AM162019

EST Overview


Name	AM162019
Unique Name	AM162019
Type	EST
Organism	Pisum sativum (pea)
Sequence length	313

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162019 vs. TrEMBL
Match: D7U228_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_128.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00000074001 PE=4 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.873e-47
Identity = 86/103 (83.50%), Postives = 96/103 (93.20%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            ISC+D+GD LSGHVGSP+P CEIKLVDVPEMNYTS+DQP PRGEI +RGP++FQGYYKDEVQT+EVID +GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  451 ISCMDKGDNLSGHVGSPNPACEIKLVDVPEMNYTSEDQPYPRGEICVRGPVLFQGYYKDEVQTKEVIDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 553

[Close]

BLAST of AM162019 vs. TrEMBL
Match: A5AVM2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002313 PE=4 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.873e-47
Identity = 86/103 (83.50%), Postives = 96/103 (93.20%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            ISC+D+GD LSGHVGSP+P CEIKLVDVPEMNYTS+DQP PRGEI +RGP++FQGYYKDEVQT+EVID +GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  451 ISCMDKGDNLSGHVGSPNPACEIKLVDVPEMNYTSEDQPYPRGEICVRGPVLFQGYYKDEVQTKEVIDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 553

[Close]

BLAST of AM162019 vs. TrEMBL
Match: D7L4Q5_ARALY (Long-chain acyl-CoA synthetase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_477894 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 1.586e-46
Identity = 86/103 (83.50%), Postives = 96/103 (93.20%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KLVDVPEMNYTS DQP+PRGEI +RGPIIF+GYYKDEVQT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  455 ISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSVDQPHPRGEICVRGPIIFRGYYKDEVQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 557

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q9C5U7_ARATH (Long-chain acyl-CoA synthetase OS=Arabidopsis thaliana GN=AtLACS6 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 2.071e-46
Identity = 86/103 (83.50%), Postives = 95/103 (92.23%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KLVDVPEMNYTS DQP+PRGEI +RGPIIF GYYKDEVQT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEVQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q8LKS6_ARATH (Long chain acyl-CoA synthetase 6 OS=Arabidopsis thaliana GN=LACS6 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 2.071e-46
Identity = 86/103 (83.50%), Postives = 95/103 (92.23%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CEIKLVDVPEMNYTS DQP+PRGEI +RGPIIF GYYKDE+QT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISGMDEGDNLTGHVGSPNPACEIKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEIQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q9SFG1_ARATH (Putative long-chain-fatty-acid--CoA ligase OS=Arabidopsis thaliana GN=F2O10.7 PE=4 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.704e-46
Identity = 85/103 (82.52%), Postives = 95/103 (92.23%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KLVDVPEMNYTS DQP+PRGEI +RGPIIF GYYKDE+QT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  442 ISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEIQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 544

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q96338_BRANA (AMP-binding protein OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.704e-46
Identity = 85/103 (82.52%), Postives = 96/103 (93.20%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KL+DVPEMNYTS D+P+PRGEI +RGPIIF+GYYKDEVQTREVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  453 ISGMDEGDNLTGHVGSPNPACEVKLMDVPEMNYTSADEPHPRGEICVRGPIIFRGYYKDEVQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 555

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q8LPS1_ARATH (AT3g05970/F2O10_9 OS=Arabidopsis thaliana GN=At3g05970 PE=2 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.704e-46
Identity = 85/103 (82.52%), Postives = 95/103 (92.23%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KLVDVPEMNYTS DQP+PRGEI +RGPIIF GYYKDE+QT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEIQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TrEMBL
Match: Q8LKS5_ARATH (Long chain acyl-CoA synthetase 7 OS=Arabidopsis thaliana GN=LACS7 PE=1 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 4.613e-46
Identity = 84/103 (81.55%), Postives = 94/103 (91.26%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +D+GD LSGHVGSP+P CE+KLVDVPEMNYTSDDQP PRGEI +RGPIIF+GYYKDE QTRE++D +GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISAMDDGDNLSGHVGSPNPACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKDEEQTREILDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TrEMBL
Match: D7M631_ARALY (Long-chain acyl-CoA synthetase 7 OS=Arabidopsis lyrata subsp. lyrata GN=LACS7 PE=4 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 4.613e-46
Identity = 84/103 (81.55%), Postives = 94/103 (91.26%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +D+GD LSGHVGSP+P CE+KLVDVPEMNYTSDDQP PRGEI +RGPIIF+GYYKDE QTRE++D +GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  456 ISAMDDGDNLSGHVGSPNPACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKDEEQTREILDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 558

