FG534460

EST Overview


Name	FG534460
Unique Name	FG534460
Type	EST
Organism	Pisum sativum (pea)
Sequence length	585

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG534460 vs. TrEMBL
Match: B9HRQ6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_557279 PE=4 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 8.274e-87
Identity = 155/167 (92.81%), Postives = 161/167 (96.41%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            IISSAGYAVASLLLQPKDRHNGNLLFDN GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+QF+RLCVKGYLAARR+M+GII TV LMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFM  VC DAYNKWTTAGYDLIQY+QQGIEK
Sbjct: 1851 IISSAGYAVASLLLQPKDRHNGNLLFDNLGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWSQFVRLCVKGYLAARRYMDGIINTVMLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYIQQGIEK 2017

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BLAST of FG534460 vs. TrEMBL
Match: B9H0N9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_556445 PE=4 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 2.038e-85
Identity = 153/167 (91.62%), Postives = 159/167 (95.21%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            IISSAGYAVASLLLQPKDRHNGNLLFDN GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW QF+ LCVKGYLAARR+M+GII TV LMLDSGLPCFSRGDPIGNLR+RFHPEMSEREAANFM  VC DAYNKWTTAGYDLIQY+QQGIEK
Sbjct: 1857 IISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWLQFVSLCVKGYLAARRYMDGIINTVMLMLDSGLPCFSRGDPIGNLRRRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYIQQGIEK 2023

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BLAST of FG534460 vs. TrEMBL
Match: Q9SXA1_ARATH (Putative Phosphatidylinositol 4-kinase PI4K OS=Arabidopsis thaliana GN=F13F21.23 PE=4 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 5.929e-85
Identity = 151/167 (90.42%), Postives = 160/167 (95.81%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR M+GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FM HVC DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1885 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDLIQYLQQGIEK 2051

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BLAST of FG534460 vs. TrEMBL
Match: O81129_ARATH (Phosphatidylinositol 4-kinase OS=Arabidopsis thaliana GN=At1g49340 PE=2 SV=2)

HSP 1 Score: 317.005 bits (811), Expect = 5.929e-85
Identity = 151/167 (90.42%), Postives = 160/167 (95.81%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR M+GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FM HVC DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1862 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDLIQYLQQGIEK 2028

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BLAST of FG534460 vs. TrEMBL
Match: D7KE30_ARALY (Phosphatidylinositol 4-kinase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474074 PE=4 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 5.929e-85
Identity = 151/167 (90.42%), Postives = 160/167 (95.81%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR M+GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FM HVC DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1852 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDLIQYLQQGIEK 2018

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BLAST of FG534460 vs. TrEMBL
Match: B9RZB3_RICCO (Phosphatidylinositol 4-kinase, putative OS=Ricinus communis GN=RCOM_0937410 PE=4 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 7.744e-85
Identity = 152/166 (91.57%), Postives = 158/166 (95.18%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIE 500
            IISSAGYAVASLLLQPKDRHNGNLLFDN GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSG+MKS+TW QF+ LCVKGYLAARR+M+GII TV LMLDSGLPCFSRGDPIGNLRKRFHPEMS+REAANFM  VC DAYNKWTTAGYDLIQYLQQGIE
Sbjct: 1828 IISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGIMKSETWFQFVSLCVKGYLAARRYMDGIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIE 1993

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BLAST of FG534460 vs. TrEMBL
Match: C5WN42_SORBI (Putative uncharacterized protein Sb01g010410 OS=Sorghum bicolor GN=Sb01g010410 PE=4 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 2.943e-84
Identity = 150/167 (89.82%), Postives = 157/167 (94.01%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILE SPGGNM FESAHFKLSHEMTQLLDPSG MKSDTWNQFLRLCVKGYLAARRHM GI++TV+LM+DSGLPCFSRGDPI NLRKRFHPEM+EREAANFM   C DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1781 MISSAGYAVASLLLQPKDRHNGNLLFDSHGRLVHIDFGFILEISPGGNMGFESAHFKLSHEMTQLLDPSGTMKSDTWNQFLRLCVKGYLAARRHMNGILSTVNLMIDSGLPCFSRGDPINNLRKRFHPEMNEREAANFMIRTCADAYNKWTTAGYDLIQYLQQGIEK 1947

