FG536474

EST Overview


Name	FG536474
Unique Name	FG536474
Type	EST
Organism	Pisum sativum (pea)
Sequence length	601

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG536474 vs. TrEMBL
Match: B9RP01_RICCO (Calcium ion binding protein, putative OS=Ricinus communis GN=RCOM_0922750 PE=4 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 1.887e-84
Identity = 156/200 (78.00%), Postives = 177/200 (88.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER PG L C+PS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSFSVAFKMFD++NNGEI  EEFKKVMALMR+H+RQG  HRDGLRTGLKV+ SVE+GGL+EYFFG+ GKG L  DKFVQFLRDLHDE+L+LEFAHYDYK RKTISAKDFALSMVASAD+SHL KLL R D+LN+     +++ITFE+
Sbjct:  174 SHLVREGYLSGERKPGDLRCAPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDMEEFKKVMALMRSHNRQGAVHRDGLRTGLKVSGSVEDGGLMEYFFGKYGKGRLHHDKFVQFLRDLHDEILRLEFAHYDYKLRKTISAKDFALSMVASADLSHLDKLLVRVDELNNEADLSDIQITFEE 373

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BLAST of FG536474 vs. TrEMBL
Match: B9IL14_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_911568 PE=4 SV=1)

HSP 1 Score: 309.301 bits (791), Expect = 1.352e-82
Identity = 153/200 (76.50%), Postives = 173/200 (86.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVRDGYL GER+PG L C+ S+FF LFDVNNDGLISFKEYIFF TLLSIPESSFSVAF+MFD NNNGEI KEEFKKVMALMR  +RQG  HRDGLR GLKV+ SVENGGL+E+FFG+DGK  L+ DKF+QF+RDL++E+L+LEFAHYDYK R TISAKDFALSMVASADMSHLGKLL+R D+LND      +RIT E+
Sbjct:  179 SHLVRDGYLKGERNPGELRCTSSEFFMLFDVNNDGLISFKEYIFFATLLSIPESSFSVAFRMFDFNNNGEIDKEEFKKVMALMRAQNRQGAVHRDGLRPGLKVHGSVENGGLVEHFFGKDGKASLRHDKFIQFMRDLNNEILRLEFAHYDYKLRGTISAKDFALSMVASADMSHLGKLLDRVDELNDQANLGGLRITLEE 378

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BLAST of FG536474 vs. TrEMBL
Match: D7SV13_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035935001 PE=4 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 6.281e-80
Identity = 148/200 (74.00%), Postives = 168/200 (84.00%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVRDGYL GERSPG L C PS+FF  FD+NNDGLISFKEYIF VTLLSIPE+SF VAFKMFD+NNNGEI KEEFKKVMALMR H+RQG  HRDGLR G +   SVENGGLLE+FFG+DGK  L+ +KF QF+RDLHDE+LKLEFAHYDYK R +ISAKDFALSMVASAD+S+L +LL+R D LN      ++RITFE+
Sbjct:  165 SHLVRDGYLRGERSPGELRCDPSEFFMFFDMNNDGLISFKEYIFCVTLLSIPETSFLVAFKMFDINNNGEIDKEEFKKVMALMRAHNRQGALHRDGLRFGFRTVGSVENGGLLEHFFGKDGKAKLQHEKFFQFMRDLHDELLKLEFAHYDYKLRGSISAKDFALSMVASADLSNLNRLLDRVDGLNKQSSLSDIRITFEE 364

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BLAST of FG536474 vs. TrEMBL
Match: B9SZM7_RICCO (Calcium ion binding protein, putative OS=Ricinus communis GN=RCOM_1371740 PE=4 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 6.281e-80
Identity = 145/200 (72.50%), Postives = 171/200 (85.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            S  VR+G+L GER PG L C PS+FF LFD NNDGL+SF EYIFFVTLLSIPESSFSVAFKMFD++NNGEI +EEFKKVM LMR  +RQG  HRDG R GL+V   VENGGLLEYFFG+DGK CL+ +KFVQFLRDLHDE+L+LEFAHYD+++R TISAKDFALSMVASAD+SH+ KLL+R D+LN+ P  +++RITFE+
Sbjct:  189 SDRVREGFLRGERLPGELQCEPSEFFMLFDTNNDGLVSFPEYIFFVTLLSIPESSFSVAFKMFDLDNNGEIDREEFKKVMGLMRAQNRQGATHRDGQRRGLRVLQPVENGGLLEYFFGKDGKTCLQHEKFVQFLRDLHDEILRLEFAHYDFRTRGTISAKDFALSMVASADISHINKLLDRVDELNNEPHLRDIRITFEE 388

