FG535172

EST Overview
NameFG535172
Unique NameFG535172
TypeEST
OrganismPisum sativum (pea)
Sequence length593
Libraries
Library NameType
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of FG535172 vs. TrEMBL
Match: C6TAU0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 1.753e-71
Identity = 142/203 (69.95%), Postives = 159/203 (78.33%), Query Frame = 1 
Query:    7 VETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPS-TQSNTKNLPQ-------KLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 591
            VET +L S+IADISIK+E                   +++  ++KE TVGSLSF+V STA+    +S TK +P        K P QKNS+KKPTVRAKVPFEKGYSQMDWLKLT+THPDLAGLKGQSN+RLISMDEVRKH+ EG+MWTVLKGRVYNISPYMKFHPGGVDMLMK VGKDCTSLFNKYHAWVNAEFLLEKC VGT
Sbjct:   18 VETNKLASDIADISIKEESSNASNTAQ----------DNALPNQKEATVGSLSFTVTSTASSRPNESTTKLVPTQANISSLKSPAQKNSVKKPTVRAKVPFEKGYSQMDWLKLTQTHPDLAGLKGQSNKRLISMDEVRKHQTEGQMWTVLKGRVYNISPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCFVGT 210
[Close]
BLAST of FG535172 vs. TrEMBL
Match: D7T352_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_64.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032154001 PE=4 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 1.641e-69
Identity = 135/202 (66.83%), Postives = 158/202 (78.22%), Query Frame = 1 
Query:   10 ETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATP------STQSNTKNLPQ--KLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 591
            E +ELV  I  I+I DE           GGFLWK  + ++++ KE TVGSLSF VI T++P      S Q+ TK+     K+P QK++ +KP  RAKVPFEKGYSQMDWLKLT+THPDLAGL GQSN+RLISM+EV++H++EG MWTVLKGRVYN+SPYMKFHPGGVDMLMK VGKDCTSLFNKYHAWVNAEFLLEKCLVGT
Sbjct:   22 EAQELVPGIGGITINDEFSSEVVGNGN-GGFLWKGKSPNNSTSKEATVGSLSFKVIDTSSPKKSNELSRQAATKDAGSSVKVP-QKSATRKPVARAKVPFEKGYSQMDWLKLTQTHPDLAGLNGQSNKRLISMNEVKQHRMEGSMWTVLKGRVYNLSPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 221
[Close]
BLAST of FG535172 vs. TrEMBL
Match: C6TJZ5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.656e-69
Identity = 138/203 (67.98%), Postives = 158/203 (77.83%), Query Frame = 1 
Query:    7 VETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPS-TQSNTKNLPQ-------KLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 591
            +ET +L S+IADISI++E                   + +  ++KE TVGSLSF+V STA+   ++S TK +P        K P QKNS+KKPTVRAKVPFEKGYSQMDWLKLT+THPDLAGLKGQS +RLISMDEVRKH+ EG+MWTVLKGRVYNISPYMKFHPGGV+MLMK VGKDCTSLFNKYHAWVNAEFLLEKC VGT
Sbjct:   18 LETNKLASDIADISIEEESSNATNTTQ----------DSALPNQKEATVGSLSFTVTSTASSRPSESTTKLVPAQANNSSLKSPAQKNSVKKPTVRAKVPFEKGYSQMDWLKLTQTHPDLAGLKGQSYKRLISMDEVRKHQTEGQMWTVLKGRVYNISPYMKFHPGGVNMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCFVGT 210
[Close]
BLAST of FG535172 vs. TrEMBL
Match: B9RVM7_RICCO (Flavohemoprotein B5/b5r, putative OS=Ricinus communis GN=RCOM_0965250 PE=3 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 1.878e-65
Identity = 132/203 (65.02%), Postives = 148/203 (72.91%), Query Frame = 1 
