GH720347

EST Overview
NameGH720347
Unique NameGH720347
TypeEST
OrganismPisum sativum (pea)
Sequence length700
Libraries
Library NameType
LIBEST_024270 552Icdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of GH720347 vs. TrEMBL
Match: B9RTF7_RICCO (Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, putative OS=Ricinus communis GN=RCOM_0909300 PE=4 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 5.915e-90
Identity = 155/209 (74.16%), Postives = 168/209 (80.38%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAFLSVWR+YGFTPEGFNLA+LSVQHGQKSYPLRPEL+ESTYWLYKATRD RYLDAGRDM+ASLQYG RCPCGYCHI+DVE HK+EDHMESFFLAETVKYLWLLFDLA GPDNLVENGPYKY+FSTEGHLLPA PQISL+ EHC Y+GAYC S D++Q    S    + HE N S FYG                   SGL+KG CP
Sbjct:  339 THAAFLSVWRKYGFTPEGFNLATLSVQHGQKSYPLRPELIESTYWLYKATRDSRYLDAGRDMVASLQYGARCPCGYCHITDVEFHKKEDHMESFFLAETVKYLWLLFDLASGPDNLVENGPYKYIFSTEGHLLPAMPQISLLGEHCSYFGAYCESDDIKQESPKSSISVNSHEINASTFYGGQVHPRYPLDSTFFESTATSGLMKGICP 547

HSP 2 Score: 28.1054 bits (61), Expect = 5.915e-90
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL HGQK+G+ YL
Sbjct:  547 PGLTHGQKYGISYL 560
[Close]
BLAST of GH720347 vs. TrEMBL
Match: D7T783_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018097001 PE=4 SV=1)

HSP 1 Score: 319.701 bits (818), Expect = 1.205e-87
Identity = 151/209 (72.25%), Postives = 164/209 (78.47%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            TH AF SVW+RYGFTPEGFNLA+LSVQHGQKSYPLRPEL+ESTYWLYKATR+PRYLDAGRDM+ SLQYG RCPCGYCHISDVE HKQEDHMESFFLAETVKYLWLLFDLA G DNLVENGPYKY+FSTEGHLLPATPQISL+REHC Y+GAYC + D RQ     +   D H+ N S+ Y                    SG +KG CP
Sbjct:  370 THTAFFSVWKRYGFTPEGFNLATLSVQHGQKSYPLRPELMESTYWLYKATRNPRYLDAGRDMVTSLQYGARCPCGYCHISDVEFHKQEDHMESFFLAETVKYLWLLFDLAAGSDNLVENGPYKYIFSTEGHLLPATPQISLIREHCSYFGAYCNNDDSRQDSRTLDLSTD-HQENKSQLYRGWDPSSSPSDNTLREPTSISGSMKGLCP 577

HSP 2 Score: 29.261 bits (64), Expect = 1.205e-87
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL HGQKFG+ YL
Sbjct:  577 PGLTHGQKFGISYL 590
[Close]
BLAST of GH720347 vs. TrEMBL
Match: B9HKD6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_419089 PE=4 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 1.720e-86
Identity = 149/209 (71.29%), Postives = 162/209 (77.51%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAF SVW+RYGFTPEGFNLA+L+VQHGQKSYPLRPEL+ESTYWLYKATRDPRYLD GRDM+ SLQYG RCPCGYCHI DVE HK+EDHMESFFLAETVKYLWLLFDLA GPDNLVENGPYKY+FSTEGHLLPATPQISL+ EHC Y GAYC+ G + Q    S+      E+N S   G                   SGL+KG CP
Sbjct:  311 THAAFFSVWKRYGFTPEGFNLATLTVQHGQKSYPLRPELIESTYWLYKATRDPRYLDVGRDMVVSLQYGARCPCGYCHIVDVEFHKKEDHMESFFLAETVKYLWLLFDLASGPDNLVENGPYKYIFSTEGHLLPATPQISLIHEHCSYLGAYCKGGGIEQESRKSDISIHPQENNASVLSGSWVHSSYPSDSSFLESTSTSGLMKGLCP 519