[Close]

BLAST of AM162019 vs. SwissProt
Match: FCSA_DICDI (Fatty acyl-CoA synthetase A OS=Dictyostelium discoideum GN=fcsA PE=1 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.454e-30
Identity = 60/96 (62.50%), Postives = 72/96 (75.00%), Query Frame = -1

Query:    2 DRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 289
            D   GHVG P   CE+KL+DVPEMNY S D+P PRGE+ IRG  +F+GY+KD  +T+E + E+GW HTGDIG W   G L IIDRKKNIFKL+QGE
Sbjct:  432 DNNVGHVGPPLSACEMKLIDVPEMNYFSTDKPCPRGEVCIRGFNVFKGYFKDPEKTKEDLKEDGWFHTGDIGRWNENGTLSIIDRKKNIFKLSQGE 527

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL1_HUMAN (Long-chain-fatty-acid--CoA ligase 1 OS=Homo sapiens GN=ACSL1 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 3.582e-29
Identity = 59/97 (60.82%), Postives = 73/97 (75.26%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  +GHVG+P PC  IKLVDV EMNY + +     GE+ ++GP +FQGY KD  +T E +D++GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  473 GDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEG---EGEVCVKGPNVFQGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGE 566

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL6_RAT (Long-chain-fatty-acid--CoA ligase 6 OS=Rattus norvegicus GN=Acsl6 PE=1 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 7.979e-29
Identity = 59/97 (60.82%), Postives = 72/97 (74.23%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  SGHVG+P PC  IKLVD  E+NY +       GEI ++GP +F+GY KDE +T+E +D +GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  473 GDWTSGHVGAPLPCNHIKLVDAEELNYWTS---KGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLPEGTLKIIDRKKHIFKLAQGE 566

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL6_HUMAN (Long-chain-fatty-acid--CoA ligase 6 OS=Homo sapiens GN=ACSL6 PE=2 SV=4)

HSP 1 Score: 125.561 bits (314), Expect = 7.979e-29
Identity = 60/97 (61.86%), Postives = 72/97 (74.23%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  SGHVG+P PC  IKLVDV E+NY +       GEI +RGP +F+GY KD  +T+E +D +GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  473 GDWTSGHVGAPLPCNHIKLVDVEELNYWAC---KGEGEICVRGPNVFKGYLKDPDRTKEALDSDGWLHTGDIGKWLPAGTLKIIDRKKHIFKLAQGE 566

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL6_MOUSE (Long-chain-fatty-acid--CoA ligase 6 OS=Mus musculus GN=Acsl6 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.778e-28
Identity = 59/97 (60.82%), Postives = 72/97 (74.23%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  SGHVG+P PC  IKLVD  E+NY +       GEI ++GP +F+GY KDE +T+E +D +GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  473 GDWTSGHVGAPLPCNHIKLVDAEELNYWTC---KGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLPEGTLKIIDRKKHIFKLAQGE 566

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL1_CAVPO (Long-chain-fatty-acid--CoA ligase 1 OS=Cavia porcellus GN=ACSL1 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.322e-28
Identity = 58/97 (59.79%), Postives = 72/97 (74.23%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  +GHVG+P PC  IKLVDV EMNY +       GE+ ++GP +F+GY KD  +T E +D++GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  473 GDWTAGHVGAPMPCNFIKLVDVEEMNYMA---AMGEGEVCVKGPNVFKGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGE 566

