FG532124

EST Overview


Name	FG532124
Unique Name	FG532124
Type	EST
Organism	Pisum sativum (pea)
Sequence length	409

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG532124 vs. TrEMBL
Match: B7FM35_MEDTR (Homoserine dehydrogenase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.862e-63
Identity = 121/136 (88.97%), Postives = 131/136 (96.32%), Query Frame = 1

Query:    1 GQIVEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            G+I+EIASQLGKK+GLAFVDCTASS T+ VLKQVVD+GCCVVMANKKPLTSTM D+E L+ YPRRIRHESTVGAGLPVI+SLNRIISSGDPV+RI+GSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD
Sbjct:   95 GKILEIASQLGKKTGLAFVDCTASSDTIVVLKQVVDLGCCVVMANKKPLTSTMGDFEKLLTYPRRIRHESTVGAGLPVISSLNRIISSGDPVNRIIGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: Q3S3F6_SOYBN (Homoserine dehydrogenase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 1.743e-61
Identity = 117/136 (86.03%), Postives = 127/136 (93.38%), Query Frame = 1

Query:    1 GQIVEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            G+I+EIA QLGKK+GL FVDC+ASS TV+VLKQ +DMGCC VMANKKPLTSTMED++ L  YPRRIRHESTVGAGLPVIASLNRIISSGDPVH+I+GSLSGTLGYVMSEVEDGKPLSQVVR AKSLGYTEPDPRDD
Sbjct:   95 GKILEIAFQLGKKTGLVFVDCSASSDTVAVLKQAIDMGCCAVMANKKPLTSTMEDFKKLFIYPRRIRHESTVGAGLPVIASLNRIISSGDPVHQIIGSLSGTLGYVMSEVEDGKPLSQVVRDAKSLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: C6TCU7_SOYBN (Homoserine dehydrogenase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 1.130e-60
Identity = 116/136 (85.29%), Postives = 126/136 (92.65%), Query Frame = 1

Query:    1 GQIVEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            G+I+EIA QLGKK+GL FVDC+ASS TV+VLKQ + MGCC VMANKKPLTSTMED++ L  YPRRIRHESTVGAGLPVIASLNRIISSGDPVH+I+GSLSGTLGYVMSEVEDGKPLSQVVR AKSLGYTEPDPRDD
Sbjct:   95 GKILEIAFQLGKKTGLVFVDCSASSDTVAVLKQAIGMGCCAVMANKKPLTSTMEDFKKLFIYPRRIRHESTVGAGLPVIASLNRIISSGDPVHQIIGSLSGTLGYVMSEVEDGKPLSQVVRDAKSLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: B9MYR6_POPTR (Homoserine dehydrogenase OS=Populus trichocarpa GN=POPTRDRAFT_594921 PE=3 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 3.077e-58
Identity = 107/135 (79.26%), Postives = 126/135 (93.33%), Query Frame = 1

Query:    4 QIVEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            ++++IA+ LGK +GLAFVDC+ASS T+ +L QVVDMGCCVV+ANKKPLTSTMEDY+ L+ YPRRIRHESTVGAGLPVIASLNR++SSGDPVHRI+GSLSGTLGYVMSEVEDGKP S+VV+ AK+LG+TEPDPRDD
Sbjct:   96 KVMDIAALLGKATGLAFVDCSASSETIRMLNQVVDMGCCVVLANKKPLTSTMEDYDKLVSYPRRIRHESTVGAGLPVIASLNRLLSSGDPVHRIIGSLSGTLGYVMSEVEDGKPFSEVVKVAKNLGFTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: B9S6K6_RICCO (Homoserine dehydrogenase OS=Ricinus communis GN=RCOM_1108890 PE=3 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 1.994e-57
Identity = 105/133 (78.95%), Postives = 125/133 (93.98%), Query Frame = 1

