FG534751

EST Overview


Name	FG534751
Unique Name	FG534751
Type	EST
Organism	Pisum sativum (pea)
Sequence length	673

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG534751 vs. TrEMBL
Match: D7KUD3_ARALY (ATP dependent DNA ligase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_338905 PE=4 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 1.090e-84
Identity = 141/169 (83.43%), Postives = 160/169 (94.67%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTE 509
            D WLKVKRDYV+GL DTLDLVPIGAWHGNGRKAGWYSP LMACFNPETEE+QSVCRVMSGFSD+FYIEMKE++SGDK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH AA+GLVHPSRGIS+RFPRFI  V+DRNPEECSTAADI EMFH+QTRK+++T++
Sbjct: 1244 DSWLKVKRDYVDGLGDTLDLVPIGAWHGNGRKAGWYSPLLMACFNPETEEFQSVCRVMSGFSDAFYIEMKELYSGDKILTKKPPYYRTGETPDMWFSTEVVWEIRGADFTVSPVHSAALGLVHPSRGISVRFPRFIRKVTDRNPEECSTAADIAEMFHAQTRKLNITSQ 1412

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BLAST of FG534751 vs. TrEMBL
Match: A5AGP3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041957 PE=4 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 1.205e-83
Identity = 142/167 (85.03%), Postives = 154/167 (92.22%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVT 503
            D WLKVKRDYVEGLND+LDLVPIGAWHGNGRKAGWYSPFLMAC+NP+TEE+QSVCRVMSGFSD+FY EMKE F+ DK+LSKKPPYYQT E PDMWF P+L+WEIRGADFTVSPVH AAIGLVHPSRGIS+RFPRFI  + DR PEECSTAADI +MFHSQTRKMDVT
Sbjct:  103 DTWLKVKRDYVEGLNDSLDLVPIGAWHGNGRKAGWYSPFLMACYNPDTEEFQSVCRVMSGFSDNFYKEMKEFFNEDKILSKKPPYYQTAELPDMWFTPELIWEIRGADFTVSPVHQAAIGLVHPSRGISVRFPRFIRPIMDRRPEECSTAADIADMFHSQTRKMDVT 269

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BLAST of FG534751 vs. TrEMBL
Match: B9H344_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_555902 PE=4 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 4.578e-83
Identity = 144/175 (82.29%), Postives = 159/175 (90.86%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIE------MKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTE 509
            D WLKVK+DYVEGLND+LDLVPIGAWHGNGRKAGWYSPFLMAC+NPETEE+QSVCRVMSGFSD+FYIE      MKE FSGD++L+KKPPYY+T E PDMWF P++VWEIRGADFT+SPVH AA+GLVH SRGISIRFPRFI S+SDRNPEECSTAADI EMF+SQTRKMDVT E
Sbjct:   52 DAWLKVKKDYVEGLNDSLDLVPIGAWHGNGRKAGWYSPFLMACYNPETEEFQSVCRVMSGFSDAFYIEVITELQMKEFFSGDRILAKKPPYYRTVEAPDMWFSPEVVWEIRGADFTISPVHQAAVGLVHQSRGISIRFPRFIHSISDRNPEECSTAADIAEMFNSQTRKMDVTAE 226

[Close]

BLAST of FG534751 vs. TrEMBL
Match: D7TSX4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_6.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00030942001 PE=4 SV=1)

HSP 1 Score: 310.842 bits (795), Expect = 5.979e-83
Identity = 141/167 (84.43%), Postives = 153/167 (91.62%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVT 503
            D WLKVKRDYVEGLND+LDLVPIGAWHGNGRKAGWYSPFLMAC+NP+TEE+QSVCRVMSGFSD+FY EMKE F+ DK+LSKKPPYYQT E PDMWF P+L+WEIRGADFTVSPVH AAIGLVHPSRGIS+RFPRFI  + DR PEECSTAADI +MFH QTRKMDVT
Sbjct: 1221 DTWLKVKRDYVEGLNDSLDLVPIGAWHGNGRKAGWYSPFLMACYNPDTEEFQSVCRVMSGFSDNFYKEMKEFFNEDKILSKKPPYYQTAELPDMWFTPELIWEIRGADFTVSPVHQAAIGLVHPSRGISVRFPRFIRPIMDRRPEECSTAADIADMFHFQTRKMDVT 1387