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL1_MOUSE (Long-chain-fatty-acid--CoA ligase 1 OS=Mus musculus GN=Acsl1 PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 8.822e-28
Identity = 56/97 (57.73%), Postives = 71/97 (73.20%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  +GHVG+P PC  +KLVDV EMNY +       GE+ ++G  +F+GY KD  +T E +D++GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  474 GDWTAGHVGAPMPCNYVKLVDVEEMNYLAS---KGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGE 567

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL5_RAT (Long-chain-fatty-acid--CoA ligase 5 OS=Rattus norvegicus GN=Acsl5 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.505e-27
Identity = 60/102 (58.82%), Postives = 73/102 (71.57%), Query Frame = -1

Query:    2 SCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 307
            S    GD  +GHVG+P  C  +KL DV +MNY S    N  GEI I+G  +F+GY KD  +T+EV+D++GWLHTGDIG W+  G LKIIDRKKNIFKLAQGE
Sbjct:  453 SITSPGDWTAGHVGTPVSCNFVKLEDVADMNYFS---VNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGE 551

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL5_MOUSE (Long-chain-fatty-acid--CoA ligase 5 OS=Mus musculus GN=Acsl5 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.965e-27
Identity = 59/102 (57.84%), Postives = 73/102 (71.57%), Query Frame = -1

Query:    2 SCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 307
            S    GD  +GHVG+P  C  +KL DV +MNY S    N  GEI I+G  +F+GY KD  +T+EV+D++GWLHTGDIG W+  G LKI+DRKKNIFKLAQGE
Sbjct:  453 SITSPGDWTAGHVGTPVACNFVKLEDVADMNYFS---VNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGE 551

[Close]

BLAST of AM162019 vs. SwissProt
Match: ACSL1_RAT (Long-chain-fatty-acid--CoA ligase 1 OS=Rattus norvegicus GN=Acsl1 PE=1 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.965e-27
Identity = 56/97 (57.73%), Postives = 71/97 (73.20%), Query Frame = -1

Query:    2 GDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 292
            GD  +GHVG+P PC  IKLVDV +MNY +       GE+ ++G  +F+GY KD  +T E +D++GWLHTGDIG W+  G LKIIDRKK+IFKLAQGE
Sbjct:  474 GDWTAGHVGAPMPCNYIKLVDVEDMNYQA---AKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGE 567

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT3G05970.1 (| Symbols: LACS6, ATLACS6 | long-chain acyl-CoA synthetase 6 | chr3:1786510-1791746 REVERSE LENGTH=701)

HSP 1 Score: 187.963 bits (476), Expect = 1.034e-48
Identity = 85/103 (82.52%), Postives = 95/103 (92.23%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +DEGD L+GHVGSP+P CE+KLVDVPEMNYTS DQP+PRGEI +RGPIIF GYYKDE+QT+EVIDE+GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISGMDEGDNLTGHVGSPNPACEVKLVDVPEMNYTSADQPHPRGEICVRGPIIFTGYYKDEIQTKEVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT5G27600.1 (| Symbols: LACS7, ATLACS7 | long-chain acyl-CoA synthetase 7 | chr5:9742616-9746795 FORWARD LENGTH=700)

HSP 1 Score: 187.193 bits (474), Expect = 1.764e-48
Identity = 84/103 (81.55%), Postives = 94/103 (91.26%), Query Frame = -1

Query:    2 ISCIDEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 310
            IS +D+GD LSGHVGSP+P CE+KLVDVPEMNYTSDDQP PRGEI +RGPIIF+GYYKDE QTRE++D +GWLHTGDIG W+ GGRLKIIDRKKNIFKLAQGE
Sbjct:  457 ISAMDDGDNLSGHVGSPNPACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKDEEQTREILDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGE 559

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT4G11030.1 (| Symbols: | AMP-dependent synthetase and ligase family protein | chr4:6738120-6742229 FORWARD LENGTH=666)

