FG530421

EST Overview


Name	FG530421
Unique Name	FG530421
Type	EST
Organism	Pisum sativum (pea)
Sequence length	564

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Swissprot	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG530421 vs. TrEMBL
Match: B9SDG4_RICCO (Delta DNA polymerase, putative OS=Ricinus communis GN=RCOM_1517510 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.084e-22
Identity = 44/69 (63.77%), Postives = 53/69 (76.81%), Query Frame = 2

Query:  179 ALWI*DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            AL + D+  ++E VLRQFDMN  YGPC+GMTR+ RWERA  LGLNPP++IE LLK GKV  E LWD R+
Sbjct:   58 ALDLQDDYDKNEEVLRQFDMNMAYGPCLGMTRVGRWERAQRLGLNPPKQIEDLLKGGKVNSECLWDGRV 126

HSP 2 Score: 33.4982 bits (75), Expect = 1.084e-22
Identity = 16/30 (53.33%), Postives = 17/30 (56.67%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            K  P HAA   SN+ QP AL S  G  DLQ
Sbjct:   33 KSPPKHAAAIGSNITQPTALISHGGALDLQ 62

[Close]

BLAST of FG530421 vs. TrEMBL
Match: B9IPX7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_665537 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.243e-20
Identity = 43/64 (67.19%), Postives = 49/64 (76.56%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D+  + E VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK  KV+ E LWD  I
Sbjct:   58 DDYDKHEDVLRQFDMNPAYGPCLGMSRLARWERAQRLGLNPPKEIEGLLKGEKVRSECLWDGCI 121

HSP 2 Score: 31.187 bits (69), Expect = 1.243e-20
Identity = 16/31 (51.61%), Postives = 21/31 (67.74%), Query Frame = 3

Query:    3 TKKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            +K S IHAAT  S++ QP AL S +  PDL+
Sbjct:   28 SKSSLIHAATLGSDITQPTALVSHD-SPDLK 57

[Close]

BLAST of FG530421 vs. TrEMBL
Match: Q1H568_ARATH (At1g09815 OS=Arabidopsis thaliana GN=At1g09815 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.105e-20
Identity = 42/64 (65.62%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWERAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 2.105e-20
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

BLAST of FG530421 vs. TrEMBL
Match: Q8L9H3_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.638e-20
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RW+RAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWQRAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 4.638e-20
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

BLAST of FG530421 vs. TrEMBL
Match: D7KJU8_ARALY (DNA polymerase delta subunit 4 family OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471085 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 6.726e-19
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERA+ LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   59 EDYDKEEEMLRQFDMNIAYGPCLGMTRLDRWERALRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: D7T7F4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024265001 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 7.437e-18
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWD 376
            DE   +E+VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK GK + + LWD
Sbjct:   54 DEYDGNETVLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPKEIETLLKQGKARADCLWD 114

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A5AG58_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024389 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 7.437e-18
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWD 376
            DE   +E+VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK GK + + LWD
Sbjct:   59 DEYDGNETVLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPKEIETLLKQGKARADCLWD 119

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9PI30_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_571392 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.403e-16
Identity = 41/64 (64.06%), Postives = 46/64 (71.88%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D   E E +LRQFDMN  YGPC+GM+RLARWERA  LGLNPP E E LLK+  VQ E  WD R+
Sbjct:   59 DYYDEHEELLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPNEFEGLLKAEMVQSECWWDGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9PA85_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.403e-16
Identity = 41/64 (64.06%), Postives = 46/64 (71.88%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D   E E +LRQFDMN  YGPC+GM+RLARWERA  LGLNPP E E LLK+  VQ E  WD R+
Sbjct:   59 DYYDEHEGLLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPNEFEGLLKAEMVQSECWWDGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9NT40_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.575e-12
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D+    E VLRQFDMN  YGPCIG+ RL RWERA  LGL+PPQ+++  L+      + LW+ R+
Sbjct:   72 DDIDNMEEVLRQFDMNMKYGPCIGVPRLERWERASKLGLDPPQQVKVFLERAGGISDCLWEGRV 135

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_BOVIN (DNA polymerase delta subunit 4 OS=Bos taurus GN=POLD4 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.582e-8
Identity = 25/47 (53.19%), Postives = 33/47 (70.21%), Query Frame = 2

Query:  206 ESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKS 346
            E   +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++L+S
Sbjct:   44 EELELLRQFDLAWQYGPCTGITRLQRWHRAEQMGLKPPPEVHQVLQS 90