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BLAST of FG534460 vs. TrEMBL
Match: Q8L5D9_ORYSA (Phosphatidylinositol 4-kinase OS=Oryza sativa GN=pi4K1 PE=2 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.556e-84
Identity = 150/167 (89.82%), Postives = 156/167 (93.41%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILE SPGGNM FESAHFKLSHEMTQLLDPSG MKSDTWNQFLRLCVKGYLA RRHM GIITTV+LM+DSGLPCFSRG+PI NLRKRFHPEM+EREAANFM   C DAYNKWTTAGYDLIQYLQQGIEK
Sbjct:  442 MISSAGYAVASLLLQPKDRHNGNLLFDSHGRLVHIDFGFILEISPGGNMGFESAHFKLSHEMTQLLDPSGTMKSDTWNQFLRLCVKGYLAGRRHMNGIITTVNLMVDSGLPCFSRGEPIANLRKRFHPEMNEREAANFMVRTCVDAYNKWTTAGYDLIQYLQQGIEK 608

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BLAST of FG534460 vs. TrEMBL
Match: Q53RI7_ORYSJ (Phosphatidylinositol 3-and 4-kinase, putative OS=Oryza sativa subsp. japonica PE=4 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.556e-84
Identity = 150/167 (89.82%), Postives = 156/167 (93.41%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILE SPGGNM FESAHFKLSHEMTQLLDPSG MKSDTWNQFLRLCVKGYLA RRHM GIITTV+LM+DSGLPCFSRG+PI NLRKRFHPEM+EREAANFM   C DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1784 MISSAGYAVASLLLQPKDRHNGNLLFDSHGRLVHIDFGFILEISPGGNMGFESAHFKLSHEMTQLLDPSGTMKSDTWNQFLRLCVKGYLAGRRHMNGIITTVNLMVDSGLPCFSRGEPIANLRKRFHPEMNEREAANFMVRTCVDAYNKWTTAGYDLIQYLQQGIEK 1950

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BLAST of FG534460 vs. TrEMBL
Match: Q10E17_ORYSJ (Os03g0711200 protein OS=Oryza sativa subsp. japonica GN=Os03g0711200 PE=4 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 6.556e-84
Identity = 150/167 (89.82%), Postives = 156/167 (93.41%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILE SPGGNM FESAHFKLSHEMTQLLDPSG MKSDTWNQFLRLCVKGYLA RRHM GIITTV+LM+DSGLPCFSRG+PI NLRKRFHPEM+EREAANFM   C DAYNKWTTAGYDLIQYLQQGIEK
Sbjct:  442 MISSAGYAVASLLLQPKDRHNGNLLFDSHGRLVHIDFGFILEISPGGNMGFESAHFKLSHEMTQLLDPSGTMKSDTWNQFLRLCVKGYLAGRRHMNGIITTVNLMVDSGLPCFSRGEPIANLRKRFHPEMNEREAANFMVRTCVDAYNKWTTAGYDLIQYLQQGIEK 608

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BLAST of FG534460 vs. SwissProt
Match: PI4KA_BOVIN (Phosphatidylinositol 4-kinase alpha OS=Bos taurus GN=PI4KA PE=2 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 8.269e-37
Identity = 78/165 (47.27%), Postives = 111/165 (67.27%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            I S A Y++   LLQ KDRHNGN++ D  G L+HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R +M+ +++ V+LMLD+GLPCF RG  I  L+ RF P M+EREAANF+  V ++ +    +  YD+IQY Q  I
Sbjct: 1881 IRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHLIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCF-RGQTIKLLKHRFSPNMTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2041

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BLAST of FG534460 vs. SwissProt
Match: PI4KA_HUMAN (Phosphatidylinositol 4-kinase alpha OS=Homo sapiens GN=PI4KA PE=1 SV=3)

