FG529159

EST Overview


Name	FG529159
Unique Name	FG529159
Type	EST
Organism	Pisum sativum (pea)
Sequence length	582

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG529159 vs. TrEMBL
Match: B9S3N3_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1385680 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 2.996e-72
Identity = 121/147 (82.31%), Postives = 129/147 (87.76%), Query Frame = 1

Query:    7 AIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            AIPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV     A+  + GEMQEGN D VGELSVTLVDARKL Y+F GKTDPYV+LSLGDQ IRSKKNSQTTVIGPPG PIWNQDFHMLV+NP+KQKL IQVKD+LGF DL+IG
Sbjct:  352 AIPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPV-----ANDFRSGEMQEGNSDFVGELSVTLVDARKLSYVFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLYIQVKDSLGFTDLTIG 493

HSP 2 Score: 63.929 bits (154), Expect = 2.996e-72
Identity = 34/46 (73.91%), Postives = 34/46 (73.91%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDLGSLQDTVPTDRIVVLQ            EILLRLTYKAYVEDE
Sbjct:  497 VDLGSLQDTVPTDRIVVLQGGWGVFRKGSSGEILLRLTYKAYVEDE 542

[Close]

BLAST of FG529159 vs. TrEMBL
Match: B9HY18_POPTR (Integral membrane single C2 domain protein OS=Populus trichocarpa GN=NTMC2T5.1 PE=4 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 7.913e-70
Identity = 119/147 (80.95%), Postives = 127/147 (86.39%), Query Frame = 1

Query:    7 AIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            AIPVLSMFL KLLT DLPRLFVRP KIVLDFQKGKAVGPV         + GEMQEGN D VGELSVTLVDARKL Y+F GKTDPYVIL+LGDQI+RSKKNSQTTVIGPPG PIWNQDFHMLV+NP+KQKLNIQVKD+LGF  L+IG
Sbjct:  350 AIPVLSMFLKKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVAN-------ESGEMQEGNRDFVGELSVTLVDARKLSYVFLGKTDPYVILNLGDQIMRSKKNSQTTVIGPPGEPIWNQDFHMLVTNPRKQKLNIQVKDSLGFTGLTIG 489

HSP 2 Score: 61.2326 bits (147), Expect = 7.913e-70
Identity = 32/46 (69.57%), Postives = 34/46 (73.91%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDLGSLQDTVPTD+IVVL+            EILLRLTYKAYVEDE
Sbjct:  493 VDLGSLQDTVPTDKIVVLRGGWGLFRKASSGEILLRLTYKAYVEDE 538

[Close]

BLAST of FG529159 vs. TrEMBL
Match: D7TAD6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_10.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010208001 PE=4 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 3.893e-69
Identity = 118/147 (80.27%), Postives = 125/147 (85.03%), Query Frame = 1

Query:    7 AIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            AIPVLSMFL KLLT DLPRLFVRP K VLDFQKGKAVGPV      +    GEMQEGN D VGELSVTLVDARKL Y+F GKTDPYV LS+GDQ IRSKKNSQTTVIGPPG PIWNQDFHMLV+NP+KQKL IQVKD+LGFADL+IG
Sbjct:  344 AIPVLSMFLKKLLTEDLPRLFVRPKKTVLDFQKGKAVGPV------ENALTGEMQEGNRDFVGELSVTLVDARKLSYVFYGKTDPYVTLSIGDQKIRSKKNSQTTVIGPPGEPIWNQDFHMLVANPRKQKLLIQVKDSLGFADLTIG 484

HSP 2 Score: 62.3882 bits (150), Expect = 3.893e-69
Identity = 33/46 (71.74%), Postives = 34/46 (73.91%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDLGSL+DTVPTDRIVVLQ            EILLRLTYKAYVEDE
Sbjct:  488 VDLGSLKDTVPTDRIVVLQGGWGLFRRGSSGEILLRLTYKAYVEDE 533

[Close]

BLAST of FG529159 vs. TrEMBL
Match: B9HI56_POPTR (Integral membrane single C2 domain protein OS=Populus trichocarpa GN=NTMC2T5.2 PE=4 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 2.497e-68
Identity = 116/147 (78.91%), Postives = 126/147 (85.71%), Query Frame = 1

