FG531835

EST Overview


Name	FG531835
Unique Name	FG531835
Type	EST
Organism	Pisum sativum (pea)
Sequence length	416

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG531835 vs. TrEMBL
Match: Q45FE6_MEDTR (Serine hydroxymethyltransferase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 1.783e-66
Identity = 129/138 (93.48%), Postives = 136/138 (98.55%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQAMTPEFK+YQKQVLSNSSTFAQSL+E+GYDLVSGGTENHLVLVNLR+KGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF++VAEYFDAAVKIALQI+ NSKGTK
Sbjct:  324 NHTITGLAVALKQAMTPEFKNYQKQVLSNSSTFAQSLLEKGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEDDFKKVAEYFDAAVKIALQIKENSKGTK 461

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BLAST of FG531835 vs. TrEMBL
Match: B9SMX7_RICCO (Serine hydroxymethyltransferase OS=Ricinus communis GN=RCOM_0482360 PE=3 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.479e-60
Identity = 115/138 (83.33%), Postives = 131/138 (94.93%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI+GLAVALKQ MTPE+K+YQ+QVL N S F+QSL+E+GY+LVSGGTENHLVLVNLR+KGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAE+FDAAVK+AL+I+ ++KGTK
Sbjct:  331 NHTISGLAVALKQVMTPEYKAYQEQVLKNCSKFSQSLLEKGYELVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVKLALKIKADTKGTK 468

[Close]

BLAST of FG531835 vs. TrEMBL
Match: A9PL06_POPTM (Serine hydroxymethyltransferase OS=Populus tremuloides GN=SHMT3 PE=2 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 3.602e-59
Identity = 115/138 (83.33%), Postives = 131/138 (94.93%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI+GLAVALKQA TPE+K+YQ+QVLSN S FAQSLIE+GY+LVSGGTENHLVLVNLR+KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VAE+FDAAVK+AL+I+ +++GTK
Sbjct:  334 NHTISGLAVALKQARTPEYKAYQEQVLSNCSKFAQSLIEKGYELVSGGTENHLVLVNLRNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIERDFVKVAEFFDAAVKLALKIKADAQGTK 471

[Close]

BLAST of FG531835 vs. TrEMBL
Match: C6ZJZ0_SOYBN (Serine hydroxymethyltransferase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.144e-59
Identity = 115/138 (83.33%), Postives = 130/138 (94.20%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+++YQ+QVLSNS  FAQ+L ERGY+LVSGGTENHLVLVNL++KGIDGSRVEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAE+FDAAVK+A++I+  SKGTK
Sbjct:  335 NHTITGLAVALKQATTPEYRAYQEQVLSNSFKFAQALSERGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEFFDAAVKLAVKIKGQSKGTK 472

[Close]

BLAST of FG531835 vs. TrEMBL
Match: B2BGS6_OLEEU (Glycine/serine hydroxymethyltransferase reductase (Fragment) OS=Olea europaea PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.144e-59
Identity = 113/138 (81.88%), Postives = 131/138 (94.93%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+K+YQ+QV+SN S FAQ+L+++ YDLVSGGTENHLVLVNL++KGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAE+FDA+VK+AL+I+ N++GTK
Sbjct:   20 NHTITGLAVALKQATTPEYKAYQEQVMSNCSKFAQTLVKKSYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEFFDASVKLALKIKANTQGTK 157

[Close]

BLAST of FG531835 vs. TrEMBL
Match: A9YWS0_MEDTR (Serine hydroxymethyltransferase OS=Medicago truncatula PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.369e-58
Identity = 114/138 (82.61%), Postives = 129/138 (93.48%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+K+YQ+QVLSN + FAQ+L E+GY+LVSGGTENHLVLVNL++KGIDGSRVEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAEYFDA+V +AL+I+  SKGTK
Sbjct:  335 NHTITGLAVALKQATTPEYKAYQEQVLSNCAKFAQALSEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDASVNLALKIKAESKGTK 472

[Close]