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_MOUSE (DNA polymerase delta subunit 4 OS=Mus musculus GN=Pold4 PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.752e-8
Identity = 28/55 (50.91%), Postives = 36/55 (65.45%), Query Frame = 2

Query:  218 VLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQ---QESLW 373
            +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++LK+       Q SLW
Sbjct:   48 LLRQFDLAWQYGPCTGITRLQRWSRAEQMGLKPPLEVYQVLKAHPEDPHFQCSLW 102

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_HUMAN (DNA polymerase delta subunit 4 OS=Homo sapiens GN=POLD4 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.812e-8
Identity = 26/51 (50.98%), Postives = 34/51 (66.67%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKS 346
            DE      +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++LK+
Sbjct:   40 DEEEAELELLRQFDLAWQYGPCTGITRLQRWCRAKQMGLEPPPEVWQVLKT 90

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_SCHPO (DNA polymerase delta subunit 4 OS=Schizosaccharomyces pombe GN=cdm1 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 4.870e-7
Identity = 22/58 (37.93%), Postives = 36/58 (62.07%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQES 367
            +  ++ E +L  FD    YGP +GMTR+ RW+RA N  LNPP+ + ++L   +  +E+
Sbjct:   88 ENITKVEFILHHFDTTARYGPYLGMTRMQRWKRAKNFNLNPPETVGKILMLEEADEEN 145

[Close]

BLAST of FG530421 vs. TAIR peptide
Match: AT1G09815.1 (| Symbols: POLD4 | polymerase delta 4 | chr1:3189460-3190050 FORWARD LENGTH=124)

HSP 1 Score: 98.5969 bits (244), Expect = 1.588e-22
Identity = 42/64 (65.62%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWERAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 1.588e-22
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWERAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 1.588e-22
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

BLAST of FG530421 vs. TrEMBL
Match: B9SDG4_RICCO (Delta DNA polymerase, putative OS=Ricinus communis GN=RCOM_1517510 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.325e-22
Identity = 44/69 (63.77%), Postives = 53/69 (76.81%), Query Frame = 2

Query:  179 ALWI*DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            AL + D+  ++E VLRQFDMN  YGPC+GMTR+ RWERA  LGLNPP++IE LLK GKV  E LWD R+
Sbjct:   58 ALDLQDDYDKNEEVLRQFDMNMAYGPCLGMTRVGRWERAQRLGLNPPKQIEDLLKGGKVNSECLWDGRV 126

HSP 2 Score: 33.4982 bits (75), Expect = 1.325e-22
Identity = 16/30 (53.33%), Postives = 17/30 (56.67%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            K  P HAA   SN+ QP AL S  G  DLQ
Sbjct:   33 KSPPKHAAAIGSNITQPTALISHGGALDLQ 62

[Close]

BLAST of FG530421 vs. TrEMBL
Match: B9IPX7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_665537 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.520e-20
Identity = 43/64 (67.19%), Postives = 49/64 (76.56%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D+  + E VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK  KV+ E LWD  I
Sbjct:   58 DDYDKHEDVLRQFDMNPAYGPCLGMSRLARWERAQRLGLNPPKEIEGLLKGEKVRSECLWDGCI 121

HSP 2 Score: 31.187 bits (69), Expect = 1.520e-20
Identity = 16/31 (51.61%), Postives = 21/31 (67.74%), Query Frame = 3

Query:    3 TKKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            +K S IHAAT  S++ QP AL S +  PDL+
Sbjct:   28 SKSSLIHAATLGSDITQPTALVSHD-SPDLK 57

[Close]

BLAST of FG530421 vs. TrEMBL
Match: Q1H568_ARATH (At1g09815 OS=Arabidopsis thaliana GN=At1g09815 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.574e-20
Identity = 42/64 (65.62%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWERAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 2.574e-20
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

BLAST of FG530421 vs. TrEMBL
Match: Q8L9H3_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 5.671e-20
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RW+RAV LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   61 EDYDKEEEMLRQFDMNITYGPCLGMTRLDRWQRAVRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 124

HSP 2 Score: 24.6386 bits (52), Expect = 5.671e-20
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3

Query:    6 KKSPIHAATFSSNLAQPPALTSRNGKPDL 92
            K +P HAA   S++ QP AL S +G  DL
Sbjct:   32 KVAPKHAAAQGSDVTQPAALIS-HGSVDL 59

[Close]