HSP 1 Score: 108.997 bits (271), Expect = 6.105e-25
Identity = 60/99 (60.61%), Postives = 68/99 (68.69%), Query Frame = -1

Query:    2 DEGDRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 298
            DE D L G VG P P  +I+L  VPEMNY +     PRGEI IRG  +F GYYK E  T+EV  + GWLHTGD+G W   G +KIIDRKKNIFKLAQGE
Sbjct:  433 DELDML-GTVGPPVPNVDIRLESVPEMNYDALGS-TPRGEICIRGKTLFSGYYKREDLTKEVFID-GWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGE 528

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT1G64400.1 (| Symbols: LACS3 | AMP-dependent synthetase and ligase family protein | chr1:23915802-23919681 REVERSE LENGTH=665)

HSP 1 Score: 105.145 bits (261), Expect = 8.815e-24
Identity = 55/92 (59.78%), Postives = 64/92 (69.57%), Query Frame = -1

Query:    2 GHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 277
            G VG P P  +I+L  VPEM Y +    NPRGEI IRG  +F GYYK E  T+EV  + GWLHTGD+G W   G +KIIDRKKNIFKL+QGE
Sbjct:  439 GTVGPPVPNVDIRLESVPEMGYDALAS-NPRGEICIRGKTLFSGYYKREDLTQEVFID-GWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGE 528

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT1G49430.1 (| Symbols: LACS2, LRD2 | long-chain acyl-CoA synthetase 2 | chr1:18291188-18295641 FORWARD LENGTH=665)

HSP 1 Score: 101.293 bits (251), Expect = 1.273e-22
Identity = 51/92 (55.43%), Postives = 62/92 (67.39%), Query Frame = -1

Query:    2 GHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 277
            G VG P P  E +LV VPEM Y +     PRGEI +RG  +F GY+K +  T +V+ + GW HTGDIG W   G +KIIDRKKNIFKL+QGE
Sbjct:  439 GTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQDLTDQVLID-GWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGE 529

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT4G23850.1 (| Symbols: LACS4 | AMP-dependent synthetase and ligase family protein | chr4:12403720-12408263 REVERSE LENGTH=666)

HSP 1 Score: 100.908 bits (250), Expect = 1.662e-22
Identity = 53/92 (57.61%), Postives = 63/92 (68.48%), Query Frame = -1

Query:    2 GHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 277
            G VG P P  +I+L  VPEM Y +      RGEI IRG  +F GYYK E  T+EV+ + GWLHTGD+G W   G +KIIDRKKNIFKL+QGE
Sbjct:  439 GTVGPPVPNVDIRLESVPEMEYDALAS-TARGEICIRGKTLFSGYYKREDLTKEVLID-GWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGE 528

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT1G77590.1 (| Symbols: LACS9 | long chain acyl-CoA synthetase 9 | chr1:29148501-29151776 REVERSE LENGTH=691)

HSP 1 Score: 95.5153 bits (236), Expect = 6.985e-21
Identity = 52/100 (52.00%), Postives = 66/100 (66.00%), Query Frame = -1

Query:    2 DRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREV--IDEEG--WLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 289
            D   G VG+P PC  +KLVD  E  Y + D+P PRGEI I G  I  GY+K+E +T+EV  +DE+G  W +TGDIG +   G L+IIDRKK+I KL  GE
Sbjct:  463 DTSVGRVGAPLPCSFVKLVDWAEGGYLTSDKPMPRGEIVIGGSNITLGYFKNEEKTKEVYKVDEKGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGE 562

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT2G47240.2 (| Symbols: LACS1 | AMP-dependent synthetase and ligase family protein | chr2:19393835-19397616 FORWARD LENGTH=660)

HSP 1 Score: 92.0485 bits (227), Expect = 7.722e-20
Identity = 50/92 (54.35%), Postives = 62/92 (67.39%), Query Frame = -1