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BLAST of FG536474 vs. TrEMBL
Match: Q9SZ45_ARATH (Putative uncharacterized protein AT4g32060 OS=Arabidopsis thaliana GN=F10N7.140 PE=2 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 5.318e-79
Identity = 144/200 (72.00%), Postives = 173/200 (86.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER+PG L CSPS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSF+VAFKMFD +NNGEI KEEFK VM+LMR+ HRQGV HRDGLRTGL +  SVE+GGL+EYFFG+DG   LK DKF +F++DL +EML+LEFAHYDYK R +ISAKDFALSMVA+AD SHL KLL+R + L+++P  ++MRI+ ++
Sbjct:  193 SHLVREGYLTGERNPGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEHPHLRDMRISLKE 392

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BLAST of FG536474 vs. TrEMBL
Match: A8MRL1_ARATH (Uncharacterized protein At4g32060.2 OS=Arabidopsis thaliana GN=At4g32060 PE=4 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 5.318e-79
Identity = 144/200 (72.00%), Postives = 173/200 (86.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER+PG L CSPS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSF+VAFKMFD +NNGEI KEEFK VM+LMR+ HRQGV HRDGLRTGL +  SVE+GGL+EYFFG+DG   LK DKF +F++DL +EML+LEFAHYDYK R +ISAKDFALSMVA+AD SHL KLL+R + L+++P  ++MRI+ ++
Sbjct:  193 SHLVREGYLTGERNPGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEHPHLRDMRISLKE 392

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BLAST of FG536474 vs. TrEMBL
Match: D7MAS4_ARALY (Calcium-binding EF hand family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_913250 PE=4 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 1.547e-78
Identity = 143/200 (71.50%), Postives = 171/200 (85.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER+PG L CSPS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSF+VAFKMFD +NNGEI KEEFK VM+LMR+ HRQGV HRDGLRTGL +  SVE+GGL+EYFFG+DG   LK DKF +F++DL +EML+LEF HYDYK R +ISAKDFALSMVA+AD SHL KLL+R + L ++P  ++MRI+ ++
Sbjct:  193 SHLVREGYLTGERNPGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFGHYDYKQRGSISAKDFALSMVAAADASHLSKLLDRVESLTEHPHLRDMRISLKE 392

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BLAST of FG536474 vs. TrEMBL
Match: Q0DZK7_ORYSJ (Os02g0613000 protein OS=Oryza sativa subsp. japonica GN=Os02g0613000 PE=4 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.310e-77
Identity = 141/200 (70.50%), Postives = 169/200 (84.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            S +VR+G L GER+PG L C+PS+FF LFD N DGLISF EYIFFVTLLSIPESSF++AFKMFD++++GEI KEEFKKVMALMR++HRQG  HRDGLR GLKV  SVENGGL+EYFFG+DG   L+ DKF  FL+ LHDE+++LEF+HYD KS KTIS KDFALSMVASADM+H+ KLL+R DD +D P  K++RITFE+
Sbjct:  170 SKIVREGSLRGERNPGELHCAPSEFFMLFDTNGDGLISFAEYIFFVTLLSIPESSFNIAFKMFDLDHSGEIDKEEFKKVMALMRSYHRQGAAHRDGLRFGLKVGQSVENGGLVEYFFGKDGNEQLRYDKFSNFLKQLHDEIVRLEFSHYDVKSSKTISVKDFALSMVASADMNHINKLLDRVDDFDDYPDLKDLRITFEE 369

