FG536453

EST Overview


Name	FG536453
Unique Name	FG536453
Type	EST
Organism	Pisum sativum (pea)
Sequence length	526

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG536453 vs. TrEMBL
Match: B9GXB2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_813719 PE=3 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.830e-53
Identity = 101/131 (77.10%), Postives = 118/131 (90.08%), Query Frame = 3

Query:    6 YKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            YKYYP++++LAAN Q+LVVACALTE+TRHI+NREVI+ALGPKGV++NIGRGP +DE ELVSALVEGRLGGAGLDVF++EP+VPEEL  L+NVVL PHV S T ET K+MADLV+ NLEAHF  KPLLTPVL
Sbjct:  184 YKYYPSVVELAANCQILVVACALTEETRHIINREVINALGPKGVLINIGRGPHVDEPELVSALVEGRLGGAGLDVFQDEPNVPEELFGLENVVLLPHVGSGTMETRKEMADLVVGNLEAHFLNKPLLTPVL 314

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BLAST of FG536453 vs. TrEMBL
Match: A9PIN2_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.830e-53
Identity = 101/131 (77.10%), Postives = 118/131 (90.08%), Query Frame = 3

Query:    6 YKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            YKYYP++++LAAN Q+LVVACALTE+TRHI+NREVI+ALGPKGV++NIGRGP +DE ELVSALVEGRLGGAGLDVF++EP+VPEEL  L+NVVL PHV S T ET K+MADLV+ NLEAHF  KPLLTPVL
Sbjct:  184 YKYYPSVVELAANCQILVVACALTEETRHIINREVINALGPKGVLINIGRGPHVDEPELVSALVEGRLGGAGLDVFQDEPNVPEELFGLENVVLLPHVGSGTMETRKEMADLVVGNLEAHFLNKPLLTPVL 314

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BLAST of FG536453 vs. TrEMBL
Match: D7U0H9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017069001 PE=4 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 9.944e-53
Identity = 101/132 (76.52%), Postives = 119/132 (90.15%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YKYYP++++LA+N Q+LVVACALT +TRHI+NREVI+ALGPKGV++NIGRG  +DE ELVSALVEGRLGGAGLDVFENEP+VPEELL++DNVVL PHV S T ET KDMADLV+ NLEAHF  KPLLTPV+
Sbjct:  115 NYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLTPVV 246

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BLAST of FG536453 vs. TrEMBL
Match: A5CAL1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025327 PE=3 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 9.944e-53
Identity = 101/132 (76.52%), Postives = 119/132 (90.15%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YKYYP++++LA+N Q+LVVACALT +TRHI+NREVI+ALGPKGV++NIGRG  +DE ELVSALVEGRLGGAGLDVFENEP+VPEELL++DNVVL PHV S T ET KDMADLV+ NLEAHF  KPLLTPV+
Sbjct:  182 NYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLTPVV 313

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BLAST of FG536453 vs. TrEMBL
Match: B9GG33_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_548856 PE=3 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.875e-51
Identity = 99/132 (75.00%), Postives = 116/132 (87.88%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YK+Y NI+DLA + Q+L+VACALTE+TRHI+NREVIDALGPKG+++NIGRG  +DE ELVSAL+EGRLGGAGLDV+ENEPDVPEELL L NVVL PHV SDT ET   MADLVI+NL+AHFS K LLTPV+
Sbjct:  184 NYKFYSNIIDLATSCQILIVACALTEETRHIINREVIDALGPKGILINIGRGAHVDETELVSALLEGRLGGAGLDVYENEPDVPEELLGLGNVVLQPHVGSDTVETSDAMADLVISNLKAHFSKKSLLTPVI 315

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BLAST of FG536453 vs. TrEMBL
Match: B9RQK9_RICCO (Glycerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1493920 PE=3 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 4.178e-51
Identity = 99/131 (75.57%), Postives = 116/131 (88.55%), Query Frame = 3

Query:    6 YKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            YKYYP++++LAAN Q+LVVACALTE+T HIVNREVI+ALGPKGV++NIGRGP +DE ELVSAL+EGRLGGAGLDVFE+EP+VPE+L SL+NVVL PHV S T ET   MADLV+ NLEAHF  KPLLTPV+
Sbjct:  184 YKYYPSVVELAANCQILVVACALTEETHHIVNREVINALGPKGVLINIGRGPHVDEPELVSALLEGRLGGAGLDVFEDEPNVPEQLFSLENVVLLPHVGSGTVETRTAMADLVVGNLEAHFLNKPLLTPVV 314

