EX570261

EST Overview


Name	EX570261
Unique Name	EX570261
Type	EST
Organism	Pisum sativum (pea)
Sequence length	588

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX570261 vs. TrEMBL
Match: Q2PEW1_TRIPR (Putative Argonaute protein (Fragment) OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 7.163e-62
Identity = 117/142 (82.39%), Postives = 117/142 (82.39%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSMTS                        VRPLPALKENVKRVMFYC
Sbjct:  159 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMTSGAVSRGGMGAAAGRSTRAPGANAAVRPLPALKENVKRVMFYC 300

[Close]

BLAST of EX570261 vs. TrEMBL
Match: A1E5M3_PEA (Argonaute 1 OS=Pisum sativum GN=AGO1 PE=2 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 7.163e-62
Identity = 117/142 (82.39%), Postives = 117/142 (82.39%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSMTS                        VRPLPALKENVKRVMFYC
Sbjct:  959 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMTSGAVPRGGMAAAAGRSSRAPGANAAVRPLPALKENVKRVMFYC 1100

[Close]

BLAST of EX570261 vs. TrEMBL
Match: D6RUV9_TOBAC (ARGONAUTE 1 OS=Nicotiana tabacum GN=AGO1 PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 2.084e-61
Identity = 116/142 (81.69%), Postives = 117/142 (82.39%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGS+TS                        VRPLPALKENVKRVMFYC
Sbjct:  920 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSVTSAAASNRGGVGAMGRSTRAPGAGAAVRPLPALKENVKRVMFYC 1061

[Close]

BLAST of EX570261 vs. TrEMBL
Match: Q2LFC4_NICBE (AGO1-1 (Fragment) OS=Nicotiana benthamiana PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 4.643e-61
Identity = 115/142 (80.99%), Postives = 117/142 (82.39%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SD+GS+TS                        VRPLPALKENVKRVMFYC
Sbjct:  911 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDNGSVTSAAASNRGGLGAMGRSTRAPGAGAAVRPLPALKENVKRVMFYC 1052

[Close]

BLAST of EX570261 vs. TrEMBL
Match: B9N365_POPTR (Argonaute protein group OS=Populus trichocarpa GN=AGO911 PE=4 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 2.304e-60
Identity = 114/142 (80.28%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGS+TS                        VRPLPALKENVKRVMFYC
Sbjct:  541 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSLTSGMASGRGGGGAGGRATRGPAANAAVRPLPALKENVKRVMFYC 682

[Close]

BLAST of EX570261 vs. TrEMBL
Match: D5ACK3_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 6.705e-60
Identity = 113/142 (79.58%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGS++S                        VRPLPALKENVKRVMFYC
Sbjct:   88 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSLSSGVPGSRMTAASSGSRSTRVPAGTTVRPLPALKENVKRVMFYC 229

[Close]

BLAST of EX570261 vs. TrEMBL
Match: Q3ECU7_ARATH (Putative uncharacterized protein At1g48410.2 OS=Arabidopsis thaliana GN=At1g48410 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.494e-59
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  911 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC 1050

[Close]

BLAST of EX570261 vs. TrEMBL
Match: D7KD09_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473977 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.494e-59
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  913 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRNTRGPNINAAVRPLPALKENVKRVMFYC 1052

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BLAST of EX570261 vs. TrEMBL
Match: D7TXS1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_53.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029383001 PE=4 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.951e-59
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSMTS                        VRPLPALKENVKRVMFYC
Sbjct:  916 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMTS-------------------GAAAAVRPLPALKENVKRVMFYC 1038

[Close]

BLAST of EX570261 vs. TrEMBL
Match: B9SJV6_RICCO (Eukaryotic translation initiation factor 2c, putative OS=Ricinus communis GN=RCOM_0605410 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.327e-59
Identity = 115/144 (79.86%), Postives = 115/144 (79.86%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTS--XXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSMTS                          VRPLPALKENVKRVMFYC
Sbjct:  920 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMTSGPVGGRGGMGGGAGARSTRGPAASAAVRPLPALKENVKRVMFYC 1063