Query:    7 VETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTAT---PSTQSNTKNLPQ------KLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            +E   L S+I  +++KDE              LWKDG  +S + KE TVGSLSF+VI  A+   PS  S     P        L EQK S KKP  RAKVPFEKGYSQMDWLKLTRTHPDLAGL GQSN+R ISM+EV++H++EG MWTVLKGRVYN+SPYMKFHPGGVDMLMK VGKDCTSLFNKYHAWVN EFLLEKCLVG
Sbjct:   20 LEPNNLSSDIHAMTMKDECSNGISDSQS-SSILWKDGLPNSANSKEETVGSLSFNVIDAASFKRPSDLSGQVASPDTGTKVTSLKEQKASTKKPAPRAKVPFEKGYSQMDWLKLTRTHPDLAGLNGQSNKRRISMNEVKQHRIEGSMWTVLKGRVYNLSPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNEEFLLEKCLVG 221
[Close]
BLAST of FG535172 vs. TrEMBL
Match: B9GFC2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_641332 PE=3 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 2.711e-64
Identity = 128/205 (62.44%), Postives = 147/205 (71.71%), Query Frame = 1 
Query:    7 VETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDS-SNSKKEGTVGSLSFSVISTATPSTQS---------NTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 591
            +E + L S+I  I IKD            GG +W+D   S ++SK E TVGSLSF+VI  +     S         N     +   E+K S +KP  R+KVPFEKGYSQMDWLKLTRTHPDLAGLKGQSN+RLISMDEV+ H+ EG MWTVLKGRVYN+SPY+KFHPGG D+LMK VGKDCTSLFNKYHAWVNAEFLLEKCLVGT
Sbjct:   21 LEAENLASDIGGIIIKD-GFSNGTNSSQGGGVVWRDSLPSDADSKNERTVGSLSFNVIDASARGESSGVPRQVASGNAGTSAKNFDEKKVSARKPVARSKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLISMDEVKLHQTEGSMWTVLKGRVYNLSPYLKFHPGGADILMKAVGKDCTSLFNKYHAWVNAEFLLEKCLVGT 224
[Close]
BLAST of FG535172 vs. TrEMBL
Match: Q9LXD1_ARATH (At5g09680 OS=Arabidopsis thaliana GN=F17I14_130 PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 4.479e-59
Identity = 118/196 (60.20%), Postives = 137/196 (69.90%), Query Frame = 1 
Query:    1 PAVETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            P  E K+L S++  I++KD             G +WKD    S   KE T+GSLSF+V  +++   QSN  +   K P      +KP  R KVPFEKGYSQMDWLKLTRTHPDLAGLKG+SN+RLI MDEV+KH+    MWTVLKGRVYNISPYM FHPGGVDMLMK VG+D T LFNKYHAWVN + LLEKCLVG
Sbjct:   17 PDSEVKDLASDVGSITLKD-----GLDQQKSNGLIWKD---KSLPPKEETIGSLSFTVTDSSSSKKQSNESSETFKTPA-----RKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLEKCLVG 199
[Close]
BLAST of FG535172 vs. TrEMBL
Match: Q9FXX6_ARATH (Genomic DNA, chromosome 5, P1 clone: MTH16 OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 4.479e-59
Identity = 118/196 (60.20%), Postives = 137/196 (69.90%), Query Frame = 1 
Query:    1 PAVETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            P  E K+L S++  I++KD             G +WKD    S   KE T+GSLSF+V  +++   QSN  +   K P      +KP  R KVPFEKGYSQMDWLKLTRTHPDLAGLKG+SN+RLI MDEV+KH+    MWTVLKGRVYNISPYM FHPGGVDMLMK VG+D T LFNKYHAWVN + LLEKCLVG
Sbjct:   81 PDSEVKDLASDVGSITLKD-----GLDQQKSNGLIWKD---KSLPPKEETIGSLSFTVTDSSSSKKQSNESSETFKTPA-----RKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLEKCLVG 263
[Close]
BLAST of FG535172 vs. TrEMBL
Match: D7M265_ARALY (Cytochrome b5 domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487788 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 9.979e-59
Identity = 119/194 (61.34%), Postives = 137/194 (70.62%), Query Frame = 1 