HSP 2 Score: 28.1054 bits (61), Expect = 1.720e-86
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL HGQK+G+ YL
Sbjct:  519 PGLTHGQKYGISYL 532
[Close]
BLAST of GH720347 vs. TrEMBL
Match: Q9FG93_ARATH (Dbj|BAA91806.1 OS=Arabidopsis thaliana GN=At5g43710 PE=2 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 2.058e-81
Identity = 147/211 (69.67%), Postives = 162/211 (76.78%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGD--LRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            TH AF SVW+RYGFTPEGFNLA+LSVQ+GQKSYPLRPEL+ESTYWLYKATRDPRYLDAGRD +ASLQYG +CPCGYCHI+DVE HKQEDHMESFFLAETVKYLWLLFDLAV  DNLV+NGPYKY+FSTEGHLLP TPQISL REHC Y+G YC S    L Q     ++  D H SND  ++                    +GLIKG CP
Sbjct:  339 THTAFFSVWKRYGFTPEGFNLATLSVQYGQKSYPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHKQEDHMESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPITPQISLAREHCSYFGGYCPSNSTKLEQEVLGEDSSNDDH-SNDYPYH---------------ESFPVTGLIKGLCP 533

HSP 2 Score: 23.8682 bits (50), Expect = 2.058e-81
Identity = 8/14 (57.14%), Postives = 10/14 (71.43%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL H QK+G  Y+
Sbjct:  533 PGLTHAQKYGFSYV 546
[Close]
BLAST of GH720347 vs. TrEMBL
Match: D7MNM7_ARALY (Glycoside hydrolase family 47 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_494638 PE=4 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 2.934e-80
Identity = 145/209 (69.38%), Postives = 158/209 (75.60%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            TH AF SVW+RYGFTPEGFNLA+LSVQ+GQKSYPLRPEL+ESTYWLYKATRDPRYLDAGRD +ASLQYG +CPCGYCHI+DVE HKQEDHMESFFLAETVKYLWLLFDLAV  DNLV+NGPYKY+FSTEGHLLP TPQISL RE C Y+G YC S   +    V E E     +ND R                      +GLIKG CP
Sbjct:  339 THTAFFSVWKRYGFTPEGFNLATLSVQYGQKSYPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHKQEDHMESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPLTPQISLARERCSYFGGYCPSNSTKLEQEVLEEE----SNNDDR----------SNIYPYHESFPITGLIKGLCP 533

HSP 2 Score: 25.409 bits (54), Expect = 2.934e-80
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL H QK+GL Y+
Sbjct:  533 PGLTHAQKYGLSYV 546
[Close]
BLAST of GH720347 vs. TrEMBL
Match: Q0JIW3_ORYSJ (Os01g0773600 protein OS=Oryza sativa subsp. japonica GN=Os01g0773600 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 8.484e-77
Identity = 137/209 (65.55%), Postives = 158/209 (75.60%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAF SVW++YGFTPEGFNLA+ +VQ+GQ+SYPLRPEL+ESTYWL+KATRD RYLD GRD+LASLQYG +CPCGYCHISDVE H+Q+DHMESFFLAETVKYLWLLFDLA GPDN+VENGPYKY+FSTEGHLLPATPQI+L+ EHCLY GA+C +G     Y   ++      +N   F                      G IKG CP
Sbjct:  355 THAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDYRYLDVGRDILASLQYGAKCPCGYCHISDVETHQQDDHMESFFLAETVKYLWLLFDLAAGPDNIVENGPYKYIFSTEGHLLPATPQITLLDEHCLYAGAFC-NGTAGHGYGTGDSSNKHQNANSVPF---DDSRTPSDQSLSNILSTTRGYIKGVCP 559