Query:    7 AIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            AIPVLS+FL KLLT DLPRLFVRP KIVLDFQ GKAVGPV         + GEMQEGN D VGELSVTLVDARKL Y+F GKTDPYVILSLGDQI+RSKKNS+TTVIG PG PIWNQDFHMLV+NP+KQKLNIQVKD+LGF DL++G
Sbjct:  348 AIPVLSLFLKKLLTEDLPRLFVRPKKIVLDFQNGKAVGPVAN-------ESGEMQEGNEDFVGELSVTLVDARKLSYVFFGKTDPYVILSLGDQIMRSKKNSRTTVIGRPGEPIWNQDFHMLVANPRKQKLNIQVKDSLGFTDLTVG 487

HSP 2 Score: 61.2326 bits (147), Expect = 2.497e-68
Identity = 32/46 (69.57%), Postives = 33/46 (71.74%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDLGSLQDTVPTD+IV LQ            EILLRLTYKAYVEDE
Sbjct:  491 VDLGSLQDTVPTDKIVALQGGWGLFRKASSGEILLRLTYKAYVEDE 536

[Close]

BLAST of FG529159 vs. TrEMBL
Match: B6ETT7_ARATH (NTMC2T5.2 protein OS=Arabidopsis thaliana GN=ntmc2T5.2 PE=2 SV=2)

HSP 1 Score: 218.394 bits (555), Expect = 1.220e-64
Identity = 111/146 (76.03%), Postives = 125/146 (85.62%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV+     + +K GEMQEGN D VGELSVTLV+A+KLPY+F G+TDPYVIL +GDQ+IRSKKNSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  357 IPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVS-----EDLKSGEMQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIG 497

HSP 2 Score: 53.1434 bits (126), Expect = 1.220e-64
Identity = 29/46 (63.04%), Postives = 30/46 (65.22%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL SL DTVPTDR V L+            EILLRLTYKAYVEDE
Sbjct:  501 VDLESLPDTVPTDRFVSLRGGWSLFGKGSTGEILLRLTYKAYVEDE 546

[Close]

BLAST of FG529159 vs. TrEMBL
Match: D7LA43_ARALY (Integral membrane single C2 domain protein OS=Arabidopsis lyrata subsp. lyrata GN=NTMC2T5.2 PE=4 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 1.220e-64
Identity = 110/146 (75.34%), Postives = 124/146 (84.93%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV+     + +K GEMQEGN D VGELSVTLV+A+KLPY+F G+TDPYVIL +GDQ+IRSKKNSQTTV G PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  354 IPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVS-----EDLKSGEMQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVFGAPGQPIWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIG 494

HSP 2 Score: 54.6842 bits (130), Expect = 1.220e-64
Identity = 30/46 (65.22%), Postives = 30/46 (65.22%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL SL DTVPTDR V LQ            EILLRLTYKAYVEDE
Sbjct:  498 VDLESLPDTVPTDRFVSLQGGWSLFGKGSTGEILLRLTYKAYVEDE 543

[Close]

BLAST of FG529159 vs. TrEMBL
Match: Q8L626_ARATH (At1g50260 OS=Arabidopsis thaliana GN=At1g50260 PE=2 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 1.221e-64
Identity = 112/146 (76.71%), Postives = 123/146 (84.25%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLTVDLPRLFVRP KIVLDFQKGKAVGPV      + +K GEMQEGN D VGELSVTLVDA+KL Y+F GKTDPY IL LGDQ+IRSK+NSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  345 IPVLSMFLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVL-----EDLKSGEMQEGNKDFVGELSVTLVDAQKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDRLGFADMAIG 485

HSP 2 Score: 54.299 bits (129), Expect = 1.221e-64
Identity = 29/46 (63.04%), Postives = 32/46 (69.57%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL  LQDTVPTDRIVVL+            EILLRLTYK+YVE+E
Sbjct:  489 VDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRLTYKSYVEEE 534

[Close]

BLAST of FG529159 vs. TrEMBL
Match: D7KFX0_ARALY (Integral membrane single C2 domain protein OS=Arabidopsis lyrata subsp. lyrata GN=NTMC2T5.1 PE=4 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 1.020e-63
Identity = 110/146 (75.34%), Postives = 122/146 (83.56%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV+     + +K GEMQEGN D VGELSVTLVDA+ L Y+F GKTDPY IL LGDQ+IRSK+NSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  344 IPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVS-----EDLKSGEMQEGNKDFVGELSVTLVDAQNLRYMFSGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQILQIEVNDRLGFADMAIG 484

HSP 2 Score: 55.0694 bits (131), Expect = 1.020e-63
Identity = 30/46 (65.22%), Postives = 31/46 (67.39%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL  LQDTVPTDRIVVL             EILLRLTYKAYVE+E
Sbjct:  488 VDLRFLQDTVPTDRIVVLHGGWSLFGKGSAGEILLRLTYKAYVEEE 533

[Close]

BLAST of FG529159 vs. TrEMBL
Match: C5XWQ0_SORBI (Putative uncharacterized protein Sb04g038220 OS=Sorghum bicolor GN=Sb04g038220 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.891e-62
Identity = 107/150 (71.33%), Postives = 125/150 (83.33%), Query Frame = 1

Query:    7 AIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVAD---GVKDGEMQEGNMDSVGELSVTLVDARKLPY-LFGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            AIPVLSMFLT+LLT DLPRLFVRP KIVLDFQ+G+A+GPV+    +D    V    +QEGN D VGELSVTLVDARKL + LFGKTDPYV++ L DQ+I+SKKNSQTTVIG PG PIWNQDFHMLV+NP+KQKL IQVKD++G  D++IG
Sbjct:  327 AIPVLSMFLTELLTEDLPRLFVRPKKIVLDFQQGRAMGPVSGSVASDIIQNVASDLIQEGNKDFVGELSVTLVDARKLSFVLFGKTDPYVVMILDDQVIKSKKNSQTTVIGLPGEPIWNQDFHMLVANPRKQKLTIQVKDSIGLTDITIG 476

HSP 2 Score: 55.4546 bits (132), Expect = 1.891e-62
Identity = 28/46 (60.87%), Postives = 32/46 (69.57%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            V+LGSL+DTVPTD+IV L             E+LLRLTYKAYVEDE
Sbjct:  480 VELGSLKDTVPTDKIVTLYGGWGLFGKREAGEVLLRLTYKAYVEDE 525

[Close]

BLAST of FG529159 vs. TrEMBL
Match: Q9SX44_ARATH (F14I3.13 protein OS=Arabidopsis thaliana GN=F14I3.13 PE=4 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 2.058e-61
Identity = 106/140 (75.71%), Postives = 117/140 (83.57%), Query Frame = 1

Query:   28 FLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            FLTKLLTVDLPRLFVRP KIVLDFQKGKAVGPV      + +K GEMQEGN D VGELSVTLVDA+KL Y+F GKTDPY IL LGDQ+IRSK+NSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  339 FLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVL-----EDLKSGEMQEGNKDFVGELSVTLVDAQKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDRLGFADMAIG 473

HSP 2 Score: 54.299 bits (129), Expect = 2.058e-61
Identity = 29/46 (63.04%), Postives = 32/46 (69.57%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL  LQDTVPTDRIVVL+            EILLRLTYK+YVE+E
Sbjct:  481 VDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRLTYKSYVEEE 526

[Close]

BLAST of FG529159 vs. TAIR peptide
Match: AT3G19830.2 (| Symbols: NTMC2TYPE5.2, NTMC2T5.2 | Calcium-dependent lipid-binding (CaLB domain) family protein | chr3:6886338-6889974 REVERSE LENGTH=693)

HSP 1 Score: 218.394 bits (555), Expect = 9.370e-67
Identity = 111/146 (76.03%), Postives = 125/146 (85.62%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV+     + +K GEMQEGN D VGELSVTLV+A+KLPY+F G+TDPYVIL +GDQ+IRSKKNSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  357 IPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVS-----EDLKSGEMQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIG 497

HSP 2 Score: 53.1434 bits (126), Expect = 9.370e-67
Identity = 29/46 (63.04%), Postives = 30/46 (65.22%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL SL DTVPTDR V L+            EILLRLTYKAYVEDE
Sbjct:  501 VDLESLPDTVPTDRFVSLRGGWSLFGKGSTGEILLRLTYKAYVEDE 546

[Close]

BLAST of FG529159 vs. TAIR peptide
Match: AT3G19830.1 (| Symbols: NTMC2TYPE5.2, NTMC2T5.2 | Calcium-dependent lipid-binding (CaLB domain) family protein | chr3:6886338-6889974 REVERSE LENGTH=693)