HSP 1 Score: 152.14 bits (383), Expect = 1.842e-36
Identity = 77/165 (46.67%), Postives = 110/165 (66.67%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            I S A Y++   LLQ KDRHNGN++ D  G ++HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R +M+ +++ V+LMLD+GLPCF RG  I  L+ RF P M+EREAANF+  V +  +    +  YD+IQY Q  I
Sbjct: 1882 IRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCF-RGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDI 2042

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BLAST of FG534460 vs. SwissProt
Match: STT4_YEAST (Phosphatidylinositol 4-kinase STT4 OS=Saccharomyces cerevisiae GN=STT4 PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.474e-35
Identity = 78/163 (47.85%), Postives = 105/163 (64.42%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY+V S LLQ KDRHNGN+++D+ G  +HIDFGFI +  PGG ++FE+  FKL+ EM +++   G  ++  +  F  LC+K YLAAR H+E II  V+ ML SGLPCF     I NLR RF P+ ++ EAA +M  + + +Y    T GYD  Q L  GI
Sbjct: 1739 SLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGG-IKFEAVPFKLTKEMVKVM--GGSPQTPAYLDFEELCIKAYLAARPHVEAIIECVNPMLGSGLPCFKGHKTIRNLRARFQPQKTDHEAALYMKALIRKSYESIFTKGYDEFQRLTNGI 1898

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BLAST of FG534460 vs. SwissProt
Match: STT4_SCHPO (Phosphatidylinositol 4-kinase stt4 OS=Schizosaccharomyces pombe GN=stt4 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.156e-30
Identity = 73/163 (44.79%), Postives = 99/163 (60.74%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S A Y+V + LLQ KDRHNGN++ D+ G ++HIDFGFI + +PGG + FESA FKL+ EM  ++   G  KS  +  F  LCVK +LA R +   I   V +MLDSGLPCF     I +  +RF   ++ER+A+ FM H+ + +Y    T  YD  Q    GI
Sbjct: 1716 SMAAYSVITYLLQFKDRHNGNIMIDDQGHILHIDFGFIFDIAPGG-ITFESAPFKLTTEMIAVM--GGSNKSQPFQWFQELCVKAFLACRPYAHYICQAVEVMLDSGLPCFKGQLTITHCLERFALNLNERQASTFMLHLIEQSYANKRTLMYDQFQKATNGI 1875

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BLAST of FG534460 vs. SwissProt
Match: PI4P2_HUMAN (Putative phosphatidylinositol 4-kinase alpha-like protein P2 OS=Homo sapiens GN=PI4KAP2 PE=5 SV=2)

HSP 1 Score: 130.568 bits (327), Expect = 5.740e-30
Identity = 71/168 (42.26%), Postives = 107/168 (63.69%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKG-YLAAR--RHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            I S A Y++   LLQ KDRHNGN++ D  G ++HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+  +L      +M+ +++ V++MLD+GLPCF RG  I  L+ RF P M+EREAANF+  V +  +    +  Y++IQY Q  I
Sbjct:  427 IRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVQATWLCGEPGPYMDVVVSLVTIMLDTGLPCF-RGQTIKFLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYNMIQYYQNDI 590

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BLAST of FG534460 vs. SwissProt
Match: PI4KB_RAT (Phosphatidylinositol 4-kinase beta OS=Rattus norvegicus GN=Pi4kb PE=1 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 4.859e-29
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLD-SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY +   LLQ KDRHNGN+L D  G ++HIDFGFIL +SP  N+ FE++ FKL+   T+ +D  G +  D +N +  L ++G +AAR+HM+ ++  V +M   S LPCF     I NL++RFH  M+E +    +  +   +    TT  YD  QYL  GI
Sbjct:  656 SCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLT---TEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815

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BLAST of FG534460 vs. SwissProt
Match: PI4KB_MOUSE (Phosphatidylinositol 4-kinase beta OS=Mus musculus GN=Pi4kb PE=1 SV=2)

HSP 1 Score: 127.487 bits (319), Expect = 4.859e-29
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLD-SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY +   LLQ KDRHNGN+L D  G ++HIDFGFIL +SP  N+ FE++ FKL+   T+ +D  G +  D +N +  L ++G +AAR+HM+ ++  V +M   S LPCF     I NL++RFH  M+E +    +  +   +    TT  YD  QYL  GI
Sbjct:  656 SCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLT---TEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815

