FG533750

EST Overview


Name	FG533750
Unique Name	FG533750
Type	EST
Organism	Pisum sativum (pea)
Sequence length	356

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG533750 vs. TrEMBL
Match: D7UB90_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_24.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00019326001 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 4.220e-47
Identity = 94/117 (80.34%), Postives = 109/117 (93.16%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLPFHMV ATY+VK A+KQLYSENGR P++ EVAEATGLSMKRLNAV +TPKAPRSL+QKIGINQ+LKPSEV+ADP+AET EE LIKQFM++DLEKVL+SLNPREKQVIR R+G++
Sbjct:  412 IRLPFHMVEATYRVKEARKQLYSENGRHPNNEEVAEATGLSMKRLNAVLLTPKAPRSLDQKIGINQNLKPSEVIADPDAETAEELLIKQFMREDLEKVLNSLNPREKQVIRWRYGLE 528

[Close]

BLAST of FG533750 vs. TrEMBL
Match: B9SDA3_RICCO (RNA polymerase sigma factor rpoD1, putative OS=Ricinus communis GN=RCOM_1515490 PE=4 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.358e-45
Identity = 91/117 (77.78%), Postives = 105/117 (89.74%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLPFHMV ATY+VK A+KQLYSENGR PDD EVAEA GLSMKRL+AV +TPKAPRSL+QKIG+N  LKPSEV+ADPEAET EE L+KQFMK+DLEKVL++LNPRE QV+R RFG++
Sbjct:  406 IRLPFHMVEATYRVKEARKQLYSENGRHPDDEEVAEAAGLSMKRLSAVLLTPKAPRSLDQKIGMNMDLKPSEVIADPEAETAEELLMKQFMKQDLEKVLNTLNPRETQVVRWRFGLE 522

[Close]

BLAST of FG533750 vs. TrEMBL
Match: B9IPZ1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_780378 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.730e-44
Identity = 89/117 (76.07%), Postives = 102/117 (87.18%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLPFHMV ATY+VK A+KQ YSENGR PDD EVAEATGLSMKRL+AV +TPKAPRSL+QK+G N  LK SEV ADPEAET E+ L+K+FMKKDLE+VL+SL+PRE QVIR RFGM+
Sbjct:  401 IRLPFHMVDATYRVKEARKQFYSENGRHPDDKEVAEATGLSMKRLSAVLLTPKAPRSLDQKMGFNMDLKLSEVTADPEAETAEDLLMKEFMKKDLERVLESLSPRENQVIRWRFGME 517

[Close]

BLAST of FG533750 vs. TrEMBL
Match: Q710C3_SPIOL (Sigma factor OS=Spinacia oleracea GN=sig2 PE=2 SV=2)

HSP 1 Score: 178.718 bits (452), Expect = 1.660e-43
Identity = 89/117 (76.07%), Postives = 104/117 (88.89%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLPFHMV ATY+VK A+KQL SENG+QP++ EVAEATGLSMKRL AV MTPKAPRSL+QKIG N +LKPSEV+ADPEAET E+ L KQFM++DLEKVLD+L+ REKQVIR RFG++
Sbjct:  412 IRLPFHMVEATYRVKEARKQLLSENGKQPNNEEVAEATGLSMKRLTAVLMTPKAPRSLDQKIGFNMNLKPSEVIADPEAETAEDLLTKQFMRQDLEKVLDTLSSREKQVIRWRFGLE 528

[Close]

BLAST of FG533750 vs. TrEMBL
Match: B9I6F1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834586 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 8.237e-43
Identity = 88/117 (75.21%), Postives = 100/117 (85.47%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLPFHMV ATY+VK A+KQLYSENGR PDD EVAEA GLSMKRL+AV +TPKAPRSL+QK+G N  LK SEV ADPEAET E+ L+K+FMKKDL KVL SL+PRE QV+R RFGM+
Sbjct:  404 IRLPFHMVDATYRVKEARKQLYSENGRHPDDKEVAEAAGLSMKRLSAVLLTPKAPRSLDQKMGFNMDLKLSEVTADPEAETAEDLLMKEFMKKDLAKVLGSLSPRENQVVRWRFGME 520

[Close]

