FG538958

EST Overview


Name	FG538958
Unique Name	FG538958
Type	EST
Organism	Pisum sativum (pea)
Sequence length	642

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG538958 vs. TrEMBL
Match: D7U799_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_13.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00013938001 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 1.244e-51
Identity = 85/106 (80.19%), Postives = 95/106 (89.62%), Query Frame = 3

Query:    9 VHTVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            +H V EKDASGN+LL DVG WLS KIKDHFT+  KMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGY+GFT+GPV+SRHAY+PI RVTE   T+++
Sbjct:  384 MHAVSEKDASGNRLLLDVGLWLSQKIKDHFTKVQKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYTGFTIGPVSSRHAYIPIDRVTETTKTIKL 489

HSP 2 Score: 47.7506 bits (112), Expect = 1.244e-51
Identity = 25/41 (60.98%), Postives = 31/41 (75.61%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFV--TPPDQQRVDKEMIKDTNKIDITS 448
            RMWARLL STNQPSF+  +P  Q+RVDKE I+  + + ITS
Sbjct:  492 RMWARLLSSTNQPSFLKGSPVMQERVDKETIEMIDNMKITS 532

[Close]

BLAST of FG538958 vs. TrEMBL
Match: B9R9Y5_RICCO (Phosphofructokinase, putative OS=Ricinus communis GN=RCOM_1501790 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 2.256e-47
Identity = 84/101 (83.17%), Postives = 91/101 (90.10%), Query Frame = 3

Query:   24 EKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            E+DASGN+LL DVG WLS  IK+HFT   KMAVNMKYIDPTYMIRA+PSNASDNIYCTLLA SAVHGAMAGY+GFTVGPVNSRHAY+PIARVTE  NTV+V
Sbjct:  377 ERDASGNRLLLDVGLWLSQNIKEHFTNIQKMAVNMKYIDPTYMIRAVPSNASDNIYCTLLAQSAVHGAMAGYTGFTVGPVNSRHAYIPIARVTEATNTVKV 477

HSP 2 Score: 37.3502 bits (85), Expect = 2.256e-47
Identity = 21/42 (50.00%), Postives = 28/42 (66.67%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD--QQRVDKEMIKDTN-KIDITS 448
            RMWARLL STNQPSF+   +  Q R+D   ++  N  I++TS
Sbjct:  480 RMWARLLASTNQPSFLISKEVLQGRIDGNKVEVPNDNIEMTS 521

[Close]

BLAST of FG538958 vs. TrEMBL
Match: D7MV38_ARALY (Phosphofructokinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496324 PE=4 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 6.510e-47
Identity = 83/106 (78.30%), Postives = 93/106 (87.74%), Query Frame = 3

Query:    9 VHTVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            +H  G KDASGN+LL DVG WL+ +IKDHFT   KM +NMKYIDPTYMIRAIPSNASDN+YCTLLA SAVHGAMAGYSGFTVGPVNSRHAY+PI+RVTE  NTV++
Sbjct:  381 MHVSGTKDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISRVTEMTNTVKL 486

HSP 2 Score: 36.5798 bits (83), Expect = 6.510e-47
Identity = 20/41 (48.78%), Postives = 26/41 (63.41%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD--QQRVDKEMIKDTNKIDITS 448
            RMWARLL STNQPSF+T     Q  +D E  +  + + I+S
Sbjct:  489 RMWARLLASTNQPSFLTGEGALQNVIDMETQEKIDNMKISS 529

[Close]

BLAST of FG538958 vs. TrEMBL
Match: B9NBR1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_280952 PE=4 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.882e-46
Identity = 82/101 (81.19%), Postives = 92/101 (91.09%), Query Frame = 3

Query:   24 EKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            E+DASGN+LL DVG WL+ K+KDHFT+  KM +NMKYIDPTYMIRAIPSNASDNIYC+LLA SAVHGAMAGY+GFTVGPVNSRHAY+PIARVTE  NTV+V
Sbjct:  319 ERDASGNRLLLDVGLWLTQKVKDHFTKVKKMEINMKYIDPTYMIRAIPSNASDNIYCSLLAQSAVHGAMAGYTGFTVGPVNSRHAYIPIARVTEAQNTVKV 419

