EX569696

EST Overview
NameEX569696
Unique NameEX569696
TypeEST
OrganismPisum sativum (pea)
Sequence length527
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of EX569696 vs. TrEMBL
Match: B7FH50_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 9.951e-8
Identity = 35/80 (43.75%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  S + +    Y    N  NLAADE Q NGNS+ +  + IIIPGPA+ IPAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALQATNVEASFMTVLTEIY-NIVNKKNLAADESQGNGNSASLLGQKIIIPGPAQEIPAK 218
[Close]
BLAST of EX569696 vs. TrEMBL
Match: Q39844_SOYBN (Small GTP-binding protein (Fragment) OS=Glycine max GN=sra1 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.697e-7
Identity = 33/80 (41.25%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:  136 VPTEDAKEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 214
[Close]
BLAST of EX569696 vs. TrEMBL
Match: C6T447_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.895e-7
Identity = 32/74 (43.24%), Postives = 43/74 (58.11%), Query Frame = 1 
Query:   37 KRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:  149 KEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 221
[Close]
BLAST of EX569696 vs. TrEMBL
Match: C0STW5_TOBAC (NtRab11D OS=Nicotiana tabacum GN=NtRab11D PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.939e-7
Identity = 30/85 (35.29%), Postives = 46/85 (54.12%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            ++   +P    K    K+  F+ +TSA+   NV  + L +   +     N  NLAADE Q NGN + ++ K I++PGP +VIP K
Sbjct:  133 EDQRAVPTEDAKEFAQKEGLFFLETSAMEATNVEDAFLTVLT-EIFNIVNKKNLAADENQSNGNPASLTGKKILVPGPGQVIPGK 216
[Close]
BLAST of EX569696 vs. TrEMBL
Match: Q08147_PEA (GTP-binding protein OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 6.450e-7
Identity = 32/80 (40.00%), Postives = 44/80 (55.00%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  S + +    Y    N  NLAADE Q NGNS+ +  + I+IPGP + +PAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALQATNVEASFMTVLTEIY-NILNKKNLAADESQGNGNSASLLGQKIVIPGPGQEVPAK 218
[Close]
BLAST of EX569696 vs. SwissProt
Match: RB11A_LOTJA (Ras-related protein Rab11A OS=Lotus japonicus GN=RAB11A PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 2.902e-8
Identity = 34/80 (42.50%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  +   +    Y    N  +LAADE Q NGNS+ +S + IIIPGPA+ IPAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALEATNVESAFTTVLTEIY-NIVNKKSLAADESQGNGNSASLSGQKIIIPGPAQEIPAK 218
[Close]
BLAST of EX569696 vs. SwissProt
Match: RB11D_TOBAC (Ras-related protein Rab11D OS=Nicotiana tabacum GN=RAB11D PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.457e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            ++   +P    K    K+  F+ +TSA+   N+  + L +   +     N  NLAAD+ Q NGN + ++ K I++PGP +VIP K
Sbjct:  133 EDQRAVPTEDAKEFAQKEGLFFLETSAMEATNLEDAFLTVLT-EIFNIVNKKNLAADDNQSNGNPASLTGKKILVPGPGQVIPEK 216
[Close]
BLAST of EX569696 vs. TrEMBL
Match: B7FH50_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.180e-7
Identity = 35/80 (43.75%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  S + +    Y    N  NLAADE Q NGNS+ +  + IIIPGPA+ IPAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALQATNVEASFMTVLTEIY-NIVNKKNLAADESQGNGNSASLLGQKIIIPGPAQEIPAK 218
[Close]
BLAST of EX569696 vs. TrEMBL
Match: Q39844_SOYBN (Small GTP-binding protein (Fragment) OS=Glycine max GN=sra1 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.013e-7
Identity = 33/80 (41.25%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:  136 VPTEDAKEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 214
[Close]
BLAST of EX569696 vs. TrEMBL
Match: C6T447_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.434e-7
Identity = 32/74 (43.24%), Postives = 43/74 (58.11%), Query Frame = 1 
Query:   37 KRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:  149 KEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 221
[Close]
BLAST of EX569696 vs. TrEMBL
Match: C0STW5_TOBAC (NtRab11D OS=Nicotiana tabacum GN=NtRab11D PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.857e-7
Identity = 30/85 (35.29%), Postives = 46/85 (54.12%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            ++   +P    K    K+  F+ +TSA+   NV  + L +   +     N  NLAADE Q NGN + ++ K I++PGP +VIP K
Sbjct:  133 EDQRAVPTEDAKEFAQKEGLFFLETSAMEATNVEDAFLTVLT-EIFNIVNKKNLAADENQSNGNPASLTGKKILVPGPGQVIPGK 216
[Close]
BLAST of EX569696 vs. TrEMBL
Match: Q08147_PEA (GTP-binding protein OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 7.650e-7
Identity = 32/80 (40.00%), Postives = 44/80 (55.00%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  S + +    Y    N  NLAADE Q NGNS+ +  + I+IPGP + +PAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALQATNVEASFMTVLTEIY-NILNKKNLAADESQGNGNSASLLGQKIVIPGPGQEVPAK 218
[Close]
BLAST of EX569696 vs. Lotus protein
Match: LjSGA_053319.1 (+ phase: 2 /partial)

