FG538367

EST Overview


Name	FG538367
Unique Name	FG538367
Type	EST
Organism	Pisum sativum (pea)
Sequence length	561

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG538367 vs. TrEMBL
Match: B7FIG7_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.447e-45
Identity = 68/92 (73.91%), Postives = 77/92 (83.70%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRKTSANDGYGNYENHK-VRKTIVS 428
            +K G + G     FIFA+IAN TYVGSILVRTTEWESIKANMPWLLDA VCVALDLFII+QYINYRYHRKT+ +  YGNY+++K  RKTIVS
Sbjct:  289 IKRGSVEGLNPFMFIFALIANATYVGSILVRTTEWESIKANMPWLLDAIVCVALDLFIILQYINYRYHRKTTTSSDYGNYQDYKEARKTIVS 380

HSP 2 Score: 71.633 bits (174), Expect = 2.447e-45
Identity = 35/39 (89.74%), Postives = 37/39 (94.87%), Query Frame = 1

Query:    4 HALKVGYIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            +ALKVGYIAL+GRKLLSQEH T HSALGQWLGWLMAAIY
Sbjct:  240 NALKVGYIALSGRKLLSQEHVT-HSALGQWLGWLMAAIY 277

[Close]

BLAST of FG538367 vs. TrEMBL
Match: C6TMI0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.600e-38
Identity = 49/64 (76.56%), Postives = 55/64 (85.94%), Query Frame = 3

Query:  195 FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRKTSANDGY 386
            F+FA++AN TYVGSILVRTTEWESIKANMPWLLDA +CVALD+FII QYI YRY R+  A D Y
Sbjct:  307 FVFALVANVTYVGSILVRTTEWESIKANMPWLLDAVICVALDIFIISQYIYYRYFRRREAKDDY 370

HSP 2 Score: 51.6026 bits (122), Expect = 3.600e-38
Identity = 25/40 (62.50%), Postives = 28/40 (70.00%), Query Frame = 1

Query:    1 GHALKVGYIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            G+AL+ GYI   GRKLL +     HS  GQWLGWLMAAIY
Sbjct:  245 GNALRQGYIGYGGRKLLQEYEK--HSTFGQWLGWLMAAIY 282

HSP 3 Score: 43.8986 bits (102), Expect = 3.600e-38
Identity = 18/22 (81.82%), Postives = 20/22 (90.91%), Query Frame = 2

Query:  128 GRIPQIWLNIKRGSVEGLNPLI 193
            GR+PQIWLNIKR SVEGLNP +
Sbjct:  285 GRVPQIWLNIKRSSVEGLNPFM 306

[Close]

BLAST of FG538367 vs. TrEMBL
Match: B9T4V4_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0411610 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.333e-29
Identity = 52/82 (63.41%), Postives = 61/82 (74.39%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRKTSANDG--YGNY 395
            +K G + G     FIFA++AN TYV SI+VRTTEWESIKANMPWLLDA VCVALD FII+QY+ YRY R+     G  YG+Y
Sbjct:  291 IKRGSVEGLNPLMFIFALVANLTYVLSIVVRTTEWESIKANMPWLLDAAVCVALDFFIILQYVYYRYFREKKMRHGEDYGDY 372

HSP 2 Score: 50.8322 bits (120), Expect = 1.333e-29
Identity = 23/38 (60.53%), Postives = 26/38 (68.42%), Query Frame = 1

Query:    7 ALKVGYIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            AL   YI +T R+LL +     HSA GQWLGWLMAAIY
Sbjct:  242 ALTDAYIGVTSRRLLHEGSGMEHSAFGQWLGWLMAAIY 279

[Close]

BLAST of FG538367 vs. TrEMBL
Match: D7SPK1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_23.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019034001 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.090e-28
Identity = 53/82 (64.63%), Postives = 62/82 (75.61%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRK--TSANDGYGNY 395
            +K G + G     F+FA+IAN TYV SILVRTTEWE IKANMPWLLDA VCV LDLFII+QYI Y+Y RK  +SA +  G+Y
Sbjct:  291 IKRGSVEGLNPLMFVFALIANVTYVASILVRTTEWEKIKANMPWLLDAAVCVMLDLFIILQYIYYKYLRKKISSAGEDLGDY 372

HSP 2 Score: 43.8986 bits (102), Expect = 9.090e-28
Identity = 21/32 (65.62%), Postives = 23/32 (71.88%), Query Frame = 1

Query:   25 IALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            +  TGRKLL  E    +SA GQWLGWLMAAIY
Sbjct:  249 LGFTGRKLL-HESGMENSAFGQWLGWLMAAIY 279