Query:   10 VEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            ++IA+ LGK +GLAFVDC+AS  T+ VL +VVD+GCC+V+ANKKPLTST+EDY+ L+ +PRRIRHESTVGAGLPVIASLNRI+SSGDPVHRI+GSLSGTLGYVMSEVEDGKPLS+VV++AKSLG+TEPDPRDD
Sbjct:   98 IDIAALLGKSTGLAFVDCSASIDTIGVLNRVVDLGCCIVLANKKPLTSTLEDYDKLVSFPRRIRHESTVGAGLPVIASLNRILSSGDPVHRIIGSLSGTLGYVMSEVEDGKPLSEVVKSAKSLGFTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: D7M071_ARALY (Homoserine dehydrogenase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_910146 PE=4 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 2.696e-54
Identity = 105/132 (79.55%), Postives = 118/132 (89.39%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCCVV+ANKKPLTST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK+LGYTEPDPRDD
Sbjct:   99 EIAKLLGKSAGLAVVDCSASMETIEILMKAVDLGCCVVLANKKPLTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKTLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: Q84JZ4_ARATH (Homoserine dehydrogenase OS=Arabidopsis thaliana GN=At5g21060 PE=2 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.024e-53
Identity = 103/132 (78.03%), Postives = 117/132 (88.64%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:   99 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: Q2V358_ARATH (Homoserine dehydrogenase OS=Arabidopsis thaliana GN=At5g21060 PE=3 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.024e-53
Identity = 103/132 (78.03%), Postives = 117/132 (88.64%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:  101 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 232

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BLAST of FG532124 vs. TrEMBL
Match: Q8LF92_ARATH (Homoserine dehydrogenase OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.132e-52
Identity = 102/132 (77.27%), Postives = 116/132 (87.88%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLS TLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:   99 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSVTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 230

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BLAST of FG532124 vs. TrEMBL
Match: D7T1I7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_9.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00037466001 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.028e-50
Identity = 95/134 (70.90%), Postives = 115/134 (85.82%), Query Frame = 1

Query:    7 IVEIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            +++ A++L K +GL  VDC+ASS T+ VL Q VD+GCC+V+ANKKPLT+ M+DY+ L+ +PRRIR+ESTVGAGLPVIASLNRI+SSGDP+  IV  LSGTLGYVMSEVE GKP SQVV+ AKSLGYTEPDPRDD
Sbjct:   98 VIDTATRLSKSTGLVVVDCSASSETMGVLNQAVDLGCCIVLANKKPLTAAMDDYDKLVAHPRRIRYESTVGAGLPVIASLNRILSSGDPIRCIVACLSGTLGYVMSEVEGGKPFSQVVKDAKSLGYTEPDPRDD 231

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BLAST of FG532124 vs. SwissProt
Match: AK1H_ECOLI (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Escherichia coli (strain K12) GN=thrA PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 1.533e-19
Identity = 48/122 (39.34%), Postives = 72/122 (59.02%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLM----KYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCT+S          +  G  VV  NKK  TS+M+ Y  L     K  R+  +++ VGAGLPVI +L  ++++GD + +  G LSG+L Y+  ++++G   S+    A+ +GYTEPDPRDD
Sbjct:  552 VDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDD 673

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BLAST of FG532124 vs. SwissProt
Match: AKH_DAUCA (Bifunctional aspartokinase/homoserine dehydrogenase, chloroplastic (Fragment) OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.606e-19
Identity = 51/122 (41.80%), Postives = 69/122 (56.56%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA S   S     +  G  V+  NKK  +  ++ Y  L    RR      +E+TV AGLP+I +L  ++ +GD + RI G  SGTL Y+ +  +   P S+VV  AK+ GYTEPDPRDD
Sbjct:  651 VDCTADSEVASHYHDWLCRGIHVITPNKKANSGPLDQYLKLRALQRRSYTHYFYEATVVAGLPIITTLQGLLETGDKILRIEGIFSGTLSYIFNNFKSTTPFSEVVSEAKAAGYTEPDPRDD 772

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BLAST of FG532124 vs. SwissProt
Match: AKH1_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Zea mays GN=AKHSDH1 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.606e-19
Identity = 50/122 (40.98%), Postives = 68/122 (55.74%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPR----RIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA +   S     +  G  V+  NKK  +  ++ Y  L    R       +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +  E  +  S VV  AK  GYTEPDPRDD
Sbjct:  650 VDCTADTSVASHYYDWLKKGIHVITPNKKANSGPLDRYLKLRTLQRASYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGIFSGTLSYIFNNFEGARTFSDVVAEAKKAGYTEPDPRDD 771

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BLAST of FG532124 vs. SwissProt
Match: AKH_HAEIN (Bifunctional aspartokinase/homoserine dehydrogenase OS=Haemophilus influenzae GN=thrA PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.694e-18
Identity = 47/123 (38.21%), Postives = 72/123 (58.54%), Query Frame = 1