BLAST of FG530421 vs. TrEMBL
Match: D7KJU8_ARALY (DNA polymerase delta subunit 4 family OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_471085 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.223e-19
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            ++  + E +LRQFDMN  YGPC+GMTRL RWERA+ LG+NPP EIE+LLK+GKVQQ+ LW  R+
Sbjct:   59 EDYDKEEEMLRQFDMNIAYGPCLGMTRLDRWERALRLGMNPPNEIEKLLKTGKVQQDCLWQGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: D7T7F4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024265001 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 9.091e-18
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWD 376
            DE   +E+VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK GK + + LWD
Sbjct:   54 DEYDGNETVLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPKEIETLLKQGKARADCLWD 114

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A5AG58_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024389 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 9.091e-18
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWD 376
            DE   +E+VLRQFDMN  YGPC+GM+RLARWERA  LGLNPP+EIE LLK GK + + LWD
Sbjct:   59 DEYDGNETVLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPKEIETLLKQGKARADCLWD 119

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9PI30_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_571392 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.715e-16
Identity = 41/64 (64.06%), Postives = 46/64 (71.88%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D   E E +LRQFDMN  YGPC+GM+RLARWERA  LGLNPP E E LLK+  VQ E  WD R+
Sbjct:   59 DYYDEHEELLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPNEFEGLLKAEMVQSECWWDGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9PA85_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.715e-16
Identity = 41/64 (64.06%), Postives = 46/64 (71.88%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D   E E +LRQFDMN  YGPC+GM+RLARWERA  LGLNPP E E LLK+  VQ E  WD R+
Sbjct:   59 DYYDEHEGLLRQFDMNMAYGPCLGMSRLARWERAQRLGLNPPNEFEGLLKAEMVQSECWWDGRV 122

[Close]

BLAST of FG530421 vs. TrEMBL
Match: A9NT40_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 4.370e-12
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            D+    E VLRQFDMN  YGPCIG+ RL RWERA  LGL+PPQ+++  L+      + LW+ R+
Sbjct:   72 DDIDNMEEVLRQFDMNMKYGPCIGVPRLERWERASKLGLDPPQQVKVFLERAGGISDCLWEGRV 135

[Close]

BLAST of FG530421 vs. Lotus protein
Match: chr4.CM0337.20.r2.d (- phase: 0 )

HSP 1 Score: 103.605 bits (257), Expect = 4.377e-23
Identity = 49/64 (76.56%), Postives = 54/64 (84.38%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            DE + +E+VLRQFD+N  YGPCIGM RLARWERA  LGLNPPQEIERLLKSGKVQ ESLW+  I
Sbjct:   45 DESNGNEAVLRQFDLNMAYGPCIGMPRLARWERAQKLGLNPPQEIERLLKSGKVQAESLWNGCI 108

[Close]

BLAST of FG530421 vs. Soybean peptides
Match: Glyma05g25400.1|PACid:16259574 ()

HSP 1 Score: 109.383 bits (272), Expect = 1.008e-27
Identity = 51/64 (79.69%), Postives = 54/64 (84.38%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            DE  ESE  LRQFD+N  YGPC+GMTRLARWERA  LGLNPPQEI+RLLKSGKVQ ESLWD RI
Sbjct:   49 DEYKESEVALRQFDLNMAYGPCLGMTRLARWERAQRLGLNPPQEIDRLLKSGKVQTESLWDGRI 112

HSP 2 Score: 31.9574 bits (71), Expect = 1.008e-27
Identity = 18/31 (58.06%), Postives = 20/31 (64.52%), Query Frame = 3

Query:    3 TKKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            +KKSPI AA      AQ PAL S  GKPDL+
Sbjct:   24 SKKSPIPAA------AQSPALISHGGKPDLE 48

[Close]

BLAST of FG530421 vs. Soybean peptides
Match: Glyma08g08420.1|PACid:16270411 ()

HSP 1 Score: 106.686 bits (265), Expect = 1.256e-24
Identity = 51/64 (79.69%), Postives = 53/64 (82.81%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            DE  ESE VLRQFD+N  YGPC+GMTRLARWERA  L LNPPQEIERLLKSGKV  ESLWD RI
Sbjct:   41 DEHKESEVVLRQFDLNMAYGPCLGMTRLARWERAQRLDLNPPQEIERLLKSGKVPTESLWDGRI 104

HSP 2 Score: 24.2534 bits (51), Expect = 1.256e-24
Identity = 14/31 (45.16%), Postives = 17/31 (54.84%), Query Frame = 3

