FG531284

EST Overview


Name	FG531284
Unique Name	FG531284
Type	EST
Organism	Pisum sativum (pea)
Sequence length	498

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG531284 vs. TrEMBL
Match: C6TAS0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 1.229e-67
Identity = 131/165 (79.39%), Postives = 140/165 (84.85%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTS++QDEVLSHRLGLIPI ADP+LFEYP+NAG + +EKNTIVFKLHV C+ G PRITVKSD LKWLPNGSEL  +  K NA SK KTFTSF+CSQ SLP FS   I      IILAKLGPGQEIELEAHAV
Sbjct:   96 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSVVQDEVLSHRLGLIPIRADPRLFEYPENAGDDKNEKNTIVFKLHVRCQVGQPRITVKSDKLKWLPNGSELPCEGVKPNAGSKPKTFTSFTCSQDSLPEFSNNSIGLTYSDIILAKLGPGQEIELEAHAV 260

[Close]

BLAST of FG531284 vs. TrEMBL
Match: D7UAJ8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_15.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00014713001 PE=4 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.759e-58
Identity = 117/167 (70.06%), Postives = 138/167 (82.63%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSE--LIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGIDP+IANAFRRILIAE+PTMAIE+V IANNTS++QDEVL+HRLGLIPI+ADP+LF+        N EKNTIVF LHV C++G PR+TVKS+ LKWLP+GSE  L  +NT +++ SK KT+TSFSCSQ SLP FS K I P +  II+AKLGPGQEIELEAHAV
Sbjct:   80 DMIGIDPSIANAFRRILIAELPTMAIEKVLIANNTSIVQDEVLAHRLGLIPINADPRLFDDLSENDTPN-EKNTIVFNLHVRCERGGPRLTVKSNELKWLPHGSEFPLATENTTSSSTSKPKTYTSFSCSQDSLPEFSDKSIGPKHPDIIIAKLGPGQEIELEAHAV 245

[Close]

BLAST of FG531284 vs. TrEMBL
Match: Q8LD68_ARATH (RNA polymerase subunit OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 4.533e-54
Identity = 112/165 (67.88%), Postives = 129/165 (78.18%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V+IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVFIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKLKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TrEMBL
Match: B9S9W7_RICCO (DNA-directed RNA polymerase I/III subunits, putative OS=Ricinus communis GN=RCOM_0523110 PE=4 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.010e-53
Identity = 113/165 (68.48%), Postives = 134/165 (81.21%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID AIANAFRRILI+E+PTMAIE+V IANNTSLIQDEVL+HRLGLIPI+ADP+LFEY  +A    +EKNTIVFKLH SCK+G PR TV S+ LKWLP GS+L+ ++ K  ADSK  T+TSF+  Q  +P    KP++P+   II+AKLGPGQEIELEAHAV
Sbjct:   90 DMIGIDAAIANAFRRILISELPTMAIEKVLIANNTSLIQDEVLAHRLGLIPINADPRLFEYL-SANDTPNEKNTIVFKLHASCKRGEPRRTVYSEELKWLPGGSQLLKESEK--ADSKPSTYTSFTDKQDFIP----KPVSPSEDKIIVAKLGPGQEIELEAHAV 247

[Close]

BLAST of FG531284 vs. TrEMBL
Match: Q9C6C2_ARATH (RNA polymerase subunit OS=Arabidopsis thaliana GN=At1g60850 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.319e-53
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TrEMBL
Match: Q3ECL4_ARATH (Putative uncharacterized protein At1g60850.3 OS=Arabidopsis thaliana GN=At1g60850 PE=4 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.319e-53
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TrEMBL
Match: D7KWR8_ARALY (ATRPAC43 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_475268 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 2.938e-53
Identity = 108/165 (65.45%), Postives = 131/165 (79.39%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIG+   IANAFRRIL+AE+P+MAIE+V++ANNTS+IQDEVL+HRLGLIPI ADP+LFEY  +   + +EKNTIVFKLHV C KG PR  V +  LKWLPNGSEL+ ++      +K KT+TSFS SQ S PGF+K PI P+   I++AKLGPGQEIELEAHAV
Sbjct:   96 DMIGVPAGIANAFRRILLAELPSMAIEKVFVANNTSVIQDEVLAHRLGLIPIAADPRLFEYL-SENDQPNEKNTIVFKLHVKCPKGDPRRKVLTSELKWLPNGSELVKES--GGLTTKPKTYTSFSHSQDSFPGFAKNPITPSFQDILIAKLGPGQEIELEAHAV 257

