FG531745

EST Overview


Name	FG531745
Unique Name	FG531745
Type	EST
Organism	Pisum sativum (pea)
Sequence length	425

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG531745 vs. TrEMBL
Match: D7SJT2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024680001 PE=4 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 3.779e-72
Identity = 130/141 (92.20%), Postives = 138/141 (97.87%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRMNPIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHK+EDYRNTFANLALPLFSMAEPVPPKVI+H+++SWTVWDRW L +NPTLRE+LQWLK KGLNAYS
Sbjct:  839 FRMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKLEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWILKDNPTLRELLQWLKDKGLNAYS 979

[Close]

BLAST of FG531745 vs. TrEMBL
Match: B9HNB0_POPTR (Ubiquitin-activating enzyme E1 OS=Populus trichocarpa GN=POPTRDRAFT_722604 PE=3 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 4.935e-72
Identity = 130/141 (92.20%), Postives = 137/141 (97.16%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRM PIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVI+HQ++SWTVWDRW L NNPTLRE++QWLK KGL+AYS
Sbjct:  811 FRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHQDMSWTVWDRWILKNNPTLRELMQWLKDKGLDAYS 951

[Close]

BLAST of FG531745 vs. TrEMBL
Match: B9SKZ1_RICCO (Ubiquitin-activating enzyme E1 OS=Ricinus communis GN=RCOM_0847580 PE=3 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 8.418e-72
Identity = 129/141 (91.49%), Postives = 137/141 (97.16%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRMNPIQFEKDDDTNYHMD+IAGLANMRARNY IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRN+FANLALPLFSMAEPVPPKVI+HQ++SWTVWDRW L NNPTLRE+L+WL+ KGLNAYS
Sbjct:  893 FRMNPIQFEKDDDTNYHMDLIAGLANMRARNYGIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNSFANLALPLFSMAEPVPPKVIKHQDMSWTVWDRWILRNNPTLRELLEWLQHKGLNAYS 1033

[Close]

BLAST of FG531745 vs. TrEMBL
Match: Q75VJ9_TOBAC (Ubiquitin-activating enzyme E1 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.449e-71
Identity = 127/141 (90.07%), Postives = 138/141 (97.87%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            ++MNPIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKV++HQ+++WTVWDRW L +NPTLRE+LQWL+ KGLNAYS
Sbjct:  873 YKMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDMNWTVWDRWILKDNPTLRELLQWLQNKGLNAYS 1013

[Close]

BLAST of FG531745 vs. TrEMBL
Match: Q75VJ8_TOBAC (Ubiquitin-activating enzyme E1 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.449e-71
Identity = 127/141 (90.07%), Postives = 138/141 (97.87%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            ++MNPIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKV++HQ+++WTVWDRW L +NPTLRE+LQWL+ KGLNAYS
Sbjct:  873 YKMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDMNWTVWDRWILKDNPTLRELLQWLQNKGLNAYS 1013

[Close]

BLAST of FG531745 vs. TrEMBL
Match: P93393_TOBAC (Ubiquitin-activating enzyme E1 OS=Nicotiana tabacum GN=UBA1 PE=2 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.449e-71
Identity = 127/141 (90.07%), Postives = 138/141 (97.87%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            ++MNPIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKV++HQ+++WTVWDRW L +NPTLRE+LQWL+ KGLNAYS
Sbjct:  873 YKMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDMNWTVWDRWILKDNPTLRELLQWLQNKGLNAYS 1013

[Close]

BLAST of FG531745 vs. TrEMBL
Match: B9GGR5_POPTR (Ubiquitin-activating enzyme E1 OS=Populus trichocarpa GN=POPTRDRAFT_815464 PE=3 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.449e-71
Identity = 129/141 (91.49%), Postives = 137/141 (97.16%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            +RM PIQFEKDDDTNYHMD+IAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVI+HQ++SWTVWDRW L +NPTLRE+LQWLK KGL+AYS
Sbjct:  811 YRMTPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHQDMSWTVWDRWILKDNPTLRELLQWLKHKGLDAYS 951

[Close]