HSP 2 Score: 23.0978 bits (48), Expect = 8.484e-77
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = -1 
Query:   20 PGLNHGQKFGLLY 58
            PGL H QK G+ Y
Sbjct:  559 PGLTHAQKLGISY 571
[Close]
BLAST of GH720347 vs. TrEMBL
Match: Q5ZBZ0_ORYSJ (Putative aspartyl(D) tRNA Synthetase (89.0 kD)(Drs-2C) OS=Oryza sativa subsp. japonica GN=P0490D09.16 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 8.485e-77
Identity = 137/209 (65.55%), Postives = 158/209 (75.60%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAF SVW++YGFTPEGFNLA+ +VQ+GQ+SYPLRPEL+ESTYWL+KATRD RYLD GRD+LASLQYG +CPCGYCHISDVE H+Q+DHMESFFLAETVKYLWLLFDLA GPDN+VENGPYKY+FSTEGHLLPATPQI+L+ EHCLY GA+C +G     Y   ++      +N   F                      G IKG CP
Sbjct:  347 THAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDYRYLDVGRDILASLQYGAKCPCGYCHISDVETHQQDDHMESFFLAETVKYLWLLFDLAAGPDNIVENGPYKYIFSTEGHLLPATPQITLLDEHCLYAGAFC-NGTAGHGYGTGDSSNKHQNANSVPF---DDSRTPSDQSLSNILSTTRGYIKGVCP 551

HSP 2 Score: 23.0978 bits (48), Expect = 8.485e-77
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = -1 
Query:   20 PGLNHGQKFGLLY 58
            PGL H QK G+ Y
Sbjct:  551 PGLTHAQKLGISY 563
[Close]
BLAST of GH720347 vs. TrEMBL
Match: B9ET98_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_03634 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 8.487e-77
Identity = 137/209 (65.55%), Postives = 158/209 (75.60%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAF SVW++YGFTPEGFNLA+ +VQ+GQ+SYPLRPEL+ESTYWL+KATRD RYLD GRD+LASLQYG +CPCGYCHISDVE H+Q+DHMESFFLAETVKYLWLLFDLA GPDN+VENGPYKY+FSTEGHLLPATPQI+L+ EHCLY GA+C +G     Y   ++      +N   F                      G IKG CP
Sbjct:  324 THAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDYRYLDVGRDILASLQYGAKCPCGYCHISDVETHQQDDHMESFFLAETVKYLWLLFDLAAGPDNIVENGPYKYIFSTEGHLLPATPQITLLDEHCLYAGAFC-NGTAGHGYGTGDSSNKHQNANSVPF---DDSRTPSDQSLSNILSTTRGYIKGVCP 528

HSP 2 Score: 23.0978 bits (48), Expect = 8.487e-77
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = -1 
Query:   20 PGLNHGQKFGLLY 58
            PGL H QK G+ Y
Sbjct:  528 PGLTHAQKLGISY 540
[Close]
BLAST of GH720347 vs. TrEMBL
Match: B8AA67_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_03924 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 8.488e-77
Identity = 137/209 (65.55%), Postives = 158/209 (75.60%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            THAAF SVW++YGFTPEGFNLA+ +VQ+GQ+SYPLRPEL+ESTYWL+KATRD RYLD GRD+LASLQYG +CPCGYCHISDVE H+Q+DHMESFFLAETVKYLWLLFDLA GPDN+VENGPYKY+FSTEGHLLPATPQI+L+ EHCLY GA+C +G     Y   ++      +N   F                      G IKG CP
Sbjct:  324 THAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDYRYLDVGRDILASLQYGAKCPCGYCHISDVETHQQDDHMESFFLAETVKYLWLLFDLAAGPDNIVENGPYKYIFSTEGHLLPATPQITLLDEHCLYAGAFC-NGTAGHGYGTGDSSNKHQNANSVPF---DDSRTPSDQSLSNILSTTRGYIKGVCP 528