[Close]

BLAST of FG531284 vs. TrEMBL
Match: Q39215_ARATH (RNA polymerase subunit OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.012e-53
Identity = 111/165 (67.27%), Postives = 127/165 (76.97%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKN IVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNNIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TrEMBL
Match: B9GFY9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_550226 PE=4 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.012e-53
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A+ANAFRRILI+EVPTMAIE+V+IANNTS+IQDEVL+HRLGLIPI  DP+LFEY  +A    +EKNTIV KLH  C +G PR +V S  LKWLPNGSE   ++ K   DSK  T+TSFSC Q SLP F+  PI+     IILAKLGPGQEIELEAHAV
Sbjct:  102 DMIGIDAAVANAFRRILISEVPTMAIEKVFIANNTSVIQDEVLAHRLGLIPIKVDPRLFEYL-SANDTPNEKNTIVLKLHARCNRGQPRCSVYSQELKWLPNGSEFAKESEK--PDSKPSTYTSFSCGQDSLPEFAGNPISTVE-DIILAKLGPGQEIELEAHAV 262

[Close]

BLAST of FG531284 vs. TrEMBL
Match: D7KWN9_ARALY (ATRPAC42 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_475246 PE=4 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 3.249e-52
Identity = 108/165 (65.45%), Postives = 129/165 (78.18%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAE+P+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY  +   + +EKNTIVFKLHV C K  PR+ V +  LKWLPNGSE + ++    + SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAELPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYL-SENDQANEKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSEFLRES--GGSTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_HUMAN (DNA-directed RNA polymerases I and III subunit RPAC1 OS=Homo sapiens GN=POLR1C PE=1 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.980e-29
Identity = 77/165 (46.67%), Postives = 106/165 (64.24%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DM+GID AIANAFRRIL+AEVPTMA+E+V + NNTS++QDE+L+HRLGLIPI ADP+LFEY +    E +E +T+ F+L V C +        SD     PN  EL  ++      ++  T+     +Q+ L  F +  I P +  I++A+L PGQEI+L  H V
Sbjct:   64 DMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSD-----PN--ELYVNH---KVYTRHMTWIPLG-NQADL--FPEGTIRPVHDDILIAQLRPGQEIDLLMHCV 215

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_MOUSE (DNA-directed RNA polymerases I and III subunit RPAC1 OS=Mus musculus GN=Polr1e PE=1 SV=2)

HSP 1 Score: 126.331 bits (316), Expect = 7.526e-29
Identity = 72/166 (43.37%), Postives = 102/166 (61.45%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKT-FTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DM+GID AIANAFRRIL+A VPTMA+E+V + NNTS++QDE+L+HRLGLIPI ADP+LFEY +    E +E +T+ F+L V C +               PN ++  +D  +   + K  T   ++    +    F +  I P +  I++A+L PGQEI+L  H V
Sbjct:   64 DMVGIDAAIANAFRRILLARVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPILADPRLFEYRNQGEEEGTEIDTLQFRLQVRCTRN--------------PNAAKDSSDPNELYVNHKVYTRHMTWVPLGNQADVFPEGTIRPVHDDILIAQLRPGQEIDLMMHCV 215