HSP 1 Score: 58.151 bits (139), Expect = 1.878e-9
Identity = 34/80 (42.50%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  +   +    Y    N  +LAADE Q NGNS+ +S + IIIPGPA+ IPAK
Sbjct:   62 VPTEDAKEFAEKEGLFFLETSALEATNVESAFTTVLTEIY-NIVNKKSLAADESQGNGNSASLSGQKIIIPGPAQEIPAK 140
[Close]
BLAST of EX569696 vs. Soybean peptides
Match: Glyma05g31020.2|PACid:16260243 ()

HSP 1 Score: 59.3066 bits (142), Expect = 2.137e-9
Identity = 33/80 (41.25%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:   77 VPTEDAKEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 155
[Close]
BLAST of EX569696 vs. Soybean peptides
Match: Glyma05g31020.1|PACid:16260242 ()

HSP 1 Score: 59.3066 bits (142), Expect = 2.137e-9
Identity = 33/80 (41.25%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  + + +    Y    N  NL ADE Q NGNS+ +S + II+PGPA+ IPAK
Sbjct:  143 VPTEDAKEFAEKEGLFFLETSALEATNVETAFITVLTEIY-NIVNKKNLTADENQGNGNSASLSGQKIIVPGPAQEIPAK 221
[Close]
BLAST of EX569696 vs. Soybean peptides
Match: Glyma18g01910.1|PACid:16307629 ()

HSP 1 Score: 53.5286 bits (127), Expect = 1.172e-7
Identity = 30/87 (34.48%), Postives = 46/87 (52.87%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAKIM 264
            +   ++P    K    K+  F+ +TSAL   NV  + + +   +     N  NLAA + Q N NS+ +S K II+PGPA+ IP + M
Sbjct:  135 ENQRQVPTEDAKEFAEKEGLFFLETSALEATNVETAFMTVLT-EIFNIINKKNLAASDNQGNDNSASLSGKKIIVPGPAQEIPKRSM 220
[Close]
BLAST of EX569696 vs. Soybean peptides
Match: Glyma11g38010.1|PACid:16285779 ()

HSP 1 Score: 51.9878 bits (123), Expect = 3.411e-7
Identity = 29/87 (33.33%), Postives = 46/87 (52.87%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAKIM 264
            +   ++P    K    K+  F+ +TSAL   NV  + + +   +     N  NLAA + Q NGN++ +S K II+PG A+ IP + M
Sbjct:  135 ENQRQVPTEDAKEFAEKEGLFFLETSALEATNVETAFMTVLT-EIFNIVNKKNLAAGDNQGNGNAASLSGKQIIVPGTAQEIPKRSM 220
[Close]
BLAST of EX569696 vs. SwissProt
Match: RB11A_LOTJA (Ras-related protein Rab11A OS=Lotus japonicus GN=RAB11A PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 2.955e-8
Identity = 34/80 (42.50%), Postives = 45/80 (56.25%), Query Frame = 1 
Query:   19 LPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            +P    K    K+  F+ +TSAL   NV  +   +    Y    N  +LAADE Q NGNS+ +S + IIIPGPA+ IPAK
Sbjct:  140 VPTEDAKEFAEKEGLFFLETSALEATNVESAFTTVLTEIY-NIVNKKSLAADESQGNGNSASLSGQKIIIPGPAQEIPAK 218
[Close]
BLAST of EX569696 vs. SwissProt
Match: RB11D_TOBAC (Ras-related protein Rab11D OS=Nicotiana tabacum GN=RAB11D PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.502e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAK 258
            ++   +P    K    K+  F+ +TSA+   N+  + L +   +     N  NLAAD+ Q NGN + ++ K I++PGP +VIP K
Sbjct:  133 EDQRAVPTEDAKEFAQKEGLFFLETSAMEATNLEDAFLTVLT-EIFNIVNKKNLAADDNQSNGNPASLTGKKILVPGPGQVIPEK 216
[Close]
BLAST of EX569696 vs. Medicago proteins
Match: IMGA|Medtr3g086980.1 (GTP-binding protein (AHRD V1 **** Q08147_PEA); contains Interpro domain(s) IPR015595 Rab11-related chr03_pseudomolecule_IMGAG_V3.5 28710998-28707447 F EGN_Mt100125 20100825)

