FG529375

EST Overview


Name	FG529375
Unique Name	FG529375
Type	EST
Organism	Pisum sativum (pea)
Sequence length	427

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG529375 vs. TrEMBL
Match: D7SI39_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007909001 PE=4 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 6.005e-70
Identity = 129/141 (91.49%), Postives = 134/141 (95.04%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLHHIEHAAVT+LDRWK+TI   QGKQ LQ PKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAK+IMDRTF DFPWQ+RV VDGVE+EVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM
Sbjct:  477 TVLHHIEHAAVTMLDRWKITILQ-QGKQ-LQAPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKNIMDRTFADFPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 615

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BLAST of FG529375 vs. TrEMBL
Match: A5BDX3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021450 PE=4 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 6.005e-70
Identity = 129/141 (91.49%), Postives = 134/141 (95.04%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLHHIEHAAVT+LDRWK+TI   QGKQ LQ PKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAK+IMDRTF DFPWQ+RV VDGVE+EVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM
Sbjct:  451 TVLHHIEHAAVTMLDRWKITILQ-QGKQ-LQAPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKNIMDRTFADFPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 589

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BLAST of FG529375 vs. TrEMBL
Match: B9I2W0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834296 PE=4 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 1.024e-69
Identity = 127/141 (90.07%), Postives = 133/141 (94.33%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            T+LHHIEHAAVTILDRWKVTI   Q KQ LQPPK+MNNYLG+GCDAKVAL+IHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTF DFPWQ+RV VDGV+IEVPEDAEGVLVANIGSYMGGVDLWQNEDE YDNFDPQSM
Sbjct:  471 TLLHHIEHAAVTILDRWKVTIVKNQRKQ-LQPPKYMNNYLGVGCDAKVALEIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADFPWQVRVEVDGVDIEVPEDAEGVLVANIGSYMGGVDLWQNEDETYDNFDPQSM 610

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BLAST of FG529375 vs. TrEMBL
Match: B9RLR5_RICCO (Diacylglycerol kinase, theta, putative OS=Ricinus communis GN=RCOM_1470420 PE=4 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 1.479e-68
Identity = 125/141 (88.65%), Postives = 132/141 (93.62%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            T+L HIEHAAVTILDRWKV I + QGKQ L  PKFMNNYLG+GCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDRTF DFPWQ+RV VDGVEIEVPEDAEGVL+ANIGSYMGGVDLWQNEDE+YDNFDPQSM
Sbjct:  470 TLLQHIEHAAVTILDRWKVAIVNHQGKQ-LMSPKFMNNYLGVGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRTFADFPWQVRVEVDGVEIEVPEDAEGVLIANIGSYMGGVDLWQNEDESYDNFDPQSM 609

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BLAST of FG529375 vs. TrEMBL
Match: D7M0N5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487625 PE=4 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 1.252e-67
Identity = 120/142 (84.51%), Postives = 136/142 (95.77%), Query Frame = 2

Query:    2 TTVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            +TVL +IEHAAVT+LDRWKV+I + QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSM
Sbjct:  473 STVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSM 613

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BLAST of FG529375 vs. TrEMBL
Match: Q75GS1_ORYSJ (Putative diacylglycerol kinase (With alternative splicing) OS=Oryza sativa subsp. japonica GN=OSJNBa0020H02.13 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 111/141 (78.72%), Postives = 125/141 (88.65%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLH IEHAAVTILDRWKV I   +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+
Sbjct:  485 TVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSI 624

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BLAST of FG529375 vs. TrEMBL
Match: Q10JC6_ORYSJ (Diacylglycerol kinase 1, putative OS=Oryza sativa subsp. japonica GN=LOC_Os03g31180 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 111/141 (78.72%), Postives = 125/141 (88.65%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLH IEHAAVTILDRWKV I   +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+
Sbjct:  408 TVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSI 547

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BLAST of FG529375 vs. TrEMBL
Match: C7IZW2_ORYSJ (Os03g0425300 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os03g0425300 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 111/141 (78.72%), Postives = 125/141 (88.65%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLH IEHAAVTILDRWKV I   +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+
Sbjct:   84 TVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSI 223

[Close]