HSP 2 Score: 23.0978 bits (48), Expect = 8.488e-77
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = -1 
Query:   20 PGLNHGQKFGLLY 58
            PGL H QK G+ Y
Sbjct:  528 PGLTHAQKLGISY 540
[Close]
BLAST of GH720347 vs. TrEMBL
Match: C5XLL0_SORBI (Putative uncharacterized protein Sb03g036080 OS=Sorghum bicolor GN=Sb03g036080 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 3.439e-76
Identity = 129/176 (73.30%), Postives = 152/176 (86.36%), Query Frame = -3 
Query:  156 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGDLRQTYFVSEAERDKHESND 683
            THAAF SVW++YGFTPEGFNLA+ +VQ+GQ+SYPLRPEL+ESTYWL+KATRD RYLD GRD+LASLQYG RCPCGYCHI+DVE HKQ+DHMESFFLAETVKYLWLLFDLA GPDN+VE+GPYKY+FSTEGHLLP TP+I+LV EHC Y+GA+C  G      + + A   KH++ +
Sbjct:  343 THAAFFSVWKKYGFTPEGFNLATSTVQNGQRSYPLRPELIESTYWLFKATRDHRYLDVGRDILASLQYGARCPCGYCHIADVETHKQDDHMESFFLAETVKYLWLLFDLAAGPDNIVESGPYKYIFSTEGHLLPVTPEIALVDEHCSYFGAFCNGG--ADHGYGASASSTKHQNGN 516
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM1_HUMAN (ER degradation-enhancing alpha-mannosidase-like 1 OS=Homo sapiens GN=EDEM1 PE=1 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.222e-27
Identity = 66/134 (49.25%), Postives = 86/134 (64.18%), Query Frame = -3 
Query:  285 HAAFLSVWRRYGFTPEGFN--LASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLL 680
            HA + ++W+RYG  PE +N  L +  V      YPLRPELVESTY LY+AT++P YL  G D+L SL+  T+  CGY  +  V     ED MESFFL+ET KYL+LLFD     DN V     +Y+F+TEGH++
Sbjct:  459 HAFYYAIWKRYGALPERYNWQLQAPDVLF----YPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFD----EDNPVHKSGTRYMFTTEGHIV 584
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM1_MOUSE (ER degradation-enhancing alpha-mannosidase-like 1 OS=Mus musculus GN=Edem1 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.903e-27
Identity = 65/134 (48.51%), Postives = 87/134 (64.93%), Query Frame = -3 
Query:  285 HAAFLSVWRRYGFTPEGFN--LASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLL 680
            HA + ++W+RYG  PE +N  L +  V      YPLRPELVESTY LY+AT++P YL  G D+L SL+  T+  CGY  +  V    +ED MESFFL+ET KYL+LLFD     +N V     +Y+F+TEGH++
Sbjct:  454 HAFYYAIWKRYGALPERYNWQLQAPDVLF----YPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFD----EENPVHKSGTRYMFTTEGHII 579
[Close]
BLAST of GH720347 vs. SwissProt
Match: MNL1_SCHPO (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Schizosaccharomyces pombe GN=mnl1 PE=2 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 3.791e-27
Identity = 60/149 (40.27%), Postives = 92/149 (61.74%), Query Frame = -3 
Query:  234 HAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYG 680
            H  + S++ ++G  PE +NL + S++     YPLRPE  ESTY+LY+AT+D  YL  G  +L++++     PCG+  + ++E +   + MESFFL+ET+KYL+LLFD     DN +    + ++F+TEGHL P T Q  L     +Y G
Sbjct:  350 HLYYFSIYLKFGQLPERYNLYTKSIE--LNGYPLRPEFAESTYYLYRATKDVFYLHVGELLLSNIENHLWTPCGFAAVENLEKYTLSNRMESFFLSETLKYLFLLFD----DDNPIHRSHHDFIFTTEGHLFPVTNQTRLSTSQRIYDG 492
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM2_HUMAN (ER degradation-enhancing alpha-mannosidase-like 2 OS=Homo sapiens GN=EDEM2 PE=1 SV=2)

HSP 1 Score: 114.39 bits (285), Expect = 6.052e-25
Identity = 60/148 (40.54%), Postives = 86/148 (58.11%), Query Frame = -3 
Query:  291 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENG---------PY--------KYVFSTEGH 683
            T   + +VW+++G  PE +N+        ++ YPLRPEL+ES  +LY+AT DP  L+ GRD + S++  ++  CG+  I D+  HK ++ MESFFLAETVKYL+LLFD    P N + N          PY         Y+F+TE H
Sbjct:  335 TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFD----PTNFIHNNGSTFDAVITPYGECILGAGGYIFNTEAH 478
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM3_XENLA (ER degradation-enhancing alpha-mannosidase-like 3 OS=Xenopus laevis GN=edem3 PE=2 SV=2)

