FG531971

EST Overview
NameFG531971
Unique NameFG531971
TypeEST
OrganismPisum sativum (pea)
Sequence length415
Libraries
Library NameType
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of FG531971 vs. TrEMBL
Match: B9HEG0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_764631 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.529e-28
Identity = 63/74 (85.14%), Postives = 68/74 (91.89%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            LGQL +HY+AVKFKRLYSS+TGRKLY SVVKGLEPDSVDA+LKYAKEILKW EEK NF+ SEYSMYSTEASS S
Sbjct:  827 LGQLGAHYMAVKFKRLYSSLTGRKLYTSVVKGLEPDSVDAALKYAKEILKWDEEKPNFSLSEYSMYSTEASSYS 900
[Close]
BLAST of FG531971 vs. TrEMBL
Match: B9S8Q3_RICCO (GTP-binding protein alpha subunit, gna, putative OS=Ricinus communis GN=RCOM_0603610 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.388e-26
Identity = 59/74 (79.73%), Postives = 67/74 (90.54%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            LGQL  HYIAVKFK+LY+S+TG+KLYVS+VKGLEPDSVDASLKYA+EILKW EE+ NF+ SEYS YSTEASS S
Sbjct:  843 LGQLGFHYIAVKFKKLYASLTGKKLYVSMVKGLEPDSVDASLKYAREILKWDEERHNFSLSEYSFYSTEASSYS 916
[Close]
BLAST of FG531971 vs. TrEMBL
Match: B9H6B4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831419 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 8.995e-26
Identity = 57/74 (77.03%), Postives = 66/74 (89.19%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            LG L +HY+AVKFKRLY+ +TGRKLY SVVKGLEPDSVDA+LKYA+EI+KW EEK NF+ SEYS+YSTEASS S
Sbjct:  812 LGHLGAHYMAVKFKRLYALLTGRKLYASVVKGLEPDSVDAALKYAREIMKWDEEKPNFSLSEYSLYSTEASSYS 885
[Close]
BLAST of FG531971 vs. TrEMBL
Match: A9PHQ7_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.894e-24
Identity = 57/66 (86.36%), Postives = 61/66 (92.42%), Query Frame = 3 
Query:   69 IAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            +AVKFKRLYSS+TGRKLY SVVKGLEPDSVDA+LKYAKEILKW EEK NF+ SEYSMYSTEASS S
Sbjct:    1 MAVKFKRLYSSLTGRKLYTSVVKGLEPDSVDAALKYAKEILKWDEEKPNFSLSEYSMYSTEASSYS 66
[Close]
BLAST of FG531971 vs. TrEMBL
Match: D7UE13_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_255.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005090001 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.708e-22
Identity = 55/75 (73.33%), Postives = 65/75 (86.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA HYIAV+FK LYSS+TGRKLYVS+VKGLE +SVD +LKYA+EILKW EE+ NF+ S+ S+YSTE SS SH
Sbjct:  682 LGQLAFHYIAVRFKTLYSSLTGRKLYVSLVKGLELNSVDETLKYAREILKWDEERANFSLSD-SVYSTEPSSFSH 755
[Close]
BLAST of FG531971 vs. TrEMBL
Match: A5AKX9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002365 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.708e-22
Identity = 55/75 (73.33%), Postives = 65/75 (86.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA HYIAV+FK LYSS+TGRKLYVS+VKGLE +SVD +LKYA+EILKW EE+ NF+ S+ S+YSTE SS SH
Sbjct:  983 LGQLAFHYIAVRFKTLYSSLTGRKLYVSLVKGLELNSVDETLKYAREILKWDEERANFSLSD-SVYSTEPSSFSH 1056
[Close]
BLAST of FG531971 vs. TrEMBL
Match: O81225_ARATH (Extra-large G-protein OS=Arabidopsis thaliana GN=XLG PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.994e-21
Identity = 52/75 (69.33%), Postives = 62/75 (82.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA H++AVKFKR YSS+TG+KL+VS  K L+P+SVD+SLK A EILKWSEE+ N   SEYSMYSTE SS S+