BLAST of FG533750 vs. TrEMBL
Match: Q9MTH3_SINAL (Sigma factor 2 OS=Sinapis alba GN=sig2 PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.076e-42
Identity = 88/118 (74.58%), Postives = 105/118 (88.98%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+QP + EVAEATGLSMKRL AV ++PK PRSL+QKIGIN +LKPSEV+ADPEAET E+ L+KQFM++DL+KVLDSL+ REKQVIR RFGM+
Sbjct:  406 MIRLPFHMVEATYRVKEARKQLYSETGKQPKNEEVAEATGLSMKRLMAVLLSPKPPRSLDQKIGINLNLKPSEVIADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGME 523

[Close]

BLAST of FG533750 vs. TrEMBL
Match: Q8GWC1_ARATH (Putative plastid RNA polymerase sigma-subunit SIG1 OS=Arabidopsis thaliana GN=At1g08540/F22O13_2 PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.835e-42
Identity = 86/118 (72.88%), Postives = 104/118 (88.14%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+ P + E+AEATGLSMKRL AV ++PK PRSL+QKIG+NQ+LKPSEV+ADPEA TPE+ LIK+FM++DL+KVLDSL  REKQVIR RFGM+
Sbjct:  406 MIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIADPEAVTPEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGME 523

[Close]

BLAST of FG533750 vs. TrEMBL
Match: O22056_ARATH (At1g08540 OS=Arabidopsis thaliana GN=SIG1 PE=2 SV=2)

HSP 1 Score: 172.17 bits (435), Expect = 1.553e-41
Identity = 85/118 (72.03%), Postives = 103/118 (87.29%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+ P + E+AEATGLSMKRL AV ++PK PRSL+QKIG+NQ+LKPSEV+ADPEA T E+ LIK+FM++DL+KVLDSL  REKQVIR RFGM+
Sbjct:  406 MIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGME 523

[Close]

BLAST of FG533750 vs. TrEMBL
Match: D7KIT0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_470915 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.553e-41
Identity = 85/118 (72.03%), Postives = 103/118 (87.29%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+ P + E+AEATGLSMKRL AV ++PK PRSL+QKIG+NQ+LKPSEV+ADPEA T E+ LIK+FM++DL+KVLDSL  REKQVIR RFGM+
Sbjct:  406 MIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGME 523

[Close]

BLAST of FG533750 vs. TrEMBL
Match: O22455_ARATH (Sigma factor 2 OS=Arabidopsis thaliana GN=SIG2 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.520e-41
Identity = 84/118 (71.19%), Postives = 103/118 (87.29%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+ P + E+AEATGLSMKRL AV ++PK PRSL++KIG+NQ+LKPSEV+ADPEA T E+ LIK+FM++DL+KVLDSL  REKQVIR RFGM+
Sbjct:  408 MIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDRKIGMNQNLKPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGME 525

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_ANASP (RNA polymerase sigma factor rpoD OS=Anabaena sp. (strain PCC 7120) GN=rpoD PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.301e-18
Identity = 45/117 (38.46%), Postives = 75/117 (64.10%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLP H+     ++K   K L  E GR+P + E+A    +++++L  +  + + P SLE  IG  +  +  + + + + ETPE+Q+ K  +++DLEKVLDSL+PRE+ V+RLR+G+D
Sbjct:  229 IRLPVHLYETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFI-ESDGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLD 344

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_SYNY3 (RNA polymerase sigma factor rpoD OS=Synechocystis sp. (strain PCC 6803) GN=rpoD PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 4.185e-17
Identity = 42/117 (35.90%), Postives = 74/117 (63.25%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLP H+     ++K   K L  E  R+P + E+AE   +++++L  +  + + P SLE  IG  +  +  + + + + ETPE+++ K  +++DLE VLD+L+PRE+ V+RLR+G+D
Sbjct:  264 IRLPVHLYETISRIKKTTKLLSQEMRRKPTEEEIAEKMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFI-EADGETPEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLD 379

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD1_SYNE7 (RNA polymerase sigma factor rpoD1 OS=Synechococcus elongatus (strain PCC 7942) GN=rpoD1 PE=3 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 1.590e-16
Identity = 41/117 (35.04%), Postives = 73/117 (62.39%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLP H+     ++K   K L  E GR+P + E+A    +++++L  +  + + P SLE  IG  +  +  + + + + ETPE+++ K  +++DLE VL +L+PRE+ V+RLR+G+D
Sbjct:  238 IRLPVHLYETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFI-EADGETPEDEVAKNLLREDLEGVLSTLSPRERDVLRLRYGLD 353