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BLAST of FG534751 vs. TrEMBL
Match: Q9C9M5_ARATH (DNA ligase OS=Arabidopsis thaliana GN=F4N21_14 PE=3 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 4.737e-80
Identity = 139/186 (74.73%), Postives = 158/186 (84.95%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIE-----------------MKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTE 509
            D WLKVKRDYV+GL DTLDLVPIGAW+GNGRKAGWYSPFLMACFNPETEE+QSVCRVMSGFSD+FYIE                 MKE +S DK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH A++GLVHPSRGIS+RFPRFI  V+DRNPEECSTA DI EMFH+QTRKM++T++
Sbjct: 1231 DSWLKVKRDYVDGLGDTLDLVPIGAWYGNGRKAGWYSPFLMACFNPETEEFQSVCRVMSGFSDAFYIEVMKSSTLTLLFNDETDGMKEFYSEDKILAKKPPYYRTGETPDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPRFISKVTDRNPEECSTATDIAEMFHAQTRKMNITSQ 1416

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BLAST of FG534751 vs. TrEMBL
Match: B9SPY8_RICCO (DNA ligase OS=Ricinus communis GN=RCOM_0466830 PE=3 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 2.599e-78
Identity = 134/166 (80.72%), Postives = 151/166 (90.96%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDV 500
            D WLKVKRDYVE LN++LDLVPIGAWHGNGRKAGWYSPFLMAC+NPETEE+QSVCRVMSGFSD+FY EMKE FSGD++L KKP YY+T E PD WF P++VWEIRGA+FTVSPVH AA+GLVHPSRGIS+RFPRFI SV+DRN EECSTA DI +MF+SQTRKMD+
Sbjct: 1191 DTWLKVKRDYVEELNNSLDLVPIGAWHGNGRKAGWYSPFLMACYNPETEEFQSVCRVMSGFSDAFYTEMKEYFSGDRILLKKPHYYRTAEVPDKWFSPEVVWEIRGAEFTVSPVHQAAVGLVHPSRGISMRFPRFIRSVTDRNAEECSTAEDIADMFNSQTRKMDI 1356

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BLAST of FG534751 vs. TrEMBL
Match: Q0JKB6_ORYSJ (Os01g0685500 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os01g0685500 PE=4 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 6.202e-72
Identity = 124/170 (72.94%), Postives = 147/170 (86.47%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTEG 512
            D WLKVKRDYV GL D+LDLVPIGAW+GNGRKAGWYSPFLMAC+NPE EE+QSVCRVMSGFSD FY EMKE +SGD++L KKP YY+T E P++WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +QTRKM+V+++G
Sbjct:  145 DSWLKVKRDYV-GLGDSLDLVPIGAWYGNGRKAGWYSPFLMACYNPEYEEFQSVCRVMSGFSDEFYKEMKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 313

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BLAST of FG534751 vs. TrEMBL
Match: B9EYN2_ORYSJ (DNA ligase OS=Oryza sativa subsp. japonica GN=OsJ_03041 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 4.465e-54
Identity = 116/235 (49.36%), Postives = 139/235 (59.15%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIE--------------MKEIFSGDKVL-------------------------SKKPPYYQTGETPDM--------------------------WFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTEG 512
            D WLKVKRDYV GL D+LDLVPIGAW+GNGRKAGWYSPFLMAC+NPE EE+QSVCRVMSGFSD FY E               + +  G  +L                            PP  + GE+ +M                          WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +QTRKM+V+++G
Sbjct: 1217 DSWLKVKRDYV-GLGDSLDLVPIGAWYGNGRKAGWYSPFLMACYNPEYEEFQSVCRVMSGFSDEFYKEPQPAAALADCRRCAQRPVAGGLPLLDCSACRCATSRSPMLSPVVTHQLDAGSTPPIARDGESMEMMKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 1450