HSP 2 Score: 35.8094 bits (81), Expect = 1.882e-46
Identity = 15/17 (88.24%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  329 GRMWARLLESTNQPSFV 379
            GRMWARLL STNQPSF+
Sbjct:  421 GRMWARLLASTNQPSFL 437

[Close]

BLAST of FG538958 vs. TrEMBL
Match: C1K4P9_ELAOL (Phosphofructokinase (Fragment) OS=Elaeis oleifera GN=PFK PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 6.468e-44
Identity = 76/107 (71.03%), Postives = 90/107 (84.11%), Query Frame = 3

Query:    9 VHTVGEKDASGNKLLRDVGPWLSNKIKDHF-TQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            + ++G +DASGNKLL DVG WL  KIKDHF ++  KMA+N+KYIDPTYMIRAIPSNASDN+YCTLLAHS +HGAMAGY+GFTVGPVN RHAY+P  R+TE  N V +
Sbjct:   28 MRSMGHEDASGNKLLLDVGLWLFQKIKDHFASRKKKMAINLKYIDPTYMIRAIPSNASDNVYCTLLAHSVIHGAMAGYTGFTVGPVNGRHAYIPFHRITETQNKVVI 134

HSP 2 Score: 40.0466 bits (92), Expect = 6.468e-44
Identity = 19/36 (52.78%), Postives = 23/36 (63.89%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKEMIKDTNKID 439
            RMWARLL STNQPSF++  D +   KE    T  +D
Sbjct:  137 RMWARLLSSTNQPSFLSHKDVEDARKEEAPPTQLLD 172

[Close]

BLAST of FG538958 vs. TrEMBL
Match: B9RRX6_RICCO (Phosphofructokinase, putative OS=Ricinus communis GN=RCOM_0799050 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 4.071e-43
Identity = 73/106 (68.87%), Postives = 90/106 (84.91%), Query Frame = 3

Query:    9 VHTVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            + ++ ++DASGNKLL+DVG W+S +IKDHF +  +M + +KYIDPTYMIRAIPSNASDN+YCTLLAHSAVHGAMAGY+GFTVGPVN RHAY+P  R+TE  N V +
Sbjct:  334 LQSMNQQDASGNKLLQDVGLWISQRIKDHFAREHRMNITLKYIDPTYMIRAIPSNASDNVYCTLLAHSAVHGAMAGYTGFTVGPVNGRHAYIPFNRITEKQNNVVI 439

HSP 2 Score: 36.965 bits (84), Expect = 4.071e-43
Identity = 16/27 (59.26%), Postives = 21/27 (77.78%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKE 412
            RMWARLL ST+QPSF++P    +V K+
Sbjct:  442 RMWARLLSSTDQPSFMSPKVLTKVKKQ 468

[Close]

BLAST of FG538958 vs. TrEMBL
Match: B9RQC7_RICCO (Phosphofructokinase, putative OS=Ricinus communis GN=RCOM_1487750 PE=4 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.594e-42
Identity = 72/106 (67.92%), Postives = 90/106 (84.91%), Query Frame = 3

Query:    9 VHTVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            + T+ ++DASGN+LL+DVG W+S KIKDHF+Q  KM + +KYIDPTYMIRA+PSNASDN+YCTLLAHSA+HGAMAGY+GFTVGPVN RH+++P  RV E  N V +
Sbjct:  389 MRTMDKQDASGNRLLQDVGLWISQKIKDHFSQDQKMNITLKYIDPTYMIRAVPSNASDNVYCTLLAHSAIHGAMAGYTGFTVGPVNGRHSHIPFHRVNERRNKVVI 494

HSP 2 Score: 36.1946 bits (82), Expect = 2.594e-42
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTP 385
            RMWARLL STNQPSF+ P
Sbjct:  497 RMWARLLSSTNQPSFLDP 514

[Close]

BLAST of FG538958 vs. TrEMBL
Match: D7STJ8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035322001 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 5.747e-42
Identity = 69/100 (69.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            +DASGN LL+DVG W+S++IKDHF +  K+A+N+KYIDPTYMIRAIP NASDN+YCTLLA SAVHGAMAGY+GFT+G VN RHAY+P  R+ E  N V +
Sbjct:  336 QDASGNNLLQDVGLWMSHRIKDHFARQRKIAINLKYIDPTYMIRAIPGNASDNVYCTLLAQSAVHGAMAGYTGFTIGIVNGRHAYIPFHRINESRNKVVI 435

