FG538837

EST Overview


Name	FG538837
Unique Name	FG538837
Type	EST
Organism	Pisum sativum (pea)
Sequence length	500

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG538837 vs. TrEMBL
Match: B9SXJ3_RICCO (Heat shock protein binding protein, putative OS=Ricinus communis GN=RCOM_0503950 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.354e-26
Identity = 58/65 (89.23%), Postives = 60/65 (92.31%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            +L SDRLALGL ASGPLK+EDVK AYRACALKWHPDRHQGSSK IAEEKFK CSAAYQSLCDKLA
Sbjct:  186 DLASDRLALGLSASGPLKIEDVKNAYRACALKWHPDRHQGSSKAIAEEKFKLCSAAYQSLCDKLA 250

[Close]

BLAST of FG538837 vs. TrEMBL
Match: C6T9H2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.193e-25
Identity = 58/67 (86.57%), Postives = 61/67 (91.04%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            +LVSDRLALGL ASG LKLEDVK AYR CALKWHPDRHQGSSK IAEEKFK CSAAYQSLCDKLA++
Sbjct:  188 DLVSDRLALGLSASGSLKLEDVKNAYRVCALKWHPDRHQGSSKAIAEEKFKLCSAAYQSLCDKLALD 254

[Close]

BLAST of FG538837 vs. TrEMBL
Match: D7TID7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033493001 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.544e-24
Identity = 53/67 (79.10%), Postives = 59/67 (88.06%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            +L S R+ALGL  SGPLKLE+VK AYR CALKWHPDRHQG+SK IAEEKFK CSAAYQSLCDKLA++
Sbjct:  195 DLASHRMALGLSVSGPLKLEEVKNAYRTCALKWHPDRHQGASKAIAEEKFKLCSAAYQSLCDKLAVD 261

[Close]

BLAST of FG538837 vs. TrEMBL
Match: A5BK80_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009634 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.544e-24
Identity = 53/67 (79.10%), Postives = 59/67 (88.06%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            +L S R+ALGL  SGPLKLE+VK AYR CALKWHPDRHQG+SK IAEEKFK CSAAYQSLCDKLA++
Sbjct:  272 DLASHRMALGLSVSGPLKLEEVKNAYRTCALKWHPDRHQGASKAIAEEKFKLCSAAYQSLCDKLAVD 338

[Close]

BLAST of FG538837 vs. TrEMBL
Match: C0HGB5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.365e-21
Identity = 49/60 (81.67%), Postives = 52/60 (86.67%), Query Frame = 3

Query:   75 RLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            R ALGL  SGPLKLEDVK AYRACAL+WHPDRH GSSK  AEEKFKHCSAAY++LCD LA
Sbjct:  197 RQALGLSTSGPLKLEDVKSAYRACALRWHPDRHNGSSKSTAEEKFKHCSAAYKTLCDSLA 256

[Close]

BLAST of FG538837 vs. TrEMBL
Match: Q0D6K5_ORYSJ (Os07g0470800 protein OS=Oryza sativa subsp. japonica GN=Os07g0470800 PE=4 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 1.782e-21
Identity = 48/60 (80.00%), Postives = 51/60 (85.00%), Query Frame = 3

Query:   75 RLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            R ALGL  SGPLKLEDVK AYR CAL+WHPDRH GS+K  AEEKFKHCSAAYQ+LCD LA
Sbjct:  193 RQALGLSTSGPLKLEDVKSAYRTCALRWHPDRHNGSTKATAEEKFKHCSAAYQTLCDSLA 252

[Close]

BLAST of FG538837 vs. TrEMBL
Match: A3BJL2_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_24189 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.782e-21
Identity = 48/60 (80.00%), Postives = 51/60 (85.00%), Query Frame = 3

Query:   75 RLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            R ALGL  SGPLKLEDVK AYR CAL+WHPDRH GS+K  AEEKFKHCSAAYQ+LCD LA
Sbjct:  208 RQALGLSTSGPLKLEDVKSAYRTCALRWHPDRHNGSTKATAEEKFKHCSAAYQTLCDSLA 267