Query:    7 VETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            +E K L S++  I++KD             G +WKD    S   KE TVGSLSF+VI +++   QSN  +   K P      +KP  R KVPFEKGYSQMDWLKLTRTHPDLAGLKG+SN+RLI MDEV+KH+    MWTVLKGRVYNISPYM FHPGGVDMLMK VG+D T LFNKYHAWVN + LLEKCLVG
Sbjct:   24 LEAKALASDVGSITLKDALDQQS------NGLIWKD---KSLPPKEETVGSLSFNVIDSSSLKKQSNESSETFKTPA-----RKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLIPMDEVKKHQSGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDMLLEKCLVG 203
[Close]
BLAST of FG535172 vs. TrEMBL
Match: D5ADU0_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.761e-55
Identity = 107/163 (65.64%), Postives = 122/163 (74.85%), Query Frame = 1 
Query:  112 DGNDSSNSKKEGTVGSLSFSVISTATPSTQSN----TKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            D +DS+  ++       +  +   A P  +S     T        +Q  S+ KP  RAKVPFEKGYSQMDWLKLT+THPDLAGLKG SNRRLISMDEV++HK EG++WTVLKGRVYNISPYMKFHPGG DMLMK  GKDCT+LFNKYHAWVNAEFLLEKCLVG
Sbjct:   34 DKSDSAEHREPKVSSDKNSKIFQVAPPKVKSTEMQETATAKGSTFKQPVSIAKPVRRAKVPFEKGYSQMDWLKLTQTHPDLAGLKGSSNRRLISMDEVKQHKSEGDIWTVLKGRVYNISPYMKFHPGGEDMLMKAAGKDCTALFNKYHAWVNAEFLLEKCLVG 196
[Close]
BLAST of FG535172 vs. TrEMBL
Match: Q84YL2_ORYSJ (Flavohemoprotein b5/b5R-like OS=Oryza sativa subsp. japonica GN=OSJNBa0086N05.132 PE=3 SV=2)

HSP 1 Score: 214.927 bits (546), Expect = 3.322e-54
Identity = 107/165 (64.85%), Postives = 124/165 (75.15%), Query Frame = 1 
Query:  112 DGN-DSSNSKKEGTVGSLSFSVISTATPSTQS-----NTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            +GN  ++NS K+ T  S+S S+   +   +       + +N    L  Q  S KKP  RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQ NRRLIS++EV++HK    +WTVLKGRVYNI+PYMKFHPGGVDMLMK  GKD T+LFNKYHAWVN EFLLEKCLVG
Sbjct:   48 EGNMQANNSIKDQTSNSISSSINRVSLEDSNGKESVLSRENTQSNLSSQPKSSKKPAARAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQLNRRLISLEEVKQHKTGDSIWTVLKGRVYNIAPYMKFHPGGVDMLMKAAGKDSTALFNKYHAWVNFEFLLEKCLVG 212
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_HUMAN (Cytochrome b5 reductase 4 OS=Homo sapiens GN=CYB5R4 PE=1 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.804e-26
Identity = 54/109 (49.54%), Postives = 78/109 (71.56%), Query Frame = 1 
Query:  268 RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISM--DEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            R+KVP ++G S MDW++LT++  DL GLKG    RLI +  +E++KH  + + W  ++G VYN+SPYM++HPGG D LM+  G D T LF++ H WVN E +L++CLVG
Sbjct:   23 RSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVG 127
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_BOVIN (Cytochrome b5 reductase 4 OS=Bos taurus GN=CYB5R4 PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.804e-26
Identity = 54/109 (49.54%), Postives = 78/109 (71.56%), Query Frame = 1 
Query:  268 RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISM--DEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            R+KVP ++G S MDW++LT++  DL GLKG    RLI +  +E++KH  + + W  ++G VYN+SPYM++HPGG D LM+  G D T LF++ H WVN E +L++CLVG
Sbjct:   23 RSKVPLKQGRSLMDWIRLTKSGKDLTGLKG----RLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTDLFDQVHRWVNYESMLKECLVG 127
