FG529054

EST Overview


Name	FG529054
Unique Name	FG529054
Type	EST
Organism	Pisum sativum (pea)
Sequence length	593

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG529054 vs. TrEMBL
Match: C5YAH3_SORBI (Putative uncharacterized protein Sb06g032800 OS=Sorghum bicolor GN=Sb06g032800 PE=4 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 4.428e-99
Identity = 170/181 (93.92%), Postives = 176/181 (97.24%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            PDAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYR+DKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  540 PDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRIDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 720

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BLAST of FG529054 vs. TrEMBL
Match: C0P8G5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 4.428e-99
Identity = 170/181 (93.92%), Postives = 176/181 (97.24%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            PDAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYR+DKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  539 PDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRIDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 719

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BLAST of FG529054 vs. TrEMBL
Match: B6T514_MAIZE (Oligosaccharyl transferase STT3 subunit OS=Zea mays PE=2 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 4.428e-99
Identity = 170/181 (93.92%), Postives = 176/181 (97.24%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            PDAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYR+DKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  540 PDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRIDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 720

[Close]

BLAST of FG529054 vs. TrEMBL
Match: Q7XQ88_ORYSJ (OSJNBa0018M05.20 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0018M05.20 PE=2 SV=2)

HSP 1 Score: 361.688 bits (927), Expect = 2.198e-98
Identity = 170/180 (94.44%), Postives = 175/180 (97.22%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            DAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  542 DAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 721

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BLAST of FG529054 vs. TrEMBL
Match: Q259H1_ORYSA (H0103C06.8 protein OS=Oryza sativa GN=H0103C06.8 PE=4 SV=1)

HSP 1 Score: 361.688 bits (927), Expect = 2.198e-98
Identity = 170/180 (94.44%), Postives = 175/180 (97.22%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            DAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  542 DAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 721

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BLAST of FG529054 vs. TrEMBL
Match: A2XYX2_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_17905 PE=4 SV=1)

HSP 1 Score: 361.688 bits (927), Expect = 2.198e-98
Identity = 170/180 (94.44%), Postives = 175/180 (97.22%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            DAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPPKNRS
Sbjct:  542 DAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPKNRS 721

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BLAST of FG529054 vs. TrEMBL
Match: D7TXQ0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_53.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029339001 PE=4 SV=1)

HSP 1 Score: 360.147 bits (923), Expect = 6.394e-98
Identity = 171/180 (95.00%), Postives = 173/180 (96.11%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IMRSLDVDYVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKL YYRFGEL TEYGKP GYDRARGVEIGNKDIKLEYLEEA TT NWIVRIYKVKPP NR
Sbjct:  374 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMRSLDVDYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLSYYRFGELQTEYGKPPGYDRARGVEIGNKDIKLEYLEEAFTTSNWIVRIYKVKPPNNR 553

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BLAST of FG529054 vs. TrEMBL
Match: D0ABB6_9ORYZ (OO_Ba0005L10-OO_Ba0081K17.25 protein OS=Oryza officinalis GN=OO_Ba0005L10-OO_Ba0081K17.25 PE=4 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.350e-98
Identity = 169/180 (93.89%), Postives = 175/180 (97.22%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            DAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKP GYDR RGVEIGNKDIKLE+LEEA TT NWIVRIYKVKPPKNRS
Sbjct:  528 DAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMQSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRVRGVEIGNKDIKLEHLEEAFTTSNWIVRIYKVKPPKNRS 707

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BLAST of FG529054 vs. TrEMBL
Match: B9N358_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_743464 PE=4 SV=1)

HSP 1 Score: 358.607 bits (919), Expect = 1.860e-97
Identity = 170/180 (94.44%), Postives = 173/180 (96.11%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IMRSLDVDYVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKL YYRFGEL TEYGKP GYDRARGVEIGNKDIKLE+LEEA TT NWIVRIYKVKPP NR
Sbjct:  541 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMRSLDVDYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLSYYRFGELVTEYGKPPGYDRARGVEIGNKDIKLEHLEEAFTTSNWIVRIYKVKPPNNR 720