BLAST of FG531835 vs. TrEMBL
Match: C6ZJY8_SOYBN (Serine hydroxymethyltransferase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.788e-58
Identity = 115/138 (83.33%), Postives = 129/138 (93.48%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+++YQ+QVLSNS  FAQ+L ER Y+LVSGGTENHLVLVNL++KGIDGSRVEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAE+FDAAVKIA++I+  SKGTK
Sbjct:  333 NHTITGLAVALKQATTPEYRAYQEQVLSNSFKFAQALSERSYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEFFDAAVKIAVKIKGESKGTK 470

[Close]

BLAST of FG531835 vs. TrEMBL
Match: B9GN69_POPTR (Serine hydroxymethyltransferase OS=Populus trichocarpa GN=SHMT3 PE=3 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.788e-58
Identity = 114/138 (82.61%), Postives = 130/138 (94.20%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI+GLAVALKQA TPE+K+YQ+QVLSN S FAQSLIE+GY+LVSGGTENHLVLVNLR+KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VAE+FDAAVK+AL+I+ +++G K
Sbjct:  334 NHTISGLAVALKQARTPEYKAYQEQVLSNCSKFAQSLIEKGYELVSGGTENHLVLVNLRNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIERDFVKVAEFFDAAVKLALKIKADAQGMK 471

[Close]

BLAST of FG531835 vs. TrEMBL
Match: B9HV02_POPTR (Serine hydroxymethyltransferase OS=Populus trichocarpa GN=SHMT7 PE=3 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.793e-58
Identity = 112/138 (81.16%), Postives = 129/138 (93.48%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI GLAVALKQA T E+K+YQ+QVLSN + FAQSL+E+GY+LVSGGTENHLVLVNL++KGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VA++FDAAVK+A++I+  +KGTK
Sbjct:  338 NHTIAGLAVALKQATTVEYKAYQEQVLSNCAKFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVADFFDAAVKLAVEIKAETKGTK 475

[Close]

BLAST of FG531835 vs. TrEMBL
Match: A9PL10_POPTM (Serine hydroxymethyltransferase OS=Populus tremuloides GN=SHMT7 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.793e-58
Identity = 112/138 (81.16%), Postives = 129/138 (93.48%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI GLAVALKQA T E+K+YQ+QVLSN + FAQSL+E+GY+LVSGGTENHLVLVNL++KGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VA++FDAAVK+A++I+  +KGTK
Sbjct:  338 NHTIAGLAVALKQATTVEYKAYQEQVLSNCAKFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVADFFDAAVKVAVKIKAETKGTK 475

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BLAST of FG531835 vs. SwissProt
Match: GLYM_PEA (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 2.319e-60
Identity = 115/138 (83.33%), Postives = 129/138 (93.48%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+++YQ+QVLSNSS FA++L E+GYDLVSGGTENHLVLVNL++KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAEYFDAAV +AL+++  SKGTK
Sbjct:  335 NHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTK 472

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_SOLTU (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 2.170e-58
Identity = 109/138 (78.99%), Postives = 131/138 (94.93%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+++YQ+QVLSNSS FAQ+L E+GY+LVSGGT+NHLVLVN+++KGIDGSRVEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VA++FDAAVKIA++++  ++GTK
Sbjct:  335 NHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTK 472

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 2.170e-58
Identity = 111/138 (80.43%), Postives = 128/138 (92.75%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA T E+K+YQ+QVLSNS+ FAQ+L+ERGY+LVSGGT+NHLVLVNL+ KGIDGSRVEKVLE+VHIA+NKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAEYFD AV IAL+++  ++GTK
Sbjct:  334 NHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTK 471

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_FLAPR (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 6.981e-57
Identity = 107/138 (77.54%), Postives = 128/138 (92.75%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA T E+K+YQ+QV+SNS+ FA++L++ GY+LVSGGTENHLVLVNL++KGIDGS+VEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VA +FD AVK+A++I+  +KGTK
Sbjct:  335 NHTITGLAVALKQATTAEYKAYQEQVMSNSAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDLAVKLAVKIKGEAKGTK 472

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 1.008e-55
Identity = 106/138 (76.81%), Postives = 126/138 (91.30%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA T E+K+YQ+QV+SN + FA++L++ GY+LVSGGTENHLVLVNL++KGIDGSRVEKVLE+VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VA  FD AVK+A++I+  ++GTK
Sbjct:  335 NHTITGLAVALKQATTAEYKAYQEQVMSNCAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYLFDLAVKLAVKIKGEAQGTK 472

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_CANGA (Serine hydroxymethyltransferase, mitochondrial OS=Candida glabrata GN=SHM1 PE=3 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 1.366e-44
Identity = 81/131 (61.83%), Postives = 110/131 (83.97%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR 394
            NHTI+ LAVALKQA TPEF  YQKQV+SN+  F   L++RG++LVSGGT+NHL+L+NL + GIDG+R+E +LE ++IAANKNT+PGD SA+ P G+R+GTPA+T+RGF E DF++VAEY D AVK+++ ++
Sbjct:  308 NHTISALAVALKQAKTPEFVEYQKQVVSNAKAFGDELLKRGFELVSGGTDNHLLLLNLSNMGIDGARLEAILEKINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFQEQDFKKVAEYIDNAVKLSIALK 438

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYC_SCHPO (Probable serine hydroxymethyltransferase, cytosolic OS=Schizosaccharomyces pombe GN=shm2 PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 1.669e-42
Identity = 84/130 (64.62%), Postives = 101/130 (77.69%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQI 391
            NHTIT LAVAL QA TPEF  YQK VLSN+   A + I RGY LVSGGT+ HLVLV+L DKG+DG+RVE++LE V+I+ANKNTVPGD SA++P G+R+GTPA T+RGF E DFERV E  D  V +  +I
Sbjct:  297 NHTITALAVALGQAKTPEFYQYQKDVLSNAKAMANAFITRGYKLVSGGTDTHLVLVDLTDKGVDGARVERILELVNISANKNTVPGDKSALIPRGLRLGTPACTTRGFDEKDFERVVELIDEVVSLTKKI 426

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_NEUCR (Putative serine hydroxymethyltransferase, mitochondrial OS=Neurospora crassa GN=NCU05805 PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.719e-42
Identity = 86/136 (63.24%), Postives = 107/136 (78.68%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIER------GYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQI 391
            NHTI  LAVALKQA TPEF++YQ QVL+N++  A  L E       GY +VSGGT+NHLVL++L+ +GIDGSRVE+VLE V +AANKNTVPGD SA+ PGG+R+GTPA+T+RGF E DF RVA+  D AV IA++I
Sbjct:  334 NHTIAALAVALKQAQTPEFRAYQSQVLANATALAARLGEPKDKNGLGYTIVSGGTDNHLVLIDLKPQGIDGSRVERVLELVGVAANKNTVPGDKSALTPGGLRIGTPAMTTRGFTEEDFARVADIIDRAVTIAVRI 469

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_BOVIN (Serine hydroxymethyltransferase, mitochondrial OS=Bos taurus GN=SHMT2 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 6.344e-42
Identity = 83/138 (60.14%), Postives = 103/138 (74.64%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NH I  +AVALKQA TP F+ Y  Q+L N+   A +L+ERGY LVSGGT+NHLVLV+LR KG+DG+R E+VLE V I ANKNT PGD SA+ PGG+R+G PALTSRGF+E DF +V  + D  V I L+++  SK TK
Sbjct:  329 NHAIAAVAVALKQACTPMFREYSLQILKNAQAMANALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRGFLEDDFRKVVGFIDEGVNIGLEVK--SKTTK 464

[Close]