Query:    2 GHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 277
            G VG P+   EI+L +V EM Y    + NP GEI IRG  +F GYYK+   T EV+ ++GW HTGDIG  +  G LKIIDRKKN+ KL+QGE
Sbjct:  436 GTVGIPAVYNEIRLEEVSEMGYDPLGE-NPAGEICIRGQCMFSGYYKNPELTEEVM-KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGE 525

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT2G47240.1 (| Symbols: CER8, LACS1 | AMP-dependent synthetase and ligase family protein | chr2:19393835-19397616 FORWARD LENGTH=660)

HSP 1 Score: 92.0485 bits (227), Expect = 7.722e-20
Identity = 50/92 (54.35%), Postives = 62/92 (67.39%), Query Frame = -1

Query:    2 GHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREVIDEEGWLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 277
            G VG P+   EI+L +V EM Y    + NP GEI IRG  +F GYYK+   T EV+ ++GW HTGDIG  +  G LKIIDRKKN+ KL+QGE
Sbjct:  436 GTVGIPAVYNEIRLEEVSEMGYDPLGE-NPAGEICIRGQCMFSGYYKNPELTEEVM-KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGE 525

[Close]

BLAST of AM162019 vs. TAIR peptide
Match: AT2G04350.2 (| Symbols: LACS8 | AMP-dependent synthetase and ligase family protein | chr2:1516086-1519178 FORWARD LENGTH=720)

HSP 1 Score: 85.5001 bits (210), Expect = 7.228e-18
Identity = 46/100 (46.00%), Postives = 61/100 (61.00%), Query Frame = -1

Query:    2 DRLSGHVGSPSPCCEIKLVDVPEMNYTSDDQPNPRGEIYIRGPIIFQGYYKDEVQTREV--IDEEG--WLHTGDIGTWIAGGRLKIIDRKKNIFKLAQGE 289
            D   G VG P PC  +KLV   E  Y   D+P PRGEI + G  +  GY+ ++ +T EV  +DE+G  W +TGDIG +   G L++IDRKK+I KL  GE
Sbjct:  492 DPAVGRVGPPLPCGYVKLVSWEEGGYRISDKPMPRGEIVVGGNSVTAGYFNNQEKTDEVYKVDEKGTRWFYTGDIGRFHPDGCLEVIDRKKDIVKLQHGE 591

The following BLAST results are available for this feature:

BLAST of AM162019 vs. TrEMBL
BLAST of AM162019 vs. SwissProt
BLAST of AM162019 vs. TAIR peptide

BLAST of AM162019 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7U228_VITVI	1.873e-47	83.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AVM2_VITVI	1.873e-47	83.50	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7L4Q5_ARALY	1.586e-46	83.50	Long-chain acyl-CoA synthetase OS=Arabidopsis lyra...	[more]
Q9C5U7_ARATH	2.071e-46	83.50	Long-chain acyl-CoA synthetase OS=Arabidopsis thal...	[more]
Q8LKS6_ARATH	2.071e-46	83.50	Long chain acyl-CoA synthetase 6 OS=Arabidopsis th...	[more]
Q9SFG1_ARATH	2.704e-46	82.52	Putative long-chain-fatty-acid--CoA ligase OS=Arab...	[more]
Q96338_BRANA	2.704e-46	82.52	AMP-binding protein OS=Brassica napus PE=2 SV=1	[more]
Q8LPS1_ARATH	2.704e-46	82.52	AT3g05970/F2O10_9 OS=Arabidopsis thaliana GN=At3g0...	[more]
Q8LKS5_ARATH	4.613e-46	81.55	Long chain acyl-CoA synthetase 7 OS=Arabidopsis th...	[more]
D7M631_ARALY	4.613e-46	81.55	Long-chain acyl-CoA synthetase 7 OS=Arabidopsis ly...	[more]