BLAST of FG531745 vs. TrEMBL
Match: D7LBJ8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_481921 PE=4 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 1.216e-70
Identity = 127/141 (90.07%), Postives = 136/141 (96.45%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRM PIQFEKDDDTNYHMDVIAGLANMRARNY+IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKV++H++++WTVWDRW L  NPTLREVLQWL+ KGL+AYS
Sbjct:  876 FRMKPIQFEKDDDTNYHMDVIAGLANMRARNYTIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1016

[Close]

BLAST of FG531745 vs. TrEMBL
Match: D7SQU6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_58.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030304001 PE=4 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.588e-70
Identity = 126/141 (89.36%), Postives = 137/141 (97.16%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRMNPIQFEKDDD+NYHMD+I+ LANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL GGHK+EDY+NTFANLALPLFSMAEPVPPKVI+HQ++SWTVWDRW LG+NPTLRE+LQWL+ KGLNAYS
Sbjct:  811 FRMNPIQFEKDDDSNYHMDLISALANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLHGGHKMEDYKNTFANLALPLFSMAEPVPPKVIKHQDMSWTVWDRWILGDNPTLRELLQWLRDKGLNAYS 951

[Close]

BLAST of FG531745 vs. TrEMBL
Match: D7LZQ7_ARALY (ATUBA2 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487455 PE=4 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 4.619e-70
Identity = 126/141 (89.36%), Postives = 133/141 (94.33%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRM PIQFEKDDDTNYHMD+IAGLANMRARNYS+PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDG HKVEDYRNTFANLALPLFSMAEPVPPKV++HQ+ SWTVWDRW +  NPTLRE+L WLK KGLNAYS
Sbjct:  872 FRMKPIQFEKDDDTNYHMDMIAGLANMRARNYSVPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGSHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDQSWTVWDRWVIRGNPTLRELLGWLKGKGLNAYS 1012

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBE12_WHEAT (Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum GN=UBA2 PE=2 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 5.618e-70
Identity = 125/141 (88.65%), Postives = 132/141 (93.62%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            F MNPIQFEKDDDTN+HMDVIAG ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFANLA+PLFS+AEPVPPK I+HQELSWTVWDRWT+  N TLRE+L+WLK KGLNAYS
Sbjct:  844 FHMNPIQFEKDDDTNFHMDVIAGFANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYS 984

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBE11_WHEAT (Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum GN=UBA1 PE=1 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 5.618e-70
Identity = 125/141 (88.65%), Postives = 132/141 (93.62%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            F MNPIQFEKDDDTN+HMDVIAG ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRNTFANLA+PLFS+AEPVPPK I+HQELSWTVWDRWT+  N TLRE+L+WLK KGLNAYS
Sbjct:  844 FHMNPIQFEKDDDTNFHMDVIAGFANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRNTFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYS 984

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBE13_WHEAT (Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum GN=UBA3 PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 9.282e-65
Identity = 117/141 (82.98%), Postives = 128/141 (90.78%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            F+M PIQFEKDDDTN+HMD+I+GLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKV+ G H VEDYRNTFANLALPLFSMAEPVPPKV++H+E SWTVWDRW++  N TL E+LQW   KGL AYS
Sbjct:  846 FQMKPIQFEKDDDTNFHMDLISGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVIAGEHPVEDYRNTFANLALPLFSMAEPVPPKVMKHKETSWTVWDRWSVQGNLTLAELLQWFADKGLTAYS 986

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1Y_MOUSE (Ubiquitin-like modifier-activating enzyme 1 Y OS=Mus musculus GN=Ube1ay PE=2 SV=2)

HSP 1 Score: 150.214 bits (378), Expect = 3.111e-36
Identity = 70/141 (49.65%), Postives = 98/141 (69.50%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDD+N+HMD I   +N+RA NY I   D+ K+K IAG+IIPAIAT+T+   GLVCLELYKV+ G  ++E Y+N+F NLALPLFS + P+ P+  ++ +  WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDSNFHMDFIVAASNLRAENYGISPADRHKSKLIAGKIIPAIATTTSAIVGLVCLELYKVVQGHQQLESYKNSFINLALPLFSFSAPLAPECHQYYDQEWTLWDRFDVQGLQPSGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_BOVIN (Ubiquitin-like modifier-activating enzyme 1 OS=Bos taurus GN=UBA1 PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 2.634e-35
Identity = 70/141 (49.65%), Postives = 93/141 (65.96%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDDTN+HMD I   +N+RA NY IP  D+ K+K IAG+IIPAIAT+TA   GLVCLELYKV+ G  ++  Y+N F NLALP F  +EP+     ++    WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_HUMAN (Ubiquitin-like modifier-activating enzyme 1 OS=Homo sapiens GN=UBA1 PE=1 SV=3)