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO1_ARATH (Protein argonaute OS=Arabidopsis thaliana GN=AGO1 PE=1 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.085e-60
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  909 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC 1048

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO1B_ORYSJ (Protein argonaute 1B OS=Oryza sativa subsp. japonica GN=AGO1B PE=2 SV=3)

HSP 1 Score: 228.794 bits (582), Expect = 1.567e-59
Identity = 113/142 (79.58%), Postives = 114/142 (80.28%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQ+LTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  979 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GAATSRGLPPGVRSARVAGNVAVRPLPALKENVKRVMFYC 1118

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO1A_ORYSJ (Protein argonaute 1A OS=Oryza sativa subsp. japonica GN=AGO1A PE=2 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.327e-58
Identity = 111/142 (78.17%), Postives = 113/142 (79.58%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDEN FTAD LQ+LTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEP+ SDSGSM S                        VRPLP LKENVKRVMFYC
Sbjct:  942 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPDTSDSGSMAS-GAHTRGGGPLPGARSTKPAGNVAVRPLPDLKENVKRVMFYC 1082

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO1D_ORYSJ (Protein argonaute 1D OS=Oryza sativa subsp. japonica GN=AGO1D PE=2 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 3.268e-57
Identity = 108/142 (76.06%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDF+LCSHAGI+GTSRPAHYHVLWDENNFTADALQ+LTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYME ++SDSGSM S                        VRPLPALK++VK VMFYC
Sbjct:  902 ILPGTVVDSKICHPTEFDFFLCSHAGIKGTSRPAHYHVLWDENNFTADALQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMESDSSDSGSMAS-----GRGGGSSTSRSTRAAGGGAVRPLPALKDSVKNVMFYC 1038

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO1C_ORYSJ (Protein argonaute 1C OS=Oryza sativa subsp. japonica GN=AGO1C PE=2 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 3.268e-57
Identity = 108/142 (76.06%), Postives = 114/142 (80.28%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGI+GTSRPAHYHVLWDENNFTADALQ LTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEP+ SDS S+ S                        V+PLPALK++VKRVMFYC
Sbjct:  871 ILPGTVVDSKICHPTEFDFYLCSHAGIKGTSRPAHYHVLWDENNFTADALQILTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPDTSDSSSVVS-GPGVRGPLSGSSTSRTRAPGGAAVKPLPALKDSVKRVMFYC 1011

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BLAST of EX570261 vs. SwissProt
Match: PINH_ARATH (Protein PINHEAD OS=Arabidopsis thaliana GN=At5g43810 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.373e-55
Identity = 107/143 (74.83%), Postives = 112/143 (78.32%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE-NSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVD+KICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD +QSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFY+EPE   D+GS                           V+PLPALKENVKRVMFYC
Sbjct:  858 ILPGTVVDTKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGIQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYLEPEIMQDNGS------------PGKKNTKTTTVGDVGVKPLPALKENVKRVMFYC 988

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BLAST of EX570261 vs. SwissProt
Match: PNH1_ORYSJ (Protein argonaute PNH1 OS=Oryza sativa subsp. japonica GN=PHN1 PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.162e-54
Identity = 102/142 (71.83%), Postives = 112/142 (78.87%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHP+EFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD +Q+LTNNLCYTYARCTRSVS+VPPAYYAHLAAFRARFYMEPE S++ + +                         V+PLPA+KE VKRVMFYC
Sbjct:  853 ILPGTVVDSKICHPSEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADEMQTLTNNLCYTYARCTRSVSVVPPAYYAHLAAFRARFYMEPEMSENQTTSK---------------SSTGTNGTSVKPLPAVKEKVKRVMFYC 979

[Close]