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_BOVIN (DNA-directed RNA polymerases I and III subunit RPAC1 OS=Bos taurus GN=POLR1C PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 9.829e-29
Identity = 76/165 (46.06%), Postives = 105/165 (63.64%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DM+GID AIANAFRRIL+AEVPTMA+E+V + NNTS++QDE+L+HRLGLIPI ADP+LFEY +      +E +T+ F+L V C +        SD     PN  EL  ++      ++  T+     +Q+ L  F +  I P +  I++A+L PGQEI+L  H V
Sbjct:   64 DMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEGGTEIDTLQFRLQVRCTRNPHAAKDSSD-----PN--ELYVNH---RVYTRHMTWVPLG-NQADL--FPEGAIRPVHDDILIAQLRPGQEIDLLMHCV 215

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_SCHPO (DNA-directed RNA polymerases I and III subunit RPAC1 OS=Schizosaccharomyces pombe GN=rpc40 PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 5.393e-27
Identity = 73/169 (43.20%), Postives = 104/169 (61.54%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNA--GGE--NSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            ++ GID +IANAFRRILIAE+PT+A E VYI NNTS+IQDEVLSHR+GL+PI ADP +F++  +   G E  +++ +T+VF L+  C+      T + D  +   N SE+ + +       + +              F+  PI   N  I++AKL PGQEI+LEAHA+
Sbjct:   61 EISGIDASIANAFRRILIAEIPTLAFEFVYIINNTSIIQDEVLSHRIGLVPISADPDMFKWFQHPLPGQEATHTDYDTVVFSLNKKCEFNKNAATDEKDPKRLYVN-SEVYSGDLIWKPQGRQEE------------RFADNPIRVVNPDIVVAKLRPGQEIDLEAHAI 216

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_YEAST (DNA-directed RNA polymerases I and III subunit RPAC1 OS=Saccharomyces cerevisiae GN=RPC40 PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 2.677e-26
Identity = 69/168 (41.07%), Postives = 101/168 (60.12%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGEN---SEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            D+I ID +IANAFRRI+I+EVP++A E VY  NNTS+IQDEVL+HR+GL+P+  DP +  + D+   ++   +++NTIV  L+V C + +P     S   K L N + + A + K     +  T             F+  P+ P +  I+LAKL PGQEI L+AH +
Sbjct:   55 DLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTR-NPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQST------------TFADCPVVPADPDILLAKLRPGQEISLKAHCI 209

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPAC1_DICDI (DNA-directed RNA polymerases I and III subunit rpac1 OS=Dictyostelium discoideum GN=polr1c PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 4.000e-22
Identity = 64/168 (38.10%), Postives = 91/168 (54.17%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHP-----RITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAH 491
            +MIGID  IANA RRI+I+E+PTMAIE+VYI NNTS++QDEVL+HRLG+IPI  DP+ F +      E + ++T++F+L V C+K          T  S  L W+P   +                       +   P    +P  P    I + KL PGQ I+++ +
Sbjct:   74 EMIGIDAPIANALRRIMISEIPTMAIEKVYIINNTSILQDEVLAHRLGMIPIKVDPRKFNF-KLPNKEYTAEDTLIFQLKVKCEKDSVTGKIINETALSKHLIWVPTQDQ-----------------------EEMFPNEEDRP-RPMEDDIPIMKLRPGQVIDIQCY 216

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPB3_DICDI (DNA-directed RNA polymerase II subunit rpb3 OS=Dictyostelium discoideum GN=polr2c PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.290e-12
Identity = 40/113 (35.40%), Postives = 67/113 (59.29%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDAD--PKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKW----LPNGSELIADNTK 338
            D ++ANA RR++IAEVPTM I+ V   +N S++ DE ++HRLGLIP+ +D   K     D +  +  ++ ++  +L+V C +  PR    SD L      +P  S++ + N++
Sbjct:   32 DISVANALRRVMIAEVPTMCIDLVEFESNNSVLCDEFIAHRLGLIPLVSDNIDKFCYTRDCSCSDRCDQCSVELRLNVKCTENRPRDVTSSDLLSQNSAVIPVSSQVTSSNSE 144