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BLAST of FG534751 vs. TrEMBL
Match: B8A7M7_ORYSI (DNA ligase OS=Oryza sativa subsp. indica GN=OsI_03304 PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 4.465e-54
Identity = 116/235 (49.36%), Postives = 139/235 (59.15%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIE--------------MKEIFSGDKVL-------------------------SKKPPYYQTGETPDM--------------------------WFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTEG 512
            D WLKVKRDYV GL D+LDLVPIGAW+GNGRKAGWYSPFLMAC+NPE EE+QSVCRVMSGFSD FY E               + +  G  +L                            PP  + GE+ +M                          WF  + VWEIRGAD T+SPVHHAAIGLVHPSRGIS+R PR+I S  DR+PE+CSTA D+  +F +QTRKM+V+++G
Sbjct:  988 DSWLKVKRDYV-GLGDSLDLVPIGAWYGNGRKAGWYSPFLMACYNPEYEEFQSVCRVMSGFSDEFYKEPQPAATLADCRRCAQRPVAGGLPLLDCSACRCATSRSPMLSPVVTHQLDAGSTPPIARDGESMEMMKEFYSGDRILPKKPVYYKTDELPELWFSAEQVWEIRGADLTLSPVHHAAIGLVHPSRGISVRMPRYIRSRPDRSPEDCSTATDVASLFKAQTRKMEVSSDG 1221

[Close]

BLAST of FG534751 vs. TrEMBL
Match: C1E332_9CHLO (DNA ligase OS=Micromonas sp. RCC299 GN=MICPUN_97217 PE=3 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 7.377e-49
Identity = 95/168 (56.55%), Postives = 115/168 (68.45%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSK---KPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMD 497
            D WLK+K+DY E L D+LDLVPIGAW G GRKAGWYSPFL+A ++PETE YQS+CR MSGF+D FY   +E F     L +   KPP Y T E PD+WF P  VWEIRGAD T+SP H AA G  H  RGIS+RFPRFI    D+N E+ S   ++  +F +Q  + D
Sbjct:  440 DCWLKLKKDYCENLRDSLDLVPIGAWFGQGRKAGWYSPFLLAVYDPETETYQSMCRCMSGFTDEFYRTRRENFGKPPSLVESGVKPPVYDTLEQPDVWFNPTEVWEIRGADLTLSPKHRAAAGARHEERGISLRFPRFIRVRDDKNVEDASGPEEVARLFDAQQSRYD 607

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BLAST of FG534751 vs. SwissProt
Match: DNLI3_SCHPO (DNA ligase 3 OS=Schizosaccharomyces pombe GN=adl1 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 7.770e-43
Identity = 84/162 (51.85%), Postives = 116/162 (71.60%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYY---QTGE-TPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            W+KVK+DY +   ++LDLVPIGAW+GNGRKAGW+SP L+A +NP+T  Y++VC+ MSGFSD FY E+ + +S +   S   P Y   +TG+ TP ++F PQ VWEI+GA  T SP + AA+GL+   RG+SIRFPRFI   SD+ PE+ ST + + +M+  Q
Sbjct:  611 WIKVKQDYDDEF-ESLDLVPIGAWYGNGRKAGWFSPILLAVYNPDTGAYEAVCKCMSGFSDQFYKELTQKYSLESGNSSLKPIYNFCETGKVTPQIYFAPQEVWEIKGAQITSSPAYKAALGLIQDDRGLSIRFPRFIRVRSDKGPEDASTNSILADMYMKQ 771

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BLAST of FG534751 vs. SwissProt
Match: DNLI1_ARATH (DNA ligase 1 OS=Arabidopsis thaliana GN=At1g08130 PE=2 SV=2)

