AM161991

EST Overview
NameAM161991
Unique NameAM161991
TypeEST
OrganismPisum sativum (pea)
Sequence length295
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of AM161991 vs. TrEMBL
Match: B9IAV1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_573120 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 9.836e-41
Identity = 84/98 (85.71%), Postives = 90/98 (91.84%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWPLELMAYAYLAVSP SM  +FEEMAA ASN+TT+NK + YP+IEEQALQFILDSCE SISKY KFLQASGS+DLDVTSPKQLNRRLFLKQLAVDLC
Sbjct:  366 GWPLELMAYAYLAVSPPSMSRQFEEMAAAASNKTTTNKKITYPDIEEQALQFILDSCELSISKYTKFLQASGSMDLDVTSPKQLNRRLFLKQLAVDLC 463
[Close]
BLAST of AM161991 vs. TrEMBL
Match: B9T1U1_RICCO (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplast, putative OS=Ricinus communis GN=RCOM_0691150 PE=4 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 1.678e-40
Identity = 83/98 (84.69%), Postives = 90/98 (91.84%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWP+EL+AYAYLAVSP SM  KFEE+AA ASN+TT  KD+ +PEIEEQALQFILDSCESSISKY KFLQASGS+DLDVTSPKQLNRRLFLKQLAVDLC
Sbjct:  371 GWPVELLAYAYLAVSPPSMSSKFEELAAAASNKTTIKKDVGFPEIEEQALQFILDSCESSISKYTKFLQASGSMDLDVTSPKQLNRRLFLKQLAVDLC 468
[Close]
BLAST of AM161991 vs. TrEMBL
Match: D7TD93_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_57.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030117001 PE=4 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.191e-40
Identity = 82/98 (83.67%), Postives = 90/98 (91.84%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWPLELMAYAYL VSP SM   FEE+AA ASN+TTS KD+RYPE+EEQALQFILDSCE+SISKY+KFLQ SGS+DLDVTSPKQLNRR+FLKQLAVDLC
Sbjct:  360 GWPLELMAYAYLVVSPPSMSQHFEEIAAVASNKTTSKKDIRYPELEEQALQFILDSCEASISKYSKFLQESGSMDLDVTSPKQLNRRVFLKQLAVDLC 457
[Close]
BLAST of AM161991 vs. TrEMBL
Match: Q9S7D2_ARATH (Putative ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I OS=Arabidopsis thaliana GN=MLP3.12 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.274e-36
Identity = 73/97 (75.26%), Postives = 85/97 (87.63%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDL 292
            GWP+ELMAYAYL VSP  MR  FEEMA  ASN+T++  DL+YPEIEE ALQFILDSCE+SISKY++FL+ SGS+DLD+TSPKQLNR+ FLKQLAVDL
Sbjct:  367 GWPMELMAYAYLVVSPPDMRNNFEEMAKAASNKTSTKNDLKYPEIEEDALQFILDSCETSISKYSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDL 463
[Close]
BLAST of AM161991 vs. TrEMBL
Match: Q8LF17_ARATH (Putative ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.274e-36
Identity = 73/97 (75.26%), Postives = 85/97 (87.63%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDL 292
            GWP+ELMAYAYL VSP  MR  FEEMA  ASN+T++  DL+YPEIEE ALQFILDSCE+SISKY++FL+ SGS+DLD+TSPKQLNR+ FLKQLAVDL
Sbjct:  367 GWPMELMAYAYLVVSPPDMRNNFEEMAKAASNKTSTKNDLKYPEIEEDALQFILDSCETSISKYSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDL 463
[Close]
BLAST of AM161991 vs. TrEMBL
Match: D7L6N3_ARALY (SET domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_896938 PE=4 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 2.772e-35
Identity = 72/97 (74.23%), Postives = 84/97 (86.60%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDL 292
            GWP+ELMAYAYL VSP  M   FEEMA  ASN+T++  DL+YPEIEE ALQFILDSCE+SISKY++FL+ SGS+DLD+TSPKQLNR+ FLKQLAVDL
Sbjct:  367 GWPMELMAYAYLVVSPPDMGNNFEEMAKAASNKTSTKTDLKYPEIEEDALQFILDSCETSISKYSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDL 463
[Close]
BLAST of AM161991 vs. TrEMBL
Match: O65218_ARATH (Putative methyltransferase OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.728e-35
Identity = 72/97 (74.23%), Postives = 84/97 (86.60%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDL 292
            GWP+ELMAYAYL VSP  MR  FEEMA  ASN+T++  DL+YPEIEE ALQFILDSCE+SISK ++FL+ SGS+DLD+TSPKQLNR+ FLKQLAVDL
Sbjct:  367 GWPMELMAYAYLVVSPPDMRNNFEEMAKRASNKTSTKNDLKYPEIEEDALQFILDSCETSISKCSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDL 463
[Close]
BLAST of AM161991 vs. TrEMBL
Match: Q6Z5K4_ORYSJ (Os02g0725200 protein OS=Oryza sativa subsp. japonica GN=P0685G12.43 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 3.629e-27
Identity = 65/99 (65.66%), Postives = 76/99 (76.77%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQ-ASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWP+ELMAYA+L VSP  M  +FEEMA  ASN++ S   L YPE+EEQALQFILD CES+I+KY KFL+ +SGSL L  T+ KQ NR L LKQLA DLC
Sbjct:  365 GWPVELMAYAFLVVSPPEMSQRFEEMAVAASNKSPSKPGLNYPELEEQALQFILDCCESNIAKYTKFLEGSSGSLQLS-TNSKQANRTLLLKQLARDLC 462
[Close]
BLAST of AM161991 vs. TrEMBL
Match: B8AHQ1_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_08761 PE=4 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 3.629e-27
Identity = 65/99 (65.66%), Postives = 76/99 (76.77%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQ-ASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWP+ELMAYA+L VSP  M  +FEEMA  ASN++ S   L YPE+EEQALQFILD CES+I+KY KFL+ +SGSL L  T+ KQ NR L LKQLA DLC
Sbjct:  365 GWPVELMAYAFLVVSPPEMSQRFEEMAVAASNKSPSKPGLNYPELEEQALQFILDCCESNIAKYTKFLEGSSGSLQLS-TNSKQANRTLLLKQLARDLC 462
[Close]
BLAST of AM161991 vs. TrEMBL
Match: B6U6Z1_MAIZE (Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I OS=Zea mays PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 4.436e-25
Identity = 59/98 (60.20%), Postives = 69/98 (70.41%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDLC 295
            GWP+ELMAYA+L VSP  M   FEEMAA ASN+T+S   L YP++EEQALQFILD CES+I KY K+L+          + KQ NR L LKQLA DLC
Sbjct:  366 GWPVELMAYAFLVVSPPDMSQCFEEMAAAASNKTSSKPGLNYPDLEEQALQFILDCCESNIEKYTKYLEGGAGSPEVPMNAKQANRTLLLKQLARDLC 463
[Close]
BLAST of AM161991 vs. TAIR peptide
Match: AT3G07670.1 (| Symbols: | Rubisco methyltransferase family protein | chr3:2451651-2454617 FORWARD LENGTH=504)