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPB3_BOVIN (DNA-directed RNA polymerase II subunit RPB3 OS=Bos taurus GN=POLR2C PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.177e-11
Identity = 51/162 (31.48%), Postives = 75/162 (46.30%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDAD---PKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHA 494
            D A+AN+ RR+ IAEVP +AI+ V I  N+S++ DE ++HRLGLIP+ +D    KL    D    E   + ++ F L V C +   R     D    L + S  +   T  N D+    +                        I++ KL  GQE+ L A+A
Sbjct:   27 DLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRD----LISNSPRVIPVTSRNRDNDPNDYVEQD-------------------DILIVKLRKGQELRLRAYA 165

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPB3_MOUSE (DNA-directed RNA polymerase II subunit RPB3 OS=Mus musculus GN=Polr2c PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 5.418e-11
Identity = 50/161 (31.06%), Postives = 74/161 (45.96%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDAD---PKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAH 491
            D A+AN+ RR+ IAEVP +AI+ V I  N+S++ DE ++HRLGLIP+ +D    KL    D    E   + ++ F L V C +   R     D    L + S  +   T  N D+    +                        I++ KL  GQE+ L A+
Sbjct:   27 DLAVANSIRRVFIAEVPIIAIDWVQIDGNSSVLHDEFIAHRLGLIPLTSDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRD----LISNSPRVIPVTSRNRDNDPNDYVEQD-------------------DILIVKLRKGQELRLRAY 164

[Close]

BLAST of FG531284 vs. SwissProt
Match: RPB3_HUMAN (DNA-directed RNA polymerase II subunit RPB3 OS=Homo sapiens GN=POLR2C PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 5.418e-11
Identity = 51/162 (31.48%), Postives = 75/162 (46.30%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDAD---PKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHA 494
            D A+AN+ RR+ IAEVP +AI+ V I  N+S++ DE ++HRLGLIP+ +D    KL    D    E   + ++ F L V C +   R     D    L + S  +   T  N D+    +                        I++ KL  GQE+ L A+A
Sbjct:   27 DLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRD----LISNSPRVIPVTSRNRDNDPNDYVEQD-------------------DILIVKLRKGQELRLRAYA 165

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT1G60850.1 (| Symbols: ATRPAC42, AAC42 | DNA-directed RNA polymerase family protein | chr1:22398078-22400155 REVERSE LENGTH=375)

HSP 1 Score: 212.231 bits (539), Expect = 1.159e-55
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT1G60850.2 (| Symbols: ATRPAC42, AAC42 | DNA-directed RNA polymerase family protein | chr1:22398078-22400155 REVERSE LENGTH=375)

HSP 1 Score: 212.231 bits (539), Expect = 1.159e-55
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT1G60850.3 (| Symbols: ATRPAC42, AAC42 | DNA-directed RNA polymerase family protein | chr1:22398588-22400155 REVERSE LENGTH=302)

HSP 1 Score: 212.231 bits (539), Expect = 1.159e-55
Identity = 112/165 (67.88%), Postives = 128/165 (77.58%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIGID A ANAFRRILIAEVP+MAIE+V IA NTS+I DEVL+HR+GLIPI ADP+LFEY       N EKNTIVFKLHV C K  PR+ V +  LKWLPNGSEL+ ++   N  SK KT+TSFSCSQ SLP F+  PI P +L I++AKL PGQEIELEAHAV
Sbjct:   89 DMIGIDAAFANAFRRILIAEVPSMAIEKVLIAYNTSVIIDEVLAHRMGLIPIAADPRLFEYLSEHDQAN-EKNTIVFKLHVKCPKNRPRLKVLTSDLKWLPNGSELLRES--ENKTSKPKTYTSFSCSQDSLPEFANNPITPCDLDILIAKLAPGQEIELEAHAV 250