HSP 1 Score: 160.999 bits (406), Expect = 5.212e-39
Identity = 69/162 (42.59%), Postives = 112/162 (69.14%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            + WLK+K+DY++ + D++DLVPI A+HG G++ G Y  FL+AC++ + EE+QS+C++ +GFSD+   E        +V++    YY+ G++  PD+WF P  VWE++ AD T+SPVH AA G+V P +GIS+RFPR +    D+ PEE +++  I +++ +Q
Sbjct:  616 NNWLKLKKDYMDSIGDSVDLVPIAAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLR-SQVIATPKQYYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVREDKKPEEATSSEQIADLYQAQ 776

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BLAST of FG534751 vs. SwissProt
Match: DNLI1_CAEEL (DNA ligase 1 OS=Caenorhabditis elegans GN=lig-1 PE=2 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 9.830e-38
Identity = 74/160 (46.25%), Postives = 109/160 (68.12%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            WLK+K+DYV+G+ DTLDLV +GA+ G G++ G Y  +L+ C+NP TEEY+SVC++ +GF+D    E  +I   DK + K P YYQ   T  PD  F P LV+E++ AD T+SP H AA GL    +GIS+RFPRF+    D+N ++ +++  ++EM+ +Q
Sbjct:  461 WLKMKKDYVDGVGDTLDLVVMGAYSGVGKRTGVYGGYLLGCYNPTTEEYESVCKIGTGFTDEDLAEQYKILQ-DKKIDKSPSYYQFDHTLKPDDTFSPYLVFEVKCADITISPRHKAASGLTDDGKGISLRFPRFLRIRDDKNSDDATSSEQVLEMYKNQ 619

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BLAST of FG534751 vs. SwissProt
Match: DNLI1_DICDI (DNA ligase 1 OS=Dictyostelium discoideum GN=lig1 PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 3.735e-37
Identity = 64/159 (40.25%), Postives = 107/159 (67.30%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPY-YQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            WLK+K+DY++G+ D+LDLVPIGAW+G G++ G Y  +L+AC++   EE+Q++C++ +GFSD              +   +  + +     PD+WF P  VWE+  AD ++SPVH AA G++ P++GI++RFPRFI    D++PE+ +++  +V+M+ +Q
Sbjct: 1018 WLKIKKDYMQGMTDSLDLVPIGAWYGKGKRTGTYGAYLLACYDENNEEFQTLCKIGTGFSDEQLTTFTSQLKPHLINQPRNQFRFSDSIKPDVWFSPSCVWEVLAADLSISPVHTAASGILDPNKGIALRFPRFIRIRPDKSPEDATSSDQVVDMYQNQ 1176

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BLAST of FG534751 vs. SwissProt
Match: DNLI_PICTO (DNA ligase OS=Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) GN=lig PE=3 SV=2)

HSP 1 Score: 152.14 bits (383), Expect = 2.421e-36
Identity = 68/163 (41.72%), Postives = 107/163 (65.64%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMD 497
            W+K KRDY   L+D+LDLV +GA+ G+GR+ G +   L+AC+N + + +++VC++ SGF+D    EM  +  GDK++ KKP    +   PD W  P LV EIRGA+ TVSPVH  A+ ++    G+++RFPR I    D+ PE+ +T  +I+EM+ +Q + ++
Sbjct:  422 WIKFKRDYQSELSDSLDLVVVGAFDGHGRRKGTFGALLLACYNSKDDTFETVCKLGSGFTDEMLSEMPRLL-GDKIVEKKPARVNSSMEPDHWIYPSLVLEIRGAEITVSPVHTCAMNIIEKGSGLALRFPRLIKPRDDKKPEDATTTNEIIEMYKAQKKVIE 583

[Close]

BLAST of FG534751 vs. SwissProt
Match: DNLI1_XENLA (DNA ligase 1 OS=Xenopus laevis GN=lig1 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 2.421e-36
Identity = 71/160 (44.38%), Postives = 107/160 (66.88%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQ--TGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            WLK+K+DY+EG+ DTLDLV IGA+ G G++ G Y  FL+A ++ E+EEYQ++C++ +GF+D   +E    F  D V+     YY+  +   PD WF P  VWE++ AD ++SPVH AA+GLV   +GIS+RFPRF+    D+ PEE + +  + +++  Q
Sbjct:  895 WLKLKKDYLEGVGDTLDLVVIGAYLGKGKRTGIYGGFLLASYDEESEEYQTICKIGTGFTDD-DLEKHYNFLKDHVIENPRSYYRWDSATEPDHWFDPVQVWEVKCADLSISPVHKAALGLVEDQKGISLRFPRFLRIRDDKKPEESTNSFQVADLYRKQ 1053