Sbjct:  814 LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888
[Close]
BLAST of FG531971 vs. TrEMBL
Match: O80462_ARATH (Extra-large G-protein OS=Arabidopsis thaliana GN=XLG PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 2.994e-21
Identity = 52/75 (69.33%), Postives = 62/75 (82.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA H++AVKFKR YSS+TG+KL+VS  K L+P+SVD+SLK A EILKWSEE+ N   SEYSMYSTE SS S+
Sbjct:  814 LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888
[Close]
BLAST of FG531971 vs. TrEMBL
Match: D7LFC2_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_667596 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.911e-21
Identity = 52/75 (69.33%), Postives = 62/75 (82.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA H++AVKFKR YSS+TG+KL+VS  K L+P+SVD+SLK A EILKWSEE+ N   SEYSMYSTE SS S+
Sbjct:  817 LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICLSEYSMYSTEPSSFSN 891
[Close]
BLAST of FG531971 vs. TrEMBL
Match: B9N4S6_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_267688 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.221e-16
Identity = 43/74 (58.11%), Postives = 56/74 (75.68%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            L + AS YIAVKFKRL+  +T RKLYVS+  GLEPD+VD + KYA+E+LKW +E+ N+ ++E S  S EASS S
Sbjct:  632 LARRASQYIAVKFKRLFRDLTDRKLYVSLATGLEPDNVDEAFKYAREVLKWKQEELNYPNNELSSTSIEASSSS 705
[Close]
BLAST of FG531971 vs. TAIR peptide
Match: AT2G23460.1 (| Symbols: XLG1, ATXLG1 | extra-large G-protein 1 | chr2:9995699-9998945 FORWARD LENGTH=888)

HSP 1 Score: 104.76 bits (260), Expect = 1.718e-23
Identity = 52/75 (69.33%), Postives = 62/75 (82.67%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLSH 269
            LGQLA H++AVKFKR YSS+TG+KL+VS  K L+P+SVD+SLK A EILKWSEE+ N   SEYSMYSTE SS S+
Sbjct:  814 LGQLAFHFMAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSFSN 888
[Close]
BLAST of FG531971 vs. TAIR peptide
Match: AT1G31930.3 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 63.1586 bits (152), Expect = 5.731e-11
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNF---NSSEYSMYSTEASS 260
            L   A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW EEK+        E S YST+ SS
Sbjct:  767 LAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841
[Close]
BLAST of FG531971 vs. TAIR peptide
Match: AT1G31930.2 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 63.1586 bits (152), Expect = 5.731e-11
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNF---NSSEYSMYSTEASS 260
            L   A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW EEK+        E S YST+ SS
Sbjct:  767 LAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841
[Close]
BLAST of FG531971 vs. TAIR peptide
Match: AT1G31930.1 (| Symbols: XLG3 | extra-large GTP-binding protein 3 | chr1:11465832-11468961 FORWARD LENGTH=848)

HSP 1 Score: 63.1586 bits (152), Expect = 5.731e-11
Identity = 33/75 (44.00%), Postives = 45/75 (60.00%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVTGRKLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNF---NSSEYSMYSTEASS 260
            L   A  Y+A+KFK LY S+TG+KL+V   +  +  +VD   KY +E+LKW EEK+        E S YST+ SS
Sbjct:  767 LAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFKYVREVLKWDEEKEESYLNGGGEDSFYSTDMSS 841