BLAST of FG529375 vs. TrEMBL
Match: B9F945_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_11338 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 111/141 (78.72%), Postives = 125/141 (88.65%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLH IEHAAVTILDRWKV I   +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+
Sbjct:  408 TVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSI 547

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BLAST of FG529375 vs. TrEMBL
Match: B8AK66_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_12126 PE=4 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.357e-60
Identity = 111/141 (78.72%), Postives = 125/141 (88.65%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            TVLH IEHAAVTILDRWKV I   +GK  L   K+MNNYLGIGCDAKVALDIHNLREENPEKFY+QF+NKVLYAREGAKS++DRTF D PWQ+R+ VDG EIE+PED+EGVLVANI SYMGGVDLW++ED+N DNFDPQS+
Sbjct:  408 TVLHDIEHAAVTILDRWKVAIEDKRGKNVLMV-KYMNNYLGIGCDAKVALDIHNLREENPEKFYSQFLNKVLYAREGAKSMIDRTFVDLPWQVRLEVDGTEIEIPEDSEGVLVANIPSYMGGVDLWKSEDDNPDNFDPQSI 547

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BLAST of FG529375 vs. SwissProt
Match: DGK1_ARATH (Diacylglycerol kinase 1 OS=Arabidopsis thaliana GN=DGK1 PE=1 SV=2)

HSP 1 Score: 258.84 bits (660), Expect = 6.384e-69
Identity = 120/142 (84.51%), Postives = 136/142 (95.77%), Query Frame = 2

Query:    2 TTVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            +TVL +IEHAAVT+LDRWKV+I + QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSM
Sbjct:  473 STVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSM 613

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROMO (Diacylglycerol kinase eta OS=Drosophila mojavensis GN=GI24133 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.302e-18
Identity = 43/105 (40.95%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W N  +  D F P S
Sbjct: 1467 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGNSSKKEDIFLPPS 1570

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKQ_MOUSE (Diacylglycerol kinase theta OS=Mus musculus GN=Dgkq PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.749e-18
Identity = 50/140 (35.71%), Postives = 80/140 (57.14%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTI------SSPQGKQQLQPPKF--MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDEN 400
            +VL  ++ A   ++DRW + +      S+     + +PPK   MNNY GIG DA+++LD H  REE P KF ++F NK +Y R G + I          +IR+ V+  E+E+P   EG++  NI S+  G DLW +++++
Sbjct:  691 SVLVSVDEADAVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGVYVRVGLQKISHS--RSLHKEIRLQVEQQEVELP-SIEGLIFINIPSWGSGADLWGSDNDS 827

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROYA (Diacylglycerol kinase eta OS=Drosophila yakuba GN=GE24946 PE=3 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1459 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1562

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROSI (Diacylglycerol kinase eta OS=Drosophila simulans GN=GD19954 PE=3 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1447 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1550

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROSE (Diacylglycerol kinase eta OS=Drosophila sechellia GN=GM10981 PE=3 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1456 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1559

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROGR (Diacylglycerol kinase eta OS=Drosophila grimshawi GN=GH18973 PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1483 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1586

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROER (Diacylglycerol kinase eta OS=Drosophila erecta GN=GG14241 PE=3 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1460 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1563

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKH_DROAN (Diacylglycerol kinase eta OS=Drosophila ananassae GN=GF18176 PE=3 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 1.492e-17
Identity = 42/105 (40.00%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  110 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQS 424
            MNNY GIG DAK++LD HN REE+PEK  ++  N + Y   G+K ++ +T ++   ++++  DG  I +PE  +G+++ NI S+MGG + W +  +  D F P S
Sbjct: 1458 MNNYFGIGIDAKISLDFHNKREEHPEKCRSRARNYMWYGVLGSKQLLQKTCKNLEQRVQLECDGQRIPLPE-LQGIVILNIPSFMGGTNFWGSSTKKDDIFLPPS 1561

[Close]

BLAST of FG529375 vs. SwissProt
Match: DGKE_MOUSE (Diacylglycerol kinase epsilon OS=Mus musculus GN=Dgke PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 4.342e-17
Identity = 51/135 (37.78%), Postives = 79/135 (58.52%), Query Frame = 2