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_MOUSE (Cytochrome b5 reductase 4 OS=Mus musculus GN=Cyb5r4 PE=2 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 8.887e-26
Identity = 52/107 (48.60%), Postives = 76/107 (71.03%), Query Frame = 1 
Query:  268 RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            R+KVP ++G S MDW++LT++  DL GLKG      ++ +E++KH  + + W  ++G VYN+SPYM++HPGG D LM+  G D T LFN+ H WVN E +L++CLVG
Sbjct:   23 RSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIE--VTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVG 127
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_RAT (Cytochrome b5 reductase 4 OS=Rattus norvegicus GN=Cyb5r4 PE=1 SV=2)

HSP 1 Score: 114.775 bits (286), Expect = 3.377e-25
Identity = 51/107 (47.66%), Postives = 74/107 (69.16%), Query Frame = 1 
Query:  268 RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            R+KVP ++G S MDW +LT++  D  GLKG      ++ +E++KH  + + W  ++G VYN+SPYM++HPGG D LM+  G D T LFN+ H WVN E +L++CLVG
Sbjct:   23 RSKVPLKQGRSLMDWFRLTKSGKDFTGLKGGLIE--VTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVG 127
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_XENTR (Cytochrome b5 reductase 4 OS=Xenopus tropicalis GN=cyb5r4 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 7.523e-25
Identity = 56/129 (43.41%), Postives = 85/129 (65.89%), Query Frame = 1 
Query:  214 NLP-QKLPEQKNSLKKPTV-RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISM--DEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            N+P Q  P   +  +   + R+KVP + G S MDW++LT++  DL GLKG    RLI +  +E+ +H  + + W  ++G VYNI+PYM++HPGG + LMK  G+D T LF++ H WVN E +L++CL+G
Sbjct:    3 NVPSQSFPAPSSQQRVAAIGRSKVPLKPGRSLMDWIRLTKSGKDLTGLKG----RLIDVTEEELAQHNKKEDCWICIRGMVYNITPYMEYHPGGEEELMKAAGRDGTDLFDQVHRWVNYESMLKECLIG 127
[Close]
BLAST of FG535172 vs. SwissProt
Match: NB5R4_DANRE (Cytochrome b5 reductase 4 OS=Danio rerio GN=cyb5r4 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.369e-24
Identity = 52/109 (47.71%), Postives = 75/109 (68.81%), Query Frame = 1 
Query:  268 RAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISM--DEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            R KV  + G+S +DW++LT++  DL GL+G    RLI +  DE++KH  + + WT ++G VYN+S YM FHPGG + LM+  G D T LF++ H WVN E +L++CLVG
Sbjct:   25 RNKVVLKPGHSLLDWIRLTKSGQDLTGLRG----RLIEVTEDELKKHNTKKDCWTCIRGMVYNLSAYMDFHPGGEEELMRAAGIDSTDLFDEVHRWVNYESMLKECLVG 129
[Close]
BLAST of FG535172 vs. SwissProt
Match: YDAA_SCHPO (Uncharacterized protein C1F12.10c OS=Schizosaccharomyces pombe GN=SPAC1F12.10c PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.421e-17
Identity = 42/118 (35.59%), Postives = 71/118 (60.17%), Query Frame = 1 
Query:  241 KNSLKKPT--VRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            ++S K+P    R K     G+SQ++W KL  +  +L+G++       ++ +E+ KHK + + W  ++G+VYN+S Y+ +HP G   ++   G+D T +F K+HAWVN E LL+   VG
Sbjct:   30 EHSEKQPRRDFRVKKYVAPGFSQLNWSKLVASGQNLSGVEKPIP---VTKEELAKHKTKEDCWIAIRGKVYNVSAYLPYHPAGQKRILDYAGRDATVIFMKFHAWVNEEALLKTSFVG 144
[Close]
BLAST of FG535172 vs. SwissProt