BLAST of EX570261 vs. SwissProt
Match: MEL1_ORYSJ (Protein argonaute MEL1 OS=Oryza sativa subsp. japonica GN=MEL1 PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 1.571e-51
Identity = 99/142 (69.72%), Postives = 108/142 (76.06%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVD +ICHPTEFDFYLCSHAGIQGTSRP HYHVL+DEN+FTADALQSLTNNLCYTYARCTR+VS+VPPAYYAHLAAFRAR+Y+E E+SD GS                           VR LP +KENVK VMFYC
Sbjct:  928 ILPGTVVDRQICHPTEFDFYLCSHAGIQGTSRPTHYHVLYDENHFTADALQSLTNNLCYTYARCTRAVSVVPPAYYAHLAAFRARYYVEGESSDGGSTPG-----------SSGQAVAREGPVEVRQLPKIKENVKDVMFYC 1058

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BLAST of EX570261 vs. SwissProt
Match: AGOL_ARATH (Argonaute-like protein At2g27880 OS=Arabidopsis thaliana GN=At2g27880 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 9.530e-49
Identity = 97/142 (68.31%), Postives = 103/142 (72.54%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            I PGTVVD+KICHP EFDFYL SHAGIQGTSRPAHYHVL DEN FTAD LQ LTNNLCYTYARCT+SVSIVPPAYYAHLAAFRAR+YME E SD GS  S                        +  LPA+K+NVK VMFYC
Sbjct:  870 IQPGTVVDTKICHPNEFDFYLNSHAGIQGTSRPAHYHVLLDENGFTADQLQMLTNNLCYTYARCTKSVSIVPPAYYAHLAAFRARYYMESEMSDGGSSRS--------------RSSTTGVGQVISQLPAIKDNVKEVMFYC 997

[Close]

BLAST of EX570261 vs. SwissProt
Match: AGO12_ORYSJ (Protein argonaute 12 OS=Oryza sativa subsp. japonica GN=AGO12 PE=2 SV=2)

HSP 1 Score: 188.348 bits (477), Expect = 2.347e-47
Identity = 92/142 (64.79%), Postives = 103/142 (72.54%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVD+ ICHP+EFDFYLCSH+GI+GTSRP HYHVL DEN F AD LQ+LT NL YTYARCTR+VSIVPPAYYAHL AFRAR+YME E+SD GS +S                         +PLP +KENVKR MFYC
Sbjct:  924 ILPGTVVDTMICHPSEFDFYLCSHSGIKGTSRPTHYHVLLDENGFKADTLQTLTYNLSYTYARCTRAVSIVPPAYYAHLGAFRARYYMEDEHSDQGSSSS----------------VTTRTDRSTKPLPEIKENVKRFMFYC 1049

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT1G48410.3 (| Symbols: AGO1 | Stabilizer of iron transporter SufD / Polynucleotidyl transferase | chr1:17886285-17891892 REVERSE LENGTH=1050)

HSP 1 Score: 232.646 bits (592), Expect = 1.144e-61
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  911 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC 1050

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT1G48410.2 (| Symbols: AGO1 | Stabilizer of iron transporter SufD / Polynucleotidyl transferase | chr1:17886285-17891892 REVERSE LENGTH=1050)

HSP 1 Score: 232.646 bits (592), Expect = 1.144e-61
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  911 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC 1050

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT1G48410.1 (| Symbols: AGO1 | Stabilizer of iron transporter SufD / Polynucleotidyl transferase | chr1:17886285-17891892 REVERSE LENGTH=1048)

HSP 1 Score: 232.646 bits (592), Expect = 1.144e-61
Identity = 115/142 (80.99%), Postives = 115/142 (80.99%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD LQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE SDSGSM S                        VRPLPALKENVKRVMFYC
Sbjct:  909 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMAS--GSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC 1048

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT5G43810.2 (| Symbols: ZLL | Stabilizer of iron transporter SufD / Polynucleotidyl transferase | chr5:17611939-17616562 FORWARD LENGTH=988)