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BLAST of FG536453 vs. TrEMBL
Match: Q65CJ7_SOLSC (Hydroxyphenylpyruvate reductase (HPPR) OS=Solenostemon scutellarioides GN=hppr PE=1 SV=2)

HSP 1 Score: 200.29 bits (508), Expect = 6.033e-50
Identity = 97/132 (73.48%), Postives = 114/132 (86.36%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +Y YY ++++LA+NS +LVVAC LT +T HI+NREVIDALGPKGV++NIGRGP +DE ELVSALVEGRLGGAGLDVFE EP+VPE+L  L+NVVL PHV S T ET K MADLV+ NLEAHFSGKPLLTPV+
Sbjct:  182 NYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNLEAHFSGKPLLTPVV 313

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BLAST of FG536453 vs. TrEMBL
Match: B9RQL1_RICCO (Glycerate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1493940 PE=3 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 6.033e-50
Identity = 97/132 (73.48%), Postives = 112/132 (84.85%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YKY+ NILDLA   Q+LVVACALTE+TRHI+NREVIDALGPKG+++NIGRG  +DE ELVSAL+EGRL GAG DV+ENEP VPE+L SLDNV L PH+ SDT ET   MADLVIANLEAH + KPLLTPV+
Sbjct:  182 NYKYFSNILDLAKTCQILVVACALTEETRHIINREVIDALGPKGILINIGRGAHVDEPELVSALLEGRLAGAGFDVYENEPMVPEQLFSLDNVFLQPHIGSDTVETSNAMADLVIANLEAHLTNKPLLTPVI 313

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BLAST of FG536453 vs. TrEMBL
Match: Q15KG6_SALMI (Putative hydroxyphenylpyruvate reductase OS=Salvia miltiorrhiza GN=hppr PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 7.879e-50
Identity = 97/132 (73.48%), Postives = 114/132 (86.36%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +Y YY ++++LA+NS +LVVACALT +T HIVNREV+DALGPKGV++NIGRGP +DE ELVSALVEGRLGGAGLDVFE EP+VPE+L  L+NVVL PHV S T ET K MADLV+ NLEAHFS KPLLTPV+
Sbjct:  182 NYTYYGSVVELASNSDILVVACALTPETTHIVNREVMDALGPKGVLINIGRGPHVDEAELVSALVEGRLGGAGLDVFEKEPEVPEQLFGLENVVLLPHVGSGTVETRKVMADLVLGNLEAHFSSKPLLTPVV 313

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BLAST of FG536453 vs. TrEMBL
Match: A9CBF7_SALMI (Putative hydroxyphenylpyruvate reductase OS=Salvia miltiorrhiza PE=2 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 6.670e-49
Identity = 95/132 (71.97%), Postives = 114/132 (86.36%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +Y YY ++++LA+NS +LVVACALT +T HIVNREV+DALGPKGV++NIGRGP +DE ELVSALV+GRLGGAGLDVFE EP+VPE+L  L+NVVL PHV S T ET K +ADLV+ NLEAHFS KPLLTPV+
Sbjct:  182 NYTYYGSVVELASNSDILVVACALTPETTHIVNREVMDALGPKGVLINIGRGPHVDEAELVSALVKGRLGGAGLDVFEKEPEVPEQLFGLENVVLLPHVGSGTVETRKVIADLVLGNLEAHFSSKPLLTPVV 313

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BLAST of FG536453 vs. SwissProt
Match: GYAR_PYRFU (Glyoxylate reductase OS=Pyrococcus furiosus GN=gyaR PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.663e-22
Identity = 54/121 (44.63%), Postives = 77/121 (63.64%), Query Frame = 3

Query:   15 YPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            Y  + ++   S  +++A  LT++T +++N E +  + P  ++VNI RG ++D   L+ AL EG + GAGLDVFE EP   EEL SLDNVVLTPH+ S T E  + MA+LV  NL A   G+
Sbjct:  195 YRPLEEVLKESDFVILAVPLTKETMYMINEERLKLMKPTAILVNIARGKVVDTKALIKALKEGWIAGAGLDVFEEEPYYNEELFSLDNVVLTPHIGSATFEAREAMAELVARNLIAFKRGE 315

