FG532534

EST Overview
NameFG532534
Unique NameFG532534
TypeEST
OrganismPisum sativum (pea)
Sequence length443
Libraries
Library NameType
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of FG532534 vs. TrEMBL
Match: D7TJ61_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033832001 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.848e-18
Identity = 48/72 (66.67%), Postives = 59/72 (81.94%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSCHL 218
            RSRARKQAYTVELEAELNQL+EEN+ L+QALA+ E +R+QQ LEE     QTKA+KA+EKL+ +R+  SC L
Sbjct:  282 RSRARKQAYTVELEAELNQLKEENTLLQQALADFERKRKQQYLEELKMKTQTKAEKAKEKLKKMRKTWSCPL 353
[Close]
BLAST of FG532534 vs. TrEMBL
Match: B9HBJ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_801922 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.414e-18
Identity = 49/72 (68.06%), Postives = 57/72 (79.17%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSCHL 218
            RSRARKQAYTVELEAELNQL+EEN QLK  LAELE +R+QQ  EE+    +TKA K +EKLR  RR++SC L
Sbjct:  302 RSRARKQAYTVELEAELNQLKEENKQLKHDLAELERKRKQQYFEESRMKARTKAHKTKEKLRLTRRSSSCPL 373
[Close]
BLAST of FG532534 vs. TrEMBL
Match: O23964_HELAN (Dc3 promoter-binding factor-1 OS=Helianthus annuus GN=DPBF-1 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.565e-17
Identity = 48/70 (68.57%), Postives = 55/70 (78.57%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSC 212
            RSRARKQAYTVELEAELN L+EEN+QLKQALAE+E +R+QQ  EE      TK QK R+K R LRR +SC
Sbjct:  307 RSRARKQAYTVELEAELNMLKEENAQLKQALAEIERKRKQQFSEEIRMKGVTKCQKVRDKSRMLRRTSSC 376
[Close]
BLAST of FG532534 vs. TrEMBL
Match: D7LIQ2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_321332 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.014e-16
Identity = 47/72 (65.28%), Postives = 53/72 (73.61%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSCHL 218
            RSRARKQAYTVELEAELNQL+EEN+QLK ALAELE +R+QQ  E      Q K  K+  +LR L RN SC L
Sbjct:  368 RSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKTRAQPKLPKSNGRLRTLMRNPSCPL 439
[Close]
BLAST of FG532534 vs. TrEMBL
Match: B9HQ00_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_767006 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.676e-11
Identity = 36/69 (52.17%), Postives = 52/69 (75.36%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTS 209
            RSRARKQAYTVELE ELNQL+EEN++LKQ + E+E +R+++ +   ++ +    Q+  +KLRG+RR  S
Sbjct:  282 RSRARKQAYTVELELELNQLKEENAKLKQIVEEIEEKRKEEVMRRKSSKM---TQEKDDKLRGIRRTVS 347
[Close]
BLAST of FG532534 vs. TrEMBL
Match: B3U2B5_CUCSA (Abscisic acid insensitive OS=Cucumis sativus PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.858e-11
Identity = 36/50 (72.00%), Postives = 43/50 (86.00%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATV 152
            RSRARKQAYTVELEAELNQL+EEN+ LKQALAELE +R+QQ  +  + T+
Sbjct:  373 RSRARKQAYTVELEAELNQLKEENAHLKQALAELERKRKQQPFQFLSTTL 422
[Close]
BLAST of FG532534 vs. TrEMBL
Match: D7LNS0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_484862 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.201e-9
Identity = 36/69 (52.17%), Postives = 48/69 (69.57%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTS 209
            RSRAR+QAYTVELE ELN L EEN++LK+ + E E +RRQ+ +   N + Q   +K  +KLR +RR  S
Sbjct:  264 RSRARRQAYTVELELELNNLTEENTKLKEIVEENEKKRRQEII---NRSKQVTKEKKGDKLRKIRRMAS 329
[Close]
BLAST of FG532534 vs. TrEMBL
Match: B9SRW9_RICCO (DNA binding protein, putative OS=Ricinus communis GN=RCOM_0516810 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.201e-9
Identity = 32/41 (78.05%), Postives = 36/41 (87.80%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQ 125
            RSRARKQAYTVELEAELNQL+EEN QL+  L ELE +R+QQ
Sbjct:  362 RSRARKQAYTVELEAELNQLKEENKQLRHVLTELERKRKQQ 402
[Close]
BLAST of FG532534 vs. TrEMBL
Match: B9SCS5_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1280780 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.494e-9
Identity = 35/69 (50.72%), Postives = 47/69 (68.12%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTS 209
            RSRARKQAYTVELE ELNQL+EEN++LK  + E+E  R+++ L      +    +K  +KLR +RR  S
Sbjct:  290 RSRARKQAYTVELELELNQLKEENAKLKLLVEEIEQNRKEEVLRRKPLIM---PKKKVDKLRSIRRTVS 355
[Close]
BLAST of FG532534 vs. TrEMBL
Match: A5B2U1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009883 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.264e-8
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAE--LEARRRQQ 125
            RSRARKQAYTVELEAELNQL+EEN+ L+QALAE   E +R+QQ
Sbjct:  329 RSRARKQAYTVELEAELNQLKEENTLLQQALAEADFERKRKQQ 371
[Close]
BLAST of FG532534 vs. SwissProt
Match: ABI5_ARATH (Protein ABSCISIC ACID-INSENSITIVE 5 OS=Arabidopsis thaliana GN=ABI5 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.334e-18
Identity = 47/72 (65.28%), Postives = 54/72 (75.00%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSCHL 218
            RSRARKQAYTVELEAELNQL+EEN+QLK ALAELE +R+QQ  E   +  Q K  K+  +LR L RN SC L
Sbjct:  371 RSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL 442
[Close]
BLAST of FG532534 vs. SwissProt
Match: AI5L1_ARATH (ABSCISIC ACID-INSENSITIVE 5-like protein 1 OS=Arabidopsis thaliana GN=DPBF2 PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.754e-11
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTS 209
            RSRAR+QAYTVELE ELN L EEN++LK+ + E E +RRQ+ +  +    Q   +K+ +KLR +RR  S
Sbjct:  263 RSRARRQAYTVELELELNNLTEENTKLKEIVEENEKKRRQEIISRSK---QVTKEKSGDKLRKIRRMAS 328
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT2G36270.1 (| Symbols: ABI5, GIA1 | Basic-leucine zipper (bZIP) transcription factor family protein | chr2:15204980-15206571 REVERSE LENGTH=442)