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BLAST of FG529054 vs. TrEMBL
Match: B9I4Z5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1094997 PE=4 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 4.144e-97
Identity = 169/180 (93.89%), Postives = 173/180 (96.11%), Query Frame = 1

Query:    4 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAY+IM+SLDVDYVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGEYRVDKGAAPKMLNCLMYKL YYRFGEL TEYGKP GYDRARGVEIGNKDIKLE+LEEA TT NWIVRIYKVKPP NR
Sbjct:  541 PDAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMKSLDVDYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLSYYRFGELVTEYGKPPGYDRARGVEIGNKDIKLEHLEEAFTTSNWIVRIYKVKPPNNR 720

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BLAST of FG529054 vs. SwissProt
Match: STT3_DICDI (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Dictyostelium discoideum GN=stt3 PE=3 SV=1)

HSP 1 Score: 270.781 bits (691), Expect = 3.682e-72
Identity = 123/180 (68.33%), Postives = 151/180 (83.89%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS 546
            DA++MSWWDYGYQ++AM NRTV+VDNNTWNN+HIA VG+A +S E++AY  M++LDVDYVLV+FGG+TGYS+DDINKFLWMVRIGG   P IKE  YL NG+YR+DKGA+P MLN LMYKL YYRF E+ T+Y +PTG+DR R VEIGNK+  L YLEEA T+ +W+VR+YKVK   NR+
Sbjct:  535 DARIMSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYIQMKALDVDYVLVIFGGLTGYSSDDINKFLWMVRIGGSCDPNIKEQDYLTNGQYRIDKGASPTMLNSLMYKLSYYRFSEVHTDYQRPTGFDRVRNVEIGNKNFDLTYLEEAFTSVHWLVRVYKVKDFDNRA 714

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BLAST of FG529054 vs. SwissProt
Match: STT3A_BOVIN (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Bos taurus GN=STT3A PE=2 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 1.184e-70
Identity = 122/182 (67.03%), Postives = 148/182 (81.32%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP---VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            DAKVMSWWDYGYQITAM NRT++VDNNTWNNTHI+ VG+AM+S E++AY+IMR LDV YVLV+FGG+TGYS+DDINKFLWMVRIGG       + +  YY   GE+RVD+  +P +LNCLMYK+CYYRFG++ TE  +P GYDR R  EIGNKD +L+ LEEA TT++W+VRIYKVK   NR
Sbjct:  519 DAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKEHDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPLGYDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR 700

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BLAST of FG529054 vs. SwissProt
Match: STT3A_PONAB (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Pongo abelii GN=STT3A PE=2 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 3.446e-70
Identity = 121/182 (66.48%), Postives = 148/182 (81.32%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP---VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            DAKVMSWWDYGYQITAM NRT++VDNNTWNNTHI+ VG+AM+S E++AY+IMR LDV YVLV+FGG+TGYS+DDINKFLWMVRIGG       + +  YY   GE+RVD+  +P +LNCLMYK+CYYRFG++ TE  +P G+DR R  EIGNKD +L+ LEEA TT++W+VRIYKVK   NR
Sbjct:  519 DAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR 700

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BLAST of FG529054 vs. SwissProt
Match: STT3A_MOUSE (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Mus musculus GN=Stt3a PE=1 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 3.446e-70
Identity = 121/182 (66.48%), Postives = 148/182 (81.32%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP---VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            DAKVMSWWDYGYQITAM NRT++VDNNTWNNTHI+ VG+AM+S E++AY+IMR LDV YVLV+FGG+TGYS+DDINKFLWMVRIGG       + +  YY   GE+RVD+  +P +LNCLMYK+CYYRFG++ TE  +P G+DR R  EIGNKD +L+ LEEA TT++W+VRIYKVK   NR
Sbjct:  519 DAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR 700

[Close]