[Close]

BLAST of FG538367 vs. TrEMBL
Match: Q94AH7_ARATH (Putative uncharacterized protein At4g36850 OS=Arabidopsis thaliana GN=At4g36850 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 4.423e-27
Identity = 54/95 (56.84%), Postives = 67/95 (70.53%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHR----KTSANDGYGNYENHKVRKTIVS 428
            +K G + G     FIFA++AN TYVGSILVRTTEW++IK N+PWLLDA VCV LDLFII+QYI Y+Y R    ++   D YG+Y   +  KT VS
Sbjct:  300 IKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFIILQYIYYKYCRIKSLESREEDAYGDYV--EASKTFVS 392

HSP 2 Score: 44.2838 bits (103), Expect = 4.423e-27
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 1

Query:   22 YIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            Y   + R+LL+ E    HSALGQWLGWLMAAIY
Sbjct:  257 YAHASSRRLLN-ERIVEHSALGQWLGWLMAAIY 288

[Close]

BLAST of FG538367 vs. TrEMBL
Match: O23198_ARATH (Putative uncharacterized protein AT4g36850 OS=Arabidopsis thaliana GN=C7A10.510 PE=4 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 4.427e-27
Identity = 54/95 (56.84%), Postives = 67/95 (70.53%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHR----KTSANDGYGNYENHKVRKTIVS 428
            +K G + G     FIFA++AN TYVGSILVRTTEW++IK N+PWLLDA VCV LDLFII+QYI Y+Y R    ++   D YG+Y   +  KT VS
Sbjct:  282 IKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFIILQYIYYKYCRIKSLESREEDAYGDYV--EASKTFVS 374

HSP 2 Score: 44.2838 bits (103), Expect = 4.427e-27
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 1

Query:   22 YIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            Y   + R+LL+ E    HSALGQWLGWLMAAIY
Sbjct:  239 YAHASSRRLLN-ERIVEHSALGQWLGWLMAAIY 270

[Close]

BLAST of FG538367 vs. TrEMBL
Match: A7Y7J3_PRUDU (Putative PQ-loop repeat family protein (Fragment) OS=Prunus dulcis PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 4.721e-27
Identity = 42/61 (68.85%), Postives = 50/61 (81.97%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQ 338
            +K G + G     F+FA++AN TYVGSI+VRTTEW+SIKANMPWLLDA VCV LDLFII+Q
Sbjct:   44 IKRGTVEGLNPLMFVFALVANVTYVGSIVVRTTEWDSIKANMPWLLDAVVCVGLDLFIILQ 104

HSP 2 Score: 56.6102 bits (135), Expect = 4.721e-27
Identity = 26/33 (78.79%), Postives = 28/33 (84.85%), Query Frame = 1

Query:   22 YIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            YI +TGRKLL QEH+  HSA GQWLGWLMAAIY
Sbjct:    1 YIGITGRKLL-QEHSMEHSAFGQWLGWLMAAIY 32

[Close]

BLAST of FG538367 vs. TrEMBL
Match: D7MB35_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_490949 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.502e-27
Identity = 55/95 (57.89%), Postives = 66/95 (69.47%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRKTS----ANDGYGNYENHKVRKTIVS 428
            +K G + G     FIFA++AN TYVGSILVRTTEW++IK N+PWLLDA VCV LDLFII+QYI Y+Y R  S      D YG+Y   +  KT VS
Sbjct:  283 IKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFIILQYIYYKYCRMQSLERKEEDVYGDYV--EASKTFVS 375

HSP 2 Score: 44.2838 bits (103), Expect = 7.502e-27
Identity = 22/38 (57.89%), Postives = 26/38 (68.42%), Query Frame = 1

Query:    7 ALKVGYIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            +L   Y   + R+LL+ E    HSALGQWLGWLMAAIY
Sbjct:  235 SLAENYWHASSRRLLN-ERRVEHSALGQWLGWLMAAIY 271

[Close]

BLAST of FG538367 vs. TrEMBL
Match: C0Z2N7_ARATH (AT4G36850 protein OS=Arabidopsis thaliana GN=AT4G36850 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.679e-21
Identity = 38/58 (65.52%), Postives = 46/58 (79.31%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFI 329
            +K G + G     FIFA++AN TYVGSILVRTTEW++IK N+PWLLDA VCV LDLF+
Sbjct:  300 IKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFV 357

HSP 2 Score: 44.2838 bits (103), Expect = 6.679e-21
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 1