[Close]
BLAST of FG531971 vs. TAIR peptide
Match: AT4G34390.1 (| Symbols: XLG2 | extra-large GTP-binding protein 2 | chr4:16441579-16444840 FORWARD LENGTH=861)

HSP 1 Score: 56.6102 bits (135), Expect = 5.364e-9
Identity = 40/84 (47.62%), Postives = 49/84 (58.33%), Query Frame = 3 
Query:   45 LGQLASHYIAVKFKRLYSSVT------GR----KLYVSVVKGLEPDSVDASLKYAKEILKWSEEKQNFNSSEYSMYSTEASSLS 266
            + Q A HYI  KFKRLY S+       GR    KL+V  V  LE D+VD +L+YA+EILKW  E+ +    E S  S EASS S
Sbjct:  780 MAQRAFHYIGYKFKRLYDSILEPVNMRGRSFKPKLFVCQVS-LESDTVDNALRYAREILKWHVEETSM-FQEMSTTSIEASSSS 861
The following BLAST results are available for this feature:
BLAST of FG531971 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9HEG0_POPTR2.529e-2885.14Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9S8Q3_RICCO1.388e-2679.73GTP-binding protein alpha subunit, gna, putative O... [more]
B9H6B4_POPTR8.995e-2677.03Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
A9PHQ7_POPTR2.894e-2486.36Putative uncharacterized protein OS=Populus tricho... [more]
D7UE13_VITVI2.708e-2273.33Whole genome shotgun sequence of line PN40024, sca... [more]
A5AKX9_VITVI2.708e-2273.33Putative uncharacterized protein OS=Vitis vinifera... [more]
O81225_ARATH2.994e-2169.33Extra-large G-protein OS=Arabidopsis thaliana GN=X... [more]
O80462_ARATH2.994e-2169.33Extra-large G-protein OS=Arabidopsis thaliana GN=X... [more]
D7LFC2_ARALY3.911e-2169.33Predicted protein OS=Arabidopsis lyrata subsp. lyr... [more]
B9N4S6_POPTR2.221e-1658.11Predicted protein (Fragment) OS=Populus trichocarp... [more]
back to top
BLAST of FG531971 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5
Match NameE-valueIdentityDescription
AT2G23460.11.718e-2369.33| Symbols: XLG1, ATXLG1 | extra-large G-protein 1 ... [more]
AT1G31930.35.731e-1144.00| Symbols: XLG3 | extra-large GTP-binding protein ... [more]
AT1G31930.25.731e-1144.00| Symbols: XLG3 | extra-large GTP-binding protein ... [more]
AT1G31930.15.731e-1144.00| Symbols: XLG3 | extra-large GTP-binding protein ... [more]
AT4G34390.15.364e-947.62| Symbols: XLG2 | extra-large GTP-binding protein ... [more]
back to top
InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitPANTHERPTHR10218GTP-BINDING PROTEIN ALPHA SUBUNITcoord: 10..40
score: 9
NoneNo IPR availablePANTHERPTHR10218:SF90EXTRA-LARGE G-PROTEINcoord: 10..40
score: 9
NoneNo IPR availableSEGsegsegcoord: 3..14
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG531971 ID=FG531971; Name=FG531971; organism=Pisum sativum; type=EST; length=415bp
AACGCACCAACAGCAATAGTAACAGCATTAATAGTAACCCCTCCCTTGGT
CAGCTTGCTTCCCATTACATAGCGGTTAAGTTTAAAAGGCTTTATTCATC
GGTTACGGGACGAAAGTTGTATGTTTCTGTGGTGAAAGGGTTGGAACCAG
ATAGTGTTGATGCATCCCTTAAATATGCCAAGGAAATTTTGAAGTGGAGT
GAAGAGAAGCAAAACTTTAACTCAAGTGAGTACTCGATGTATAGCACTGA
GGCGAGTTCATTATCTCATTGATTGCTAGTTCAGGCATGAGTTAGGAGTC
ATACTTGTGTATGACTTGAATTCTGTCCTTGTACATATATTGCTAACTAG
GAGTAACAGATAATGTAACTTCTACAAGCTATCAACTTGTAAGATGCACT
AGGCACGGGTAGGTA
back to top
Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004871obsolete signal transducer activity
GO:0019001guanyl nucleotide binding
Vocabulary: Biological Process
TermDefinition
GO:0007186G protein-coupled receptor signaling pathway
Vocabulary: INTERPRO
TermDefinition
IPR001019Gprotein_alpha_su