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BLAST of FG536474 vs. TrEMBL
Match: B9F120_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_07508 PE=4 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.310e-77
Identity = 141/200 (70.50%), Postives = 169/200 (84.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            S +VR+G L GER+PG L C+PS+FF LFD N DGLISF EYIFFVTLLSIPESSF++AFKMFD++++GEI KEEFKKVMALMR++HRQG  HRDGLR GLKV  SVENGGL+EYFFG+DG   L+ DKF  FL+ LHDE+++LEF+HYD KS KTIS KDFALSMVASADM+H+ KLL+R DD +D P  K++RITFE+
Sbjct:  153 SKIVREGSLRGERNPGELHCAPSEFFMLFDTNGDGLISFAEYIFFVTLLSIPESSFNIAFKMFDLDHSGEIDKEEFKKVMALMRSYHRQGAAHRDGLRFGLKVGQSVENGGLVEYFFGKDGNEQLRYDKFSNFLKQLHDEIVRLEFSHYDVKSSKTISVKDFALSMVASADMNHINKLLDRVDDFDDYPDLKDLRITFEE 352

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BLAST of FG536474 vs. TrEMBL
Match: B8AFE5_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_08048 PE=4 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 1.711e-77
Identity = 140/200 (70.00%), Postives = 169/200 (84.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            S +VR+G L GER+PG L C+PS+FF LFD N DGLISF EYIFFVTLLSIPESSF++AFKMFD++++GEI KEEFKKVMALMR++HRQG  HRDGLR GLKV  SVENGGL+EYFFG+DG   L+ DKF  FL+ LHDE+++LEF+HYD KS KTIS KDFALSM+ASADM+H+ KLL+R DD +D P  K++RITFE+
Sbjct:  153 SKIVREGSLRGERNPGELHCAPSEFFMLFDTNGDGLISFAEYIFFVTLLSIPESSFNIAFKMFDLDHSGEIDKEEFKKVMALMRSYHRQGAAHRDGLRFGLKVGQSVENGGLVEYFFGKDGNEQLRYDKFSNFLKQLHDEIVRLEFSHYDVKSSKTISVKDFALSMIASADMNHINKLLDRVDDFDDYPDLKDLRITFEE 352

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_DANRE (Calcium-binding atopy-related autoantigen 1 OS=Danio rerio GN=cbara1 PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.958e-24
Identity = 72/184 (39.13%), Postives = 104/184 (56.52%), Query Frame = 2

Query:   68 SDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDF-----ALSMVASADMSHLGKLLERADDLNDNPCFKEMR-ITFED 601
            S F+TL +    GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +     +  L  YFFG D KG L +  F++F R L  ++LKLEF   D    + I+ K F     A S V S  +  + K L+R         FK+ + ITFE+
Sbjct:  204 SIFYTLGEC---GLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDLEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKTGGCSSALTTYFFGADLKGKLTISSFLEFQRKLQHDVLKLEFERNDPVDGR-ITEKQFGGMLLAYSGVQSRKLKQMQKNLKR--------MFKDAQGITFEE 375

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_MOUSE (Calcium-binding atopy-related autoantigen 1 OS=Mus musculus GN=Cbara1 PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.272e-23
Identity = 60/154 (38.96%), Postives = 88/154 (57.14%), Query Frame = 2

Query:    5 HLVRDGYLIGERSPGHLCCSPSDFFT-----LFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYD 451
            HL  D Y+I +R  G       + F       + +   GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +   +    L  YFFG D KG L +  F++F R L  ++LKLEF  +D
Sbjct:  167 HLGLDQYII-KRFDGKKIAQEREKFADEGSIFYSLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGL-CSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD 318

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_RAT (Calcium-binding atopy-related autoantigen 1 OS=Rattus norvegicus GN=Cbara1 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.579e-23
Identity = 60/154 (38.96%), Postives = 88/154 (57.14%), Query Frame = 2

Query:    5 HLVRDGYLIGERSPGHLCCSPSDFFT-----LFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYD 451
            HL  D Y+I +R  G       + F       + +   GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +   +    L  YFFG D KG L +  F++F R L  ++LKLEF  +D
Sbjct:  167 HLGLDQYII-KRFDGKKIAQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGL-CSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD 318

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_BOVIN (Calcium-binding atopy-related autoantigen 1 OS=Bos taurus GN=CBARA1 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.579e-23
Identity = 56/128 (43.75%), Postives = 81/128 (63.28%), Query Frame = 2