[Close]

BLAST of FG538837 vs. TrEMBL
Match: A2YL48_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_25938 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.970e-21
Identity = 48/60 (80.00%), Postives = 50/60 (83.33%), Query Frame = 3

Query:   75 RLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            R ALGL  SGPLKLEDVK AYR CAL+WHPDRH GS+K  AEEKFKHCSAAYQ LCD LA
Sbjct:  191 RQALGLSTSGPLKLEDVKSAYRTCALRWHPDRHNGSTKATAEEKFKHCSAAYQILCDSLA 250

[Close]

BLAST of FG538837 vs. TrEMBL
Match: D7LVZ1_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_666682 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.185e-21
Identity = 50/67 (74.63%), Postives = 55/67 (82.09%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            N  S R ALGL +SGPL LEDVKIAYRACALKWHPDRH  S+K  AEEKFK C+ AYQSLC+KLA+N
Sbjct:  184 NQESHRQALGLSSSGPLNLEDVKIAYRACALKWHPDRHHASTKNEAEEKFKLCTVAYQSLCEKLAMN 250

[Close]

BLAST of FG538837 vs. TrEMBL
Match: P93739_ARATH (Putative uncharacterized protein At2g42080 OS=Arabidopsis thaliana GN=At2g42080 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.772e-21
Identity = 49/67 (73.13%), Postives = 54/67 (80.60%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            N VS R ALGL  SGPL L+DVK AYR CALKWHPDRHQGS+K  AE KFK CS AYQSLC+KL++N
Sbjct:  197 NQVSHRQALGLSPSGPLNLKDVKHAYRTCALKWHPDRHQGSTKEAAEAKFKLCSVAYQSLCEKLSVN 263

[Close]

BLAST of FG538837 vs. TAIR peptide
Match: AT2G42080.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr2:17553409-17555475 REVERSE LENGTH=263)

HSP 1 Score: 103.605 bits (257), Expect = 5.887e-23
Identity = 49/67 (73.13%), Postives = 54/67 (80.60%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            N VS R ALGL  SGPL L+DVK AYR CALKWHPDRHQGS+K  AE KFK CS AYQSLC+KL++N
Sbjct:  197 NQVSHRQALGLSPSGPLNLKDVKHAYRTCALKWHPDRHQGSTKEAAEAKFKLCSVAYQSLCEKLSVN 263

[Close]

BLAST of FG538837 vs. TAIR peptide
Match: AT3G58020.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr3:21479501-21480981 FORWARD LENGTH=254)

HSP 1 Score: 101.679 bits (252), Expect = 2.237e-22
Identity = 49/67 (73.13%), Postives = 54/67 (80.60%), Query Frame = 3

Query:   60 NLVSDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLAIN 260
            N  S R  LGL +SGPL LEDVKIAYRACALKWHPDRH  S+K  AEEKFK C+ AYQSLC+KLA+N
Sbjct:  188 NQESHRQTLGLSSSGPLNLEDVKIAYRACALKWHPDRHHTSTKNEAEEKFKLCTVAYQSLCEKLAMN 254

[Close]

BLAST of FG538837 vs. TAIR peptide
Match: AT2G18465.1 (| Symbols: | Chaperone DnaJ-domain superfamily protein | chr2:8003223-8004742 REVERSE LENGTH=268)

HSP 1 Score: 85.1149 bits (209), Expect = 2.167e-17
Identity = 38/62 (61.29%), Postives = 49/62 (79.03%), Query Frame = 3

Query:   69 SDRLALGLRASGPLKLEDVKIAYRACALKWHPDRHQGSSKVIAEEKFKHCSAAYQSLCDKLA 254
            S+R+ LGL   GP+K++DVK A+R+ ALKWHPD+HQG S+V A+EKFK C  AY+SLC  LA
Sbjct:  207 SERIVLGLPLDGPIKVDDVKNAFRSSALKWHPDKHQGPSQVAAQEKFKLCVDAYKSLCSALA 268