HSP 1 Score: 146.747 bits (369), Expect = 3.440e-35
Identity = 69/141 (48.94%), Postives = 94/141 (66.67%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDD+N+HMD I   +N+RA NY IP  D+ K+K IAG+IIPAIAT+TA   GLVCLELYKV+ G  +++ Y+N F NLALP F  +EP+     ++    WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_RABIT (Ubiquitin-like modifier-activating enzyme 1 OS=Oryctolagus cuniculus GN=UBA1 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.664e-35
Identity = 69/141 (48.94%), Postives = 93/141 (65.96%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDD+N+HMD I   +N+RA NY IP  D+ K+K IAG+IIPAIAT+TA   GLVCLELYKV+ G   ++ Y+N F NLALP F  +EP+     ++    WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRHLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_RAT (Ubiquitin-like modifier-activating enzyme 1 OS=Rattus norvegicus GN=Uba1 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 3.803e-34
Identity = 68/141 (48.23%), Postives = 93/141 (65.96%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDD+N+HMD I   +N+RA NY I   D+ K+K IAG+IIPAIAT+TA   GLVCLELYKV+ G  +++ Y+N F NLALP F  +EP+     ++    WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_MOUSE (Ubiquitin-like modifier-activating enzyme 1 OS=Mus musculus GN=Uba1 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 3.803e-34
Identity = 68/141 (48.23%), Postives = 93/141 (65.96%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTL------GNNPTLREVLQWLKAK 407
            F+M PI FEKDDD+N+HMD I   +N+RA NY I   D+ K+K IAG+IIPAIAT+TA   GLVCLELYKV+ G  +++ Y+N F NLALP F  +EP+     ++    WT+WDR+ +      G   TL++ L + K +
Sbjct:  842 FKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTE 982

[Close]

BLAST of FG531745 vs. SwissProt
Match: UBA1_SCHPO (Ubiquitin-activating enzyme E1 1 OS=Schizosaccharomyces pombe GN=ptr3 PE=1 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 2.307e-31
Identity = 65/140 (46.43%), Postives = 100/140 (71.43%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPV--PPKVIEHQELSWTVWDRWTLGNNPTLREVLQWL-KAKGL 413
            FR+ P +FEKDDD+N+H+D I   +N+RA NY I   D+ K KF+AG+I+PA+ TSTA+ +GLVCLEL K++DG  K+E+Y+N F NLA+ LF+ ++P+  P   +  +E+   +WDR+ L  + TL+E++ +  K +GL
Sbjct:  803 FRLTPAEFEKDDDSNHHIDFITAASNLRAMNYDITPADRFKTKFVAGKIVPAMCTSTAVVSGLVCLELVKLVDGKKKIEEYKNGFFNLAIGLFTFSDPIASPKMKVNGKEID-KIWDRYNL-PDCTLQELIDYFQKEEGL 940

[Close]

BLAST of FG531745 vs. TAIR peptide
Match: AT2G30110.1 (| Symbols: ATUBA1, MOS5, UBA1 | ubiquitin-activating enzyme 1 | chr2:12852632-12857369 REVERSE LENGTH=1080)

HSP 1 Score: 265.774 bits (678), Expect = 6.317e-72
Identity = 126/141 (89.36%), Postives = 135/141 (95.74%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRM PIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE YRNTFANLALPLFSMAEP+PPKV++H++++WTVWDRW L  NPTLREVLQWL+ KGL+AYS
Sbjct:  873 FRMKPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAYRNTFANLALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYS 1013

[Close]