Query:   68 SDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYD 451
            S F+TL +    GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +   +    L  YFFG D KG L +  F++F R L  ++LKLEF  +D
Sbjct:  195 SIFYTLGEC---GLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKSGL-CSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD 318

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_MACFA (Calcium-binding atopy-related autoantigen 1 OS=Macaca fascicularis GN=CBARA1 PE=2 SV=2)

HSP 1 Score: 107.071 bits (266), Expect = 7.287e-23
Identity = 56/128 (43.75%), Postives = 81/128 (63.28%), Query Frame = 2

Query:   68 SDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYD 451
            S F+TL +    GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +   +    L  YFFG D KG L +  F++F R L  ++LKLEF  +D
Sbjct:  193 SIFYTLGEC---GLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGL-CSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD 316

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BLAST of FG536474 vs. SwissProt
Match: CBAA1_HUMAN (Calcium-binding atopy-related autoantigen 1 OS=Homo sapiens GN=CBARA1 PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.287e-23
Identity = 56/128 (43.75%), Postives = 81/128 (63.28%), Query Frame = 2

Query:   68 SDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYD 451
            S F+TL +    GLISF +YIF  T+LS P+ +F +AFKMFD+N +GE+  EEF++V +++R+    G+ HRD   TG  +   +    L  YFFG D KG L +  F++F R L  ++LKLEF  +D
Sbjct:  193 SIFYTLGEC---GLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGL-CSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD 316

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BLAST of FG536474 vs. SwissProt
Match: CMC_DICDI (Calcium-binding mitochondrial carrier protein OS=Dictyostelium discoideum GN=mcfO PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.836e-13
Identity = 53/165 (32.12%), Postives = 81/165 (49.09%), Query Frame = 2

Query:   77 FTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADM----SHLGKLLERADDLN 559
            F + DV++ G ISF EY+ F  L++ PE+ + +AFK+FD + NG I+K +FK V+                L   +  N   E   L+  +FG DG+  L   +F Q L+DL  E +K EF  +D  +   I    FA  ++ S  +     H+   LE   +LN
Sbjct:  141 FQMADVDHTGYISFDEYVMFDELMAKPEAEYFLAFKLFDRDGNGYISKNDFKHVITA-------------SLDPSIPFNFDCE---LVNLYFG-DGRTELNYSQFTQLLKDLQQERIKQEFKFHDKYNSGYIPRDKFA-KVLGSVKLRKIPDHVRDKLESISELN 287

[Close]

BLAST of FG536474 vs. SwissProt
Match: EFHA1_MOUSE (EF-hand domain-containing family member A1 OS=Mus musculus GN=Efha1 PE=2 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 5.787e-12
Identity = 49/151 (32.45%), Postives = 80/151 (52.98%), Query Frame = 2

Query:   68 SDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSV----------ENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFA 490
            S FF   D+ + G+IS+ EY+F +T+L+ P S F VAFKM DV+ N  I ++EF K+  +        +  +DG +T +K N++            N  L   FFG+ G+  L   +F +F+ +L  E+ ++EF  +  K    +  +DFA
Sbjct:  143 STFFR--DLGDKGVISYTEYLFLLTILTKPHSGFHVAFKMLDVDGNEMIERKEFVKLQKI--------ISKQDGFKT-VKTNETEYQDPTVKEPGVNTTLQVRFFGKRGEKKLHYKEFRRFMENLQTEVQEMEFLQFS-KGLNFMRKEDFA 281

[Close]

BLAST of FG536474 vs. SwissProt
Match: CMC1_PONAB (Calcium-binding mitochondrial carrier protein Aralar1 OS=Pongo abelii GN=SLC25A12 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.787e-12
Identity = 46/138 (33.33%), Postives = 67/138 (48.55%), Query Frame = 2

Query:   89 DVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMV 502
            D   DGLIS++E++ F ++L  P+S F VAF++FD + NGE+T E  K++      HH             +  N   E    +   FG + K  L   +F QFL++L  E  +  FA  D      IS  DF+  MV
Sbjct:   65 DQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHH------------IPFNWDCE---FIRLHFGHNRKKHLNYTEFTQFLQELQLEHARQAFALKDKSKSGVISGLDFSDIMV 187