Query:    8 VLHHIEHAAVTILDRWKVTISSPQGKQQLQPPK--FMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQN-EDENY 403
            VL ++  A    LDRWKV +++ +G   L+ PK   MNNY  +G DA +AL+ H  RE+ P  F ++ +NK +Y   G K  + +  +D   +I + +DG  +E+P + EG++V NIG + GG  LW+   DE Y
Sbjct:  332 VLRNVMEADGIKLDRWKVQVTN-KGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELP-NLEGIIVLNIGYWGGGCRLWEGMGDETY 464

[Close]

BLAST of FG529375 vs. TAIR peptide
Match: AT5G07920.1 (| Symbols: DGK1, ATDGK1 | diacylglycerol kinase1 | chr5:2525197-2528396 REVERSE LENGTH=728)

HSP 1 Score: 258.84 bits (660), Expect = 7.874e-70
Identity = 120/142 (84.51%), Postives = 136/142 (95.77%), Query Frame = 2

Query:    2 TTVLHHIEHAAVTILDRWKVTISSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            +TVL +IEHAAVT+LDRWKV+I + QGKQ LQPPK+MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGA+SIMDRTFEDFPWQ+RV VDGV+IEVPEDAEG+LVANIGSYMGGVDLWQNEDE Y+NFDPQSM
Sbjct:  473 STVLQNIEHAAVTVLDRWKVSILNQQGKQ-LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSM 613

[Close]

BLAST of FG529375 vs. TAIR peptide
Match: AT5G63770.2 (| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 | chr5:25519755-25522404 FORWARD LENGTH=558)

HSP 1 Score: 173.326 bits (438), Expect = 4.351e-44
Identity = 84/143 (58.74%), Postives = 106/143 (74.13%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTI--SSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            T L  I+HAAVT+LDRW V I   S +     +  KFM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR   D PWQ+ + VDG +IE+P+D+EG++V NIGSYMGGVDLWQN+ E+ DNF  Q M
Sbjct:  301 TFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCM 443

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BLAST of FG529375 vs. TAIR peptide
Match: AT5G63770.1 (| Symbols: ATDGK2, DGK2 | diacylglycerol kinase 2 | chr5:25519293-25522404 FORWARD LENGTH=712)

HSP 1 Score: 173.326 bits (438), Expect = 4.351e-44
Identity = 84/143 (58.74%), Postives = 106/143 (74.13%), Query Frame = 2

Query:    5 TVLHHIEHAAVTILDRWKVTI--SSPQGKQQLQPPKFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENYDNFDPQSM 427
            T L  I+HAAVT+LDRW V I   S +     +  KFM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR   D PWQ+ + VDG +IE+P+D+EG++V NIGSYMGGVDLWQN+ E+ DNF  Q M
Sbjct:  455 TFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCM 597

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BLAST of FG529375 vs. TAIR peptide
Match: AT4G30340.1 (| Symbols: ATDGK7, DGK7 | diacylglycerol kinase 7 | chr4:14838465-14840941 REVERSE LENGTH=492)

HSP 1 Score: 49.2914 bits (116), Expect = 9.475e-7
Identity = 46/181 (25.41%), Postives = 65/181 (35.91%), Query Frame = 2

Query:   11 LHHIEHAAVTILDRWKVTISSPQGK------------------QQLQ-----PPKFMN------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLY------------------AREGAKSIMD---RTFEDFPWQIRVVVDGVEIEVPEDAEGVLVANIGSYMGGVDLWQNEDENY 403
            LH     ++  LD WK+ +S P G+                  Q L      PPK  +      NY  IG DA+VA   H+LR E P        NK++Y                  A  G ++IM    +      W+        EI VP+    ++V N+ +Y  G   W N    Y
Sbjct:  226 LHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWE--------EIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKY 398

The following BLAST results are available for this feature:

BLAST of FG529375 vs. TrEMBL
BLAST of FG529375 vs. SwissProt
BLAST of FG529375 vs. TAIR peptide

