GH719223

EST Overview


Name	GH719223
Unique Name	GH719223
Type	EST
Organism	Pisum sativum (pea)
Sequence length	262

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719223 vs. TrEMBL
Match: C6T3K9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.337e-20
Identity = 45/50 (90.00%), Postives = 50/50 (100.00%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPDDS 152
            RLIGSDIEHYIRK+LYDGVIKYNMNARVLNFSMGKP+IMFNNND+QP+D+
Sbjct:  166 RLIGSDIEHYIRKMLYDGVIKYNMNARVLNFSMGKPKIMFNNNDIQPEDA 215

[Close]

BLAST of GH719223 vs. TrEMBL
Match: B9S2T3_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0561560 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.799e-16
Identity = 42/48 (87.50%), Postives = 43/48 (89.58%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPD 146
            RLIGSDIEHYIRKLLYDG IKYNMNA VLNFSMGKPRIMFNN+D   D
Sbjct:  160 RLIGSDIEHYIRKLLYDGEIKYNMNANVLNFSMGKPRIMFNNDDELQD 207

[Close]

BLAST of GH719223 vs. TrEMBL
Match: B9H7J7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_714597 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.444e-15
Identity = 40/43 (93.02%), Postives = 42/43 (97.67%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNN 131
            RLIGSDIEHYIRKLLYDG IKYNMNARVLNFSMGKPRI+FNN+
Sbjct:   81 RLIGSDIEHYIRKLLYDGEIKYNMNARVLNFSMGKPRILFNND 123

[Close]

BLAST of GH719223 vs. TrEMBL
Match: B6TYZ1_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.486e-15
Identity = 38/45 (84.44%), Postives = 42/45 (93.33%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDV 137
            RLIGSD+EHYIRKLLYDGVIKYNM +RVLNFSMGKPRI FN+N +
Sbjct:  162 RLIGSDLEHYIRKLLYDGVIKYNMRSRVLNFSMGKPRIKFNSNQI 206

[Close]

BLAST of GH719223 vs. TrEMBL
Match: B4FRX4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.486e-15
Identity = 38/45 (84.44%), Postives = 42/45 (93.33%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDV 137
            RLIGSD+EHYIRKLLYDGVIKYNM +RVLNFSMGKPRI FN+N +
Sbjct:  162 RLIGSDLEHYIRKLLYDGVIKYNMRSRVLNFSMGKPRIKFNSNQI 206

[Close]

BLAST of GH719223 vs. TrEMBL
Match: C9EIN8_OLEEU (Putative NADH dehydrogenase OS=Olea europaea PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.222e-14
Identity = 40/49 (81.63%), Postives = 43/49 (87.76%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPDD 149
            RLIGSDIEHYIRKLLY+G I+YNMNAR+LNFSMGKPRI FN ND Q  D
Sbjct:  157 RLIGSDIEHYIRKLLYEGEIQYNMNARMLNFSMGKPRIGFNYNDGQIQD 205

[Close]

BLAST of GH719223 vs. TrEMBL
Match: C5YD00_SORBI (Putative uncharacterized protein Sb06g023900 OS=Sorghum bicolor GN=Sb06g023900 PE=4 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.556e-14
Identity = 35/45 (77.78%), Postives = 42/45 (93.33%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDV 137
            RLIGSD+EHYIRK+LYDGV+KYNM +RVLNFSMGKPR+ FN+N +
Sbjct:  170 RLIGSDLEHYIRKMLYDGVLKYNMRSRVLNFSMGKPRMKFNSNQI 214

[Close]

BLAST of GH719223 vs. TrEMBL
Match: D7M9M2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_327981 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.305e-13
Identity = 33/46 (71.74%), Postives = 43/46 (93.48%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQ 140
            RLIGSD+EHYIRK+L+DG I+YNM+ARVLNFSMGKPR+ FN ++++
Sbjct:  164 RLIGSDVEHYIRKMLFDGEIQYNMDARVLNFSMGKPRVQFNTSNIE 209

[Close]

BLAST of GH719223 vs. TrEMBL
Match: B7EXV4_ORYSJ (cDNA clone:001-118-H01, full insert sequence OS=Oryza sativa subsp. japonica PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.932e-13
Identity = 37/48 (77.08%), Postives = 43/48 (89.58%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPD 146
            RLIGSD+EH+IRKLLYDG IKYNM +RVLNFSMGKPRI FN++ + PD
Sbjct:  172 RLIGSDLEHFIRKLLYDGEIKYNMMSRVLNFSMGKPRIKFNSSQI-PD 218