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BLAST of FG536474 vs. SwissProt
Match: CMC1_HUMAN (Calcium-binding mitochondrial carrier protein Aralar1 OS=Homo sapiens GN=SLC25A12 PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 7.558e-12
Identity = 46/138 (33.33%), Postives = 67/138 (48.55%), Query Frame = 2

Query:   89 DVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMV 502
            D   DGLIS++E++ F ++L  P+S F VAF++FD + NGE+T E  K++      HH             +  N   E    +   FG + K  L   +F QFL++L  E  +  FA  D      IS  DF+  MV
Sbjct:   65 DQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHH------------IPFNWDCE---FIRLHFGHNRKKHLNYTEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDIMV 187

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BLAST of FG536474 vs. TAIR peptide
Match: AT4G32060.2 (| Symbols: | calcium-binding EF hand family protein | chr4:15501134-15503302 FORWARD LENGTH=454)

HSP 1 Score: 297.36 bits (760), Expect = 3.970e-81
Identity = 144/200 (72.00%), Postives = 173/200 (86.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER+PG L CSPS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSF+VAFKMFD +NNGEI KEEFK VM+LMR+ HRQGV HRDGLRTGL +  SVE+GGL+EYFFG+DG   LK DKF +F++DL +EML+LEFAHYDYK R +ISAKDFALSMVA+AD SHL KLL+R + L+++P  ++MRI+ ++
Sbjct:  193 SHLVREGYLTGERNPGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEHPHLRDMRISLKE 392

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BLAST of FG536474 vs. TAIR peptide
Match: AT4G32060.1 (| Symbols: | calcium-binding EF hand family protein | chr4:15501134-15503321 FORWARD LENGTH=498)

HSP 1 Score: 297.36 bits (760), Expect = 3.970e-81
Identity = 144/200 (72.00%), Postives = 173/200 (86.50%), Query Frame = 2

Query:    2 SHLVRDGYLIGERSPGHLCCSPSDFFTLFDVNNDGLISFKEYIFFVTLLSIPESSFSVAFKMFDVNNNGEITKEEFKKVMALMRTHHRQGVHHRDGLRTGLKVNDSVENGGLLEYFFGEDGKGCLKLDKFVQFLRDLHDEMLKLEFAHYDYKSRKTISAKDFALSMVASADMSHLGKLLERADDLNDNPCFKEMRITFED 601
            SHLVR+GYL GER+PG L CSPS+FF LFDV+NDGLISFKEYIFFVTLLSIPESSF+VAFKMFD +NNGEI KEEFK VM+LMR+ HRQGV HRDGLRTGL +  SVE+GGL+EYFFG+DG   LK DKF +F++DL +EML+LEFAHYDYK R +ISAKDFALSMVA+AD SHL KLL+R + L+++P  ++MRI+ ++
Sbjct:  193 SHLVREGYLTGERNPGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLDRVESLSEHPHLRDMRISLKE 392

The following BLAST results are available for this feature:

BLAST of FG536474 vs. TrEMBL
BLAST of FG536474 vs. SwissProt
BLAST of FG536474 vs. TAIR peptide