Query:   22 YIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            Y   + R+LL+ E    HSALGQWLGWLMAAIY
Sbjct:  257 YAHASSRRLLN-ERIVEHSALGQWLGWLMAAIY 288

[Close]

BLAST of FG538367 vs. TrEMBL
Match: Q5NBM2_ORYSJ (Os01g0266800 protein OS=Oryza sativa subsp. japonica GN=P0011D01.8 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.467e-20
Identity = 30/57 (52.63%), Postives = 44/57 (77.19%), Query Frame = 3

Query:  195 FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRK 365
            F FA++ N TYVGSILV++ +W  +K N+PWL+DAG CV LD FII+Q++ + Y ++
Sbjct:  352 FTFALVGNVTYVGSILVKSMDWSKLKPNLPWLVDAGGCVLLDTFIILQFLYFHYRKR 408

HSP 2 Score: 46.595 bits (109), Expect = 1.467e-20
Identity = 19/31 (61.29%), Postives = 26/31 (83.87%), Query Frame = 2

Query:  107 WPLSI--SGGRIPQIWLNIKRGSVEGLNPLI 193
            W +++   GGR+PQIWLN+KRG+ EGLNPL+
Sbjct:  321 WAMAVIYMGGRLPQIWLNMKRGNAEGLNPLM 351

[Close]

BLAST of FG538367 vs. TAIR peptide
Match: AT4G36850.1 (| Symbols: | PQ-loop repeat family protein / transmembrane family protein | chr4:17353415-17355822 REVERSE LENGTH=392)

HSP 1 Score: 101.293 bits (251), Expect = 3.521e-29
Identity = 54/95 (56.84%), Postives = 67/95 (70.53%), Query Frame = 3

Query:  156 LKEGVLRG*ILS*FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHR----KTSANDGYGNYENHKVRKTIVS 428
            +K G + G     FIFA++AN TYVGSILVRTTEW++IK N+PWLLDA VCV LDLFII+QYI Y+Y R    ++   D YG+Y   +  KT VS
Sbjct:  300 IKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFIILQYIYYKYCRIKSLESREEDAYGDYV--EASKTFVS 392

HSP 2 Score: 44.2838 bits (103), Expect = 3.521e-29
Identity = 21/33 (63.64%), Postives = 24/33 (72.73%), Query Frame = 1

Query:   22 YIALTGRKLLSQEHATHHSALGQWLGWLMAAIY 120
            Y   + R+LL+ E    HSALGQWLGWLMAAIY
Sbjct:  257 YAHASSRRLLN-ERIVEHSALGQWLGWLMAAIY 288

HSP 3 Score: 50.447 bits (119), Expect = 7.431e-7
Identity = 22/23 (95.65%), Postives = 23/23 (100.00%), Query Frame = 2

Query:  125 GGRIPQIWLNIKRGSVEGLNPLI 193
            GGRIPQIWLNIKRGSVEGLNPL+
Sbjct:  290 GGRIPQIWLNIKRGSVEGLNPLM 312

[Close]

BLAST of FG538367 vs. TAIR peptide
Match: AT4G20100.1 (| Symbols: | PQ-loop repeat family protein / transmembrane family protein | chr4:10873999-10874865 REVERSE LENGTH=288)

HSP 1 Score: 66.6254 bits (161), Expect = 3.135e-21
Identity = 30/61 (49.18%), Postives = 40/61 (65.57%), Query Frame = 3

Query:  195 FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYRYHRKTSAN 377
            F FA I N TYV SILV + EW  I+ N+PWL+D+G C  LD  I++Q+  Y + RK  A+
Sbjct:  216 FFFAFIGNVTYVASILVNSVEWSKIEPNLPWLVDSGGCAVLDFLILLQFF-YFHCRKVEAD 275

HSP 2 Score: 41.2022 bits (95), Expect = 3.135e-21
Identity = 16/23 (69.57%), Postives = 22/23 (95.65%), Query Frame = 2

Query:  125 GGRIPQIWLNIKRGSVEGLNPLI 193
            GGR+PQI +N++RG+VEGLNPL+
Sbjct:  193 GGRLPQICMNVRRGNVEGLNPLM 215

HSP 3 Score: 30.8018 bits (68), Expect = 3.135e-21
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query:   40 RKLL--SQEHATHHSALGQWLGWLMAAIY 120
            RKLL  S  +   ++ +G WLGW MAAIY
Sbjct:  163 RKLLEVSSGNLGENNNIGMWLGWAMAAIY 191

[Close]