BLAST of FG531745 vs. TAIR peptide
Match: AT5G06460.1 (| Symbols: ATUBA2, UBA 2 | ubiquitin activating enzyme 2 | chr5:1970239-1974382 FORWARD LENGTH=1077)

HSP 1 Score: 261.922 bits (668), Expect = 9.122e-71
Identity = 123/141 (87.23%), Postives = 131/141 (92.91%), Query Frame = 3

Query:    3 FRMNPIQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIEHQELSWTVWDRWTLGNNPTLREVLQWLKAKGLNAYS 425
            FRM  IQFEKDDDTNYHMD+IAGLANMRARNYS+PEVDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPLFSMAEPVPPKV++HQ+ SWTVWDRW +  NPTLRE+L WLK KGLNAYS
Sbjct:  870 FRMKAIQFEKDDDTNYHMDMIAGLANMRARNYSVPEVDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYS 1010

[Close]

BLAST of FG531745 vs. TAIR peptide
Match: AT2G21470.3 (| Symbols: SAE2 | SUMO-activating enzyme 2 | chr2:9198752-9202136 FORWARD LENGTH=628)

HSP 1 Score: 51.2174 bits (121), Expect = 2.445e-7
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 3

Query:   18 IQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTF 257
            + F+KDD     ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+
Sbjct:  337 LTFDKDDQLA--VEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTY 412

[Close]

BLAST of FG531745 vs. TAIR peptide
Match: AT2G21470.2 (| Symbols: SAE2, ATSAE2, EMB2764 | SUMO-activating enzyme 2 | chr2:9198752-9202136 FORWARD LENGTH=700)

HSP 1 Score: 51.2174 bits (121), Expect = 2.445e-7
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 3

Query:   18 IQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTF 257
            + F+KDD     ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+
Sbjct:  334 LTFDKDDQLA--VEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTY 409

[Close]

BLAST of FG531745 vs. TAIR peptide
Match: AT2G21470.1 (| Symbols: SAE2, ATSAE2, EMB2764 | SUMO-activating enzyme 2 | chr2:9198752-9202136 FORWARD LENGTH=625)

HSP 1 Score: 51.2174 bits (121), Expect = 2.445e-7
Identity = 29/80 (36.25%), Postives = 47/80 (58.75%), Query Frame = 3

Query:   18 IQFEKDDDTNYHMDVIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTF 257
            + F+KDD     ++ +   AN+RA ++ IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+
Sbjct:  334 LTFDKDDQLA--VEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTY 409

The following BLAST results are available for this feature:

BLAST of FG531745 vs. TrEMBL
BLAST of FG531745 vs. SwissProt
BLAST of FG531745 vs. TAIR peptide

BLAST of FG531745 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SJT2_VITVI	3.779e-72	92.20	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HNB0_POPTR	4.935e-72	92.20	Ubiquitin-activating enzyme E1 OS=Populus trichoca...	[more]
B9SKZ1_RICCO	8.418e-72	91.49	Ubiquitin-activating enzyme E1 OS=Ricinus communis...	[more]
Q75VJ9_TOBAC	2.449e-71	90.07	Ubiquitin-activating enzyme E1 OS=Nicotiana tabacu...	[more]
Q75VJ8_TOBAC	2.449e-71	90.07	Ubiquitin-activating enzyme E1 OS=Nicotiana tabacu...	[more]
P93393_TOBAC	2.449e-71	90.07	Ubiquitin-activating enzyme E1 OS=Nicotiana tabacu...	[more]
B9GGR5_POPTR	2.449e-71	91.49	Ubiquitin-activating enzyme E1 OS=Populus trichoca...	[more]
D7LBJ8_ARALY	1.216e-70	90.07	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7SQU6_VITVI	1.588e-70	89.36	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LZQ7_ARALY	4.619e-70	89.36	ATUBA2 OS=Arabidopsis lyrata subsp. lyrata GN=ARAL...	[more]