BLAST of FG536474 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RP01_RICCO	1.887e-84	78.00	Calcium ion binding protein, putative OS=Ricinus c...	[more]
B9IL14_POPTR	1.352e-82	76.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SV13_VITVI	6.281e-80	74.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SZM7_RICCO	6.281e-80	72.50	Calcium ion binding protein, putative OS=Ricinus c...	[more]
Q9SZ45_ARATH	5.318e-79	72.00	Putative uncharacterized protein AT4g32060 OS=Arab...	[more]
A8MRL1_ARATH	5.318e-79	72.00	Uncharacterized protein At4g32060.2 OS=Arabidopsis...	[more]
D7MAS4_ARALY	1.547e-78	71.50	Calcium-binding EF hand family protein OS=Arabidop...	[more]
Q0DZK7_ORYSJ	1.310e-77	70.50	Os02g0613000 protein OS=Oryza sativa subsp. japoni...	[more]
B9F120_ORYSJ	1.310e-77	70.50	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AFE5_ORYSI	1.711e-77	70.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG536474 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CBAA1_DANRE	2.958e-24	39.13	Calcium-binding atopy-related autoantigen 1 OS=Dan...	[more]
CBAA1_MOUSE	4.272e-23	38.96	Calcium-binding atopy-related autoantigen 1 OS=Mus...	[more]
CBAA1_RAT	5.579e-23	38.96	Calcium-binding atopy-related autoantigen 1 OS=Rat...	[more]
CBAA1_BOVIN	5.579e-23	43.75	Calcium-binding atopy-related autoantigen 1 OS=Bos...	[more]
CBAA1_MACFA	7.287e-23	43.75	Calcium-binding atopy-related autoantigen 1 OS=Mac...	[more]
CBAA1_HUMAN	7.287e-23	43.75	Calcium-binding atopy-related autoantigen 1 OS=Hom...	[more]
CMC_DICDI	6.836e-13	32.12	Calcium-binding mitochondrial carrier protein OS=D...	[more]
EFHA1_MOUSE	5.787e-12	32.45	EF-hand domain-containing family member A1 OS=Mus ...	[more]
CMC1_PONAB	5.787e-12	33.33	Calcium-binding mitochondrial carrier protein Aral...	[more]
CMC1_HUMAN	7.558e-12	33.33	Calcium-binding mitochondrial carrier protein Aral...	[more]

BLAST of FG536474 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT4G32060.2	3.970e-81	72.00	\| Symbols: \| calcium-binding EF hand family prote...	[more]
AT4G32060.1	3.970e-81	72.00	\| Symbols: \| calcium-binding EF hand family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	Calcium-binding EF-hand	SMART	SM00054	no description	coord: 21..49 score: 0.14coord: 55..83 score: 7.2
IPR011992	EF-hand-like domain	GENE3D	1.10.238.10	no description	coord: 133..163 score: 3.9e-17coord: 69..132 score: 3.7
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76 score: NAcoord: 30..42 score
IPR018248	EF-hand	PFAM	PF00036	efhand	coord: 59..82 score: 4.5
IPR018249	EF-HAND 2	PROFILE	PS50222	EF_HAND_2	coord: 51..86 score: 13.398coord: 24..49 score: 6.396coord: 120..140 score: 5
None	No IPR available	PANTHER	PTHR12294	EF HAND DOMAIN FAMILY A1,A2-RELATED	coord: 2..200 score: 3.2
None	No IPR available	PANTHER	PTHR12294:SF1	CALCIUM BINDING ATOPY-RELATED AUTOANTIGEN 1	coord: 2..200 score: 3.2
None	No IPR available	SUPERFAMILY	47473	EF-hand	coord: 25..160 score: 4.4
None	No IPR available	SEG	seg	seg	coord: 3..12 score: NAcoord: 25..40 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG536474 ID=FG536474; Name=FG536474; organism=Pisum sativum; type=EST; length=601bp
ATCTCACCTTGTTAGAGATGGATACTTGATAGGAGAAAGAAGCCCTGGCC
ATTTATGCTGCTCTCCTTCAGATTTTTTTACGCTTTTCGATGTAAACAAT
GACGGACTTATATCATTTAAAGAGTATATCTTTTTTGTAACGCTACTCAG
CATCCCAGAATCAAGCTTTTCCGTGGCATTTAAAATGTTTGATGTGAACA
ACAATGGAGAGATAACCAAGGAAGAATTCAAGAAAGTGATGGCATTGATG
CGAACTCATCATCGACAAGGTGTTCACCACAGGGATGGACTGCGAACTGG
TCTGAAGGTGAACGATTCTGTGGAAAATGGAGGGCTGTTGGAATACTTTT
TTGGTGAAGACGGAAAGGGTTGCCTCAAACTTGATAAATTTGTGCAATTT
TTACGAGACTTGCATGATGAAATGCTGAAGCTGGAGTTTGCTCACTATGA
CTACAAATCTCGAAAAACAATTTCAGCCAAGGACTTTGCACTCTCCATGG
TTGCTTCCGCCGACATGAGTCATCTAGGCAAGTTGCTTGAACGGGCTGAT
GATTTGAATGATAATCCATGCTTTAAGGAGATGCGTATCACATTTGAGGA
C

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR018248	EF-hand
IPR018249	EF-HAND 2

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