HSP 2 Score: 43.1282 bits (100), Expect = 5.747e-42
Identity = 21/36 (58.33%), Postives = 22/36 (61.11%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKEMIKDTNKID 439
            RMWARLL STNQPSF  P D   V KE    T  +D
Sbjct:  438 RMWARLLSSTNQPSFQNPKDVAEVKKEEAPATGLLD 473

[Close]

BLAST of FG538958 vs. TrEMBL
Match: D7TBD7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015271001 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 2.159e-41
Identity = 69/101 (68.32%), Postives = 85/101 (84.16%), Query Frame = 3

Query:   24 EKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            ++DASGNKLL+DVG W+S KI+DHF+   +M + +KYIDPTYMIRA+PSNASDN+YCTLLA SAVHGAMAGY+GFTVGPVN RH+Y+P  R+ E  N V +
Sbjct:  335 KQDASGNKLLQDVGLWISQKIRDHFSTSKRMGITLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGFTVGPVNGRHSYIPFYRIIERQNKVVI 435

HSP 2 Score: 38.891 bits (89), Expect = 2.159e-41
Identity = 16/20 (80.00%), Postives = 18/20 (90.00%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD 391
            RMWARLL STNQPSF++P D
Sbjct:  438 RMWARLLSSTNQPSFLSPED 457

[Close]

BLAST of FG538958 vs. TrEMBL
Match: A5BPE1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023326 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 2.813e-41
Identity = 68/100 (68.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            +DASGN LL+DVG W+S++IKD+F +  K+A+N+KYIDPTYMIRAIP NASDN+YCTLLA SAVHGAMAGY+GFT+G VN RHAY+P  R+ E  N V +
Sbjct:  336 QDASGNNLLQDVGLWMSHRIKDYFARQRKIAINLKYIDPTYMIRAIPGNASDNVYCTLLAQSAVHGAMAGYTGFTIGIVNGRHAYIPFHRINESRNKVVI 435

HSP 2 Score: 43.1282 bits (100), Expect = 2.813e-41
Identity = 21/36 (58.33%), Postives = 22/36 (61.11%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKEMIKDTNKID 439
            RMWARLL STNQPSF  P D   V KE    T  +D
Sbjct:  438 RMWARLLSSTNQPSFQNPKDVAEVKKEEAPATGLLD 473

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF4_ARATH (6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana GN=PFK4 PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.121e-46
Identity = 80/100 (80.00%), Postives = 90/100 (90.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGN+LL DVG WL+ +IKDHFT   KM +NMKYIDPTYMIRAIPSNASDN+YCTLLA SAVHGAMAGYSGFTVGPVNSRHAY+PI++VTE  NTV++
Sbjct:  387 KDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKL 486

HSP 2 Score: 36.5798 bits (83), Expect = 3.121e-46
Identity = 20/41 (48.78%), Postives = 26/41 (63.41%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD--QQRVDKEMIKDTNKIDITS 448
            RMWARLL STNQPSF+T     Q  +D E  +  + + I+S
Sbjct:  489 RMWARLLASTNQPSFLTGEGALQNVIDMETQEKIDNMKISS 529

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF7_ARATH (6-phosphofructokinase 7 OS=Arabidopsis thaliana GN=PFK7 PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.280e-40
Identity = 69/99 (69.70%), Postives = 80/99 (80.81%), Query Frame = 3

Query:   30 DASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            DASGNKLL+DVG WLS  IKDHF +  KM +N+KYIDPTYMIRA+PSNASDN+YCTLLA SAVHGAMAGY+G+T G VN R  Y+P  R+TE  N V +
Sbjct:  336 DASGNKLLKDVGLWLSQSIKDHFKK-NKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVI 433

HSP 2 Score: 41.5874 bits (96), Expect = 2.280e-40
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKEM----IKDTNKIDI 442
            RMWARLL STNQPSF+ P D     KE+    + D   +DI
Sbjct:  436 RMWARLLSSTNQPSFLGPKDTSEEKKELPETPLLDDGAVDI 476