Query:   52 FVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRH----ESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            FVDCT++     +  + +  G  VV  NKK  T  +  Y  L +  +  +H    E+ VGAGLPVI +L  ++++GD +    G LSG+L ++  ++E+G  LS+V   A+  G+TEPDPRDD
Sbjct:  551 FVDCTSAESVAGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSLSFIFGKLEEGLSLSEVTALAREKGFTEPDPRDD 673

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BLAST of FG532124 vs. SwissProt
Match: AKH2_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Zea mays GN=AKHSDH2 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.694e-18
Identity = 49/122 (40.16%), Postives = 68/122 (55.74%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPR----RIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA +   S     +  G  V+  NKK  +  ++ Y  L    R       +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +  E  +  S VV  A+  GYTEPDPRDD
Sbjct:  647 VDCTADTSVASHYYDWLKKGIHVITPNKKANSGPLDQYLKLRTMQRASYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGIFSGTLSYIFNNFEGTRAFSDVVAEAREAGYTEPDPRDD 768

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BLAST of FG532124 vs. SwissProt
Match: DHOM_YEAST (Homoserine dehydrogenase OS=Saccharomyces cerevisiae GN=HOM6 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.775e-18
Identity = 51/125 (40.80%), Postives = 70/125 (56.00%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRR---IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGK----PLSQVVRAAKSLGYTEPDPRDD 408
            VD T+S+       + V+ G  +   NKK  +S +  ++ L         + HE+TVGAGLP+I+ L  II +GD V +I G  SGTL Y+ +E    +      S VV+ AK LGYTEPDPRDD
Sbjct:   90 VDNTSSAYIAGFYTKFVENGISIATPNKKAFSSDLATWKALFSNKPTNGFVYHEATVGAGLPIISFLREIIQTGDEVEKIEGIFSGTLSYIFNEFSTSQANDVKFSDVVKVAKKLGYTEPDPRDD 214

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BLAST of FG532124 vs. SwissProt
Match: AKH2_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH2 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 4.930e-18
Identity = 49/122 (40.16%), Postives = 70/122 (57.38%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA S   S     +  G  V+  NKK  +  ++ Y  L    R+      +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +     +  S+VV  AK+ G+TEPDPRDD
Sbjct:  646 VDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQYLKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVGDRSFSEVVTEAKNAGFTEPDPRDD 767

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BLAST of FG532124 vs. SwissProt
Match: AK1H_SERMA (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Serratia marcescens GN=thrA PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.439e-18
Identity = 48/122 (39.34%), Postives = 71/122 (58.20%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYP----RRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCT+S          +  G  VV  NKK  TS+M  Y+ L        R+  +++ VGAGLPVI +L  ++++GD + R  G LSG+L ++  ++++G  LS     A++ GYTEPDPRDD
Sbjct:  552 VDCTSSQAVADQYVDFLADGFHVVTPNKKANTSSMNYYQQLRAAAAGSHRKFLYDTNVGAGLPVIENLQNLLNAGDELVRFSGILSGSLSFIFGKLDEGLSLSAATLQARANGYTEPDPRDD 673

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BLAST of FG532124 vs. SwissProt
Match: AKH_BUCBP (Bifunctional aspartokinase/homoserine dehydrogenase OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) GN=thrA PE=3 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.434e-17
Identity = 44/122 (36.07%), Postives = 73/122 (59.84%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENL----MKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCT+     +     ++ G  +V  NKK  T+  + YE++     K  ++  +E+ VGAGLPVI +L  ++ +GD +    G LSG+L ++  ++ED   LS+  + A+SLG+TEP+P+DD
Sbjct:  553 VDCTSDKNIANQYISFINCGFHIVTPNKKANTTNWKYYEDIRLAAQKEKKKFFYETNVGAGLPVIENLKNLLRTGDTLIHFKGILSGSLSFIFGKLEDNISLSEATKQAQSLGFTEPNPKDD 674

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BLAST of FG532124 vs. SwissProt
Match: AKH1_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH1 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.447e-17
Identity = 48/122 (39.34%), Postives = 69/122 (56.56%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA +   S     +  G  VV  NKK  +  ++ Y  +    R+      +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +     +  S+VV  AK  G+TEPDPRDD
Sbjct:  641 VDCTADADIASCYYDWLLRGIHVVTPNKKANSGPLDQYLKIRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGIFSGTLSYLFNNFVGTRSFSEVVAEAKQAGFTEPDPRDD 762