[Close]

BLAST of FG534751 vs. SwissProt
Match: DNLI1_SCHPO (DNA ligase 1 OS=Schizosaccharomyces pombe GN=cdc17 PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 3.162e-36
Identity = 73/176 (41.48%), Postives = 114/176 (64.77%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFS----DSFYIEMKEIFSGDKVLSKKPPYYQTGET----PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTEG 512
            WLKVK+DY+ G+ D+LDL+ IGA++G G++   Y  FL+ C++P+TE  QS+C++ +GFS    ++FY ++K+I     V+SKK  +Y   +     PD+WF P+ +WE+  AD ++SPV+ AAIG V   +GIS+RFPRFI    D++ E+ +T+  + E + SQ        EG
Sbjct:  591 WLKVKKDYLSGVGDSLDLIVIGAYYGKGKRTSVYGAFLLGCYDPDTETVQSICKLGTGFSEEHLETFYNQLKDI-----VISKKKDFYAHSDVPAHQPDVWFEPKYLWEVLAADLSLSPVYKAAIGYVQEDKGISLRFPRFIRIREDKSWEDATTSEQVSEFYRSQVAYSQKEKEG 761

[Close]

BLAST of FG534751 vs. SwissProt
Match: DNLI1_DROME (DNA ligase 1 OS=Drosophila melanogaster GN=CG5602 PE=1 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 3.162e-36
Identity = 73/160 (45.62%), Postives = 103/160 (64.38%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            WLK+K+DY+  + D+LDLV IG + G GR+ G Y  FL+AC++ E EEYQS+C++ +GF+D   ++    F G  V S    YY+   +  PD WF P  VWE++ AD ++SP+H AAIG+V   RGIS+RFPRFI    D+N E  + A  +  M+ SQ
Sbjct:  569 WLKLKKDYLSNVGDSLDLVVIGGYKGKGRRTGTYGGFLLACYDTENEEYQSICKIGTGFTDE-DLQTHSEFLGKHVTSAAKSYYRYDPSLEPDHWFEPVQVWEVKCADLSLSPIHRAAIGIVDGERGISLRFPRFIRIRDDKNSENATDANQVAHMYQSQ 727

[Close]

BLAST of FG534751 vs. SwissProt
Match: DNLI_THENV (DNA ligase OS=Thermoproteus neutrophilus (strain DSM 2338 / JCM 9278 / V24Sta) GN=lig PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.918e-33
Identity = 67/161 (41.61%), Postives = 109/161 (67.70%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSV-SDRNPEECSTAADIVEMFHSQTR 488
            W+K KRDY   + DT+DLV +GA++G G++AG Y  FL+A ++P+T+ + +VC+V SGF+D+   +M E+    K+   + P   +  TPD+WF PQ+V E+ GA+ T+SP+H   +G V P  G++IRFPRF     +D++PE+ +T A++VE++  Q +
Sbjct:  420 WIKYKRDYKSEMIDTVDLVVVGAFYGRGKRAGLYGAFLLAAYDPQTDMFYTVCKVGSGFTDADLKKMYEVLQPYKI-PHRHPRVVSKMTPDVWFTPQVVIEVIGAEITLSPLHTCCLGAVRPGVGLAIRFPRFTGRYRTDKSPEQATTPAEMVELYKRQKK 579

[Close]

BLAST of FG534751 vs. SwissProt
Match: DNLI_PYRAE (DNA ligase OS=Pyrobaculum aerophilum GN=lig PE=3 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 5.582e-33
Identity = 67/161 (41.61%), Postives = 106/161 (65.84%), Query Frame = 3