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF3_ARATH (6-phosphofructokinase 3 OS=Arabidopsis thaliana GN=PFK3 PE=1 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.453e-39
Identity = 70/100 (70.00%), Postives = 80/100 (80.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGNKLL+DVG WLS  IKDHF Q  KM +N+KYIDPTYMIRA+PSNASDN+YCTLLA SAVHGAMAGY+G+  G VN R  Y+P  R+TE  N V +
Sbjct:  336 KDASGNKLLKDVGLWLSQSIKDHFNQ-KKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVI 434

HSP 2 Score: 37.7354 bits (86), Expect = 1.453e-39
Identity = 16/20 (80.00%), Postives = 17/20 (85.00%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD 391
            RMWARLL STNQPSF+ P D
Sbjct:  437 RMWARLLSSTNQPSFLGPKD 456

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF1_ARATH (6-phosphofructokinase 1 OS=Arabidopsis thaliana GN=PFK1 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 9.257e-39
Identity = 68/100 (68.00%), Postives = 81/100 (81.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGNKLL+D+G W+S +IKDHF +  KM + +KYIDPTYMIRA+PSNASDN+ CTLLA SAVHG MAGY+GFTVG VN RH Y+P  R+TE  N V +
Sbjct:  338 KDASGNKLLQDIGLWISQRIKDHFAK--KMTLTLKYIDPTYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVVI 435

HSP 2 Score: 35.4242 bits (80), Expect = 9.257e-39
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQ 394
            RMWARLL STNQPSF+   D+
Sbjct:  438 RMWARLLSSTNQPSFMKQADK 458

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF6_ARATH (6-phosphofructokinase 6 OS=Arabidopsis thaliana GN=PFK6 PE=1 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 5.897e-38
Identity = 65/104 (62.50%), Postives = 82/104 (78.85%), Query Frame = 3

Query:   15 TVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            ++ ++DASGNKLL+DVG W+S KIK++F +   M + +KYIDPTYMIRAIP+NASDN+Y TLLA SAVHGAMAGY+GF  G VN RH Y+P  R+TE  N V +
Sbjct:  330 SIEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDITLKYIDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQNKVVI 433

HSP 2 Score: 38.5058 bits (88), Expect = 5.897e-38
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPP 388
            RMWAR+L STNQPSF+ PP
Sbjct:  436 RMWARMLSSTNQPSFMNPP 454

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF2_ARATH (6-phosphofructokinase 2 OS=Arabidopsis thaliana GN=PFK2 PE=1 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.603e-26
Identity = 53/103 (51.46%), Postives = 72/103 (69.90%), Query Frame = 3

Query:   24 EKDASGNKLLRDVGPWLSNKIKDHFT-QFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEVK 329
            E+D SGN +  DVG W  + +K  +  + P     +KYIDPTYMIRA+P+NA+DN+YCTLLAHSA+HG MAGY+GF  GP+N  +AY+P+  V +  N V  +
Sbjct:  320 ERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVNTR 422

[Close]

BLAST of FG538958 vs. SwissProt
Match: K6PF5_ARATH (6-phosphofructokinase 5, chloroplastic OS=Arabidopsis thaliana GN=PFK5 PE=1 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 6.913e-25
Identity = 52/99 (52.53%), Postives = 70/99 (70.71%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVE 323
            KDASGN +L D G ++  + K +F +     +++KYIDPTYMIRA+ +NASD I CT+L  +AVHGA AGYSG TVG +N+ +AYLPI  V   P +V+
Sbjct:  421 KDASGNAVLGDFGVYIQQETKKYFKEI-STPIDVKYIDPTYMIRAVRANASDGILCTVLGQNAVHGAFAGYSGITVGIINTHYAYLPITEVIAYPKSVD 518

HSP 2 Score: 26.1794 bits (56), Expect = 6.913e-25
Identity = 10/16 (62.50%), Postives = 11/16 (68.75%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFV 379
            RMW R L ST QP F+
Sbjct:  522 RMWHRCLTSTGQPDFI 537

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT5G61580.1 (| Symbols: PFK4 | phosphofructokinase 4 | chr5:24761150-24763827 FORWARD LENGTH=530)

HSP 1 Score: 169.859 bits (429), Expect = 3.198e-47
Identity = 80/100 (80.00%), Postives = 90/100 (90.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGN+LL DVG WL+ +IKDHFT   KM +NMKYIDPTYMIRAIPSNASDN+YCTLLA SAVHGAMAGYSGFTVGPVNSRHAY+PI++VTE  NTV++
Sbjct:  387 KDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKL 486