BLAST of FG529375 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SI39_VITVI	6.005e-70	91.49	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BDX3_VITVI	6.005e-70	91.49	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9I2W0_POPTR	1.024e-69	90.07	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RLR5_RICCO	1.479e-68	88.65	Diacylglycerol kinase, theta, putative OS=Ricinus ...	[more]
D7M0N5_ARALY	1.252e-67	84.51	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q75GS1_ORYSJ	7.357e-60	78.72	Putative diacylglycerol kinase (With alternative s...	[more]
Q10JC6_ORYSJ	7.357e-60	78.72	Diacylglycerol kinase 1, putative OS=Oryza sativa ...	[more]
C7IZW2_ORYSJ	7.357e-60	78.72	Os03g0425300 protein (Fragment) OS=Oryza sativa su...	[more]
B9F945_ORYSJ	7.357e-60	78.72	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AK66_ORYSI	7.357e-60	78.72	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG529375 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DGK1_ARATH	6.384e-69	84.51	Diacylglycerol kinase 1 OS=Arabidopsis thaliana GN...	[more]
DGKH_DROMO	2.302e-18	40.95	Diacylglycerol kinase eta OS=Drosophila mojavensis...	[more]
DGKQ_MOUSE	8.749e-18	35.71	Diacylglycerol kinase theta OS=Mus musculus GN=Dgk...	[more]
DGKH_DROYA	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila yakuba GN=...	[more]
DGKH_DROSI	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila simulans G...	[more]
DGKH_DROSE	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila sechellia ...	[more]
DGKH_DROGR	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila grimshawi ...	[more]
DGKH_DROER	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila erecta GN=...	[more]
DGKH_DROAN	1.492e-17	40.00	Diacylglycerol kinase eta OS=Drosophila ananassae ...	[more]
DGKE_MOUSE	4.342e-17	37.78	Diacylglycerol kinase epsilon OS=Mus musculus GN=D...	[more]

BLAST of FG529375 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G07920.1	7.874e-70	84.51	\| Symbols: DGK1, ATDGK1 \| diacylglycerol kinase1 \|...	[more]
AT5G63770.2	4.351e-44	58.74	\| Symbols: ATDGK2, DGK2 \| diacylglycerol kinase 2 ...	[more]
AT5G63770.1	4.351e-44	58.74	\| Symbols: ATDGK2, DGK2 \| diacylglycerol kinase 2 ...	[more]
AT4G30340.1	9.475e-7	25.41	\| Symbols: ATDGK7, DGK7 \| diacylglycerol kinase 7 ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000756	Diacylglycerol kinase, accessory domain	PFAM	PF00609	DAGK_acc	coord: 36..142 score: 2.1
IPR000756	Diacylglycerol kinase, accessory domain	SMART	SM00045	no description	coord: 36..142 score: 2.2
None	No IPR available	PANTHER	PTHR11255	DIACYLGLYCEROL KINASE	coord: 2..142 score: 1.3
None	No IPR available	PANTHER	PTHR11255:SF8	DIACYLGLYCEROL KINASE, THETA	coord: 2..142 score: 1.3
None	No IPR available	SEG	seg	seg	coord: 8..24 score
None	No IPR available	SEG	seg	seg	coord: 3..15 score
None	No IPR available	SEG	seg	seg	coord: 38..47 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529375 ID=FG529375; Name=FG529375; organism=Pisum sativum; type=EST; length=427bp
TACCACAGTTTTGCATCATATAGAACATGCTGCGGTTACTATTCTTGACC
GGTGGAAGGTAACAATCAGTAGCCCACAAGGAAAGCAACAACTCCAACCC
CCAAAGTTTATGAATAATTATCTTGGAATTGGTTGTGATGCAAAGGTTGC
TCTGGACATCCATAATCTGCGAGAGGAAAATCCAGAGAAGTTTTATAACC
AGTTTATGAATAAAGTTCTATATGCAAGAGAAGGTGCTAAAAGCATAATG
GATAGGACATTTGAGGATTTCCCTTGGCAGATTCGTGTTGTGGTGGATGG
AGTTGAAATTGAGGTCCCTGAGGATGCTGAAGGGGTTCTTGTTGCAAATA
TTGGAAGCTACATGGGAGGTGTAGATTTGTGGCAAAATGAGGATGAGAAT
TATGATAATTTTGATCCACAATCTATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004143	ATP-dependent diacylglycerol kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway

Vocabulary: **INTERPRO**
Term	Definition
IPR000756	Diacylglycerol_kin_accessory

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