Query:    9 WLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSV-SDRNPEECSTAADIVEMFHSQTR 488
            W+K KRDY   + DT+DLV +GA+HG G++AG Y  FL+A ++P T+ + +VC+V SGF+D+   +M EI    K+  + P      E  D+WF PQ+V E+ GA+ T+SP+H   +G V P  G+++RFPRF     SD++PE+ +T A+++E++  Q +
Sbjct:  420 WIKYKRDYRSEMIDTVDLVVVGAFHGRGKRAGLYGAFLLAAYDPSTDMFYTVCKVGSGFTDADLKKMYEILQPFKIPHRHPRVVSKMEA-DVWFVPQVVIEVIGAEITLSPLHTCCLGAVRPGVGLAVRFPRFTGRYRSDKSPEQATTVAEMLELYKRQKK 579

[Close]

BLAST of FG534751 vs. TAIR peptide
Match: AT1G66730.1 (| Symbols: AtLIG6, LIG6 | DNA LIGASE 6 | chr1:24884991-24891823 FORWARD LENGTH=1396)

HSP 1 Score: 311.997 bits (798), Expect = 1.857e-85
Identity = 139/169 (82.25%), Postives = 158/169 (93.49%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQTRKMDVTTE 509
            D WLKVKRDYV+GL DTLDLVPIGAW+GNGRKAGWYSPFLMACFNPETEE+QSVCRVMSGFSD+FYIEMKE +S DK+L+KKPPYY+TGETPDMWF  ++VWEIRGADFTVSPVH A++GLVHPSRGIS+RFPRFI  V+DRNPEECSTA DI EMFH+QTRKM++T++
Sbjct: 1227 DSWLKVKRDYVDGLGDTLDLVPIGAWYGNGRKAGWYSPFLMACFNPETEEFQSVCRVMSGFSDAFYIEMKEFYSEDKILAKKPPYYRTGETPDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPRFISKVTDRNPEECSTATDIAEMFHAQTRKMNITSQ 1395

[Close]

BLAST of FG534751 vs. TAIR peptide
Match: AT1G08130.1 (| Symbols: ATLIG1, LIG1 | DNA ligase 1 | chr1:2542913-2547815 REVERSE LENGTH=790)

HSP 1 Score: 160.999 bits (406), Expect = 5.295e-40
Identity = 69/162 (42.59%), Postives = 112/162 (69.14%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSGDKVLSKKPPYYQTGET--PDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            + WLK+K+DY++ + D++DLVPI A+HG G++ G Y  FL+AC++ + EE+QS+C++ +GFSD+   E        +V++    YY+ G++  PD+WF P  VWE++ AD T+SPVH AA G+V P +GIS+RFPR +    D+ PEE +++  I +++ +Q
Sbjct:  616 NNWLKLKKDYMDSIGDSVDLVPIAAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLR-SQVIATPKQYYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVREDKKPEEATSSEQIADLYQAQ 776

[Close]

BLAST of FG534751 vs. TAIR peptide
Match: AT1G49250.1 (| Symbols: | ATP-dependent DNA ligase | chr1:18220533-18224256 FORWARD LENGTH=657)

HSP 1 Score: 157.918 bits (398), Expect = 4.483e-39
Identity = 70/164 (42.68%), Postives = 113/164 (68.90%), Query Frame = 3

Query:    3 DKWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACFNPETEEYQSVCRVMSGFSDSFYIEMKEIFSG--DKVLSKKPPYYQTGE--TPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHSQ 482
            + WLK+K+DY++ + D++DLVPI  +HG G++ G++  +L+AC++ + EE+QS+C++ + FSD   +E++++ S    KV++    YYQ      PD+W  P  VWE++ AD TVSPV+  AIG+V P +GIS+RFPR +    D+NPEE +T+  I EM+ +Q
Sbjct:  483 NNWLKLKKDYMDNIGDSVDLVPIATFHGRGKRTGFFGAYLLACYDVDKEEFQSICKIGTEFSD---VELQDLSSSLCSKVIATPKQYYQVDNDLNPDVWLEPTEVWEVKAADLTVSPVYREAIGIVDPDKGISLRFPRLVRIRKDKNPEEATTSDQIAEMYQAQ 643