The following BLAST results are available for this feature:

BLAST of FG538837 vs. TrEMBL
BLAST of FG538837 vs. TAIR peptide

BLAST of FG538837 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SXJ3_RICCO	5.354e-26	89.23	Heat shock protein binding protein, putative OS=Ri...	[more]
C6T9H2_SOYBN	1.193e-25	86.57	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TID7_VITVI	6.544e-24	79.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BK80_VITVI	6.544e-24	79.10	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C0HGB5_MAIZE	1.365e-21	81.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q0D6K5_ORYSJ	1.782e-21	80.00	Os07g0470800 protein OS=Oryza sativa subsp. japoni...	[more]
A3BJL2_ORYSJ	1.782e-21	80.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2YL48_ORYSI	3.970e-21	80.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7LVZ1_ARALY	5.185e-21	74.63	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
P93739_ARATH	6.772e-21	73.13	Putative uncharacterized protein At2g42080 OS=Arab...	[more]

BLAST of FG538837 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G42080.1	5.887e-23	73.13	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT3G58020.1	2.237e-22	73.13	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]
AT2G18465.1	2.167e-17	61.29	\| Symbols: \| Chaperone DnaJ-domain superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 24..33 score
None	No IPR available	PANTHER	PTHR11821:SF88	DNAJ HOMOLOG SUBFAMILY B MEMBER 4, 5	coord: 39..82 score: 1.9
None	No IPR available	SEG	seg	seg	coord: 1..19 score
None	No IPR available	SEG	seg	seg	coord: 36..48 score
None	No IPR available	SEG	seg	seg	coord: 42..49 score
IPR001623	Heat shock protein DnaJ, N-terminal	GENE3D	1.10.287.110	no description	coord: 37..82 score: 8
IPR001623	Heat shock protein DnaJ, N-terminal	PFAM	PF00226	DnaJ	coord: 37..83 score: 7.8
IPR001623	Heat shock protein DnaJ, N-terminal	SMART	SM00271	no description	coord: 22..84 score: 1.9
IPR001623	Heat shock protein DnaJ, N-terminal	PROFILE	PS50076	DNAJ_2	coord: 23..84 score: 10
IPR001623	Heat shock protein DnaJ, N-terminal	SUPERFAMILY	46565	Chaperone J-domain	coord: 39..81 score: 3.5
IPR015609	Molecular chaperone, heat shock protein, Hsp40, DnaJ	PANTHER	PTHR11821	DNAJ/HSP40	coord: 39..82 score: 1.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG538837 ID=FG538837; Name=FG538837; organism=Pisum sativum; type=EST; length=500bp
AGGGATACGGCTCCTCAACTGGGTCCGAGTCTGAGTCCGAGTCTGATAGT
TTGCGTTCAAATTTAGTCTCTGATAGATTAGCCCTTGGATTGAGAGCTTC
TGGTCCTCTGAAACTCGAAGATGTGAAGATTGCGTACCGTGCATGCGCCC
TTAAATGGCATCCTGATCGTCATCAAGGCTCTTCCAAGGTTATAGCAGAG
GAAAAGTTCAAGCATTGCAGTGCAGCTTATCAATCATTGTGTGATAAGTT
GGCAATAAATTAACAATGAGTATGCTTTTTCTACAAGTAATCAGCTCTCA
ACAGTCATTTGCGCATACTCTGATTAATTTAACTCAGCTTGCTTTCATTA
AATTATAGTTTTTGTAGTAATTTTAATGTACACTATTGGAAATAGTATAT
ATACTGTTTCACATTTAAAATTTAGTTTTAGTTCATCACCATTTCTTTTC
GCTGGGATAAACATGTTCTTAATTTGTATTCATAATGGAGTGAGGTCTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0031072	heat shock protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001623	DnaJ_domain
IPR015609	Molecular chaperone, heat shock protein, Hsp40, DnaJ

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