Query:    3 TKKSPIHAATFSSNLAQPPALTSRNGKPDLQ 95
            TKKSPI +          PA+ S  GKPDL+
Sbjct:   20 TKKSPILS----------PAIISLGGKPDLE 40

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_BOVIN (DNA polymerase delta subunit 4 OS=Bos taurus GN=POLD4 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.629e-8
Identity = 25/47 (53.19%), Postives = 33/47 (70.21%), Query Frame = 2

Query:  206 ESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKS 346
            E   +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++L+S
Sbjct:   44 EELELLRQFDLAWQYGPCTGITRLQRWHRAEQMGLKPPPEVHQVLQS 90

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_MOUSE (DNA polymerase delta subunit 4 OS=Mus musculus GN=Pold4 PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.857e-8
Identity = 28/55 (50.91%), Postives = 36/55 (65.45%), Query Frame = 2

Query:  218 VLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQ---QESLW 373
            +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++LK+       Q SLW
Sbjct:   48 LLRQFDLAWQYGPCTGITRLQRWSRAEQMGLKPPLEVYQVLKAHPEDPHFQCSLW 102

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_HUMAN (DNA polymerase delta subunit 4 OS=Homo sapiens GN=POLD4 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.990e-8
Identity = 26/51 (50.98%), Postives = 34/51 (66.67%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKS 346
            DE      +LRQFD+   YGPC G+TRL RW RA  +GL PP E+ ++LK+
Sbjct:   40 DEEEAELELLRQFDLAWQYGPCTGITRLQRWCRAKQMGLEPPPEVWQVLKT 90

[Close]

BLAST of FG530421 vs. SwissProt
Match: DPOD4_SCHPO (DNA polymerase delta subunit 4 OS=Schizosaccharomyces pombe GN=cdm1 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 4.958e-7
Identity = 22/58 (37.93%), Postives = 36/58 (62.07%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQES 367
            +  ++ E +L  FD    YGP +GMTR+ RW+RA N  LNPP+ + ++L   +  +E+
Sbjct:   88 ENITKVEFILHHFDTTARYGPYLGMTRMQRWKRAKNFNLNPPETVGKILMLEEADEEN 145

[Close]

BLAST of FG530421 vs. Medicago proteins
Match: IMGA|AC235662_11.1 (DNA polymerase delta subunit 4 (AHRD V1 *-** Q9HCU8); contains Interpro domain(s) IPR007218 DNA polymerase delta, subunit 4 AC235662.8 40758-39915 E EGN_Mt100125 20100825)

HSP 1 Score: 105.145 bits (261), Expect = 2.240e-23
Identity = 51/64 (79.69%), Postives = 56/64 (87.50%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            DE + SE+VLRQFDMN  YGPC+GMTRLAR ERAV LGLNPP+EI  LLKSGKVQQESLWD+RI
Sbjct:   51 DEHNGSEAVLRQFDMNMKYGPCMGMTRLARLERAVKLGLNPPEEIAELLKSGKVQQESLWDTRI 114

[Close]

BLAST of FG530421 vs. Medicago proteins
Match: IMGA|AC235662_14.1 (DNA polymerase delta subunit 4 family protein (AHRD V1 *-*- C5P6F7_COCP7); contains Interpro domain(s) IPR007218 DNA polymerase delta, subunit 4 AC235662.8 48885-48543 E EGN_Mt100125 20100825)

HSP 1 Score: 85.1149 bits (209), Expect = 2.399e-17
Identity = 42/65 (64.62%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  194 DECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEI-ERLLKSGKVQQESLWDSRI 385
            DE + SESVLRQFD+N  YGP IG+TRL RWERA    +NPPQEI E +LKSG  QQE LW+ R+
Sbjct:   31 DEQNNSESVLRQFDLNMDYGPSIGLTRLERWERAKKWDMNPPQEIKELILKSGNDQQEGLWNKRV 95

[Close]

BLAST of FG530421 vs. Medicago proteins
Match: IMGA|AC235005_17.1 (DNA polymerase delta subunit 4 (AHRD V1 *-*- Q3T0X9); contains Interpro domain(s) IPR007218 DNA polymerase delta, subunit 4 AC235005.4 65668-66133 H EGN_Mt100125 20100825)

HSP 1 Score: 71.2478 bits (173), Expect = 3.585e-13
Identity = 34/63 (53.97%), Postives = 43/63 (68.25%), Query Frame = 2