[Close]

BLAST of FG534751 vs. TAIR peptide
Match: AT5G57160.1 (| Symbols: ATLIG4, LIG4 | DNA ligase IV | chr5:23155150-23161688 REVERSE LENGTH=1219)

HSP 1 Score: 55.8398 bits (133), Expect = 2.399e-8
Identity = 50/172 (29.07%), Postives = 78/172 (45.35%), Query Frame = 3

Query:    6 KWLKVKRDYVEGLNDTLDLVPIGAWHGNGRKAGWYSPFLMACF-----NPETEEYQSVCRVMSGFSD----SFYIEMKEIFSGDKVLSKKPP-YYQ----TGETPDMWFCPQLVWEIRGADFTVSPVHHAAIGLVHPSRGISIRFPRFICSVSDRNPEECSTAADIVEMFHS 479
            KW+K+K +Y+    D LD++ IG ++G+GR+ G  + FL+A       N     + S CRV +G SD    +   ++K  F  ++   K PP +YQ    + E PD+W        I      +  +       V P    S+RFPR      D+   EC      VE+ +S
Sbjct:  437 KWMKLKPEYIRAGAD-LDVLIIGGYYGSGRRGGEVAQFLVALADRAEANVYPRRFMSFCRVGTGLSDDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKERPDVWIDSPEKSIILSITSDIRTIRSEVF--VAP---YSLRFPRIDKVRYDKPWHECLDVQAFVELVNS 602

The following BLAST results are available for this feature:

BLAST of FG534751 vs. TrEMBL
BLAST of FG534751 vs. SwissProt
BLAST of FG534751 vs. TAIR peptide

BLAST of FG534751 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7KUD3_ARALY	1.090e-84	83.43	ATP dependent DNA ligase family protein OS=Arabido...	[more]
A5AGP3_VITVI	1.205e-83	85.03	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9H344_POPTR	4.578e-83	82.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TSX4_VITVI	5.979e-83	84.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9C9M5_ARATH	4.737e-80	74.73	DNA ligase OS=Arabidopsis thaliana GN=F4N21_14 PE=...	[more]
B9SPY8_RICCO	2.599e-78	80.72	DNA ligase OS=Ricinus communis GN=RCOM_0466830 PE=...	[more]
Q0JKB6_ORYSJ	6.202e-72	72.94	Os01g0685500 protein (Fragment) OS=Oryza sativa su...	[more]
B9EYN2_ORYSJ	4.465e-54	49.36	DNA ligase OS=Oryza sativa subsp. japonica GN=OsJ_...	[more]
B8A7M7_ORYSI	4.465e-54	49.36	DNA ligase OS=Oryza sativa subsp. indica GN=OsI_03...	[more]
C1E332_9CHLO	7.377e-49	56.55	DNA ligase OS=Micromonas sp. RCC299 GN=MICPUN_9721...	[more]

BLAST of FG534751 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DNLI3_SCHPO	7.770e-43	51.85	DNA ligase 3 OS=Schizosaccharomyces pombe GN=adl1 ...	[more]
DNLI1_ARATH	5.212e-39	42.59	DNA ligase 1 OS=Arabidopsis thaliana GN=At1g08130 ...	[more]
DNLI1_CAEEL	9.830e-38	46.25	DNA ligase 1 OS=Caenorhabditis elegans GN=lig-1 PE...	[more]
DNLI1_DICDI	3.735e-37	40.25	DNA ligase 1 OS=Dictyostelium discoideum GN=lig1 P...	[more]
DNLI_PICTO	2.421e-36	41.72	DNA ligase OS=Picrophilus torridus (strain ATCC 70...	[more]
DNLI1_XENLA	2.421e-36	44.38	DNA ligase 1 OS=Xenopus laevis GN=lig1 PE=2 SV=1	[more]
DNLI1_SCHPO	3.162e-36	41.48	DNA ligase 1 OS=Schizosaccharomyces pombe GN=cdc17...	[more]
DNLI1_DROME	3.162e-36	45.63	DNA ligase 1 OS=Drosophila melanogaster GN=CG5602 ...	[more]
DNLI_THENV	1.918e-33	41.61	DNA ligase OS=Thermoproteus neutrophilus (strain D...	[more]
DNLI_PYRAE	5.582e-33	41.61	DNA ligase OS=Pyrobaculum aerophilum GN=lig PE=3 S...	[more]