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BLAST of FG536453 vs. SwissProt
Match: GYAR_THEON (Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) GN=gyaR PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.322e-21
Identity = 54/116 (46.55%), Postives = 73/116 (62.93%), Query Frame = 3

Query:   30 DLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            +L   S  +V+   LT++T H++N E +  + P  ++VNI RG ++D   LV AL EG + GAGLDVFE EP   EEL SLDNVVL PH+ S T    + MA+LV  NL A  +G+
Sbjct:  200 ELLKESDFVVLVVPLTKETYHMINEERLKLMKPTAILVNIARGKVVDTEALVKALREGWIAGAGLDVFEEEPYYHEELFSLDNVVLAPHIGSATYGAREGMAELVARNLIAFKNGE 315

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BLAST of FG536453 vs. SwissProt
Match: GHRB_ERWT9 (Glyoxylate/hydroxypyruvate reductase B OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=ghrB PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.726e-21
Identity = 55/122 (45.08%), Postives = 75/122 (61.48%), Query Frame = 3

Query:   15 YPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEP-DVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            Y ++  L A S  L ++  LTEQT H++ RE +  +    +++N GRGP++DE  L++AL +G L  AGLDVFE EP  V  ELL+L NVV  PH+ S T ET   MA   + NL A  +GK
Sbjct:  191 YCDLDTLLAESDFLCISLPLTEQTHHLIGREQLAKMKRSAILINAGRGPVVDEQALIAALKDGTLHAAGLDVFEQEPLPVSSELLALRNVVALPHIGSATHETRYGMAKDAVDNLIAALNGK 312

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BLAST of FG536453 vs. SwissProt
Match: GYAR_PYRHO (Glyoxylate reductase OS=Pyrococcus horikoshii GN=gyaR PE=1 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 6.559e-21
Identity = 53/116 (45.69%), Postives = 71/116 (61.21%), Query Frame = 3

Query:   30 DLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            DL   S  +V+A  LT +T H++N E +  +    +++NI RG ++D   LV AL EG + GAGLDVFE EP   EEL  LDNVVLTPH+ S +    + MA+LV  NL A   G+
Sbjct:  200 DLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAILINIARGKVVDTNALVKALKEGWIAGAGLDVFEEEPYYNEELFKLDNVVLTPHIGSASFGAREGMAELVAKNLIAFKRGE 315

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BLAST of FG536453 vs. SwissProt
Match: GHRB_ENTAG (Glyoxylate/hydroxypyruvate reductase B OS=Enterobacter agglomerans GN=tkrA PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.119e-20
Identity = 53/122 (43.44%), Postives = 74/122 (60.66%), Query Frame = 3

Query:   15 YPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEP-DVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            Y ++  L   S  L ++  LTEQT H++ RE +  + P  +++N GRGP++DE  L++AL +  +  AGLDVFE EP  V  ELL+L NVV  PH+ S T ET   MA   + NL A  +GK
Sbjct:  190 YCDLDTLLRESDFLCISLPLTEQTHHMIGREQLAKMKPSAILINAGRGPVVDEQALIAALKDKTIHAAGLDVFEQEPLPVDSELLTLPNVVALPHIGSATHETRYGMARDAVDNLIAALAGK 311

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BLAST of FG536453 vs. SwissProt
Match: GHRB_PECCP (Glyoxylate/hydroxypyruvate reductase B OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=ghrB PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.461e-20
Identity = 53/116 (45.69%), Postives = 69/116 (59.48%), Query Frame = 3

Query:   33 LAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEP-DVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            L A S  L +   LT +T H++ RE +  + P  +++NIGRG ++DE  L  ALV+G + GAGLDVF  EP  V   LL L NVV  PH+ S T ET  DMA   + NL A  SG+
Sbjct:  193 LLAESDFLCITLPLTTETHHLIGREQLAKMKPSAILINIGRGAVVDEDALTEALVKGTIQGAGLDVFVKEPLPVDSPLLDLPNVVALPHIGSATHETRYDMAACAVDNLIAALSGE 308