HSP 1 Score: 154.451 bits (389), Expect = 1.290e-38
Identity = 73/97 (75.26%), Postives = 85/97 (87.63%), Query Frame = 2 
Query:    2 GWPLELMAYAYLAVSPSSMRGKFEEMAATASNETTSNKDLRYPEIEEQALQFILDSCESSISKYNKFLQASGSLDLDVTSPKQLNRRLFLKQLAVDL 292
            GWP+ELMAYAYL VSP  MR  FEEMA  ASN+T++  DL+YPEIEE ALQFILDSCE+SISKY++FL+ SGS+DLD+TSPKQLNR+ FLKQLAVDL
Sbjct:  367 GWPMELMAYAYLVVSPPDMRNNFEEMAKAASNKTSTKNDLKYPEIEEDALQFILDSCETSISKYSRFLKESGSMDLDITSPKQLNRKAFLKQLAVDL 463
The following BLAST results are available for this feature:
BLAST of AM161991 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9IAV1_POPTR9.836e-4185.71Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9T1U1_RICCO1.678e-4084.69Ribulose-1,5 bisphosphate carboxylase/oxygenase la... [more]
D7TD93_VITVI2.191e-4083.67Whole genome shotgun sequence of line PN40024, sca... [more]
Q9S7D2_ARATH3.274e-3675.26Putative ribulose-1,5-bisphosphate carboxylase/oxy... [more]
Q8LF17_ARATH3.274e-3675.26Putative ribulose-1,5-bisphosphate carboxylase/oxy... [more]
D7L6N3_ARALY2.772e-3574.23SET domain-containing protein OS=Arabidopsis lyrat... [more]
O65218_ARATH4.728e-3574.23Putative methyltransferase OS=Arabidopsis thaliana... [more]
Q6Z5K4_ORYSJ3.629e-2765.66Os02g0725200 protein OS=Oryza sativa subsp. japoni... [more]
B8AHQ1_ORYSI3.629e-2765.66Putative uncharacterized protein OS=Oryza sativa s... [more]
B6U6Z1_MAIZE4.436e-2560.20Ribulose-1,5-bisphosphate carboxylase/oxygenase sm... [more]
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BLAST of AM161991 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G07670.11.290e-3875.26| Symbols: | Rubisco methyltransferase family pro... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015353Rubisco LS methyltransferase, substrate-binding domainPFAMPF09273Rubis-subs-bindcoord: 2..96
score: 1.1

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM161991 ID=AM161991; Name=AM161991; organism=Pisum sativum; type=EST; length=295bp
TGGTTGGCCGTTGGAGTTAATGGCTTATGCTTATTTAGCCGTTAGCCCTT
CAAGCATGAGAGGAAAATTTGAAGAGATGGCTGCAACTGCATCAAATGAA
ACTACCTCCAATAAGGACTTGAGATATCCAGAAATAGAGGAGCAGGCATT
GCAGTTCATACTAGATAGTTGTGAATCTAGTATCTCAAAATACAATAAGT
TCTTACAGGCAAGTGGATCACTGGATTTGGACGTGACTTCTCCGAAACAA
CTTAACCGGAGACTCTTTCTAAAACAGCTAGCCGTGGACTTGTGT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR015353Rubisco_LSMT_subst-bd