BLAST of FG534751 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G66730.1	1.857e-85	82.25	\| Symbols: AtLIG6, LIG6 \| DNA LIGASE 6 \| chr1:2488...	[more]
AT1G08130.1	5.295e-40	42.59	\| Symbols: ATLIG1, LIG1 \| DNA ligase 1 \| chr1:2542...	[more]
AT1G49250.1	4.483e-39	42.68	\| Symbols: \| ATP-dependent DNA ligase \| chr1:1822...	[more]
AT5G57160.1	2.399e-8	29.07	\| Symbols: ATLIG4, LIG4 \| DNA ligase IV \| chr5:231...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 62..77 score
None	No IPR available	PANTHER	PTHR10459	ATP-DEPENDENT DNA LIGASE FAMILY	coord: 1..169 score: 8.4
None	No IPR available	PANTHER	PTHR10459:SF10	DNA LIGASE I	coord: 1..169 score: 8.4
None	No IPR available	SEG	seg	seg	coord: 36..47 score
IPR012309	DNA ligase, ATP-dependent, C-terminal	PFAM	PF04679	DNA_ligase_A_C	coord: 33..137 score: 3.6
IPR012310	DNA ligase, ATP-dependent, central	PROFILE	PS50160	DNA_LIGASE_A3	coord: 1..45 score: 16
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 16..160 score: 4.8
IPR016027	Nucleic acid-binding, OB-fold-like	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 15..161 score: 6.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG534751 ID=FG534751; Name=FG534751; organism=Pisum sativum; type=EST; length=673bp
CAGACAAATGGTTAAAGGTCAAGCGAGATTATGTAGAAGGATTAAATGAT
ACTCTTGATTTAGTACCAATTGGTGCTTGGCATGGAAATGGAAGGAAAGC
TGGATGGTATAGTCCATTTCTCATGGCATGTTTCAATCCTGAAACTGAGG
AGTATCAAAGTGTTTGCCGAGTGATGTCTGGGTTCTCAGACTCTTTTTAC
ATAGAGATGAAAGAAATTTTTTCTGGGGACAAAGTCTTGTCAAAAAAGCC
ACCATATTATCAAACAGGAGAGACACCAGACATGTGGTTTTGTCCGCAAC
TTGTTTGGGAAATAAGAGGTGCAGACTTCACAGTATCACCAGTTCATCAT
GCTGCTATTGGTTTAGTTCATCCATCTCGAGGCATCTCAATCAGATTTCC
GAGATTTATTTGCAGCGTGTCAGACAGAAACCCAGAAGAATGTAGCACAG
CTGCCGATATAGTTGAAATGTTTCATTCGCAGACTCGGAAGATGGATGTG
ACAACTGAAGGTTGAACATTGGTTTGTTTCTTATGTATATACTATGTTCT
CATTGTAAAGTATTTCTAGGCAGCTTACTGAAGATGTTTTAATGTTTTGC
ATTTTTGTATACATTAGTGTATTATCTTTCTTGTCTTTTTTGTTCCCATG
TATATATGGCTTTGAAACTTGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003910	DNA ligase (ATP) activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006310	DNA recombination

Vocabulary: **INTERPRO**
Term	Definition
IPR012309	DNA_ligase_ATP-dep_C
IPR012310	DNA_ligase_ATP-dep_cent
IPR012340	NA-bd_OB-fold
IPR016027	Nucleic acid-binding, OB-fold-like

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