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT1G60620.1 (| Symbols: ATRPAC43, RPAC43 | RNA polymerase I subunit 43 | chr1:22331225-22333370 FORWARD LENGTH=385)

HSP 1 Score: 204.527 bits (519), Expect = 2.417e-53
Identity = 106/165 (64.24%), Postives = 129/165 (78.18%), Query Frame = 3

Query:    3 DMIGIDPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLFEYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHAV 497
            DMIG+   IANAFRRIL+AE+P+MAIE+VY+ANNTS+IQDEVL+HRLGLIPI ADP+LFEY  +   + +EKNTIVFKLHV C KG PR  V +  LKWLPNGSELI ++    + +  KT+TSF+ SQ S P F++ PI P    I++AKLGPGQEIELEAHAV
Sbjct:   96 DMIGVHAGIANAFRRILLAELPSMAIEKVYVANNTSVIQDEVLAHRLGLIPIAADPRLFEYL-SENDQPNEKNTIVFKLHVKCLKGDPRRKVLTSELKWLPNGSELIKES--GGSTTTPKTYTSFNHSQDSFPEFAENPIRPTLKDILIAKLGPGQEIELEAHAV 257

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT2G15400.2 (| Symbols: RBP36B, NRPE3B | DNA-directed RNA polymerase family protein | chr2:6713022-6713918 FORWARD LENGTH=235)

HSP 1 Score: 65.855 bits (159), Expect = 1.342e-11
Identity = 54/164 (32.93%), Postives = 78/164 (47.56%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLF-----EYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHA 494
            D ++ANA RR++I+EVPTMAI  V I  N+S++ DE ++ RL LIP+ ++  +      +  D  G E+ E  ++ F L   C           D            AD T T  D     FTS S +  S            +  II+AKL  GQE++L+A A
Sbjct:   30 DVSMANALRRVMISEVPTMAIHLVKIEVNSSVLNDEFIAQRLSLIPLTSERAMSMRFCQDCEDCNGDEHCEFCSVEFPLSAKCVTDQTLDVTSRDLYS---------ADPTVTPVD-----FTSNSSTSDS----------SEHKGIIIAKLRRGQELKLKALA 169

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT2G15400.1 (| Symbols: RBP36B, NRPE3B | DNA-directed RNA polymerase family protein | chr2:6713022-6714386 FORWARD LENGTH=319)

HSP 1 Score: 65.855 bits (159), Expect = 1.342e-11
Identity = 54/164 (32.93%), Postives = 78/164 (47.56%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKLF-----EYPDNAGGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFTSFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHA 494
            D ++ANA RR++I+EVPTMAI  V I  N+S++ DE ++ RL LIP+ ++  +      +  D  G E+ E  ++ F L   C           D            AD T T  D     FTS S +  S            +  II+AKL  GQE++L+A A
Sbjct:   30 DVSMANALRRVMISEVPTMAIHLVKIEVNSSVLNDEFIAQRLSLIPLTSERAMSMRFCQDCEDCNGDEHCEFCSVEFPLSAKCVTDQTLDVTSRDLYS---------ADPTVTPVD-----FTSNSSTSDS----------SEHKGIIIAKLRRGQELKLKALA 169

[Close]

BLAST of FG531284 vs. TAIR peptide
Match: AT2G15430.1 (| Symbols: RBP36A, RPB35.5A, NRPB3, NRPD3, NRPE3A | DNA-directed RNA polymerase family protein | chr2:6733661-6735482 FORWARD LENGTH=319)

HSP 1 Score: 61.2326 bits (147), Expect = 3.305e-10
Identity = 55/165 (33.33%), Postives = 79/165 (47.88%), Query Frame = 3

