLALIRKTI 193

[Close]

BLAST of FG537856 vs. SwissProt
Match: TAF12_YEAST (Transcription initiation factor TFIID subunit 12 OS=Saccharomyces cerevisiae GN=TAF12 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.287e-17
Identity = 50/141 (35.46%), Postives = 82/141 (58.16%), Query Frame = 3

Query:   33 AAQTNAVQPGSQSRLPNALPNADPEELG-KSVLGKRSIHELVNQV-----DPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVA 437
            A   +A+   + ++LP       P E+  + V+ KR + ELV  V     D    +D +V ++L D+A++F+ ++    C LAKHRKS  LEA+D+ LHLE+NWNI +PG+  DEI++ RK   +  + +++ +I    VA
Sbjct:  390 AMNASALNTPATTKLP-------PYEMDTQRVMSKRKLRELVKTVGIDEGDGETVIDGDVEELLLDLADDFVTNVTAFSCRLAKHRKSDNLEARDIQLHLERNWNIRIPGYSADEIRSTRKWNPSQNYNQKLQSITSDKVA 523

[Close]

BLAST of FG537856 vs. TAIR peptide
Match: AT3G10070.1 (| Symbols: TAF12, TAFII58 | TBP-associated factor 12 | chr3:3104195-3106798 REVERSE LENGTH=539)

HSP 1 Score: 180.644 bits (457), Expect = 6.458e-46
Identity = 96/175 (54.86%), Postives = 120/175 (68.57%), Query Frame = 3

Query:    9 NQKPSS-LVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIKKSMVATEAAHPRXXXXXXXXXXXXXXXXTPFSVIGS 530
            NQK +S ++ +Q    QPG+ ++  +A    + E     +LGKRSIHEL+ Q+DP EKLDPEV DIL DIAE+F+ESI   GCSLAKHRKS  LEAKD+LLH+E+NWNI  PGF  DE K +RKPL+TDIHKER+AAIKKS+ ATEAA+ R                TP + +GS
Sbjct:  364 NQKVTSPVMPSQPPVAQPGNHAKTVSA----ETEPSDDRILGKRSIHELLQQIDPSEKLDPEVEDILSDIAEDFVESITTFGCSLAKHRKSDILEAKDILLHVERNWNIRPPGFSSDEFKTFRKPLTTDIHKERLAAIKKSVTATEAANARNQFGHGTANARGGQAKTPSNPMGS 534

[Close]

BLAST of FG537856 vs. TAIR peptide
Match: AT1G17440.2 (| Symbols: EER4, TAF12B | Transcription initiation factor TFIID subunit A | chr1:5984407-5987663 REVERSE LENGTH=683)

HSP 1 Score: 119.013 bits (297), Expect = 2.308e-27
Identity = 62/137 (45.26%), Postives = 92/137 (67.15%), Query Frame = 3

Query:   12 QKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIK 422
            QK  SL  +Q  A Q G+ +  P    ++   E    +LGKR I +LV+QVD   KLDP+V D+L ++A++F++S+    CSLAKHRKS+ LE KD+LLHLEKN ++T+PGF  ++ K   K + TD+HK+R+A ++
Sbjct:  493 QKSVSLTGSQPEATQSGTTT--PGG-SSSQGTEATNQLLGKRKIQDLVSQVDVHAKLDPDVEDLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLHLTIPGFSSED-KRQTKTVPTDLHKKRLAMVR 625

[Close]

BLAST of FG537856 vs. TAIR peptide
Match: AT1G17440.1 (| Symbols: EER4, TAF12B | Transcription initiation factor TFIID subunit A | chr1:5984407-5987663 REVERSE LENGTH=683)

HSP 1 Score: 119.013 bits (297), Expect = 2.308e-27
Identity = 62/137 (45.26%), Postives = 92/137 (67.15%), Query Frame = 3

Query:   12 QKPSSLVAAQTNAVQPGSQSRLPNALPNADPEELGKSVLGKRSIHELVNQVDPLEKLDPEVADILGDIAENFLESIIRSGCSLAKHRKSTTLEAKDVLLHLEKNWNITLPGFGGDEIKNYRKPLSTDIHKERIAAIK 422
            QK  SL  +Q  A Q G+ +  P    ++   E    +LGKR I +LV+QVD   KLDP+V D+L ++A++F++S+    CSLAKHRKS+ LE KD+LLHLEKN ++T+PGF  ++ K   K + TD+HK+R+A ++
Sbjct:  493 QKSVSLTGSQPEATQSGTTT--PGG-SSSQGTEATNQLLGKRKIQDLVSQVDVHAKLDPDVEDLLLEVADDFIDSVTSFACSLAKHRKSSVLEPKDILLHLEKNLHLTIPGFSSED-KRQTKTVPTDLHKKRLAMVR 625