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BLAST of FG536453 vs. SwissProt
Match: GYAR_PYRKO (Glyoxylate reductase OS=Pyrococcus kodakaraensis GN=gyaR PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.553e-20
Identity = 51/121 (42.15%), Postives = 76/121 (62.81%), Query Frame = 3

Query:   15 YPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            + ++ DL   S  +V+A  LT++T++++N E +  +    ++VNI RG ++D   L+ AL EG + GAGLDV+E EP   EEL SL NVVL PH+ S T    + MA+LV  NL A  +G+
Sbjct:  195 FRSLEDLLRESDFVVLAVPLTKETQYMINEERLRLMKKTAILVNIARGKVVDTKALMKALKEGWIAGAGLDVYEEEPYYNEELFSLKNVVLAPHIGSATYGAREGMAELVARNLIAFKNGE 315

[Close]

BLAST of FG536453 vs. SwissProt
Match: GYAR_PYRAB (Glyoxylate reductase OS=Pyrococcus abyssi GN=gyaR PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.553e-20
Identity = 51/116 (43.97%), Postives = 71/116 (61.21%), Query Frame = 3

Query:   30 DLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            +L   S  +V+A  L ++T H++N E +  +    +++N+ RG +ID   L+ AL EG + GAGLDV+E EP   EEL SLDNVVLTPH+ S T    + MA LV  NL A   G+
Sbjct:  201 ELLRESDFVVLAVPLNKETYHMINEERLKMMKRTAILINVARGKVIDTKALIKALKEGWIAGAGLDVYEEEPYYNEELFSLDNVVLTPHIGSATFGAREGMAKLVAENLIAFKRGE 316

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BLAST of FG536453 vs. SwissProt
Match: GYAR_THEGJ (Glyoxylate reductase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=gyaR PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.252e-20
Identity = 50/116 (43.10%), Postives = 71/116 (61.21%), Query Frame = 3

Query:   30 DLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            +L   S  +V+A  LT++T H++    +  + P  ++VNI RG ++D   L+ AL EG + GAGLDVFE EP   EEL SL NV+L PH+ S T    + MA+LV  NL A  +G+
Sbjct:  200 ELLKESDFVVLAVPLTKETYHMIGERELKLMKPTAILVNIARGKVVDTEALIKALKEGWIAGAGLDVFEEEPYYNEELFSLKNVILAPHIGSATFGAREGMAELVARNLIAFKNGE 315

[Close]

BLAST of FG536453 vs. SwissProt
Match: GHRB_ERWCT (Glyoxylate/hydroxypyruvate reductase B OS=Erwinia carotovora subsp. atroseptica GN=ghrB PE=3 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 7.252e-20
Identity = 52/116 (44.83%), Postives = 68/116 (58.62%), Query Frame = 3

Query:   33 LAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEP-DVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGK 377
            L A S  L +   LT +T H++ RE +  + P  +++NIGRG ++DE  L  ALV+G +  AGLDVF  EP  V   LL L NVV  PH+ S T ET  DMA   + NL A  SG+
Sbjct:  193 LLAESDFLCITLPLTAETHHLIGREQLAKMKPSAILINIGRGAVVDEDALTEALVKGTIQAAGLDVFVKEPLPVDSPLLDLPNVVALPHIGSATHETRYDMAACAVDNLIAALSGQ 308

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BLAST of FG536453 vs. TAIR peptide
Match: AT1G79870.2 (| Symbols: | D-isomer specific 2-hydroxyacid dehydrogenase family protein | chr1:30044794-30045851 FORWARD LENGTH=294)

HSP 1 Score: 195.667 bits (496), Expect = 1.263e-50
Identity = 95/132 (71.97%), Postives = 109/132 (82.58%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YKYYP ++DLA NS +LVVAC LTEQTRHIV+R+V+DALG KGV++NIGRGP +DE EL+ AL EGRLGGA LDVFE EP VPEEL  L+NVVL PHV S T ET   MADLV+ NLEAHFSGK LLTPV+
Sbjct:  163 AYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLLTPVV 294

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G79870.1 (| Symbols: | D-isomer specific 2-hydroxyacid dehydrogenase family protein | chr1:30044794-30045851 FORWARD LENGTH=313)