Query:   18 DPAIANAFRRILIAEVPTMAIERVYIANNTSLIQDEVLSHRLGLIPIDADPKL---FEYPDNA--GGENSEKNTIVFKLHVSCKKGHPRITVKSDALKWLPNGSELIADNTKTNADSKAKTFT-SFSCSQSSLPGFSKKPIAPNNLXIILAKLGPGQEIELEAHA 494
            D ++ANA RR++I+EVPT+AI+ V I  N+S++ DE ++HRLGLIP+ ++  +   F    +A  G    E  ++ F+L   C           D            AD T T  D     FT   S S SS            +  II+ KL  GQE++L A A
Sbjct:   30 DVSMANALRRVMISEVPTVAIDLVEIEVNSSVLNDEFIAHRLGLIPLTSERAMSMRFSRDCDACDGDGQCEFCSVEFRLSSKCVTDQTLDVTSRDLYS---------ADPTVTPVD-----FTIDSSVSDSS-----------EHKGIIIVKLRRGQELKLRAIA 169

The following BLAST results are available for this feature:

BLAST of FG531284 vs. TrEMBL
BLAST of FG531284 vs. SwissProt
BLAST of FG531284 vs. TAIR peptide

BLAST of FG531284 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TAS0_SOYBN	1.229e-67	79.39	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7UAJ8_VITVI	6.759e-58	70.06	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q8LD68_ARATH	4.533e-54	67.88	RNA polymerase subunit OS=Arabidopsis thaliana PE=...	[more]
B9S9W7_RICCO	1.010e-53	68.48	DNA-directed RNA polymerase I/III subunits, putati...	[more]
Q9C6C2_ARATH	1.319e-53	67.88	RNA polymerase subunit OS=Arabidopsis thaliana GN=...	[more]
Q3ECL4_ARATH	1.319e-53	67.88	Putative uncharacterized protein At1g60850.3 OS=Ar...	[more]
D7KWR8_ARALY	2.938e-53	65.45	ATRPAC43 OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]
Q39215_ARATH	5.012e-53	67.27	RNA polymerase subunit OS=Arabidopsis thaliana PE=...	[more]
B9GFY9_POPTR	5.012e-53	67.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7KWN9_ARALY	3.249e-52	65.45	ATRPAC42 OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]

BLAST of FG531284 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPAC1_HUMAN	1.980e-29	46.67	DNA-directed RNA polymerases I and III subunit RPA...	[more]
RPAC1_MOUSE	7.526e-29	43.37	DNA-directed RNA polymerases I and III subunit RPA...	[more]
RPAC1_BOVIN	9.829e-29	46.06	DNA-directed RNA polymerases I and III subunit RPA...	[more]
RPAC1_SCHPO	5.393e-27	43.20	DNA-directed RNA polymerases I and III subunit RPA...	[more]
RPAC1_YEAST	2.677e-26	41.07	DNA-directed RNA polymerases I and III subunit RPA...	[more]
RPAC1_DICDI	4.000e-22	38.10	DNA-directed RNA polymerases I and III subunit rpa...	[more]
RPB3_DICDI	1.290e-12	35.40	DNA-directed RNA polymerase II subunit rpb3 OS=Dic...	[more]
RPB3_BOVIN	3.177e-11	31.48	DNA-directed RNA polymerase II subunit RPB3 OS=Bos...	[more]
RPB3_MOUSE	5.418e-11	31.06	DNA-directed RNA polymerase II subunit RPB3 OS=Mus...	[more]
RPB3_HUMAN	5.418e-11	31.48	DNA-directed RNA polymerase II subunit RPB3 OS=Hom...	[more]