[Close]

BLAST of GH719223 vs. TrEMBL
Match: A0ZLJ3_NODSP (NADH dehydrogenase I subunit M OS=Nodularia spumigena CCY9414 GN=N9414_00590 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.211e-7
Identity = 25/43 (58.14%), Postives = 33/43 (76.74%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNN 131
            R IGSD+EHY+R LL  G I YN++ARV N+SMG P+++ N N
Sbjct:   72 RRIGSDLEHYLRSLLQKGEISYNLSARVTNYSMGLPQVVANEN 114

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_POPJC (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Populus jackii GN=ndhM PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.192e-17
Identity = 40/43 (93.02%), Postives = 42/43 (97.67%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNN 131
            RLIGSDIEHYIRKLLYDG IKYNMNARVLNFSMGKPRI+FNN+
Sbjct:  155 RLIGSDIEHYIRKLLYDGEIKYNMNARVLNFSMGKPRILFNND 197

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_VITVI (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Vitis vinifera GN=ndhM PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.570e-16
Identity = 39/42 (92.86%), Postives = 41/42 (97.62%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNN 128
            RLIGSDIEHYIRKLLYDG IKYNMNARVLNFSMGKPRI+FN+
Sbjct:  160 RLIGSDIEHYIRKLLYDGEIKYNMNARVLNFSMGKPRILFND 201

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_ARATH (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Arabidopsis thaliana GN=ndhM PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.148e-14
Identity = 33/46 (71.74%), Postives = 43/46 (93.48%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQ 140
            RLIGSD+EHYIRK+L+DG I+YNM+ARVLNFSMGKPR+ FN ++++
Sbjct:  161 RLIGSDVEHYIRKMLFDGEIQYNMDARVLNFSMGKPRVQFNTSNIE 206

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_ORYSJ (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. japonica GN=ndhM PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.959e-14
Identity = 37/48 (77.08%), Postives = 43/48 (89.58%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPD 146
            RLIGSD+EH+IRKLLYDG IKYNM +RVLNFSMGKPRI FN++ + PD
Sbjct:  172 RLIGSDLEHFIRKLLYDGEIKYNMMSRVLNFSMGKPRIKFNSSQI-PD 218

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_ORYSI (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Oryza sativa subsp. indica GN=ndhM PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.959e-14
Identity = 37/48 (77.08%), Postives = 43/48 (89.58%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQPD 146
            RLIGSD+EH+IRKLLYDG IKYNM +RVLNFSMGKPRI FN++ + PD
Sbjct:  172 RLIGSDLEHFIRKLLYDGEIKYNMMSRVLNFSMGKPRIKFNSSQI-PD 218

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_PHYPA (NAD(P)H-quinone oxidoreductase subunit M, chloroplastic OS=Physcomitrella patens GN=ndhM PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.006e-10
Identity = 30/38 (78.95%), Postives = 34/38 (89.47%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRI 116
            RLIGSD+EHYIRKLL  G +KYN++ RVLNFSMGKPRI
Sbjct:  155 RLIGSDLEHYIRKLLLAGELKYNLDCRVLNFSMGKPRI 192

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_ACAM1 (NAD(P)H-quinone oxidoreductase subunit M OS=Acaryochloris marina (strain MBIC 11017) GN=ndhM PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.776e-7
Identity = 22/39 (56.41%), Postives = 32/39 (82.05%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIM 119
            R IGSD+EHY+R LL  G++ YN+++RV NFSMG P+++
Sbjct:   71 RRIGSDLEHYVRSLLQKGIVGYNLSSRVRNFSMGLPQVI 109

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_MICAN (NAD(P)H-quinone oxidoreductase subunit M OS=Microcystis aeruginosa (strain NIES-843) GN=ndhM PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.319e-7
Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRI 116
            R IGSD+EH+IR LL  G I YN+NARV N+SMG P++
Sbjct:   71 RRIGSDLEHFIRSLLQKGEISYNLNARVQNYSMGLPKL 108

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_NOSP7 (NAD(P)H-quinone oxidoreductase subunit M OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=ndhM PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.029e-7
Identity = 23/45 (51.11%), Postives = 33/45 (73.33%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDV 137
            R +GSD+EHY+R LL  G I YN++ARV N+SMG P++  ++  V
Sbjct:   76 RRVGSDLEHYLRSLLQLGEISYNLSARVTNYSMGVPQVAIDDKQV 120

[Close]