HSP 1 Score: 90.1225 bits (222), Expect = 5.200e-19
Identity = 47/72 (65.28%), Postives = 54/72 (75.00%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTSCHL 218
            RSRARKQAYTVELEAELNQL+EEN+QLK ALAELE +R+QQ  E   +  Q K  K+  +LR L RN SC L
Sbjct:  371 RSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL 442
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT3G44460.1 (| Symbols: DPBF2, AtbZIP67 | Basic-leucine zipper (bZIP) transcription factor family protein | chr3:16080115-16081722 REVERSE LENGTH=331)

HSP 1 Score: 65.855 bits (159), Expect = 1.050e-11
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRGLRRNTS 209
            RSRAR+QAYTVELE ELN L EEN++LK+ + E E +RRQ+ +  +    Q   +K+ +KLR +RR  S
Sbjct:  263 RSRARRQAYTVELELELNNLTEENTKLKEIVEENEKKRRQEIISRSK---QVTKEKSGDKLRKIRRMAS 328
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT3G19290.3 (| Symbols: ABF4 | ABRE binding factor 4 | chr3:6687956-6689784 FORWARD LENGTH=432)

HSP 1 Score: 50.8322 bits (120), Expect = 3.496e-7
Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAEL-EARRRQQCLEETNATVQTKAQKAREKLRG 191
            RSRARKQAYT+ELEAE+ +L++ N +L++  AE+ E ++ +Q  E +     +K Q  R  L G
Sbjct:  367 RSRARKQAYTLELEAEIEKLKKTNQELQKKQAEMVEMQKNEQLKETSKRPWGSKRQCLRRTLTG 430
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT4G34000.2 (| Symbols: ABF3, DPBF5 | abscisic acid responsive elements-binding factor 3 | chr4:16296008-16297971 FORWARD LENGTH=454)

HSP 1 Score: 50.447 bits (119), Expect = 4.566e-7
Identity = 24/44 (54.55%), Postives = 34/44 (77.27%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLE 134
            RSRARKQAYT+ELEAE+ QL+E N +L++   E+  +++ Q LE
Sbjct:  388 RSRARKQAYTMELEAEIAQLKELNEELQKKQVEIMEKQKNQLLE 431
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT4G34000.1 (| Symbols: ABF3, DPBF5 | abscisic acid responsive elements-binding factor 3 | chr4:16296008-16297971 FORWARD LENGTH=454)

HSP 1 Score: 50.447 bits (119), Expect = 4.566e-7
Identity = 24/44 (54.55%), Postives = 34/44 (77.27%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLE 134
            RSRARKQAYT+ELEAE+ QL+E N +L++   E+  +++ Q LE
Sbjct:  388 RSRARKQAYTMELEAEIAQLKELNEELQKKQVEIMEKQKNQLLE 431
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT3G19290.1 (| Symbols: ABF4, AREB2 | ABRE binding factor 4 | chr3:6687956-6689784 FORWARD LENGTH=431)

HSP 1 Score: 50.447 bits (119), Expect = 4.566e-7
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRG 191
            RSRARKQAYT+ELEAE+ +L++ N +L++  AE+   ++ +  E +     +K Q  R  L G
Sbjct:  367 RSRARKQAYTLELEAEIEKLKKTNQELQKKQAEMVEMQKNELKETSKRPWGSKRQCLRRTLTG 429
[Close]
BLAST of FG532534 vs. TAIR peptide
Match: AT1G45249.1 (| Symbols: ABF2, AREB1, ATAREB1 | abscisic acid responsive elements-binding factor 2 | chr1:17165420-17167415 REVERSE LENGTH=416)

