EX568953

EST Overview
NameEX568953
Unique NameEX568953
TypeEST
OrganismPisum sativum (pea)
Sequence length460
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of EX568953 vs. SwissProt
Match: RL14_PEA (Probable 60S ribosomal protein L14 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 5.430e-7
Identity = 25/26 (96.15%), Postives = 25/26 (96.15%), Query Frame = 1 
Query:  154 RAALNDFDRFKIMLAKIKRAASVRQE 231
            RAALNDFDRFKIMLAKIKRAA VRQE
Sbjct:   99 RAALNDFDRFKIMLAKIKRAAGVRQE 124

HSP 2 Score: 22.3274 bits (46), Expect = 5.430e-7
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 2 
Query:  125 WGRKLIVRK 151
            WGRKLIV+K
Sbjct:   89 WGRKLIVKK 97
[Close]
BLAST of EX568953 vs. TAIR peptide
Match: AT2G20450.1 (| Symbols: | Ribosomal protein L14 | chr2:8813923-8815071 FORWARD LENGTH=134)

HSP 1 Score: 50.8322 bits (120), Expect = 3.796e-7
Identity = 30/54 (55.56%), Postives = 36/54 (66.67%), Query Frame = 1 
Query:   70 IEKYNLPNFKSKTKKQC*MGKETDC*EERAALNDFDRFKIMLAKIKRAASVRQE 231
            IE     + K+K +K    G++    + RAALNDFDRFKIMLAKIKRA  VRQE
Sbjct:   72 IEAMEKADVKNKWEKSS-WGRKLIVQKRRAALNDFDRFKIMLAKIKRAGIVRQE 124
[Close]
BLAST of EX568953 vs. TAIR peptide
Match: AT2G20450.1 (| Symbols: | Ribosomal protein L14 | chr2:8813923-8815071 FORWARD LENGTH=134)

HSP 1 Score: 50.8322 bits (120), Expect = 3.796e-7
Identity = 30/54 (55.56%), Postives = 36/54 (66.67%), Query Frame = 1 
Query:   70 IEKYNLPNFKSKTKKQC*MGKETDC*EERAALNDFDRFKIMLAKIKRAASVRQE 231
            IE     + K+K +K    G++    + RAALNDFDRFKIMLAKIKRA  VRQE
Sbjct:   72 IEAMEKADVKNKWEKSS-WGRKLIVQKRRAALNDFDRFKIMLAKIKRAGIVRQE 124
[Close]
BLAST of EX568953 vs. Lotus protein
Match: chr6.LjT09C09.120.r2.a (- phase: 0 /partial)

HSP 1 Score: 48.521 bits (114), Expect = 2.912e-8
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 1 
Query:  148 EERAALNDFDRFKIMLAKIKRAASVRQE 231
            + RAALNDFDRFKIML KIKRA +VRQE
Sbjct:   96 KRRAALNDFDRFKIMLTKIKRATAVRQE 123

HSP 2 Score: 25.0238 bits (53), Expect = 2.912e-8
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 2 
Query:   98 KAKQKNSVEWGRKLIVRKK 154
            K K +NS  WGRKLIV+K+
Sbjct:   80 KNKWENS-SWGRKLIVKKR 97
[Close]
BLAST of EX568953 vs. Soybean peptides
Match: Glyma02g00680.1|PACid:16246520 ()

HSP 1 Score: 49.2914 bits (116), Expect = 1.211e-7
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 1 
Query:  148 EERAALNDFDRFKIMLAKIKRAASVRQE 231
            + RA+LNDFDRFKIMLAKIKRAA VRQE
Sbjct:   97 KRRASLNDFDRFKIMLAKIKRAAVVRQE 124

HSP 2 Score: 23.483 bits (49), Expect = 1.211e-7
Identity = 13/30 (43.33%), Postives = 18/30 (60.00%), Query Frame = 2 
Query:   74 KNITFPISKAKQKNSVE---WGRKLIVRKK 154
            K++   +  A  KN  E   WGRKLIV+K+
Sbjct:   69 KDLIQAMEAADVKNRWEKSSWGRKLIVQKR 98
[Close]
BLAST of EX568953 vs. Soybean peptides
Match: Glyma10g00680.1|PACid:16278130 ()

HSP 1 Score: 49.2914 bits (116), Expect = 1.212e-7
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 1 
Query:  148 EERAALNDFDRFKIMLAKIKRAASVRQE 231
            + RA+LNDFDRFKIMLAKIKRAA VRQE
Sbjct:   97 KRRASLNDFDRFKIMLAKIKRAAVVRQE 124

HSP 2 Score: 23.483 bits (49), Expect = 1.212e-7
Identity = 13/30 (43.33%), Postives = 18/30 (60.00%), Query Frame = 2 
Query:   74 KNITFPISKAKQKNSVE---WGRKLIVRKK 154
            K++   +  A  KN  E   WGRKLIV+K+
Sbjct:   69 KDLIQAMEAADVKNRWEKSSWGRKLIVQKR 98
[Close]
BLAST of EX568953 vs. SwissProt
Match: RL14_PEA (Probable 60S ribosomal protein L14 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 5.529e-7
Identity = 25/26 (96.15%), Postives = 25/26 (96.15%), Query Frame = 1 
Query:  154 RAALNDFDRFKIMLAKIKRAASVRQE 231
            RAALNDFDRFKIMLAKIKRAA VRQE
Sbjct:   99 RAALNDFDRFKIMLAKIKRAAGVRQE 124