The following BLAST results are available for this feature:

BLAST of FG537856 vs. TrEMBL
BLAST of FG537856 vs. SwissProt
BLAST of FG537856 vs. TAIR peptide

BLAST of FG537856 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TJ38_VITVI	2.709e-51	59.44	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SRU6_RICCO	4.782e-48	55.91	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9HBK0_POPTR	1.178e-46	55.06	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7L8V2_ARALY	3.207e-44	55.43	Tata-associated factor II 58 OS=Arabidopsis lyrata...	[more]
Q9SR71_ARATH	9.332e-44	54.86	Putative TBP-associated 58 kDa subunit protein OS=...	[more]
Q5N902_ORYSJ	2.461e-36	56.16	Transcription initiation factor IID (TFIID) subuni...	[more]
Q0JHK4_ORYSJ	2.461e-36	56.16	Os01g0858500 protein OS=Oryza sativa subsp. japoni...	[more]
B8ACH7_ORYSI	2.461e-36	56.16	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5XQH8_SORBI	3.215e-36	66.38	Putative uncharacterized protein Sb03g040460 OS=So...	[more]
A9TS71_PHYPA	1.545e-30	58.82	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]

BLAST of FG537856 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
TAF12_XENLA	2.427e-28	51.35	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_DICDI	7.061e-28	48.46	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_HUMAN	2.683e-27	44.93	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_MOUSE	3.504e-27	43.51	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_BOVIN	5.977e-27	43.97	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_SCHPO	1.167e-22	44.70	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_DROME	1.577e-19	36.88	Transcription initiation factor TFIID subunit 12 O...	[more]
TAF12_YEAST	3.287e-17	35.46	Transcription initiation factor TFIID subunit 12 O...	[more]

BLAST of FG537856 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G10070.1	6.458e-46	54.86	\| Symbols: TAF12, TAFII58 \| TBP-associated factor ...	[more]
AT1G17440.2	2.308e-27	45.26	\| Symbols: EER4, TAF12B \| Transcription initiation...	[more]
AT1G17440.1	2.308e-27	45.26	\| Symbols: EER4, TAF12B \| Transcription initiation...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 2..30 score
None	No IPR available	PANTHER	PTHR12264	FAMILY NOT NAMED	coord: 41..134 score: 3.5
None	No IPR available	PANTHER	PTHR12264:SF4	gb def: T22K18.10 protein (TAF12) (Putative TBP-associated 58 kDa subunit protein)	coord: 41..134 score: 3.5
None	No IPR available	SEG	seg	seg	coord: 153..168 score
IPR003228	Transcription initiation factor TFIID	PFAM	PF03847	TFIID_20kDa	coord: 43..110 score: 1.4
IPR009072	Histone-fold	GENE3D	1.10.20.10	no description	coord: 39..111 score: 3.6
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 39..112 score: 2.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG537856 ID=FG537856; Name=FG537856; organism=Pisum sativum; type=EST; length=671bp
GGGCAGGAAATCAGAAACCTTCATCACTTGTGGCTGCACAAACTAATGCA
GTTCAACCAGGGAGTCAAAGTAGATTACCAAATGCTTTACCAAATGCAGA
TCCAGAAGAGTTGGGTAAGAGTGTTCTCGGAAAAAGAAGCATCCATGAGC
TAGTCAATCAGGTTGATCCGTTGGAGAAGTTAGATCCTGAAGTTGCAGAC
ATTCTTGGCGATATTGCAGAAAATTTTCTGGAGTCTATAATTAGGTCTGG
TTGCTCACTAGCGAAGCATCGAAAATCAACAACTCTGGAAGCCAAGGACG
TACTCTTACATCTTGAGAAAAATTGGAATATTACACTTCCTGGATTTGGT
GGCGATGAGATTAAAAACTACAGAAAACCACTTTCAACTGATATTCACAA
GGAGCGCATAGCGGCTATAAAGAAATCAATGGTAGCAACTGAGGCTGCAC
ATCCTAGGGGCTCTGCTGGTCAGGCTTCTGGTAGTGCAAAGGGTAGTCAG
GCAAAGACACCTTTCAGTGTCATTGGCTCTCCCAACCTTAAAAATTCATA
AGGTGGACGCGTTTGCGCTTTATACAGCAATTGAGAAACTGCTTTGGTGA
AGGTAGTTCTGTTTGCAATTGTATTGTAAACATAAATGATATCAACATCC
ATTATTTAATCGGCCACATGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005669	transcription factor TFIID complex

Vocabulary: **Biological Process**
Term	Definition
GO:0006352	DNA-templated transcription initiation

Vocabulary: **Molecular Function**
Term	Definition
GO:0016986	obsolete transcription initiation factor activity
GO:0003677	DNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR003228	TFIID_TAF12_dom
IPR009072	Histone-fold

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