HSP 1 Score: 107.071 bits (266), Expect = 9.663e-23
Identity = 59/134 (44.03%), Postives = 77/134 (57.46%), Query Frame = -3 
Query:  282 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 683
            TH     V +++ F PE F      V   Q  +PLRPE  ESTY+LYKAT DP YL+ G+ ++ +L    R PCG+  + DV     ED M+SFFLAE  KYL+LLF         +E+    Y+F+TE HLLP
Sbjct:  357 THEMLYQVIKKHNFLPEAFT-TDFRVHWAQ--HPLRPEFAESTYFLYKATGDPYYLEVGKTLIDNLNKYARVPCGFAAVKDVRTGSHEDRMDSFFLAEMFKYLYLLFSEREDLIFDIED----YIFTTEAHLLP 483
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM3_MOUSE (ER degradation-enhancing alpha-mannosidase-like 3 OS=Mus musculus GN=Edem3 PE=1 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 2.153e-22
Identity = 60/134 (44.78%), Postives = 78/134 (58.21%), Query Frame = -3 
Query:  282 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 683
            TH     V +++ F PE F      V   Q  +PLRPE  ESTY+LYKAT DP YL+ G+ ++ +L    R PCG+  + DV     ED M+SFFLAE  KYL+LLF  A   D + +     Y+F+TE HLLP
Sbjct:  372 THEMLYQVIKKHNFLPEAFT-TDFRVHWAQ--HPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLF--ADKEDIIFD--IEDYIFTTEAHLLP 498
[Close]
BLAST of GH720347 vs. SwissProt
Match: EDEM3_HUMAN (ER degradation-enhancing alpha-mannosidase-like 3 OS=Homo sapiens GN=EDEM3 PE=1 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 2.153e-22
Identity = 60/134 (44.78%), Postives = 78/134 (58.21%), Query Frame = -3 
Query:  282 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 683
            TH     V +++ F PE F      V   Q  +PLRPE  ESTY+LYKAT DP YL+ G+ ++ +L    R PCG+  + DV     ED M+SFFLAE  KYL+LLF  A   D + +     Y+F+TE HLLP
Sbjct:  371 THEMLYQVIKKHNFLPEAFT-TDFRVHWAQ--HPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLF--ADKEDIIFD--IEDYIFTTEAHLLP 497
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BLAST of GH720347 vs. SwissProt
Match: MNL1_YEAST (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Saccharomyces cerevisiae GN=MNL1 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 6.481e-19
Identity = 54/146 (36.99%), Postives = 78/146 (53.42%), Query Frame = -3 
Query:  291 HAAFLSVWRRYGFTPEGFNLA----------------SLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGH 680
            H  FL +W  +G  PE +N +                +L      + YPLRPE  ESTY+LY+AT+DP YL+ G  +L  L+   +  CG+    +V   + +D ME+F L+ET+KYL+LLFD     +N + N     +FSTE H
Sbjct:  357 HLMFLKMWNTFGGIPERWNFSPPEFPPLSPLERSGAVALDNILPLEWYPLRPEFFESTYFLYRATKDPFYLNIGVHLLKDLKQRFKSNCGFAGFQNVITGELQDRMETFVLSETLKYLYLLFD----EENELHNSASDVIFSTEAH 498
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BLAST of GH720347 vs. SwissProt
Match: MA1B1_RAT (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus norvegicus GN=Man1b1 PE=2 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 2.158e-14
Identity = 50/104 (48.08%), Postives = 66/104 (63.46%), Query Frame = -3 
Query:  282 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPC-GYCHISDVEH-HKQE--DHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 581
            LRPE VES ++LY+ T+D +Y D G ++L S    TR P  GY  I++V++ HK E  D MESFF+ ET+KYL+LLF      D+L   G    VF+TE H LP
Sbjct:  554 LRPETVESLFYLYRVTKDRKYQDWGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFS-----DDLELLGLDTCVFNTEAHPLP 652
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BLAST of GH720347 vs. SwissProt
Match: MA1B1_MOUSE (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musculus GN=Man1b1 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.680e-14
Identity = 50/104 (48.08%), Postives = 65/104 (62.50%), Query Frame = -3 
Query:  282 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPC-GYCHISDVEH-HKQE--DHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 581
            LRPE VES ++LY+ TRD +Y D G ++L S    TR P  GY  I++V++ HK E  D MESFF+ ET+KYL+LLF      D+L        VF+TE H LP
Sbjct:  555 LRPETVESLFYLYRVTRDRKYQDWGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFS-----DDLELLSLDSCVFNTEAHPLP 653
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BLAST of GH720347 vs. TAIR peptide
Match: AT5G43710.1 (| Symbols: | Glycosyl hydrolase family 47 protein | chr5:17552252-17556523 REVERSE LENGTH=624)