HSP 2 Score: 22.3274 bits (46), Expect = 5.529e-7
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 2 
Query:  125 WGRKLIVRK 151
            WGRKLIV+K
Sbjct:   89 WGRKLIVKK 97
[Close]
BLAST of EX568953 vs. Medicago proteins
Match: IMGA|Medtr4g120770.1 (60S ribosomal protein L14-1 (AHRD V1 ***- Q9SIM4); contains Interpro domain(s) IPR002784 Ribosomal protein L14 chr04_pseudomolecule_IMGAG_V3.5 41745835-41749386 E EGN_Mt100125 20100825)

HSP 1 Score: 49.6766 bits (117), Expect = 7.441e-7
Identity = 29/54 (53.70%), Postives = 36/54 (66.67%), Query Frame = 1 
Query:   70 IEKYNLPNFKSKTKKQC*MGKETDC*EERAALNDFDRFKIMLAKIKRAASVRQE 231
            I+     + K+K +K    G++    + RAALNDFDRFKIMLAKIKRA  VRQE
Sbjct:   72 IKAMEAADVKNKWEKSS-WGRKLIVKKRRAALNDFDRFKIMLAKIKRAGVVRQE 124
[Close]
BLAST of EX568953 vs. Medicago proteins
Match: IMGA|Medtr1g075720.1 (60S ribosomal protein L14-1 (AHRD V1 ***- Q9SIM4); contains Interpro domain(s) IPR002784 Ribosomal protein L14 chr01_pseudomolecule_IMGAG_V3.5 19277509-19279108 E EGN_Mt100125 20100825)

HSP 1 Score: 49.2914 bits (116), Expect = 9.718e-7
Identity = 28/45 (62.22%), Postives = 33/45 (73.33%), Query Frame = 1 
Query:   97 KSKTKKQC*MGKETDC*EERAALNDFDRFKIMLAKIKRAASVRQE 231
            K+K +K    G++    + RAALNDFDRFKIMLAKIKRA  VRQE
Sbjct:   81 KNKWEKSS-WGRKLIVKKRRAALNDFDRFKIMLAKIKRAGVVRQE 124
The following BLAST results are available for this feature:
BLAST of EX568953 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
RL14_PEA5.430e-796.15Probable 60S ribosomal protein L14 OS=Pisum sativu... [more]
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BLAST of EX568953 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT2G20450.13.796e-755.56| Symbols: | Ribosomal protein L14 | chr2:8813923... [more]
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BLAST of EX568953 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT2G20450.13.796e-755.56| Symbols: | Ribosomal protein L14 | chr2:8813923... [more]
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BLAST of EX568953 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
chr6.LjT09C09.120.r2.a2.912e-882.14- phase: 0 /partial[more]
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BLAST of EX568953 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2
Match NameE-valueIdentityDescription
Glyma02g00680.1|PACid:162465201.211e-785.71[more]
Glyma10g00680.1|PACid:162781301.212e-785.71[more]
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BLAST of EX568953 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
RL14_PEA5.529e-796.15Probable 60S ribosomal protein L14 OS=Pisum sativu... [more]
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BLAST of EX568953 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 2
Match NameE-valueIdentityDescription
IMGA|Medtr4g120770.17.441e-753.7060S ribosomal protein L14-1 (AHRD V1 ***- Q9SIM4);... [more]
IMGA|Medtr1g075720.19.718e-762.2260S ribosomal protein L14-1 (AHRD V1 ***- Q9SIM4);... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX568953 ID=EX568953; Name=EX568953; organism=Pisum sativum; type=EST; length=460bp
CCTTGCGGTATCCCGATCAGGTACGTAAATTAAACTCAACCTGCAGAAAA
GAGATAACACCCAAGCAGAATAGAAAAATATAACCTTCCCAATTTCAAAA
GCAAAACAAAAAAACAGTGTTGAATGGGGAAGGAAACTGATTGTTAGGAA
GAAAGGGCTGCACTGAATGATTTTGACAGGTTCAAGATTATGTTGGCAAA
GATTAAGAGGGCTGCCAGTGTCAGGCAAGAACTTGCCAAGCTAAGGAAGA
CTGCAGCTTAGGCATTACTTGATACATTTTTCAAATTTTGTTGTCAATTT
TCTCTATTTTAGTACTACTTTTGATGTTTGAGTGTTTTGTATCATTAAGA
CAAGTGGTTACATGTTATGTTTATTTCTTTTTAATTCGACTTAATTTGAC
CGAGTCTAAATAATTTTTTGCAAGAAGCTTGTTGTGAATGATATTATATT
GTTACTAATT
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