HSP 2 Score: 36.5798 bits (83), Expect = 3.198e-47
Identity = 20/41 (48.78%), Postives = 26/41 (63.41%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD--QQRVDKEMIKDTNKIDITS 448
            RMWARLL STNQPSF+T     Q  +D E  +  + + I+S
Sbjct:  489 RMWARLLASTNQPSFLTGEGALQNVIDMETQEKIDNMKISS 529

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT5G61580.2 (| Symbols: PFK4 | phosphofructokinase 4 | chr5:24761150-24763827 FORWARD LENGTH=529)

HSP 1 Score: 164.851 bits (416), Expect = 1.000e-45
Identity = 80/100 (80.00%), Postives = 89/100 (89.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGN+LL DVG WL+ +IKDHFT   KM +NMKYIDPTYMIRAIPSNASDN+YCTLLA SAVHGAMAGYSGFTVGPVNSRHAY+PI+ VTE  NTV++
Sbjct:  387 KDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPIS-VTEVTNTVKL 485

HSP 2 Score: 36.5798 bits (83), Expect = 1.000e-45
Identity = 20/41 (48.78%), Postives = 26/41 (63.41%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD--QQRVDKEMIKDTNKIDITS 448
            RMWARLL STNQPSF+T     Q  +D E  +  + + I+S
Sbjct:  488 RMWARLLASTNQPSFLTGEGALQNVIDMETQEKIDNMKISS 528

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT5G56630.1 (| Symbols: PFK7 | phosphofructokinase 7 | chr5:22924311-22926728 FORWARD LENGTH=485)

HSP 1 Score: 145.206 bits (365), Expect = 2.336e-41
Identity = 69/99 (69.70%), Postives = 80/99 (80.81%), Query Frame = 3

Query:   30 DASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            DASGNKLL+DVG WLS  IKDHF +  KM +N+KYIDPTYMIRA+PSNASDN+YCTLLA SAVHGAMAGY+G+T G VN R  Y+P  R+TE  N V +
Sbjct:  336 DASGNKLLKDVGLWLSQSIKDHFKK-NKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVI 433

HSP 2 Score: 41.5874 bits (96), Expect = 2.336e-41
Identity = 21/41 (51.22%), Postives = 25/41 (60.98%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQQRVDKEM----IKDTNKIDI 442
            RMWARLL STNQPSF+ P D     KE+    + D   +DI
Sbjct:  436 RMWARLLSSTNQPSFLGPKDTSEEKKELPETPLLDDGAVDI 476

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT4G26270.1 (| Symbols: PFK3 | phosphofructokinase 3 | chr4:13301094-13304030 REVERSE LENGTH=489)

HSP 1 Score: 146.362 bits (368), Expect = 1.489e-40
Identity = 70/100 (70.00%), Postives = 80/100 (80.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGNKLL+DVG WLS  IKDHF Q  KM +N+KYIDPTYMIRA+PSNASDN+YCTLLA SAVHGAMAGY+G+  G VN R  Y+P  R+TE  N V +
Sbjct:  336 KDASGNKLLKDVGLWLSQSIKDHFNQ-KKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVI 434

HSP 2 Score: 37.7354 bits (86), Expect = 1.489e-40
Identity = 16/20 (80.00%), Postives = 17/20 (85.00%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPD 391
            RMWARLL STNQPSF+ P D
Sbjct:  437 RMWARLLSSTNQPSFLGPKD 456

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT4G29220.1 (| Symbols: PFK1 | phosphofructokinase 1 | chr4:14403621-14406071 REVERSE LENGTH=473)

HSP 1 Score: 145.976 bits (367), Expect = 9.486e-40
Identity = 68/100 (68.00%), Postives = 81/100 (81.00%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            KDASGNKLL+D+G W+S +IKDHF +  KM + +KYIDPTYMIRA+PSNASDN+ CTLLA SAVHG MAGY+GFTVG VN RH Y+P  R+TE  N V +
Sbjct:  338 KDASGNKLLQDIGLWISQRIKDHFAK--KMTLTLKYIDPTYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVVI 435