BLAST of FG531284 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G60850.1	1.159e-55	67.88	\| Symbols: ATRPAC42, AAC42 \| DNA-directed RNA poly...	[more]
AT1G60850.2	1.159e-55	67.88	\| Symbols: ATRPAC42, AAC42 \| DNA-directed RNA poly...	[more]
AT1G60850.3	1.159e-55	67.88	\| Symbols: ATRPAC42, AAC42 \| DNA-directed RNA poly...	[more]
AT1G60620.1	2.417e-53	64.24	\| Symbols: ATRPAC43, RPAC43 \| RNA polymerase I sub...	[more]
AT2G15400.2	1.342e-11	32.93	\| Symbols: RBP36B, NRPE3B \| DNA-directed RNA polym...	[more]
AT2G15400.1	1.342e-11	32.93	\| Symbols: RBP36B, NRPE3B \| DNA-directed RNA polym...	[more]
AT2G15430.1	3.305e-10	33.33	\| Symbols: RBP36A, RPB35.5A, NRPB3, NRPD3, NRPE3A ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001514	DNA-directed RNA polymerase, 30-40kDa subunit, conserved site	PROSITE	PS00446	RNA_POL_D_30KD	coord: 11..51 score
IPR009025	DNA-directed RNA polymerase, RBP11-like	SUPERFAMILY	55257	RBP11-like subunits of RNA polymerase	coord: 1..27 score: 4
IPR011261	DNA-directed RNA polymerase, dimerisation	PFAM	PF01193	RNA_pol_L	coord: 3..78 score: 2.9
IPR011262	DNA-directed RNA polymerase, insert domain	GENE3D	2.170.120.12	no description	coord: 23..164 score: 2.8
IPR011262	DNA-directed RNA polymerase, insert domain	PFAM	PF01000	RNA_pol_A_bac	coord: 29..164 score: 3.1
IPR011262	DNA-directed RNA polymerase, insert domain	SUPERFAMILY	56553	Insert subdomain of RNA polymerase alpha subunit	coord: 22..165 score: 3.2
IPR011263	DNA-directed RNA polymerase, RpoA/D/Rpb3-type	SMART	SM00662	no description	coord: 1..165 score: 2.8
None	No IPR available	GENE3D	3.30.1360.10	no description	coord: 1..22 score: 3
None	No IPR available	PANTHER	PTHR11800	DNA-DIRECTED RNA POLYMERASE	coord: 1..165 score: 3.8
None	No IPR available	PANTHER	PTHR11800:SF13	DNA-DIRECTED RNA POLYMERASE I	coord: 1..165 score: 3.8
None	No IPR available	SEG	seg	seg	coord: 61..72 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531284 ID=FG531284; Name=FG531284; organism=Pisum sativum; type=EST; length=498bp
TTGACATGATTGGTATTGATCCAGCAATTGCCAATGCTTTTCGAAGAATT
CTTATAGCAGAGGTTCCAACAATGGCTATTGAAAGAGTATATATTGCGAA
CAATACATCACTTATACAAGATGAAGTTCTTTCCCATAGATTGGGTCTCA
TTCCAATTGATGCTGATCCCAAGCTATTTGAATATCCAGACAATGCGGGA
GGTGAAAACAGTGAAAAGAACACCATCGTTTTCAAACTTCATGTTAGTTG
TAAAAAGGGTCATCCCCGTATTACCGTGAAATCAGATGCACTGAAGTGGT
TGCCTAACGGAAGTGAGCTTATCGCTGATAATACCAAAACAAATGCCGAT
TCAAAGGCAAAAACATTTACATCTTTTAGTTGCAGTCAAAGCTCACTTCC
TGGATTCTCCAAAAAACCAATTGCTCCTAATAATTTGNATATTATATTAG
CTAAACTTGGACCTGGTCAGGAAATTGAACTTGAAGCTCATGCTGTTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0046983	protein dimerization activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription

Vocabulary: **INTERPRO**
Term	Definition
IPR001514	DNA-dir_RNA_pol_30-40kDasu_CS
IPR009025	RBP11-like_dimer
IPR011261	DNA-directed RNA polymerase, dimerisation
IPR011262	DNA-dir_RNA_pol_insert
IPR011263	DNA-dir_RNA_pol_RpoA/D/Rpb3

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