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BLAST of FG534460 vs. SwissProt
Match: PI4KB_SORAR (Phosphatidylinositol 4-kinase beta OS=Sorex araneus GN=PI4KB PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 6.346e-29
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLD-SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY +   LLQ KDRHNGN+L D  G ++HIDFGFIL +SP  N+ FE++ FKL+   T+ +D  G +  D +N +  L ++G +AAR+HM+ ++  V +M   S LPCF     I NL++RFH  M+E +    +  +   +    TT  YD  QYL  GI
Sbjct:  641 SCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLT---TEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMNMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 800

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BLAST of FG534460 vs. SwissProt
Match: PI4KB_RHIFE (Phosphatidylinositol 4-kinase beta OS=Rhinolophus ferrumequinum GN=PI4KB PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 6.346e-29
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLD-SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY +   LLQ KDRHNGN+L D  G ++HIDFGFIL +SP  N+ FE++ FKL+   T+ +D  G +  D +N +  L ++G +AAR+HM+ ++  V +M   S LPCF     I NL++RFH  M+E +    +  +   +    TT  YD  QYL  GI
Sbjct:  656 SCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLT---TEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815

[Close]

BLAST of FG534460 vs. SwissProt
Match: PI4KB_PAPAN (Phosphatidylinositol 4-kinase beta OS=Papio anubis GN=PI4KB PE=3 SV=2)

HSP 1 Score: 127.102 bits (318), Expect = 6.346e-29
Identity = 69/164 (42.07%), Postives = 98/164 (59.76%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLD-SGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY +   LLQ KDRHNGN+L D  G ++HIDFGFIL +SP  N+ FE++ FKL+   T+ +D  G +  D +N +  L ++G +AAR+HM+ ++  V +M   S LPCF     I NL++RFH  M+E +    +  +   +    TT  YD  QYL  GI
Sbjct:  656 SCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLT---TEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT1G49340.1 (| Symbols: ATPI4K ALPHA | Phosphatidylinositol 3- and 4-kinase family protein | chr1:18252355-18263967 FORWARD LENGTH=2028)

HSP 1 Score: 317.005 bits (811), Expect = 4.566e-87
Identity = 151/167 (90.42%), Postives = 160/167 (95.81%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR M+GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FM HVC DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1862 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDLIQYLQQGIEK 2028

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT1G49340.2 (| Symbols: ATPI4K ALPHA | Phosphatidylinositol 3- and 4-kinase family protein | chr1:18252355-18263967 FORWARD LENGTH=2028)

HSP 1 Score: 317.005 bits (811), Expect = 4.566e-87
Identity = 151/167 (90.42%), Postives = 160/167 (95.81%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR M+GII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FM HVC DAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1862 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDLIQYLQQGIEK 2028

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT1G51040.1 (| Symbols: | Protein kinase superfamily protein | chr1:18920658-18923314 FORWARD LENGTH=525)

HSP 1 Score: 308.916 bits (790), Expect = 1.243e-84
Identity = 147/167 (88.02%), Postives = 159/167 (95.21%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGIEK 503
            +ISSAGYAVASLLLQPKDRHNGNLLFD+ GRLVHIDFGFILETSPGGNMRFE+AHFKLSHEMTQLLDPSGVMKS TW+QF+ LCVKGYLAARR+M+ II+TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA FM +VC DAYNKWTTAGYDLIQYLQQG+EK
Sbjct:  359 LISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGGNMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT5G09350.1 (| Symbols: PI-4KBETA2, PI4KBETA2 | phosphatidylinositol 4-OH kinase beta2 | chr5:2899365-2905610 REVERSE LENGTH=1116)

HSP 1 Score: 143.665 bits (361), Expect = 6.918e-35
Identity = 73/165 (44.24%), Postives = 103/165 (62.42%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGV-MKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY++   LLQ KDRHNGNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D     + S+ ++ F  LC++G+L  R+H E II  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +  +   + + W T  YD  Q +  GI
Sbjct:  952 SMAGYSLVCYLLQIKDRHNGNLLMDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDAEGLPSEFFDYFKVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGI 1115