HSP 2 Score: 35.4242 bits (80), Expect = 9.486e-40
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPPDQ 394
            RMWARLL STNQPSF+   D+
Sbjct:  438 RMWARLLSSTNQPSFMKQADK 458

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT4G32840.1 (| Symbols: PFK6 | phosphofructokinase 6 | chr4:15845010-15848305 REVERSE LENGTH=462)

HSP 1 Score: 140.198 bits (352), Expect = 6.042e-39
Identity = 65/104 (62.50%), Postives = 82/104 (78.85%), Query Frame = 3

Query:   15 TVGEKDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEV 326
            ++ ++DASGNKLL+DVG W+S KIK++F +   M + +KYIDPTYMIRAIP+NASDN+Y TLLA SAVHGAMAGY+GF  G VN RH Y+P  R+TE  N V +
Sbjct:  330 SIEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDITLKYIDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQNKVVI 433

HSP 2 Score: 38.5058 bits (88), Expect = 6.042e-39
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFVTPP 388
            RMWAR+L STNQPSF+ PP
Sbjct:  436 RMWARMLSSTNQPSFMNPP 454

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT5G47810.1 (| Symbols: PFK2 | phosphofructokinase 2 | chr5:19356569-19357989 REVERSE LENGTH=444)

HSP 1 Score: 119.398 bits (298), Expect = 1.646e-27
Identity = 53/103 (51.46%), Postives = 72/103 (69.90%), Query Frame = 3

Query:   24 EKDASGNKLLRDVGPWLSNKIKDHFT-QFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVEVK 329
            E+D SGN +  DVG W  + +K  +  + P     +KYIDPTYMIRA+P+NA+DN+YCTLLAHSA+HG MAGY+GF  GP+N  +AY+P+  V +  N V  +
Sbjct:  320 ERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVNTR 422

[Close]

BLAST of FG538958 vs. TAIR peptide
Match: AT2G22480.1 (| Symbols: PFK5 | phosphofructokinase 5 | chr2:9545670-9548414 FORWARD LENGTH=537)

HSP 1 Score: 108.612 bits (270), Expect = 7.079e-26
Identity = 52/99 (52.53%), Postives = 70/99 (70.71%), Query Frame = 3

Query:   27 KDASGNKLLRDVGPWLSNKIKDHFTQFPKMAVNMKYIDPTYMIRAIPSNASDNIYCTLLAHSAVHGAMAGYSGFTVGPVNSRHAYLPIARVTEGPNTVE 323
            KDASGN +L D G ++  + K +F +     +++KYIDPTYMIRA+ +NASD I CT+L  +AVHGA AGYSG TVG +N+ +AYLPI  V   P +V+
Sbjct:  421 KDASGNAVLGDFGVYIQQETKKYFKEI-STPIDVKYIDPTYMIRAVRANASDGILCTVLGQNAVHGAFAGYSGITVGIINTHYAYLPITEVIAYPKSVD 518

HSP 2 Score: 26.1794 bits (56), Expect = 7.079e-26
Identity = 10/16 (62.50%), Postives = 11/16 (68.75%), Query Frame = 2

Query:  332 RMWARLLESTNQPSFV 379
            RMW R L ST QP F+
Sbjct:  522 RMWHRCLTSTGQPDFI 537

The following BLAST results are available for this feature:

BLAST of FG538958 vs. TrEMBL
BLAST of FG538958 vs. SwissProt
BLAST of FG538958 vs. TAIR peptide