HSP 1 Score: 62.3882 bits (150), Expect = 1.453e-10
Identity = 35/87 (40.23%), Postives = 48/87 (55.17%), Query Frame = 1 
Query:    4 DETNRLPLRLQKRLRIKKACFYYKTSALNTINVSYSLL*LFGHQYLTFTNNTNLAADEYQKNGNSSFVSDKNIIIPGPAKVIPAKIM 264
            +E   +P    K    K+  F+ +TSAL   NV  + + +   +     N  NLAADE Q NGNS+ +S K II+PGPA+ IP K M
Sbjct:  134 EEQRAVPTEDAKEFAEKEGLFFLETSALQATNVETAFMTVLT-EIFNIVNKKNLAADENQGNGNSASLSGKKIIVPGPAQEIPKKSM 219
The following BLAST results are available for this feature:
BLAST of EX569696 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
B7FH50_MEDTR9.951e-843.75Putative uncharacterized protein OS=Medicago trunc... [more]
Q39844_SOYBN1.697e-741.25Small GTP-binding protein (Fragment) OS=Glycine ma... [more]
C6T447_SOYBN2.895e-743.24Putative uncharacterized protein OS=Glycine max PE... [more]
C0STW5_TOBAC4.939e-735.29NtRab11D OS=Nicotiana tabacum GN=NtRab11D PE=2 SV=... [more]
Q08147_PEA6.450e-740.00GTP-binding protein OS=Pisum sativum PE=2 SV=1[more]
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BLAST of EX569696 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
RB11A_LOTJA2.902e-842.50Ras-related protein Rab11A OS=Lotus japonicus GN=R... [more]
RB11D_TOBAC2.457e-732.94Ras-related protein Rab11D OS=Nicotiana tabacum GN... [more]
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BLAST of EX569696 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
B7FH50_MEDTR1.180e-743.75Putative uncharacterized protein OS=Medicago trunc... [more]
Q39844_SOYBN2.013e-741.25Small GTP-binding protein (Fragment) OS=Glycine ma... [more]
C6T447_SOYBN3.434e-743.24Putative uncharacterized protein OS=Glycine max PE... [more]
C0STW5_TOBAC5.857e-735.29NtRab11D OS=Nicotiana tabacum GN=NtRab11D PE=2 SV=... [more]
Q08147_PEA7.650e-740.00GTP-binding protein OS=Pisum sativum PE=2 SV=1[more]
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BLAST of EX569696 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
LjSGA_053319.11.878e-942.50+ phase: 2 /partial[more]
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BLAST of EX569696 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 4
Match NameE-valueIdentityDescription
Glyma05g31020.2|PACid:162602432.137e-941.25[more]
Glyma05g31020.1|PACid:162602422.137e-941.25[more]
Glyma18g01910.1|PACid:163076291.172e-734.48[more]
Glyma11g38010.1|PACid:162857793.411e-733.33[more]
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BLAST of EX569696 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
RB11A_LOTJA2.955e-842.50Ras-related protein Rab11A OS=Lotus japonicus GN=R... [more]
RB11D_TOBAC2.502e-732.94Ras-related protein Rab11D OS=Nicotiana tabacum GN... [more]
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BLAST of EX569696 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 1
Match NameE-valueIdentityDescription
IMGA|Medtr3g086980.11.453e-1040.23GTP-binding protein (AHRD V1 **** Q08147_PEA); con... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX569696 ID=EX569696; Name=EX569696; organism=Pisum sativum; type=EST; length=527bp
GAGGACGAGACAAACCGCCTCCCACTCAGGTTGCAAAAAAGATTGCGGAT
CAAGAAGGCTTGTTTTTATTATAAGACCTCTGCACTGAACACAATTAACG
TAAGCTACAGCCTTTTATAACTGTTTGGCCATCAATATTTAACTTTTACC
AATAATACCAACCTGGCTGCTGATGAGTATCAGAAAAATGGCAACTCTTC
ATTTGTGTCTGACAAAAATATTATTATTCCTGGTCCAGCAAAAGTCATCC
CCGCCAAAATTATGGTGTTTTTACCGAAATTCTGACTTAGCTCTCGATTT
GTTTTCATATACTATATTATCATGGTTCTTTGTTCCTATCTTCAATTTTT
AATGTTTTGCCTCCGTTCCCTTGTATATTGACCTCTTTCAAAATTTATTT
TGCCTGACTTGTTGATTTAAGACCAGCCGATTAAATGCAAATTATGTACT
GCTTGTTATAATCCCAATTGATTTAATATAAGGCTTGGCTGGAATTGGAA
CATGTTGATTGAAAAATATTGTCATAC
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