Match: YMW3_YEAST (Uncharacterized heme-binding protein YMR073C OS=Saccharomyces cerevisiae GN=YMR073C PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 9.848e-17
Identity = 64/200 (32.00%), Postives = 92/200 (46.00%), Query Frame = 1 
Query:  112 DGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDL----------------------------AGLKGQSNRRLISMDE-----------VRKH-KLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMK-TVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            DG +   S  +  V   +   +S A P+  S     P  +P  K+S +KP VR K+  + G+S +DW  LT    +                             + LK Q   + I + +           V+KH K E E+W V+ G+VY+IS Y+KFHPGG D+L+K     D  + FNKYH WVN E LL+ C +G
Sbjct:    4 DGMNRDVSNSKPNVRFAAPQRLSVAHPAISS-----PLHMPMSKSS-RKPLVRTKIRLDPGHSALDWHSLTSNPANYYTKFVSLQLIQDLLDDPVFQKDNFKFSPSQLKNQLLVQKIPLYKIMPPLRINRKIVKKHCKGEDELWCVINGKVYDISSYLKFHPGGTDILIKHRNSDDLITYFNKYHQWVNYEKLLQVCFIG 197
[Close]
BLAST of FG535172 vs. SwissProt
Match: YJ83_SCHPO (Uncharacterized heme-binding protein C330.03c OS=Schizosaccharomyces pombe GN=SPCC330.03c PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.803e-14
Identity = 41/130 (31.54%), Postives = 73/130 (56.15%), Query Frame = 1 
Query:  199 QSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            ++N+K+      E+K   KK   + K+P   G++Q DW  L  +  +L+G++   +   ++ +E+ KH    + W  ++G+VYN++ Y+ +HP G   ++K  G D T  + K+H WVN E LL+   VG
Sbjct:   20 EANSKDTKGLKEERKR--KKRKNKYKIP--PGHTQQDWDALVASGKNLSGVESPIS---VTAEELAKHCSPDDCWMAIRGKVYNVTAYLPYHPVGPKKILKHSGVDATKPYLKHHDWVNEEELLKTSFVG 142
[Close]
BLAST of FG535172 vs. SwissProt
Match: FAD5B_DICDI (Delta(5) fatty acid desaturase B OS=Dictyostelium discoideum GN=fadB PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.775e-11
Identity = 27/69 (39.13%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  370 RLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEK 576
            +L + DEV KH  + ++W ++ G+VYNI+ ++  HPGG D+L+ + G+D T+LF  YH   +  + L K
Sbjct:   13 KLYTWDEVSKHNQKNDLWIIVDGKVYNITKWVPLHPGGEDILLLSAGRDATNLFESYHPMTDKHYSLIK 81
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT5G09680.2 (| Symbols: RLF | reduced lateral root formation | chr5:2999362-3000185 REVERSE LENGTH=211)

HSP 1 Score: 231.106 bits (588), Expect = 3.395e-61
Identity = 118/196 (60.20%), Postives = 137/196 (69.90%), Query Frame = 1 
Query:    1 PAVETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            P  E K+L S++  I++KD             G +WKD    S   KE T+GSLSF+V  +++   QSN  +   K P      +KP  R KVPFEKGYSQMDWLKLTRTHPDLAGLKG+SN+RLI MDEV+KH+    MWTVLKGRVYNISPYM FHPGGVDMLMK VG+D T LFNKYHAWVN + LLEKCLVG
Sbjct:   17 PDSEVKDLASDVGSITLKD-----GLDQQKSNGLIWKD---KSLPPKEETIGSLSFTVTDSSSSKKQSNESSETFKTPA-----RKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLEKCLVG 199
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT5G09680.1 (| Symbols: RLF | reduced lateral root formation | chr5:2999362-3000185 REVERSE LENGTH=211)

HSP 1 Score: 231.106 bits (588), Expect = 3.395e-61
Identity = 118/196 (60.20%), Postives = 137/196 (69.90%), Query Frame = 1 