BLAST of AM162019 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
FCSA_DICDI	1.454e-30	62.50	Fatty acyl-CoA synthetase A OS=Dictyostelium disco...	[more]
ACSL1_HUMAN	3.582e-29	60.82	Long-chain-fatty-acid--CoA ligase 1 OS=Homo sapien...	[more]
ACSL6_RAT	7.979e-29	60.82	Long-chain-fatty-acid--CoA ligase 6 OS=Rattus norv...	[more]
ACSL6_HUMAN	7.979e-29	61.86	Long-chain-fatty-acid--CoA ligase 6 OS=Homo sapien...	[more]
ACSL6_MOUSE	1.778e-28	60.82	Long-chain-fatty-acid--CoA ligase 6 OS=Mus musculu...	[more]
ACSL1_CAVPO	2.322e-28	59.79	Long-chain-fatty-acid--CoA ligase 1 OS=Cavia porce...	[more]
ACSL1_MOUSE	8.822e-28	57.73	Long-chain-fatty-acid--CoA ligase 1 OS=Mus musculu...	[more]
ACSL5_RAT	1.505e-27	58.82	Long-chain-fatty-acid--CoA ligase 5 OS=Rattus norv...	[more]
ACSL5_MOUSE	1.965e-27	57.84	Long-chain-fatty-acid--CoA ligase 5 OS=Mus musculu...	[more]
ACSL1_RAT	1.965e-27	57.73	Long-chain-fatty-acid--CoA ligase 1 OS=Rattus norv...	[more]

BLAST of AM162019 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G05970.1	1.034e-48	82.52	\| Symbols: LACS6, ATLACS6 \| long-chain acyl-CoA sy...	[more]
AT5G27600.1	1.764e-48	81.55	\| Symbols: LACS7, ATLACS7 \| long-chain acyl-CoA sy...	[more]
AT4G11030.1	6.105e-25	60.61	\| Symbols: \| AMP-dependent synthetase and ligase ...	[more]
AT1G64400.1	8.815e-24	59.78	\| Symbols: LACS3 \| AMP-dependent synthetase and li...	[more]
AT1G49430.1	1.273e-22	55.43	\| Symbols: LACS2, LRD2 \| long-chain acyl-CoA synth...	[more]
AT4G23850.1	1.662e-22	57.61	\| Symbols: LACS4 \| AMP-dependent synthetase and li...	[more]
AT1G77590.1	6.985e-21	52.00	\| Symbols: LACS9 \| long chain acyl-CoA synthetase ...	[more]
AT2G47240.2	7.722e-20	54.35	\| Symbols: LACS1 \| AMP-dependent synthetase and li...	[more]
AT2G47240.1	7.722e-20	54.35	\| Symbols: CER8, LACS1 \| AMP-dependent synthetase ...	[more]
AT2G04350.2	7.228e-18	46.00	\| Symbols: LACS8 \| AMP-dependent synthetase and li...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 7..104 score: 4.7
None	No IPR available	GENE3D	2.30.38.10	no description	coord: 13..90 score: 3.9
None	No IPR available	PANTHER	PTHR11968	ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBER	coord: 6..104 score: 3.5
None	No IPR available	PANTHER	PTHR11968:SF8	LONG-CHAIN-FATTY-ACID COA LIGASE	coord: 6..104 score: 3.5
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 1..104 score: 4.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162019 ID=AM162019; Name=AM162019; organism=Pisum sativum; type=EST; length=313bp
ACTCGCCTTGTGCCAACTTAAAGATATTCTTCTTCCTATCAATGATTTTG
AGACGACCACCAGCTATCCATGTCCCAATATCTCCGGTATGCAGCCATCC
CTCTTCGTCAATGACTTCTCTCGTTTGAACTTCATCTTTGTAATATCCTT
GAAAAATAATGGGACCCCTGATGTAAATTTCACCACGGGGGTTCGGCTGA
TCATCAGATGTATAGTTCATTTCCGGAACATCTACAAGTTTTATCTCACA
GCATGGACTTGGGGAGCCAACGTGACCACTAAGTCTGTCACCCTCATCAA
TGCAGCTTATAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig_com

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