BLAST of FG531835 vs. SwissProt
Match: GLYM_KLULA (Serine hydroxymethyltransferase, mitochondrial OS=Kluyveromyces lactis GN=SHM1 PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 4.112e-41
Identity = 76/131 (58.02%), Postives = 106/131 (80.92%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR 394
            NHTI+ LAVALKQA TPEFK YQ  V+ N+  F + L+++G++LVSGGT+ HL+L+NL + GIDG+R+E +LE+++IAANKNT+PGD SA+ P G+R+GTPA+T+RGF   +F +VA Y D AVK+A+ I+
Sbjct:  321 NHTISALAVALKQAATPEFKEYQAAVVENARIFGEELVKKGFELVSGGTDTHLILINLSNLGIDGARLETLLENINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFGPQEFAQVAAYIDRAVKLAIGIK 451

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT5G26780.1 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=517)

HSP 1 Score: 224.942 bits (572), Expect = 1.163e-59
Identity = 112/138 (81.16%), Postives = 126/138 (91.30%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+K+YQ QVL N S FA++L+ +GYDLVSGGT+NHLVLVNL++KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VAEYFD AVKIAL+I+  S+GTK
Sbjct:  334 NHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTK 471

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT4G37930.1 (| Symbols: SHM1, STM, SHMT1 | serine transhydroxymethyltransferase 1 | chr4:17831891-17834742 REVERSE LENGTH=517)

HSP 1 Score: 223.787 bits (569), Expect = 2.591e-59
Identity = 111/138 (80.43%), Postives = 128/138 (92.75%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA T E+K+YQ+QVLSNS+ FAQ+L+ERGY+LVSGGT+NHLVLVNL+ KGIDGSRVEKVLE+VHIA+NKNTVPGDVSAMVPGGIRMGTPALTSRGFVE DF +VAEYFD AV IAL+++  ++GTK
Sbjct:  334 NHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTK 471

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT5G26780.2 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 215.698 bits (548), Expect = 7.056e-57
Identity = 113/154 (73.38%), Postives = 126/154 (81.82%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFA----------------QSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+K+YQ QVL N S FA                Q+L+ +GYDLVSGGT+NHLVLVNL++KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VAEYFD AVKIAL+I+  S+GTK
Sbjct:  334 NHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTK 487

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT5G26780.3 (| Symbols: SHM2 | serine hydroxymethyltransferase 2 | chr5:9418299-9421725 FORWARD LENGTH=533)

HSP 1 Score: 215.698 bits (548), Expect = 7.056e-57
Identity = 113/154 (73.38%), Postives = 126/154 (81.82%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFA----------------QSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTITGLAVALKQA TPE+K+YQ QVL N S FA                Q+L+ +GYDLVSGGT+NHLVLVNL++KGIDGSRVEKVLE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF +VAEYFD AVKIAL+I+  S+GTK
Sbjct:  334 NHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTK 487

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT4G32520.2 (| Symbols: SHM3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 159.073 bits (401), Expect = 7.838e-40
Identity = 77/138 (55.80%), Postives = 104/138 (75.36%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI GLAV LK A +PEFK+YQK+V+SN    A  L+E G+ LVSGG++NHLVLV+LR  G+DG+RVEK+L+   I  NKN+VPGD SA+VPGGIR+G+PA+T+RG  E DF  VA++    V+I ++ +  + G+K
Sbjct:  354 NHTIGGLAVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSK 491

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT4G32520.1 (| Symbols: SHM3, AtSHMT3 | serine hydroxymethyltransferase 3 | chr4:15689642-15692334 REVERSE LENGTH=529)

HSP 1 Score: 159.073 bits (401), Expect = 7.838e-40
Identity = 77/138 (55.80%), Postives = 104/138 (75.36%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR*NSKGTK 415
            NHTI GLAV LK A +PEFK+YQK+V+SN    A  L+E G+ LVSGG++NHLVLV+LR  G+DG+RVEK+L+   I  NKN+VPGD SA+VPGGIR+G+PA+T+RG  E DF  VA++    V+I ++ +  + G+K
Sbjct:  354 NHTIGGLAVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSK 491

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT4G13890.1 (| Symbols: SHM5, EDA36, EDA37 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr4:8032146-8033718 REVERSE LENGTH=470)