Query:    1 PAVETKELVSNIADISIKDEXXXXXXXXXXXGGFLWKDGNDSSNSKKEGTVGSLSFSVISTATPSTQSNTKNLPQKLPEQKNSLKKPTVRAKVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            P  E K+L S++  I++KD             G +WKD    S   KE T+GSLSF+V  +++   QSN  +   K P      +KP  R KVPFEKGYSQMDWLKLTRTHPDLAGLKG+SN+RLI MDEV+KH+    MWTVLKGRVYNISPYM FHPGGVDMLMK VG+D T LFNKYHAWVN + LLEKCLVG
Sbjct:   17 PDSEVKDLASDVGSITLKD-----GLDQQKSNGLIWKD---KSLPPKEETIGSLSFTVTDSSSSKKQSNESSETFKTPA-----RKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKGESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLEKCLVG 199
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT5G53560.1 (| Symbols: ATB5-A, B5 #2, ATCB5-E, CB5-E | cytochrome B5 isoform E | chr5:21759628-21760353 FORWARD LENGTH=134)

HSP 1 Score: 63.929 bits (154), Expect = 7.177e-11
Identity = 26/76 (34.21%), Postives = 48/76 (63.16%), Query Frame = 1 
Query:  361 SNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            S+R+++S +EV KH    + W ++ G+VY+++P+M  HPGG ++L+ + GKD T+ F        A  +++K  +G
Sbjct:    3 SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDKYFIG 78
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT3G61580.1 (| Symbols: | Fatty acid/sphingolipid desaturase | chr3:22786253-22787602 FORWARD LENGTH=449)

HSP 1 Score: 62.3882 bits (150), Expect = 2.088e-10
Identity = 24/73 (32.88%), Postives = 48/73 (65.75%), Query Frame = 1 
Query:  358 QSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYH---AWVNAEFL 567
            ++ ++ I+ ++++KH   G++W  ++G+VYN+S ++K HPGG  +++  VG+D T  F  +H   AW + + L
Sbjct:    4 ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIAFHPGTAWHHLDHL 76
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT1G26340.1 (| Symbols: B5 #6, ATCB5-A, CB5-A | cytochrome B5 isoform A | chr1:9113992-9114755 FORWARD LENGTH=135)

HSP 1 Score: 62.3882 bits (150), Expect = 2.088e-10
Identity = 29/73 (39.73%), Postives = 42/73 (57.53%), Query Frame = 1 
Query:  370 RLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            +L SM+E   H  + + W V+ G+VY++S YM  HPGG D+L+   GKD T  F       +A  L+EK  +G
Sbjct:    6 KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDARELMEKYFIG 78
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT2G46210.1 (| Symbols: | Fatty acid/sphingolipid desaturase | chr2:18977542-18978891 FORWARD LENGTH=449)

HSP 1 Score: 61.2326 bits (147), Expect = 4.652e-10
Identity = 25/73 (34.25%), Postives = 46/73 (63.01%), Query Frame = 1 
Query:  358 QSNRRLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYH---AWVNAEFL 567
            Q+ +R ++ ++++KH   G++W  ++G+VY++S ++K HPGG   ++   G+D T  F  YH   AW + E L
Sbjct:    4 QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIAYHPGTAWHHLEKL 76
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT2G46650.1 (| Symbols: B5 #1, ATCB5-C, CB5-C | cytochrome B5 isoform C | chr2:19151807-19152394 FORWARD LENGTH=132)

HSP 1 Score: 58.5362 bits (140), Expect = 3.016e-9
Identity = 27/72 (37.50%), Postives = 45/72 (62.50%), Query Frame = 1 
Query:  373 LISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            LIS  +V KHK + + W ++ G+VY+IS +M  HPGG ++L+   GKD +  F   +   +A+ L++K  +G