HSP 1 Score: 215.698 bits (548), Expect = 1.447e-56
Identity = 107/143 (74.83%), Postives = 112/143 (78.32%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE-NSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVD+KICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD +QSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFY+EPE   D+GS                           V+PLPALKENVKRVMFYC
Sbjct:  858 ILPGTVVDTKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGIQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYLEPEIMQDNGS------------PGKKNTKTTTVGDVGVKPLPALKENVKRVMFYC 988

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT5G43810.1 (| Symbols: ZLL, PNH, AGO10 | Stabilizer of iron transporter SufD / Polynucleotidyl transferase | chr5:17611939-17616562 FORWARD LENGTH=988)

HSP 1 Score: 215.698 bits (548), Expect = 1.447e-56
Identity = 107/143 (74.83%), Postives = 112/143 (78.32%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPE-NSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            ILPGTVVD+KICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTAD +QSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFY+EPE   D+GS                           V+PLPALKENVKRVMFYC
Sbjct:  858 ILPGTVVDTKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGIQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYLEPEIMQDNGS------------PGKKNTKTTTVGDVGVKPLPALKENVKRVMFYC 988

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT2G27880.1 (| Symbols: AGO5 | Argonaute family protein | chr2:11871488-11876712 FORWARD LENGTH=997)

HSP 1 Score: 192.971 bits (489), Expect = 1.004e-49
Identity = 97/142 (68.31%), Postives = 103/142 (72.54%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            I PGTVVD+KICHP EFDFYL SHAGIQGTSRPAHYHVL DEN FTAD LQ LTNNLCYTYARCT+SVSIVPPAYYAHLAAFRAR+YME E SD GS  S                        +  LPA+K+NVK VMFYC
Sbjct:  870 IQPGTVVDTKICHPNEFDFYLNSHAGIQGTSRPAHYHVLLDENGFTADQLQMLTNNLCYTYARCTKSVSIVPPAYYAHLAAFRARYYMESEMSDGGSSRS--------------RSSTTGVGQVISQLPAIKDNVKEVMFYC 997

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT1G69440.1 (| Symbols: AGO7, ZIP | Argonaute family protein | chr1:26101565-26105016 REVERSE LENGTH=990)

HSP 1 Score: 162.925 bits (411), Expect = 1.113e-40
Identity = 79/143 (55.24%), Postives = 92/143 (64.34%), Query Frame = 2

Query:    2 ILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYME-PENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            I PGTVVD+ I HP EFDFYLCSH G++GTSRP HYH+LWDEN FT+D LQ L  NLCYT+ RCT+ +SIVPPAYYAHLAA+R R Y+E    S+ GSM                            PLP L +NVK +MFYC
Sbjct:  862 IPPGTVVDTVITHPKEFDFYLCSHLGVKGTSRPTHYHILWDENEFTSDELQRLVYNLCYTFVRCTKPISIVPPAYYAHLAAYRGRLYIERSSESNGGSMNPSSVSRVGPPKTI--------------PLPKLSDNVKNLMFYC 990

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT2G27040.2 (| Symbols: AGO4 | Argonaute family protein | chr2:11536795-11541503 REVERSE LENGTH=924)

HSP 1 Score: 120.939 bits (302), Expect = 4.851e-28
Identity = 61/140 (43.57%), Postives = 81/140 (57.86%), Query Frame = 2

Query:    8 PGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            PGT++D+KICHP   DFYLC+HAG+ GT+RP HYHVL+DE  F+AD LQ L ++L Y Y R T ++S+V P  YAHLAA +   +M+ E+    S +                         V  LP LK+NV   MF+C
Sbjct:  799 PGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSS--------------HGGITAPGPISVAQLPRLKDNVANSMFFC 924

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT2G27040.1 (| Symbols: AGO4, OCP11 | Argonaute family protein | chr2:11536795-11541503 REVERSE LENGTH=924)

HSP 1 Score: 120.939 bits (302), Expect = 4.851e-28
Identity = 61/140 (43.57%), Postives = 81/140 (57.86%), Query Frame = 2

