FG536092

EST Overview


Name	FG536092
Unique Name	FG536092
Type	EST
Organism	Pisum sativum (pea)
Sequence length	502

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B7FGB8_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.292e-27
Identity = 62/85 (72.94%), Postives = 64/85 (75.29%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGSQS 256
            GMAATVCWISNLIVSESFLSIAD IG+  TF                YVPETQGLTFDEVELIWKERAWGKNP TQ+LLE GSQS
Sbjct:  120 GMAATVCWISNLIVSESFLSIADAIGIASTFLIIAVIAVVAFLFVLLYVPETQGLTFDEVELIWKERAWGKNPNTQNLLEQGSQS 204

[Close]

BLAST of FG536092 vs. TrEMBL
Match: A2Q2S7_MEDTR (General substrate transporter OS=Medicago truncatula GN=MtrDRAFT_AC152184g3v2 PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.292e-27
Identity = 62/85 (72.94%), Postives = 64/85 (75.29%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGSQS 256
            GMAATVCWISNLIVSESFLSIAD IG+  TF                YVPETQGLTFDEVELIWKERAWGKNP TQ+LLE GSQS
Sbjct:  416 GMAATVCWISNLIVSESFLSIADAIGIASTFLIIAVIAVVAFLFVLLYVPETQGLTFDEVELIWKERAWGKNPNTQNLLEQGSQS 500

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B9SUS6_RICCO (Sugar transporter, putative OS=Ricinus communis GN=RCOM_0573930 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.189e-17
Identity = 45/85 (52.94%), Postives = 59/85 (69.41%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGSQS 256
            GM+ATV WISNLIV+++FLS+A+ +G G TF                +VPET+GLTF EVE IWKERAWG +  T+SLLE G+++
Sbjct:  223 GMSATVNWISNLIVAQTFLSVANAVGTGATFLILAGVAIAAIVFIILFVPETKGLTFVEVEQIWKERAWGSSYNTESLLERGNET 307

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B9HH72_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_832210 PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.553e-17
Identity = 47/85 (55.29%), Postives = 56/85 (65.88%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGSQS 256
            GM+ATV WISNLIV+E+FLS+A+ +G G TF                YVPET GLTF EVE IWKERAWG    T+SLLE G+ +
Sbjct:  203 GMSATVNWISNLIVAETFLSVAEAVGTGWTFVILACIAVLAFVFVIVYVPETVGLTFVEVEQIWKERAWGSGYNTESLLERGNDN 287

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B9NEI7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_680198 PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.650e-17
Identity = 46/83 (55.42%), Postives = 55/83 (66.27%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGS 250
            GM+ATV WISNLIV+++FLSIA+ +G G TF                YVPET GL F EVE IWKERAWG +  T+SLLE G+
Sbjct:  223 GMSATVNWISNLIVAQTFLSIAEAVGTGSTFLMLAGIAVLAVVFVIMYVPETMGLAFVEVEQIWKERAWGSSYNTESLLEQGN 305

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B9MY11_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_292630 PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.650e-17
Identity = 46/83 (55.42%), Postives = 55/83 (66.27%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGS 250
            GM+ATV WISNLIV+++FLSIA+ +G G TF                YVPET GL F EVE IWKERAWG +  T+SLLE G+
Sbjct:  407 GMSATVNWISNLIVAQTFLSIAEAVGTGSTFLMLAGIAVLAVVFVIMYVPETMGLAFVEVEQIWKERAWGSSYNTESLLEQGN 489

[Close]

BLAST of FG536092 vs. TrEMBL
Match: Q84UY4_MESCR (Putative Na+/myo-inositol symporter OS=Mesembryanthemum crystallinum GN=Itr3 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 8.524e-16
Identity = 43/85 (50.59%), Postives = 55/85 (64.71%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGSQS 256
            GM AT+CWI NL VSE+FLSIAD IG GPTF                +VPET+ LTF+EV+ ++ +RA+G    TQSLLE  ++S
Sbjct:  412 GMGATICWIVNLFVSETFLSIADAIGTGPTFLIIAGIVIVAFVFVVCFVPETKALTFEEVDQMFMDRAYGTEENTQSLLESSNRS 496

[Close]

BLAST of FG536092 vs. TrEMBL
Match: B9NEI9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_680200 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.899e-15
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLLEHGS 250
            GM+ATV WISNLIV+++FLSIA+ +G G TF                YVPET GL F EVE I KERAWG +  T+SLL+ G+
Sbjct:  112 GMSATVNWISNLIVAQTFLSIAEAVGTGSTFLMLAGTAVLAVVFVIMYVPETMGLAFVEVEQIGKERAWGSSYNTESLLDQGN 194

[Close]