Sbjct:    4 LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKKYCIG 75
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT1G77760.1 (| Symbols: NIA1, GNR1, NR1 | nitrate reductase 1 | chr1:29236005-29239367 REVERSE LENGTH=917)

HSP 1 Score: 57.7658 bits (138), Expect = 5.144e-9
Identity = 26/73 (35.62%), Postives = 41/73 (56.16%), Query Frame = 1 
Query:  370 RLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            ++ S+ EVRKH      W ++ G +Y+ + ++K HPGG D ++   G DCT  F   H+   A+ LLE   +G
Sbjct:  546 KMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIHS-DKAKKLLEDYRIG 617
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT2G32720.1 (| Symbols: B5 #4, ATCB5-B, CB5-B | cytochrome B5 isoform B | chr2:13877013-13878447 REVERSE LENGTH=134)

HSP 1 Score: 57.3806 bits (137), Expect = 6.718e-9
Identity = 24/73 (32.88%), Postives = 44/73 (60.27%), Query Frame = 1 
Query:  370 RLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            ++ ++ EV +H    + W V+ G+VYN++ +++ HPGG D+L+ + GKD T  F       +A  ++E+  VG
Sbjct:    6 KIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVG 78
[Close]
BLAST of FG535172 vs. TAIR peptide
Match: AT1G37130.1 (| Symbols: NIA2, B29, NIA2-1, CHL3, NR, NR2, ATNR2 | nitrate reductase 2 | chr1:14158617-14161652 FORWARD LENGTH=917)

HSP 1 Score: 53.9138 bits (128), Expect = 7.428e-8
Identity = 24/73 (32.88%), Postives = 40/73 (54.79%), Query Frame = 1 
Query:  370 RLISMDEVRKHKLEGEMWTVLKGRVYNISPYMKFHPGGVDMLMKTVGKDCTSLFNKYHAWVNAEFLLEKCLVG 588
            ++ SM EV+KH      W ++ G +Y+ + ++  HPGG D ++   G DCT  F   H+   A+ +LE   +G
Sbjct:  543 KMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAIHS-DKAKKMLEDYRIG 614
The following BLAST results are available for this feature:
BLAST of FG535172 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6TAU0_SOYBN1.753e-7169.95Putative uncharacterized protein OS=Glycine max PE... [more]
D7T352_VITVI1.641e-6966.83Whole genome shotgun sequence of line PN40024, sca... [more]
C6TJZ5_SOYBN3.656e-6967.98Putative uncharacterized protein OS=Glycine max PE... [more]
B9RVM7_RICCO1.878e-6565.02Flavohemoprotein B5/b5r, putative OS=Ricinus commu... [more]
B9GFC2_POPTR2.711e-6462.44Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9LXD1_ARATH4.479e-5960.20At5g09680 OS=Arabidopsis thaliana GN=F17I14_130 PE... [more]
Q9FXX6_ARATH4.479e-5960.20Genomic DNA, chromosome 5, P1 clone: MTH16 OS=Arab... [more]
D7M265_ARALY9.979e-5961.34Cytochrome b5 domain-containing protein OS=Arabido... [more]
D5ADU0_PICSI1.761e-5565.64Putative uncharacterized protein OS=Picea sitchens... [more]
Q84YL2_ORYSJ3.322e-5464.85Flavohemoprotein b5/b5R-like OS=Oryza sativa subsp... [more]
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BLAST of FG535172 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
NB5R4_HUMAN6.804e-2649.54Cytochrome b5 reductase 4 OS=Homo sapiens GN=CYB5R... [more]
NB5R4_BOVIN6.804e-2649.54Cytochrome b5 reductase 4 OS=Bos taurus GN=CYB5R4 ... [more]
NB5R4_MOUSE8.887e-2648.60Cytochrome b5 reductase 4 OS=Mus musculus GN=Cyb5r... [more]
NB5R4_RAT3.377e-2547.66Cytochrome b5 reductase 4 OS=Rattus norvegicus GN=... [more]