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT5G64070.1 (| Symbols: PI-4KBETA1, PI4KBETA1 | phosphatidylinositol 4-OH kinase beta1 | chr5:25637492-25643902 REVERSE LENGTH=1121)

HSP 1 Score: 143.28 bits (360), Expect = 9.035e-35
Identity = 73/165 (44.24%), Postives = 104/165 (63.03%), Query Frame = 3

Query:    9 SSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLD-PSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFMTHVCKDAYNKWTTAGYDLIQYLQQGI 497
            S AGY++   LLQ KDRHNGNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D  +  + S+ ++ F  LC++G+L  R+H E II  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +  +   + + W T  YD  Q +  GI
Sbjct:  957 SMAGYSLVCYLLQVKDRHNGNLLLDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDADGVPSEFFDYFKVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGI 1120

[Close]

BLAST of FG534460 vs. TAIR peptide
Match: AT1G60490.1 (| Symbols: ATVPS34, VPS34, PI3K | vacuolar protein sorting 34 | chr1:22285792-22290190 REVERSE LENGTH=814)

HSP 1 Score: 59.3066 bits (142), Expect = 1.716e-9
Identity = 45/149 (30.20%), Postives = 70/149 (46.98%), Query Frame = 3

Query:    3 IISSAGYAVASLLLQPKDRHNGNLLFDNAGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWNQFLRLCVKGYLAARRHMEGIITTVSLMLDSGLPCFSRGDP---IGNLRKRFHPEMSEREAANFMTHVCKDA 440
            I S AGY+V + +L   DRH  NLL  + GRL H+DF FIL   P    +      KL  EM + +   G  +S  + +F   C + Y   R+    I+    LM  S +P  +  DP   I  L+++F  +M +    +F   +  ++
Sbjct:  654 IKSCAGYSVITYILGIGDRHLDNLLLTDDGRLFHVDFAFILGRDP----KPFPPPMKLCKEMVEAM---GGAESQYYTRFKSYCCEAYNILRKSSNLILNLFHLMAGSTIPDIA-SDPEKGILKLQEKFRLDMDDEACIHFFQDLINES 794

The following BLAST results are available for this feature:

BLAST of FG534460 vs. TrEMBL
BLAST of FG534460 vs. SwissProt
BLAST of FG534460 vs. TAIR peptide

BLAST of FG534460 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HRQ6_POPTR	8.274e-87	92.81	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H0N9_POPTR	2.038e-85	91.62	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SXA1_ARATH	5.929e-85	90.42	Putative Phosphatidylinositol 4-kinase PI4K OS=Ara...	[more]
O81129_ARATH	5.929e-85	90.42	Phosphatidylinositol 4-kinase OS=Arabidopsis thali...	[more]
D7KE30_ARALY	5.929e-85	90.42	Phosphatidylinositol 4-kinase OS=Arabidopsis lyrat...	[more]
B9RZB3_RICCO	7.744e-85	91.57	Phosphatidylinositol 4-kinase, putative OS=Ricinus...	[more]
C5WN42_SORBI	2.943e-84	89.82	Putative uncharacterized protein Sb01g010410 OS=So...	[more]
Q8L5D9_ORYSA	6.556e-84	89.82	Phosphatidylinositol 4-kinase OS=Oryza sativa GN=p...	[more]
Q53RI7_ORYSJ	6.556e-84	89.82	Phosphatidylinositol 3-and 4-kinase, putative OS=O...	[more]
Q10E17_ORYSJ	6.556e-84	89.82	Os03g0711200 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of FG534460 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PI4KA_BOVIN	8.269e-37	47.27	Phosphatidylinositol 4-kinase alpha OS=Bos taurus ...	[more]
PI4KA_HUMAN	1.842e-36	46.67	Phosphatidylinositol 4-kinase alpha OS=Homo sapien...	[more]
STT4_YEAST	3.474e-35	47.85	Phosphatidylinositol 4-kinase STT4 OS=Saccharomyce...	[more]
STT4_SCHPO	1.156e-30	44.79	Phosphatidylinositol 4-kinase stt4 OS=Schizosaccha...	[more]
PI4P2_HUMAN	5.740e-30	42.26	Putative phosphatidylinositol 4-kinase alpha-like ...	[more]
PI4KB_RAT	4.859e-29	42.07	Phosphatidylinositol 4-kinase beta OS=Rattus norve...	[more]
PI4KB_MOUSE	4.859e-29	42.07	Phosphatidylinositol 4-kinase beta OS=Mus musculus...	[more]
PI4KB_SORAR	6.346e-29	42.07	Phosphatidylinositol 4-kinase beta OS=Sorex araneu...	[more]
PI4KB_RHIFE	6.346e-29	42.07	Phosphatidylinositol 4-kinase beta OS=Rhinolophus ...	[more]
PI4KB_PAPAN	6.346e-29	42.07	Phosphatidylinositol 4-kinase beta OS=Papio anubis...	[more]