BLAST of FG536092 vs. TrEMBL
Match: Q7XUE4_ORYSJ (OSJNBa0076N16.21 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0076N16.21 PE=2 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 7.216e-15
Identity = 41/79 (51.90%), Postives = 52/79 (65.82%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLL 238
            GM+ATV W+SNLIV+++FLSI   +G G TF                YVPET+GL+F++VEL+WKERAWG     QSLL
Sbjct:  424 GMSATVNWVSNLIVAQTFLSIVGLVGTGLTFLIIAGIAVLAFIFVALYVPETKGLSFEQVELLWKERAWGNQGNRQSLL 502

[Close]

BLAST of FG536092 vs. TrEMBL
Match: C5YBD4_SORBI (Putative uncharacterized protein Sb06g021070 OS=Sorghum bicolor GN=Sb06g021070 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.216e-15
Identity = 41/79 (51.90%), Postives = 52/79 (65.82%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERAWGKNPITQSLL 238
            GM+ATV WISNLIV+++FLSI   +G GPTF                YVPET+GL+F++VE +WKERAWG +   Q LL
Sbjct:  423 GMSATVNWISNLIVAQTFLSIVGLVGTGPTFLIIAGIAVLAFIFVAMYVPETKGLSFEQVEQMWKERAWGNSGNCQRLL 501

[Close]

BLAST of FG536092 vs. SwissProt
Match: INT1_ARATH (Probable inositol transporter 1 OS=Arabidopsis thaliana GN=INT1 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.290e-15
Identity = 47/94 (50.00%), Postives = 57/94 (60.64%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERA------WGKNPIT---QSLLEHGSQS 256
            GM+ATV WISNLIV+++FL+IA+  G G TF                +VPETQGLTF EVE IWKERA      WG +  +   + LLE GSQS
Sbjct:  416 GMSATVNWISNLIVAQTFLTIAEAAGTGMTFLILAGIAVLAVIFVIVFVPETQGLTFSEVEQIWKERAYGNISGWGSSSDSNNMEGLLEQGSQS 509

[Close]

BLAST of FG536092 vs. SwissProt
Match: INT2_ARATH (Probable inositol transporter 2 OS=Arabidopsis thaliana GN=INT2 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 6.417e-7
Identity = 33/90 (36.67%), Postives = 47/90 (52.22%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERA-----WGKNPITQSLLEHGSQS 256
            G+AAT  WISNLIV++SFLS+ + IG   TF                 VPET+G+  +E+E + + R+     W K      L+E  +QS
Sbjct:  493 GIAATANWISNLIVAQSFLSLTEAIGTSWTFLIFGVISVIALLFVMVCVPETKGMPMEEIEKMLERRSMEFKFWKKK---SKLVEKQNQS 579

[Close]

BLAST of FG536092 vs. TAIR peptide
Match: AT2G43330.1 (| Symbols: ATINT1, INT1 | inositol transporter 1 | chr2:18001135-18003854 FORWARD LENGTH=509)

HSP 1 Score: 82.4185 bits (202), Expect = 1.424e-16
Identity = 47/94 (50.00%), Postives = 57/94 (60.64%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERA------WGKNPIT---QSLLEHGSQS 256
            GM+ATV WISNLIV+++FL+IA+  G G TF                +VPETQGLTF EVE IWKERA      WG +  +   + LLE GSQS
Sbjct:  416 GMSATVNWISNLIVAQTFLTIAEAAGTGMTFLILAGIAVLAVIFVIVFVPETQGLTFSEVEQIWKERAYGNISGWGSSSDSNNMEGLLEQGSQS 509

[Close]

BLAST of FG536092 vs. TAIR peptide
Match: AT1G30220.1 (| Symbols: ATINT2, INT2 | inositol transporter 2 | chr1:10632957-10635439 REVERSE LENGTH=580)

HSP 1 Score: 53.5286 bits (127), Expect = 7.083e-8
Identity = 33/90 (36.67%), Postives = 47/90 (52.22%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVELIWKERA-----WGKNPITQSLLEHGSQS 256
            G+AAT  WISNLIV++SFLS+ + IG   TF                 VPET+G+  +E+E + + R+     W K      L+E  +QS
Sbjct:  493 GIAATANWISNLIVAQSFLSLTEAIGTSWTFLIFGVISVIALLFVMVCVPETKGMPMEEIEKMLERRSMEFKFWKKK---SKLVEKQNQS 579

[Close]

BLAST of FG536092 vs. TAIR peptide
Match: AT4G16480.1 (| Symbols: ATINT4, INT4 | inositol transporter 4 | chr4:9291246-9293083 FORWARD LENGTH=582)

HSP 1 Score: 52.7582 bits (125), Expect = 1.208e-7
Identity = 28/61 (45.90%), Postives = 34/61 (55.74%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVE 184
            G+AA   W+SNLIVSESFLS+   +G   TF                 VPET+GL F+EVE
Sbjct:  497 GIAAVSNWVSNLIVSESFLSLTHALGSSGTFLLFAGFSTIGLFFIWLLVPETKGLQFEEVE 557

[Close]