Query:    8 PGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSDSGSMTSXXXXXXXXXXXXXXXXXXXXXXXXVRPLPALKENVKRVMFYC 427
            PGT++D+KICHP   DFYLC+HAG+ GT+RP HYHVL+DE  F+AD LQ L ++L Y Y R T ++S+V P  YAHLAA +   +M+ E+    S +                         V  LP LK+NV   MF+C
Sbjct:  799 PGTIIDNKICHPKNNDFYLCAHAGMIGTTRPTHYHVLYDEIGFSADELQELVHSLSYVYQRSTSAISVVAPICYAHLAAAQLGTFMKFEDQSETSSS--------------HGGITAPGPISVAQLPRLKDNVANSMFFC 924

[Close]

BLAST of EX570261 vs. TAIR peptide
Match: AT1G31280.1 (| Symbols: AGO2 | Argonaute family protein | chr1:11181777-11185112 FORWARD LENGTH=1014)

HSP 1 Score: 120.939 bits (302), Expect = 4.851e-28
Identity = 51/91 (56.04%), Postives = 66/91 (72.53%), Query Frame = 2

Query:   11 GTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADALQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPENSD 283
            GTVVD+K+ HP E+DFYLCSH G  GTS+P HY+ LWDE  FT+D +Q L   +C+T+ RCT+ VS+VPP YYA + AFR R Y E  + +
Sbjct:  880 GTVVDTKVIHPYEYDFYLCSHHGGIGTSKPTHYYTLWDELGFTSDQVQKLIFEMCFTFTRCTKPVSLVPPVYYADMVAFRGRMYHEASSRE 970

The following BLAST results are available for this feature:

BLAST of EX570261 vs. TrEMBL
BLAST of EX570261 vs. SwissProt
BLAST of EX570261 vs. TAIR peptide

BLAST of EX570261 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2PEW1_TRIPR	7.163e-62	82.39	Putative Argonaute protein (Fragment) OS=Trifolium...	[more]
A1E5M3_PEA	7.163e-62	82.39	Argonaute 1 OS=Pisum sativum GN=AGO1 PE=2 SV=1	[more]
D6RUV9_TOBAC	2.084e-61	81.69	ARGONAUTE 1 OS=Nicotiana tabacum GN=AGO1 PE=2 SV=1	[more]
Q2LFC4_NICBE	4.643e-61	80.99	AGO1-1 (Fragment) OS=Nicotiana benthamiana PE=2 SV...	[more]
B9N365_POPTR	2.304e-60	80.28	Argonaute protein group OS=Populus trichocarpa GN=...	[more]
D5ACK3_PICSI	6.705e-60	79.58	Putative uncharacterized protein OS=Picea sitchens...	[more]
Q3ECU7_ARATH	1.494e-59	80.99	Putative uncharacterized protein At1g48410.2 OS=Ar...	[more]
D7KD09_ARALY	1.494e-59	80.99	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TXS1_VITVI	1.951e-59	80.99	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SJV6_RICCO	3.327e-59	79.86	Eukaryotic translation initiation factor 2c, putat...	[more]

BLAST of EX570261 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AGO1_ARATH	1.085e-60	80.99	Protein argonaute OS=Arabidopsis thaliana GN=AGO1 ...	[more]
AGO1B_ORYSJ	1.567e-59	79.58	Protein argonaute 1B OS=Oryza sativa subsp. japoni...	[more]
AGO1A_ORYSJ	1.327e-58	78.17	Protein argonaute 1A OS=Oryza sativa subsp. japoni...	[more]
AGO1D_ORYSJ	3.268e-57	76.06	Protein argonaute 1D OS=Oryza sativa subsp. japoni...	[more]
AGO1C_ORYSJ	3.268e-57	76.06	Protein argonaute 1C OS=Oryza sativa subsp. japoni...	[more]
PINH_ARATH	1.373e-55	74.83	Protein PINHEAD OS=Arabidopsis thaliana GN=At5g438...	[more]
PNH1_ORYSJ	1.162e-54	71.83	Protein argonaute PNH1 OS=Oryza sativa subsp. japo...	[more]
MEL1_ORYSJ	1.571e-51	69.72	Protein argonaute MEL1 OS=Oryza sativa subsp. japo...	[more]
AGOL_ARATH	9.530e-49	68.31	Argonaute-like protein At2g27880 OS=Arabidopsis th...	[more]
AGO12_ORYSJ	2.347e-47	64.79	Protein argonaute 12 OS=Oryza sativa subsp. japoni...	[more]