BLAST of FG531745 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
UBE12_WHEAT	5.618e-70	88.65	Ubiquitin-activating enzyme E1 2 OS=Triticum aesti...	[more]
UBE11_WHEAT	5.618e-70	88.65	Ubiquitin-activating enzyme E1 1 OS=Triticum aesti...	[more]
UBE13_WHEAT	9.282e-65	82.98	Ubiquitin-activating enzyme E1 3 OS=Triticum aesti...	[more]
UBA1Y_MOUSE	3.111e-36	49.65	Ubiquitin-like modifier-activating enzyme 1 Y OS=M...	[more]
UBA1_BOVIN	2.634e-35	49.65	Ubiquitin-like modifier-activating enzyme 1 OS=Bos...	[more]
UBA1_HUMAN	3.440e-35	48.94	Ubiquitin-like modifier-activating enzyme 1 OS=Hom...	[more]
UBA1_RABIT	7.664e-35	48.94	Ubiquitin-like modifier-activating enzyme 1 OS=Ory...	[more]
UBA1_RAT	3.803e-34	48.23	Ubiquitin-like modifier-activating enzyme 1 OS=Rat...	[more]
UBA1_MOUSE	3.803e-34	48.23	Ubiquitin-like modifier-activating enzyme 1 OS=Mus...	[more]
UBA1_SCHPO	2.307e-31	46.43	Ubiquitin-activating enzyme E1 1 OS=Schizosaccharo...	[more]

BLAST of FG531745 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G30110.1	6.317e-72	89.36	\| Symbols: ATUBA1, MOS5, UBA1 \| ubiquitin-activati...	[more]
AT5G06460.1	9.122e-71	87.23	\| Symbols: ATUBA2, UBA 2 \| ubiquitin activating en...	[more]
AT2G21470.3	2.445e-7	36.25	\| Symbols: SAE2 \| SUMO-activating enzyme 2 \| chr2:...	[more]
AT2G21470.2	2.445e-7	36.25	\| Symbols: SAE2, ATSAE2, EMB2764 \| SUMO-activating...	[more]
AT2G21470.1	2.445e-7	36.25	\| Symbols: SAE2, ATSAE2, EMB2764 \| SUMO-activating...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 13..36 score
None	No IPR available	PANTHER	PTHR10953	UBIQUITIN-ACTIVATING ENZYME E1	coord: 12..72 score: 9.8
None	No IPR available	PANTHER	PTHR10953:SF6	UBIQUITIN-ACTIVATING ENZYME E1C	coord: 12..72 score: 9.8
IPR000127	Ubiquitin-activating enzyme repeat	PFAM	PF02134	UBACT	coord: 7..74 score: 2.5
IPR009036	Molybdenum cofactor biosynthesis, MoeB	SUPERFAMILY	69572	Activating enzymes of the ubiquitin-like proteins	coord: 1..135 score: 7.6
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 4..101 score: 6
IPR018965	Ubiquitin-activating enzyme e1, C-terminal	PFAM	PF09358	UBA_e1_C	coord: 81..135 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG531745 ID=FG531745; Name=FG531745; organism=Pisum sativum; type=EST; length=425bp
AATTCAGGATGAATCCAATTCAGTTTGAGAAGGATGATGATACAAACTAC
CATATGGATGTGATTGCTGGGCTTGCAAATATGAGGGCTCGGAATTACAG
CATTCCTGAGGTTGACAAGCTTAAAGCCAAATTTATTGCTGGACGAATTA
TTCCTGCTATTGCAACCTCAACTGCCATGGCCACTGGTCTTGTCTGCCTG
GAGTTGTACAAAGTTTTGGATGGGGGCCACAAAGTTGAAGACTATAGAAA
TACATTTGCAAACTTAGCATTGCCTCTATTTTCAATGGCCGAGCCAGTCC
CACCAAAGGTTATTGAACATCAAGAGTTGAGTTGGACAGTTTGGGACAGA
TGGACACTGGGAAACAACCCTACCCTGAGGGAAGTTCTTCAGTGGCTGAA
GGCTAAAGGCTTGAATGCTTATAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0008641	ubiquitin-like modifier activating enzyme activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006464	cellular protein modification process
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000127	Ubiquitin-activating enzyme repeat
IPR009036	Molybdenum cofactor biosynthesis, MoeB
IPR016040	NAD(P)-bd_dom
IPR018965	Ub-activating_enz_E1_C

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