HSP 1 Score: 195.667 bits (496), Expect = 1.263e-50
Identity = 95/132 (71.97%), Postives = 109/132 (82.58%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            +YKYYP ++DLA NS +LVVAC LTEQTRHIV+R+V+DALG KGV++NIGRGP +DE EL+ AL EGRLGGA LDVFE EP VPEEL  L+NVVL PHV S T ET   MADLV+ NLEAHFSGK LLTPV+
Sbjct:  182 AYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLLTPVV 313

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G12550.1 (| Symbols: | D-isomer specific 2-hydroxyacid dehydrogenase family protein | chr1:4274649-4275831 FORWARD LENGTH=323)

HSP 1 Score: 162.54 bits (410), Expect = 1.185e-40
Identity = 76/130 (58.46%), Postives = 102/130 (78.46%), Query Frame = 3

Query:    6 YKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPV 395
            Y+YY +IL LA N+ VLV+ C+LT++T HIVNREV++ LG  GV++N+GRG +IDE E+V  LV+G +GGAGLDVFENEP VP+EL  LDNVVL+PH A  T  +  ++A + +ANL+A FS +PLL+PV
Sbjct:  191 YRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALANLKAFFSNRPLLSPV 320

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT2G45630.2 (| Symbols: | D-isomer specific 2-hydroxyacid dehydrogenase family protein | chr2:18796000-18797089 FORWARD LENGTH=338)

HSP 1 Score: 153.68 bits (387), Expect = 5.504e-38
Identity = 73/131 (55.73%), Postives = 98/131 (74.81%), Query Frame = 3

Query:    6 YKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            Y YY +I ++AANS  L++ C L E+T  ++N++V+ ALG +GVIVN+ RG IIDE E+V  L EG +GGAGLDVFE+EP+VP+EL  LDNVV +PH A  T E  +++  +V+ N+EA FS KPLLTPVL
Sbjct:  208 YHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMTLEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G68010.1 (| Symbols: HPR, ATHPR1 | hydroxypyruvate reductase | chr1:25493418-25495720 FORWARD LENGTH=386)

HSP 1 Score: 75.8702 bits (185), Expect = 1.459e-14
Identity = 40/127 (31.50%), Postives = 69/127 (54.33%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPL 383
            ++K   ++ ++   + ++ +   L + T H+VN+E +  +  + ++VN  RGP+IDE  LV  L E  +   GLDVFE EP +   L    N ++ PH+AS ++ T + MA L   N+     G P+
Sbjct:  223 TWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPI 349

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G72190.1 (| Symbols: | D-isomer specific 2-hydroxyacid dehydrogenase family protein | chr1:27167458-27169696 REVERSE LENGTH=373)

HSP 1 Score: 74.3294 bits (181), Expect = 4.244e-14
Identity = 41/111 (36.94%), Postives = 64/111 (57.66%), Query Frame = 3

Query:   15 YPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEP-DVPEELLSLDNVVLTPHVASDTEETCKDMADLV 344
            + +I   A  + ++VV   L ++T  IVN+E I ++    ++VNI RG +I+       L  G LGG G+DV  +EP D  + +L   NV++TPHVA  TE + + MA +V
Sbjct:  243 HEDIYTFAGKADIVVVCLRLNKETAEIVNKEFICSMKKGALLVNIARGGLINYESAFQNLESGHLGGLGIDVAWSEPFDPNDPILKFKNVIITPHVAGVTEYSYRSMAKIV 353

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G68010.2 (| Symbols: HPR | hydroxypyruvate reductase | chr1:25493418-25495720 FORWARD LENGTH=387)

HSP 1 Score: 71.2478 bits (173), Expect = 3.593e-13
Identity = 40/128 (31.25%), Postives = 69/128 (53.91%), Query Frame = 3

Query:    3 SYKYYPNILDLAANSQVLVVACALTEQTRHIVNREVIDALGP-KGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPL 383
            ++K   ++ ++   + ++ +   L + T H+VN+E +  +   + ++VN  RGP+IDE  LV  L E  +   GLDVFE EP +   L    N ++ PH+AS ++ T + MA L   N+     G P+
Sbjct:  223 TWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKVEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPI 350

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT5G28310.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr5:10300117-10301844 REVERSE LENGTH=233)