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BLAST of FG532124 vs. TAIR peptide
Match: AT5G21060.3 (| Symbols: | Glyceraldehyde-3-phosphate dehydrogenase-like family protein | chr5:7149153-7152745 REVERSE LENGTH=378)

HSP 1 Score: 212.616 bits (540), Expect = 5.724e-56
Identity = 103/132 (78.03%), Postives = 117/132 (88.64%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:  101 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 232

[Close]

BLAST of FG532124 vs. TAIR peptide
Match: AT5G21060.2 (| Symbols: | Glyceraldehyde-3-phosphate dehydrogenase-like family protein | chr5:7149153-7152745 REVERSE LENGTH=378)

HSP 1 Score: 212.616 bits (540), Expect = 5.724e-56
Identity = 103/132 (78.03%), Postives = 117/132 (88.64%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:  101 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 232

[Close]

BLAST of FG532124 vs. TAIR peptide
Match: AT5G21060.1 (| Symbols: | Glyceraldehyde-3-phosphate dehydrogenase-like family protein | chr5:7149153-7152745 REVERSE LENGTH=376)

HSP 1 Score: 212.616 bits (540), Expect = 5.724e-56
Identity = 103/132 (78.03%), Postives = 117/132 (88.64%), Query Frame = 1

Query:   13 EIASQLGKKSGLAFVDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            EIA  LGK +GLA VDC+AS  T+ +L + VD+GCC+V+ANKKP+TST+E Y+ L  +PR IRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSE+EDGKPLSQVV+AAK LGYTEPDPRDD
Sbjct:   99 EIAKLLGKSTGLAVVDCSASMETIEILMKAVDLGCCIVLANKKPVTSTLEHYDKLALHPRFIRHESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSELEDGKPLSQVVQAAKKLGYTEPDPRDD 230

[Close]

BLAST of FG532124 vs. TAIR peptide
Match: AT4G19710.2 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729536 FORWARD LENGTH=916)

HSP 1 Score: 89.7373 bits (221), Expect = 5.596e-19
Identity = 49/122 (40.16%), Postives = 70/122 (57.38%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA S   S     +  G  V+  NKK  +  ++ Y  L    R+      +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +     +  S+VV  AK+ G+TEPDPRDD
Sbjct:  646 VDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQYLKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVGDRSFSEVVTEAKNAGFTEPDPRDD 767

[Close]

BLAST of FG532124 vs. TAIR peptide
Match: AT4G19710.1 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729278 FORWARD LENGTH=859)

HSP 1 Score: 89.7373 bits (221), Expect = 5.596e-19
Identity = 49/122 (40.16%), Postives = 70/122 (57.38%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA S   S     +  G  V+  NKK  +  ++ Y  L    R+      +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +     +  S+VV  AK+ G+TEPDPRDD
Sbjct:  646 VDCTADSAIASRYYDWLRKGIHVITPNKKANSGPLDQYLKLRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGICSGTLSYLFNNFVGDRSFSEVVTEAKNAGFTEPDPRDD 767

[Close]

BLAST of FG532124 vs. TAIR peptide
Match: AT1G31230.1 (| Symbols: AK-HSDH I, AK-HSDH | aspartate kinase-homoserine dehydrogenase i | chr1:11158744-11163055 REVERSE LENGTH=911)

HSP 1 Score: 87.4261 bits (215), Expect = 2.777e-18
Identity = 48/122 (39.34%), Postives = 69/122 (56.56%), Query Frame = 1

Query:   55 VDCTASSGTVSVLKQVVDMGCCVVMANKKPLTSTMEDYENLMKYPRRIR----HESTVGAGLPVIASLNRIISSGDPVHRIVGSLSGTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDD 408
            VDCTA +   S     +  G  VV  NKK  +  ++ Y  +    R+      +E+TVGAGLP+I++L  ++ +GD + RI G  SGTL Y+ +     +  S+VV  AK  G+TEPDPRDD
Sbjct:  641 VDCTADADIASCYYDWLLRGIHVVTPNKKANSGPLDQYLKIRDLQRKSYTHYFYEATVGAGLPIISTLRGLLETGDKILRIEGIFSGTLSYLFNNFVGTRSFSEVVAEAKQAGFTEPDPRDD 762

The following BLAST results are available for this feature:

BLAST of FG532124 vs. TrEMBL
BLAST of FG532124 vs. SwissProt
BLAST of FG532124 vs. TAIR peptide