BLAST of FG538367 vs. TAIR peptide
Match: AT2G41050.1 (| Symbols: | PQ-loop repeat family protein / transmembrane family protein | chr2:17123388-17125622 REVERSE LENGTH=376)

HSP 1 Score: 67.781 bits (164), Expect = 1.902e-20
Identity = 27/53 (50.94%), Postives = 38/53 (71.70%), Query Frame = 3

Query:  195 FIFAMIANTTYVGSILVRTTEWESIKANMPWLLDAGVCVALDLFIIMQYINYR 353
            F FA++ N TYV SILV + EW  +  N+PWL+DAG CV LD  I++Q+ ++R
Sbjct:  306 FFFALVGNMTYVASILVNSVEWLKLAPNLPWLVDAGGCVVLDFLILLQFFHFR 358

HSP 2 Score: 40.4318 bits (93), Expect = 1.902e-20
Identity = 17/23 (73.91%), Postives = 21/23 (91.30%), Query Frame = 2

Query:  125 GGRIPQIWLNIKRGSVEGLNPLI 193
            GGR+PQI LN++RG VEGLNPL+
Sbjct:  283 GGRLPQICLNMRRGHVEGLNPLM 305

HSP 3 Score: 27.7202 bits (60), Expect = 1.902e-20
Identity = 16/40 (40.00%), Postives = 22/40 (55.00%), Query Frame = 1

Query:   22 YIALTGRKLLS------QEHATHHSA-LGQWLGWLMAAIY 120
            ++    RKLL        EH+   S+ +G +LGW MAAIY
Sbjct:  242 FVVRAARKLLQVTSSNVAEHSGGESSRIGMFLGWAMAAIY 281

The following BLAST results are available for this feature:

BLAST of FG538367 vs. TrEMBL
BLAST of FG538367 vs. TAIR peptide

BLAST of FG538367 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FIG7_MEDTR	2.447e-45	73.91	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TMI0_SOYBN	3.600e-38	76.56	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9T4V4_RICCO	1.333e-29	63.41	Putative uncharacterized protein OS=Ricinus commun...	[more]
D7SPK1_VITVI	9.090e-28	64.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q94AH7_ARATH	4.423e-27	56.84	Putative uncharacterized protein At4g36850 OS=Arab...	[more]
O23198_ARATH	4.427e-27	56.84	Putative uncharacterized protein AT4g36850 OS=Arab...	[more]
A7Y7J3_PRUDU	4.721e-27	68.85	Putative PQ-loop repeat family protein (Fragment) ...	[more]
D7MB35_ARALY	7.502e-27	57.89	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C0Z2N7_ARATH	6.679e-21	65.52	AT4G36850 protein OS=Arabidopsis thaliana GN=AT4G3...	[more]
Q5NBM2_ORYSJ	1.467e-20	52.63	Os01g0266800 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of FG538367 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G36850.1	3.521e-29	56.84	\| Symbols: \| PQ-loop repeat family protein / tran...	[more]
AT4G20100.1	3.135e-21	49.18	\| Symbols: \| PQ-loop repeat family protein / tran...	[more]
AT2G41050.1	1.902e-20	50.94	\| Symbols: \| PQ-loop repeat family protein / tran...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF04193	PQ-loop	coord: 31..53 score: 9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG538367 ID=FG538367; Name=FG538367; organism=Pisum sativum; type=EST; length=561bp
GGTCATGCTTTGAAAGTGGGATACATAGCACTGACAGGAAGAAAACTACT
CTCCCAAGAACACGCAACACATCACAGTGCATTGGGTCAATGGTTGGGAT
GGCTCATGGCCGCTATCTATTAGCGGCGGTCGAATACCTCAAATATGGTT
AAATATTAAAAGAGGGAGTGTTGAGGGGTTAAATCCTCTCATAGTTCATC
TTTGCAATGATTGCCAATACCACTTATGTGGGAAGTATTCTTGTAAGAAC
TACGGAATGGGAAAGTATCAAAGCTAATATGCCATGGTTATTGGATGCAG
GAGTTTGTGTTGCATTGGACCTATTTATAATAATGCAGTACATCAATTAC
AGATACCATCGGAAGACAAGTGCAAATGATGGTTATGGTAACTATGAAAA
CCACAAAGTGAGAAAAACTATAGTTTCTTGATTTGTGGACATGTAACATT
AATTTTGATTTGAATTAGAACAATGTTATAGTTTAATTTTAGTTCACAAT
AACATTCAGCTCTTAGTGGTTGGGTCTTGCTATAATATGATGTGGTGTAG
GGATATTTAAT

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