HSP 1 Score: 218.394 bits (555), Expect = 9.370e-67
Identity = 111/146 (76.03%), Postives = 125/146 (85.62%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLT DLPRLFVRP KIVLDFQKGKAVGPV+     + +K GEMQEGN D VGELSVTLV+A+KLPY+F G+TDPYVIL +GDQ+IRSKKNSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  357 IPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKAVGPVS-----EDLKSGEMQEGNKDFVGELSVTLVNAQKLPYMFSGRTDPYVILRIGDQVIRSKKNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDCLGFADMAIG 497

HSP 2 Score: 53.1434 bits (126), Expect = 9.370e-67
Identity = 29/46 (63.04%), Postives = 30/46 (65.22%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL SL DTVPTDR V L+            EILLRLTYKAYVEDE
Sbjct:  501 VDLESLPDTVPTDRFVSLRGGWSLFGKGSTGEILLRLTYKAYVEDE 546

[Close]

BLAST of FG529159 vs. TAIR peptide
Match: AT1G50260.1 (| Symbols: NTMC2TYPE5.1, NTMC2T5.1 | N-terminal-transmembrane-C2 domain type 5.1 | chr1:18618255-18621643 REVERSE LENGTH=675)

HSP 1 Score: 217.238 bits (552), Expect = 9.371e-67
Identity = 112/146 (76.71%), Postives = 123/146 (84.25%), Query Frame = 1

Query:   10 IPVLSMFLTKLLTVDLPRLFVRPNKIVLDFQKGKAVGPVTVGPVADGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            IPVLSMFLTKLLTVDLPRLFVRP KIVLDFQKGKAVGPV      + +K GEMQEGN D VGELSVTLVDA+KL Y+F GKTDPY IL LGDQ+IRSK+NSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  345 IPVLSMFLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVL-----EDLKSGEMQEGNKDFVGELSVTLVDAQKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDRLGFADMAIG 485

HSP 2 Score: 54.299 bits (129), Expect = 9.371e-67
Identity = 29/46 (63.04%), Postives = 32/46 (69.57%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL  LQDTVPTDRIVVL+            EILLRLTYK+YVE+E
Sbjct:  489 VDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRLTYKSYVEEE 534

[Close]

BLAST of FG529159 vs. TAIR peptide
Match: AT1G50260.2 (| Symbols: NTMC2T5.1 | N-terminal-transmembrane-C2 domain type 5.1 | chr1:18618255-18621643 REVERSE LENGTH=634)

HSP 1 Score: 151.369 bits (381), Expect = 4.609e-47
Identity = 74/101 (73.27%), Postives = 85/101 (84.16%), Query Frame = 1

Query:  145 DGVKDGEMQEGNMDSVGELSVTLVDARKLPYLF-GKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            + +K GEMQEGN D VGELSVTLVDA+KL Y+F GKTDPY IL LGDQ+IRSK+NSQTTVIG PG PIWNQDF  LVSNP++Q L I+V D LGFAD++IG
Sbjct:  344 EDLKSGEMQEGNKDFVGELSVTLVDAQKLRYMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDRLGFADMAIG 444

HSP 2 Score: 54.299 bits (129), Expect = 4.609e-47
Identity = 29/46 (63.04%), Postives = 32/46 (69.57%), Query Frame = 2

Query:  443 VDLGSLQDTVPTDRIVVLQXXXXXXXXXXXXEILLRLTYKAYVEDE 580
            VDL  LQDTVPTDRIVVL+            EILLRLTYK+YVE+E
Sbjct:  448 VDLRFLQDTVPTDRIVVLRGGWSLFGKGSAGEILLRLTYKSYVEEE 493

[Close]

BLAST of FG529159 vs. TAIR peptide
Match: AT1G05500.1 (| Symbols: SYTE, ATSYTE, NTMC2TYPE2.1, NTMC2T2.1, SYT5 | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:1625098-1628940 FORWARD LENGTH=560)

HSP 1 Score: 50.8322 bits (120), Expect = 6.103e-7
Identity = 32/86 (37.21%), Postives = 46/86 (53.49%), Query Frame = 1

Query:  193 GELSVTLVDARKLPY--LFGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPKKQKLNIQVKDALGFADLSIG 444
            G LSVT++ A ++P   L GK DPYV+LS+     +S   S+T V+     P+WNQ F  +V +     L ++V D   F    IG
Sbjct:  435 GVLSVTVISAEEIPIQDLMGKADPYVVLSMK----KSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDYIG 516

The following BLAST results are available for this feature:

BLAST of FG529159 vs. TrEMBL
BLAST of FG529159 vs. TAIR peptide

BLAST of FG529159 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9S3N3_RICCO	2.996e-72	82.31	Putative uncharacterized protein OS=Ricinus commun...	[more]
B9HY18_POPTR	7.913e-70	80.95	Integral membrane single C2 domain protein OS=Popu...	[more]
D7TAD6_VITVI	3.893e-69	80.27	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HI56_POPTR	2.497e-68	78.91	Integral membrane single C2 domain protein OS=Popu...	[more]
B6ETT7_ARATH	1.220e-64	76.03	NTMC2T5.2 protein OS=Arabidopsis thaliana GN=ntmc2...	[more]
D7LA43_ARALY	1.220e-64	75.34	Integral membrane single C2 domain protein OS=Arab...	[more]
Q8L626_ARATH	1.221e-64	76.71	At1g50260 OS=Arabidopsis thaliana GN=At1g50260 PE=...	[more]
D7KFX0_ARALY	1.020e-63	75.34	Integral membrane single C2 domain protein OS=Arab...	[more]
C5XWQ0_SORBI	1.891e-62	71.33	Putative uncharacterized protein Sb04g038220 OS=So...	[more]
Q9SX44_ARATH	2.058e-61	75.71	F14I3.13 protein OS=Arabidopsis thaliana GN=F14I3....	[more]

BLAST of FG529159 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G19830.2	9.370e-67	76.03	\| Symbols: NTMC2TYPE5.2, NTMC2T5.2 \| Calcium-depen...	[more]
AT3G19830.1	9.370e-67	76.03	\| Symbols: NTMC2TYPE5.2, NTMC2T5.2 \| Calcium-depen...	[more]
AT1G50260.1	9.371e-67	76.71	\| Symbols: NTMC2TYPE5.1, NTMC2T5.1 \| N-terminal-tr...	[more]
AT1G50260.2	4.609e-47	73.27	\| Symbols: NTMC2T5.1 \| N-terminal-transmembrane-C2...	[more]
AT1G05500.1	6.103e-7	37.21	\| Symbols: SYTE, ATSYTE, NTMC2TYPE2.1, NTMC2T2.1, ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 calcium-dependent membrane targeting	PFAM	PF00168	C2	coord: 66..146 score: 1
IPR008973	C2 calcium/lipid-binding domain, CaLB	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 64..147 score: 8.2
IPR018029	C2 membrane targeting protein	PROFILE	PS50004	C2	coord: 65..147 score: 10
None	No IPR available	GENE3D	2.60.40.150	no description	coord: 61..141 score: 2.4
None	No IPR available	PANTHER	PTHR10774	CALCIUM-DEPENDENT LIPID-BINDING PROTEIN (CALB RELATED)	coord: 66..137 score: 2.9
None	No IPR available	PANTHER	PTHR10774:SF1	GLUT4 VESICLE PROTEIN-RELATED	coord: 66..137 score: 2.9
None	No IPR available	SEG	seg	seg	coord: 20..36 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529159 ID=FG529159; Name=FG529159; organism=Pisum sativum; type=EST; length=582bp
TGATCAGCAATTCCAGTTCTCTCAATGTTTTTGACCAAACTTCTTACAGT
AGATTTACCTAGACTATTTGTACGGCCAAACAAAATTGTTTTGGACTTCC
AGAAGGGAAAAGCTGTTGGTCCTGTCACTGTTGGTCCCGTCGCTGATGGT
GTTAAAGATGGAGAAATGCAAGAAGGAAACATGGATTCTGTTGGTGAACT
ATCCGTCACTCTTGTGGATGCTAGAAAGCTTCCTTATCTTTTTGGCAAGA
CAGATCCTTATGTTATTCTTAGCCTTGGAGATCAAATCATACGCAGCAAA
AAGAACAGTCAAACTACTGTCATTGGCCCCCCTGGAATGCCAATTTGGAA
TCAGGATTTCCATATGCTTGTTTCCAACCCTAAAAAGCAGAAGTTGAATA
TCCAAGTAAAGGACGCATTGGGATTTGCAGATTTGAGTATAGGTTGATTT
GGGATCTCTTCAAGACACAGTACCAACGGACAGAATTGTGGTCTTACAAG
GGGGTTGGGGTTTTAGAGGAAAGGGCTCATCTGGGGAGATATTGCTTAGG
CTAACATATAAAGCCTATGTGGAAGACGAGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR000008	C2_dom
IPR008973	C2 calcium/lipid-binding domain, CaLB
IPR018029	C2 membrane targeting protein

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