HSP 1 Score: 67.3958 bits (163), Expect = 5.188e-12
Identity = 39/89 (43.82%), Postives = 50/89 (56.18%), Query Frame = 3

Query:  132 GVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHFSGKPLLTPVL 398
            GVIVN+  G IIDE E+                     +VP+EL  LDNVV +PH A  T E  +++  +V+ N+EA FS KPLLTPVL
Sbjct:  165 GVIVNVALGAIIDEEEM--------------------SNVPKELFELDNVVFSPHCAFMTLEGLEELGKVVVGNIEAFFSNKPLLTPVL 233

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G01510.1 (| Symbols: AN | NAD(P)-binding Rossmann-fold superfamily protein | chr1:187235-189836 FORWARD LENGTH=636)

HSP 1 Score: 59.3066 bits (142), Expect = 1.413e-9
Identity = 33/113 (29.20%), Postives = 57/113 (50.44%), Query Frame = 3

Query:   30 DLAANSQVLVVACALTEQTRHIVNREVIDALGPKGVIVNIGRGPIIDELELVSALVEGRLGGAGLDVFENEPDVPEELLSLDNVVLTPHVASDTEETCKDMADLVIANLEAHF 368
            DL A S V+ + CALT  T  I+N E +  + P   +VN G   ++D+  +   L++G + G  LD  E    +   +  + NV++ P  A  +EE   ++ +  I+ L + F
Sbjct:  219 DLLAASDVISLHCALTNDTVQILNAECLQHIKPGAFLVNTGSCQLLDDCAVKQLLIDGTIAGCALDGAEGPQWMEAWVKEMPNVLILPRSADYSEEVWMEIREKAISILHSFF 331

[Close]

BLAST of FG536453 vs. TAIR peptide
Match: AT1G17745.2 (| Symbols: PGDH | D-3-phosphoglycerate dehydrogenase | chr1:6101157-6104979 FORWARD LENGTH=651)

HSP 1 Score: 58.151 bits (139), Expect = 3.147e-9
Identity = 39/115 (33.91%), Postives = 61/115 (53.04%), Query Frame = 3

Query:   72 LTEQTRHIVNREVIDALGPKGV-IVNIGRGPIIDELELVSALVEGRLGGAGLDVF-ENEPDVPEELLSLDNVVLTPHVASDTEETCK----DMADLVIANLEAHFSGKPLLTPVL 398
            LT  T+ + N E    +  KGV ++N+ RG +IDE  LV AL  G +  A LDVF E  P     L+  +NV +TPH+ + T+E  +    ++A+ V   L+   S   +  P++
Sbjct:  312 LTPATKKVFNDETFSKM-KKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMV 425

The following BLAST results are available for this feature:

BLAST of FG536453 vs. TrEMBL
BLAST of FG536453 vs. SwissProt
BLAST of FG536453 vs. TAIR peptide

BLAST of FG536453 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9GXB2_POPTR	5.830e-53	77.10	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PIN2_9ROSI	5.830e-53	77.10	Putative uncharacterized protein OS=Populus tricho...	[more]
D7U0H9_VITVI	9.944e-53	76.52	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5CAL1_VITVI	9.944e-53	76.52	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9GG33_POPTR	1.875e-51	75.00	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RQK9_RICCO	4.178e-51	75.57	Glycerate dehydrogenase, putative OS=Ricinus commu...	[more]
Q65CJ7_SOLSC	6.033e-50	73.48	Hydroxyphenylpyruvate reductase (HPPR) OS=Solenost...	[more]
B9RQL1_RICCO	6.033e-50	73.48	Glycerate dehydrogenase, putative OS=Ricinus commu...	[more]
Q15KG6_SALMI	7.879e-50	73.48	Putative hydroxyphenylpyruvate reductase OS=Salvia...	[more]
A9CBF7_SALMI	6.670e-49	71.97	Putative hydroxyphenylpyruvate reductase OS=Salvia...	[more]