HSP 1 Score: 49.6766 bits (117), Expect = 7.788e-7
Identity = 28/63 (44.44%), Postives = 38/63 (60.32%), Query Frame = 3 
Query:    3 RSRARKQAYTVELEAELNQLREENSQLKQALAELEARRRQQCLEETNATVQTKAQKAREKLRG 191
            RSRARKQAYTVELEAE+ +L+EEN +L++  A +   ++ Q  E  N       +K R    G
Sbjct:  352 RSRARKQAYTVELEAEVAKLKEENDELQRKQARIMEMQKNQETEMRNLLQGGPKKKLRRTESG 414
The following BLAST results are available for this feature:
BLAST of FG532534 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7TJ61_VITVI1.848e-1866.67Whole genome shotgun sequence of line PN40024, sca... [more]
B9HBJ5_POPTR2.414e-1868.06Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
O23964_HELAN1.565e-1768.57Dc3 promoter-binding factor-1 OS=Helianthus annuus... [more]
D7LIQ2_ARALY1.014e-1665.28Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9HQ00_POPTR1.676e-1152.17Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B3U2B5_CUCSA2.858e-1172.00Abscisic acid insensitive OS=Cucumis sativus PE=3 ... [more]
D7LNS0_ARALY1.201e-952.17Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9SRW9_RICCO1.201e-978.05DNA binding protein, putative OS=Ricinus communis ... [more]
B9SCS5_RICCO3.494e-950.72Putative uncharacterized protein OS=Ricinus commun... [more]
A5B2U1_VITVI2.264e-876.74Putative uncharacterized protein OS=Vitis vinifera... [more]
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BLAST of FG532534 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
ABI5_ARATH4.334e-1865.28Protein ABSCISIC ACID-INSENSITIVE 5 OS=Arabidopsis... [more]
AI5L1_ARATH8.754e-1150.72ABSCISIC ACID-INSENSITIVE 5-like protein 1 OS=Arab... [more]
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BLAST of FG532534 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7
Match NameE-valueIdentityDescription
AT2G36270.15.200e-1965.28| Symbols: ABI5, GIA1 | Basic-leucine zipper (bZIP... [more]
AT3G44460.11.050e-1150.72| Symbols: DPBF2, AtbZIP67 | Basic-leucine zipper ... [more]
AT3G19290.33.496e-743.75| Symbols: ABF4 | ABRE binding factor 4 | chr3:668... [more]
AT4G34000.24.566e-754.55| Symbols: ABF3, DPBF5 | abscisic acid responsive ... [more]
AT4G34000.14.566e-754.55| Symbols: ABF3, DPBF5 | abscisic acid responsive ... [more]
AT3G19290.14.566e-741.27| Symbols: ABF4, AREB2 | ABRE binding factor 4 | c... [more]
AT1G45249.17.788e-744.44| Symbols: ABF2, AREB1, ATAREB1 | abscisic acid re... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 52..67
score
NoneNo IPR availableGENE3D1.20.5.170no descriptioncoord: 1..37
score: 5.6
NoneNo IPR availableSEGsegsegcoord: 10..35
score: NAcoord: 64..76
score
IPR001581Leukemia inhibitory factor /oncostatinPFAMPF01291LIF_OSMcoord: 16..71
score: 7
IPR011616bZIP transcription factor, bZIP-1PFAMPF00170bZIP_1coord: 1..36
score: 7.7

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG532534 ID=FG532534; Name=FG532534; organism=Pisum sativum; type=EST; length=443bp
CAAGATCTAGAGCCAGAAAACAGGCATATACAGTTGAATTAGAAGCAGAA
CTGAACCAGTTAAGAGAAGAGAATTCTCAACTGAAACAAGCACTGGCCGA
ACTCGAGGCGAGAAGAAGACAGCAGTGTTTAGAGGAAACAAACGCGACGG
TTCAAACCAAAGCTCAGAAAGCAAGAGAGAAACTGAGAGGCTTAAGAAGG
AACACAAGCTGTCATCTGTGAAGAAATCATCACATGTTGCATGAGTGGAT
CTTCTTGTTTAGTTTAACTGAGAAAATGGCGCATGATGCAAATCAAGAAC
CAAGAAAGTTAAAATAAAAGAGACAGAAACATCTAATTTGGATGGTGATG
TGAAGTTATTCAGTGAACAAATTTTGCACTGTTTGCAAGGATTGAATCAT
CTAATTTGGATGACTCTTTTCAACTGATATATAACTAGTTACT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005125cytokine activity
GO:0003700DNA-binding transcription factor activity
GO:0043565sequence-specific DNA binding
GO:0046983protein dimerization activity
Vocabulary: Cellular Component
TermDefinition
GO:0005576extracellular region
Vocabulary: Biological Process
TermDefinition
GO:0006955immune response
Vocabulary: INTERPRO
TermDefinition
IPR001581Leukemia_IF/oncostatin
IPR011616bZIP transcription factor, bZIP-1