BLAST of FG532124 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FM35_MEDTR	1.862e-63	88.97	Homoserine dehydrogenase OS=Medicago truncatula PE...	[more]
Q3S3F6_SOYBN	1.743e-61	86.03	Homoserine dehydrogenase OS=Glycine max PE=2 SV=1	[more]
C6TCU7_SOYBN	1.130e-60	85.29	Homoserine dehydrogenase OS=Glycine max PE=2 SV=1	[more]
B9MYR6_POPTR	3.077e-58	79.26	Homoserine dehydrogenase OS=Populus trichocarpa GN...	[more]
B9S6K6_RICCO	1.994e-57	78.95	Homoserine dehydrogenase OS=Ricinus communis GN=RC...	[more]
D7M071_ARALY	2.696e-54	79.55	Homoserine dehydrogenase family protein OS=Arabido...	[more]
Q84JZ4_ARATH	1.024e-53	78.03	Homoserine dehydrogenase OS=Arabidopsis thaliana G...	[more]
Q2V358_ARATH	1.024e-53	78.03	Homoserine dehydrogenase OS=Arabidopsis thaliana G...	[more]
Q8LF92_ARATH	1.132e-52	77.27	Homoserine dehydrogenase OS=Arabidopsis thaliana P...	[more]
D7T1I7_VITVI	4.028e-50	70.90	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of FG532124 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AK1H_ECOLI	1.533e-19	39.34	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_DAUCA	7.606e-19	41.80	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH1_MAIZE	7.606e-19	40.98	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_HAEIN	1.694e-18	38.21	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH2_MAIZE	1.694e-18	40.16	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
DHOM_YEAST	3.775e-18	40.80	Homoserine dehydrogenase OS=Saccharomyces cerevisi...	[more]
AKH2_ARATH	4.930e-18	40.16	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AK1H_SERMA	6.439e-18	39.34	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_BUCBP	1.434e-17	36.07	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH1_ARATH	2.447e-17	39.34	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]

BLAST of FG532124 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G21060.3	5.724e-56	78.03	\| Symbols: \| Glyceraldehyde-3-phosphate dehydroge...	[more]
AT5G21060.2	5.724e-56	78.03	\| Symbols: \| Glyceraldehyde-3-phosphate dehydroge...	[more]
AT5G21060.1	5.724e-56	78.03	\| Symbols: \| Glyceraldehyde-3-phosphate dehydroge...	[more]
AT4G19710.2	5.596e-19	40.16	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]
AT4G19710.1	5.596e-19	40.16	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]
AT1G31230.1	2.777e-18	39.34	\| Symbols: AK-HSDH I, AK-HSDH \| aspartate kinase-h...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001342	Homoserine dehydrogenase, catalytic	PFAM	PF00742	Homoserine_dh	coord: 77..136 score: 5.8
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 14..98 score: 9
None	No IPR available	GENE3D	3.30.360.10	no description	coord: 99..136 score: 1.4
None	No IPR available	PANTHER	PTHR21499	ASPARTATE KINASE	coord: 2..136 score: 7.9
None	No IPR available	PANTHER	PTHR21499:SF1	ASPARTATE KINASE	coord: 2..136 score: 7.9
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 17..96 score: 2.2
None	No IPR available	SUPERFAMILY	55347	Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain	coord: 77..136 score: 2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG532124 ID=FG532124; Name=FG532124; organism=Pisum sativum; type=EST; length=409bp
GGACAGATTGTAGAGATTGCATCTCAACTTGGTAAAAAATCAGGTTTGGC
CTTTGTAGATTGCACTGCTAGCTCCGGCACTGTTTCAGTGCTAAAACAAG
TGGTTGATATGGGTTGTTGTGTTGTTATGGCAAATAAAAAGCCTCTTACT
TCTACAATGGAGGATTATGAAAACCTTATGAAATATCCACGTCGAATCCG
ACATGAGTCAACTGTTGGTGCTGGTCTTCCTGTGATAGCATCCCTGAATC
GCATTATCTCATCTGGCGATCCAGTTCATCGGATTGTTGGAAGTTTGAGT
GGAACATTGGGATATGTAATGAGTGAGGTTGAAGATGGAAAACCGCTAAG
CCAAGTTGTTAGAGCTGCTAAAAGTCTGGGGTACACTGAACCAGATCCAC
GTGATGACC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006520	amino acid metabolic process
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001342	HDH_cat
IPR016040	NAD(P)-bd_dom

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