BLAST of FG536453 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GYAR_PYRFU	2.663e-22	44.63	Glyoxylate reductase OS=Pyrococcus furiosus GN=gya...	[more]
GYAR_THEON	1.322e-21	46.55	Glyoxylate reductase OS=Thermococcus onnurineus (s...	[more]
GHRB_ERWT9	1.726e-21	45.08	Glyoxylate/hydroxypyruvate reductase B OS=Erwinia ...	[more]
GYAR_PYRHO	6.559e-21	45.69	Glyoxylate reductase OS=Pyrococcus horikoshii GN=g...	[more]
GHRB_ENTAG	1.119e-20	43.44	Glyoxylate/hydroxypyruvate reductase B OS=Enteroba...	[more]
GHRB_PECCP	1.461e-20	45.69	Glyoxylate/hydroxypyruvate reductase B OS=Pectobac...	[more]
GYAR_PYRKO	5.553e-20	42.15	Glyoxylate reductase OS=Pyrococcus kodakaraensis G...	[more]
GYAR_PYRAB	5.553e-20	43.97	Glyoxylate reductase OS=Pyrococcus abyssi GN=gyaR ...	[more]
GYAR_THEGJ	7.252e-20	43.10	Glyoxylate reductase OS=Thermococcus gammatolerans...	[more]
GHRB_ERWCT	7.252e-20	44.83	Glyoxylate/hydroxypyruvate reductase B OS=Erwinia ...	[more]

BLAST of FG536453 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G79870.2	1.263e-50	71.97	\| Symbols: \| D-isomer specific 2-hydroxyacid dehy...	[more]
AT1G79870.1	1.263e-50	71.97	\| Symbols: \| D-isomer specific 2-hydroxyacid dehy...	[more]
AT1G12550.1	1.185e-40	58.46	\| Symbols: \| D-isomer specific 2-hydroxyacid dehy...	[more]
AT2G45630.2	5.504e-38	55.73	\| Symbols: \| D-isomer specific 2-hydroxyacid dehy...	[more]
AT1G68010.1	1.459e-14	31.50	\| Symbols: HPR, ATHPR1 \| hydroxypyruvate reductase...	[more]
AT1G72190.1	4.244e-14	36.94	\| Symbols: \| D-isomer specific 2-hydroxyacid dehy...	[more]
AT1G68010.2	3.593e-13	31.25	\| Symbols: HPR \| hydroxypyruvate reductase \| chr1:...	[more]
AT5G28310.1	5.188e-12	43.82	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]
AT1G01510.1	1.413e-9	29.20	\| Symbols: AN \| NAD(P)-binding Rossmann-fold super...	[more]
AT1G17745.2	3.147e-9	33.91	\| Symbols: PGDH \| D-3-phosphoglycerate dehydrogena...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding	PFAM	PF02826	2-Hacid_dh_C	coord: 7..100 score: 1.2
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 3..101 score: 1.5
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE	coord: 2..132 score: 8.4
None	No IPR available	PANTHER	PTHR10996:SF15	GLYCERATE DEHYDROGENASE	coord: 2..132 score: 8.4
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 2..101 score: 1.6
None	No IPR available	SEG	seg	seg	coord: 10..23 score: NAcoord: 38..42 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG536453 ID=FG536453; Name=FG536453; organism=Pisum sativum; type=EST; length=526bp
GATCATACAAGTATTACCCTAACATCCTTGATTTGGCAGCTAACTCTCAA
GTACTTGTTGTCGCGTGTGCCCTAACCGAACAAACGCGACACATTGTGAA
CCGTGAAGTTATTGATGCATTGGGTCCAAAAGGGGTTATTGTCAATATTG
GACGTGGACCGATCATCGATGAACTCGAACTTGTGTCTGCTTTGGTCGAA
GGACGGTTAGGTGGAGCAGGCCTTGATGTGTTTGAGAATGAACCCGATGT
TCCGGAGGAGTTATTGAGCCTTGATAATGTTGTGCTCACTCCTCATGTAG
CAAGTGATACTGAAGAGACTTGCAAAGACATGGCAGATCTTGTGATTGCA
AACTTGGAGGCTCACTTTAGTGGCAAGCCACTTTTGACACCAGTCTTGTG
ATTTCATTGATTGACTTATTATATATTGAATATTGGTGCCAATGTTACAT
AATTTATGGTGTTCTGGACATTTCTTGGCTGATGCTGGACGCACGATTTT
ATTCAAATAAAGCTTGTCTTTCATTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0048037	obsolete cofactor binding
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR006140	D-isomer_DH_NAD-bd
IPR016040	NAD(P)-bd_dom

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