BLAST of FG538958 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7U799_VITVI	1.244e-51	80.19	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9R9Y5_RICCO	2.256e-47	83.17	Phosphofructokinase, putative OS=Ricinus communis ...	[more]
D7MV38_ARALY	6.510e-47	78.30	Phosphofructokinase family protein OS=Arabidopsis ...	[more]
B9NBR1_POPTR	1.882e-46	81.19	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
C1K4P9_ELAOL	6.468e-44	71.03	Phosphofructokinase (Fragment) OS=Elaeis oleifera ...	[more]
B9RRX6_RICCO	4.071e-43	68.87	Phosphofructokinase, putative OS=Ricinus communis ...	[more]
B9RQC7_RICCO	2.594e-42	67.92	Phosphofructokinase, putative OS=Ricinus communis ...	[more]
D7STJ8_VITVI	5.747e-42	69.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TBD7_VITVI	2.159e-41	68.32	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BPE1_VITVI	2.813e-41	68.00	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of FG538958 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
K6PF4_ARATH	3.121e-46	80.00	6-phosphofructokinase 4, chloroplastic OS=Arabidop...	[more]
K6PF7_ARATH	2.280e-40	69.70	6-phosphofructokinase 7 OS=Arabidopsis thaliana GN...	[more]
K6PF3_ARATH	1.453e-39	70.00	6-phosphofructokinase 3 OS=Arabidopsis thaliana GN...	[more]
K6PF1_ARATH	9.257e-39	68.00	6-phosphofructokinase 1 OS=Arabidopsis thaliana GN...	[more]
K6PF6_ARATH	5.897e-38	62.50	6-phosphofructokinase 6 OS=Arabidopsis thaliana GN...	[more]
K6PF2_ARATH	1.603e-26	51.46	6-phosphofructokinase 2 OS=Arabidopsis thaliana GN...	[more]
K6PF5_ARATH	6.913e-25	52.53	6-phosphofructokinase 5, chloroplastic OS=Arabidop...	[more]

BLAST of FG538958 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 8


Match Name	E-value	Identity	Description
AT5G61580.1	3.198e-47	80.00	\| Symbols: PFK4 \| phosphofructokinase 4 \| chr5:247...	[more]
AT5G61580.2	1.000e-45	80.00	\| Symbols: PFK4 \| phosphofructokinase 4 \| chr5:247...	[more]
AT5G56630.1	2.336e-41	69.70	\| Symbols: PFK7 \| phosphofructokinase 7 \| chr5:229...	[more]
AT4G26270.1	1.489e-40	70.00	\| Symbols: PFK3 \| phosphofructokinase 3 \| chr4:133...	[more]
AT4G29220.1	9.486e-40	68.00	\| Symbols: PFK1 \| phosphofructokinase 1 \| chr4:144...	[more]
AT4G32840.1	6.042e-39	62.50	\| Symbols: PFK6 \| phosphofructokinase 6 \| chr4:158...	[more]
AT5G47810.1	1.646e-27	51.46	\| Symbols: PFK2 \| phosphofructokinase 2 \| chr5:193...	[more]
AT2G22480.1	7.079e-26	52.53	\| Symbols: PFK5 \| phosphofructokinase 5 \| chr2:954...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000023	Phosphofructokinase domain	SUPERFAMILY	53784	Phosphofructokinase	coord: 4..109 score: 5.9
None	No IPR available	GENE3D	3.40.50.460	no description	coord: 9..101 score: 2.3
None	No IPR available	PANTHER	PTHR13697	PHOSPHOFRUCTOKINASE	coord: 8..108 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG538958 ID=FG538958; Name=FG538958; organism=Pisum sativum; type=EST; length=642bp
GTGCAGAAGTGCATACAGTAGGTGAGAAGGATGCATCAGGGAACAAGCTA
CTTCGTGATGTTGGTCCGTGGTTATCCAATAAGATCAAGGATCATTTCAC
ACAGTTTCCTAAAATGGCGGTAAATATGAAATATATAGATCCTACATACA
TGATTCGAGCTATTCCAAGTAATGCCTCGGACAACATTTACTGCACTCTT
CTTGCTCATAGTGCTGTTCATGGAGCTATGGCAGGATATTCTGGCTTCAC
AGTCGGGCCTGTGAATAGTAGACATGCTTATCTCCCAATCGCTCGTGTGA
CAGAGGGACCAAATACAGTTGAGGTAAAGGTAGGATGTGGGCTAGACTTC
TTGAGTCGACAAATCAACCAAGTTTTGTTACCCCTCCTGACCAACAAAGA
GTTGACAAAGAGATGATCAAAGATACAAATAAGATCGATATTACCTCTAC
CTAATTTGGGTTTAATAATATGTATCAGCTATTGCTGCAAATTTACTAGC
CTTTTGCGTTAAATCATATTCTGGCTGATACTTCATGTTCTTTTTATTTA
TTTTTTAGTTGGGAAAATTAGAATATGGTAGATTTATATACGGGCATTAT
GTGTTCAACTATTCCTGTAAACTATCGGATTTTTTAATAGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003872	6-phosphofructokinase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005945	6-phosphofructokinase complex

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000023	Phosphofructokinase_dom

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