BLAST of GH719223 vs. SwissProt
Match: NDHM_CYAA5 (NAD(P)H-quinone oxidoreductase subunit M OS=Cyanothece sp. (strain ATCC 51142) GN=ndhM PE=3 SV=2)

HSP 1 Score: 53.9138 bits (128), Expect = 3.029e-7
Identity = 22/38 (57.89%), Postives = 31/38 (81.58%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRI 116
            R IGSD+EH++R LL +G I YN+ +RVLN+SMG P++
Sbjct:   71 RRIGSDLEHFVRNLLQNGEISYNLKSRVLNYSMGLPKV 108

[Close]

BLAST of GH719223 vs. TAIR peptide
Match: AT4G37925.1 (| Symbols: NDH-M | subunit NDH-M of NAD(P)H:plastoquinone dehydrogenase complex | chr4:17830748-17831485 REVERSE LENGTH=217)

HSP 1 Score: 78.5666 bits (192), Expect = 8.974e-16
Identity = 33/46 (71.74%), Postives = 43/46 (93.48%), Query Frame = 3

Query:    3 RLIGSDIEHYIRKLLYDGVIKYNMNARVLNFSMGKPRIMFNNNDVQ 140
            RLIGSD+EHYIRK+L+DG I+YNM+ARVLNFSMGKPR+ FN ++++
Sbjct:  161 RLIGSDVEHYIRKMLFDGEIQYNMDARVLNFSMGKPRVQFNTSNIE 206

The following BLAST results are available for this feature:

BLAST of GH719223 vs. TrEMBL
BLAST of GH719223 vs. SwissProt
BLAST of GH719223 vs. TAIR peptide

BLAST of GH719223 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T3K9_SOYBN	4.337e-20	90.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9S2T3_RICCO	3.799e-16	87.50	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9H7J7_POPTR	1.444e-15	93.02	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B6TYZ1_MAIZE	5.486e-15	84.44	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FRX4_MAIZE	5.486e-15	84.44	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C9EIN8_OLEEU	1.222e-14	81.63	Putative NADH dehydrogenase OS=Olea europaea PE=2 ...	[more]
C5YD00_SORBI	3.556e-14	77.78	Putative uncharacterized protein Sb06g023900 OS=So...	[more]
D7M9M2_ARALY	2.305e-13	71.74	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B7EXV4_ORYSJ	3.932e-13	77.08	cDNA clone:001-118-H01, full insert sequence OS=Or...	[more]
A0ZLJ3_NODSP	4.211e-7	58.14	NADH dehydrogenase I subunit M OS=Nodularia spumig...	[more]

BLAST of GH719223 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NDHM_POPJC	7.192e-17	93.02	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_VITVI	3.570e-16	92.86	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_ARATH	1.148e-14	71.74	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_ORYSJ	1.959e-14	77.08	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_ORYSI	1.959e-14	77.08	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_PHYPA	1.006e-10	78.95	NAD(P)H-quinone oxidoreductase subunit M, chloropl...	[more]
NDHM_ACAM1	1.776e-7	56.41	NAD(P)H-quinone oxidoreductase subunit M OS=Acaryo...	[more]
NDHM_MICAN	2.319e-7	63.16	NAD(P)H-quinone oxidoreductase subunit M OS=Microc...	[more]
NDHM_NOSP7	3.029e-7	51.11	NAD(P)H-quinone oxidoreductase subunit M OS=Nostoc...	[more]
NDHM_CYAA5	3.029e-7	57.89	NAD(P)H-quinone oxidoreductase subunit M OS=Cyanot...	[more]

BLAST of GH719223 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G37925.1	8.974e-16	71.74	\| Symbols: NDH-M \| subunit NDH-M of NAD(P)H:plasto...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018922	NAD(P)H-quinone oxidoreductase subunit M	PFAM	PF10664	NdhM	coord: 1..38 score: 6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719223 ID=GH719223; Name=GH719223; organism=Pisum sativum; type=EST; length=262bp
TTCGACTGATTGGTTCAGACATAGAGCACTATATAAGAAAGTTGTTATAT
GATGGAGTGATCAAGTATAATATGAATGCAAGGGTTTTGAATTTCAGCAT
GGGGAAGCCACGAATCATGTTCAATAACAATGATGTCCAACCTGACGATT
CCACTTGAGAAATCGTTTGTCTAATACACCAAGAAAAGTGAGCAAATCAT
TGATGTTTTTGTGTATGATGATGAATTGGTTACATATATATTTTTTAAAT
TATTACTTCCTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR018922	NdhM

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