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_MICAE (RNA polymerase sigma factor rpoD1 OS=Microcystis aeruginosa GN=rpoD1 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.713e-16
Identity = 41/117 (35.04%), Postives = 73/117 (62.39%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLP H+     ++K   K L  E  R+P + E+A    +++++L  +  + + P SLE  IG  +  +  + + + + ETPE+++ K  +++DLE VLD+L+PRE+ V+RLR+G+D
Sbjct:  255 IRLPVHLYETISRIKKTTKILSQEMRRKPTEEEIATKMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFI-EADGETPEDEVSKNLLREDLENVLDTLSPRERDVLRLRYGLD 370

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_BACSU (RNA polymerase sigma factor rpoD OS=Bacillus subtilis GN=sigA PE=1 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 4.627e-16
Identity = 43/117 (36.75%), Postives = 66/117 (56.41%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IR+P HMV    K+   Q+QL  + GR+P   E+AE   L+ +++  +    + P SLE  IG        + + D EA +P +    + +K+ LE VLD+L  RE+ V+RLRFG+D
Sbjct:  209 IRIPVHMVETINKLIRVQRQLLQDLGREPTPEEIAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLD 325

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPSD2_BACSP (RNA polymerase sigma factor rpoD OS=Bacillus sp. GN=sigA PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 7.893e-16
Identity = 44/117 (37.61%), Postives = 65/117 (55.56%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IR+P HMV    K+    +QL  E GR+P   E+A    LS++++  +    + P SLE  IG        + + D EA TP +    + +K+ LE VLD+L  RE+ V+RLRFG+D
Sbjct:  215 IRIPVHMVETINKLIRVSRQLLQELGREPTPEEIAAEMELSVEKVREIMKIAQEPVSLETPIGEEDDSHLGDFIEDQEALTPADAAAYELLKEQLEDVLDTLTEREENVLRLRFGLD 331

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_HELPY (RNA polymerase sigma factor rpoD OS=Helicobacter pylori GN=rpoD PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 7.893e-16
Identity = 41/116 (35.34%), Postives = 66/116 (56.90%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGM 351
            IR+P HM+    ++    ++   ENG++PD   VAE  GLS+ ++  V    K P SLE  +G +   K  + + D    +  + ++++ +K  +E VLD LN REK VIR+RFG+
Sbjct:  507 IRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDKVKNVIKVTKEPISLETPVGNDDDGKFGDFVEDKNIVSSIDHIMREDLKAQIESVLDQLNEREKAVIRMRFGL 622

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_STAAN (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain N315) GN=rpoD PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.031e-15
Identity = 43/117 (36.75%), Postives = 64/117 (54.70%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IR+P HMV    K+   Q+QL  + GR P   E+ E   L  +++  V    + P SLE  IG        + + D EA++P +    + +K+ LE VLD+L  RE+ V+RLRFG+D
Sbjct:  206 IRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLD 322

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_STAAW (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain MW2) GN=rpoD PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.346e-15
Identity = 42/117 (35.90%), Postives = 64/117 (54.70%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IR+P HMV    K+   Q+QL  + GR P   E+ E   L  +++  +    + P SLE  IG        + + D EA++P +    + +K+ LE VLD+L  RE+ V+RLRFG+D
Sbjct:  206 IRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLD 322

[Close]

BLAST of FG533750 vs. SwissProt
Match: RPOD_STAAS (RNA polymerase sigma factor rpoD OS=Staphylococcus aureus (strain MSSA476) GN=rpoD PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.346e-15
Identity = 42/117 (35.90%), Postives = 64/117 (54.70%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IR+P HMV    K+   Q+QL  + GR P   E+ E   L  +++  +    + P SLE  IG        + + D EA++P +    + +K+ LE VLD+L  RE+ V+RLRFG+D
Sbjct:  206 IRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLD 322

[Close]

BLAST of FG533750 vs. TAIR peptide
Match: AT1G08540.1 (| Symbols: SIGB, SIG1, SIG2, SIGA, ATSIG1, ABC1, ATSIG2 | RNApolymerase sigma subunit 2 | chr1:2703461-2706696 FORWARD LENGTH=572)

HSP 1 Score: 172.17 bits (435), Expect = 5.832e-44
Identity = 85/118 (72.03%), Postives = 103/118 (87.29%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +IRLPFHMV ATY+VK A+KQLYSE G+ P + E+AEATGLSMKRL AV ++PK PRSL+QKIG+NQ+LKPSEV+ADPEA T E+ LIK+FM++DL+KVLDSL  REKQVIR RFGM+
Sbjct:  406 MIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIADPEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGME 523