BLAST of FG534460 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT1G49340.1	4.566e-87	90.42	\| Symbols: ATPI4K ALPHA \| Phosphatidylinositol 3- ...	[more]
AT1G49340.2	4.566e-87	90.42	\| Symbols: ATPI4K ALPHA \| Phosphatidylinositol 3- ...	[more]
AT1G51040.1	1.243e-84	88.02	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT5G09350.1	6.918e-35	44.24	\| Symbols: PI-4KBETA2, PI4KBETA2 \| phosphatidylino...	[more]
AT5G64070.1	9.035e-35	44.24	\| Symbols: PI-4KBETA1, PI4KBETA1 \| phosphatidylino...	[more]
AT1G60490.1	1.716e-9	30.20	\| Symbols: ATVPS34, VPS34, PI3K \| vacuolar protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic	GENE3D	1.10.1070.11	no description	coord: 3..153 score: 9.1
IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic	PFAM	PF00454	PI3_PI4_kinase	coord: 3..114 score: 1.2
IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic	SMART	SM00146	no description	coord: 1..164 score: 8.2
IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic	PROFILE	PS50290	PI3_4_KINASE_3	coord: 1..148 score: 31
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..166 score: 3.1
IPR015433	Phosphatidylinositol Kinase	PANTHER	PTHR10048	PHOSPHATIDYLINOSITOL KINASE	coord: 1..166 score: 5.6e
None	No IPR available	PANTHER	PTHR10048:SF15	PHOSPHATIDYLINOSITOL 4-KINASE	coord: 1..166 score: 5.6e

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG534460 ID=FG534460; Name=FG534460; organism=Pisum sativum; type=EST; length=585bp
AAATCATTAGCAGTGCTGGTTATGCCGTCGCTAGTCTCTTGCTTCAACCA
AAGGATAGACACAATGGGAACCTTCTGTTTGATAATGCTGGGAGGCTTGT
TCATATTGACTTTGGTTTCATTCTAGAAACTTCTCCTGGTGGGAATATGC
GTTTTGAAAGTGCACATTTTAAGCTGAGTCACGAGATGACACAATTACTG
GATCCATCTGGTGTTATGAAGAGTGATACATGGAACCAATTTTTAAGATT
GTGTGTGAAAGGATACCTTGCTGCCCGTCGACATATGGAAGGGATTATCA
CGACTGTGTCATTGATGCTAGACAGTGGGTTACCTTGCTTCAGCAGGGGT
GACCCCATTGGTAATCTTCGCAAGAGATTTCATCCCGAGATGAGTGAGCG
TGAAGCGGCTAATTTCATGACCCATGTATGTAAAGATGCTTACAACAAGT
GGACAACTGCTGGTTATGACTTGATACAGTATCTACAGCAAGGCATCGAA
AAGTAAGAGATAATATAGGACTGACCTAAGGTGTACATGTTTGGTTGTAA
TATTAAATTCATTGCTGTCTTATGTTATGATTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004428	obsolete inositol or phosphatidylinositol kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0046854	phosphatidylinositol phosphate biosynthetic process
GO:0048015	phosphatidylinositol-mediated signaling

Vocabulary: **INTERPRO**
Term	Definition
IPR000403	PI3/4_kinase_cat_dom
IPR011009	Kinase-like_dom_sf
IPR015433	PI_Kinase

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