HSP 1 Score: 141.739 bits (356), Expect = 1.295e-34
Identity = 70/131 (53.44%), Postives = 92/131 (70.23%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR 394
            N+ I  LAVALKQ M P FK Y KQV +N++  A  LI +GY LV+ GT+NHL+L +LR  G+ G++VEKV E  +I  N+N V GD S + PGG+R+GTPA+TSRG VE DFE++ E+   AV I L I+
Sbjct:  293 NNKIGALAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITLDIQ 423

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT4G13930.1 (| Symbols: SHM4 | serine hydroxymethyltransferase 4 | chr4:8048013-8050021 REVERSE LENGTH=471)

HSP 1 Score: 139.043 bits (349), Expect = 8.393e-34
Identity = 69/131 (52.67%), Postives = 90/131 (68.70%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIALQIR 394
            NH I  LAVALKQA TP FK Y KQV +N+      L+ +GY +V+ GTENHLVL +LR  G+ G++VEK+ +   I  NKN V GD SA+ PGG+R+G PA+TSRG VE DFE++ E+   AV + L I+
Sbjct:  293 NHQIGALAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTLDIQ 423

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT1G36370.1 (| Symbols: SHM7 | serine hydroxymethyltransferase 7 | chr1:13696240-13698576 REVERSE LENGTH=598)

HSP 1 Score: 119.398 bits (298), Expect = 6.882e-28
Identity = 58/126 (46.03%), Postives = 83/126 (65.87%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKI 379
            N+ I  LA+ALKQ  TPE+K+Y +Q+  N+   A +L+ R   LV+GGT+NHL+L +L   G+ G   EKV E  HI  NK  + GD   + PGG+R+GTPA+T+RG +E DFE +A++   A +I
Sbjct:  424 NNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

[Close]

BLAST of FG531835 vs. TAIR peptide
Match: AT1G22020.1 (| Symbols: SHM6 | serine hydroxymethyltransferase 6 | chr1:7754599-7757087 FORWARD LENGTH=599)

HSP 1 Score: 115.161 bits (287), Expect = 1.298e-26
Identity = 57/127 (44.88%), Postives = 83/127 (65.35%), Query Frame = 2

Query:    2 NHTITGLAVALKQAMTPEFKSYQKQVLSNSSTFAQSLIERGYDLVSGGTENHLVLVNLRDKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEGDFERVAEYFDAAVKIA 382
            N+ I  LA+ALKQA +PE+K Y +QV  N+   A +LI R   L++GGT+NHL+L +L   G+ G   EKV E  HI  NK  +  +   + PGG+R+G+PA+TSRG +E +FE +A++   A +IA
Sbjct:  428 NNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQIA 554

The following BLAST results are available for this feature:

BLAST of FG531835 vs. TrEMBL
BLAST of FG531835 vs. SwissProt
BLAST of FG531835 vs. TAIR peptide

BLAST of FG531835 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q45FE6_MEDTR	1.783e-66	93.48	Serine hydroxymethyltransferase OS=Medicago trunca...	[more]
B9SMX7_RICCO	9.479e-60	83.33	Serine hydroxymethyltransferase OS=Ricinus communi...	[more]
A9PL06_POPTM	3.602e-59	83.33	Serine hydroxymethyltransferase OS=Populus tremulo...	[more]
C6ZJZ0_SOYBN	6.144e-59	83.33	Serine hydroxymethyltransferase OS=Glycine max PE=...	[more]
B2BGS6_OLEEU	6.144e-59	81.88	Glycine/serine hydroxymethyltransferase reductase ...	[more]
A9YWS0_MEDTR	1.369e-58	82.61	Serine hydroxymethyltransferase OS=Medicago trunca...	[more]
C6ZJY8_SOYBN	1.788e-58	83.33	Serine hydroxymethyltransferase OS=Glycine max PE=...	[more]
B9GN69_POPTR	1.788e-58	82.61	Serine hydroxymethyltransferase OS=Populus trichoc...	[more]
B9HV02_POPTR	6.793e-58	81.16	Serine hydroxymethyltransferase OS=Populus trichoc...	[more]
A9PL10_POPTM	6.793e-58	81.16	Serine hydroxymethyltransferase OS=Populus tremulo...	[more]