HSP 1 Score: 304.294 bits (778), Expect = 1.408e-83
Identity = 147/211 (69.67%), Postives = 162/211 (76.78%), Query Frame = -3 
Query:   57 THAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLPATPQISLVREHCLYYGAYCRSGD--LRQTYFVSEAERDKHESNDSRFYGXXXXXXXXXXXXXXXXXXXSGLIKGFCP 683
            TH AF SVW+RYGFTPEGFNLA+LSVQ+GQKSYPLRPEL+ESTYWLYKATRDPRYLDAGRD +ASLQYG +CPCGYCHI+DVE HKQEDHMESFFLAETVKYLWLLFDLAV  DNLV+NGPYKY+FSTEGHLLP TPQISL REHC Y+G YC S    L Q     ++  D H SND  ++                    +GLIKG CP
Sbjct:  339 THTAFFSVWKRYGFTPEGFNLATLSVQYGQKSYPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHKQEDHMESFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPITPQISLAREHCSYFGGYCPSNSTKLEQEVLGEDSSNDDH-SNDYPYH---------------ESFPVTGLIKGLCP 533

HSP 2 Score: 23.8682 bits (50), Expect = 1.408e-83
Identity = 8/14 (57.14%), Postives = 10/14 (71.43%), Query Frame = -1 
Query:   17 PGLNHGQKFGLLYL 58
            PGL H QK+G  Y+
Sbjct:  533 PGLTHAQKYGFSYV 546
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BLAST of GH720347 vs. TAIR peptide
Match: AT1G27520.1 (| Symbols: | Glycosyl hydrolase family 47 protein | chr1:9558752-9562091 FORWARD LENGTH=574)

HSP 1 Score: 122.865 bits (307), Expect = 1.712e-28
Identity = 64/136 (47.06%), Postives = 82/136 (60.29%), Query Frame = -3 
Query:  291 AARAGTHAAFLSVWRRYGFTPEGFNLASLSVQHGQKSYPLRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGH 698
            AA   +H  F  VW ++G  PE + L    +    K YPLRPEL EST++LY+AT+DP YLD G  M+ SL   T+ P G+  + DV   + EDH  SFFLAET KYL+LLFD     D+ V      Y+F+TEGH
Sbjct:  346 AAANSSHREFFHVWEKFGVLPERYLLDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKVPGGFASVRDVTTMQLEDHQHSFFLAETCKYLYLLFD-----DSFV--AKRNYIFTTEGH 474
[Close]
BLAST of GH720347 vs. TAIR peptide
Match: AT1G30000.1 (| Symbols: MNS3 | alpha-mannosidase 3 | chr1:10509532-10512320 REVERSE LENGTH=624)

HSP 1 Score: 70.4774 bits (171), Expect = 1.008e-12
Identity = 43/103 (41.75%), Postives = 61/103 (59.22%), Query Frame = -3 
Query:  282 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPC-GYCHISDVEH--HKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLLP 581
            LRPE VES + LY+ T+D +Y D G  +  + +  T+    GY  + DV      + D ME+FFL ET+KYL+LLF    G D+++     K+VF+TE H LP
Sbjct:  523 LRPETVESLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGETLKYLYLLF----GDDSVIPLD--KFVFNTEAHPLP 619
[Close]
BLAST of GH720347 vs. TAIR peptide
Match: AT1G51590.2 (| Symbols: MNS1, MANIB | alpha-mannosidase 1 | chr1:19128315-19131406 REVERSE LENGTH=456)