BLAST of EX570261 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G48410.3	1.144e-61	80.99	\| Symbols: AGO1 \| Stabilizer of iron transporter S...	[more]
AT1G48410.2	1.144e-61	80.99	\| Symbols: AGO1 \| Stabilizer of iron transporter S...	[more]
AT1G48410.1	1.144e-61	80.99	\| Symbols: AGO1 \| Stabilizer of iron transporter S...	[more]
AT5G43810.2	1.447e-56	74.83	\| Symbols: ZLL \| Stabilizer of iron transporter Su...	[more]
AT5G43810.1	1.447e-56	74.83	\| Symbols: ZLL, PNH, AGO10 \| Stabilizer of iron tr...	[more]
AT2G27880.1	1.004e-49	68.31	\| Symbols: AGO5 \| Argonaute family protein \| chr2:...	[more]
AT1G69440.1	1.113e-40	55.24	\| Symbols: AGO7, ZIP \| Argonaute family protein \| ...	[more]
AT2G27040.2	4.851e-28	43.57	\| Symbols: AGO4 \| Argonaute family protein \| chr2:...	[more]
AT2G27040.1	4.851e-28	43.57	\| Symbols: AGO4, OCP11 \| Argonaute family protein ...	[more]
AT1G31280.1	4.851e-28	56.04	\| Symbols: AGO2 \| Argonaute family protein \| chr1:...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003165	Stem cell self-renewal protein Piwi	PFAM	PF02171	Piwi	coord: 1..88 score: 1.9
IPR003165	Stem cell self-renewal protein Piwi	PROFILE	PS50822	PIWI	coord: 1..89 score: 16
IPR012337	Ribonuclease H-like	SUPERFAMILY	53098	Ribonuclease H-like	coord: 1..92 score: 2.6
None	No IPR available	PANTHER	PTHR22891	EUKARYOTIC TRANSLATION INITIATION FACTOR 2C	coord: 1..142 score: 3.6
None	No IPR available	SEG	seg	seg	coord: 101..124 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570261 ID=EX570261; Name=EX570261; organism=Pisum sativum; type=EST; length=588bp
CATATTACCTGGAACTGTTGTGGATTCTAAGATCTGCCATCCGACTGAGT
TTGACTTTTATCTATGCAGTCATGCCGGAATACAGGGTACAAGCCGTCCA
GCTCATTATCATGTTTTGTGGGATGAAAATAATTTTACTGCCGATGCTTT
GCAAAGTCTAACTAACAACTTGTGCTACACATATGCCAGATGTACCCGTT
CTGTTTCAATTGTGCCCCCTGCTTATTATGCTCATTTGGCTGCATTCCGA
GCAAGGTTCTATATGGAACCCGAGAATTCTGATAGTGGATCTATGACAAG
CGGTGCAGTTCCACGCGGTGGAATGGCAGCTGCTGCAGGGCGTAGTTCAC
GTGCCCCGGGTGCAAATGCTGCTGTTAGACCCTTGCCTGCGTTGAAGGAG
AATGTCAAGAGGGTCATGTTTTATTGCTGAGGTTTATTTAAAGTTTTTTG
AAGGACTGCTGAGATCATAATTGAAGTTTGATGAAGGACTGCTGGGATCA
TATTTGGAGTTTGTTGAAGGAAGTATCAAATAAACTGGTCTAGTTATGTT
GAAGTAATATTGAGATGATATTGACAAGTGTTTGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR003165	Piwi
IPR012337	RNaseH-like_sf

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