Query:  197 ECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            E S+ E  LR+FDMNT YGPC+GMTRL R+ERA   GL PP +I  +LK+  V +E L   R+
Sbjct:   34 EYSKEEMTLREFDMNTAYGPCLGMTRLERYERARKWGLKPPPKIGMILKAFNVDKECLLHDRL 96

[Close]

BLAST of FG530421 vs. Medicago proteins
Match: IMGA|AC235009_14.1 (DNA polymerase delta subunit 4 (AHRD V1 *-*- Q3T0X9); contains Interpro domain(s) IPR007218 DNA polymerase delta, subunit 4 AC235009.1 51052-50587 H EGN_Mt100125 20100825)

HSP 1 Score: 71.2478 bits (173), Expect = 3.585e-13
Identity = 34/63 (53.97%), Postives = 43/63 (68.25%), Query Frame = 2

Query:  197 ECSESESVLRQFDMNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSRI 385
            E S+ E  LR+FDMNT YGPC+GMTRL R+ERA   GL PP +I  +LK+  V +E L   R+
Sbjct:   34 EYSKEEMTLREFDMNTAYGPCLGMTRLERYERARKWGLKPPPKIGMILKAFNVDKECLLHDRL 96

[Close]

BLAST of FG530421 vs. Medicago proteins
Match: IMGA|Medtr5g006580.1 (DNA polymerase delta subunit 4 (AHRD V1 *-*- O59835); contains Interpro domain(s) IPR015270 Protein of unknown function DUF1950 chr05_pseudomolecule_IMGAG_V3.5 755754-754884 H EGN_Mt100125 20100825)

HSP 1 Score: 62.003 bits (149), Expect = 2.175e-10
Identity = 28/49 (57.14%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  236 MNTVYGPCIGMTRLARWERAVNLGLNPPQEIERLLKSGKVQQESLWDSR 382
            M+T YGPC GMTRL R+ERA+   LNPP EI + LK   V +E LW  R
Sbjct:    1 MDTKYGPCFGMTRLERYERALKWNLNPPPEIGKFLKKCNVDEECLWHDR 49

The following BLAST results are available for this feature:

BLAST of FG530421 vs. TrEMBL
BLAST of FG530421 vs. SwissProt
BLAST of FG530421 vs. TAIR peptide
BLAST of FG530421 vs. TAIR peptide
BLAST of FG530421 vs. TrEMBL
BLAST of FG530421 vs. Lotus protein
BLAST of FG530421 vs. Soybean peptides
BLAST of FG530421 vs. SwissProt
BLAST of FG530421 vs. Medicago proteins

BLAST of FG530421 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SDG4_RICCO	1.084e-22	63.77	Delta DNA polymerase, putative OS=Ricinus communis...	[more]
B9IPX7_POPTR	1.243e-20	67.19	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q1H568_ARATH	2.105e-20	65.63	At1g09815 OS=Arabidopsis thaliana GN=At1g09815 PE=...	[more]
Q8L9H3_ARATH	4.638e-20	64.06	Putative uncharacterized protein OS=Arabidopsis th...	[more]
D7KJU8_ARALY	6.726e-19	64.06	DNA polymerase delta subunit 4 family OS=Arabidops...	[more]
D7T7F4_VITVI	7.437e-18	68.85	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AG58_VITVI	7.437e-18	68.85	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A9PI30_POPTR	1.403e-16	64.06	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PA85_POPTR	1.403e-16	64.06	Putative uncharacterized protein OS=Populus tricho...	[more]
A9NT40_PICSI	3.575e-12	51.56	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of FG530421 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
DPOD4_BOVIN	2.582e-8	53.19	DNA polymerase delta subunit 4 OS=Bos taurus GN=PO...	[more]
DPOD4_MOUSE	5.752e-8	50.91	DNA polymerase delta subunit 4 OS=Mus musculus GN=...	[more]
DPOD4_HUMAN	9.812e-8	50.98	DNA polymerase delta subunit 4 OS=Homo sapiens GN=...	[more]
DPOD4_SCHPO	4.870e-7	37.93	DNA polymerase delta subunit 4 OS=Schizosaccharomy...	[more]

BLAST of FG530421 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G09815.1	1.588e-22	65.63	\| Symbols: POLD4 \| polymerase delta 4 \| chr1:31894...	[more]

BLAST of FG530421 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G09815.1	1.588e-22	65.63	\| Symbols: POLD4 \| polymerase delta 4 \| chr1:31894...	[more]