HSP 1 Score: 68.9366 bits (167), Expect = 2.934e-12
Identity = 41/114 (35.96%), Postives = 66/114 (57.89%), Query Frame = -3 
Query:  261 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLL-------PATPQISL 581
            LRPE VES ++L++ T +  Y + G ++  + +  +R   GY  + DV    +++ M+SFFLAET+KYL+LLF     P +++     ++VF+TE H L       P  P I+L
Sbjct:  338 LRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGAKDNKMQSFFLAETLKYLYLLF----SPSSVISLD--EWVFNTEAHPLKIVARNDPRKPTIAL 445
[Close]
BLAST of GH720347 vs. TAIR peptide
Match: AT1G51590.1 (| Symbols: MNS1, MANIB | alpha-mannosidase 1 | chr1:19128315-19132132 REVERSE LENGTH=560)

HSP 1 Score: 68.9366 bits (167), Expect = 2.934e-12
Identity = 41/114 (35.96%), Postives = 66/114 (57.89%), Query Frame = -3 
Query:  261 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLL-------PATPQISL 581
            LRPE VES ++L++ T +  Y + G ++  + +  +R   GY  + DV    +++ M+SFFLAET+KYL+LLF     P +++     ++VF+TE H L       P  P I+L
Sbjct:  442 LRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGAKDNKMQSFFLAETLKYLYLLF----SPSSVISLD--EWVFNTEAHPLKIVARNDPRKPTIAL 549
[Close]
BLAST of GH720347 vs. TAIR peptide
Match: AT3G21160.1 (| Symbols: MNS2, MANIA | alpha-mannosidase 2 | chr3:7414129-7418328 REVERSE LENGTH=572)