[Close]

BLAST of FG533750 vs. TAIR peptide
Match: AT2G36990.1 (| Symbols: SIGF, SIG6, ATSIG6, SOLDAT8 | RNApolymerase sigma-subunit F | chr2:15537502-15540016 REVERSE LENGTH=547)

HSP 1 Score: 71.2478 bits (173), Expect = 1.401e-13
Identity = 38/117 (32.48%), Postives = 65/117 (55.56%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            IRLP ++     KV  A+K    E   +P   E+A   G+S ++L+ +    + P S++Q I  +Q     E+  D   ETP   + KQ M+  +  +L+ L+P+E+++I+LRFG+D
Sbjct:  382 IRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGID 498

[Close]

BLAST of FG533750 vs. TAIR peptide
Match: AT3G53920.1 (| Symbols: SIGC, SIG3 | RNApolymerase sigma-subunit C | chr3:19961041-19963820 REVERSE LENGTH=571)

HSP 1 Score: 70.0922 bits (170), Expect = 3.121e-13
Identity = 37/117 (31.62%), Postives = 69/117 (58.97%), Query Frame = 1

Query:    4 IRLPFHMVGATYKVKAAQKQLYSENG-RQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGM 351
            + +P  ++     ++ A+K L + +G +   D E+A+ TG S+K++ A     K   S+++K+G   + K  E   D   E+PEE +++Q  ++D+  +L+ L PREKQV+ LR+G+
Sbjct:  407 VHIPSSIIRTINHIQKARKTLKTSHGIKYAADEEIAKLTGHSVKKIRAANQCLKVVGSIDKKVGDCFTTKFLEFTPDTTMESPEEAVMRQSARRDIHDLLEGLEPREKQVMVLRYGL 523

[Close]

BLAST of FG533750 vs. TAIR peptide
Match: AT5G13730.1 (| Symbols: SIG4, SIGD | sigma factor 4 | chr5:4429132-4430744 REVERSE LENGTH=419)

HSP 1 Score: 64.3142 bits (155), Expect = 1.713e-11
Identity = 36/118 (30.51%), Postives = 63/118 (53.39%), Query Frame = 1

Query:    1 IIRLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            +++LP  M   T KV  A   L  +  RQP   E+AE   L++  +       ++P SL++    N  +   E++  P+   PEE + ++ MK ++E++L SL  RE +V+ L FG++
Sbjct:  253 LVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEEIAEHLNLNVSAVRLAVERSRSPVSLDRVASQNGRMTLQEIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGLN 370

[Close]

BLAST of FG533750 vs. TAIR peptide
Match: AT5G24120.1 (| Symbols: SIGE, SIG5, ATSIG5 | sigma factor E | chr5:8157794-8159746 REVERSE LENGTH=517)

HSP 1 Score: 53.5286 bits (127), Expect = 3.023e-8
Identity = 34/116 (29.31%), Postives = 55/116 (47.41%), Query Frame = 1

Query:    7 RLPFHMVGATYKVKAAQKQLYSENGRQPDDAEVAEATGLSMKRLNAVYMTPKAPRSLEQKIGINQSLKPSEVLADPEAETPEEQLIKQFMKKDLEKVLDSLNPREKQVIRLRFGMD 354
            R+PF +     ++  A+ +L+ E GR P + EV E   ++ +R   V    K   SL  K  + Q  +    + D +            ++  L+ VLDSL P+E  VIR R+G+D
Sbjct:  357 RVPFGLESVRVEIYKAKTELWFEMGRPPTEDEVVERLKITPERYREVLRAAKPVLSLNSKHSVTQE-EFINGITDVDGVGANNSRQPALLRLALDDVLDSLKPKESLVIRQRYGLD 471

The following BLAST results are available for this feature:

BLAST of FG533750 vs. TrEMBL
BLAST of FG533750 vs. SwissProt
BLAST of FG533750 vs. TAIR peptide