NB5R4_XENTR7.523e-2543.41Cytochrome b5 reductase 4 OS=Xenopus tropicalis GN... [more]
NB5R4_DANRE6.369e-2447.71Cytochrome b5 reductase 4 OS=Danio rerio GN=cyb5r4... [more]
YDAA_SCHPO4.421e-1735.59Uncharacterized protein C1F12.10c OS=Schizosacchar... [more]
YMW3_YEAST9.848e-1732.00Uncharacterized heme-binding protein YMR073C OS=Sa... [more]
YJ83_SCHPO7.803e-1431.54Uncharacterized heme-binding protein C330.03c OS=S... [more]
FAD5B_DICDI2.775e-1139.13Delta(5) fatty acid desaturase B OS=Dictyostelium ... [more]
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BLAST of FG535172 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT5G09680.23.395e-6160.20| Symbols: RLF | reduced lateral root formation | ... [more]
AT5G09680.13.395e-6160.20| Symbols: RLF | reduced lateral root formation | ... [more]
AT5G53560.17.177e-1134.21| Symbols: ATB5-A, B5 #2, ATCB5-E, CB5-E | cytochr... [more]
AT3G61580.12.088e-1032.88| Symbols: | Fatty acid/sphingolipid desaturase |... [more]
AT1G26340.12.088e-1039.73| Symbols: B5 #6, ATCB5-A, CB5-A | cytochrome B5 i... [more]
AT2G46210.14.652e-1034.25| Symbols: | Fatty acid/sphingolipid desaturase |... [more]
AT2G46650.13.016e-937.50| Symbols: B5 #1, ATCB5-C, CB5-C | cytochrome B5 i... [more]
AT1G77760.15.144e-935.62| Symbols: NIA1, GNR1, NR1 | nitrate reductase 1 |... [more]
AT2G32720.16.718e-932.88| Symbols: B5 #4, ATCB5-B, CB5-B | cytochrome B5 i... [more]
AT1G37130.17.428e-832.88| Symbols: NIA2, B29, NIA2-1, CHL3, NR, NR2, ATNR2... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5PRINTSPR00363CYTOCHROMEB5coord: 148..158
score: 3.1e-05coord: 158..172
score: 3.1e-05coord: 186..197
score: 3.1
IPR001199Cytochrome b5GENE3D3.10.120.10no descriptioncoord: 122..197
score: 5.1
IPR001199Cytochrome b5PFAMPF00173Cyt-b5coord: 126..197
score: 7.5
IPR001199Cytochrome b5PROFILEPS50255CYTOCHROME_B5_2coord: 123..197
score: 20
IPR001199Cytochrome b5SUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 122..197
score: 4.5
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 154..161
score
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 126..196
score: 1.2
NoneNo IPR availablePANTHERPTHR19370:SF1FLAVOHEMOPROTEIN B5/B5Rcoord: 126..196
score: 1.2
NoneNo IPR availableSEGsegsegcoord: 21..31
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG535172 ID=FG535172; Name=FG535172; organism=Pisum sativum; type=EST; length=593bp
CCCGCAGTGGAAACTAAGGAGCTTGTTTCAAATATTGCTGATATTTCCAT
AAAGGACGAATCATCAAATGCAAGTAGTAGTAATCACAACAATGGTGGTT
TCTTATGGAAGGATGGTAATGATTCTTCCAACTCTAAAAAGGAGGGAACA
GTTGGTTCTTTGTCTTTCAGTGTAATCAGCACGGCAACTCCTTCAACTCA
AAGTAATACTAAGAACTTGCCTCAGAAGTTGCCAGAACAAAAGAATTCTC
TTAAAAAGCCAACAGTTCGAGCCAAAGTTCCTTTTGAGAAGGGTTATAGT
CAAATGGACTGGCTCAAGCTTACTCGAACTCATCCTGACCTTGCAGGTCT
AAAAGGACAGTCAAACAGGAGACTTATATCTATGGATGAAGTAAGAAAAC
ATAAATTAGAAGGCGAAATGTGGACTGTATTAAAAGGCCGTGTTTACAAT
ATATCACCATATATGAAGTTTCACCCTGGAGGTGTTGATATGTTGATGAA
AACTGTGGGAAAAGATTGCACATCTCTATTCAATAAATACCATGCTTGGG
TTAACGCCGAATTCTTACTGGAGAAATGCCTTGTAGGTACTTT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
IPR018506Cyt_B5_heme-BS