BLAST of FG531835 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GLYM_PEA	2.319e-60	83.33	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_SOLTU	2.170e-58	78.99	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_ARATH	2.170e-58	80.43	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_FLAPR	6.981e-57	77.54	Serine hydroxymethyltransferase 1, mitochondrial O...	[more]
GLYN_FLAPR	1.008e-55	76.81	Serine hydroxymethyltransferase 2, mitochondrial O...	[more]
GLYM_CANGA	1.366e-44	61.83	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYC_SCHPO	1.669e-42	64.62	Probable serine hydroxymethyltransferase, cytosoli...	[more]
GLYM_NEUCR	3.719e-42	63.24	Putative serine hydroxymethyltransferase, mitochon...	[more]
GLYM_BOVIN	6.344e-42	60.14	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]
GLYM_KLULA	4.112e-41	58.02	Serine hydroxymethyltransferase, mitochondrial OS=...	[more]

BLAST of FG531835 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G26780.1	1.163e-59	81.16	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT4G37930.1	2.591e-59	80.43	\| Symbols: SHM1, STM, SHMT1 \| serine transhydroxym...	[more]
AT5G26780.2	7.056e-57	73.38	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT5G26780.3	7.056e-57	73.38	\| Symbols: SHM2 \| serine hydroxymethyltransferase ...	[more]
AT4G32520.2	7.838e-40	55.80	\| Symbols: SHM3 \| serine hydroxymethyltransferase ...	[more]
AT4G32520.1	7.838e-40	55.80	\| Symbols: SHM3, AtSHMT3 \| serine hydroxymethyltra...	[more]
AT4G13890.1	1.295e-34	53.44	\| Symbols: SHM5, EDA36, EDA37 \| Pyridoxal phosphat...	[more]
AT4G13930.1	8.393e-34	52.67	\| Symbols: SHM4 \| serine hydroxymethyltransferase ...	[more]
AT1G36370.1	6.882e-28	46.03	\| Symbols: SHM7 \| serine hydroxymethyltransferase ...	[more]
AT1G22020.1	1.298e-26	44.88	\| Symbols: SHM6 \| serine hydroxymethyltransferase ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 1..130 score: 9
IPR001085	Serine hydroxymethyltransferase	PFAM	PF00464	SHMT	coord: 1..119 score: 5.1
IPR015422	Pyridoxal phosphate-dependent transferase, major region, subdomain 2	GENE3D	3.90.1150.10	no description	coord: 16..130 score: 2.8
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 1..124 score: 2.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531835 ID=FG531835; Name=FG531835; organism=Pisum sativum; type=EST; length=416bp
TAATCACACTATTACAGGCCTAGCAGTTGCATTAAAGCAGGCTATGACAC
CAGAGTTCAAGAGTTATCAGAAACAAGTTCTTAGTAACTCCTCGACTTTT
GCACAGAGTTTGATAGAGAGAGGCTATGACCTTGTATCTGGCGGAACTGA
GAACCATTTGGTGTTAGTGAACTTAAGAGACAAGGGCATTGATGGCTCCA
GGGTTGAGAAGGTGTTGGAATCAGTTCATATAGCTGCCAATAAAAATACT
GTTCCAGGGGATGTGTCTGCTATGGTTCCAGGGGGTATCAGAATGGGAAC
CCCTGCTCTCACATCCAGGGGTTTTGTTGAGGGTGATTTTGAGAGAGTAG
CTGAATACTTTGACGCTGCAGTCAAAATAGCCTTACAGATCAGATGAAAT
TCTAAAGGCACAAAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004372	glycine hydroxymethyltransferase activity
GO:0003824	catalytic activity
GO:0030170	pyridoxal phosphate binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006544	glycine metabolic process
GO:0006563	L-serine metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001085	Ser_HO-MeTrfase
IPR015422	PyrdxlP-dep_Trfase_small
IPR015424	PyrdxlP-dep_Trfase

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