BLAST of FG533750 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7UB90_VITVI	4.220e-47	80.34	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SDA3_RICCO	1.358e-45	77.78	RNA polymerase sigma factor rpoD1, putative OS=Ric...	[more]
B9IPZ1_POPTR	9.730e-44	76.07	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q710C3_SPIOL	1.660e-43	76.07	Sigma factor OS=Spinacia oleracea GN=sig2 PE=2 SV=...	[more]
B9I6F1_POPTR	8.237e-43	75.21	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9MTH3_SINAL	1.076e-42	74.58	Sigma factor 2 OS=Sinapis alba GN=sig2 PE=2 SV=1	[more]
Q8GWC1_ARATH	1.835e-42	72.88	Putative plastid RNA polymerase sigma-subunit SIG1...	[more]
O22056_ARATH	1.553e-41	72.03	At1g08540 OS=Arabidopsis thaliana GN=SIG1 PE=2 SV=...	[more]
D7KIT0_ARALY	1.553e-41	72.03	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
O22455_ARATH	4.520e-41	71.19	Sigma factor 2 OS=Arabidopsis thaliana GN=SIG2 PE=...	[more]

BLAST of FG533750 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPOD_ANASP	1.301e-18	38.46	RNA polymerase sigma factor rpoD OS=Anabaena sp. (...	[more]
RPOD_SYNY3	4.185e-17	35.90	RNA polymerase sigma factor rpoD OS=Synechocystis ...	[more]
RPOD1_SYNE7	1.590e-16	35.04	RNA polymerase sigma factor rpoD1 OS=Synechococcus...	[more]
RPOD_MICAE	2.713e-16	35.04	RNA polymerase sigma factor rpoD1 OS=Microcystis a...	[more]
RPOD_BACSU	4.627e-16	36.75	RNA polymerase sigma factor rpoD OS=Bacillus subti...	[more]
RPSD2_BACSP	7.893e-16	37.61	RNA polymerase sigma factor rpoD OS=Bacillus sp. G...	[more]
RPOD_HELPY	7.893e-16	35.34	RNA polymerase sigma factor rpoD OS=Helicobacter p...	[more]
RPOD_STAAN	1.031e-15	36.75	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAW	1.346e-15	35.90	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]
RPOD_STAAS	1.346e-15	35.90	RNA polymerase sigma factor rpoD OS=Staphylococcus...	[more]

BLAST of FG533750 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G08540.1	5.832e-44	72.03	\| Symbols: SIGB, SIG1, SIG2, SIGA, ATSIG1, ABC1, A...	[more]
AT2G36990.1	1.401e-13	32.48	\| Symbols: SIGF, SIG6, ATSIG6, SOLDAT8 \| RNApolyme...	[more]
AT3G53920.1	3.121e-13	31.62	\| Symbols: SIGC, SIG3 \| RNApolymerase sigma-subuni...	[more]
AT5G13730.1	1.713e-11	30.51	\| Symbols: SIG4, SIGD \| sigma factor 4 \| chr5:4429...	[more]
AT5G24120.1	3.023e-8	29.31	\| Symbols: SIGE, SIG5, ATSIG5 \| sigma factor E \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007624	RNA polymerase sigma-70 region 3	PFAM	PF04539	Sigma70_r3	coord: 13..86 score: 4.1
IPR011991	Winged helix-turn-helix transcription repressor DNA-binding	GENE3D	1.10.10.10	no description	coord: 4..49 score: 1.8e-10coord: 94..118 score: 0.0
IPR013324	RNA polymerase sigma factor, region 3/4	SUPERFAMILY	88659	Sigma3 and sigma4 domains of RNA polymerase sigma factors	coord: 2..60 score: 6.1e-13coord: 61..118 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 12..27 score: NAcoord: 33..47 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG533750 ID=FG533750; Name=FG533750; organism=Pisum sativum; type=EST; length=356bp
ATTATTCGCTTGCCATTCCACATGGTGGGGGCAACTTACAAAGTGAAGGC
GGCACAAAAACAGTTGTACAGTGAAAATGGAAGACAACCAGACGATGCAG
AAGTTGCTGAAGCAACTGGGCTGTCAATGAAGAGGCTTAATGCTGTTTAT
ATGACCCCAAAAGCTCCCAGATCTCTAGAACAGAAAATTGGGATCAACCA
GAGTCTTAAACCTTCAGAAGTACTTGCTGATCCGGAAGCTGAAACACCCG
AAGAACAACTCATTAAGCAGTTCATGAAAAAGGACCTAGAAAAGGTACTA
GACAGCCTGAATCCAAGAGAGAAACAGGTGATTAGATTGAGATTTGGTAT
GGACGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	DNA-binding transcription factor activity
GO:0016987	sigma factor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006352	DNA-templated transcription initiation

Vocabulary: **INTERPRO**
Term	Definition
IPR007624	RNA_pol_sigma70_r3
IPR011991	ArsR-like_HTH
IPR013324	RNA_pol_sigma_r3/r4-like

Search form