HSP 1 Score: 66.6254 bits (161), Expect = 1.456e-11
Identity = 37/99 (37.37%), Postives = 60/99 (60.61%), Query Frame = -3 
Query:  285 LRPELVESTYWLYKATRDPRYLDAGRDMLASLQYGTRCPCGYCHISDVEHHKQEDHMESFFLAETVKYLWLLFDLAVGPDNLVENGPYKYVFSTEGHLL 581
            LRPE VES ++L++ T +  Y + G ++  + +  +R   GY  + DV    +++ M+SFFLAET+KYL+LLF     P  ++     ++VF+TE H L
Sbjct:  443 LRPETVESLFYLWRLTGNKTYQEWGWNIFEAFEKNSRIESGYVGLKDVNTGVKDNKMQSFFLAETLKYLYLLF----SPTTVIPLD--EWVFNTEAHPL 535
The following BLAST results are available for this feature:
BLAST of GH720347 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9RTF7_RICCO5.915e-9074.16Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA,... [more]
D7T783_VITVI1.205e-8772.25Whole genome shotgun sequence of line PN40024, sca... [more]
B9HKD6_POPTR1.720e-8671.29Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q9FG93_ARATH2.058e-8169.67Dbj|BAA91806.1 OS=Arabidopsis thaliana GN=At5g4371... [more]
D7MNM7_ARALY2.934e-8069.38Glycoside hydrolase family 47 protein OS=Arabidops... [more]
Q0JIW3_ORYSJ8.484e-7765.55Os01g0773600 protein OS=Oryza sativa subsp. japoni... [more]
Q5ZBZ0_ORYSJ8.485e-7765.55Putative aspartyl(D) tRNA Synthetase (89.0 kD)(Drs... [more]
B9ET98_ORYSJ8.487e-7765.55Putative uncharacterized protein OS=Oryza sativa s... [more]
B8AA67_ORYSI8.488e-7765.55Putative uncharacterized protein OS=Oryza sativa s... [more]
C5XLL0_SORBI3.439e-7673.30Putative uncharacterized protein Sb03g036080 OS=So... [more]
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BLAST of GH720347 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
EDEM1_HUMAN2.222e-2749.25ER degradation-enhancing alpha-mannosidase-like 1 ... [more]
EDEM1_MOUSE2.903e-2748.51ER degradation-enhancing alpha-mannosidase-like 1 ... [more]
MNL1_SCHPO3.791e-2740.27ER degradation-enhancing alpha-mannosidase-like pr... [more]
EDEM2_HUMAN6.052e-2540.54ER degradation-enhancing alpha-mannosidase-like 2 ... [more]
EDEM3_XENLA9.663e-2344.03ER degradation-enhancing alpha-mannosidase-like 3 ... [more]
EDEM3_MOUSE2.153e-2244.78ER degradation-enhancing alpha-mannosidase-like 3 ... [more]
EDEM3_HUMAN2.153e-2244.78ER degradation-enhancing alpha-mannosidase-like 3 ... [more]
MNL1_YEAST6.481e-1936.99ER degradation-enhancing alpha-mannosidase-like pr... [more]
MA1B1_RAT2.158e-1448.08Endoplasmic reticulum mannosyl-oligosaccharide 1,2... [more]
MA1B1_MOUSE3.680e-1448.08Endoplasmic reticulum mannosyl-oligosaccharide 1,2... [more]
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BLAST of GH720347 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6
Match NameE-valueIdentityDescription
AT5G43710.11.408e-8369.67| Symbols: | Glycosyl hydrolase family 47 protein... [more]
AT1G27520.11.712e-2847.06| Symbols: | Glycosyl hydrolase family 47 protein... [more]
AT1G30000.11.008e-1241.75| Symbols: MNS3 | alpha-mannosidase 3 | chr1:10509... [more]
AT1G51590.22.934e-1235.96| Symbols: MNS1, MANIB | alpha-mannosidase 1 | chr... [more]
AT1G51590.12.934e-1235.96| Symbols: MNS1, MANIB | alpha-mannosidase 1 | chr... [more]
AT3G21160.11.456e-1137.37| Symbols: MNS2, MANIA | alpha-mannosidase 2 | chr... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase, family 47PRINTSPR00747GLYHDRLASE47coord: 39..63
score: 1.2e-11coord: 98..118
score: 1.2
IPR001382Glycoside hydrolase, family 47GENE3D1.50.10.50no descriptioncoord: 10..143
score: 4.3
IPR001382Glycoside hydrolase, family 47PANTHERPTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 6..139
score: 9.8
IPR001382Glycoside hydrolase, family 47PFAMPF01532Glyco_hydro_47coord: 6..139
score: 3.7
IPR001382Glycoside hydrolase, family 47SUPERFAMILY48225Seven-hairpin glycosidasescoord: 3..142
score: 9.7
NoneNo IPR availablePANTHERPTHR11742:SF10MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 6..139
score: 9.8
NoneNo IPR availableSEGsegsegcoord: 187..206
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH720347 ID=GH720347; Name=GH720347; organism=Pisum sativum; type=EST; length=700bp
TAGCGTGGTCGCGGCCGAGGTACAATAAACCAAACTTCTGTCCATGATTT
AGTCCTGGGGCAGAAACCCTTGATCAAACCAGAAGAAGCACTTGCTTCAG
AAGTAGTGTAGTCTGAGGAGTATGTAGATTTAGTCCAGCTCCCATAAAAT
CTACTATCATTTGATTCGTGCTTATCTCTCTCGGCCTCGGACACAAAATA
AGTTTGTCTCAAATCACCACTTCTACAATAAGCTCCGTAGTATAAACAAT
GTTCCCTCACAAGAGAGATTTGAGGAGTAGCAGGCAATAAATGACCTTCA
GTGCTGAATACATACTTGTATGGCCCATTTTCCACAAGGTTATCAGGACC
CACGGCCAAATCAAAAAGGAGCCACAGATACTTCACTGTCTCAGCTAAAA
AGAAGCTCTCCATATGATCTTCTTGTTTGTGATGTTCAACATCTGAAATG
TGACAGTATCCACAAGGGCATCGAGTCCCATACTGTAAACTGGCAAGCAT
GTCCCTCCCAGCATCAAGATATCTAGGATCCCTGGTAGCTTTGTATAGCC
AATATGTGCTCTCTACTAACTCAGGCCGCAGCGGGTAACTTTTTTGCCCA
TGCTGAACACTTAGACTAGCAAGATTAAAGCCCTCGGGGGTAAAACCATA
TCTTCTCCAGACACTCAAGAAGGCAGCATGAGTACCTGCCCGGGCGGCCG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR001382Glyco_hydro_47