BLAST of FG529054 vs. SwissProt
Match: STT3A_HUMAN (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Homo sapiens GN=STT3A PE=1 SV=2)

HSP 1 Score: 264.233 bits (674), Expect = 3.446e-70
Identity = 121/182 (66.48%), Postives = 148/182 (81.32%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP---VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            DAKVMSWWDYGYQITAM NRT++VDNNTWNNTHI+ VG+AM+S E++AY+IMR LDV YVLV+FGG+TGYS+DDINKFLWMVRIGG       + +  YY   GE+RVD+  +P +LNCLMYK+CYYRFG++ TE  +P G+DR R  EIGNKD +L+ LEEA TT++W+VRIYKVK   NR
Sbjct:  519 DAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR 700

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BLAST of FG529054 vs. SwissProt
Match: STT3B_MOUSE (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Mus musculus GN=Stt3b PE=1 SV=2)

HSP 1 Score: 256.529 bits (654), Expect = 7.186e-68
Identity = 119/180 (66.11%), Postives = 141/180 (78.33%), Query Frame = 1

Query:   10 AKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP--VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            A+VMSWWDYGYQI  M NRT +VDNNTWNN+HIA VG+AMSS E  AY IMRSLDVDYVLV+FGGV GYS DDINKFLWMVRI  G  P  + +  Y+   GE+RVDK  +P +LNCLMYK+ YYRFGE+  ++  P G+DR R  EIGNKDIK ++LEEA T+++W+VRIYKVK P NR
Sbjct:  596 ARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRSLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIREGDYFTQQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNR 775

[Close]

BLAST of FG529054 vs. SwissProt
Match: STT3B_HUMAN (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Homo sapiens GN=STT3B PE=1 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 9.385e-68
Identity = 118/180 (65.56%), Postives = 141/180 (78.33%), Query Frame = 1

Query:   10 AKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP--VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            A+VMSWWDYGYQI  M NRT +VDNNTWNN+HIA VG+AMSS E  AY IMR+LDVDYVLV+FGGV GYS DDINKFLWMVRI  G  P  + +  Y+   GE+RVDK  +P +LNCLMYK+ YYRFGE+  ++  P G+DR R  EIGNKDIK ++LEEA T+++W+VRIYKVK P NR
Sbjct:  599 ARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNR 778

[Close]

BLAST of FG529054 vs. SwissProt
Match: STT3_CAEEL (Oligosaccharyl transferase subunit STT3 homolog OS=Caenorhabditis elegans GN=T12A2.2 PE=1 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 4.360e-65
Identity = 119/186 (63.98%), Postives = 137/186 (73.66%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP--VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNRS*VQS 558
            DA+VMSWWDYGYQI  M NRT +VDNNTWNN+HIA VG+AMSS E  AY+IM  LDVDY+LV+FGGV GYS DDINKFLWMVRI  G  P  + +E Y+   GEY    GA+  MLNCLMYK+ YYRFGE    Y +  G+DR RG  IG KDI LEY+EEA TT+NW+VRIYK K   NR  V+S
Sbjct:  541 DARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNESAAYEIMTELDVDYILVIFGGVIGYSGDDINKFLWMVRIAQGEHPKDIREENYFTSTGEYSTGAGASETMLNCLMYKMSYYRFGETRVGYNQAGGFDRTRGYVIGKKDITLEYIEEAYTTENWLVRIYKRKKLPNRPTVKS 726

[Close]