BLAST of FG536092 vs. TAIR peptide
Match: AT2G35740.1 (| Symbols: ATINT3, INT3 | nositol transporter 3 | chr2:15024489-15026414 REVERSE LENGTH=580)

HSP 1 Score: 50.447 bits (119), Expect = 5.996e-7
Identity = 25/61 (40.98%), Postives = 35/61 (57.38%), Query Frame = 2

Query:    2 GMAATVCWISNLIVSESFLSIADGIGVGPTFXXXXXXXXXXXXXXXXYVPETQGLTFDEVE 184
            G+AA   W+SNL+VSE+FL++ + +G   TF                 VPET+GL F+EVE
Sbjct:  496 GIAAVSNWMSNLVVSETFLTLTNAVGSSGTFLLFAGSSAVGLFFIWLLVPETKGLQFEEVE 556

The following BLAST results are available for this feature:

BLAST of FG536092 vs. TrEMBL
BLAST of FG536092 vs. SwissProt
BLAST of FG536092 vs. TAIR peptide

BLAST of FG536092 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FGB8_MEDTR	6.292e-27	72.94	Putative uncharacterized protein OS=Medicago trunc...	[more]
A2Q2S7_MEDTR	6.292e-27	72.94	General substrate transporter OS=Medicago truncatu...	[more]
B9SUS6_RICCO	1.189e-17	52.94	Sugar transporter, putative OS=Ricinus communis GN...	[more]
B9HH72_POPTR	1.553e-17	55.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9NEI7_POPTR	2.650e-17	55.42	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MY11_POPTR	2.650e-17	55.42	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
Q84UY4_MESCR	8.524e-16	50.59	Putative Na+/myo-inositol symporter OS=Mesembryant...	[more]
B9NEI9_POPTR	1.899e-15	53.01	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q7XUE4_ORYSJ	7.216e-15	51.90	OSJNBa0076N16.21 protein OS=Oryza sativa subsp. ja...	[more]
C5YBD4_SORBI	7.216e-15	51.90	Putative uncharacterized protein Sb06g021070 OS=So...	[more]

BLAST of FG536092 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
INT1_ARATH	1.290e-15	50.00	Probable inositol transporter 1 OS=Arabidopsis tha...	[more]
INT2_ARATH	6.417e-7	36.67	Probable inositol transporter 2 OS=Arabidopsis tha...	[more]

BLAST of FG536092 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT2G43330.1	1.424e-16	50.00	\| Symbols: ATINT1, INT1 \| inositol transporter 1 \|...	[more]
AT1G30220.1	7.083e-8	36.67	\| Symbols: ATINT2, INT2 \| inositol transporter 2 \|...	[more]
AT4G16480.1	1.208e-7	45.90	\| Symbols: ATINT4, INT4 \| inositol transporter 4 \|...	[more]
AT2G35740.1	5.996e-7	40.98	\| Symbols: ATINT3, INT3 \| nositol transporter 3 \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005828	General substrate transporter	PFAM	PF00083	Sugar_tr	coord: 2..64 score: 1.6
IPR016196	Major facilitator superfamily, general substrate transporter	SUPERFAMILY	103473	MFS general substrate transporter	coord: 1..61 score: 5.8
IPR020846	Major facilitator superfamily	PROFILE	PS50850	MFS	coord: 1..53 score: 10
None	No IPR available	PANTHER	PTHR11600	SUGAR TRANSPORTER	coord: 1..80 score: 7.8
None	No IPR available	PANTHER	PTHR11600:SF13	SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER, MEMBER 2 (GLUCOSE TRANSPORTER TYPE 2)	coord: 1..80 score: 7.8
None	No IPR available	SEG	seg	seg	coord: 32..47 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG536092 ID=FG536092; Name=FG536092; organism=Pisum sativum; type=EST; length=502bp
GGGCATGGCAGCTACCGTGTGTTGGATTTCAAACTTGATTGTGTCGGAGA
GCTTTCTTTCAATTGCTGACGGCATCGGTGTTGGTCCAACGTTCTTGATT
ATTGCTGGTATAGCTGTGGTGGCATTTTTGTTTGTATTTCTGTATGTTCC
AGAAACCCAAGGATTGACATTTGATGAAGTAGAGTTAATATGGAAGGAGA
GAGCTTGGGGCAAGAACCCAATCACACAAAGCCTTCTTGAACATGGAAGT
CAATCATAAGTATTCACATGATTAAATGTCTTGTATTCCCTCCTATCACT
ATTAATGTATTTGATCTATATAGATCAGATGCATTAGTTATAACAGTGAC
TGGAGAGAGGGTCATTTATTTAAGTATTTGCTTCATTCCATATGCACATG
CATCTTGGTTTGATATATTGTTATATTTATGTATGTGTATATGTATGTAT
TGGTATGTCAGATTTGTGTACATTAAAATTTTGATATCAAATATGACATT
AT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR005828	MFS_sugar_transport-like
IPR016196	Major facilitator superfamily domain, general substrate transporter
IPR011701	MFS

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