BLAST of FG530421 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SDG4_RICCO	1.325e-22	63.77	Delta DNA polymerase, putative OS=Ricinus communis...	[more]
B9IPX7_POPTR	1.520e-20	67.19	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q1H568_ARATH	2.574e-20	65.63	At1g09815 OS=Arabidopsis thaliana GN=At1g09815 PE=...	[more]
Q8L9H3_ARATH	5.671e-20	64.06	Putative uncharacterized protein OS=Arabidopsis th...	[more]
D7KJU8_ARALY	8.223e-19	64.06	DNA polymerase delta subunit 4 family OS=Arabidops...	[more]
D7T7F4_VITVI	9.091e-18	68.85	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AG58_VITVI	9.091e-18	68.85	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A9PI30_POPTR	1.715e-16	64.06	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PA85_POPTR	1.715e-16	64.06	Putative uncharacterized protein OS=Populus tricho...	[more]
A9NT40_PICSI	4.370e-12	51.56	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of FG530421 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1


Match Name	E-value	Identity	Description
chr4.CM0337.20.r2.d	4.377e-23	76.56	- phase: 0	[more]

BLAST of FG530421 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2


Match Name	E-value	Identity	Description
Glyma05g25400.1\|PACid:16259574	1.008e-27	79.69		[more]
Glyma08g08420.1\|PACid:16270411	1.256e-24	79.69		[more]

BLAST of FG530421 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
DPOD4_BOVIN	2.629e-8	53.19	DNA polymerase delta subunit 4 OS=Bos taurus GN=PO...	[more]
DPOD4_MOUSE	5.857e-8	50.91	DNA polymerase delta subunit 4 OS=Mus musculus GN=...	[more]
DPOD4_HUMAN	9.990e-8	50.98	DNA polymerase delta subunit 4 OS=Homo sapiens GN=...	[more]
DPOD4_SCHPO	4.958e-7	37.93	DNA polymerase delta subunit 4 OS=Schizosaccharomy...	[more]

BLAST of FG530421 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 5


Match Name	E-value	Identity	Description
IMGA\|AC235662_11.1	2.240e-23	79.69	DNA polymerase delta subunit 4 (AHRD V1 -* Q9HCU...	[more]
IMGA\|AC235662_14.1	2.399e-17	64.62	DNA polymerase delta subunit 4 family protein (AHR...	[more]
IMGA\|AC235005_17.1	3.585e-13	53.97	DNA polymerase delta subunit 4 (AHRD V1 -- Q3T0X...	[more]
IMGA\|AC235009_14.1	3.585e-13	53.97	DNA polymerase delta subunit 4 (AHRD V1 -- Q3T0X...	[more]
IMGA\|Medtr5g006580.1	2.175e-10	57.14	DNA polymerase delta subunit 4 (AHRD V1 -- O5983...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007218	DNA polymerase delta, subunit 4	PFAM	PF04081	DNA_pol_delta_4	coord: 3..57 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 36..47 score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007218	DNA polymerase delta, subunit 4	PFAM	PF04081	DNA_pol_delta_4	coord: 3..57 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 36..47 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG530421 ID=FG530421; Name=FG530421; organism=Pisum sativum; type=EST; length=564bp
ACACCAAAAAATCTCCTATCCACGCCGCCACTTTCAGTTCCAATCTCGCC
CAACCACCAGCCCTCACATCCCGCAACGGCAAACCAGATCTCCAAGGTTC
TAATTTTTTCATTCTTTTAATTTCTAGGGTTTTCTCATTCACCCAAATCA
CTTCATTTTTTTCGGTAATCTGATTTGTGCTTTGTGGATTTAAGATGAAT
GTAGCGAAAGCGAGTCGGTGTTGCGCCAATTTGACATGAACACGGTTTAC
GGTCCTTGCATTGGGATGACGAGGCTGGCACGCTGGGAGCGTGCGGTGAA
TCTCGGTTTGAACCCTCCGCAGGAAATTGAGAGGCTTTTGAAAAGTGGTA
AAGTTCAGCAAGAATCCCTCTGGGATAGTCGCATTTAGAATGCTTTTGTG
TGTTTTATTATTATCATTATAACCTATAATCAAAGTGTAATCTTTGTGTT
TAGATTTTGCTTAGGGAAATCAGTTTCTGGCTGTGTACCATTTCCCATGC
TACTGTATTTCGTTAACTTGGTGATGCTAAGGTGTTAAGTGAATGCTTAT
GCTATTACTATATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication

Vocabulary: **INTERPRO**
Term	Definition
IPR007218	DNA_pol_delta_4

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