BLAST of FG529054 vs. SwissProt
Match: STT3_SCHPO (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 OS=Schizosaccharomyces pombe GN=stt3 PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 3.241e-52
Identity = 96/178 (53.93%), Postives = 129/178 (72.47%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP--VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPP 534
            D KVMSWWDYGYQI  M +R  +VDNNTWNNTHIATVG+AMSS E++AY I+R  DVDY+L+++GG  GYS+DD+NKFLWM+RI  G++P  +++  ++  NGEYR D  A P M   L+YK+ Y+   +L   +    GYDRAR  ++ +KD +L  +EEA TT + +VR+YKVK P
Sbjct:  544 DTKVMSWWDYGYQIAGMADRITLVDNNTWNNTHIATVGKAMSSPEEKAYPILRKHDVDYILIIYGGTLGYSSDDMNKFLWMIRISQGLWPDEIVERNFFTPNGEYRTDDAATPTMRESLLYKMSYHGAWKL---FPPNQGYDRARNQKLPSKDPQLFTIEEAFTTVHHLVRLYKVKKP 718

[Close]

BLAST of FG529054 vs. SwissProt
Match: STT3_YEAST (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Saccharomyces cerevisiae GN=STT3 PE=1 SV=2)

HSP 1 Score: 187.963 bits (476), Expect = 3.139e-47
Identity = 90/177 (50.85%), Postives = 124/177 (70.06%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFP--VIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDI-KLEYLEEALTTQNWIVRIYKVK 528
            D+KV +WWDYGYQI  M +RT +VDNNTWNNTHIA VG+AM+S E+++Y+I++  DVDYVLV+FGG+ G+  DDINKFLWM+RI  G++P  + +  +Y   GEYRVD  A+  M N L+YK+ Y  F +L   +      DR R   I   D+  L+Y +E  T++NW+VRIY++K
Sbjct:  510 DSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDVDYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETMRNSLLYKMSYKDFPQL---FNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQLK 683

[Close]

BLAST of FG529054 vs. TAIR peptide
Match: AT1G34130.1 (| Symbols: STT3B | staurosporin and temperature sensitive 3-like b | chr1:12430000-12432985 FORWARD LENGTH=735)

HSP 1 Score: 355.14 bits (910), Expect = 1.559e-98
Identity = 166/179 (92.74%), Postives = 174/179 (97.21%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            DAK+MSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYED+AYDIMRSLDV+YVLVVFGGVTGYS+DDINKFLWMVRIGGGVFPVIKEP YLVNGE+RVDKGA+PKMLNCLMYKLCYYRFGELTTEYGKP GYDRARGVEIGNKDIKLE+LEEA TT NWIVRIY+VKPP NR
Sbjct:  556 DAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDDAYDIMRSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEFRVDKGASPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRARGVEIGNKDIKLEHLEEAYTTSNWIVRIYRVKPPTNR 734

[Close]

BLAST of FG529054 vs. TAIR peptide
Match: AT5G19690.1 (| Symbols: STT3A | staurosporin and temperature sensitive 3-like A | chr5:6652649-6658214 FORWARD LENGTH=779)

HSP 1 Score: 248.44 bits (633), Expect = 2.055e-66
Identity = 123/179 (68.72%), Postives = 137/179 (76.54%), Query Frame = 1

Query:    7 DAKVMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYDIMRSLDVDYVLVVFGGVTGYSTDDINKFLWMVRIGGGVFPVIKEPYYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPTGYDRARGVEIGNKDIKLEYLEEALTTQNWIVRIYKVKPPKNR 543
            D KV SWWDYGYQ TAM NRTVIVDNNTWNNTHIATVG AMSS E  A++I  SLDV YVLVVFGG+ GY +DDINKFLWMVRIGGGVFP IKE  YL +G+YR+D  A P MLN LMYKL YYRF E     GK  GYDR R  EIG K  KL + EE  T+ +W+VR+YK+KPP+NR
Sbjct:  579 DDKVASWWDYGYQTTAMANRTVIVDNNTWNNTHIATVGTAMSSPEKAAWEIFNSLDVKYVLVVFGGLIGYPSDDINKFLWMVRIGGGVFPHIKEADYLRDGQYRIDSEATPTMLNSLMYKLSYYRFVETD---GK--GYDRVRRTEIGKKHFKLTHFEEVFTSHHWMVRLYKLKPPRNR 752

The following BLAST results are available for this feature:

BLAST of FG529054 vs. TrEMBL
BLAST of FG529054 vs. SwissProt
BLAST of FG529054 vs. TAIR peptide

BLAST of FG529054 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C5YAH3_SORBI	4.428e-99	93.92	Putative uncharacterized protein Sb06g032800 OS=So...	[more]
C0P8G5_MAIZE	4.428e-99	93.92	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6T514_MAIZE	4.428e-99	93.92	Oligosaccharyl transferase STT3 subunit OS=Zea may...	[more]
Q7XQ88_ORYSJ	2.198e-98	94.44	OSJNBa0018M05.20 protein OS=Oryza sativa subsp. ja...	[more]
Q259H1_ORYSA	2.198e-98	94.44	H0103C06.8 protein OS=Oryza sativa GN=H0103C06.8 P...	[more]
A2XYX2_ORYSI	2.198e-98	94.44	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7TXQ0_VITVI	6.394e-98	95.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
D0ABB6_9ORYZ	8.350e-98	93.89	OO_Ba0005L10-OO_Ba0081K17.25 protein OS=Oryza offi...	[more]
B9N358_POPTR	1.860e-97	94.44	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9I4Z5_POPTR	4.144e-97	93.89	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of FG529054 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
STT3_DICDI	3.682e-72	68.33	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3A_BOVIN	1.184e-70	67.03	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3A_PONAB	3.446e-70	66.48	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3A_MOUSE	3.446e-70	66.48	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3A_HUMAN	3.446e-70	66.48	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3B_MOUSE	7.186e-68	66.11	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3B_HUMAN	9.385e-68	65.56	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3_CAEEL	4.360e-65	63.98	Oligosaccharyl transferase subunit STT3 homolog OS...	[more]
STT3_SCHPO	3.241e-52	53.93	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]
STT3_YEAST	3.139e-47	50.85	Dolichyl-diphosphooligosaccharide--protein glycosy...	[more]

BLAST of FG529054 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G34130.1	1.559e-98	92.74	\| Symbols: STT3B \| staurosporin and temperature se...	[more]
AT5G19690.1	2.055e-66	68.72	\| Symbols: STT3A \| staurosporin and temperature se...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003674	Oligosaccharyl transferase, STT3 subunit	PFAM	PF02516	STT3	coord: 2..132 score: 6.5
None	No IPR available	PANTHER	PTHR13872	60S RIBOSOMAL PROTEIN L35	coord: 2..182 score: 9.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529054 ID=FG529054; Name=FG529054; organism=Pisum sativum; type=EST; length=593bp
CATCCAGATGCCAAGGTGATGTCGTGGTGGGATTATGGTTATCAAATCAC
GGCCATGGGAAACAGAACAGTTATTGTTGACAATAACACCTGGAACAATA
CACACATAGCAACTGTTGGGCGTGCAATGTCATCTTATGAGGATGAGGCT
TATGACATAATGAGATCGCTTGATGTTGACTATGTATTAGTCGTGTTTGG
TGGTGTTACTGGTTATTCCACTGATGATATTAATAAATTTCTATGGATGG
TGAGAATTGGCGGTGGAGTTTTTCCTGTAATAAAGGAACCCTATTACCTT
GTCAATGGAGAGTATCGTGTAGATAAAGGAGCAGCTCCAAAGATGTTGAA
CTGTCTTATGTACAAGCTGTGTTATTATCGGTTTGGGGAGCTGACAACAG
AATATGGCAAACCAACTGGGTATGATCGTGCTAGAGGAGTTGAAATTGGA
AACAAGGACATAAAGCTGGAGTATTTGGAGGAGGCGCTTACTACTCAGAA
TTGGATAGTTCGTATTTATAAAGTGAAACCACCTAAAAACAGGTCGTAAG
TCCAATCTTACCTCCCAAAACAAATCACATAAAAAAGCGTAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004576	oligosaccharyl transferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006486	protein glycosylation

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR003674	Oligo_trans_STT3

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